Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,651,665 G→T 100% G210G (GGC→GGA)  hscA ← DnaK‑like molecular chaperone specific for IscU

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,651,6650GT100.0% 601.3 / NA 188G210G (GGC→GGA) hscADnaK‑like molecular chaperone specific for IscU
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (100/88);  total (100/88)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 188 total aligned reads displayed.

ACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCGCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTGACGCTG  >  CP009273/2651521‑2651805
                                                                                                                                                |                                                                                                                                            
aCGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCa                                                                                                                                           <  1:2887336/148‑1 (MQ=255)
 cGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCAc                                                                                                                                          >  2:1994712/1‑148 (MQ=255)
   aTCAGTAATGCCCGCCTGCTCGCTAATGTAATCCGCCAGCATATGGTCGAAATAATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGAACTTCAAACACGCCGCGAATTAAGCGCATAATGGAAATATCAAACGTCCCTCCACCg                                                                                                                                        <  1:722957/148‑1 (MQ=255)
   aTCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCg                                                                                                                                        <  2:791790/148‑1 (MQ=255)
    tCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGACTCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGa                                                                                                                                       >  1:405982/1‑148 (MQ=255)
    tCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGa                                                                                                                                       >  1:2119750/1‑148 (MQ=255)
            gCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGTTGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATaa                                                                                                                               <  2:3386784/148‑1 (MQ=255)
            gCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATaa                                                                                                                               <  1:153182/148‑1 (MQ=255)
             cccGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATaaa                                                                                                                              >  2:2781316/1‑148 (MQ=255)
                   tGCTCGCGATTGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGCGCGCGTAATCACCGCCGGTTGCCAGCACTTAAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGa                                                                                                                        <  2:918699/148‑1 (MQ=255)
                   tGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGa                                                                                                                        >  2:1052880/1‑148 (MQ=255)
                    gCTCGCTATTGTAAACCGCCAGCATTTGTTCTAAATCATCGCCGCCGAGCGCGGAATCACCGCGGGGTGCCAGAACTTAAAACCCGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGAt                                                                                                                       <  1:2188604/148‑1 (MQ=255)
                    gCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGAt                                                                                                                       >  2:1142570/1‑148 (MQ=255)
                    gCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGAt                                                                                                                       <  2:2082260/148‑1 (MQ=255)
                       cgcgAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCAc                                                                                                                    >  1:2733434/1‑148 (MQ=255)
                                aaTCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTg                                                                                                           >  1:1402717/1‑148 (MQ=255)
                                 aTCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGa                                                                                                          >  1:2062343/1‑148 (MQ=255)
                                      ccAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGa                                                                                                     >  1:991364/1‑148 (MQ=255)
                                       cagcagATGGTCTAAATAATCGCCGCCGAGCGCGTAATCACCGCCTGTTGCCAGAACTTCAAACACGCCGCGACTTAAGCGCAGATTGGAAATATAAAACGTCCCTCCACCGAGGTAATAAACGGCGATCACGCCTTCCTGACCGGaa                                                                                                    <  1:2270628/148‑1 (MQ=255)
                                        agcagATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAAt                                                                                                   >  1:1565855/1‑148 (MQ=255)
                                        agcagATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAAt                                                                                                   <  2:2360554/148‑1 (MQ=255)
                                        agcagATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAAt                                                                                                   <  2:2695115/148‑1 (MQ=255)
                                        agcagATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAAt                                                                                                   <  2:2774563/148‑1 (MQ=255)
                                          cagATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATcc                                                                                                 <  2:2684612/148‑1 (MQ=255)
                                          cagATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATcc                                                                                                 >  1:266722/1‑148 (MQ=255)
                                            gATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAg                                                                                               >  1:2781205/1‑148 (MQ=255)
                                             aTGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACTTCCCTCCACCGAGGTCATAAACGTCGATCACGCCTTCCTGACCGGAATCCAGc                                                                                              >  1:179744/1‑148 (MQ=255)
                                             aTGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGc                                                                                              >  1:3405063/1‑148 (MQ=255)
                                                gTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCg                                                                                           >  1:685978/1‑148 (MQ=255)
                                                  cGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTa                                                                                         <  2:1097881/148‑1 (MQ=255)
                                                    aaaTCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATAACAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAgg                                                                                       <  2:1693730/148‑1 (MQ=255)
