Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,064,914 G→T 100% I356I (ATC→ATA)  pgk ← phosphoglycerate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,064,9140GT99.1% 736.7 / ‑3.2 232I356I (ATC→ATA) pgkphosphoglycerate kinase
Reads supporting (aligned to +/- strand):  ref base G (3/0);  new base T (113/116);  total (116/116)
Fisher's exact test for biased strand distribution p-value = 2.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 233 total aligned reads displayed.

CTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGAGATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGG  >  CP009273/3064769‑3065057
                                                                                                                                                 |                                                                                                                                               
ctctAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGatat                                                                                                                                               <  1:1817852/148‑1 (MQ=255)
 tctAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATAtt                                                                                                                                              <  1:2371346/148‑1 (MQ=255)
 tctAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATAtt                                                                                                                                              <  1:3325183/148‑1 (MQ=255)
 tctAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGAGAtt                                                                                                                                              >  2:3494497/1‑148 (MQ=255)
  ctAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATAttt                                                                                                                                             >  2:1845392/1‑148 (MQ=255)
  ctAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATAttt                                                                                                                                             <  2:3672734/148‑1 (MQ=255)
    aaaaGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTg                                                                                                                                           >  2:289613/1‑148 (MQ=255)
       aGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCa                                                                                                                                        >  1:3431517/1‑148 (MQ=255)
        gcgcgcTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAg                                                                                                                                       <  1:805551/148‑1 (MQ=255)
        gcgcgcTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAg                                                                                                                                       >  2:1479126/1‑148 (MQ=255)
           cgcTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCaa                                                                                                                                    <  1:769795/148‑1 (MQ=255)
           cgcTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCaa                                                                                                                                    <  2:610220/148‑1 (MQ=255)
           cgcTGAAACAAGGGCAGGTGTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCaa                                                                                                                                    <  1:3655434/148‑1 (MQ=255)
             cTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATg                                                                                                                                  <  1:2689864/148‑1 (MQ=255)
              tGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGc                                                                                                                                 <  2:323912/148‑1 (MQ=255)
                 aaCAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGa                                                                                                                              <  2:2213392/148‑1 (MQ=255)
                   cAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAAc                                                                                                                            <  1:2590381/148‑1 (MQ=255)
                   cAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAAc                                                                                                                            <  1:2813588/148‑1 (MQ=255)
                    aaGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACa                                                                                                                           <  1:3724221/148‑1 (MQ=255)
                       ggCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGt                                                                                                                        >  1:527347/1‑148 (MQ=255)
                       ggCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGt                                                                                                                        >  1:1072254/1‑148 (MQ=255)
                       ggCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGt                                                                                                                        >  2:2602576/1‑148 (MQ=255)
                        gCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCCCGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTc                                                                                                                       >  1:1016011/1‑148 (MQ=255)
                           ggTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGAt                                                                                                                    <  1:3817398/148‑1 (MQ=255)
                           ggTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGAt                                                                                                                    <  2:2580010/148‑1 (MQ=255)
                           ggTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGAt                                                                                                                    >  1:3556194/1‑148 (MQ=255)
                           ggTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGAt                                                                                                                    >  1:170523/1‑148 (MQ=255)
                             tttCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATtg                                                                                                                  >  1:2793397/1‑148 (MQ=255)
                             tttCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATtg                                                                                                                  >  1:875757/1‑148 (MQ=255)
                                 cctgccctgTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATtgctgc                                                                                                              <  1:2971484/148‑1 (MQ=255)
                                 cctgccctgTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATtgctgc                                                                                                              <  2:122426/148‑1 (MQ=255)
                                       ctgTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAgt                                                                                                        >  2:2473816/1‑148 (MQ=255)
                                       ctgTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAgt                                                                                                        <  1:2145514/148‑1 (MQ=255)
                                            aTTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTcgc                                                                                                   >  2:809832/1‑148 (MQ=255)
                                            aTTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTcgc                                                                                                   >  2:2725924/1‑148 (MQ=255)
                                               ttttACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTcgccgc                                                                                                >  1:1768910/1‑148 (MQ=255)
                                                tttACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTcgccgcc                                                                                               >  2:3807323/1‑148 (MQ=255)
