Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,659,594 C→A 91.7% L267L (CTC→CTA)  csiE → stationary phase inducible protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,659,5940CA91.7% 485.1 / 37.4 180L267L (CTC→CTA) csiEstationary phase inducible protein
Reads supporting (aligned to +/- strand):  ref base C (7/8);  new base A (92/73);  total (99/81)
Fisher's exact test for biased strand distribution p-value = 5.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.37e-01

Only 100 of 180 total aligned reads displayed.

GGTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCTTTCTGA  >  CP009273/2659450‑2659740
                                                                                                                                                |                                                                                                                                                  
ggTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGcc                                                                                                                                                 <  1:2159550/148‑1 (MQ=255)
      cGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCg                                                                                                                                           >  2:737179/1‑148 (MQ=255)
       gATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGt                                                                                                                                          >  2:2444481/1‑148 (MQ=255)
        aTCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGtt                                                                                                                                         <  2:3009304/148‑1 (MQ=255)
        aTCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGtt                                                                                                                                         <  2:3009353/148‑1 (MQ=255)
        aTCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGtt                                                                                                                                         >  2:596167/1‑148 (MQ=255)
          ccATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTCGCCCAGGCGTTaa                                                                                                                                       <  1:387149/148‑1 (MQ=255)
            aTTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAAt                                                                                                                                     >  1:1061031/1‑148 (MQ=255)
             ttGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATc                                                                                                                                    >  2:1515957/1‑148 (MQ=255)
                cGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCa                                                                                                                                 >  2:2624028/1‑148 (MQ=255)
                  ggATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAgt                                                                                                                               >  1:1478826/1‑148 (MQ=255)
                  ggATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAgt                                                                                                                               <  2:245847/148‑1 (MQ=255)
                  ggATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAgt                                                                                                                               <  1:2568315/148‑1 (MQ=255)
                  ggATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAgt                                                                                                                               <  2:20132/148‑1 (MQ=255)
                   gATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAgtt                                                                                                                              <  1:2613726/148‑1 (MQ=255)
                    aTGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAgttt                                                                                                                             <  1:1622476/148‑1 (MQ=255)
                      gcgcATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAgtttgt                                                                                                                           <  2:2647909/148‑1 (MQ=255)
                       cgcATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAgtttgtt                                                                                                                          >  1:254406/1‑148 (MQ=255)
                         cATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGtttgtttg                                                                                                                        <  1:2965361/148‑1 (MQ=255)
                         cATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGtttgtttg                                                                                                                        >  2:1081428/1‑148 (MQ=255)
                          aTCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGc                                                                                                                       <  2:1379541/148‑1 (MQ=255)
                            cAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCa                                                                                                                     >  1:1473535/1‑148 (MQ=255)
                             aGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCAt                                                                                                                    <  2:712417/148‑1 (MQ=255)
                             aGCGGGACAGACAAGTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCAt                                                                                                                    <  2:710882/148‑1 (MQ=255)
                               cGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCg                                                                                                                  >  1:2338667/1‑148 (MQ=255)
                                 ggACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGt                                                                                                                >  1:2511927/1‑148 (MQ=255)
                                 ggACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGt                                                                                                                <  2:2431964/148‑1 (MQ=255)
                                 ggACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGt                                                                                                                <  2:2582139/148‑1 (MQ=255)
                                  gacagacaATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTa                                                                                                               >  1:1927334/1‑148 (MQ=255)
                                   acagacaATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTAt                                                                                                              >  2:1783951/1‑148 (MQ=255)
                                    cagacaATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTAtt                                                                                                             <  1:677680/148‑1 (MQ=255)
                                     agacaATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTg                                                                                                            <  2:3268650/148‑1 (MQ=255)
                                       acaATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGat                                                                                                          <  1:21742/148‑1 (MQ=255)
                                       acaATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGat                                                                                                          <  1:21070/148‑1 (MQ=255)
                                       acaATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGat                                                                                                          <  2:1820675/148‑1 (MQ=255)
                                        caATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGata                                                                                                         >  2:2264149/1‑148 (MQ=255)
                                        caATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGata                                                                                                         >  2:285547/1‑148 (MQ=255)
                                        caATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGata                                                                                                         <  1:1515957/148‑1 (MQ=255)
