Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,064,914 G→T 100% I356I (ATC→ATA)  pgk ← phosphoglycerate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,064,9140GT99.7% 817.1 / ‑5.9 253I356I (ATC→ATA) pgkphosphoglycerate kinase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (105/147);  total (106/147)
Fisher's exact test for biased strand distribution p-value = 4.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 257 total aligned reads displayed.

CTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGAGATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGAC  >  CP009273/3064769‑3065059
                                                                                                                                                 |                                                                                                                                                 
ctctAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGatat                                                                                                                                                 <  2:1479653/148‑1 (MQ=255)
ctctAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGatat                                                                                                                                                 <  1:3150349/148‑1 (MQ=255)
 tctAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATAtt                                                                                                                                                <  2:3118989/148‑1 (MQ=255)
  ctAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATAttt                                                                                                                                               <  2:2717110/148‑1 (MQ=255)
  ctAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATAttt                                                                                                                                               <  2:2493008/148‑1 (MQ=255)
      aaGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTc                                                                                                                                           <  2:385653/148‑1 (MQ=255)
      aaGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTc                                                                                                                                           >  1:1589946/1‑148 (MQ=255)
       aGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCa                                                                                                                                          >  2:1301207/1‑148 (MQ=255)
       aGCGCGCTGAAACAAGGGCAGGTTTCCATGCCCTGTGATTTTTTACTTCTTAGCGCGCTCATCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATCTTTTGTCa                                                                                                                                          >  2:1955219/1‑148 (MQ=255)
        gcgcgcTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAg                                                                                                                                         >  1:3045630/1‑148 (MQ=255)
         cgcgcTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGc                                                                                                                                        >  1:1675256/1‑148 (MQ=255)
         cgcgcTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGc                                                                                                                                        >  1:2809597/1‑148 (MQ=255)
          gcgcTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCa                                                                                                                                       <  2:1382197/148‑1 (MQ=255)
           cgcTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCaa                                                                                                                                      >  2:1238134/1‑148 (MQ=255)
             cTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATg                                                                                                                                    >  2:1296662/1‑148 (MQ=255)
             cTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATg                                                                                                                                    <  1:595421/148‑1 (MQ=255)
              tGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGc                                                                                                                                   <  1:2154513/148‑1 (MQ=255)
                  aCAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGaa                                                                                                                               >  2:2240529/1‑148 (MQ=255)
                   cAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAAc                                                                                                                              <  1:863515/148‑1 (MQ=255)
                   cAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAAc                                                                                                                              <  1:598365/148‑1 (MQ=255)
                     aGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAg                                                                                                                            <  2:2966772/148‑1 (MQ=255)
                       ggCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGt                                                                                                                          <  1:1639290/148‑1 (MQ=255)
                              ttCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATtgc                                                                                                                   <  1:1924150/148‑1 (MQ=255)
                                 cctgccctgTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGACCAGGTCGATtgctgc                                                                                                                >  2:3037408/1‑148 (MQ=255)
                                       ctgTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAgt                                                                                                          <  2:101092/148‑1 (MQ=255)
                                       ctgTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAgt                                                                                                          >  2:1752587/1‑148 (MQ=255)
                                       ctgTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAgt                                                                                                          >  2:1132669/1‑148 (MQ=255)
                                       ctgTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAgt                                                                                                          >  2:1585950/1‑148 (MQ=255)
                                       ctgTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAgt                                                                                                          >  1:1978181/1‑148 (MQ=255)
                                        tgtgATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAgtg                                                                                                         >  1:1643521/1‑148 (MQ=255)
                                         gtgATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAgtgt                                                                                                        <  1:2101596/148‑1 (MQ=255)
                                                    cttcttAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCa                                                                                             >  1:2429974/1‑148 (MQ=255)
                                                     ttcttAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAg                                                                                            >  1:1655325/1‑148 (MQ=255)
                                                       cttAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTAGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCg                                                                                          <  2:1985886/148‑1 (MQ=255)
                                                         tAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGAt                                                                                        >  2:2074798/1‑148 (MQ=255)
                                                           gcgcgcTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATgg                                                                                      >  1:2695960/1‑148 (MQ=255)
                                                            cgcgcTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGga                                                                                     <  2:2416904/148‑1 (MQ=255)
