Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,999,827 C→A 100% D69E (GAC→GAA)  ygeW → putative carbamoyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,999,8270CA98.1% 853.2 / 4.5 262D69E (GAC→GAA) ygeWputative carbamoyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/5);  new base A (144/112);  total (145/117)
Fisher's exact test for biased strand distribution p-value = 1.73e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 262 total aligned reads displayed.

GAGAAAGATTTTTTGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGACAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTGCGCCGAC  >  CP009273/2999681‑2999971
                                                                                                                                                  |                                                                                                                                                
gagaAAGATTTTTTGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAAc                                                                                                                                               >  2:81704/1‑150 (MQ=255)
 agaAAGATTTTTTGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACt                                                                                                                                              >  1:5161785/1‑150 (MQ=255)
        ttttttGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCg                                                                                                                                       >  1:4045692/1‑150 (MQ=255)
          ttttGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTa                                                                                                                                     <  1:2166798/150‑1 (MQ=255)
            ttGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTAcc                                                                                                                                   >  2:1514788/1‑150 (MQ=255)
             tGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTAccc                                                                                                                                  <  2:5113719/150‑1 (MQ=255)
             tGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTAccc                                                                                                                                  <  1:2267070/150‑1 (MQ=255)
              gTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCg                                                                                                                                 >  2:3699989/1‑150 (MQ=255)
                tAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCt                                                                                                                               >  2:2315296/1‑150 (MQ=255)
                   cGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTtct                                                                                                                            >  2:586567/1‑150 (MQ=255)
                   cGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTtct                                                                                                                            <  1:1645632/150‑1 (MQ=255)
                     tGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTctct                                                                                                                          <  1:817896/150‑1 (MQ=255)
                     tGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTctct                                                                                                                          <  2:2019233/150‑1 (MQ=255)
                      gggAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCtt                                                                                                                         <  1:2903606/150‑1 (MQ=255)
                       tgAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTa                                                                                                                        >  2:1108331/2‑150 (MQ=255)
                       ggAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTa                                                                                                                        <  2:4073898/150‑1 (MQ=255)
                          aCAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGc                                                                                                                     >  1:2417525/1‑150 (MQ=255)
                           cAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCt                                                                                                                    >  1:4940692/1‑150 (MQ=255)
                             gACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTc                                                                                                                  <  1:1540084/150‑1 (MQ=255)
                               cGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACACGTACCCGCTTCTCTTATGCTTCcg                                                                                                                >  2:3983713/1‑150 (MQ=255)
                                gCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCcgc                                                                                                               >  2:2888756/1‑150 (MQ=255)
                                   aGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCt                                                                                                            >  2:3118222/1‑150 (MQ=255)
                                      tGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTaa                                                                                                         >  2:3209708/1‑150 (MQ=255)
                                         aCTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCt                                                                                                      <  2:3359332/150‑1 (MQ=255)
                                          cTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTg                                                                                                     <  2:2981697/150‑1 (MQ=255)
                                           tGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGc                                                                                                    >  2:170441/1‑150 (MQ=255)
                                           tGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGc                                                                                                    <  1:1505570/150‑1 (MQ=255)
                                            gAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCt                                                                                                   <  2:3847175/150‑1 (MQ=255)
                                            gAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCt                                                                                                   <  2:1167313/150‑1 (MQ=255)
                                             aaaCAAGTACTGGACGTTGCCGCAGCATTAAAAGCANTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTc                                                                                                  >  1:2681021/1‑150 (MQ=255)
                                             aaaCAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTc                                                                                                  <  2:3178118/150‑1 (MQ=255)
                                             aaaCAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTc                                                                                                  <  1:2434063/150‑1 (MQ=255)
                                              aaCAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCg                                                                                                 <  1:2228055/150‑1 (MQ=255)
                                                cAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGc                                                                                               <  2:3553279/150‑1 (MQ=255)
                                                cAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGc                                                                                               <  1:1877229/150‑1 (MQ=255)
                                                   gTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCtt                                                                                            <  2:2042927/150‑1 (MQ=255)
                                                      cTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGca                                                                                         >  1:4848086/1‑150 (MQ=255)
                                                        ggACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGcaca                                                                                       >  1:2989082/1‑150 (MQ=255)
