Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,999,827 C→A 89.9% D69E (GAC→GAA)  ygeW → putative carbamoyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,999,8270CA89.9% 511.9 / 53.5 199D69E (GAC→GAA) ygeWputative carbamoyltransferase
Reads supporting (aligned to +/- strand):  ref base C (6/14);  new base A (86/93);  total (92/107)
Fisher's exact test for biased strand distribution p-value = 1.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01

Only 100 of 203 total aligned reads displayed.

AGAAAGATTTTTTGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGACAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTGCGCCGA  >  CP009273/2999682‑2999970
                                                                                                                                                 |                                                                                                                                               
agaAAGATTTTTTGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGaaaa                                                                                                                                               >  2:2723325/1‑148 (MQ=255)
  aaaGATTTTTTGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACt                                                                                                                                             >  1:1002323/1‑148 (MQ=255)
  aaaGATTTTTTGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACt                                                                                                                                             >  1:1443407/1‑148 (MQ=255)
   aaGATTTTTTGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTc                                                                                                                                            <  1:1942290/148‑1 (MQ=255)
    aGATTTTTTGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTcc                                                                                                                                           >  1:3276422/1‑148 (MQ=255)
      aTTTTTTGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCAc                                                                                                                                         <  1:916720/148‑1 (MQ=255)
      aTTTTTTGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCAc                                                                                                                                         <  2:1361483/148‑1 (MQ=255)
      aTTTTTTGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCAc                                                                                                                                         >  2:1927613/1‑148 (MQ=255)
      aTTTTTTGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCAc                                                                                                                                         >  1:2175157/1‑148 (MQ=255)
      aTTTTTTGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTACTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCAc                                                                                                                                         <  2:1361641/148‑1 (MQ=255)
       ttttttGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCAcc                                                                                                                                        <  1:1271507/148‑1 (MQ=255)
       ttttttGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCAcc                                                                                                                                        <  2:2036148/148‑1 (MQ=255)
        tttttGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAACACTCCAccc                                                                                                                                       >  1:2633729/1‑148 (MQ=255)
        tttttGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCAccc                                                                                                                                       >  2:2848074/1‑148 (MQ=255)
         ttttGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCg                                                                                                                                      >  2:2159005/1‑148 (MQ=255)
               tAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGACAACTCCACCCGTACCCg                                                                                                                                >  2:1250070/1‑148 (MQ=255)
               tAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCg                                                                                                                                >  1:690932/1‑148 (MQ=255)
                 aCGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCt                                                                                                                              <  1:3022977/148‑1 (MQ=255)
                    tGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTtct                                                                                                                           <  2:3021843/148‑1 (MQ=255)
                    tGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTtct                                                                                                                           >  1:2827162/1‑148 (MQ=255)
                       gAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCtt                                                                                                                        >  2:1816365/1‑148 (MQ=255)
                         aCAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTAt                                                                                                                      <  1:1855130/148‑1 (MQ=255)
                         aCAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTAt                                                                                                                      >  1:750203/1‑148 (MQ=255)
                          cAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATg                                                                                                                     >  2:487299/1‑148 (MQ=255)
                          cAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATg                                                                                                                     <  1:607365/148‑1 (MQ=255)
                            gACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCt                                                                                                                   >  1:367381/1‑148 (MQ=255)
                                   gATGAACTGACACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAACGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCgcgc                                                                                                            <  2:54943/148‑1 (MQ=255)
                                   gATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCgcgc                                                                                                            >  1:732832/1‑148 (MQ=255)
                                   gATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCgcgc                                                                                                            >  1:2392064/1‑148 (MQ=255)
                                    aTGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCt                                                                                                           >  1:2261323/1‑148 (MQ=255)
                                      gAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTa                                                                                                         <  1:1391506/148‑1 (MQ=255)
                                      gAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTa                                                                                                         >  1:1161468/1‑148 (MQ=255)
                                       aaCTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTaa                                                                                                        <  2:1509203/148‑1 (MQ=255)
                                        aCTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGACAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAAc                                                                                                       <  1:2203596/148‑1 (MQ=255)
