Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,022,562 C→T 93.2% G577R (GGA→AGA)  ygfT ← putative oxidoreductase, Fe‑S subunit/nucleotide‑binding subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,022,5620CT93.2% 511.8 / 23.0 162G577R (GGA→AGA) ygfTputative oxidoreductase, Fe‑S subunit/nucleotide‑binding subunit
Reads supporting (aligned to +/- strand):  ref base C (5/6);  new base T (89/62);  total (94/68)
Fisher's exact test for biased strand distribution p-value = 5.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 162 total aligned reads displayed.

TGCCTTCCTGCGGCCATTGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCCGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGACCATCCGGCCCCGGCTCACCCATGGCGGTACG  >  CP009273/3022417‑3022703
                                                                                                                                                 |                                                                                                                                             
tgcctTCCTGCGGCCATTGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCCGc                                                                                                                                             <  2:1575382/148‑1 (MQ=255)
tgcctTCCTGCGGCCATTGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCCGc                                                                                                                                             <  2:1575330/148‑1 (MQ=255)
 gcctTCCTGCGGCCATTGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCt                                                                                                                                            >  1:2825577/1‑148 (MQ=255)
   ctTCCTGCGGCCATTGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCCGCTGc                                                                                                                                          >  2:1972303/1‑148 (MQ=255)
    ttCCTGCGGCCATTGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGcc                                                                                                                                         <  1:2942084/148‑1 (MQ=255)
    ttCCTGCGGCCATTGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGcc                                                                                                                                         <  2:1334429/148‑1 (MQ=255)
     tCCTGCGGCCATTGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGccc                                                                                                                                        <  2:598060/148‑1 (MQ=255)
        tGCGGCCATTGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGc                                                                                                                                     >  2:2091101/1‑148 (MQ=255)
         gCGGCCATTGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCa                                                                                                                                    >  2:50232/1‑148 (MQ=255)
            gCCATTGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAAcc                                                                                                                                 >  1:190410/1‑148 (MQ=255)
             ccATTGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCa                                                                                                                                >  1:211570/1‑148 (MQ=255)
                ttGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACgg                                                                                                                             >  1:2785845/1‑148 (MQ=255)
                ttGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACgg                                                                                                                             >  1:2785512/1‑148 (MQ=255)
                 tGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACggc                                                                                                                            >  2:3187393/1‑148 (MQ=255)
                    aGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACggcatg                                                                                                                         <  1:1249665/148‑1 (MQ=255)
                           aCCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCCGCTGCCCTGCAACCACGGCATGGCATgcg                                                                                                                  >  1:1005624/1‑148 (MQ=255)
                               gATCCGCGCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTg                                                                                                              >  1:2962825/1‑148 (MQ=255)
                               gATCCGCGCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTg                                                                                                              >  1:293236/1‑148 (MQ=255)
                                aTCCGCGCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTgg                                                                                                             >  1:1405667/1‑148 (MQ=255)
                                aTCCGCGCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTgg                                                                                                             >  2:2822822/1‑148 (MQ=255)
                                  ccGCGCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGaa                                                                                                           <  2:2417434/148‑1 (MQ=255)
                                      gcCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCa                                                                                                       <  1:2634343/148‑1 (MQ=255)
                                       ccATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCaa                                                                                                      >  2:268435/1‑148 (MQ=255)
                                        cATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCaaa                                                                                                     >  1:339433/1‑148 (MQ=255)
                                         aTGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCCGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAg                                                                                                    >  2:330058/1‑148 (MQ=255)
                                          tGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAgg                                                                                                   >  1:152873/1‑148 (MQ=255)
                                          tGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAgg                                                                                                   <  1:2308407/148‑1 (MQ=255)
                                              cTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCAt                                                                                               >  1:708322/1‑148 (MQ=255)
                                               tGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATa                                                                                              <  2:537161/148‑1 (MQ=255)
                                               tGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATa                                                                                              <  1:494222/148‑1 (MQ=255)
                                                 cATCACCACCATCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAAt                                                                                            <  2:2072706/148‑1 (MQ=255)
                                                      ccaccaGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATaa                                                                                             >  1:1485920/1‑142 (MQ=255)
                                                        accaGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACa                                                                                     >  1:1569510/1‑148 (MQ=255)
                                                         ccaGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACAt                                                                                    <  2:1817456/148‑1 (MQ=255)