                                                            gTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATGTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCAcaacaa                                                                                   <  2:3168321/150‑1 (MQ=255)
                                                              tGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGa                                                                                 >  1:3642695/1‑150 (MQ=255)
                                                                ccGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGAtc                                                                               >  2:1643220/1‑150 (MQ=255)
                                                                 cGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGAtct                                                                              >  1:1189800/1‑150 (MQ=255)
                                                                 cGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGAtct                                                                              >  1:4391821/1‑150 (MQ=255)
                                                                 cGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGAtct                                                                              >  2:4819811/1‑150 (MQ=255)
                                                                   cagcaTTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTcg                                                                            <  1:1513777/150‑1 (MQ=255)
                                                                   cagcaTTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTcg                                                                            <  2:2785763/150‑1 (MQ=255)
                                                                   cagcaTTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTcg                                                                            <  1:4228632/150‑1 (MQ=255)
                                                                    agcaTTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTcga                                                                           >  2:4762206/1‑150 (MQ=255)
                                                                    agcaTTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTcga                                                                           >  1:2932910/1‑150 (MQ=255)
                                                                     gcaTTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTcgac                                                                          <  1:3284627/150‑1 (MQ=255)
                                                                       aTTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACACGATCTcgacga                                                                        >  2:4199171/1‑150 (MQ=255)
                                                                          aaaaGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAgg                                                                     >  2:1979566/1‑150 (MQ=255)
                                                                           aaaGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGc                                                                    >  1:5113478/1‑150 (MQ=255)
                                                                            aaGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCa                                                                   >  2:4659469/1‑150 (MQ=255)
                                                                                    cGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGAGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACaa                                                           >  2:4530431/1‑150 (MQ=255)
                                                                                     gTGCTGATAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACaaa                                                          <  2:1281188/150‑1 (MQ=255)
                                                                                       gCTGAAAACATCTCAACCAAAGTCTTTAATATTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAAACACAAATc                                                        >  1:898618/1‑150 (MQ=255)
                                                                                        cTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCg                                                       >  1:1827752/1‑150 (MQ=255)
                                                                                         tGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGc                                                      >  1:2384504/1‑150 (MQ=255)
                                                                                             aaCATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCAc                                                  <  1:122391/150‑1 (MQ=255)
                                                                                              aCATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACg                                                 >  1:2311122/1‑150 (MQ=255)
                                                                                                  ctcAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGa                                             <  2:2287227/150‑1 (MQ=255)
                                                                                                  ctcAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGa                                             >  2:4368785/1‑150 (MQ=255)
                                                                                                    cAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGaaa                                           <  2:2252046/150‑1 (MQ=255)
                                                                                                      aCCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAAcc                                         <  1:38400/150‑1 (MQ=255)
                                                                                                      aCCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAAcc                                         >  1:4620021/1‑150 (MQ=255)
                                                                                                      aCCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAAcc                                         <  2:1641774/150‑1 (MQ=255)
                                                                                                       ccAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACcg                                        >  2:234696/1‑150 (MQ=255)
                                                                                                       ccAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACcg                                        >  2:4482121/1‑150 (MQ=255)
                                                                                                        cAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACcgt                                       >  2:1753930/1‑150 (MQ=255)
                                                                                                         aaaGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACcgtg                                      <  2:5040581/150‑1 (MQ=255)
                                                                                                         aaaGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACcgtg                                      >  1:1380603/1‑150 (MQ=255)
                                                                                                         aaaGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACcgtg                                      >  1:579853/1‑150 (MQ=255)
                                                                                                         aaaGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACcgtg                                      <  1:2843818/150‑1 (MQ=255)
                                                                                                           aGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACcgtgcg                                    >  1:3114752/1‑150 (MQ=255)
                                                                                                             tCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACcgtgcgtg                                  >  2:3891387/1‑150 (MQ=255)
                                                                                                                ttAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGaaa                               >  1:5004733/1‑150 (MQ=255)
                                                                                                                  aaTAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAAcc                             >  2:1740889/1‑150 (MQ=255)
                                                                                                                   aTAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCg                            <  2:3089522/150‑1 (MQ=255)
