Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,651,665 G→T 100% G210G (GGC→GGA)  hscA ← DnaK‑like molecular chaperone specific for IscU

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,651,6650GT99.5% 574.9 / ‑4.2 182G210G (GGC→GGA) hscADnaK‑like molecular chaperone specific for IscU
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (119/62);  total (120/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 182 total aligned reads displayed.

TACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCGCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTGACGCTGGGC  >  CP009273/2651520‑2651808
                                                                                                                                                 |                                                                                                                                               
tACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTcc                                                                                                                                               >  1:2140052/1‑148 (MQ=255)
tACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTcc                                                                                                                                               >  2:1338155/1‑148 (MQ=255)
tACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTcc                                                                                                                                               <  2:2538854/148‑1 (MQ=255)
tACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTcc                                                                                                                                               >  1:26166/1‑148 (MQ=255)
       aGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAgg                                                                                                                                        <  1:1827736/148‑1 (MQ=255)
        ggAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGAGGGTCGAAATCAGCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGt                                                                                                                                       <  1:3117775/148‑1 (MQ=255)
        ggAAGGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGt                                                                                                                                       <  1:2311752/148‑1 (MQ=255)
         gAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTc                                                                                                                                      >  1:1049235/1‑148 (MQ=255)
         gAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTc                                                                                                                                      >  2:2289911/1‑148 (MQ=255)
         gAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTc                                                                                                                                      >  2:2684482/1‑148 (MQ=255)
                   cTGCTCGCGATAGTAAGCCGCCAGCAGATGATCGAAATCAGCGCGGCGTAGCGCGGAATAACCGCCGTGTGCCTGAAATGAAAACACGCCGCGACTTAAGCGCATAATGTAAATTTCAAACTTCCCTCCACCGTGGTCATAAACGGCg                                                                                                                            <  2:2750185/148‑1 (MQ=255)
                     gCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGAt                                                                                                                          >  2:875671/1‑148 (MQ=255)
                       tCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCa                                                                                                                        >  1:2741629/1‑148 (MQ=255)
                        cgcgAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCAc                                                                                                                       >  2:2424870/1‑148 (MQ=255)
                            aaTGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCt                                                                                                                   >  2:2731193/1‑148 (MQ=255)
                            aaTGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCt                                                                                                                   >  1:382298/1‑148 (MQ=255)
                            aaTGTAATCCGCCAGCAGATGGTCGAAATCAGCGCCGCCGAGCGCGGAATCACCGCGGGTTGCCAGCACTACAACCCCGCCGCGAATTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCt                                                                                                                   <  1:3122399/148‑1 (MQ=255)
                              tGTAATCCGCCATCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATAAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTc                                                                                                                 >  1:3071534/1‑148 (MQ=255)
                              tGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTc                                                                                                                 >  1:618979/1‑148 (MQ=255)
                              tGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTc                                                                                                                 >  1:549788/1‑148 (MQ=255)
                               gTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTcc                                                                                                                >  1:1186106/1‑148 (MQ=255)
                                tAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCt                                                                                                               >  1:1819793/1‑148 (MQ=255)
                                tAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCt                                                                                                               >  2:2502729/1‑148 (MQ=255)
                                   tCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGAc                                                                                                            >  2:914490/1‑148 (MQ=255)
                                   tCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGAc                                                                                                            >  1:2574595/1‑148 (MQ=255)
                                        cagcagATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGaa                                                                                                       <  1:212433/148‑1 (MQ=255)
                                          gcagATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATc                                                                                                     >  2:1965119/1‑148 (MQ=255)
                                           cagATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATcc                                                                                                    >  1:1280886/1‑148 (MQ=255)
                                           cagATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATcc                                                                                                    <  1:2863473/148‑1 (MQ=255)
                                                  tCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGt                                                                                             <  2:764108/148‑1 (MQ=255)
                                                   ctaaaTCATCGCCGCCGAGCGCGTAATCACCGCCGGTTGCCAGCACTACAAACACGCCGCGACTTAAGCGCAGATTGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTa                                                                                            <  1:2535330/146‑1 (MQ=255)
                                                     aaaTCAGCGCCGCCGAGCGCGGAAACACCGCGGGTTGCCACCAATACAAACACCCCGCGACTTAAGCGAAGAATGTAAATAACAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAgg                                                                                          <  1:2249174/148‑1 (MQ=255)
                                                      aaTCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGc                                                                                         >  1:737836/1‑148 (MQ=255)
