Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,659,594 C→A 100% L267L (CTC→CTA)  csiE → stationary phase inducible protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,659,5940CA97.7% 676.8 / 5.8 217L267L (CTC→CTA) csiEstationary phase inducible protein
Reads supporting (aligned to +/- strand):  ref base C (0/5);  new base A (117/95);  total (117/100)
Fisher's exact test for biased strand distribution p-value = 1.97e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 217 total aligned reads displayed.

GGGTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCTTTCT  >  CP009273/2659449‑2659738
                                                                                                                                                 |                                                                                                                                                
 ggTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGcc                                                                                                                                               <  2:2216150/148‑1 (MQ=255)
 ggTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGcc                                                                                                                                               <  2:426620/148‑1 (MQ=255)
   ttCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCa                                                                                                                                             <  2:1454182/148‑1 (MQ=255)
    tCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAg                                                                                                                                            >  1:1173513/1‑148 (MQ=255)
    tCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAg                                                                                                                                            <  1:1050413/148‑1 (MQ=255)
          tCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTa                                                                                                                                      >  1:782283/1‑148 (MQ=255)
          tCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTa                                                                                                                                      >  2:3217901/1‑148 (MQ=255)
          tCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTa                                                                                                                                      >  2:1248547/1‑148 (MQ=255)
           ccATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTaa                                                                                                                                     >  1:2753766/1‑148 (MQ=255)
           ccATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTaa                                                                                                                                     <  1:1322088/148‑1 (MQ=255)
            cATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTaaa                                                                                                                                    <  1:1567931/148‑1 (MQ=255)
              ttGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATc                                                                                                                                  >  2:1888522/1‑148 (MQ=255)
               tGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCg                                                                                                                                 >  1:2201764/1‑148 (MQ=255)
                 cGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCa                                                                                                                               >  1:19239/1‑148 (MQ=255)
                   ggATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAgt                                                                                                                             >  2:3423085/1‑148 (MQ=255)
                      tGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAgtttg                                                                                                                          <  2:2837934/148‑1 (MQ=255)
                       gcgcATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAgtttgt                                                                                                                         <  1:2622914/148‑1 (MQ=255)
                       gcgcATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAgtttgt                                                                                                                         <  1:3333066/148‑1 (MQ=255)
                          cATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGtttgtttg                                                                                                                      <  2:1082790/148‑1 (MQ=255)
                          cATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGtttgtttg                                                                                                                      <  1:2573498/148‑1 (MQ=255)
                            tCAGCGGGACAGACAATTGCGCCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGcc                                                                                                                    <  1:2972981/148‑1 (MQ=255)
                             cAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCa                                                                                                                   <  1:2258837/148‑1 (MQ=255)
                              aGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCAt                                                                                                                  <  2:710921/148‑1 (MQ=255)
                              aGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCAt                                                                                                                  <  2:1815001/148‑1 (MQ=255)
                              aGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCAt                                                                                                                  <  2:3123609/148‑1 (MQ=255)
                               gCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATc                                                                                                                 >  2:2414846/1‑148 (MQ=255)
                               gCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTAGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATc                                                                                                                 >  2:3273949/1‑148 (MQ=255)
                               gCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTAGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATc                                                                                                                 >  2:1641614/1‑148 (MQ=255)
                                  ggACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGt                                                                                                              >  2:1579451/1‑148 (MQ=255)
                                  ggACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGt                                                                                                              >  2:2007230/1‑148 (MQ=255)
                                  ggACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGt                                                                                                              <  1:3246803/148‑1 (MQ=255)
                                  ggACAGACAAGTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGt                                                                                                              <  1:1222138/148‑1 (MQ=255)
                                    acagacaATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTAt                                                                                                            >  2:1054951/1‑148 (MQ=255)
                                      agacaATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTg                                                                                                          <  1:1144097/148‑1 (MQ=255)