                                                                                                                        ggATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAat                       >  2:4106419/1‑150 (MQ=255)
                                                                                                                         gATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAata                      <  1:4438705/150‑1 (MQ=255)
                                                                                                                         gATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAata                      <  2:2621359/150‑1 (MQ=255)
                                                                                                                          aTTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAatat                     <  2:4995547/150‑1 (MQ=255)
                                                                                                                             aGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGAt                  <  2:3271785/150‑1 (MQ=255)
                                                                                                                               gTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGAtct                >  1:1140200/1‑150 (MQ=255)
                                                                                                                                tATGTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGAtctc               <  2:3428566/150‑1 (MQ=255)
                                                                                                                                   ttCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCtt            >  2:1635425/1‑150 (MQ=255)
                                                                                                                                    tCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTc           <  1:35361/150‑1 (MQ=255)
                                                                                                                                     ccGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTGGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCt          <  1:3983713/150‑1 (MQ=255)
                                                                                                                                      cGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTg         <  1:4511126/150‑1 (MQ=255)
                                                                                                                                      cGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTg         <  2:4229915/150‑1 (MQ=255)
                                                                                                                                      cGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTg         >  1:4181634/1‑150 (MQ=255)
                                                                                                                                       gTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTgc        >  1:3744658/1‑150 (MQ=255)
                                                                                                                                       gTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTgc        >  1:4680417/1‑150 (MQ=255)
                                                                                                                                        tATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTgcg       >  1:1746363/1‑150 (MQ=255)
                                                                                                                                        tATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTgcg       >  1:3491006/1‑150 (MQ=255)
                                                                                                                                        tATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTgcg       <  1:1108331/150‑1 (MQ=255)
                                                                                                                                          ttCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTGCGcc     >  1:10195/1‑150 (MQ=255)
                                                                                                                                            ccGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTGCGCCGa   <  2:2397600/150‑1 (MQ=255)
                                                                                                                                             cgcgAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTGCGCCGAc  >  2:691682/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
GAGAAAGATTTTTTGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGACAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTGCGCCGAC  >  CP009273/2999681‑2999971

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CACGAGAAAGATTTTTTGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGACAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTGCGCCGACGCTAT  >  CP009273/2999678‑2999976
                                                                                                                                                     |                                                                                                                                                     
CACGAGAAAGATTTTTTGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAA                                                                                                                                                       <  SRR19973063.4790072/150‑1 (MQ=60)
   GAGAAAGATTTTTTGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAAC                                                                                                                                                    >  SRR19973063.4243395/1‑150 (MQ=60)
    AGAAAGATTTTTTGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACT                                                                                                                                                   >  SRR19973063.5178392/1‑150 (MQ=60)
       AAGATTTTTTGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGACAACTCCA                                                                                                                                                <  SRR19973063.1513507/150‑1 (MQ=60)
       AAGATTTTTTGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCA                                                                                                                                                >  SRR19973063.1857610/1‑150 (MQ=60)
          ATTTTTTGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCC                                                                                                                                             >  SRR19973063.2258722/1‑150 (MQ=60)
           TTTTTTGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCG                                                                                                                                            >  SRR19973063.4058654/1‑150 (MQ=60)
            TTTTTGTTAAGGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGT                                                                                                                                           <  SRR19973063.2656752/150‑1 (MQ=60)
            TTTTTGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGT                                                                                                                                           <  SRR19973063.402679/150‑1 (MQ=60)
             TTTTGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTA                                                                                                                                          <  SRR19973063.2173336/150‑1 (MQ=60)
             TTTTGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTA                                                                                                                                          <  SRR19973063.2173581/150‑1 (MQ=60)
                TGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCC                                                                                                                                       <  SRR19973063.2274298/150‑1 (MQ=60)
                 GTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCG                                                                                                                                      <  SRR19973063.3691972/150‑1 (MQ=60)
                   TAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCT                                                                                                                                    <  SRR19973063.52439/150‑1 (MQ=60)
                    AACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTT                                                                                                                                   <  SRR19973063.1648907/150‑1 (MQ=60)
                      CGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCT                                                                                                                                 >  SRR19973063.4965872/1‑150 (MQ=60)