                                                       atcatcGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCg                                                                                        >  1:1600775/1‑148 (MQ=255)
                                                        tcatcGCCGCCGAGCGCGGAATCACCGGCGGTTGCCAGAACTACAAACCCGCCTCTACTTAAGGGCAGAATGGAAATTTCAAACGTCCCTGCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCATCCCGTAGGCGa                                                                                       <  2:2835091/148‑1 (MQ=255)
                                                        tcatcGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGaa                                                                                                       >  2:2450125/1‑132 (MQ=255)
                                                        tcatcGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGa                                                                                       >  1:1532259/1‑148 (MQ=255)
                                                         catcGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGAt                                                                                      >  1:3181825/1‑148 (MQ=255)
                                                         catcGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGAt                                                                                      >  1:3180911/1‑148 (MQ=255)
                                                         catcGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGAt                                                                                      <  1:1837698/148‑1 (MQ=255)
                                                         catcGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGAt                                                                                      >  2:176448/1‑148 (MQ=255)
                                                         cagcgccgcCGAGCGCGGAACCCCCGCCGGTTGCCAGCACTTAAAACACGCCGCGACTAAAGCGCAGAATGGAAATAACAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGAt                                                                                      <  1:2775467/145‑1 (MQ=255)
                                                          atcGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATa                                                                                     >  2:2391694/1‑148 (MQ=255)
                                                           tcGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAg                                                                                    >  1:1784483/1‑148 (MQ=255)
                                                                    aGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGcgg                                                                           >  1:2317995/1‑148 (MQ=255)
                                                                         ggAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGcggtcggt                                                                      >  1:1846736/1‑148 (MQ=255)
                                                                           aaTCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTc                                                                    >  1:777413/1‑148 (MQ=255)
                                                                           aaTCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTc                                                                    >  2:1256783/1‑148 (MQ=255)
                                                                             tCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGt                                                                  <  2:1454549/148‑1 (MQ=255)
                                                                             tCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGt                                                                  >  1:375998/1‑148 (MQ=255)
                                                                             tCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGt                                                                  >  1:2041380/1‑148 (MQ=255)
                                                                             tCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGt                                                                  >  1:2814999/1‑148 (MQ=255)
                                                                                ccgccgGTTGCCAGCACTACAAACCCGCCGCGACTTAAGCGCAGAATGTAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTtaa                                                               <  1:1715577/148‑1 (MQ=255)
                                                                                 cgcggtttGCCAGAAATACAAACCCGCCGCTATTTAAGCGCATAATGTAAATATCACACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTtaag                                                              <  1:2777188/142‑1 (MQ=255)
                                                                                 cgccgGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGGtaag                                                              >  2:3191352/1‑148 (MQ=255)
                                                                                  gccgGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTtaagt                                                             <  1:2382307/148‑1 (MQ=255)
                                                                                   ccgGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTtaagta                                                            <  1:2673919/148‑1 (MQ=255)
                                                                                    cgGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTtaagtaa                                                           <  2:1049235/148‑1 (MQ=255)
                                                                                        tGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAgcgc                                                       >  1:3254254/1‑148 (MQ=255)
                                                                                        tGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAgcgc                                                       >  1:3254155/1‑148 (MQ=255)
                                                                                        tGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAgcgc                                                       >  1:3254851/1‑148 (MQ=255)
                                                                                        tGCCACCACTTCAACCACGCCGCGACTTACGCGCATAATGCAAATATCAAACGTCCCTCCACCGAGGTCATACACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAgcgc                                                       <  1:34764/148‑1 (MQ=255)
                                                                                          ccAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAg                                                     <  2:2314470/148‑1 (MQ=255)
                                                                                           cAGAACTTCAAACACGCCGCTACTTAAGCGCAGAATGGAATTATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAgg                                                    <  1:1635048/148‑1 (MQ=255)
                                                                                             gCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGAc                                                  >  2:313390/1‑148 (MQ=255)
                                                                                             gCACTACAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGAc                                                  >  2:618599/1‑148 (MQ=255)
                                                                                              cACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGTTATCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACg                                                 <  2:2040727/148‑1 (MQ=255)
                                                                                              cACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACg                                                 >  2:414828/1‑148 (MQ=255)
                                                                                                  tCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGaa                                             <  2:611512/148‑1 (MQ=255)
                                                                                                     aaCACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGc                                          >  1:1190670/1‑148 (MQ=255)