                                      agacaATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTg                                                                                                          >  2:2493155/1‑148 (MQ=255)
                                      agacaATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTg                                                                                                          <  1:238330/148‑1 (MQ=255)
                                       gacaATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGa                                                                                                         <  2:2598708/148‑1 (MQ=255)
                                          aagtGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGataa                                                                                                      <  2:2861389/145‑1 (MQ=255)
                                          aaTTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGataa                                                                                                      <  1:336715/148‑1 (MQ=255)
                                           atggCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGataat                                                                                                     <  2:2160184/145‑1 (MQ=255)
                                            ttGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGataata                                                                                                    <  2:2714337/148‑1 (MQ=255)
                                             tGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAgtttgt                                                                                                                         >  1:2009295/1‑126 (MQ=255)
                                               cGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATAccc                                                                                                 <  2:3062916/148‑1 (MQ=255)
                                                 tCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTg                                                                                               <  1:2245900/148‑1 (MQ=255)
                                                 tCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTg                                                                                               >  1:877353/1‑148 (MQ=255)
                                                    gTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTACCg                                                                                            >  2:2234634/1‑148 (MQ=255)
                                                     tCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCgg                                                                                           >  1:2611710/1‑148 (MQ=255)
                                                            aaCGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGtt                                                                                    >  2:601753/1‑148 (MQ=255)
                                                            aaCGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGtt                                                                                    <  1:1237145/148‑1 (MQ=255)
                                                               gTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGc                                                                                 <  2:1093418/148‑1 (MQ=255)
                                                                tCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGcc                                                                                >  1:3451287/1‑148 (MQ=255)
                                                                tCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGcc                                                                                >  1:3451006/1‑148 (MQ=255)
                                                                   ggTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTCTTGATAATACCCTGCCGGAAGAGTTCGCCAGa                                                                             >  1:943813/1‑148 (MQ=255)
                                                                   ggTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGa                                                                             >  1:943670/1‑148 (MQ=255)
                                                                   ggTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGa                                                                             >  1:671815/1‑148 (MQ=255)
                                                                        aCCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTa                                                                        >  2:709245/1‑148 (MQ=255)
                                                                        aCCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTa                                                                        >  1:3488242/1‑148 (MQ=255)
                                                                          cATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTAcc                                                                      <  2:1379924/148‑1 (MQ=255)
                                                                            ttttCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCa                                                                    >  2:435204/1‑148 (MQ=255)
                                                                            ttttCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCa                                                                    <  2:1917505/148‑1 (MQ=255)
                                                                             tttCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCAc                                                                   >  1:939081/1‑148 (MQ=255)
                                                                                         ggAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGca                                                       <  2:827805/148‑1 (MQ=255)
                                                                                          gAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGcac                                                      >  1:2003319/1‑148 (MQ=255)
                                                                                            aaTGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGcacca                                                    >  1:2977017/1‑148 (MQ=255)
                                                                                            aaTGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGcacca                                                    >  1:3276545/1‑148 (MQ=255)
                                                                                                 tCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCcgc                                               >  2:1691194/1‑148 (MQ=255)
                                                                                                  cGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCcgcg                                              >  1:1714216/1‑148 (MQ=255)
                                                                                                     ttttATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCgcgg                                           >  2:1892800/1‑148 (MQ=255)
                                                                                                     ttttATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCgcgg                                           >  2:1021545/1‑148 (MQ=255)
                                                                                                          tgatgaACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTg                                      >  1:2001492/1‑148 (MQ=255)
                                                                                                           gatgaACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTgg                                     <  1:502720/148‑1 (MQ=255)
                                                                                                           gatgaACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTgg                                     <  1:206448/148‑1 (MQ=255)