                                        aCTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGACAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAAc                                                                                                       <  1:1780114/148‑1 (MQ=255)
                                         cTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAAcc                                                                                                      <  1:1573824/148‑1 (MQ=255)
                                           gAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTg                                                                                                    <  1:3128991/148‑1 (MQ=255)
                                            aaaCAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGACAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGc                                                                                                   <  2:1479452/148‑1 (MQ=255)
                                                 aGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGACAACTCCACCCGGACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGc                                                                                              <  2:907832/148‑1 (MQ=255)
                                                  gTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGACAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGcc                                                                                             <  2:1405696/148‑1 (MQ=255)
                                                  gTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGcc                                                                                             >  1:1647316/1‑148 (MQ=255)
                                                   tACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCt                                                                                            >  1:92764/1‑148 (MQ=255)
                                                         aCGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGcaca                                                                                      >  2:288132/1‑148 (MQ=255)
                                                         aCGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGcaca                                                                                      >  2:288047/1‑148 (MQ=255)
                                                          cGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCacaa                                                                                     >  1:3223802/1‑148 (MQ=255)
                                                          cGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCacaa                                                                                     >  1:2559149/1‑148 (MQ=255)
                                                            ttGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCacaaca                                                                                   >  2:792727/1‑148 (MQ=255)
                                                              gCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAg                                                                                 <  2:3202793/148‑1 (MQ=255)
                                                              gCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAg                                                                                 <  1:521367/148‑1 (MQ=255)
                                                              gCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAg                                                                                 <  1:2941645/148‑1 (MQ=255)
                                                                cGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGAt                                                                               <  1:2313194/148‑1 (MQ=255)
                                                                     caTTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTcga                                                                          <  2:2374667/148‑1 (MQ=255)
                                                                     caTTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTcga                                                                          <  2:712347/148‑1 (MQ=255)
                                                                        tAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTcgacga                                                                       >  2:255596/1‑148 (MQ=255)
                                                                         aaaaGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGaa                                                                      <  2:2869890/148‑1 (MQ=255)
                                                                          aaaGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAg                                                                     <  1:3070538/148‑1 (MQ=255)
                                                                          aaaGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAg                                                                     <  2:1002323/148‑1 (MQ=255)
                                                                              cACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCaa                                                                 >  1:2362042/1‑148 (MQ=255)
                                                                                cTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAAt                                                               >  1:102832/1‑148 (MQ=255)
                                                                                cTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAAt                                                               >  1:301102/1‑148 (MQ=255)
                                                                                 tGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATc                                                              <  1:158155/148‑1 (MQ=255)
                                                                                  gCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATca                                                             <  1:585801/148‑1 (MQ=255)
                                                                                      gCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCACATCACaaa                                                         >  2:399438/1‑148 (MQ=255)
                                                                                      gCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACaaa                                                         >  2:399278/1‑148 (MQ=255)
                                                                                                 ctcAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATAGCTCACGGc                                              >  1:381007/1‑148 (MQ=255)
                                                                                                 ctcAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATAGCTCACGGc                                              >  1:2073045/1‑148 (MQ=255)
                                                                                                      ccAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATAGCTCACGGCGAAAc                                         >  2:2943397/1‑148 (MQ=255)
                                                                                                        aaaGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGACAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACcg                                       >  1:2487383/1‑148 (MQ=255)
                                                                                                                 aaTAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGaaa                              <  1:1212921/148‑1 (MQ=255)
                                                                                                                     gTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGc                          <  1:949757/148‑1 (MQ=255)
                                                                                                                     gTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGc                          <  2:661964/148‑1 (MQ=255)
                                                                                                                      tGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGcc                         >  1:1844792/1‑148 (MQ=255)