                                                                                                      acacGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGcc                                         >  1:332084/1‑148 (MQ=255)
                                                                                                       cacGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGccc                                        >  1:2543979/1‑148 (MQ=255)
                                                                                                                 cTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGAcgcg                              >  2:1003819/1‑148 (MQ=255)
                                                                                                                  ttAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACgcgc                             <  2:1326574/148‑1 (MQ=255)
                                                                                                                  ttAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACgcgc                             >  1:507567/1‑148 (MQ=255)
                                                                                                                  ttAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACAGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACgcgc                             >  2:1156478/1‑148 (MQ=255)
                                                                                                                     aGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGcgccgc                          >  1:917768/1‑148 (MQ=255)
                                                                                                                       cgcAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGt                        >  1:2978666/1‑148 (MQ=255)
                                                                                                                       cgcAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGt                        >  2:3002093/1‑148 (MQ=255)
                                                                                                                       cgcAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGt                        >  2:2724729/1‑148 (MQ=255)
                                                                                                                        gcAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTc                       <  2:217331/148‑1 (MQ=255)
                                                                                                                         cAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCt                      >  2:1464384/1‑148 (MQ=255)
                                                                                                                         cAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCt                      <  2:2814999/148‑1 (MQ=255)
                                                                                                                           gAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCttt                    >  1:1302281/1‑148 (MQ=255)
                                                                                                                           gAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCttt                    >  1:1301997/1‑148 (MQ=255)
                                                                                                                           gAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCttt                    >  1:2683176/1‑148 (MQ=255)
                                                                                                                             aTGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTgg                  >  1:2449345/1‑148 (MQ=255)
                                                                                                                             aTGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTGTgg                  >  1:3065337/1‑148 (MQ=255)
                                                                                                                              tGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGt                 >  2:2957802/1‑148 (MQ=255)
                                                                                                                                 aaaTATCACCCGTCCCTCCACCGAGGTCAGACCCGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGcc              <  2:377667/148‑1 (MQ=255)
                                                                                                                                 aaaTATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGcc              <  2:1784483/148‑1 (MQ=255)
                                                                                                                                  aaTATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGccc             >  1:108244/1‑148 (MQ=255)
                                                                                                                                   atatCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCt            >  2:2240311/1‑148 (MQ=255)
                                                                                                                                   atatCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCt            >  2:2238733/1‑148 (MQ=255)
                                                                                                                                   atatCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCt            >  1:2597895/1‑148 (MQ=255)
                                                                                                                                   atatCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCt            >  2:783045/1‑148 (MQ=255)
                                                                                                                                    tatCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTg           <  2:1800872/148‑1 (MQ=255)
                                                                                                                                     atCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTGa          <  2:3269492/148‑1 (MQ=255)
                                                                                                                                           cGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTGACGCTgg    >  1:28956/1‑148 (MQ=255)
                                                                                                                                             tCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTGACGCTGGGc  >  2:1388703/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
TACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCGCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTGACGCTGGGC  >  CP009273/2651520‑2651808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCTACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCGCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTGACGCTGGGCATCGTC  >  CP009273/2651518‑2651814
                                                                                                                                                   |                                                                                                                                                     
GCTACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAGATATCAAACGTCCCTCC                                                                                                                                                     >  SRR19973070.2154082/1‑150 (MQ=60)
GCTACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCC                                                                                                                                                     >  SRR19973070.2945407/1‑150 (MQ=60)
GCTACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCC                                                                                                                                                     >  SRR19973070.26522/1‑150 (MQ=60)
GCTACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCC                                                                                                                                                     >  SRR19973070.2154321/1‑150 (MQ=60)
         AGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTC                                                                                                                                            >  SRR19973070.1056620/1‑150 (MQ=60)
         AGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTC                                                                                                                                            <  SRR19973070.1840303/150‑1 (MQ=60)
          GGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCA                                                                                                                                           <  SRR19973070.3138656/150‑1 (MQ=60)
          GGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGAGGGTCGAAATCAGCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCA                                                                                                                                           <  SRR19973070.3137893/150‑1 (MQ=60)
          GGAAGGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCA                                                                                                                                           <  SRR19973070.2327120/150‑1 (MQ=60)
            AATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATA                                                                                                                                         >  SRR19973070.380433/1‑150 (MQ=60)
            AATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATA                                                                                                                                         >  SRR19973070.2556388/1‑150 (MQ=60)
            AATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCGCCACCGAGGTCATA                                                                                                                                         >  SRR19973070.2689123/1‑150 (MQ=60)
                CCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACG                                                                                                                                     >  SRR19973070.534485/1‑150 (MQ=60)
                 CCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCTAAATCATCGCCGCCGAGCGCGTAATAACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGG                                                                                                                                    <  SRR19973070.3187013/150‑1 (MQ=60)
                       GCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCA                                                                                                                              >  SRR19973070.2759264/1‑150 (MQ=60)
                       GCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCA                                                                                                                              >  SRR19973070.2759273/1‑150 (MQ=60)
                         TCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGTAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCATAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACG                                                                                                                            <  SRR19973070.2696150/150‑1 (MQ=60)
                           GCTAATGTAATCCGCCAGCAGTTGGTCGAAATCAGCGCCGCCGAGCGCGTAATCACCGCCGGTTGCCAGCACTTCAAACCCGCCGCGACTTAAGCGCATAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCC                                                                                                                          <  SRR19973070.2866952/150‑1 (MQ=60)
                            CGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCT                                                                                                                         >  SRR19973070.385105/1‑150 (MQ=60)
                            CGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCAGAAACGGCGATCACGCCT                                                                                                                         >  SRR19973070.1724969/1‑150 (MQ=60)
                              AATGTAATCCGCCATCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATAAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTC                                                                                                                       >  SRR19973070.3091244/1‑150 (MQ=60)
                              AATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTC                                                                                                                       >  SRR19973070.3091251/1‑150 (MQ=60)
                              AATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTC                                                                                                                       >  SRR19973070.553441/1‑150 (MQ=60)
                              AATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTC                                                                                                                       >  SRR19973070.623330/1‑150 (MQ=60)
                              AATGTAATCCGCCAGCAGATGGTCGAAATCAGCGCCGCCGAGCGCGGAATCACCGCGGGTTGCCAGCACTACAACCCCGCCGCGAATTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTC                                                                                                                       <  SRR19973070.3142556/150‑1 (MQ=60)
                               ATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCC                                                                                                                      >  SRR19973070.1194445/1‑150 (MQ=60)
                                TGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCT                                                                                                                     >  SRR19973070.1832489/1‑150 (MQ=60)
                                TGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCT                                                                                                                     >  SRR19973070.1832330/1‑150 (MQ=60)
                                   AATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGAC                                                                                                                  >  SRR19973070.2591422/1‑150 (MQ=60)
                                         CCAGCATTTGGTCTAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTACAAACACGCCGCGACTTAAGCGCATAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAAT                                                                                                            <  SRR19973070.1232298/150‑1 (MQ=60)
                                          tgctcttcctatctAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTAAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAACCGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATC                                                                                                           <  SRR19973070.2466152/136‑1 (MQ=60)
                                          tgctctccctatctAATCAGCGCCGCCGCGCGCGTAAACCCCGCGGGTTGCCACCACTACAAACCCGCCGCGACTTAAGCGAATATTGCAAATAACAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATC                                                                                                           <  SRR19973070.23034/136‑1 (MQ=59)
                                          CAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATC                                                                                                           <  SRR19973070.213998/150‑1 (MQ=60)
                                           AGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCC                                                                                                          >  SRR19973070.1289832/1‑150 (MQ=60)
                                            GCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTACAAACCCGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCA                                                                                                         <  SRR19973070.3228295/150‑1 (MQ=60)
                                             CAGATGGTCGAAATCATCGCCGCCGAGCGCGTAATCCCCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAG                                                                                                        <  SRR19973070.2882028/150‑1 (MQ=60)
                                                 TGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCG                                                                                                    >  SRR19973070.1291390/1‑150 (MQ=60)
                                                     CTAAATCATCGCCGCCGAGCGCGTAATCACCGCCGGTTGCCAGCACTACAAACACGCCGCGACTTAAGCGCAGATTGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGG                                                                                                <  SRR19973070.2551902/150‑1 (MQ=60)
