Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,134,528 C→A 86.0% D40Y (GAT→TAT)  hybD ← maturation protease for hydrogenase 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,134,5280CA86.0% 417.4 / 72.5 186D40Y (GAT→TAT) hybDmaturation protease for hydrogenase 2
Reads supporting (aligned to +/- strand):  ref base C (13/13);  new base A (76/84);  total (89/97)
Fisher's exact test for biased strand distribution p-value = 8.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

Only 100 of 186 total aligned reads displayed.

GGTAAACAACGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTT  >  CP009273/3134385‑3134674
                                                                                                                                               |                                                                                                                                                  
ggTAAACAACGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAgg                                                                                                                                                <  2:3183782/148‑1 (MQ=255)
  tAAACAACGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGAt                                                                                                                                              <  2:330318/148‑1 (MQ=255)
  tAAACAACGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGAt                                                                                                                                              <  2:413853/148‑1 (MQ=255)
    aacaacGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGAtct                                                                                                                                            >  2:859389/1‑148 (MQ=255)
    aacaacGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGAtct                                                                                                                                            <  2:2172108/148‑1 (MQ=255)
    aacaacGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGAtct                                                                                                                                            >  1:1764575/1‑148 (MQ=255)
     acaacGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGAtctc                                                                                                                                           >  1:2000495/1‑148 (MQ=255)
      caacGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATTATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCa                                                                                                                                          >  2:1668197/1‑148 (MQ=255)
      caacGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCa                                                                                                                                          >  1:2668744/1‑148 (MQ=255)
      caacGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCa                                                                                                                                          <  2:1209825/148‑1 (MQ=255)
      caacGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCa                                                                                                                                          <  2:2023968/148‑1 (MQ=255)
       aacGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCaa                                                                                                                                         >  2:1647281/1‑148 (MQ=255)
         cGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACa                                                                                                                                       >  2:2823651/1‑148 (MQ=255)
         cGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACa                                                                                                                                       >  1:521740/1‑148 (MQ=255)
         cGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACa                                                                                                                                       >  1:521595/1‑148 (MQ=255)
          gCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACAt                                                                                                                                      <  2:2493719/148‑1 (MQ=255)
            cGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATaa                                                                                                                                    <  2:1105730/148‑1 (MQ=255)
             ggAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAAt                                                                                                                                   <  2:1360792/148‑1 (MQ=255)
              gAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATc                                                                                                                                  >  1:2232189/1‑148 (MQ=255)
               aaCTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATcc                                                                                                                                 >  1:2636649/1‑148 (MQ=255)
               aaCTTCGTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATcc                                                                                                                                 <  2:2266576/148‑1 (MQ=255)
                  ttcttcATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAACTGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGc                                                                                                                              <  1:2498800/148‑1 (MQ=255)
                     ttcATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGTCGCCATAGAGGATCTCAACATAATCCGGCAGa                                                                                                                           <  2:198737/148‑1 (MQ=255)
                     ttcATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGa                                                                                                                           <  1:2750284/148‑1 (MQ=255)
                      tcATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGaa                                                                                                                          >  1:955135/1‑148 (MQ=255)
                      tcATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGaa                                                                                                                          <  2:2143389/148‑1 (MQ=255)
                      tcATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGaa                                                                                                                          >  1:2451967/1‑148 (MQ=255)
                        aTCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATg                                                                                                                        >  1:1929974/1‑148 (MQ=255)
                         tCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGt                                                                                                                       <  1:1034631/148‑1 (MQ=255)
                         tCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGt                                                                                                                       <  1:2878074/148‑1 (MQ=255)
                         tCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGt                                                                                                                       <  2:247355/148‑1 (MQ=255)
                           ccGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTAt                                                                                                                     <  1:2508562/148‑1 (MQ=255)
                             gCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCg                                                                                                                   <  2:2730070/148‑1 (MQ=255)
                             gCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCg                                                                                                                   <  2:1078743/148‑1 (MQ=255)
                              cAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGt                                                                                                                  >  1:701962/1‑148 (MQ=255)
                                   tcatcatcGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGctc                                                                                                             <  1:2457630/148‑1 (MQ=255)
                                   tcatcatcGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGctc                                                                                                             <  1:1572060/148‑1 (MQ=255)