                                                    aaaTCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACACCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAgg                                                                                       >  2:333090/1‑148 (MQ=255)
                                                      atcatcGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCg                                                                                     >  2:1227250/1‑148 (MQ=255)
                                                      atcatcGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCg                                                                                     >  2:847418/1‑148 (MQ=255)
                                                      atcatcGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCg                                                                                     >  2:591687/1‑148 (MQ=255)
                                                       tcatcGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGa                                                                                    >  2:1791006/1‑148 (MQ=255)
                                                        catcGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGAt                                                                                   >  1:560529/1‑148 (MQ=255)
                                                        catcGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGAt                                                                                   <  2:591892/148‑1 (MQ=255)
                                                        catcGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGAt                                                                                   <  1:1126676/148‑1 (MQ=255)
                                                         atcGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATa                                                                                  >  2:3353378/1‑148 (MQ=255)
                                                         atcGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATa                                                                                  >  2:1055689/1‑148 (MQ=255)
                                                         atcGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATa                                                                                  <  1:2259037/148‑1 (MQ=255)
                                                           cgccgcCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGc                                                                                <  1:1743270/148‑1 (MQ=255)
                                                           cgccgcCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGc                                                                                <  2:1600294/148‑1 (MQ=255)
                                                                ccgAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAg                                                                           >  2:2571284/1‑148 (MQ=255)
                                                                    gcgcGGAATCACCGCGGGTTGCCAGCACTACAAACACGCCGCGACTTAAGCGCATATTGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGcggt                                                                       <  1:739037/148‑1 (MQ=255)
                                                                    gcgcGGAATCACCGCCGGTGGCCAGCACTACAAACACGCCGCGACTTAAGCGCAGAATGGAAATAACAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGcggt                                                                       <  1:404955/148‑1 (MQ=255)
                                                                       cgGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGcggtcgg                                                                    <  1:2556406/148‑1 (MQ=255)
                                                                       aggAATCACCGCCGTTTGCCAGCACTTCAAACACGCCGCGACTTTAGCGCAGAATGGAAATATCATACGTCACTCCACCGAGGTCATAAACGGCGATCACGCCGTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGcggtcgg                                                                    <  2:2963917/147‑1 (MQ=255)
                                                                        ggAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGcggtcggt                                                                   <  2:2192343/148‑1 (MQ=255)
                                                                           aTCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCg                                                                >  2:1910082/1‑148 (MQ=255)
                                                                            tCACCGCCGGTTGCCAGCACTTCAACCACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGt                                                               <  2:904388/148‑1 (MQ=255)
                                                                            tCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGt                                                               <  1:1478752/148‑1 (MQ=255)
                                                                            tCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGt                                                               <  2:3297994/148‑1 (MQ=255)
                                                                            tCACCGCCGGTTGCCAGCACTACAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGt                                                               <  1:2675077/148‑1 (MQ=255)
                                                                              aCCGCCGGTTGCCCCCACTCCAACCACGCCGCGACTTAACCCAACAATGGAAAAAACACACGGCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTta                                                             <  1:3287852/148‑1 (MQ=255)
                                                                               ccgccgGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTtaa                                                            >  1:2144326/1‑148 (MQ=255)
                                                                               ccgccgGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTtaa                                                            >  1:344588/1‑148 (MQ=255)
                                                                                cgccgGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTtaag                                                           >  1:1986634/1‑148 (MQ=255)
                                                                                cgccgGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTtaag                                                           <  1:1754682/148‑1 (MQ=255)
                                                                                 gccgGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTtaagt                                                          >  1:831305/1‑148 (MQ=255)
                                                                                   cgGGTGCCACCACTCCAAACCCGCCGCGACTTACGCGCATAATGGAAATATCAACCGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTtaagtaa                                                        <  1:665449/148‑1 (MQ=255)
                                                                                    ggTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTtaagtaag                                                       <  2:2654956/148‑1 (MQ=255)
                                                                                      ttGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAgcg                                                     >  1:2044332/1‑148 (MQ=255)
                                                                                       tGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAgcgc                                                    <  2:1778329/148‑1 (MQ=255)
                                                                                       tGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAgcgc                                                    <  2:1778280/148‑1 (MQ=255)
