Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,039,703 C→A 100% A900S (GCG→TCG)  gcvP ← glycine decarboxylase, PLP‑dependent, subunit (protein P) of glycine cleavage complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,039,7030CA99.0% 626.5 / ‑1.9 193A900S (GCG→TCG) gcvPglycine decarboxylase, PLP‑dependent, subunit (protein P) of glycine cleavage complex
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base A (94/97);  total (94/99)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 193 total aligned reads displayed.

CAGAACAGGTTACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGCGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTCAACCATCAGCGTA  >  CP009273/3039558‑3039842
                                                                                                                                                 |                                                                                                                                           
    aCAGGTTACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCAc                                                                                                                                       >  2:499996/1‑148 (MQ=255)
     cAGGTTACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCAcc                                                                                                                                      >  2:2773934/1‑148 (MQ=255)
     cAGGTTACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCAcc                                                                                                                                      <  2:2533244/148‑1 (MQ=255)
     cAGGTTACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCAcc                                                                                                                                      >  1:71953/1‑148 (MQ=255)
       ggTTACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAg                                                                                                                                    >  1:3365428/1‑148 (MQ=255)
          tACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCgg                                                                                                                                 >  2:2469500/1‑148 (MQ=255)
           aCGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCggg                                                                                                                                <  2:2023540/148‑1 (MQ=255)
            cGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGtt                                                                                                                                          >  1:961437/1‑137 (MQ=255)
             ggTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGtt                                                                                                                              <  2:3130227/148‑1 (MQ=255)
              gTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTa                                                                                                                             <  1:677020/148‑1 (MQ=255)
                cGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATc                                                                                                                           <  2:46281/148‑1 (MQ=255)
                cGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATc                                                                                                                           >  1:1689343/1‑148 (MQ=255)
                  ccGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGACTACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAAAGGGCGGCGAGTTCACCAGCGGGTGATCtt                                                                                                                         >  2:1168472/1‑148 (MQ=255)
                  ccGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCtt                                                                                                                         >  2:3481856/1‑148 (MQ=255)
                  ccGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCtt                                                                                                                         <  2:170926/148‑1 (MQ=255)
                  ccGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCtt                                                                                                                         <  1:335060/148‑1 (MQ=255)
                   cGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTc                                                                                                                        <  1:1499349/148‑1 (MQ=255)
                    gTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTcc                                                                                                                       <  2:1875665/148‑1 (MQ=255)
                    gTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTcc                                                                                                                       <  2:1667552/148‑1 (MQ=255)
                     tAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCa                                                                                                                      >  1:596255/1‑148 (MQ=255)
                      aaaCATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAg                                                                                                                     >  1:3191143/1‑148 (MQ=255)
                      aaaCATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAg                                                                                                                     >  1:249308/1‑148 (MQ=255)
                         catcatCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCgg                                                                                                                  >  2:2193647/1‑148 (MQ=255)
                         catcatCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCgg                                                                                                                  >  2:1454586/1‑148 (MQ=255)
                            catcCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCa                                                                                                               >  2:3127785/1‑148 (MQ=255)
                            catcCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCa                                                                                                               >  2:120310/1‑148 (MQ=255)
                             atcCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAg                                                                                                              <  1:1342861/148‑1 (MQ=255)
                              tcCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGa                                                                                                             <  1:3059550/148‑1 (MQ=255)
                               ccAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGac                                                                                                            >  1:83728/1‑148 (MQ=255)
                                  gACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACAcc                                                                                                         <  1:1624036/148‑1 (MQ=255)
                                    cGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCgg                                                                                                       >  2:3264370/1‑148 (MQ=255)
                                     gTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGc                                                                                                      <  2:2808416/148‑1 (MQ=255)
                                     gTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGc                                                                                                      <  2:1236095/148‑1 (MQ=255)
                                      tttCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCt                                                                                                     <  1:644665/148‑1 (MQ=255)
                                      tttCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCt                                                                                                     <  1:2958729/148‑1 (MQ=255)
                                      tttCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCt                                                                                                     <  2:2366217/148‑1 (MQ=255)
                                      tttCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCt                                                                                                     <  1:2410100/148‑1 (MQ=255)
                                        tCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCttt                                                                                                   <  1:657909/148‑1 (MQ=255)
                                         cACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTc                                                                                                  >  1:2063813/1‑148 (MQ=255)
                                            tGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCAcc                                                                                               >  1:2940257/1‑148 (MQ=255)