                                          aTTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGataat                                                                                                       <  1:1105418/148‑1 (MQ=255)
                                          aTTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGataat                                                                                                       >  2:1293833/1‑148 (MQ=255)
                                           ttGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGataata                                                                                                      >  2:1471682/1‑148 (MQ=255)
                                             gCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATAcc                                                                                                    <  2:2247338/148‑1 (MQ=255)
                                             gCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATAcc                                                                                                    <  1:2229428/148‑1 (MQ=255)
                                                tCAGTCTATCAAACGTCTGGTAAACCATTCTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTg                                                                                                 <  1:776404/148‑1 (MQ=255)
                                                 cAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGc                                                                                                >  1:412122/1‑148 (MQ=255)
                                                   gTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCg                                                                                              >  2:2454959/1‑148 (MQ=255)
                                                       aTCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAag                                                                                          >  1:1094841/1‑148 (MQ=255)
                                                          aaaCGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGt                                                                                       >  2:2882432/1‑148 (MQ=255)
                                                           aaCGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGtt                                                                                      >  2:351362/1‑148 (MQ=255)
                                                           aaCGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGtt                                                                                      >  2:3093554/1‑148 (MQ=255)
                                                           aaCGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGtt                                                                                      >  1:3001771/1‑148 (MQ=255)
                                                            aCGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTc                                                                                     >  1:234138/1‑148 (MQ=255)
                                                            aCGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTc                                                                                     >  1:2214881/1‑148 (MQ=255)
                                                            aCGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTc                                                                                     >  1:2826332/1‑148 (MQ=255)
                                                               tCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGcc                                                                                  <  2:2203189/148‑1 (MQ=255)
                                                                cTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCa                                                                                 >  1:2393752/1‑148 (MQ=255)
                                                                  ggTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGa                                                                               >  1:462707/1‑148 (MQ=255)
                                                                  ggTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGa                                                                               >  1:2626706/1‑148 (MQ=255)
                                                                     aaaCCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTg                                                                            >  2:2746417/1‑148 (MQ=255)
                                                                     aaaCCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTg                                                                            >  2:2746398/1‑148 (MQ=255)
                                                                      aaCCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGt                                                                           >  1:2655131/1‑148 (MQ=255)
                                                                         cATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTAcc                                                                        >  2:509018/1‑148 (MQ=255)
                                                                            tttCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCAc                                                                     >  2:1341652/1‑148 (MQ=255)
                                                                              tCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGc                                                                   <  2:21874/148‑1 (MQ=255)
                                                                                  gAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTgg                                                               >  1:1044296/1‑148 (MQ=255)
                                                                                   aGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGACGAGTTCGCCAGACTGTACCCACGCCTGGt                                                              >  2:1911869/1‑148 (MQ=255)
                                                                                    gCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTg                                                             >  1:100849/1‑148 (MQ=255)
                                                                                    gCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTg                                                             <  2:2531893/148‑1 (MQ=255)
                                                                                           aaTGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGcacca                                                      >  1:784738/1‑148 (MQ=255)
                                                                                                   tttttATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCcgcgcg                                              >  1:229839/1‑148 (MQ=255)
                                                                                                    ttttATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCgcgg                                             >  1:904474/1‑148 (MQ=255)
                                                                                                      ttATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCgcggcg                                           <  2:1061031/148‑1 (MQ=255)
                                                                                                      ttATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCgcggcg                                           >  2:2375455/1‑148 (MQ=255)
                                                                                                      ttATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCgcggcg                                           <  1:204619/148‑1 (MQ=255)
                                                                                                      ttATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCgcggcg                                           <  1:205509/148‑1 (MQ=255)
                                                                                                      ttATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCgcggcg                                           <  1:1274813/148‑1 (MQ=255)
                                                                                                        atgatgAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCgcgctgcgct                                         <  2:152866/148‑1 (MQ=255)
                                                                                                        atgatgAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCgcggcgat                                         <  1:328865/148‑3 (MQ=255)
                                                                                                         tgatgaACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTg                                        >  1:2253569/1‑148 (MQ=255)
                                                                                                           atgaACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGc                                      <  1:1722510/148‑1 (MQ=255)