                                                            cgcgcTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGga                                                                                     >  2:3244248/1‑148 (MQ=255)
                                                              cgcTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGgaga                                                                                   <  2:2603924/148‑1 (MQ=255)
                                                              cgcTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGgaga                                                                                   <  1:640233/148‑1 (MQ=255)
                                                               gcTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGGAGGATCGTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGaa                                                                                  >  1:3215465/1‑148 (MQ=255)
                                                                 tctTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACg                                                                                <  2:2356335/148‑1 (MQ=255)
                                                                 tctTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACg                                                                                <  1:1465729/148‑1 (MQ=255)
                                                                  ctTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGc                                                                               <  1:2052386/148‑1 (MQ=255)
                                                                  ctTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGc                                                                               <  2:3195396/148‑1 (MQ=255)
                                                                  ctTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGc                                                                               <  2:1150403/148‑1 (MQ=255)
                                                                   ttCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCTCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTAGCCGCCGCCAGCTATGGAGAACGCt                                                                              <  1:1065113/148‑1 (MQ=255)
                                                                   ttCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCt                                                                              >  2:2197571/1‑148 (MQ=255)
                                                                   ttCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCt                                                                              <  2:537573/148‑1 (MQ=255)
                                                                            cGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACATGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCt                                                                     >  1:2366535/1‑148 (MQ=255)
                                                                            cGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCt                                                                     >  1:536974/1‑148 (MQ=255)
                                                                             gCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTg                                                                    <  1:404395/148‑1 (MQ=255)
                                                                                   gcaggcagTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAg                                                              <  2:1436993/148‑1 (MQ=255)
                                                                                      ggcagTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGt                                                           <  1:2260127/148‑1 (MQ=255)
                                                                                       gcagTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGtt                                                          >  2:2650463/1‑148 (MQ=255)
                                                                                       gcagTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGtt                                                          <  2:2852933/148‑1 (MQ=255)
                                                                                       gcagTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGAGGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGtt                                                          >  2:2263695/1‑148 (MQ=255)
                                                                                        cagTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTa                                                         <  1:1427555/148‑1 (MQ=255)
                                                                                          gTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGc                                                       >  1:5726/1‑148 (MQ=255)
                                                                                           tACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTTGCTGTCTGCGATAGCGTTAGcc                                                      <  2:2366397/148‑1 (MQ=255)
                                                                                            aCTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCa                                                     <  2:1675256/148‑1 (MQ=255)
                                                                                              tttACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACg                                                   >  2:1865427/1‑148 (MQ=255)
                                                                                                tACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGAt                                                 >  1:1859483/1‑148 (MQ=255)
                                                                                                tACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGAt                                                 <  1:3262919/148‑1 (MQ=255)
                                                                                                  ccTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGAttt                                               <  1:1151877/148‑1 (MQ=255)
                                                                                                  ccTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGAttt                                               <  2:1368966/148‑1 (MQ=255)
                                                                                                  ccTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGAttt                                               >  1:1815477/1‑148 (MQ=255)
                                                                                                  ccTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGAttt                                               >  1:1281424/1‑148 (MQ=255)
                                                                                                   cTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTc                                              >  2:3166687/1‑148 (MQ=255)
                                                                                                     tCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAg                                            <  2:3095317/148‑1 (MQ=255)
                                                                                                           aaTTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCtt                                      <  2:918646/148‑1 (MQ=255)
                                                                                                           aaTTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCtt                                      <  2:2336424/148‑1 (MQ=255)
                                                                                                           aaTTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCACTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCtt                                      >  1:1948414/1‑148 (MQ=255)
                                                                                                             ttCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTg                                    <  1:2246879/148‑1 (MQ=255)
                                                                                                             ttCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTg                                    >  1:3065962/1‑148 (MQ=255)
                                                                                                             ttCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTg                                    <  2:320173/148‑1 (MQ=255)