                                                 ttACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTcgccaccg                                                                                              >  1:845997/1‑148 (MQ=255)
                                                  tACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCTCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTcgccgccgc                                                                                             <  1:739575/148‑1 (MQ=255)
                                                   aCTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCgccgccgcc                                                                                            >  1:641015/1‑148 (MQ=255)
                                                     ttcttAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAg                                                                                          >  2:534915/1‑148 (MQ=255)
                                                      tcttAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGc                                                                                         >  1:1962954/1‑148 (MQ=255)
                                                        ttAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGa                                                                                       >  2:2085018/1‑148 (MQ=255)
                                                         tAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGAt                                                                                      >  1:3789533/1‑148 (MQ=255)
                                                           gcgcgcTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATgg                                                                                    >  1:3448326/1‑148 (MQ=255)
                                                            cgcgcTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGga                                                                                   >  2:1370751/1‑148 (MQ=255)
                                                                ctctTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAAc                                                                               >  2:208203/1‑148 (MQ=255)
                                                                ctctTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAAc                                                                               >  2:2637400/1‑148 (MQ=255)
                                                                  ctTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGc                                                                             <  1:3750199/148‑1 (MQ=255)
                                                                  ctTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGc                                                                             <  1:2469588/148‑1 (MQ=255)
                                                                   ttCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCt                                                                            <  1:321950/148‑1 (MQ=255)
                                                                      gAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCg                                                                         <  2:2466887/148‑1 (MQ=255)
                                                                      gAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCg                                                                         <  2:734807/148‑1 (MQ=255)
                                                                          aTCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGt                                                                     <  2:2302848/148‑1 (MQ=255)
                                                                           tCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTc                                                                    <  2:360946/148‑1 (MQ=255)
                                                                            cGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCCGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCt                                                                   >  2:3477904/1‑148 (MQ=255)
                                                                            cGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCt                                                                   >  2:1580395/1‑148 (MQ=255)
                                                                               tACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCg                                                                <  1:2727303/148‑1 (MQ=255)
                                                                                aCTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGa                                                               >  2:3076345/1‑148 (MQ=255)
                                                                                aCTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGAGGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGa                                                               >  2:2704845/1‑148 (MQ=255)
                                                                                 cTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGAt                                                              <  2:316874/148‑1 (MQ=255)
                                                                                 cTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGAt                                                              <  1:289461/148‑1 (MQ=255)
                                                                                 cTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGAt                                                              <  1:896218/148‑1 (MQ=255)
                                                                                      ggcagTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGt                                                         <  2:454899/148‑1 (MQ=255)
                                                                                       gcagTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGtt                                                        >  2:1652263/1‑148 (MQ=255)
                                                                                           tACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGcc                                                    >  2:2251430/1‑148 (MQ=255)
                                                                                            aCTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCa                                                   >  1:1423614/1‑148 (MQ=255)
                                                                                              tttACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACg                                                 >  2:64256/1‑148 (MQ=255)
                                                                                              tttACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACg                                                 >  1:1824560/1‑148 (MQ=255)
                                                                                                  ccTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGAttt                                             <  1:3022142/148‑1 (MQ=255)
                                                                                                  ccTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGAttt                                             <  1:1271961/148‑1 (MQ=255)
                                                                                                  ccTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGAttt                                             <  1:3024613/148‑1 (MQ=255)
                                                                                                      ccACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGt                                         <  2:3431517/148‑1 (MQ=255)
                                                                                                       cACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTa                                        <  2:3296914/148‑1 (MQ=255)
                                                                                                       cACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTa                                        <  2:3296981/148‑1 (MQ=255)
                                                                                                         cGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTAcc                                      >  2:1287871/1‑148 (MQ=255)
                                                                                                          gAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCt                                     <  1:2602576/148‑1 (MQ=255)