                                                                                                           gatgaACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTgg                                     >  1:650674/1‑148 (MQ=255)
                                                                                                            atgaACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGc                                    >  1:2841238/1‑148 (MQ=255)
                                                                                                               aaCAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCgg                                 >  2:2063518/1‑148 (MQ=255)
                                                                                                               aaCAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCgg                                 <  2:261936/148‑1 (MQ=255)
                                                                                                               aaCAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCgg                                 <  2:262022/148‑1 (MQ=255)
                                                                                                               aaCAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCgg                                 <  1:2160342/148‑1 (MQ=255)
                                                                                                                 cAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGAt                               <  2:2305218/148‑1 (MQ=255)
                                                                                                                   ggggTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGAttt                             <  2:1982471/148‑1 (MQ=255)
                                                                                                                   ggggTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGAttt                             >  1:1551784/1‑148 (MQ=255)
                                                                                                                     ggTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGa                           <  1:1641614/148‑1 (MQ=255)
                                                                                                                      gTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGaa                          >  1:2289555/1‑148 (MQ=255)
                                                                                                                          tgtgATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTg                      >  1:875852/1‑148 (MQ=255)
                                                                                                                           gtgATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGa                     <  1:1889807/148‑1 (MQ=255)
                                                                                                                            tgATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGaa                    >  1:3040748/1‑148 (MQ=255)
                                                                                                                             gATCAGCTTTATACCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAAt                   <  2:1322249/148‑1 (MQ=255)
                                                                                                                               tCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATAc                 <  1:3108189/148‑1 (MQ=255)
                                                                                                                                 aGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACgg               >  2:1606952/1‑148 (MQ=255)
                                                                                                                                  gCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGc              >  1:2498492/1‑148 (MQ=255)
                                                                                                                                  gCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACACTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGc              >  2:1666720/1‑148 (MQ=255)
                                                                                                                                     ttATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTc           >  2:515644/1‑148 (MQ=255)
                                                                                                                                     ttATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTc           >  2:739857/1‑148 (MQ=255)
                                                                                                                                        taCCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGACAGTGAATACGGCGTCCAt        >  2:2690691/1‑148 (MQ=255)
                                                                                                                                        taCCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCAt        >  2:2763007/1‑148 (MQ=255)
                                                                                                                                        taCCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCAt        <  1:1403420/148‑1 (MQ=255)
                                                                                                                                        taCCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCAt        <  2:939081/148‑1 (MQ=255)
                                                                                                                                         aCCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATc       >  2:3504265/1‑148 (MQ=255)
                                                                                                                                              ccTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCTTTCt  >  1:174854/1‑148 (MQ=255)
                                                                                                                                              ccTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCTTTCt  >  1:1969748/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GGGTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCTTTCT  >  CP009273/2659449‑2659738

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGGGTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCTTTCTGATG  >  CP009273/2659448‑2659742
                                                                                                                                                  |                                                                                                                                                    
CGGGTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCC                                                                                                                                                   <  SRR19973067.986844/150‑1 (MQ=60)
CGGGTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCC                                                                                                                                                   <  SRR19973067.986828/150‑1 (MQ=60)
   GTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAG                                                                                                                                                >  SRR19973067.1181265/1‑150 (MQ=60)
   GTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAG                                                                                                                                                >  SRR19973067.1181288/1‑150 (MQ=60)
   GTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAG                                                                                                                                                <  SRR19973067.24982/150‑1 (MQ=60)
   GTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAG                                                                                                                                                <  SRR19973067.2777182/150‑1 (MQ=60)
     TCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGC                                                                                                                                              <  SRR19973067.1057620/150‑1 (MQ=60)
         GATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTA                                                                                                                                          <  SRR19973067.910768/150‑1 (MQ=60)
         GATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTA                                                                                                                                          >  SRR19973067.787697/1‑150 (MQ=60)
         GATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTA                                                                                                                                          >  SRR19973067.2283719/1‑150 (MQ=60)
          ATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAA                                                                                                                                         >  SRR19973067.2771557/1‑150 (MQ=60)
          ATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAA                                                                                                                                         >  SRR19973067.47953/1‑150 (MQ=60)
            CCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAAT                                                                                                                                       <  SRR19973067.1331112/150‑1 (MQ=60)
            CCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAAT                                                                                                                                       >  SRR19973067.1389330/1‑150 (MQ=60)
            CCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAAT                                                                                                                                       <  SRR19973067.3388998/150‑1 (MQ=60)
             CATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATC                                                                                                                                      <  SRR19973067.1578589/150‑1 (MQ=60)
             CATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATC                                                                                                                                      <  SRR19973067.1578653/150‑1 (MQ=60)
             CATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATC                                                                                                                                      >  SRR19973067.2395486/1‑150 (MQ=60)
              ATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCG                                                                                                                                     >  SRR19973067.2216451/1‑150 (MQ=60)
                TGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCA                                                                                                                                   >  SRR19973067.19537/1‑150 (MQ=60)
                        GCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTT                                                                                                                           <  SRR19973067.2639927/150‑1 (MQ=60)
                        GCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTT                                                                                                                           <  SRR19973067.3354159/150‑1 (MQ=60)
                           CATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCC                                                                                                                        <  SRR19973067.2590283/150‑1 (MQ=60)
                           CATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCC                                                                                                                        <  SRR19973067.2590105/150‑1 (MQ=60)
                            ATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGACA                                                                                                                       >  SRR19973067.1987236/1‑150 (MQ=60)
                             TCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCAT                                                                                                                      <  SRR19973067.2992144/150‑1 (MQ=60)
                             TCAGCGGGACAGACAATTGCGCCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCAT                                                                                                                      <  SRR19973067.2991728/150‑1 (MQ=60)
                              CAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATC                                                                                                                     <  SRR19973067.2273826/150‑1 (MQ=60)
                              CAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATC                                                                                                                     <  SRR19973067.2273811/150‑1 (MQ=60)
                                   GGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTAT                                                                                                                <  SRR19973067.1230316/150‑1 (MQ=60)
                                   GGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTAT                                                                                                                <  SRR19973067.3267134/150‑1 (MQ=60)
                                   GGACAGACAAGTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTAT                                                                                                                <  SRR19973067.1230278/150‑1 (MQ=60)
                                    GACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATT                                                                                                               <  SRR19973067.1200882/150‑1 (MQ=60)
                                    GACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATT                                                                                                               <  SRR19973067.1134274/150‑1 (MQ=60)
                                       AGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGAT                                                                                                            <  SRR19973067.1151770/150‑1 (MQ=60)
                                       AGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGAT                                                                                                            <  SRR19973067.240003/150‑1 (MQ=60)
                                       AGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGAT                                                                                                            >  SRR19973067.2756780/1‑150 (MQ=60)
                                       AGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGAT                                                                                                            >  SRR19973067.2040366/1‑150 (MQ=60)
                                         ACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAA                                                                                                          <  SRR19973067.1483194/150‑1 (MQ=60)
                                           AATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATA                                                                                                        <  SRR19973067.339022/150‑1 (MQ=60)
                                            ATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTagatcggaagagcacacgtc                                                                                                       >  SRR19973067.2022825/1‑130 (MQ=60)
                                            ATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATAC                                                                                                       >  SRR19973067.3377195/1‑150 (MQ=60)
                                               GCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCT                                                                                                    <  SRR19973067.2999887/150‑1 (MQ=60)
                                               GCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCT                                                                                                    <  SRR19973067.880782/150‑1 (MQ=60)