                                             gTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCt                                                                                              >  2:888186/1‑148 (MQ=255)
                                             gTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCt                                                                                              <  1:2539765/148‑1 (MQ=255)
                                               cGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTgg                                                                                            >  2:787713/1‑148 (MQ=255)
                                                  ccAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCa                                                                                         <  2:2093988/148‑1 (MQ=255)
                                                   cAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCaa                                                                                        <  1:3119938/148‑1 (MQ=255)
                                                    aGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAAt                                                                                       >  2:2450905/1‑148 (MQ=255)
                                                      tATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAAttt                                                                                     <  2:2849888/148‑1 (MQ=255)
                                                      tATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAAttt                                                                                     <  1:1666719/148‑1 (MQ=255)
                                                      tATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAAttt                                                                                     >  2:1278292/1‑148 (MQ=255)
                                                       aTTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTc                                                                                    >  2:585311/1‑148 (MQ=255)
                                                            tCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTgcgc                                                                               >  1:1069232/1‑148 (MQ=255)
                                                                ccACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGAt                                                                           <  2:836731/148‑1 (MQ=255)
                                                                 cacaCCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATa                                                                          >  1:1850172/1‑148 (MQ=255)
                                                                  acacCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAg                                                                         >  2:1076697/1‑148 (MQ=255)
                                                                   cacCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGc                                                                        >  2:1158291/1‑148 (MQ=255)
                                                                    acCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGcc                                                                       >  1:865805/1‑148 (MQ=255)
                                                                    acCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGcc                                                                       >  1:3255508/1‑148 (MQ=255)
                                                                      cTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAg                                                                     <  2:2572724/148‑1 (MQ=255)
                                                                            ggAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATcgc                                                               >  2:3510909/1‑148 (MQ=255)
                                                                             gAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATcgcg                                                              <  2:345096/148‑1 (MQ=255)
                                                                               aTACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTc                                                            >  1:2890554/1‑148 (MQ=255)
                                                                                 aCCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGa                                                          <  1:2469500/148‑1 (MQ=255)
                                                                                     cAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATaaa                                                      <  2:2903706/148‑1 (MQ=255)
                                                                                     cAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATaaa                                                      <  1:1860234/148‑1 (MQ=255)
                                                                                      aaCTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAg                                                     <  2:1069220/148‑1 (MQ=255)
                                                                                       aCTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGc                                                    >  1:1193125/1‑148 (MQ=255)
                                                                                       aCTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGc                                                    >  2:2059321/1‑148 (MQ=255)
                                                                                        cTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCg                                                   <  1:186348/148‑1 (MQ=255)
                                                                                        cTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCg                                                   <  1:186945/148‑1 (MQ=255)
                                                                                        cTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCg                                                   <  1:802017/148‑1 (MQ=255)
                                                                                        cTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCg                                                   >  2:2453606/1‑148 (MQ=255)
                                                                                         ttCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGa                                                  >  2:1426428/1‑148 (MQ=255)
                                                                                            aCGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATcc                                               <  1:499996/148‑1 (MQ=255)
                                                                                            aCGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATcc                                               <  1:572968/148‑1 (MQ=255)
                                                                                              ggCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAg                                             <  1:2916465/148‑1 (MQ=255)
                                                                                                   tACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCTTCGATAAAGCGATCCAGTTCCa                                        >  1:1027552/1‑148 (MQ=255)
                                                                                                   tACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCa                                        <  1:2022526/148‑1 (MQ=255)
                                                                                                    aCGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCAc                                       >  1:2702138/1‑148 (MQ=255)
                                                                                                          gcgcCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCt                                 <  1:3507688/148‑1 (MQ=255)
                                                                                                          gcgcCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCt                                 <  1:2123203/148‑1 (MQ=255)
                                                                                                            gcCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCttt                               <  1:3169828/148‑1 (MQ=255)
                                                                                                            gcCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCttt                               <  1:1493662/148‑1 (MQ=255)
                                                                                                                   cGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTc                        >  2:2331655/1‑148 (MQ=255)
                                                                                                                   cGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTc                        >  2:1732969/1‑148 (MQ=255)
                                                                                                                    ggCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCa                       <  1:2153506/148‑1 (MQ=255)