                                                         ccaGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACAt                                                                                    <  1:3064549/148‑1 (MQ=255)
                                                         ccaGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACAt                                                                                    <  1:3077936/148‑1 (MQ=255)
                                                         ccaGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACAt                                                                                    <  2:1181155/148‑1 (MQ=255)
                                                          caGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATc                                                                                   >  1:546296/1‑148 (MQ=255)
                                                          caGCAAAGACATTTTTCAGATTCGTCTGGGTAGGTAAATACCCGACGTCACCGTTTTGAATCAGGCCCCATTTATAGAGTTTAATTCTTCAGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACAAAAGGCCATCATCAGAACATc                                                                                   >  2:1999577/1‑148 (MQ=255)
                                                               aaGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATcggcgg                                                                              >  1:2542903/1‑148 (MQ=255)
                                                                aGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGACACCAACGGCCATAATCAGAACATCGGCggg                                                                             >  2:2919419/1‑148 (MQ=255)
                                                                aGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCggg                                                                             <  2:3054867/148‑1 (MQ=255)
                                                                aGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCggg                                                                             >  2:2920684/1‑148 (MQ=255)
                                                                 gACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGc                                                                            >  1:2212555/1‑148 (MQ=255)
                                                                 gACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGc                                                                            >  1:1676289/1‑148 (MQ=255)
                                                                  aCTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCCAAGGCCATAATCAGAACATCGGCGGGCa                                                                           >  2:2739873/1‑148 (MQ=255)
                                                                   cTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCaa                                                                          >  2:666058/1‑148 (MQ=255)
                                                                           aGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAAct                                                                  <  1:463740/148‑1 (MQ=255)
                                                                             aTGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAActct                                                                >  1:534979/1‑148 (MQ=255)
                                                                             aTGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAActct                                                                >  1:2117585/1‑148 (MQ=255)
                                                                               gCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGa                                                              <  2:3298506/148‑1 (MQ=255)
                                                                                cGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGaa                                                             >  2:3078963/1‑148 (MQ=255)
                                                                                cGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATGTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGaa                                                             <  2:2781765/148‑1 (MQ=255)
                                                                                cGTCTGGGTAGGTAAATACCCGACGTCACCGGTGTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGaa                                                             <  2:1894298/148‑1 (MQ=255)
                                                                                  tCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAAcc                                                           <  2:1114606/148‑1 (MQ=255)
                                                                                   cTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAAccc                                                          >  2:631344/1‑148 (MQ=255)
                                                                                   cTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAAccc                                                          >  2:631577/1‑148 (MQ=255)
                                                                                     gggTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCTATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGc                                                        >  1:249653/1‑148 (MQ=255)
                                                                                     gggTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGc                                                        >  2:2824905/1‑148 (MQ=255)
                                                                                     gggTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGc                                                        >  1:2543571/1‑148 (MQ=255)
                                                                                     gggTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGc                                                        <  1:3187393/148‑1 (MQ=255)
                                                                                      ggtaggtaAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCt                                                       >  2:3093845/1‑148 (MQ=255)
                                                                                      ggtaggtaAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCt                                                       >  1:259320/1‑148 (MQ=255)
                                                                                           gtaAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCgg                                                  >  2:940059/1‑148 (MQ=255)
                                                                                           gtaAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTGATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCgg                                                  <  1:195564/148‑1 (MQ=255)
                                                                                             aaaTACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCggac                                                >  2:1234278/1‑148 (MQ=255)
                                                                                                 aCCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCggacgagg                                            <  2:3223322/148‑1 (MQ=255)
                                                                                                 aCCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCggacgagg                                            <  2:3223429/148‑1 (MQ=255)
                                                                                                      aCGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGgacgac                                       <  2:1405667/148‑1 (MQ=255)
                                                                                                      aCGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGgacgac                                       >  2:82258/1‑148 (MQ=255)
                                                                                                      aCGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGgacgac                                       >  1:613277/1‑148 (MQ=255)