                                                CGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTG                                                                                                   >  SRR19973067.883166/1‑150 (MQ=60)
                                                CGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTG                                                                                                   >  SRR19973067.883207/1‑150 (MQ=60)
                                                  TCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCC                                                                                                 <  SRR19973067.2260817/150‑1 (MQ=60)
                                                    AGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGG                                                                                               >  SRR19973067.2628666/1‑150 (MQ=60)
                                                          TCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGT                                                                                         >  SRR19973067.1708198/1‑150 (MQ=60)
                                                            AAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTC                                                                                       >  SRR19973067.1822974/1‑150 (MQ=60)
                                                             AACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCG                                                                                      <  SRR19973067.1245449/150‑1 (MQ=60)
                                                             AACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCG                                                                                      <  SRR19973067.1245671/150‑1 (MQ=60)
                                                               CGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCC                                                                                    >  SRR19973067.3472958/1‑150 (MQ=60)
                                                               CGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCC                                                                                    >  SRR19973067.3473241/1‑150 (MQ=60)
                                                               CGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCC                                                                                    >  SRR19973067.3472974/1‑150 (MQ=60)
                                                                 TCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAG                                                                                  <  SRR19973067.2594849/150‑1 (MQ=60)
                                                                  CTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTCTTGATAATACCCTGCCGGAAGAGTTCGCCAGA                                                                                 >  SRR19973067.950253/1‑150 (MQ=60)
                                                                  CTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGA                                                                                 >  SRR19973067.676412/1‑150 (MQ=60)
                                                                  CTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGA                                                                                 >  SRR19973067.950188/1‑150 (MQ=60)
                                                                  CTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGA                                                                                 >  SRR19973067.950108/1‑150 (MQ=60)
                                                                   TGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGAC                                                                                >  SRR19973067.2018426/1‑150 (MQ=60)
                                                                       AAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTA                                                                            >  SRR19973067.3510379/1‑150 (MQ=60)
                                                                            ATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCAC                                                                       >  SRR19973067.945467/1‑150 (MQ=60)
                                                                            ATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCAC                                                                       >  SRR19973067.3310331/1‑150 (MQ=60)
                                                                               TTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCC                                                                    >  SRR19973067.3258353/1‑150 (MQ=60)
                                                                               TTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCC                                                                    >  SRR19973067.3492032/1‑150 (MQ=60)
                                                                                TCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCT                                                                   <  SRR19973067.531328/150‑1 (MQ=60)
                                                                                   TGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGT                                                                >  SRR19973067.1382626/1‑150 (MQ=60)
                                                                                   TGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGT                                                                >  SRR19973067.661185/1‑150 (MQ=60)
                                                                                    GAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTG                                                               >  SRR19973067.2635444/1‑150 (MQ=60)
                                                                                        TGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCA                                                           >  SRR19973067.1928385/1‑150 (MQ=60)
                                                                                         GGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCAC                                                          >  SRR19973067.2016818/1‑150 (MQ=60)
                                                                                         GGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCAC                                                          >  SRR19973067.2016819/1‑150 (MQ=60)
                                                                                           GAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATTCCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCA                                                        >  SRR19973067.3297027/1‑150 (MQ=60)
                                                                                           GAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCA                                                        >  SRR19973067.2995802/1‑150 (MQ=60)
                                                                                           GAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCA                                                        >  SRR19973067.3297059/1‑150 (MQ=60)
                                                                                                 TTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCG                                                  >  SRR19973067.1726053/1‑150 (MQ=60)
                                                                                                 TTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCG                                                  >  SRR19973067.1725969/1‑150 (MQ=60)
                                                                                                         TATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTG                                          >  SRR19973067.2014990/1‑150 (MQ=60)
                                                                                                          ATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGG                                         >  SRR19973067.655089/1‑150 (MQ=60)
                                                                                                          ATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGG                                         <  SRR19973067.395701/150‑1 (MQ=60)