                                                      GAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGC                                                                                               >  SRR19973070.743058/1‑150 (MQ=60)
                                                      GAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGC                                                                                               >  SRR19973070.743164/1‑150 (MQ=60)
                                                       AAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCG                                                                                              >  SRR19973070.2333431/1‑150 (MQ=60)
                                                       AAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCG                                                                                              >  SRR19973070.1611953/1‑150 (MQ=60)
                                                       AAATCAGCGCCGCCGAGCGCGGAAACACCGCGGGTTGCCACCAATACAAACACCCCGCGACTTAAGCGAAGAATGTAAATAACAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCG                                                                                              <  SRR19973070.2264054/150‑1 (MQ=60)
                                                        AATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGA                                                                                             >  SRR19973070.1543019/1‑150 (MQ=60)
                                                         ATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGAT                                                                                            >  SRR19973070.3201498/1‑150 (MQ=60)
                                                         ATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGAT                                                                                            >  SRR19973070.3202399/1‑150 (MQ=60)
                                                         ATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGAT                                                                                            >  SRR19973070.3202414/1‑150 (MQ=60)
                                                          tcagcgcggcggtGCGCGGAATCACCGCGGTTTCCAAGCACTCAAAACCCGCCGCGATTTACGCGCAGATTGTAAATATAAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATA                                                                                           <  SRR19973070.1411493/137‑1 (MQ=60)
                                                           CATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCACACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAG                                                                                          <  SRR19973070.1850180/150‑1 (MQ=60)
                                                           CATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAG                                                                                          >  SRR19973070.1796824/1‑150 (MQ=60)
                                                           CATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAG                                                                                          <  SRR19973070.1850317/150‑1 (MQ=60)
                                                           CAGCGCCGCCGAGCGCGGAACCCCCGCCGGTTGCCAGCACTTAAAACACGCCGCGACTAAAGCGCAGAATGGAAATAACAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAG                                                                                          <  SRR19973070.2793278/150‑1 (MQ=60)
                                                            ATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGC                                                                                         >  SRR19973070.1606189/1‑150 (MQ=60)
                                                                    CGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGG                                                                                 >  SRR19973070.2333398/1‑150 (MQ=60)
                                                                    CGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGG                                                                                 >  SRR19973070.2333390/1‑150 (MQ=60)
                                                                         GCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGT                                                                            >  SRR19973070.1859402/1‑150 (MQ=60)
                                                                         GCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGT                                                                            >  SRR19973070.1857991/1‑150 (MQ=60)
                                                                           GGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTC                                                                          >  SRR19973070.782848/1‑150 (MQ=60)
                                                                             AATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGT                                                                        >  SRR19973070.2055188/1‑150 (MQ=60)
                                                                             AATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGT                                                                        >  SRR19973070.2267099/1‑150 (MQ=60)
                                                                             AATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGT                                                                        >  SRR19973070.378754/1‑150 (MQ=60)
                                                                             AATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGT                                                                        >  SRR19973070.2833200/1‑150 (MQ=60)
                                                                                CACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAA                                                                     >  SRR19973070.2203423/1‑150 (MQ=60)
                                                                                  CCGCCGGTTGCCAGCACTACAAACCCGCCGCGACTTAAGCGCAGAATGTAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGT                                                                   <  SRR19973070.1727435/150‑1 (MQ=60)
                                                                                   cgcggtttgccagaaatacaaacccgccgctatTTAAGCGCATAATGTAAATATCACACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTA                                                                  <  SRR19973070.2795028/117‑1 (MQ=60)
                                                                                   CGCCGGTTGCCAGAACTACAAACACGCCGCGACTTAAGCGCATAATGTAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTA                                                                  <  SRR19973070.2794641/150‑1 (MQ=60)
                                                                                    GCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAA                                                                 <  SRR19973070.2398048/150‑1 (MQ=60)
                                                                                     CCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAG                                                                <  SRR19973070.2691282/150‑1 (MQ=60)
                                                                                        GTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGC                                                             >  SRR19973070.3275115/1‑150 (MQ=60)
                                                                                        GTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGC                                                             >  SRR19973070.3275214/1‑150 (MQ=60)
                                                                                        GTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGC                                                             >  SRR19973070.3275790/1‑150 (MQ=60)
                                                                                        GTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGC                                                             >  SRR19973070.3275814/1‑150 (MQ=60)
                                                                                          ggccagaactaaaaaccCGCCGCGAATTAAGCGCAGATTGTAAATAACAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAG                                                           <  SRR19973070.35191/133‑1 (MQ=60)