                                                                                                      aCGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGgacgac                                       >  1:1428460/1‑148 (MQ=255)
                                                                                                          cACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAACGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCg                                   >  2:2774651/1‑148 (MQ=255)
                                                                                                          cACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAACGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCg                                   >  2:353561/1‑148 (MQ=255)
                                                                                                          cACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACCTCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCg                                   >  2:2774349/1‑148 (MQ=255)
                                                                                                          cACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCg                                   >  1:565486/1‑148 (MQ=255)
                                                                                                           aCCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGa                                  >  1:2273646/1‑148 (MQ=255)
                                                                                                           aCCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCCGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGa                                  >  2:1441472/1‑148 (MQ=255)
                                                                                                             cGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGAcc                                <  1:956511/148‑1 (MQ=255)
                                                                                                             cGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGAcc                                <  1:73819/148‑1 (MQ=255)
                                                                                                             cGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGAcc                                <  1:73760/148‑1 (MQ=255)
                                                                                                                     aTCTGGCCCCTTTTATCGAGTTTAATTCTGCTGCCCTGCAACCTCGGCATGGCATGCGCCTGGAAACCAAAGGCCATATTCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGACCATCCGGcc                        <  1:1688639/148‑1 (MQ=255)
                                                                                                                      tCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGACCATCCGGccc                       >  2:204855/1‑148 (MQ=255)
                                                                                                                              cATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGACCATCCGGCCCCGGCTCAc               >  2:778057/1‑148 (MQ=255)
                                                                                                                               aTTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCTGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGACCATCCGGCCCCGGCTCAcc              >  1:1241441/1‑148 (MQ=255)
                                                                                                                               aTTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCTGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGACCATCCGGCCCCGGCTCAcc              >  1:1240153/1‑148 (MQ=255)
                                                                                                                               aTTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGACCATCCGGCCCCGGCTCAcc              >  1:1077600/1‑148 (MQ=255)
                                                                                                                               aTTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGACCATCCGGCCCCGGCTCAcc              >  2:2370412/1‑148 (MQ=255)
                                                                                                                                tttATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGACCATCCGGCCCCGGCTCAccc             >  1:1803900/1‑148 (MQ=255)
                                                                                                                                tttATCGAGTTTAATTCCGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGACCATCCGGCCCCGGCTCAccc             <  2:2499488/148‑1 (MQ=255)
                                                                                                                                 ttATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGACCATCCGGCCCCGGCTCACCCa            >  2:2213800/1‑148 (MQ=255)
                                                                                                                                 ttATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGACCATCCGGCCCCGGCTCACCCa            >  2:2214056/1‑148 (MQ=255)
                                                                                                                                 ttATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGACCATCCGGCCCCGGCTCACCCa            <  2:259056/148‑1 (MQ=255)
                                                                                                                                 ttATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGACCATCCGGCCCCGGCTCACCCa            <  2:259320/148‑1 (MQ=255)
                                                                                                                                 ttATCGAGTTTAATTCTGCTGCCATGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGACCATCCGGCCCCGGCTCACCCa            >  1:3276851/1‑148 (MQ=255)
                                                                                                                                    tCGAGTTTAATTCCGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGACCATCCGGCCCCGGCTCACCCATgg         >  2:1795401/1‑148 (MQ=255)
                                                                                                                                       aGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGACCATCCGGCCCCGGCTCACCCATGGCgg      <  2:700595/148‑1 (MQ=255)
                                                                                                                                       aGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGACCATCCGGCCCCGGCTCACCCATGGCgg      <  2:2737746/148‑1 (MQ=255)
                                                                                                                                          ttAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGACCATCCGGCCCCGGCTCACCCATGGCGGTAc   >  2:1678947/1‑148 (MQ=255)
                                                                                                                                           tAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGACCATCCGGCCCCGGCTCACCCATGGCGGTACg  <  2:869896/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                             
TGCCTTCCTGCGGCCATTGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCCGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGACCATCCGGCCCCGGCTCACCCATGGCGGTACG  >  CP009273/3022417‑3022703

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCCTGCCTTCCTGCGGCCATTGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCCGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGACCATCCGGCCCCGGCTCACCCA  >  CP009273/3022413‑3022693
                                                                                                                                                     |                                                                                                                                   
CGCCTGCCTTCCTGCGGCCATTGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCT                                                                                                                                     <  SRR19973070.2997621/150‑1 (MQ=60)
CGCCTGCCTTCCTGCGGCCATTGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCT                                                                                                                                     <  SRR19973070.2997619/150‑1 (MQ=60)
CGCCTGCCTTCCTGCGGCCATTGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCC                                                                                                                                     <  SRR19973070.811846/150‑1 (MQ=60)