                                                                                                                      tGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATAGCTCACGGCGAAACCGTGCGTGAAACCGcc                         >  1:1338412/1‑148 (MQ=255)
                                                                                                                      tGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATAGCTCACGGCGAAACCGTGCGTGAAACCGcc                         >  1:985719/1‑148 (MQ=255)
                                                                                                                       ggATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAACTCGCTCACGGCGAAACCGTGCGTGAAACCGCCa                        <  2:469847/148‑1 (MQ=255)
                                                                                                                       ggATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCa                        <  2:450422/148‑1 (MQ=255)
                                                                                                                       ggATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCa                        <  2:1680665/148‑1 (MQ=255)
                                                                                                                       ggATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCa                        <  2:2673326/148‑1 (MQ=255)
                                                                                                                           tAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAatat                    >  1:2027349/1‑148 (MQ=255)
                                                                                                                           tAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAatat                    >  1:2027290/1‑148 (MQ=255)
                                                                                                                            aGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATg                   <  2:259307/148‑1 (MQ=255)
                                                                                                                             ggTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGa                  >  2:2217965/1‑148 (MQ=255)
                                                                                                                             ggTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATAGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGa                  <  1:2150690/148‑1 (MQ=255)
                                                                                                                               tATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATAGCTCACGGCTAAACCGTGAGTGAAACAGCCAATATGAtc                >  2:1409651/1‑148 (MQ=255)
                                                                                                                                aTTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGAtct               >  1:2754953/1‑148 (MQ=255)
                                                                                                                                 tttCCGTATTCCTCGAATACTCCACCCGTACCCTCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGTCGAATCCGTGCGTGAAACCGCCAATATGAtctc              <  2:2231162/148‑1 (MQ=255)
                                                                                                                                 tttCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGAtctc              <  2:1258112/148‑1 (MQ=255)
                                                                                                                                  ttCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTcc             <  2:3065908/148‑1 (MQ=255)
                                                                                                                                    ccGTATTCCGCGACAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCtt           <  1:3153504/148‑1 (MQ=255)
                                                                                                                                    ccGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATAGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCtt           <  2:530675/148‑1 (MQ=255)
                                                                                                                                    ccGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATAGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCtt           <  1:3163653/148‑1 (MQ=255)
                                                                                                                                    ccGTATTCCGCGAAAACTCCACCCGTACCCGCGTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCtt           <  2:1634112/148‑1 (MQ=255)
                                                                                                                                     cGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATAGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTc          <  1:1872736/148‑1 (MQ=255)
                                                                                                                                     cGTAGTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTc          <  2:1920887/148‑1 (MQ=255)
                                                                                                                                       tATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTg        >  2:412936/1‑148 (MQ=255)
                                                                                                                                       tATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTg        >  2:1613490/1‑148 (MQ=255)
                                                                                                                                       tATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTg        >  1:3077370/1‑148 (MQ=255)
                                                                                                                                         ttCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTgcg      >  2:3298278/1‑148 (MQ=255)
                                                                                                                                             gcgAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTGCGCCGa  <  2:3223802/148‑1 (MQ=255)
                                                                                                                                             gcgAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATAGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTGCGCCGa  <  1:1938232/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
AGAAAGATTTTTTGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGACAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTGCGCCGA  >  CP009273/2999682‑2999970

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGAAAGATTTTTTGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGACAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTGCGCCGACGCT  >  CP009273/2999682‑2999974
                                                                                                                                                 |                                                                                                                                                   
AGAAAGATTTTTTGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACT                                                                                                                                                 >  SRR19973070.1453422/1‑150 (MQ=60)
AGAAAGATTTTTTGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACT                                                                                                                                                 >  SRR19973070.1009464/1‑150 (MQ=60)
  AAAGATTTTTTGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCC                                                                                                                                               >  SRR19973070.3297477/1‑150 (MQ=60)
   AAGATTTTTTGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCA                                                                                                                                              <  SRR19973070.1955338/150‑1 (MQ=60)
    AGATTTTTTGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCAC                                                                                                                                             >  SRR19973070.2189154/1‑150 (MQ=60)
    AGATTTTTTGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCAC                                                                                                                                             >  SRR19973070.2189568/1‑150 (MQ=60)
      ATTTTTTGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAACACTCCACCC                                                                                                                                           >  SRR19973070.2650902/1‑150 (MQ=60)
      ATTTTTTGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCC                                                                                                                                           <  SRR19973070.923244/150‑1 (MQ=60)
       TTTTTTGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCG                                                                                                                                          <  SRR19973070.1280406/150‑1 (MQ=60)
             GTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCG                                                                                                                                    >  SRR19973070.695869/1‑150 (MQ=60)
                 ACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTC                                                                                                                                <  SRR19973070.3042238/150‑1 (MQ=60)
                 ACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTC                                                                                                                                <  SRR19973070.3041569/150‑1 (MQ=60)
                  CGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCT                                                                                                                               >  SRR19973070.2845455/1‑150 (MQ=60)
                    TGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCT                                                                                                                             <  SRR19973070.937722/150‑1 (MQ=60)
                    TGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCT                                                                                                                             <  SRR19973070.986247/150‑1 (MQ=60)
                    TGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCT                                                                                                                             >  SRR19973070.616292/1‑150 (MQ=60)
                     GGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTT                                                                                                                            <  SRR19973070.2740900/150‑1 (MQ=60)
                       GAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTAT                                                                                                                          >  SRR19973070.755500/1‑150 (MQ=60)
                       GAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTAT                                                                                                                          <  SRR19973070.2520897/150‑1 (MQ=60)
                       GAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGAACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGTATTAGGTATTTCCGTATTCAGCGAAAACTCCACCCGTACCCGCTTCTCTTAT                                                                                                                          >  SRR19973070.755467/1‑150 (MQ=60)
                        AACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATG                                                                                                                         <  SRR19973070.1465782/150‑1 (MQ=60)
                         ACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGC                                                                                                                        <  SRR19973070.1868783/150‑1 (MQ=60)
                         ACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGC                                                                                                                        <  SRR19973070.1867835/150‑1 (MQ=60)
                          CAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCT                                                                                                                       >  SRR19973070.370079/1‑150 (MQ=60)
                          CAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCT                                                                                                                       >  SRR19973070.370223/1‑150 (MQ=60)
                          CAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCT                                                                                                                       <  SRR19973070.611615/150‑1 (MQ=60)
                          CAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCT                                                                                                                       <  SRR19973070.2134320/150‑1 (MQ=60)
                           AGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTT                                                                                                                      >  SRR19973070.2817891/1‑150 (MQ=60)
                                 CAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGC                                                                                                                >  SRR19973070.738021/1‑150 (MQ=60)
                                 CAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGC                                                                                                                >  SRR19973070.2407854/1‑150 (MQ=60)
                                  AGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCT                                                                                                               >  SRR19973070.2276327/1‑150 (MQ=60)
                                  AGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCT                                                                                                               >  SRR19973070.603175/1‑150 (MQ=60)
                                  AGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCT                                                                                                               >  SRR19973070.1173817/1‑150 (MQ=60)
                                    ATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTA                                                                                                             >  SRR19973070.3089707/1‑150 (MQ=60)
                                    ATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTA                                                                                                             >  SRR19973070.1169592/1‑150 (MQ=60)
                                      GAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAAC                                                                                                           <  SRR19973070.1400898/150‑1 (MQ=60)
                                      GAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAAC                                                                                                           <  SRR19973070.1401288/150‑1 (MQ=60)
                                        ACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGACAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCT                                                                                                         <  SRR19973070.2218112/150‑1 (MQ=60)
                                        ACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGACAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCT                                                                                                         <  SRR19973070.107548/150‑1 (MQ=60)
                                        ACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGACAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCT                                                                                                         <  SRR19973070.1792436/150‑1 (MQ=60)
                                         CTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTG                                                                                                        <  SRR19973070.1584955/150‑1 (MQ=60)