                                                                                                           TGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGC                                        >  SRR19973067.2859499/1‑150 (MQ=60)
                                                                                                           TGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGC                                        >  SRR19973067.2859482/1‑150 (MQ=60)
                                                                                                           TGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGC                                        >  SRR19973067.2857648/1‑150 (MQ=60)
                                                                                                            GATGAACAGGTGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGTAGATTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCC                                       <  SRR19973067.204698/150‑1 (MQ=60)
                                                                                                            GATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCC                                       <  SRR19973067.506166/150‑1 (MQ=60)
                                                                                                            GATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCC                                       <  SRR19973067.208004/150‑1 (MQ=60)
                                                                                                                AACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGAT                                   <  SRR19973067.2174781/150‑1 (MQ=60)
                                                                                                                AACAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGAT                                   <  SRR19973067.2174790/150‑1 (MQ=60)
                                                                                                                 ACAGGGGTTATGTGATCAGCTTTATACCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATT                                  <  SRR19973067.692221/150‑1 (MQ=60)
                                                                                                                  CAGGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTT                                 >  SRR19973067.1562350/1‑150 (MQ=60)
                                                                                                                    GGGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGA                               <  SRR19973067.1901416/150‑1 (MQ=60)
                                                                                                                     GGGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAA                              >  SRR19973067.2304683/1‑150 (MQ=60)
                                                                                                                      GGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAA                             <  SRR19973067.1652918/150‑1 (MQ=60)
                                                                                                                      GGTTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAA                             <  SRR19973067.3294428/150‑1 (MQ=60)
                                                                                                                        TTATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGT                           >  SRR19973067.3083402/1‑150 (MQ=60)
                                                                                                                         TATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTG                          >  SRR19973067.881698/1‑150 (MQ=60)
                                                                                                                          ATGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGA                         >  SRR19973067.2398011/1‑150 (MQ=60)
                                                                                                                           TGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAA                        >  SRR19973067.3060116/1‑150 (MQ=60)
                                                                                                                           TGTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAA                        >  SRR19973067.163650/1‑150 (MQ=60)
                                                                                                                            GTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAAT                       <  SRR19973067.1902703/150‑1 (MQ=60)
                                                                                                                            GTGATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAAT                       <  SRR19973067.946934/150‑1 (MQ=60)
                                                                                                                             TGATCAGCTTTATACCCACCTAGCCCAGGCGTTAACTCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATA                      <  SRR19973067.90790/150‑1 (MQ=60)
                                                                                                                              GATCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATAC                     >  SRR19973067.2099869/1‑150 (MQ=60)
                                                                                                                                TCAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGG                   <  SRR19973067.3127958/150‑1 (MQ=60)
                                                                                                                                 CAGCTTTATACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGC                  >  SRR19973067.2514895/1‑150 (MQ=60)
                                                                                                                                         TACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCT          <  SRR19973067.1412942/150‑1 (MQ=60)
                                                                                                                                          ACCCACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCTT         <  SRR19973067.223072/150‑1 (MQ=60)
                                                                                                                                             CACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCTTTCT      >  SRR19973067.1983106/1‑150 (MQ=60)
                                                                                                                                             CACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCTTTCT      >  SRR19973067.1983103/1‑150 (MQ=60)
                                                                                                                                             CACCTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCTTTCT      >  SRR19973067.176258/1‑150 (MQ=60)
                                                                                                                                                CTAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCTTTCTGAT   >  SRR19973067.2892672/1‑150 (MQ=60)
                                                                                                                                                 TAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCTTTCTGATG  >  SRR19973067.2956788/1‑150 (MQ=60)
                                                                                                                                                 TAGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCTTTCTGATG  >  SRR19973067.1175199/1‑150 (MQ=60)
                                                                                                                                                  |                                                                                                                                                    
CGGGTTCCCGATCCATTGCGGGATGCGCATCAGCGGGACAGACAATTGCGTCAGTCTATCAAACGTCTGGTAAACCATTTTCGTGAGCTGGGAAATGTTCGTTTTTATGATGAACAGGGGTTATGTGATCAGCTTTATACCCACCTCGCCCAGGCGTTAAATCGCAGTTTGTTTGCCATCGGTATTGATAATACCCTGCCGGAAGAGTTCGCCAGACTGTACCCACGCCTGGTGCGCACCACCCGCGCGGCGCTGGCCGGATTTGAAAGTGAATACGGCGTCCATCTTTCTGATG  >  CP009273/2659448‑2659742

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