                                                                                                             ttCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTg                                  <  2:1094515/148‑1 (MQ=255)
                                                                                                             ttCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTg                                  <  2:1094413/148‑1 (MQ=255)
                                                                                                             ttCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTg                                  >  2:1033334/1‑148 (MQ=255)
                                                                                                                gAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCgg                               >  1:649245/1‑148 (MQ=255)
                                                                                                                   gAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAg                            <  1:1048584/148‑1 (MQ=255)
                                                                                                                   gAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAg                            >  1:3513272/1‑148 (MQ=255)
                                                                                                                    aaTGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGt                           <  2:1962954/148‑1 (MQ=255)
                                                                                                                    aaTGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGt                           <  2:2984002/148‑1 (MQ=255)
                                                                                                                     aTGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGtt                          <  2:2062249/148‑1 (MQ=255)
                                                                                                                         gccgccgccAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCggg                      <  1:2946696/148‑1 (MQ=255)
                                                                                                                           cgccgccAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGaa                    <  2:856133/148‑1 (MQ=255)
                                                                                                                                ccAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGa               <  1:1435514/148‑1 (MQ=255)
                                                                                                                                ccAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGa               <  1:1944700/148‑1 (MQ=255)
                                                                                                                                ccAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGa               <  1:1479137/148‑1 (MQ=255)
                                                                                                                                 cAGTGGAGATGTAGGATATTGTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGaa              <  2:1722247/148‑1 (MQ=255)
                                                                                                                                      gagaTGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGTGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGc         >  1:1554646/1‑148 (MQ=255)
                                                                                                                                      gagaTGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGc         <  2:2381847/148‑1 (MQ=255)
                                                                                                                                       agaTGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGcc        >  1:1134766/1‑148 (MQ=255)
                                                                                                                                           gTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAAcc    >  1:2735519/1‑148 (MQ=255)
                                                                                                                                           gTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGAATTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAAcc    >  2:3744417/1‑148 (MQ=255)
                                                                                                                                            tAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCg   >  1:2275867/1‑148 (MQ=255)
                                                                                                                                            tAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCg   >  2:985883/1‑148 (MQ=255)
                                                                                                                                             aGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCgg  >  1:3200153/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
CTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGAGATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGG  >  CP009273/3064769‑3065057

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGAGATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGT  >  CP009273/3064765‑3065062
                                                                                                                                                     |                                                                                                                                                    
CGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGAT                                                                                                                                                      <  SRR19973069.1354046/150‑1 (MQ=60)
CGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGAT                                                                                                                                                      <  SRR19973069.1353832/150‑1 (MQ=60)
 GTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATA                                                                                                                                                     <  SRR19973069.2932976/150‑1 (MQ=60)
 GTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATA                                                                                                                                                     >  SRR19973069.1529811/1‑150 (MQ=60)
 GTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATA                                                                                                                                                     >  SRR19973069.1207506/1‑150 (MQ=60)
 GTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGGGGAGATGTAGGATA                                                                                                                                                     >  SRR19973069.1524786/1‑150 (MQ=60)
    CTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTT                                                                                                                                                  <  SRR19973069.1830612/150‑1 (MQ=60)
     TCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTT                                                                                                                                                 <  SRR19973069.3346976/150‑1 (MQ=60)
     TCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTT                                                                                                                                                 <  SRR19973069.2387437/150‑1 (MQ=60)
      CTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTG                                                                                                                                                <  SRR19973069.2567561/150‑1 (MQ=60)
       TAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGT                                                                                                                                               <  SRR19973069.1830153/150‑1 (MQ=60)
        AAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTC                                                                                                                                              <  SRR19973069.2014081/150‑1 (MQ=60)
        AAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGAGATTTTGTC                                                                                                                                              >  SRR19973069.3758740/1‑150 (MQ=60)
         AAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCA                                                                                                                                             >  SRR19973069.3453823/1‑150 (MQ=60)
          AAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAG                                                                                                                                            <  SRR19973069.124444/150‑1 (MQ=60)
            GCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCA                                                                                                                                          <  SRR19973069.811263/150‑1 (MQ=60)
              GCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAAT                                                                                                                                        <  SRR19973069.2824270/150‑1 (MQ=60)
              GCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAAT                                                                                                                                        <  SRR19973069.40865/150‑1 (MQ=60)
               CGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATG                                                                                                                                       <  SRR19973069.775237/150‑1 (MQ=60)
               CGCTGAAACAAGGGCAGGTGTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATG                                                                                                                                       <  SRR19973069.3679373/150‑1 (MQ=60)
                 CTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCC                                                                                                                                     <  SRR19973069.2707884/150‑1 (MQ=60)
                 CTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCC                                                                                                                                     <  SRR19973069.778833/150‑1 (MQ=60)
                  TGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCG                                                                                                                                    >  SRR19973069.3003823/1‑150 (MQ=60)
                   GAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGA                                                                                                                                   <  SRR19973069.1971934/150‑1 (MQ=60)
                       CAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAG                                                                                                                               <  SRR19973069.2832226/150‑1 (MQ=60)
                       CAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAG                                                                                                                               <  SRR19973069.2832173/150‑1 (MQ=60)
                       CAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAG                                                                                                                               <  SRR19973069.2607645/150‑1 (MQ=60)
                        AAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGG                                                                                                                              <  SRR19973069.2373525/150‑1 (MQ=60)
                        AAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGG                                                                                                                              <  SRR19973069.3748525/150‑1 (MQ=60)
                         AGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGT                                                                                                                             >  SRR19973069.531072/1‑150 (MQ=60)
                         AGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGT                                                                                                                             >  SRR19973069.2978226/1‑150 (MQ=60)
                         AGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGT                                                                                                                             >  SRR19973069.1079733/1‑150 (MQ=60)
                         AGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGT                                                                                                                             >  SRR19973069.1079109/1‑150 (MQ=60)
                         AGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGT                                                                                                                             <  SRR19973069.3828282/150‑1 (MQ=60)
                          GGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCCCGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTC                                                                                                                            >  SRR19973069.1023021/1‑150 (MQ=60)
                             CAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGAT                                                                                                                         >  SRR19973069.171986/1‑150 (MQ=60)
                             CAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGAT                                                                                                                         >  SRR19973069.171978/1‑150 (MQ=60)
                             CAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGAT                                                                                                                         >  SRR19973069.3579326/1‑150 (MQ=60)
                              AGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATT                                                                                                                        <  SRR19973069.53234/150‑1 (MQ=60)
                               GGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTG                                                                                                                       >  SRR19973069.2811969/1‑150 (MQ=60)
                               GGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTG                                                                                                                       >  SRR19973069.2940729/1‑150 (MQ=60)
                               GGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTG                                                                                                                       >  SRR19973069.881842/1‑150 (MQ=60)
                               GGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTG                                                                                                                       <  SRR19973069.3842102/150‑1 (MQ=60)
                                 TTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCT                                                                                                                     <  SRR19973069.3230647/150‑1 (MQ=60)
                                     CCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCA                                                                                                                 <  SRR19973069.721733/150‑1 (MQ=60)
                                     CCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCA                                                                                                                 <  SRR19973069.2991211/150‑1 (MQ=60)
                                         CCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGT                                                                                                             <  SRR19973069.1604485/150‑1 (MQ=60)
                                          CCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTG                                                                                                            >  SRR19973069.2602423/1‑150 (MQ=60)
                                          CCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTG                                                                                                            <  SRR19973069.3379109/150‑1 (MQ=60)
                                           CTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGT                                                                                                           <  SRR19973069.2160233/150‑1 (MQ=60)
                                           CTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGT                                                                                                           >  SRR19973069.2747855/1‑150 (MQ=60)
                                               GATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCC                                                                                                       <  SRR19973069.1458562/150‑1 (MQ=60)
                                                 TTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGC                                                                                                     >  SRR19973069.1781378/1‑150 (MQ=60)