                      CGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCT                                                                                                                                 <  SRR19973063.1650707/150‑1 (MQ=60)
                      CGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCT                                                                                                                                 <  SRR19973063.1650660/150‑1 (MQ=60)
                        TGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGACAACTCCACCCGTACCCGCTTCTCT                                                                                                                               <  SRR19973063.1117162/150‑1 (MQ=60)
                        TGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCT                                                                                                                               <  SRR19973063.820260/150‑1 (MQ=60)
                         GGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTT                                                                                                                              <  SRR19973063.2912872/150‑1 (MQ=60)
                         GGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTT                                                                                                                              <  SRR19973063.2864814/150‑1 (MQ=60)
                         GGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTT                                                                                                                              <  SRR19973063.262440/150‑1 (MQ=60)
                           GAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTAT                                                                                                                            >  SRR19973063.685245/1‑150 (MQ=60)
                            AACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATG                                                                                                                           >  SRR19973063.410168/1‑150 (MQ=60)
                             ACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGC                                                                                                                          >  SRR19973063.2425255/1‑150 (MQ=60)
                              CAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCT                                                                                                                         >  SRR19973063.4956666/1‑150 (MQ=60)
                               AGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTT                                                                                                                        >  SRR19973063.4039615/1‑150 (MQ=60)
                                GACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTC                                                                                                                       <  SRR19973063.1544803/150‑1 (MQ=60)
                                      AGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCT                                                                                                                 >  SRR19973063.451988/1‑150 (MQ=60)
                                       GATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTT                                                                                                                >  SRR19973063.5075980/1‑150 (MQ=60)
                                       GATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTT                                                                                                                >  SRR19973063.2433076/1‑150 (MQ=60)
                                       GATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTT                                                                                                                <  SRR19973063.46094/150‑1 (MQ=60)
                                          GAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAAC                                                                                                             <  SRR19973063.3628295/150‑1 (MQ=60)
                                             CTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTG                                                                                                          >  SRR19973063.189057/1‑150 (MQ=60)
                                              TGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGC                                                                                                         >  SRR19973063.3190211/1‑150 (MQ=60)
                                              TGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGC                                                                                                         <  SRR19973063.1510179/150‑1 (MQ=60)
                                              TGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGC                                                                                                         <  SRR19973063.1510310/150‑1 (MQ=60)
                                                AAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCANTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTC                                                                                                       >  SRR19973063.2689657/1‑150 (MQ=60)
                                                AAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTC                                                                                                       <  SRR19973063.2441842/150‑1 (MQ=60)
                                                 AACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCG                                                                                                      <  SRR19973063.2235112/150‑1 (MQ=60)
                                                 AACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCG                                                                                                      <  SRR19973063.2235132/150‑1 (MQ=60)
                                                 AACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCG                                                                                                      <  SRR19973063.5145945/150‑1 (MQ=60)
                                                  ACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTCTTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTACCGCGCTGAACCTGCTCGG                                                                                                     >  SRR19973063.2720754/1‑150 (MQ=60)
                                                   CAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGC                                                                                                    <  SRR19973063.1883093/150‑1 (MQ=60)
                                                     AGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCT                                                                                                  >  SRR19973063.4217181/1‑150 (MQ=60)
                                                         CTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCA                                                                                              >  SRR19973063.4863687/1‑150 (MQ=60)
                                                           GGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACA                                                                                            >  SRR19973063.2998622/1‑150 (MQ=60)
                                                           GGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACA                                                                                            >  SRR19973063.2998640/1‑150 (MQ=60)
                                                           GGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACA                                                                                            >  SRR19973063.3778813/1‑150 (MQ=60)
                                                           GGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTACTCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTAAACCTGCACGGCCTTGCACA                                                                                            >  SRR19973063.1189637/1‑150 (MQ=60)
                                                              CGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACA                                                                                         <  SRR19973063.4508950/150‑1 (MQ=60)
                                                                TTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAG                                                                                       >  SRR19973063.2290129/1‑150 (MQ=60)
                                                                 TGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGA                                                                                      >  SRR19973063.3654383/1‑150 (MQ=60)
                                                                    CGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCT                                                                                   >  SRR19973063.4405884/1‑150 (MQ=60)