                                                                                                              aaCAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCgg                                   <  2:1478826/148‑1 (MQ=255)
                                                                                                              aaCAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCgg                                   >  1:3111532/1‑148 (MQ=255)
                                                                                                               aCAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGa                                  <  1:596167/148‑1 (MQ=255)
                                                                                                                  ggggTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGAttt                               >  1:950537/1‑148 (MQ=255)
                                                                                                                    ggTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCAGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGa                             >  1:2316233/1‑148 (MQ=255)
                                                                                                                      ttATGTGATCAGCTTTATACCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGaaa                           <  1:539925/148‑1 (MQ=255)
                                                                                                                       tATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAg                          <  1:285547/148‑1 (MQ=255)
                                                                                                                          gtgATCAGCTTTATACCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGa                       <  1:1000384/148‑1 (MQ=255)
                                                                                                                           tgATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGaa                      >  1:792294/1‑148 (MQ=255)
                                                                                                                           tgATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGaa                      >  1:1856633/1‑148 (MQ=255)
                                                                                                                            gATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAAt                     <  1:3068751/148‑1 (MQ=255)
                                                                                                                                 gCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGc                >  2:2134020/1‑148 (MQ=255)
                                                                                                                                  cTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCg               <  2:2662162/148‑1 (MQ=255)
                                                                                                                                   tttATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGt              <  2:2393752/148‑1 (MQ=255)
                                                                                                                                        aCCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATc         >  2:2774798/1‑148 (MQ=255)
                                                                                                                                         cccACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCt        <  1:1709498/148‑1 (MQ=255)
                                                                                                                                         cccACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCt        <  1:2264155/148‑1 (MQ=255)
                                                                                                                                           cACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCttt      <  2:2511927/148‑1 (MQ=255)
                                                                                                                                           cACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCttt      >  1:2738851/1‑148 (MQ=255)
                                                                                                                                           cACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCttt      >  1:3230564/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
GGTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCTTTCTGA  >  CP009273/2659450‑2659740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCTTTCTGA  >  CP009273/2659450‑2659740
                                                                                                                                                |                                                                                                                                                  
GGTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCA                                                                                                                                               <  SRR19973070.2173906/150‑1 (MQ=60)
GGTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCA                                                                                                                                               <  SRR19973070.2173509/150‑1 (MQ=60)
     CCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGT                                                                                                                                          >  SRR19973070.1064543/1‑150 (MQ=60)
         TCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAA                                                                                                                                      >  SRR19973070.571080/1‑150 (MQ=60)
          CCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTCGCCCAGGCGTTAAAT                                                                                                                                     <  SRR19973070.389224/150‑1 (MQ=60)
          CCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTCGCCCAGGCGTTAAAT                                                                                                                                     <  SRR19973070.389986/150‑1 (MQ=60)
          CCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAAT                                                                                                                                     >  SRR19973070.1068470/1‑150 (MQ=60)
           CATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATC                                                                                                                                    >  SRR19973070.1366593/1‑150 (MQ=60)
                CGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGT                                                                                                                               >  SRR19973070.1489617/1‑150 (MQ=60)
                CGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGT                                                                                                                               >  SRR19973070.1489176/1‑150 (MQ=60)
                  GGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTT                                                                                                                             <  SRR19973070.2585118/150‑1 (MQ=60)
                  GGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTT                                                                                                                             <  SRR19973070.2585100/150‑1 (MQ=60)
                  GGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTT                                                                                                                             <  SRR19973070.1048068/150‑1 (MQ=60)
                   GATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTG                                                                                                                            <  SRR19973070.2630781/150‑1 (MQ=60)
                   GATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTG                                                                                                                            <  SRR19973070.2630833/150‑1 (MQ=60)
                    ATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGT                                                                                                                           <  SRR19973070.1633751/150‑1 (MQ=60)
                     TGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTT                                                                                                                          >  SRR19973070.256129/1‑150 (MQ=60)
                        GCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGC                                                                                                                       <  SRR19973070.955197/150‑1 (MQ=60)
                         CATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCC                                                                                                                      <  SRR19973070.2984425/150‑1 (MQ=60)
                          ATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCA                                                                                                                     >  SRR19973070.1483989/1‑150 (MQ=60)
