Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,039,703 C→A 87.2% A900S (GCG→TCG)  gcvP ← glycine decarboxylase, PLP‑dependent, subunit (protein P) of glycine cleavage complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,039,7030CA87.2% 349.9 / 53.7 148A900S (GCG→TCG) gcvPglycine decarboxylase, PLP‑dependent, subunit (protein P) of glycine cleavage complex
Reads supporting (aligned to +/- strand):  ref base C (13/6);  new base A (48/81);  total (61/87)
Fisher's exact test for biased strand distribution p-value = 1.27e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

Only 100 of 148 total aligned reads displayed.

AGAACAGGTTACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGCGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTCAACCATCAGCGTA  >  CP009273/3039559‑3039842
                                                                                                                                                |                                                                                                                                           
aGAACAGGTTACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGtt                                                                                                                                          <  2:3221237/148‑1 (MQ=255)
  aaCAGGTTACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCa                                                                                                                                        >  1:1904362/1‑148 (MQ=255)
  aaCAGGTTACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCa                                                                                                                                        >  2:2769294/1‑148 (MQ=255)
   aCAGGTTACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCAc                                                                                                                                       <  2:1258344/148‑1 (MQ=255)
   aCAGGTTACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCAc                                                                                                                                       <  2:1258372/148‑1 (MQ=255)
     aGGTTACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCa                                                                                                                                     >  1:1442967/1‑148 (MQ=255)
           cGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGCGTTCACCAGCGGGt                                                                                                                               >  1:2841479/1‑148 (MQ=255)
             gTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTa                                                                                                                             >  1:51968/1‑148 (MQ=255)
                  cGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGAGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTc                                                                                                                        <  1:705046/148‑1 (MQ=255)
                     aaaCATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAg                                                                                                                     <  2:1790988/148‑1 (MQ=255)
                       aCATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCg                                                                                                                   >  1:2385457/1‑148 (MQ=255)
                       aCATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCg                                                                                                                   >  1:2385531/1‑148 (MQ=255)
                       aCATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCg                                                                                                                   >  1:2965253/1‑148 (MQ=255)
                        catcatCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGGTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCgg                                                                                                                  >  2:3083129/1‑148 (MQ=255)
                          tcatcCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGcc                                                                                                                >  1:3239633/1‑148 (MQ=255)
                           catcCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCa                                                                                                               >  2:593295/1‑148 (MQ=255)
                           catcCAGACGTTTAACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCa                                                                                                               >  1:246781/1‑148 (MQ=255)
                             tcCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGa                                                                                                             <  1:2328153/148‑1 (MQ=255)
                               cAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGaca                                                                                                           <  1:2263067/148‑1 (MQ=255)
                               cAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGaca                                                                                                           <  1:553264/148‑1 (MQ=255)
                                 gACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACAcc                                                                                                         >  2:1037006/1‑148 (MQ=255)
                                 gACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACAcc                                                                                                         >  1:780813/1‑148 (MQ=255)
                                     tttCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCt                                                                                                     <  2:1352762/148‑1 (MQ=255)
                                        cACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTc                                                                                                  <  2:1340584/148‑1 (MQ=255)
                                        cACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTc                                                                                                  <  1:2601446/148‑1 (MQ=255)
                                         aCTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCa                                                                                                 <  2:1365941/148‑1 (MQ=255)
                                         aCTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCa                                                                                                 <  2:2201325/148‑1 (MQ=255)
                                         aCTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCa                                                                                                 <  2:2201322/148‑1 (MQ=255)
                                          cTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCAc                                                                                                <  1:2375083/148‑1 (MQ=255)
                                              cGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTgg                                                                                            <  2:1589428/148‑1 (MQ=255)
                                               ggCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGt                                                                                           <  2:3133518/148‑1 (MQ=255)
                                               ggCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGt                                                                                           >  1:2579029/1‑148 (MQ=255)
                                                gCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTc                                                                                          >  1:2162046/1‑148 (MQ=255)
                                                 ccAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCa                                                                                         <  1:1456715/148‑1 (MQ=255)
                                                  cAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGCGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCaa                                                                                        <  2:2322340/148‑1 (MQ=255)
                                                  cAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCaa                                                                                        <  2:3183700/148‑1 (MQ=255)