                                                                    CGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCT                                                                                   >  SRR19973063.4406029/1‑150 (MQ=60)
                                                                    CGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCT                                                                                   >  SRR19973063.1196714/1‑150 (MQ=60)
                                                                    CGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCT                                                                                   >  SRR19973063.1193377/1‑150 (MQ=60)
                                                                      CAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCG                                                                                 <  SRR19973063.1518792/150‑1 (MQ=60)
                                                                      CAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCG                                                                                 <  SRR19973063.4242107/150‑1 (MQ=60)
                                                                      CAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCG                                                                                 <  SRR19973063.1518418/150‑1 (MQ=60)
                                                                       AGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGA                                                                                >  SRR19973063.3211618/1‑150 (MQ=60)
                                                                       AGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGA                                                                                >  SRR19973063.2942261/1‑150 (MQ=60)
                                                                       AGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGA                                                                                >  SRR19973063.2337625/1‑150 (MQ=60)
                                                                        GCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGAC                                                                               <  SRR19973063.3295161/150‑1 (MQ=60)
                                                                         CATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACG                                                                              <  SRR19973063.465841/150‑1 (MQ=60)
                                                                           TTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAA                                                                            >  SRR19973063.2225798/1‑150 (MQ=60)
                                                                              AAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGC                                                                         >  SRR19973063.5129928/1‑150 (MQ=60)
                                                                                AGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAA                                                                       >  SRR19973063.4847352/1‑150 (MQ=60)
                                                                                    CTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCA                                                                   <  SRR19973063.1505176/150‑1 (MQ=60)
                                                                                    CTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCA                                                                   >  SRR19973063.1301088/1‑150 (MQ=60)
                                                                                      GCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACA                                                                 <  SRR19973063.2969445/150‑1 (MQ=60)
                                                                                        GTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAA                                                               >  SRR19973063.5054358/1‑150 (MQ=60)
                                                                                         TGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGAACAACAAGATCTCGACGAAGGCAAATCACAAAT                                                              >  SRR19973063.2724506/1‑150 (MQ=60)
                                                                                          GCTGAAAACATCTCAACCAAAGTCTTTAATATTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAAACACAAATC                                                             >  SRR19973063.901240/1‑150 (MQ=60)
                                                                                           CTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCG                                                            >  SRR19973063.1833438/1‑150 (MQ=60)
                                                                                           CTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCG                                                            >  SRR19973063.1833434/1‑150 (MQ=60)
                                                                                            TGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGC                                                           >  SRR19973063.2392137/1‑150 (MQ=60)
                                                                                                AACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCAC                                                       <  SRR19973063.122792/150‑1 (MQ=60)
                                                                                                AACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCAC                                                       <  SRR19973063.122751/150‑1 (MQ=60)
                                                                                                 ACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACG                                                      >  SRR19973063.2318510/1‑150 (MQ=60)
                                                                                                  CATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGG                                                     >  SRR19973063.126386/1‑150 (MQ=60)
                                                                                                    TCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCG                                                   <  SRR19973063.1620219/150‑1 (MQ=60)
                                                                                                    TCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCG                                                   <  SRR19973063.1620222/150‑1 (MQ=60)
                                                                                                    TCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTATTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCG                                                   <  SRR19973063.1620053/150‑1 (MQ=60)
                                                                                                       CAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAA                                                >  SRR19973063.4538195/1‑150 (MQ=60)
                                                                                                         ACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACC                                              >  SRR19973063.4634782/1‑150 (MQ=60)
                                                                                                         ACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACC                                              <  SRR19973063.2929271/150‑1 (MQ=60)
                                                                                                         ACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACC                                              >  SRR19973063.1664822/1‑150 (MQ=60)
                                                                                                         ACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACC                                              <  SRR19973063.38549/150‑1 (MQ=60)
                                                                                                          CCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCG                                             >  SRR19973063.3780176/1‑150 (MQ=60)
                                                                                                            AAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTG                                           >  SRR19973063.581558/1‑150 (MQ=60)
                                                                                                            AAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTG                                           <  SRR19973063.2852939/150‑1 (MQ=60)
                                                                                                            AAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTG                                           <  SRR19973063.1862237/150‑1 (MQ=60)