                                         CTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTG                                                                                                        <  SRR19973070.1584873/150‑1 (MQ=60)
                                           GAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCT                                                                                                      <  SRR19973070.3149302/150‑1 (MQ=60)
                                           GAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCT                                                                                                      <  SRR19973070.3149198/150‑1 (MQ=60)
                                           GAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCT                                                                                                      <  SRR19973070.1349229/150‑1 (MQ=60)
                                              ACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGG                                                                                                   <  SRR19973070.925920/150‑1 (MQ=60)
                                                AAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCC                                                                                                 >  SRR19973070.1658690/1‑150 (MQ=60)
                                                 AGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCT                                                                                                >  SRR19973070.93680/1‑150 (MQ=60)
                                                        GACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAA                                                                                         >  SRR19973070.3244635/1‑150 (MQ=60)
                                                        GACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAA                                                                                         >  SRR19973070.2575868/1‑150 (MQ=60)
                                                              GCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGACAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGAT                                                                                   <  SRR19973070.1258838/150‑1 (MQ=60)
                                                              GCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGAT                                                                                   <  SRR19973070.524911/150‑1 (MQ=60)
                                                              GCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGAT                                                                                   <  SRR19973070.2960602/150‑1 (MQ=60)
                                                                CGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCT                                                                                 <  SRR19973070.2328569/150‑1 (MQ=60)
                                                                CGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCT                                                                                 <  SRR19973070.2328448/150‑1 (MQ=60)
                                                                CGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCT                                                                                 <  SRR19973070.2630699/150‑1 (MQ=60)
                                                                 GCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTC                                                                                <  SRR19973070.398297/150‑1 (MQ=60)
                                                                  CAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCG                                                                               <  SRR19973070.995960/150‑1 (MQ=60)
                                                                      ATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGA                                                                           >  SRR19973070.2878911/1‑150 (MQ=60)
                                                                         AAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGG                                                                        <  SRR19973070.1668702/150‑1 (MQ=60)
                                                                          AAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGC                                                                       <  SRR19973070.3090241/150‑1 (MQ=60)
                                                                          AAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGC                                                                       <  SRR19973070.3090252/150‑1 (MQ=60)
                                                                          AAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGC                                                                       >  SRR19973070.1824076/1‑150 (MQ=60)
                                                                            AGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAA                                                                     >  SRR19973070.2377764/1‑150 (MQ=60)
                                                                            AGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAA                                                                     >  SRR19973070.2377674/1‑150 (MQ=60)
                                                                            AGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGATTAACCTGCTCGGCCTTGCACAACAATATCTCGACGAAGGCAA                                                                     >  SRR19973070.2489914/1‑150 (MQ=60)
                                                                              CACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGACAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAAT                                                                   >  SRR19973070.1657551/1‑150 (MQ=60)
                                                                              CACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAAT                                                                   >  SRR19973070.103752/1‑150 (MQ=60)
                                                                              CACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAAT                                                                   <  SRR19973070.2344187/150‑1 (MQ=60)
                                                                              CACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAAT                                                                   >  SRR19973070.103807/1‑150 (MQ=60)
                                                                              CACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAAT                                                                   >  SRR19973070.303330/1‑150 (MQ=60)
                                                                              CACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAAT                                                                   >  SRR19973070.303209/1‑150 (MQ=60)
                                                                                 TGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCAC                                                                <  SRR19973070.159469/150‑1 (MQ=60)
                                                                                 TGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCAC                                                                >  SRR19973070.3245105/1‑150 (MQ=60)
                                                                                  GCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACA                                                               <  SRR19973070.589840/150‑1 (MQ=60)
                                                                                  GCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACA                                                               <  SRR19973070.589817/150‑1 (MQ=60)
                                                                                      GCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGACAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATC                                                           <  SRR19973070.1282769/150‑1 (MQ=60)
                                                                                            AACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCAC                                                     >  SRR19973070.3126548/1‑150 (MQ=60)