                                                   aGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGCGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAAt                                                                                       >  2:1993029/1‑148 (MQ=255)
                                                   aGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAAt                                                                                       <  1:1034429/148‑1 (MQ=255)
                                                       tttGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCt                                                                                   <  1:2769294/148‑1 (MQ=255)
                                                        ttGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGCGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTg                                                                                  >  1:515950/1‑148 (MQ=255)
                                                        ttGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTg                                                                                  <  2:7323/148‑1 (MQ=255)
                                                           tCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGCGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTgcgc                                                                               >  1:2984968/1‑148 (MQ=255)
                                                           tCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGCGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTgcgc                                                                               >  1:2985131/1‑148 (MQ=255)
                                                             tGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCgg                                                                             <  2:735230/148‑1 (MQ=255)
                                                              gCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGa                                                                            <  2:1582408/148‑1 (MQ=255)
                                                              gCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGa                                                                            <  2:2060888/148‑1 (MQ=255)
                                                                 acacCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCTAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAg                                                                         <  1:973653/148‑1 (MQ=255)
                                                                 acacCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAg                                                                         >  2:2876753/1‑148 (MQ=255)
                                                                   acCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGCGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGcc                                                                       <  2:1528396/148‑1 (MQ=255)
                                                                   acCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGcc                                                                       >  1:2727908/1‑148 (MQ=255)
                                                                   acCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGcc                                                                       <  1:2672680/148‑1 (MQ=255)
                                                                    ccTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCa                                                                      >  1:1007824/1‑148 (MQ=255)
                                                                    ccTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCa                                                                      >  1:1007842/1‑148 (MQ=255)
                                                                          gggAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATcg                                                                >  2:795548/1‑148 (MQ=255)
                                                                             aaTACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGt                                                             <  2:855438/148‑1 (MQ=255)
                                                                               tACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCg                                                           >  2:520591/1‑148 (MQ=255)
                                                                                aCCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGa                                                          >  2:146389/1‑148 (MQ=255)
                                                                                 ccGCAACTTTACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGCGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGAt                                                         >  1:1071347/1‑148 (MQ=255)
                                                                                 ccGCAACTTTACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGCGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGAt                                                         >  1:1071618/1‑148 (MQ=255)
                                                                                   gCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATaa                                                       <  2:1169681/148‑1 (MQ=255)
                                                                                    cAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATaaa                                                      <  2:3066043/148‑1 (MQ=255)
                                                                                       cTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCg                                                   >  1:2021882/1‑148 (MQ=255)
                                                                                        ttCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGa                                                  <  1:1600519/148‑1 (MQ=255)
                                                                                        ttCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGa                                                  <  1:450416/148‑1 (MQ=255)
                                                                                         tCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGAt                                                 <  1:2550496/148‑1 (MQ=255)
                                                                                          cACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATc                                                <  1:140064/148‑1 (MQ=255)
                                                                                            cGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCa                                              >  1:1353104/1‑148 (MQ=255)
                                                                                              gCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGCGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGt                                            <  2:1070850/148‑1 (MQ=255)
                                                                                                  tACGGATGCTCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCa                                        >  1:1218418/1‑148 (MQ=255)
                                                                                                  tACGGATGCTCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCa                                        >  1:1218911/1‑148 (MQ=255)
                                                                                                    cGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACt                                      <  1:719939/148‑1 (MQ=255)
                                                                                                    cGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACt                                      <  1:720059/148‑1 (MQ=255)
                                                                                                    cGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACt                                      <  2:495366/148‑1 (MQ=255)
                                                                                                       aTGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTg                                   >  1:75690/1‑148 (MQ=255)
                                                                                                        tGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGc                                  <  2:775584/148‑1 (MQ=255)
                                                                                                          cgcCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCtt                                <  2:12154/148‑1 (MQ=255)