                          ATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGGTTGTTTGCCA                                                                                                                     >  SRR19973070.1483841/1‑150 (MQ=60)
                             AGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCG                                                                                                                  >  SRR19973070.2517536/1‑150 (MQ=60)
                             AGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCG                                                                                                                  >  SRR19973070.2354189/1‑150 (MQ=60)
                               CGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGT                                                                                                                >  SRR19973070.2528280/1‑150 (MQ=60)
                                GGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTA                                                                                                               >  SRR19973070.2366074/1‑150 (MQ=60)
                                GGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTA                                                                                                               >  SRR19973070.1940322/1‑150 (MQ=60)
                                   ACAGACAATTGCGTCATTATATCATTTGTCTGGTAAACCATTTTCGTGATCTGGGTAATGTTAGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCATTTTGTTTGCCATCGGTATTG                                                                                                            <  SRR19973070.1554733/150‑1 (MQ=60)
                                    CAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGA                                                                                                           <  SRR19973070.682513/150‑1 (MQ=60)
                                    CAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGA                                                                                                           <  SRR19973070.2354442/150‑1 (MQ=60)
                                       ACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAA                                                                                                        <  SRR19973070.22063/150‑1 (MQ=60)
                                       ACAATCGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAA                                                                                                        <  SRR19973070.21375/150‑1 (MQ=60)
                                        CACTTGCGTCAGTATATCAACCGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGATTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCTGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAAT                                                                                                       <  SRR19973070.1773420/150‑1 (MQ=60)
                                        CAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAAT                                                                                                       <  SRR19973070.1526584/150‑1 (MQ=60)
                                          ATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATAC                                                                                                     <  SRR19973070.1112998/150‑1 (MQ=60)
                                             GCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCT                                                                                                  <  SRR19973070.533813/150‑1 (MQ=60)
                                             GCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCT                                                                                                  <  SRR19973070.2244100/150‑1 (MQ=60)
                                              CGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTG                                                                                                 >  SRR19973070.1098313/1‑150 (MQ=60)
                                               GTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGC                                                                                                >  SRR19973070.415104/1‑150 (MQ=60)
                                               GTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGC                                                                                                >  SRR19973070.415129/1‑150 (MQ=60)
                                                TCAGTCTATCAAACGTCTGGTAAACCATTCTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCC                                                                                               <  SRR19973070.781836/150‑1 (MQ=60)
                                                     CTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAG                                                                                          >  SRR19973070.1102393/1‑150 (MQ=60)
                                                         CAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTT                                                                                      >  SRR19973070.3020969/1‑150 (MQ=60)
                                                         CAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTT                                                                                      >  SRR19973070.3020947/1‑150 (MQ=60)
                                                          AAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTC                                                                                     >  SRR19973070.2844622/1‑150 (MQ=60)
                                                          AAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTC                                                                                     >  SRR19973070.235783/1‑150 (MQ=60)
                                                          AAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTC                                                                                     >  SRR19973070.2229435/1‑150 (MQ=60)
                                                             CGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCC                                                                                  >  SRR19973070.3024866/1‑150 (MQ=60)
                                                              GTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCA                                                                                 >  SRR19973070.739542/1‑150 (MQ=60)
                                                              GTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCA                                                                                 >  SRR19973070.2409553/1‑150 (MQ=60)
                                                                CTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGA                                                                               >  SRR19973070.2643840/1‑150 (MQ=60)
                                                                CTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGA                                                                               >  SRR19973070.466004/1‑150 (MQ=60)
                                                                    TAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGT                                                                           >  SRR19973070.2672403/1‑150 (MQ=60)
                                                                          ATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCAC                                                                     <  SRR19973070.1109558/150‑1 (MQ=60)
                                                                          ATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCAC                                                                     <  SRR19973070.2770258/150‑1 (MQ=60)
                                                                           TTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACG                                                                    >  SRR19973070.2080191/1‑150 (MQ=60)
                                                                                GTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGG                                                               >  SRR19973070.1051659/1‑150 (MQ=60)
                                                                                  GAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCTCCAGACTGTACCCACGCCTGGTG                                                             >  SRR19973070.229689/1‑150 (MQ=60)
                                                                                  GAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTG                                                             >  SRR19973070.101814/1‑150 (MQ=60)
                                                                                         GAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCA                                                      >  SRR19973070.790202/1‑150 (MQ=60)
                                                                                            ATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCC                                                   <  SRR19973070.2183102/150‑1 (MQ=60)