CGCCTGCCTTCCTGCGGCCATTGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCC                                                                                                                                     <  SRR19973070.3037439/150‑1 (MQ=60)
   CTGCCTTCCTGCGGCCATTGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCT                                                                                                                                  >  SRR19973070.540762/1‑150 (MQ=60)
   CTGCCTTCCTGCGGCCATTGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCT                                                                                                                                  >  SRR19973070.2534133/1‑150 (MQ=60)
   CTGCCTTCCTGCGGCCATTGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCT                                                                                                                                  >  SRR19973070.2843861/1‑150 (MQ=60)
        TTCCTGCGGCCATTGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCT                                                                                                                             <  SRR19973070.1545320/150‑1 (MQ=60)
        TTCCTGCGGCCATTGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCT                                                                                                                             <  SRR19973070.2961044/150‑1 (MQ=60)
              CGGCCATTGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACC                                                                                                                       >  SRR19973070.191879/1‑150 (MQ=60)
               GGCCATTGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCA                                                                                                                      >  SRR19973070.213567/1‑150 (MQ=60)
               GGCCATTGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCA                                                                                                                      >  SRR19973070.213133/1‑150 (MQ=60)
                  CATTGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTCGGTAAATACACGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGC                                                                                                                   >  SRR19973070.2803765/1‑150 (MQ=60)
                  CATTGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGG                                                                                                                   >  SRR19973070.2803773/1‑150 (MQ=60)
                  CATTGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGG                                                                                                                   >  SRR19973070.2803434/1‑150 (MQ=60)
                      GCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATG                                                                                                               <  SRR19973070.1461227/150‑1 (MQ=60)
                      GCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATG                                                                                                               <  SRR19973070.3113841/150‑1 (MQ=60)
                        AGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGC                                                                                                             <  SRR19973070.1258450/150‑1 (MQ=60)
                        AGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGC                                                                                                             <  SRR19973070.1258429/150‑1 (MQ=60)
                        AGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGC                                                                                                             >  SRR19973070.2755370/1‑150 (MQ=60)
                             CAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCCGCTGCCCTGCAACCACGGCATGGCATGCG                                                                                                        >  SRR19973070.1012790/1‑150 (MQ=60)
                                 CAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTG                                                                                                    >  SRR19973070.2981873/1‑150 (MQ=60)
                                 CAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTG                                                                                                    >  SRR19973070.2981815/1‑150 (MQ=60)
                                 CAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTG                                                                                                    >  SRR19973070.295246/1‑150 (MQ=60)
                                 CAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTG                                                                                                    >  SRR19973070.295234/1‑150 (MQ=60)
                                  AGATCCGCGCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGG                                                                                                   >  SRR19973070.1415205/1‑150 (MQ=60)
                                          GCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAA                                                                                           >  SRR19973070.341909/1‑150 (MQ=60)
                                          GCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAA                                                                                           <  SRR19973070.2651521/150‑1 (MQ=60)
                                            CATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGG                                                                                         >  SRR19973070.154154/1‑150 (MQ=60)
                                              TGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCC                                                                                       <  SRR19973070.2323756/150‑1 (MQ=60)
                                                AACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCAT                                                                                     >  SRR19973070.713377/1‑150 (MQ=60)
                                                   TGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAAT                                                                                  <  SRR19973070.497674/150‑1 (MQ=60)
                                                   TGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCCGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAAT                                                                                  <  SRR19973070.2107166/150‑1 (MQ=60)
                                                     CATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCA                                                                                <  SRR19973070.1099981/150‑1 (MQ=60)
                                                        CACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAT                                                                             >  SRR19973070.1496318/1‑150 (MQ=60)
                                                          CCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACA                                                                           >  SRR19973070.1580540/1‑150 (MQ=60)
                                                            ACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATC                                                                         >  SRR19973070.549936/1‑150 (MQ=60)
                                                             CCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCG                                                                        <  SRR19973070.3097713/150‑1 (MQ=60)
                                                             CCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCG                                                                        <  SRR19973070.3084185/150‑1 (MQ=60)
                                                                 CAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGG                                                                    >  SRR19973070.2559532/1‑150 (MQ=60)
                                                                   AAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGC                                                                  >  SRR19973070.1687762/1‑150 (MQ=60)
                                                                   AAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGC                                                                  >  SRR19973070.2227101/1‑150 (MQ=60)