                                                                                         ccAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAg                                                  >  1:2488591/1‑148 (MQ=255)
                                                                                          cAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAgg                                                 <  2:806282/148‑1 (MQ=255)
                                                                                           aGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGa                                                >  1:529997/1‑148 (MQ=255)
                                                                                           aGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGa                                                >  1:194252/1‑148 (MQ=255)
                                                                                             cACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACg                                              >  2:214040/1‑148 (MQ=255)
                                                                                              aCTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGt                                             <  1:2711989/148‑1 (MQ=255)
                                                                                                  cAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAg                                         >  2:2597509/1‑148 (MQ=255)
                                                                                                  cAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAg                                         >  2:2882231/1‑148 (MQ=255)
                                                                                                   aaaCACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAgg                                        <  2:1895173/148‑1 (MQ=255)
                                                                                                    aaCACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGc                                       <  2:1064096/148‑1 (MQ=255)
                                                                                                     acacGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGcc                                      >  2:735957/1‑148 (MQ=255)
                                                                                                        cgccgcGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTGCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACAGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGTCCCGc                                   <  2:2311105/148‑1 (MQ=255)
                                                                                                               aCTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGAcgc                            >  1:2488993/1‑148 (MQ=255)
                                                                                                               aCTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGAcgc                            >  1:3547604/1‑148 (MQ=255)
                                                                                                                cTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGAcgcg                           >  1:2656031/1‑148 (MQ=255)
                                                                                                                 ttAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACgcgc                          <  1:1672158/148‑1 (MQ=255)
                                                                                                                 ttAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACgcgc                          >  2:55907/1‑148 (MQ=255)
                                                                                                                  tAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGcgcc                         >  2:2142175/1‑148 (MQ=255)
                                                                                                                      cgcAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGt                     <  2:3012450/148‑1 (MQ=255)
                                                                                                                        cAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCt                   <  2:2875578/148‑1 (MQ=255)
                                                                                                                        cAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCt                   >  2:510416/1‑148 (MQ=255)
                                                                                                                          gAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCttt                 <  1:591687/148‑1 (MQ=255)
                                                                                                                           aaTGGAAATATCAACCGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTg                <  1:1142570/148‑1 (MQ=255)
                                                                                                                             tGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGt              >  2:368612/1‑148 (MQ=255)
                                                                                                                             tGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGt              >  2:2459781/1‑148 (MQ=255)
                                                                                                                              ggAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGACCGTAGGCGATATCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTg             >  2:74888/1‑148 (MQ=255)
                                                                                                                                aaaTATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGcc           >  2:1380985/1‑148 (MQ=255)
                                                                                                                                aaaTATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGcc           >  1:3104218/1‑148 (MQ=255)
                                                                                                                                  atatCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCt         >  2:1915416/1‑148 (MQ=255)
                                                                                                                                   tatCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTg        >  1:1220347/1‑148 (MQ=255)
                                                                                                                                       aaaCGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTGACGc    <  1:2978406/148‑1 (MQ=255)
                                                                                                                                         aCGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTGACGCTg  <  2:474460/148‑1 (MQ=255)
                                                                                                                                         aCGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTGACGCTg  >  1:2143979/1‑148 (MQ=255)
                                                                                                                                         aCGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGGTAAGTAAGCGCAGGACGTGACGGCCCGCCAGACGCGCCGCGTCGGTGGTGCCCGGAGGCTg  >  1:2532541/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                            
ACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCGCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTGACGCTG  >  CP009273/2651521‑2651805

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCTACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCGCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTGACGCTG  >  CP009273/2651518‑2651805