                                                 TTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGC                                                                                                     >  SRR19973069.1779029/1‑150 (MQ=60)
                                                   TTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCACCG                                                                                                   >  SRR19973069.852043/1‑150 (MQ=60)
                                                   TTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCACCG                                                                                                   >  SRR19973069.851928/1‑150 (MQ=60)
                                                    TTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGC                                                                                                  >  SRR19973069.194591/1‑150 (MQ=60)
                                                     TTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCC                                                                                                 >  SRR19973069.645223/1‑150 (MQ=60)
                                                     TTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCC                                                                                                 >  SRR19973069.645391/1‑150 (MQ=60)
                                                     TTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCC                                                                                                 >  SRR19973069.899572/1‑150 (MQ=60)
                                                      TACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCTCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCA                                                                                                <  SRR19973069.744816/150‑1 (MQ=60)
                                                        CTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGC                                                                                              >  SRR19973069.1976564/1‑150 (MQ=60)
                                                           CTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGAT                                                                                           >  SRR19973069.3813991/1‑150 (MQ=60)
                                                           CTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGAT                                                                                           >  SRR19973069.3814108/1‑150 (MQ=60)
                                                             TAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGG                                                                                         >  SRR19973069.3470196/1‑150 (MQ=60)
                                                             TAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGG                                                                                         >  SRR19973069.3470690/1‑150 (MQ=60)
                                                                  CGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAAC                                                                                    >  SRR19973069.702348/1‑150 (MQ=60)
                                                                  CGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAAC                                                                                    <  SRR19973069.748953/150‑1 (MQ=60)
                                                                  CGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAAC                                                                                    >  SRR19973069.2632895/1‑150 (MQ=60)
                                                                  CGCTCTTCGAGCATCGCTACTGCAGGCAGGACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAAC                                                                                    <  SRR19973069.283431/150‑1 (MQ=60)
                                                                      CTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTT                                                                                <  SRR19973069.3774615/150‑1 (MQ=60)
                                                                      CTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTT                                                                                <  SRR19973069.2486159/150‑1 (MQ=60)
                                                                       TTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTC                                                                               <  SRR19973069.324081/150‑1 (MQ=60)
                                                                                  CTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGA                                                                    >  SRR19973069.1230961/1‑150 (MQ=60)
                                                                                  CTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGA                                                                    >  SRR19973069.3147731/1‑150 (MQ=60)
                                                                                   TACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATTTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGAT                                                                   <  SRR19973069.2745568/150‑1 (MQ=60)
                                                                                   TACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGAT                                                                   <  SRR19973069.815566/150‑1 (MQ=60)
                                                                                   TACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGAT                                                                   <  SRR19973069.2744183/150‑1 (MQ=60)
                                                                                     CTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAG                                                                 <  SRR19973069.902394/150‑1 (MQ=60)
                                                                                     CTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAG                                                                 <  SRR19973069.291458/150‑1 (MQ=60)
                                                                                             AGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCC                                                         >  SRR19973069.3427869/1‑150 (MQ=60)
                                                                                              GTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCA                                                        >  SRR19973069.1433564/1‑150 (MQ=60)
                                                                                                ACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACG                                                      >  SRR19973069.83023/1‑150 (MQ=60)
                                                                                                ACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACG                                                      >  SRR19973069.3560010/1‑150 (MQ=60)
                                                                                                ACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACG                                                      >  SRR19973069.3559317/1‑150 (MQ=60)
                                                                                                ACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACG                                                      >  SRR19973069.1837361/1‑150 (MQ=60)
                                                                                                ACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACG                                                      >  SRR19973069.1837169/1‑150 (MQ=60)
                                                                                                  TTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGAT                                                    <  SRR19973069.546572/150‑1 (MQ=60)
                                                                                                  TTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGAT                                                    <  SRR19973069.2726505/150‑1 (MQ=60)
                                                                                                     ACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCNATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTC                                                 >  SRR19973069.1946203/1‑150 (MQ=60)
                                                                                                      CCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCA                                                <  SRR19973069.3044669/150‑1 (MQ=60)
                                                                                                      CCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCA                                                <  SRR19973069.3044679/150‑1 (MQ=60)