                                                                                                            AAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTG                                           >  SRR19973063.1384772/1‑150 (MQ=60)
                                                                                                              AGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCG                                         >  SRR19973063.3124755/1‑150 (MQ=60)
                                                                                                              AGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCG                                         >  SRR19973063.3124778/1‑150 (MQ=60)
                                                                                                                   TTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAA                                    >  SRR19973063.5020893/1‑150 (MQ=60)
                                                                                                                   TTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAA                                    >  SRR19973063.3491119/1‑150 (MQ=60)
                                                                                                                      ATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCG                                 >  SRR19973063.365872/1‑150 (MQ=60)
                                                                                                                         GTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCA                              <  SRR19973063.4182454/150‑1 (MQ=60)
                                                                                                                          TGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAA                             <  SRR19973063.4896628/150‑1 (MQ=60)
                                                                                                                          TGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAA                             <  SRR19973063.4896640/150‑1 (MQ=60)
                                                                                                                            GATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATA                           <  SRR19973063.4452949/150‑1 (MQ=60)
                                                                                                                            GATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATAGCTCACGGAGAAACCGTGCGTGAAACCGCCAATA                           >  SRR19973063.634355/1‑150 (MQ=60)
                                                                                                                             ATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATAT                          >  SRR19973063.926811/1‑150 (MQ=60)
                                                                                                                             ATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATAT                          >  SRR19973063.4702782/1‑150 (MQ=60)
                                                                                                                             ATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATAT                          >  SRR19973063.559620/1‑150 (MQ=60)
                                                                                                                             ATTAGGTATTTCCGTAGTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATAT                          <  SRR19973063.4288611/150‑1 (MQ=60)
                                                                                                                              TTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAAACGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATG                         >  SRR19973063.395613/1‑150 (MQ=60)
                                                                                                                                  GTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCT                     >  SRR19973063.1143652/1‑150 (MQ=60)
                                                                                                                                    ATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCC                   <  SRR19973063.2403514/150‑1 (MQ=60)
                                                                                                                                    ATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCC                   <  SRR19973063.2403450/150‑1 (MQ=60)
                                                                                                                                       TCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTC                <  SRR19973063.35501/150‑1 (MQ=60)
                                                                                                                                        CCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTGGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCT               <  SRR19973063.3996480/150‑1 (MQ=60)
                                                                                                                                         CGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTG              <  SRR19973063.4525608/150‑1 (MQ=60)
                                                                                                                                         CGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTG              >  SRR19973063.4194988/1‑150 (MQ=60)
                                                                                                                                          GTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTGC             >  SRR19973063.3756622/1‑150 (MQ=60)
                                                                                                                                          GTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTGC             >  SRR19973063.4695340/1‑150 (MQ=60)
                                                                                                                                          GTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTGC             >  SRR19973063.2334525/1‑150 (MQ=60)
                                                                                                                                           TATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTGCG            >  SRR19973063.2917151/1‑150 (MQ=60)
                                                                                                                                           TATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTGCG            <  SRR19973063.1111702/150‑1 (MQ=60)
                                                                                                                                           TATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTGCG            <  SRR19973063.1111782/150‑1 (MQ=60)
                                                                                                                                           TATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTGCG            >  SRR19973063.1751700/1‑150 (MQ=60)
                                                                                                                                           TATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTGCG            >  SRR19973063.3502162/1‑150 (MQ=60)
                                                                                                                                           TATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTGCG            >  SRR19973063.3502163/1‑150 (MQ=60)
                                                                                                                                           TATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTGCG            >  SRR19973063.3502229/1‑150 (MQ=60)
                                                                                                                                            ATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTGCGC           >  SRR19973063.1031331/1‑150 (MQ=60)
                                                                                                                                             TTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTGCGCC          >  SRR19973063.865856/1‑150 (MQ=60)
                                                                                                                                             TTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTGCGCC          >  SRR19973063.10237/1‑150 (MQ=60)
                                                                                                                                                    AAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTGCGCCGACGCTT   <  SRR19973063.1759317/150‑1 (MQ=60)
                                                                                                                                                     AAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTGCGCCGACGCTAT  <  SRR19973063.4347844/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
CACGAGAAAGATTTTTTGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGACAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTGCGCCGACGCTAT  >  CP009273/2999678‑2999976

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