                                                                                                                                                   |                                                                                                                                            
GCTACGATCTGGACTGCCCGCGTGCTCGCTAATGTAATCCGCCAGCAGTTGGTCGAAATAATCGCCGCCGAGCGCGTAATCACCGCGGGTTGCCAGCACTACAAACACGCCGCGACTTAAGCGCAGATTGGAAATATCAAACGTCCCTCC                                                                                                                                            <  SRR19973066.3019313/150‑1 (MQ=60)
 CTACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCA                                                                                                                                           <  SRR19973066.539306/150‑1 (MQ=60)
   ACGATCAGGAATGCCCGCCTGCTCGCTAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACC                                                                                                                                         <  SRR19973066.2906653/150‑1 (MQ=60)
     GATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGACTCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGA                                                                                                                                       >  SRR19973066.409176/1‑150 (MQ=60)
     GATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGA                                                                                                                                       >  SRR19973066.2167787/1‑150 (MQ=60)
     GATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGA                                                                                                                                       >  SRR19973066.2134404/1‑150 (MQ=60)
      ATCAGTAATGCCCGCCTGCTCGCTAATGTAATCCGCCAGCATATGGTCGAAATAATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGAACTTCAAACACGCCGCGAATTAAGCGCATAATGGAAATATCAAACGTCCCTCCACCGAG                                                                                                                                      <  SRR19973066.728316/150‑1 (MQ=60)
      ATCAGTAATGCCCGCCTGCTCGCGATTTTAATCCGCCAGCTGATGGTCGAAATCATCGCCGCCGCGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAG                                                                                                                                      <  SRR19973066.733426/150‑1 (MQ=60)
       TCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGG                                                                                                                                     <  SRR19973066.2143623/150‑1 (MQ=60)
       TCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGG                                                                                                                                     <  SRR19973066.537985/150‑1 (MQ=60)
        CAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGT                                                                                                                                    >  SRR19973066.1704555/1‑150 (MQ=60)
              TGCCCGCCTGCTCGCTAATGTAATCCGCCAGCTGTTGGTCGAAAACATCGCCGCCGAGCGCGTAATCACCGCGGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAA                                                                                                                              <  SRR19973066.945194/150‑1 (MQ=60)
               GCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAAC                                                                                                                             <  SRR19973066.154610/150‑1 (MQ=60)
                      TGCTCGCTATTGTAATCCGCCAGCTTTTGGTCTAAATCAGCGCCGCCGCGCGCGGAAACCCCGCGGGTGGCCACCAATAAAAACACCCCGCTACTAAAGCGCATATTGTAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATC                                                                                                                      <  SRR19973066.1032353/150‑1 (MQ=19)
                       GCTCGCTATTGTAAACCGCCAGCATTTGTTCTAAATCATCGCCGCCGAGCGCGGAATCACCGCGGGGTGCCAGAACTTAAAACCCGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCA                                                                                                                     <  SRR19973066.2203789/150‑1 (MQ=60)
                        CTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCAC                                                                                                                    >  SRR19973066.2751819/1‑150 (MQ=60)
                           gctattttacTCCGCCAGCTGTTGGTCGAAATCATCGCCGCCGCGCGCGTAATCACCGCCGGTTGCCAGCACTTAAAACACGCCGCGAATTAAGCGAAGATTGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCC                                                                                                                 <  SRR19973066.1563215/140‑1 (MQ=60)
                           GCGATTGTAATCCGCAAGCATTTGGTCTAAATCAGCGCCGCCGCGGGGGTAATCACCGCCGGTTGCCAGAACTTAAACCACGCCGCGACTTACGCGAATATTGTAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCC                                                                                                                 <  SRR19973066.1563196/150‑1 (MQ=45)
                             GAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTT                                                                                                               >  SRR19973066.2072102/1‑150 (MQ=60)
                              AATGTAATCCGCCAGCAGATGGTCTAAATCATCGCCGCCGAGGGCGTAATCACCGCGGGTTGCCAGCACTTCAAACACGCCGCGAATTAAGCGCAGAATGTAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTC                                                                                                              <  SRR19973066.2512974/150‑1 (MQ=60)
                                TGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGTAATCACCGCCGGTTGCCAGCACTACAAACCCGCCGCGACTTAAGCGCAGAATGTAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCT                                                                                                            <  SRR19973066.1671260/150‑1 (MQ=60)
                                 GTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTG                                                                                                           >  SRR19973066.1412816/1‑150 (MQ=60)
                                  TAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGA                                                                                                          >  SRR19973066.2076635/1‑150 (MQ=60)
                                     TCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCG                                                                                                       >  SRR19973066.1952005/1‑150 (MQ=60)
                                       CGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGA                                                                                                     >  SRR19973066.998672/1‑150 (MQ=60)
                                         CCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAAT                                                                                                   >  SRR19973066.1577135/1‑150 (MQ=60)
                                          CAGCAGATGGTCTAAATAATCGCCGCCGAGCGCGTAATCACCGCCTGTTGCCAGAACTTCAAACACGCCGCGACTTAAGCGCAGATTGGAAATATAAAACGTCCCTCCACCGAGGTAATAAACGGCGATCACGCCTTCCTGACCGGAATC                                                                                                  <  SRR19973066.2286237/150‑1 (MQ=60)
                                           AGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCC                                                                                                 >  SRR19973066.268712/1‑150 (MQ=60)
                                             CAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAG                                                                                               >  SRR19973066.2799933/1‑150 (MQ=60)
                                              AGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACTTCCCTCCACCGAGGTCATAAACGTCGATCACGCCTTCCTGACCGGAATCCAGC                                                                                              >  SRR19973066.181320/1‑150 (MQ=60)