                                                                     GACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAA                                                                >  SRR19973070.3259812/1‑150 (MQ=60)
                                                                         TTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCA                                                            <  SRR19973070.2358283/150‑1 (MQ=60)
                                                                               AGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCT                                                      >  SRR19973070.538574/1‑150 (MQ=60)
                                                                               AGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCT                                                      <  SRR19973070.467051/150‑1 (MQ=60)
                                                                               AGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCT                                                      >  SRR19973070.2131776/1‑150 (MQ=60)
                                                                                       CTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCTATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGC                                              >  SRR19973070.251357/1‑150 (MQ=60)
                                                                                       CTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGC                                              >  SRR19973070.2560202/1‑150 (MQ=60)
                                                                                        TGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCT                                             >  SRR19973070.260793/1‑150 (MQ=60)
                                                                                        TGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCT                                             >  SRR19973070.261058/1‑150 (MQ=60)
                                                                                         GGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTA                                            <  SRR19973070.3208014/150‑1 (MQ=60)
                                                                                           GTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACC                                          <  SRR19973070.188150/150‑1 (MQ=60)
                                                                                               GTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTGATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGAC                                      <  SRR19973070.197063/150‑1 (MQ=60)
                                                                                                 AAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGA                                    >  SRR19973070.591352/1‑150 (MQ=60)
                                                                                                  AATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAG                                   >  SRR19973070.3065361/1‑150 (MQ=60)
                                                                                                        CGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGAC                             >  SRR19973070.617576/1‑150 (MQ=60)
                                                                                                        CGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGAC                             >  SRR19973070.414216/1‑150 (MQ=60)
                                                                                                        CGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGAC                             >  SRR19973070.1438319/1‑150 (MQ=60)
                                                                                                         GACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACG                            <  SRR19973070.1599786/150‑1 (MQ=60)
                                                                                                            GTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCG                         >  SRR19973070.569278/1‑150 (MQ=60)
                                                                                                             TCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGA                        >  SRR19973070.2288749/1‑150 (MQ=60)
                                                                                                             TCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGA                        >  SRR19973070.625992/1‑150 (MQ=60)
                                                                                                                 CGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGACCAT                    <  SRR19973070.74616/150‑1 (MQ=60)
                                                                                                                 CGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGACCAT                    <  SRR19973070.74612/150‑1 (MQ=60)
                                                                                                                 CGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGACCAT                    <  SRR19973070.74557/150‑1 (MQ=60)
                                                                                                                 CGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGACCAT                    <  SRR19973070.963337/150‑1 (MQ=60)
                                                                                                                 CGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGACCAT                    <  SRR19973070.2227354/150‑1 (MQ=60)
                                                                                                                   GTTTGAATCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGACCATCC                  >  SRR19973070.1743387/1‑150 (MQ=60)
                                                                                                                         ATCTGGCCCCTTTTATCGAGTTTAATTCTGCTGCCCTGCAACCTCGGCATGGCATGCGCCTGGAAACCAAAGGCCATATTCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGACCATCCGGCCCC            <  SRR19973070.1700241/150‑1 (MQ=60)
                                                                                                                          TCAGGCCCCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGACCATCCGGCCCCG           <  SRR19973070.50846/150‑1 (MQ=60)
                                                                                                                                 CCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCTGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGACCATCCGGCCCCGGCTCACC    >  SRR19973070.1250149/1‑150 (MQ=60)
                                                                                                                                 CCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCTGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGACCATCCGGCCCCGGCTCACC    >  SRR19973070.1250141/1‑150 (MQ=60)
                                                                                                                                 CCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCTGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGACCATCCGGCCCCGGCTCACC    >  SRR19973070.1248841/1‑150 (MQ=60)
                                                                                                                                 CCATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGACCATCCGGCCCCGGCTCACC    >  SRR19973070.1085102/1‑150 (MQ=60)
                                                                                                                                  CATTTATCGAGTTTAATTCTGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGACCATCCGGCCCCGGCTCACCC   >  SRR19973070.1816349/1‑150 (MQ=60)
                                                                                                                                   ATTTATCGAGTTTAATTCTGCTGCCATGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGACCATCCGGCCCCGGCTCACCCA  >  SRR19973070.3297908/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                   
CGCCTGCCTTCCTGCGGCCATTGCAGTGACAACCAGATCCGCGCCATGAACTGCATCACCACCAGCAAAGACTTTTTTCAGATGCGTCTGGGTAGGTAAATACCCGACGTCACCGGTTTGAATCAGGCCCCATTTATCGAGTTTAATTCCGCTGCCCTGCAACCACGGCATGGCATGCGCCTGGAAACCAAAGGCCATAATCAGAACATCGGCGGGCAATTCAAACTCTGAACCCGCTACCGGACGAGGACGACGGCGACCATCCGGCCCCGGCTCACCCA  >  CP009273/3022413‑3022693

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