                                                                                                             ttCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTg                                    >  2:2079528/1‑148 (MQ=255)
                                                                                                              tCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGc                                   <  2:752701/148‑1 (MQ=255)
                                                                                                                 aGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGa                                <  1:3244248/148‑1 (MQ=255)
                                                                                                                 aGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGa                                <  2:1368358/148‑1 (MQ=255)
                                                                                                                    aaTGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGt                             <  2:3268219/148‑1 (MQ=255)
                                                                                                                    aaTGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGt                             >  2:1569238/1‑148 (MQ=255)
                                                                                                                     aTGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGtt                            >  1:318892/1‑148 (MQ=255)
                                                                                                                           cgccgccAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGaa                      <  2:1774310/148‑1 (MQ=255)
                                                                                                                            gccgccAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAAt                     <  1:61513/148‑1 (MQ=255)
                                                                                                                               gccAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCg                  <  1:1765653/148‑1 (MQ=255)
                                                                                                                                 cAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTGGaa                <  1:1872703/148‑1 (MQ=255)
                                                                                                                                 cAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTGGaa                <  1:1361867/148‑1 (MQ=255)
                                                                                                                                  aGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAac               <  2:1423938/148‑1 (MQ=255)
                                                                                                                                   gTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAaca              <  1:2989351/148‑1 (MQ=255)
                                                                                                                                    tGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAacac             >  1:1228840/1‑148 (MQ=255)
                                                                                                                                       agaTGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGcc          >  2:3222169/1‑148 (MQ=255)
                                                                                                                                        gaTGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCa         <  2:2763735/148‑1 (MQ=255)
                                                                                                                                           gTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAAcc      >  2:574447/1‑148 (MQ=255)
                                                                                                                                           gTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAAcc      >  2:668285/1‑148 (MQ=255)
                                                                                                                                             aGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCgg    >  1:518210/1‑148 (MQ=255)
                                                                                                                                              ggATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGa   <  1:1337097/148‑1 (MQ=255)
                                                                                                                                              ggATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGa   >  1:453244/1‑148 (MQ=255)
                                                                                                                                              ggATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGa   <  1:2929542/148‑1 (MQ=255)
                                                                                                                                              ggATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGa   <  1:2650463/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
CTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGAGATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGAC  >  CP009273/3064769‑3065059

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGAGATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGT  >  CP009273/3064769‑3065062
                                                                                                                                                 |                                                                                                                                                    
CTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTT                                                                                                                                                  <  SRR19973070.3170708/150‑1 (MQ=60)
  CTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTG                                                                                                                                                <  SRR19973070.1391037/150‑1 (MQ=60)
    AAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTC                                                                                                                                              <  SRR19973070.1609657/150‑1 (MQ=60)
    AAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTC                                                                                                                                              >  SRR19973070.1601069/1‑150 (MQ=60)
    AAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTC                                                                                                                                              <  SRR19973070.3259763/150‑1 (MQ=60)
      AAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAG                                                                                                                                            >  SRR19973070.3064995/1‑150 (MQ=60)
       AGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGC                                                                                                                                           >  SRR19973070.1686727/1‑150 (MQ=60)
       AGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGC                                                                                                                                           >  SRR19973070.2827762/1‑150 (MQ=60)
       AGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGC                                                                                                                                           >  SRR19973070.2827892/1‑150 (MQ=60)
             CTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCC                                                                                                                                     <  SRR19973070.599843/150‑1 (MQ=60)
             CTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCC                                                                                                                                     <  SRR19973070.599556/150‑1 (MQ=60)
              TGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCG                                                                                                                                    <  SRR19973070.2168847/150‑1 (MQ=60)
                  ACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACA                                                                                                                                <  SRR19973070.3071569/150‑1 (MQ=60)
                  ACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACA                                                                                                                                <  SRR19973070.596242/150‑1 (MQ=60)