                                                                                                                        aCCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCg                   >  2:2568776/1‑148 (MQ=255)
                                                                                                                         ccAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCgg                  <  2:71953/148‑1 (MQ=255)
                                                                                                                            gTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTc               >  1:438429/1‑148 (MQ=255)
                                                                                                                             ttCGCTCTGAATGTGCGGCGCGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTCa              <  2:2839378/148‑1 (MQ=255)
                                                                                                                             ttCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTCa              >  1:3403831/1‑148 (MQ=255)
                                                                                                                             ttCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTCa              <  1:1063986/148‑1 (MQ=255)
                                                                                                                               cGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTCAAc            <  1:41832/148‑1 (MQ=255)
                                                                                                                                gCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCACTTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTCAAcc           <  1:1816097/148‑1 (MQ=255)
                                                                                                                                 ctctGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTCAACCa          >  2:3469974/1‑148 (MQ=255)
                                                                                                                                  tctGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTCAACCAt         <  2:1946494/148‑1 (MQ=255)
                                                                                                                                  tctGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTCAACCAt         <  1:1454586/148‑1 (MQ=255)
                                                                                                                                   ctGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTCAACCATc        <  2:2064460/148‑1 (MQ=255)
                                                                                                                                      aaTGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCTACTTTGCTTTCAGATTCAGTCGGTTCAACCATCAGc     <  2:2043865/148‑1 (MQ=255)
                                                                                                                                       aTGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGACAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTCAACCATCAGCg    <  1:3341787/148‑1 (MQ=255)
                                                                                                                                         gtgCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTCAACCATCAGCGTa  <  2:3365428/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                           
CAGAACAGGTTACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGCGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTCAACCATCAGCGTA  >  CP009273/3039558‑3039842

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGAGCAGAACAGGTTACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGCGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTCAACCATCAGCGTACCCGCCACC  >  CP009273/3039554‑3039851
                                                                                                                                                     |                                                                                                                                                    
GGAGCAGAACAGGTTACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGA                                                                                                                                                      >  SRR19973065.2019808/1‑150 (MQ=60)
 GAGCAGAACAGGTTACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAG                                                                                                                                                     <  SRR19973065.2776083/150‑1 (MQ=60)
 GAGCAGAACAGGTTACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAG                                                                                                                                                     <  SRR19973065.2566580/150‑1 (MQ=60)
  AGCAGAACAGGTTACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGT                                                                                                                                                    >  SRR19973065.1665309/1‑150 (MQ=60)
  AGCAGAACAGGTTACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGT                                                                                                                                                    >  SRR19973065.842184/1‑150 (MQ=60)
   GCAGAACAGGTTACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTT                                                                                                                                                   <  SRR19973065.3312646/150‑1 (MQ=60)
       AACAGGTTACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACC                                                                                                                                               >  SRR19973065.1861733/1‑150 (MQ=60)
       AACAGGTTACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACC                                                                                                                                               >  SRR19973065.72702/1‑150 (MQ=60)
         CAGGTTACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAG                                                                                                                                             >  SRR19973065.3386006/1‑150 (MQ=60)
              TACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCAagatcggaa                                                                                                                                        >  SRR19973065.967814/1‑141 (MQ=60)
                  GTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATC                                                                                                                                    <  SRR19973065.681533/150‑1 (MQ=60)
                  GTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATC                                                                                                                                    >  SRR19973065.1700623/1‑150 (MQ=60)
                  GTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATC                                                                                                                                    <  SRR19973065.2454121/150‑1 (MQ=60)
                  GTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGGTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATC                                                                                                                                    >  SRR19973065.1699263/1‑150 (MQ=60)
                   TCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCT                                                                                                                                   <  SRR19973065.2859854/150‑1 (MQ=60)
                      gccttAACATAATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCC                                                                                                                                <  SRR19973065.3225421/145‑1 (MQ=60)
                      CCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCC                                                                                                                                <  SRR19973065.337405/150‑1 (MQ=60)
                      CCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCC                                                                                                                                <  SRR19973065.336543/150‑1 (MQ=60)
                       CGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCA                                                                                                                               <  SRR19973065.1509015/150‑1 (MQ=60)
                       CGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCA                                                                                                                               >  SRR19973065.599953/1‑150 (MQ=60)
                        GTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAG                                                                                                                              >  SRR19973065.251061/1‑150 (MQ=60)
                        GTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAG                                                                                                                              >  SRR19973065.3525681/1‑150 (MQ=60)
                        GTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAG                                                                                                                              <  SRR19973065.3530699/150‑1 (MQ=60)
                        GTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAG                                                                                                                              >  SRR19973065.3210392/1‑150 (MQ=60)