                                                                                            AACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCAC                                                     <  SRR19973070.3327965/150‑1 (MQ=60)
                                                                                               ATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATAGCTCACGGC                                                  >  SRR19973070.2087002/1‑150 (MQ=60)
                                                                                               ATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATAGCTCACGGC                                                  >  SRR19973070.383802/1‑150 (MQ=60)
                                                                                                   CAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGACAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAA                                              >  SRR19973070.1922987/1‑150 (MQ=60)
                                                                                                    AACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGAGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGATCACGTCGAAAC                                             >  SRR19973070.1963678/1‑150 (MQ=60)
                                                                                                      CCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGACAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCG                                           >  SRR19973070.2503542/1‑150 (MQ=60)
                                                                                                         AAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATAGCTCACGGCGAAACCGTGC                                        >  SRR19973070.722862/1‑150 (MQ=60)
                                                                                                            TCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTG                                     <  SRR19973070.2190399/150‑1 (MQ=60)
                                                                                                                 AATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACC                                <  SRR19973070.1221435/150‑1 (MQ=60)
                                                                                                                 AATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACC                                <  SRR19973070.2256080/150‑1 (MQ=60)
                                                                                                                    AGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCC                             >  SRR19973070.1857443/1‑150 (MQ=60)
                                                                                                                    AGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCC                             >  SRR19973070.1857625/1‑150 (MQ=60)
                                                                                                                    AGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATAGCTCACGGCGAAACCGTGCGTGAAACCGCC                             >  SRR19973070.992746/1‑150 (MQ=60)
                                                                                                                    AGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATAGCTCACGGCGAAACCGTGCGTGAAACCGCC                             >  SRR19973070.1347615/1‑150 (MQ=60)
                                                                                                                     GTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCA                            <  SRR19973070.956524/150‑1 (MQ=60)
                                                                                                                         ATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATAT                        >  SRR19973070.2041058/1‑150 (MQ=60)
                                                                                                                         ATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATAT                        >  SRR19973070.2041136/1‑150 (MQ=60)
                                                                                                                         ATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATAT                        >  SRR19973070.2040998/1‑150 (MQ=60)
                                                                                                                         ATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATAT                        >  SRR19973070.2041039/1‑150 (MQ=60)
                                                                                                                             GGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATAGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATC                    <  SRR19973070.2165008/150‑1 (MQ=60)
                                                                                                                              GTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCT                   >  SRR19973070.2772640/1‑150 (MQ=60)
                                                                                                                               TATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTC                  <  SRR19973070.2997599/150‑1 (MQ=60)
                                                                                                                                    CCGTATTCCGCGACAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCT             <  SRR19973070.3173881/150‑1 (MQ=60)
                                                                                                                                    CCGTATTCCGCGACAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCT             <  SRR19973070.3174505/150‑1 (MQ=60)
                                                                                                                                    CCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATAGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCT             <  SRR19973070.3184122/150‑1 (MQ=60)
                                                                                                                                    CCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATAGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCT             <  SRR19973070.3184283/150‑1 (MQ=60)
                                                                                                                                     CGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTG            >  SRR19973070.3097142/1‑150 (MQ=60)
                                                                                                                                     CGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATAGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTG            <  SRR19973070.1885507/150‑1 (MQ=60)
                                                                                                                                      GTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTGC           <  SRR19973070.849380/150‑1 (MQ=60)
                                                                                                                                             GCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTGCGCCGACG    >  SRR19973070.1659698/1‑150 (MQ=60)
                                                                                                                                             GCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATAGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTGCGCCGACG    <  SRR19973070.1951261/150‑1 (MQ=60)
                                                                                                                                             GCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATAGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTGCGCCGACG    <  SRR19973070.1951588/150‑1 (MQ=60)
                                                                                                                                               GAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTGCGCCGACGCT  >  SRR19973070.1719242/1‑150 (MQ=60)
                                                                                                                                                 |                                                                                                                                                   
AGAAAGATTTTTTGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGACAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTGCGCCGACGCT  >  CP009273/2999682‑2999974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