                   CAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAG                                                                                                                               <  SRR19973070.602528/150‑1 (MQ=60)
                   CAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAG                                                                                                                               <  SRR19973070.602575/150‑1 (MQ=60)
                   CAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAG                                                                                                                               <  SRR19973070.869528/150‑1 (MQ=60)
                   CAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAG                                                                                                                               <  SRR19973070.1752255/150‑1 (MQ=60)
                    AAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGG                                                                                                                              <  SRR19973070.2136197/150‑1 (MQ=60)
                       GGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCG                                                                                                                           <  SRR19973070.1650638/150‑1 (MQ=60)
                           GGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTG                                                                                                                       <  SRR19973070.584383/150‑1 (MQ=60)
                             TTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCT                                                                                                                     <  SRR19973070.3259474/150‑1 (MQ=60)
                             TTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCT                                                                                                                     <  SRR19973070.174832/150‑1 (MQ=60)
                              TTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTG                                                                                                                    >  SRR19973070.3289243/1‑150 (MQ=60)
                              TTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTG                                                                                                                    <  SRR19973070.1937119/150‑1 (MQ=60)
                              TTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTG                                                                                                                    <  SRR19973070.1937199/150‑1 (MQ=60)
                                  CTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAG                                                                                                                >  SRR19973070.2345042/1‑150 (MQ=60)
                                  CTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAG                                                                                                                >  SRR19973070.2878969/1‑150 (MQ=60)
                                     CCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGT                                                                                                             >  SRR19973070.1991554/1‑150 (MQ=60)
                                      CCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTG                                                                                                            >  SRR19973070.1654887/1‑150 (MQ=60)
                                      CCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTG                                                                                                            >  SRR19973070.1654880/1‑150 (MQ=60)
                                        TGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTC                                                                                                          <  SRR19973070.1333657/150‑1 (MQ=60)
                                         GTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCG                                                                                                         <  SRR19973070.2115701/150‑1 (MQ=60)
                                                  TACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCA                                                                                                >  SRR19973070.2445927/1‑150 (MQ=60)
                                                   ACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAG                                                                                               >  SRR19973070.1666734/1‑150 (MQ=60)
                                                   ACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAG                                                                                               >  SRR19973070.1666707/1‑150 (MQ=60)
                                                         TAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGAGATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGG                                                                                         >  SRR19973070.2713417/1‑150 (MQ=60)
                                                           GCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAG                                                                                       <  SRR19973070.693552/150‑1 (MQ=60)
                                                             GCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAA                                                                                     >  SRR19973070.3031072/1‑150 (MQ=60)
                                                             GCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGGAGGATCGTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAA                                                                                     >  SRR19973070.3236257/1‑150 (MQ=60)
                                                              CGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAAC                                                                                    <  SRR19973070.644796/150‑1 (MQ=60)
                                                               GCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACG                                                                                   <  SRR19973070.2776207/150‑1 (MQ=60)
                                                                 TCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCT                                                                                 <  SRR19973070.1475970/150‑1 (MQ=60)
                                                                 TCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCT                                                                                 <  SRR19973070.1475978/150‑1 (MQ=60)
                                                                  CTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTT                                                                                <  SRR19973070.2066262/150‑1 (MQ=60)
                                                                   TTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCTCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTAGCCGCCGCCAGCTATGGAGAACGCTTC                                                                               <  SRR19973070.1072574/150‑1 (MQ=60)
                                                                     CGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGC                                                                             >  SRR19973070.3118699/1‑150 (MQ=60)
                                                                       AGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTG                                                                           <  SRR19973070.2146922/150‑1 (MQ=60)
                                                                          ATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACATGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCT                                                                        >  SRR19973070.2383570/1‑150 (MQ=60)
                                                                          ATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACATGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCT                                                                        >  SRR19973070.2382188/1‑150 (MQ=60)
                                                                          ATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCT                                                                        <  SRR19973070.2811015/150‑1 (MQ=60)
                                                                          ATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCT                                                                        >  SRR19973070.540575/1‑150 (MQ=60)
                                                                             GCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCG                                                                     >  SRR19973070.1850880/1‑150 (MQ=60)