                        GTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAG                                                                                                                              >  SRR19973065.3210368/1‑150 (MQ=60)
                          AAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACATCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCG                                                                                                                            >  SRR19973065.2673816/1‑150 (MQ=60)
                             CATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCC                                                                                                                         >  SRR19973065.1493379/1‑150 (MQ=60)
                              ATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCA                                                                                                                        <  SRR19973065.893903/150‑1 (MQ=60)
                              ATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCA                                                                                                                        >  SRR19973065.2081802/1‑150 (MQ=60)
                                 ATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGAC                                                                                                                     <  SRR19973065.1351745/150‑1 (MQ=60)
                                 ATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGAC                                                                                                                     >  SRR19973065.84548/1‑150 (MQ=60)
                                  TCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACA                                                                                                                    <  SRR19973065.3078254/150‑1 (MQ=60)
                                  TCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACA                                                                                                                    <  SRR19973065.3359021/150‑1 (MQ=60)
                                    CAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACANC                                                                                                                  <  SRR19973065.1972639/150‑1 (MQ=60)
                                    CAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACC                                                                                                                  <  SRR19973065.187552/150‑1 (MQ=60)
                                      GACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGG                                                                                                                <  SRR19973065.1634923/150‑1 (MQ=60)
                                       ACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGC                                                                                                               >  SRR19973065.1730600/1‑150 (MQ=60)
                                          TTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTT                                                                                                            <  SRR19973065.2976796/150‑1 (MQ=60)
                                          TTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTT                                                                                                            <  SRR19973065.2960851/150‑1 (MQ=60)
                                          TTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTT                                                                                                            <  SRR19973065.2425275/150‑1 (MQ=60)
                                          TTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTT                                                                                                            <  SRR19973065.648918/150‑1 (MQ=60)
                                          TTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTT                                                                                                            <  SRR19973065.648899/150‑1 (MQ=60)
                                           TTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTC                                                                                                           >  SRR19973065.712646/1‑150 (MQ=60)
                                           TTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTC                                                                                                           >  SRR19973065.2076973/1‑150 (MQ=60)
                                           TTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTC                                                                                                           >  SRR19973065.3394561/1‑150 (MQ=60)
                                            TCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCA                                                                                                          <  SRR19973065.662253/150‑1 (MQ=60)
                                              ACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACC                                                                                                        >  SRR19973065.2958098/1‑150 (MQ=60)
                                              ACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACC                                                                                                        >  SRR19973065.2958040/1‑150 (MQ=60)
                                                 GTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGG                                                                                                     <  SRR19973065.2555683/150‑1 (MQ=60)
                                                   CGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTC                                                                                                   <  SRR19973065.3135268/150‑1 (MQ=60)
                                                     GCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAA                                                                                                 >  SRR19973065.1269863/1‑150 (MQ=60)
                                                       CAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATT                                                                                               <  SRR19973065.3138947/150‑1 (MQ=60)
                                                          TATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCT                                                                                            <  SRR19973065.1677873/150‑1 (MQ=60)
                                                          TATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCT                                                                                            >  SRR19973065.3378062/1‑150 (MQ=60)
                                                          TATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCT                                                                                            <  SRR19973065.1162185/150‑1 (MQ=60)
                                                              TGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGC                                                                                        >  SRR19973065.1076300/1‑150 (MQ=60)
                                                              TGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGC                                                                                        >  SRR19973065.1076312/1‑150 (MQ=60)
                                                                 CTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGA                                                                                     >  SRR19973065.738152/1‑150 (MQ=60)
                                                                   GCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATA                                                                                   >  SRR19973065.1862364/1‑150 (MQ=60)
                                                                      ACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCC                                                                                >  SRR19973065.3275194/1‑150 (MQ=60)
                                                                      ACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCC                                                                                >  SRR19973065.871366/1‑150 (MQ=60)
                                                                           TGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCAT                                                                           <  SRR19973065.2530149/150‑1 (MQ=60)
                                                                            GCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATC                                                                          <  SRR19973065.452435/150‑1 (MQ=60)
                                                                                GGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGT                                                                      >  SRR19973065.43389/1‑150 (MQ=60)
                                                                                 GAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTC                                                                     >  SRR19973065.2908217/1‑150 (MQ=60)