                                                                                          TGCCAGCACTTCAACCACGCCGCGACTTACGCGCATATTGGAAATAACACCCGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAG                                                           <  SRR19973070.35227/150‑1 (MQ=60)
                                                                                          TGCCACCACTTCAACCACGCCGCGACTTACGCGCATAATGCAAATATCAAACGTCCCTCCACCGAGGTCATACACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAG                                                           <  SRR19973070.35214/150‑1 (MQ=60)
                                                                                             CAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGAC                                                        <  SRR19973070.3294278/150‑1 (MQ=60)
                                                                                             CAGAACTTCAAACACGCCGCTACTTAAGCGCAGAATGGAATTATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGAC                                                        <  SRR19973070.1646386/150‑1 (MQ=60)
                                                                                                  CTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAA                                                   >  SRR19973070.2903623/1‑150 (MQ=60)
                                                                                                   TACAAACCCGCCGCGACTTAAGCGCAGATTGGAAATAACAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAG                                                  <  SRR19973070.1138297/150‑1 (MQ=60)
                                                                                                    TCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGG                                                 >  SRR19973070.647039/1‑150 (MQ=60)
                                                                                                     CAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGC                                                >  SRR19973070.1199040/1‑150 (MQ=60)
                                                                                                     CAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGC                                                >  SRR19973070.1198706/1‑150 (MQ=60)
                                                                                                      AAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCC                                               >  SRR19973070.334505/1‑150 (MQ=60)
                                                                                                       AACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCC                                              >  SRR19973070.2560611/1‑150 (MQ=60)
                                                                                                       AACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCC                                              >  SRR19973070.2560748/1‑150 (MQ=60)
                                                                                                                  ACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGC                                   >  SRR19973070.511072/1‑150 (MQ=60)
                                                                                                                     TAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGC                                >  SRR19973070.924625/1‑150 (MQ=60)
                                                                                                                     TAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGC                                >  SRR19973070.924302/1‑150 (MQ=60)
                                                                                                                       AGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGT                              >  SRR19973070.1906777/1‑150 (MQ=60)
                                                                                                                       AGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGT                              >  SRR19973070.2997782/1‑150 (MQ=60)
                                                                                                                         CGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCT                            <  SRR19973070.2603506/150‑1 (MQ=60)
                                                                                                                           CAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTT                          >  SRR19973070.1311336/1‑150 (MQ=60)
                                                                                                                           CAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTT                          >  SRR19973070.1311052/1‑150 (MQ=60)
                                                                                                                           CAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTT                          >  SRR19973070.2700641/1‑150 (MQ=60)
                                                                                                                           CAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTT                          >  SRR19973070.2700580/1‑150 (MQ=60)
                                                                                                                             GAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGG                        >  SRR19973070.2465366/1‑150 (MQ=60)
                                                                                                                             GAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTGTGG                        >  SRR19973070.3084974/1‑150 (MQ=60)
                                                                                                                                  GAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCC                   >  SRR19973070.109251/1‑150 (MQ=60)
                                                                                                                                   AAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCT                  >  SRR19973070.2614865/1‑150 (MQ=60)
                                                                                                                                   AAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCT                  >  SRR19973070.1614243/1‑150 (MQ=60)
                                                                                                                                       ATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTGACG              <  SRR19973070.3003318/150‑1 (MQ=60)
                                                                                                                                           AACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTGACGCTGG          >  SRR19973070.29343/1‑150 (MQ=60)
                                                                                                                                           AACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTGACGCTGG          >  SRR19973070.1762340/1‑150 (MQ=60)
                                                                                                                                           AACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGGTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCGTTGGTGCCCTGACGCTGG          >  SRR19973070.590115/1‑150 (MQ=60)
                                                                                                                                                  CTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTGACGCTGGGCATCGT   >  SRR19973070.2042515/1‑150 (MQ=60)
                                                                                                                                                  CTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTGACGCTGGGCATCGT   >  SRR19973070.2042465/1‑150 (MQ=60)
                                                                                                                                                   TCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTGACGCTGGGCATCGTC  >  SRR19973070.1601753/1‑150 (MQ=60)
                                                                                                                                                   |                                                                                                                                                     
GCTACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCGCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTGACGCTGGGCATCGTC  >  CP009273/2651518‑2651814

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