                                                                             GCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCG                                                                     >  SRR19973070.168647/1‑150 (MQ=60)
                                                                             GCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCG                                                                     <  SRR19973070.407366/150‑1 (MQ=60)
                                                                              CTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGA                                                                    >  SRR19973070.638416/1‑150 (MQ=60)
                                                                               TACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGAT                                                                   <  SRR19973070.1652029/150‑1 (MQ=60)
                                                                                ACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATA                                                                  >  SRR19973070.3219517/1‑150 (MQ=60)
                                                                                ACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATA                                                                  >  SRR19973070.3220007/1‑150 (MQ=60)
                                                                                 CTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAG                                                                 <  SRR19973070.909006/150‑1 (MQ=60)
                                                                                  TGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGC                                                                >  SRR19973070.455867/1‑150 (MQ=60)
                                                                                   GCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCG                                                               <  SRR19973070.1649578/150‑1 (MQ=60)
                                                                                      GGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTA                                                            <  SRR19973070.2275028/150‑1 (MQ=60)
                                                                                      GGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTA                                                            <  SRR19973070.2275122/150‑1 (MQ=60)
                                                                                        CAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGC                                                          >  SRR19973070.5814/1‑150 (MQ=60)
                                                                                        CAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGC                                                          <  SRR19973070.1369372/150‑1 (MQ=60)
                                                                                        CAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGC                                                          <  SRR19973070.1437396/150‑1 (MQ=60)
                                                                                        CAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGC                                                          <  SRR19973070.1437403/150‑1 (MQ=60)
                                                                                          GTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCA                                                        <  SRR19973070.1887650/150‑1 (MQ=60)
                                                                                             CTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGA                                                     >  SRR19973070.508841/1‑150 (MQ=60)
                                                                                              TTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGAT                                                    >  SRR19973070.1872206/1‑150 (MQ=60)
                                                                                              TTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGAT                                                    >  SRR19973070.1872228/1‑150 (MQ=60)
                                                                                                TACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTT                                                  >  SRR19973070.1827986/1‑150 (MQ=60)
                                                                                                TACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTT                                                  >  SRR19973070.1827989/1‑150 (MQ=60)
                                                                                                TACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTT                                                  <  SRR19973070.2172903/150‑1 (MQ=60)
                                                                                                TACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTT                                                  <  SRR19973070.3283920/150‑1 (MQ=60)
                                                                                                TACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTT                                                  >  SRR19973070.1290322/1‑150 (MQ=60)
                                                                                                TACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTT                                                  >  SRR19973070.1290374/1‑150 (MQ=60)
                                                                                                  CCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCA                                                <  SRR19973070.1159913/150‑1 (MQ=60)
                                                                                                  CCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCA                                                <  SRR19973070.1160262/150‑1 (MQ=60)
                                                                                                  CCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCA                                                <  SRR19973070.1160281/150‑1 (MQ=60)
                                                                                                    TTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGT                                              >  SRR19973070.395181/1‑150 (MQ=60)
                                                                                                     TCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTA                                             >  SRR19973070.2071951/1‑150 (MQ=60)
                                                                                                         CGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTT                                         >  SRR19973070.1961537/1‑150 (MQ=60)
                                                                                                         CGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCACTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTT                                         >  SRR19973070.1961497/1‑150 (MQ=60)
                                                                                                          GAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTT                                        >  SRR19973070.809285/1‑150 (MQ=60)
                                                                                                           AATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTG                                       >  SRR19973070.1993060/1‑150 (MQ=60)
                                                                                                           AATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTG                                       >  SRR19973070.3085610/1‑150 (MQ=60)
                                                                                                           AATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTG                                       >  SRR19973070.2002825/1‑150 (MQ=60)
                                                                                                             TTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCG                                     <  SRR19973070.2261737/150‑1 (MQ=60)
                                                                                                                 AGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCGGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAG                                 >  SRR19973070.605984/1‑150 (MQ=60)
                                                                                                                 AGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCGGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAG                                 >  SRR19973070.3156560/1‑150 (MQ=60)