                                              AGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGC                                                                                              >  SRR19973066.2023162/1‑150 (MQ=60)
                                              AGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGC                                                                                              >  SRR19973066.3427798/1‑150 (MQ=60)
                                                 TGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCG                                                                                           >  SRR19973066.691018/1‑150 (MQ=60)
                                                 TGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCG                                                                                           >  SRR19973066.690987/1‑150 (MQ=60)
                                                 TGGTCGAAATCATCGCCGCCGAGCGCGGAAACACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGATTGGAAATATCACACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCG                                                                                           <  SRR19973066.212486/150‑1 (MQ=60)
                                                         ATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGAT                                                                                   >  SRR19973066.564532/1‑150 (MQ=60)
                                                           CATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAG                                                                                 <  SRR19973066.1134773/150‑1 (MQ=60)
                                                            ATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACCCGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGC                                                                                <  SRR19973066.2274598/150‑1 (MQ=60)
                                                            ATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGC                                                                                <  SRR19973066.2274600/150‑1 (MQ=60)
                                                            ATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGC                                                                                <  SRR19973066.2273007/150‑1 (MQ=60)
                                                              CGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCG                                                                              <  SRR19973066.1755830/150‑1 (MQ=60)
                                                              CGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCG                                                                              <  SRR19973066.3529462/150‑1 (MQ=60)
                                                                   cggcggggggaatcacggcgggttccaaccactacaaacccgccgcgagttacgcgctgatgGGAAATATCACACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCG                                                                         <  SRR19973066.3021413/88‑1 (MQ=60)
                                                                     GAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGT                                                                       >  SRR19973066.1525192/1‑150 (MQ=60)
                                                                      AGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATCGCCGCAGCGGTC                                                                      >  SRR19973066.2740261/1‑150 (MQ=60)
                                                                       GCGCGTAATCACCGCCGGTTGCCAGCACTTCAAACCCGCCGCGACTAAAGCGCATAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCG                                                                     <  SRR19973066.407491/150‑1 (MQ=60)
                                                                       GCGCGGAATCCCCGCCGGTTGCCACCACTTCAACCCCGCCGCGACTAACGCGCATAATGGAAATATCACACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCG                                                                     <  SRR19973066.408183/150‑1 (MQ=60)
                                                                       GCGCGGAATCACCGCGGGTTGCCAGCACTACAAACACGCCGCGACTTAAGCGCATATTGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCG                                                                     <  SRR19973066.744522/150‑1 (MQ=60)
                                                                       GCGCGGAATCACCGCCGGTGGCCAGCACTACAAACACGCCGCGACTTAAGCGCAGAATGGAAATAACAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCG                                                                     <  SRR19973066.408141/150‑1 (MQ=60)
                                                                          CGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTT                                                                  <  SRR19973066.2573764/150‑1 (MQ=60)
                                                                          CGGAATCACCGCCGGTGGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTT                                                                  <  SRR19973066.2573824/150‑1 (MQ=60)
                                                                             AATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGT                                                               >  SRR19973066.1771499/1‑150 (MQ=60)
                                                                               TCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTA                                                             <  SRR19973066.1489152/150‑1 (MQ=60)
                                                                               TCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTA                                                             >  SRR19973066.3371848/1‑150 (MQ=60)
                                                                               TCACCGCCGGTTGCCAGCACTACAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTA                                                             <  SRR19973066.2692946/150‑1 (MQ=60)
                                                                                CACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAA                                                            >  SRR19973066.347249/1‑150 (MQ=60)
                                                                                CACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAA                                                            >  SRR19973066.347259/1‑150 (MQ=60)
                                                                                CACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAA                                                            >  SRR19973066.2159211/1‑150 (MQ=60)
                                                                                 ACCGCCGGTTGCCCCCACTCCAACCACGCCGCGACTTAACCCAACAATGGAAAAAACACACGGCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAG                                                           <  SRR19973066.3309524/150‑1 (MQ=60)
                                                                                 ACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAG                                                           >  SRR19973066.2000638/1‑150 (MQ=60)
                                                                                 ACCGCCGGTTGCCAGAACTTCAAACCCGCCGCGAATTATGCGCAGAATGTAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAG                                                           <  SRR19973066.2559814/150‑1 (MQ=60)
                                                                                  CCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGT                                                          >  SRR19973066.837366/1‑150 (MQ=60)
                                                                                  CCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGT                                                          <  SRR19973066.1429525/150‑1 (MQ=60)
                                                                                  CCGCCGGTTGCCAGCACTACAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAACCGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGT                                                          <  SRR19973066.2686708/150‑1 (MQ=60)