                                                                                                 CGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCG                                              >  SRR19973070.231495/1‑150 (MQ=60)
                                                                                                 CGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCG                                              >  SRR19973070.231465/1‑150 (MQ=60)
                                                                                                  GTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGG                                             >  SRR19973070.910893/1‑150 (MQ=60)
                                                                                                      TTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCT                                         <  SRR19973070.1283729/150‑1 (MQ=60)
                                                                                                      TTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCT                                         <  SRR19973070.207049/150‑1 (MQ=60)
                                                                                                      TTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCT                                         <  SRR19973070.206157/150‑1 (MQ=60)
                                                                                                       TATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTG                                        >  SRR19973070.2268507/1‑150 (MQ=60)
                                                                                                        ATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGATGG                                       <  SRR19973070.330397/150‑1 (MQ=60)
                                                                                                        ATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGATGG                                       <  SRR19973070.331271/150‑1 (MQ=60)
                                                                                                         TGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGC                                      >  SRR19973070.2024193/1‑150 (MQ=60)
                                                                                                           ATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCG                                    <  SRR19973070.1734435/150‑1 (MQ=60)
                                                                                                            TGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGG                                   >  SRR19973070.3131606/1‑150 (MQ=60)
                                                                                                             GAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCATCACCCGCGCGGCGCTGGCCGGA                                  >  SRR19973070.2263054/1‑150 (MQ=60)
                                                                                                             GAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCATCACCCGCGCGGCGCTGGCCGGA                                  >  SRR19973070.2536162/1‑150 (MQ=60)
                                                                                                             GAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGA                                  >  SRR19973070.804551/1‑150 (MQ=60)
                                                                                                               ACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATT                                <  SRR19973070.600307/150‑1 (MQ=60)
                                                                                                                CAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTT                               >  SRR19973070.957312/1‑150 (MQ=60)
                                                                                                                  GGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCAGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGA                             >  SRR19973070.2331622/1‑150 (MQ=60)
                                                                                                                      TTATGTGATCAGCTTTATACCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGT                         <  SRR19973070.543535/150‑1 (MQ=60)
                                                                                                                      TTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCGGGCCGGATTTGAAAGT                         <  SRR19973070.314396/150‑1 (MQ=60)
                                                                                                                       TATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTG                        <  SRR19973070.287460/150‑1 (MQ=60)
                                                                                                                       TATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTG                        <  SRR19973070.287454/150‑1 (MQ=60)
                                                                                                                         TGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAA                      >  SRR19973070.1869493/1‑150 (MQ=60)
                                                                                                                         TGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAA                      >  SRR19973070.1869348/1‑150 (MQ=60)
                                                                                                                         TGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAA                      >  SRR19973070.797777/1‑150 (MQ=60)
                                                                                                                         TGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAA                      >  SRR19973070.797448/1‑150 (MQ=60)
                                                                                                                          GTGATCAGCTTTATACCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAAT                     <  SRR19973070.1007513/150‑1 (MQ=60)
                                                                                                                          GAGATCAGCTTTATACCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAAT                     <  SRR19973070.2671627/150‑1 (MQ=60)
                                                                                                                            GATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATAC                   <  SRR19973070.3088438/150‑1 (MQ=60)
                                                                                                                            GATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATAC                   >  SRR19973070.2854472/1‑150 (MQ=60)
                                                                                                                                  CTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTC             >  SRR19973070.2212547/1‑150 (MQ=60)
                                                                                                                                         CCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCTTT      >  SRR19973070.2756478/1‑150 (MQ=60)
                                                                                                                                         CCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCTTT      >  SRR19973070.3251428/1‑150 (MQ=60)
                                                                                                                                         CCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCTTT      <  SRR19973070.2279179/150‑1 (MQ=60)
                                                                                                                                         CCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCTTT      <  SRR19973070.2279173/150‑1 (MQ=60)
                                                                                                                                         CCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCTTT      <  SRR19973070.1721303/150‑1 (MQ=60)
                                                                                                                                         CCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCTTT      <  SRR19973070.606324/150‑1 (MQ=60)
                                                                                                                                         CCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAAGACGGCGTCCATCTTT      <  SRR19973070.1721343/150‑1 (MQ=60)
                                                                                                                                             CCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCTTTCTGA  >  SRR19973070.1600526/1‑150 (MQ=60)
                                                                                                                                                |                                                                                                                                                  
GGTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCTTTCTGA  >  CP009273/2659450‑2659740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