                                                                                 GAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTC                                                                     >  SRR19973065.2908322/1‑150 (MQ=60)
                                                                                     ACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATA                                                                 <  SRR19973065.2485051/150‑1 (MQ=60)
                                                                                         CAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGC                                                             >  SRR19973065.1200704/1‑150 (MQ=60)
                                                                                         CAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGC                                                             <  SRR19973065.1872472/150‑1 (MQ=60)
                                                                                            CTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGAT                                                          <  SRR19973065.187810/150‑1 (MQ=60)
                                                                                            CTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGAT                                                          <  SRR19973065.807319/150‑1 (MQ=60)
                                                                                            CTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGAT                                                          <  SRR19973065.188412/150‑1 (MQ=60)
                                                                                              TCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCC                                                        >  SRR19973065.2044449/1‑150 (MQ=60)
                                                                                               CACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCA                                                       <  SRR19973065.869806/150‑1 (MQ=60)
                                                                                                ACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAG                                                      <  SRR19973065.576580/150‑1 (MQ=60)
                                                                                                ACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAG                                                      <  SRR19973065.576071/150‑1 (MQ=60)
                                                                                                ACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAG                                                      <  SRR19973065.503349/150‑1 (MQ=60)
                                                                                                 CGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGT                                                     <  SRR19973065.676231/150‑1 (MQ=60)
                                                                                                  GGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTT                                                    <  SRR19973065.2934206/150‑1 (MQ=60)
                                                                                                     TGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCTTCGATAAAGCGATCCAGTTCCA                                                 >  SRR19973065.1034353/1‑150 (MQ=60)
                                                                                                      GTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCAC                                                >  SRR19973065.2718936/1‑150 (MQ=60)
                                                                                                       TACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACT                                               <  SRR19973065.2035534/150‑1 (MQ=60)
                                                                                                              GCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTT                                        <  SRR19973065.3528973/150‑1 (MQ=60)
                                                                                                              GCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTT                                        <  SRR19973065.2136875/150‑1 (MQ=60)
                                                                                                                GCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCA                                      <  SRR19973065.1503767/150‑1 (MQ=60)
                                                                                                                GCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCA                                      <  SRR19973065.1503255/150‑1 (MQ=60)
                                                                                                                GCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCA                                      <  SRR19973065.3189013/150‑1 (MQ=60)
                                                                                                                        GGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGT                              <  SRR19973065.2167345/150‑1 (MQ=60)
                                                                                                                        GGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGT                              <  SRR19973065.2167356/150‑1 (MQ=60)
                                                                                                                        GGCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGT                              <  SRR19973065.2497580/150‑1 (MQ=60)
                                                                                                                         GCGACCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTC                             <  SRR19973065.3175454/150‑1 (MQ=60)
                                                                                                                             CCAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCATTTCCACTTTGCTTTCAGATTCAGTCGGTT                         >  SRR19973065.1864383/1‑150 (MQ=60)
                                                                                                                              CAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTC                        >  SRR19973065.441467/1‑150 (MQ=60)
                                                                                                                              CAGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTC                        >  SRR19973065.2229637/1‑150 (MQ=60)
                                                                                                                               AGTTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTCA                       >  SRR19973065.3424631/1‑150 (MQ=60)
                                                                                                                                 TTCGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTCAAC                     <  SRR19973065.1071038/150‑1 (MQ=60)
                                                                                                                                   CGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTCAACCA                   >  SRR19973065.631393/1‑150 (MQ=60)
                                                                                                                                   CGCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTCAACCA                   <  SRR19973065.42342/150‑1 (MQ=60)
                                                                                                                                    GCTCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCACTTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTCAACCAT                  <  SRR19973065.1828057/150‑1 (MQ=60)
                                                                                                                                      TCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTCAACCATCA                <  SRR19973065.1463957/150‑1 (MQ=60)
                                                                                                                                      TCTGAATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTCAACCATCA                <  SRR19973065.1463914/150‑1 (MQ=60)
                                                                                                                                           ATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGACAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTCAACCATCAGCGTA           <  SRR19973065.1613633/150‑1 (MQ=60)
                                                                                                                                           ATGTGCGGCGAGTTCACCAGCGGGTTATCTTCCAGCGGACAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTCAACCATCAGCGTA           <  SRR19973065.3362205/150‑1 (MQ=60)
                                                                                                                                                    GAGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTCAACCATCAGCGTACCCGCCACC  <  SRR19973065.215496/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
GGAGCAGAACAGGTTACGGTCGCCGTAAACATCATCCAGACGTTTCACTGTCGGCCAGTATTTGTCTGCCACACCTGCCGGGAATACCGCAACTTCACGGCTGTACGGATGCGCCCACTCGGCGACCAGTTCGCTCTGAATGTGCGGCGCGTTCACCAGCGGGTTATCTTCCAGCGGCCAGACACCGGCTTTCACCTGGTCAATTTCTGCGCGGATAGCCAGCATCGCGTCGATAAAGCGATCCAGTTCCACTTTGCTTTCAGATTCAGTCGGTTCAACCATCAGCGTACCCGCCACC  >  CP009273/3039554‑3039851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