                                                                                                           gcCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCttt                               <  2:3275746/148‑1 (MQ=255)
                                                                                                            cccACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTc                              >  1:1028043/1‑148 (MQ=255)
                                                                                                              cACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAg                            <  1:1508380/148‑1 (MQ=255)
                                                                                                              cACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAg                            <  1:784221/148‑1 (MQ=255)
                                                                                                               aCTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGa                           <  1:2644448/148‑1 (MQ=255)
                                                                                                               aCTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGa                           <  2:1807441/148‑1 (MQ=255)
                                                                                                                cTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGAt                          >  2:258142/1‑148 (MQ=255)
                                                                                                                 tCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGAtt                         <  2:1374715/148‑1 (MQ=255)
                                                                                                                  cGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTc                        <  1:1036921/148‑1 (MQ=255)
                                                                                                                   ggCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCa                       <  2:1224875/148‑1 (MQ=255)
                                                                                                                     cGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGt                     <  1:1920980/148‑1 (MQ=255)
                                                                                                                     cGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGt                     <  2:819346/148‑1 (MQ=255)
                                                                                                                       aCCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCg                   >  1:172366/1‑148 (MQ=255)
                                                                                                                       aCCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCg                   >  1:172386/1‑148 (MQ=255)
                                                                                                                          aGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGtt                <  2:1298463/148‑1 (MQ=255)
                                                                                                                          aGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGtt                <  2:1298415/148‑1 (MQ=255)
                                                                                                                          aGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGtt                <  1:958045/148‑1 (MQ=255)
                                                                                                                            ttCGCTCTGAATGTGCGGCGCGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTCa              >  2:318836/1‑148 (MQ=255)
                                                                                                                             tCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTCaa             <  2:2006931/148‑1 (MQ=255)
                                                                                                                                 tctGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTCAACCAt         >  1:1963988/1‑148 (MQ=255)
                                                                                                                                   tGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTCAACCATCa       <  1:3122071/148‑1 (MQ=255)
                                                                                                                                      aTGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTCAACCATCAGCg    <  1:2330594/148‑1 (MQ=255)
                                                                                                                                      aTGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTCAACCATCAGCg    <  1:670875/148‑1 (MQ=255)
                                                                                                                                        gtgCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTCAACCATCAGCGTa  >  2:1122765/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                           
AGAACAGGTTACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGCGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTCAACCATCAGCGTA  >  CP009273/3039559‑3039842

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GAGCAGAACAGGTTACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGCGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTCAACCATCAGCGTACCCGCCACCG  >  CP009273/3039555‑3039852
                                                                                                                                                    |                                                                                                                                                     
GAGCAGAACAGGTTACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAG                                                                                                                                                      <  SRR19973070.536814/150‑1 (MQ=60)
  GCAGAACAGGTTACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTT                                                                                                                                                    <  SRR19973070.3253696/150‑1 (MQ=60)
  GCAGAACAGGTTACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTT                                                                                                                                                    <  SRR19973070.1388061/150‑1 (MQ=60)
    AGAACAGGTTACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCA                                                                                                                                                  >  SRR19973070.1917243/1‑150 (MQ=60)
    AGAACAGGTTACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCA                                                                                                                                                  >  SRR19973070.1916493/1‑150 (MQ=60)
       ACAGGTTACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCA                                                                                                                                               >  SRR19973070.1452979/1‑150 (MQ=60)
             TACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGCGTTCACCAGCGGGT                                                                                                                                         >  SRR19973070.2859873/1‑150 (MQ=60)
               CGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTA                                                                                                                                       >  SRR19973070.52597/1‑150 (MQ=60)
                GGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGCGTTCACCAGCGGGTTAT                                                                                                                                      <  SRR19973070.584146/150‑1 (MQ=60)
                      CGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGAGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCA                                                                                                                                <  SRR19973070.710068/150‑1 (MQ=60)
                         AAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCG                                                                                                                             >  SRR19973070.2401211/1‑150 (MQ=60)
                         AAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCG                                                                                                                             >  SRR19973070.2401285/1‑150 (MQ=60)