                                                                                                      CCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCA                                                <  SRR19973069.3042184/150‑1 (MQ=60)
                                                                                                      CCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCA                                                <  SRR19973069.1280506/150‑1 (MQ=60)
                                                                                                       CTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAG                                               >  SRR19973069.2801713/1‑150 (MQ=60)
                                                                                                          CCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTAC                                            >  SRR19973069.1358677/1‑150 (MQ=60)
                                                                                                            ACGACTTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTGTGTCAGCAATGCCTAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGAGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCT                                          <  SRR19973069.3678501/150‑1 (MQ=60)
                                                                                                              GAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTT                                        <  SRR19973069.2619954/150‑1 (MQ=60)
                                                                                                              GAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTT                                        <  SRR19973069.3488575/150‑1 (MQ=60)
                                                                                                               AATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTG                                       >  SRR19973069.3156168/1‑150 (MQ=60)
                                                                                                                ATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGC                                      >  SRR19973069.1036577/1‑150 (MQ=60)
                                                                                                                  TCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGG                                    >  SRR19973069.653548/1‑150 (MQ=60)
                                                                                                                  TCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCAGAACAGGTCGATGTCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGG                                    >  SRR19973069.3770134/1‑150 (MQ=60)
                                                                                                                     AGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAG                                 >  SRR19973069.3535924/1‑150 (MQ=60)
                                                                                                                      GGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGT                                <  SRR19973069.2444076/150‑1 (MQ=60)
                                                                                                                       GAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTT                               <  SRR19973069.1055860/150‑1 (MQ=60)
                                                                                                                             GCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAA                         <  SRR19973069.2966254/150‑1 (MQ=60)
                                                                                                                             GCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAA                         <  SRR19973069.2966287/150‑1 (MQ=60)
                                                                                                                               CGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATT                       <  SRR19973069.1108144/150‑1 (MQ=60)
                                                                                                                                 CCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCG                     <  SRR19973069.3731989/150‑1 (MQ=60)
                                                                                                                                 CCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCG                     <  SRR19973069.3254781/150‑1 (MQ=60)
                                                                                                                                    CCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAAC                  >  SRR19973069.2190973/1‑150 (MQ=60)
                                                                                                                                    CCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAAC                  <  SRR19973069.1958110/150‑1 (MQ=60)
                                                                                                                                    CCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAAC                  <  SRR19973069.1489428/150‑1 (MQ=60)
                                                                                                                                    CCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAAC                  <  SRR19973069.1489417/150‑1 (MQ=60)
                                                                                                                                    CCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAAC                  <  SRR19973069.1445526/150‑1 (MQ=60)
                                                                                                                                    CCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAAC                  <  SRR19973069.1445484/150‑1 (MQ=60)
                                                                                                                                        TGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGTGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGC              >  SRR19973069.1565270/1‑150 (MQ=60)
                                                                                                                                        TGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGC              >  SRR19973069.1565131/1‑150 (MQ=60)
                                                                                                                                         GGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCC             >  SRR19973069.1142689/1‑150 (MQ=60)
                                                                                                                                         GGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCC             <  SRR19973069.2613001/150‑1 (MQ=60)
                                                                                                                                             ATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACC         >  SRR19973069.2753821/1‑150 (MQ=60)
                                                                                                                                              TGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCG        >  SRR19973069.2291312/1‑150 (MQ=60)
                                                                                                                                               GTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGG       >  SRR19973069.3220962/1‑150 (MQ=60)
                                                                                                                                                 AGGATATTTTGTCATCATTGCCGAACAGGTCTTTTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGATTCGCTGTGTGCGATAGCGTTAGCCACGATTTCTGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGAC     <  SRR19973069.325873/150‑1 (MQ=60)
                                                                                                                                                 AGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGAC     <  SRR19973069.2266623/150‑1 (MQ=60)
                                                                                                                                                   GATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCG   >  SRR19973069.3773366/1‑150 (MQ=60)
                                                                                                                                                    ATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGT  >  SRR19973069.3215454/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
CGTGCTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGAGATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGACCGT  >  CP009273/3064765‑3065062

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