                                                                                   CGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAACCGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTA                                                         <  SRR19973066.1199073/150‑1 (MQ=60)
                                                                                   CGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTA                                                         >  SRR19973066.641767/1‑150 (MQ=60)
                                                                                   CGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTA                                                         <  SRR19973066.1767296/150‑1 (MQ=60)
                                                                                      CGGGTGCCACCACTCCAAACCCGCCGCGACTTACGCGCATAATGGAAATATCAACCGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGC                                                      <  SRR19973066.670306/150‑1 (MQ=60)
                                                                                       GGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCG                                                     >  SRR19973066.244562/1‑150 (MQ=60)
                                                                                       GGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCG                                                     >  SRR19973066.2058555/1‑150 (MQ=60)
                                                                                          TGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAG                                                  >  SRR19973066.2505591/1‑150 (MQ=60)
                                                                                          TGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAG                                                  >  SRR19973066.2505573/1‑150 (MQ=60)
                                                                                          TGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAG                                                  >  SRR19973066.1976200/1‑150 (MQ=60)
                                                                                            CCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGA                                                >  SRR19973066.195920/1‑150 (MQ=60)
                                                                                            CCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGA                                                >  SRR19973066.533868/1‑150 (MQ=60)
                                                                                                 ACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGA                                           <  SRR19973066.2730199/150‑1 (MQ=60)
                                                                                                 ACTTAAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGA                                           <  SRR19973066.2730107/150‑1 (MQ=60)
                                                                                                       AACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCC                                     >  SRR19973066.3002473/1‑150 (MQ=60)
                                                                                                                CGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGC                            >  SRR19973066.2505977/1‑150 (MQ=60)
                                                                                                                CGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGC                            >  SRR19973066.3571154/1‑150 (MQ=60)
                                                                                                                CGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGC                            >  SRR19973066.704448/1‑150 (MQ=60)
                                                                                                                CGACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGC                            >  SRR19973066.2753004/1‑150 (MQ=60)
                                                                                                                 GACTTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCG                           >  SRR19973066.2673841/1‑150 (MQ=60)
                                                                                                                    TTAAGCGCAGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCG                        <  SRR19973066.1684344/150‑1 (MQ=60)
                                                                                                                            AGAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTG                >  SRR19973066.485207/1‑150 (MQ=60)
                                                                                                                             GAATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGG               <  SRR19973066.595795/150‑1 (MQ=60)
                                                                                                                              AATGGAAATATCAACCGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGT              <  SRR19973066.1150733/150‑1 (MQ=60)
                                                                                                                               ATGGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTG             <  SRR19973066.2842332/150‑1 (MQ=60)
                                                                                                                                 GGAAATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCC           >  SRR19973066.3124950/1‑150 (MQ=60)
                                                                                                                                    AATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTG        >  SRR19973066.1228988/1‑150 (MQ=60)
                                                                                                                                    AATATCAAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTG        >  SRR19973066.1228964/1‑150 (MQ=60)
                                                                                                                                         CACACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTGACGCT   <  SRR19973066.829050/150‑1 (MQ=60)
                                                                                                                                          AAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTGACGCTG  <  SRR19973066.2998084/150‑1 (MQ=60)
                                                                                                                                          AAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTGACGCTG  <  SRR19973066.2997997/150‑1 (MQ=60)
                                                                                                                                          AAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTGACGCTG  >  SRR19973066.2158862/1‑150 (MQ=60)
                                                                                                                                          AAACGTCCCTCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGGTAAGTAAGCGCAGGACGTGACGGCCCGCCAGACGCGCCGCGTCGGTGGTGCCCGGAGGCTG  >  SRR19973066.2549759/1‑150 (MQ=60)
                                                                                                                                                   |                                                                                                                                            
GCTACGATCAGGAATGCCCGCCTGCTCGCGAATGTAATCCGCCAGCAGATGGTCGAAATCATCGCCGCCGAGCGCGGAATCACCGCCGGTTGCCAGCACTTCAAACACGCCGCGACTTAAGCGCAGAATGGAAATATCAAACGTCCCGCCACCGAGGTCATAAACGGCGATCACGCCTTCCTGACCGGAATCCAGCCCGTAGGCGATAGCCGCAGCGGTCGGTTCGTTAAGTAAGCGCAGGACGTGAAGGCCCGCCAGACGCGCCGCGTCTTTGGTGCCCTGACGCTG  >  CP009273/2651518‑2651805

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