                         AAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCG                                                                                                                             >  SRR19973070.2984317/1‑150 (MQ=60)
                            CATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCC                                                                                                                          >  SRR19973070.3260530/1‑150 (MQ=60)
                             ATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCA                                                                                                                         >  SRR19973070.1349794/1‑150 (MQ=60)
                             ATCATCCAGACGTTTAACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCA                                                                                                                         >  SRR19973070.248476/1‑150 (MQ=60)
                                ATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGCGTTCACCAGCGGGTTATCTTCCAGCGGCCAGAC                                                                                                                      >  SRR19973070.2055782/1‑150 (MQ=60)
                                 TCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACA                                                                                                                     <  SRR19973070.2343622/150‑1 (MQ=60)
                                   CAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACC                                                                                                                   >  SRR19973070.786263/1‑150 (MQ=60)
                                   CAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACC                                                                                                                   >  SRR19973070.2454129/1‑150 (MQ=60)
                                   CAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACC                                                                                                                   <  SRR19973070.2278084/150‑1 (MQ=60)
                                   CAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACC                                                                                                                   <  SRR19973070.556924/150‑1 (MQ=60)
                                            CACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCAC                                                                                                          <  SRR19973070.2618430/150‑1 (MQ=60)
                                              CTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCT                                                                                                        <  SRR19973070.2390782/150‑1 (MQ=60)
                                              CTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCT                                                                                                        <  SRR19973070.2355521/150‑1 (MQ=60)
                                                 TCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGT                                                                                                     >  SRR19973070.2595889/1‑150 (MQ=60)
                                                  CGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTC                                                                                                    >  SRR19973070.2176412/1‑150 (MQ=60)
                                                     CCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAAT                                                                                                 <  SRR19973070.1467656/150‑1 (MQ=60)
                                                     CCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAAT                                                                                                 <  SRR19973070.1466868/150‑1 (MQ=60)
                                                       AGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTT                                                                                               <  SRR19973070.1080893/150‑1 (MQ=60)
                                                       AGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTT                                                                                               <  SRR19973070.229501/150‑1 (MQ=60)
                                                       AGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTT                                                                                               <  SRR19973070.1041742/150‑1 (MQ=60)
                                                       AGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGATCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTT                                                                                               <  SRR19973070.1041741/150‑1 (MQ=60)
                                                        GTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTC                                                                                              >  SRR19973070.439787/1‑150 (MQ=60)
                                                          ATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGCGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTG                                                                                            >  SRR19973070.519482/1‑150 (MQ=60)
                                                          ATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGCGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTG                                                                                            >  SRR19973070.519523/1‑150 (MQ=60)
                                                           TTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGC                                                                                           <  SRR19973070.2787017/150‑1 (MQ=60)
                                                             TGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGCGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGC                                                                                         >  SRR19973070.3004110/1‑150 (MQ=60)
                                                             TGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGCGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGC                                                                                         >  SRR19973070.3004274/1‑150 (MQ=60)
                                                                     ACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCTAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCC                                                                                 <  SRR19973070.980597/150‑1 (MQ=60)
                                                                     ACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCC                                                                                 >  SRR19973070.2745500/1‑150 (MQ=60)
                                                                      CACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCA                                                                                >  SRR19973070.1014998/1‑150 (MQ=60)
                                                                      CACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCA                                                                                >  SRR19973070.1015016/1‑150 (MQ=60)
                                                                       ACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAG                                                                               <  SRR19973070.2690037/150‑1 (MQ=60)
                                                                                   TACCGCAACTTTACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGCGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGAT                                                                   >  SRR19973070.1078834/1‑150 (MQ=60)
                                                                                   TACCGCAACTTTACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGCGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGAT                                                                   >  SRR19973070.1079106/1‑150 (MQ=60)
                                                                                        CAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGCGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGC                                                              >  SRR19973070.759641/1‑150 (MQ=60)
                                                                                         AACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCG                                                             >  SRR19973070.2035558/1‑150 (MQ=60)