                                                                                                                 AGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCGGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAG                                 >  SRR19973070.2554692/1‑150 (MQ=60)
                                                                                                                 AGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAG                                 <  SRR19973070.3265886/150‑1 (MQ=60)
                                                                                                                 AGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAG                                 <  SRR19973070.3265161/150‑1 (MQ=60)
                                                                                                                   GAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTT                               >  SRR19973070.321202/1‑150 (MQ=60)
                                                                                                                   GAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTT                               <  SRR19973070.228253/150‑1 (MQ=60)
                                                                                                                   GAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTT                               >  SRR19973070.321201/1‑150 (MQ=60)
                                                                                                                    AATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTC                              <  SRR19973070.2992884/150‑1 (MQ=60)
                                                                                                                          CCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAAT                        <  SRR19973070.1459116/150‑1 (MQ=60)
                                                                                                                           CGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATT                       >  SRR19973070.1543033/1‑150 (MQ=60)
                                                                                                                            GCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTC                      <  SRR19973070.62208/150‑1 (MQ=60)
                                                                                                                               GCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAA                   <  SRR19973070.1777865/150‑1 (MQ=60)
                                                                                                                                 CAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTGGAACA                 <  SRR19973070.1885474/150‑1 (MQ=60)
                                                                                                                                 CAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTGGAACA                 <  SRR19973070.1371155/150‑1 (MQ=60)
                                                                                                                                 CAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACA                 <  SRR19973070.1345711/150‑1 (MQ=60)
                                                                                                                                 CAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACA                 <  SRR19973070.1869292/150‑1 (MQ=60)
                                                                                                                                  AGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACAC                >  SRR19973070.1237446/1‑150 (MQ=60)
                                                                                                                                   GTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACG               <  SRR19973070.3009211/150‑1 (MQ=60)
                                                                                                                                   GTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACG               <  SRR19973070.3008505/150‑1 (MQ=60)
                                                                                                                                       AGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAA           >  SRR19973070.2685751/1‑150 (MQ=60)
                                                                                                                                        GATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATGGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCTGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAAC          <  SRR19973070.195959/150‑1 (MQ=60)
                                                                                                                                         ATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGTCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACC         <  SRR19973070.2212067/150‑1 (MQ=60)
                                                                                                                                           GTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGG       >  SRR19973070.1878315/1‑150 (MQ=60)
                                                                                                                                           GTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGG       >  SRR19973070.521975/1‑150 (MQ=60)
                                                                                                                                           GTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGG       >  SRR19973070.521749/1‑150 (MQ=60)
                                                                                                                                           GTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGG       >  SRR19973070.462820/1‑150 (MQ=60)
                                                                                                                                            TAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGA      >  SRR19973070.572434/1‑150 (MQ=60)
                                                                                                                                            TAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGA      >  SRR19973070.572149/1‑150 (MQ=60)
                                                                                                                                            TAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGA      >  SRR19973070.1108878/1‑150 (MQ=60)
                                                                                                                                            TAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGA      >  SRR19973070.456505/1‑150 (MQ=60)
                                                                                                                                            TAGGATATTTGGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGA      <  SRR19973070.2548393/150‑1 (MQ=60)
                                                                                                                                             AGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGAC     >  SRR19973070.2521983/1‑150 (MQ=60)
                                                                                                                                             AGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGAC     >  SRR19973070.2524035/1‑150 (MQ=60)
                                                                                                                                              GGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACC    <  SRR19973070.1346295/150‑1 (MQ=60)
                                                                                                                                              GGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACC    <  SRR19973070.2667709/150‑1 (MQ=60)
                                                                                                                                              GGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACC    <  SRR19973070.2948445/150‑1 (MQ=60)
                                                                                                                                               GATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCG   >  SRR19973070.1105574/1‑150 (MQ=60)
                                                                                                                                                ATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGT  >  SRR19973070.486180/1‑150 (MQ=60)
                                                                                                                                                 |                                                                                                                                                    
CTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGAGATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGT  >  CP009273/3064769‑3065062

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