                                                                                            TTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATC                                                          <  SRR19973070.453662/150‑1 (MQ=60)
                                                                                            TTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATC                                                          <  SRR19973070.1611832/150‑1 (MQ=60)
                                                                                            TTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATC                                                          <  SRR19973070.1611697/150‑1 (MQ=60)
                                                                                             TCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCC                                                         >  SRR19973070.901189/1‑150 (MQ=60)
                                                                                             TCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCC                                                         >  SRR19973070.2567284/1‑150 (MQ=60)
                                                                                             TCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCC                                                         <  SRR19973070.2567178/150‑1 (MQ=60)
                                                                                              CACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCA                                                        >  SRR19973070.1362360/1‑150 (MQ=60)
                                                                                              CACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCA                                                        <  SRR19973070.141284/150‑1 (MQ=60)
                                                                                              CACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCA                                                        <  SRR19973070.141312/150‑1 (MQ=60)
                                                                                                    TGTACGGATGCTCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCA                                                  >  SRR19973070.1226958/1‑150 (MQ=60)
                                                                                                    TGTACGGATGCTCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCA                                                  >  SRR19973070.1227451/1‑150 (MQ=60)
                                                                                                     GTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCAC                                                 >  SRR19973070.299703/1‑150 (MQ=60)
                                                                                                      TACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACT                                                <  SRR19973070.2712734/150‑1 (MQ=60)
                                                                                                        CGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTT                                              <  SRR19973070.725066/150‑1 (MQ=60)
                                                                                                        CGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTT                                              <  SRR19973070.725187/150‑1 (MQ=60)
                                                                                                         GGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTG                                             >  SRR19973070.76499/1‑150 (MQ=60)
                                                                                                              CGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTC                                        >  SRR19973070.1035326/1‑150 (MQ=60)
                                                                                                              CGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTC                                        <  SRR19973070.3134872/150‑1 (MQ=60)
                                                                                                                  CACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGAT                                    <  SRR19973070.1518931/150‑1 (MQ=60)
                                                                                                                  CACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGAT                                    <  SRR19973070.789681/150‑1 (MQ=60)
                                                                                                                   ACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATT                                   <  SRR19973070.2661668/150‑1 (MQ=60)
                                                                                                                   ACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATT                                   <  SRR19973070.2661672/150‑1 (MQ=60)
                                                                                                                      CGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAG                                <  SRR19973070.1044333/150‑1 (MQ=60)
                                                                                                                      CGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAG                                <  SRR19973070.1044248/150‑1 (MQ=60)
                                                                                                                         CGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCG                             <  SRR19973070.1933936/150‑1 (MQ=60)
                                                                                                                         CGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCG                             <  SRR19973070.1934425/150‑1 (MQ=60)
                                                                                                                         CGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCG                             >  SRR19973070.173766/1‑150 (MQ=60)
                                                                                                                         CGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCG                             >  SRR19973070.173746/1‑150 (MQ=60)
                                                                                                                              AGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTCA                        <  SRR19973070.964881/150‑1 (MQ=60)
                                                                                                                                  CGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTCAACCA                    >  SRR19973070.718866/1‑150 (MQ=60)
                                                                                                                                   GCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTCAACCAT                   >  SRR19973070.1977241/1‑150 (MQ=60)
                                                                                                                                       TGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTCAACCATCAGC               <  SRR19973070.3142224/150‑1 (MQ=60)
                                                                                                                                          ATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTCAACCATCAGCGTA            <  SRR19973070.675665/150‑1 (MQ=60)
                                                                                                                                          ATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTCAACCATCAGCGTA            >  SRR19973070.282464/1‑150 (MQ=60)
                                                                                                                                          ATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTCAACCATCAGCGTA            <  SRR19973070.2346071/150‑1 (MQ=60)
                                                                                                                                                    AGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTCAACCATCAGCGTACCCGCCACCG  >  SRR19973070.1583214/1‑150 (MQ=60)
                                                                                                                                                    AGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTCAACCATCAGCGTACCCGCCACCG  >  SRR19973070.1583216/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
GAGCAGAACAGGTTACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGCGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTCAACCATCAGCGTACCCGCCACCG  >  CP009273/3039555‑3039852

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