                                    catcatcGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGctct                                                                                                            >  1:893219/1‑148 (MQ=255)
                                       catcGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAAc                                                                                                         >  2:859625/1‑148 (MQ=255)
                                         tcGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGc                                                                                                       >  2:2897382/1‑148 (MQ=255)
                                          cGTTCCCGGCGCGTTCTTTTTCGAGACAATTGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCt                                                                                                      >  1:108915/1‑148 (MQ=255)
                                          cGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCt                                                                                                      >  1:227232/1‑148 (MQ=255)
                                              cccGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGa                                                                                                  <  1:1540306/148‑1 (MQ=255)
                                                     cgTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCg                                                                                           >  2:3223128/1‑148 (MQ=255)
                                                     cgTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCg                                                                                           >  2:3223138/1‑148 (MQ=255)
                                                     cgTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCg                                                                                           >  2:3104037/1‑148 (MQ=255)
                                                      gTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGc                                                                                          <  2:227232/148‑1 (MQ=255)
                                                        tCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGcac                                                                                        >  1:1848129/1‑148 (MQ=255)
                                                         cTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGcaca                                                                                       >  2:579516/1‑148 (MQ=255)
                                                          tttttCGAGACAATTGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCATCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCacac                                                                                      <  1:755438/148‑1 (MQ=255)
                                                          tttttCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCacac                                                                                      <  2:2390077/148‑1 (MQ=255)
                                                           ttatCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAATCAGCTCCATTCCCGCCGATCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACACGCCGCACAcc                                                                                     >  2:1270655/4‑148 (MQ=255)
                                                            tttCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAAGATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCg                                                                                    <  1:1419543/148‑1 (MQ=255)
                                                            tttCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCg                                                                                    <  1:863401/148‑1 (MQ=255)
                                                             ttCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGa                                                                                   >  1:2089907/1‑148 (MQ=255)
                                                               cGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATg                                                                                 >  1:1501862/1‑148 (MQ=255)
                                                               cGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATg                                                                                 >  1:1980602/1‑148 (MQ=255)
                                                                 agaCAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGc                                                                               <  1:10872/148‑1 (MQ=255)
                                                                   aCAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCtt                                                                             >  2:428561/1‑148 (MQ=255)
                                                                   aCAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCtt                                                                             >  2:3003362/1‑148 (MQ=255)
                                                                    cAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTc                                                                            <  1:2008710/148‑1 (MQ=255)
                                                                     aaTGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCa                                                                           <  2:2061345/148‑1 (MQ=255)
                                                                     aaTGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCa                                                                           <  2:2183371/148‑1 (MQ=255)
                                                                      aTGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCAt                                                                          <  1:590881/148‑1 (MQ=255)
                                                                      aTGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCAt                                                                          <  1:234109/148‑1 (MQ=255)
                                                                           aTCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTc                                                                     <  2:1450295/148‑1 (MQ=255)
                                                                           aTCCGCAATAATCAAATGATCGCGATGTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTc                                                                     <  2:3093672/148‑1 (MQ=255)
                                                                             ccGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAg                                                                   >  2:1527087/1‑148 (MQ=255)
                                                                             ccGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAg                                                                   >  2:1018301/1‑148 (MQ=255)
                                                                              cGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGc                                                                  >  1:1348481/1‑148 (MQ=255)
                                                                                   taatCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAat                                                             <  2:2118067/148‑1 (MQ=255)
                                                                                   taatCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAat                                                             <  2:2949459/148‑1 (MQ=255)
                                                                                     atCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAatat                                                           >  2:922832/1‑148 (MQ=255)
                                                                                          aTGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCg                                                      >  1:1868708/1‑148 (MQ=255)
                                                                                          aTGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCg                                                      <  2:134962/148‑1 (MQ=255)
                                                                                          aTGATCGCGATTTGCCATGTCGCCAAGCAGCTCCAGTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCg                                                      <  2:1694672/148‑1 (MQ=255)
                                                                                           tGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGa                                                     >  2:2549191/1‑148 (MQ=255)
                                                                                           tGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGa                                                     >  2:735759/1‑148 (MQ=255)
                                                                                              tCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGAccc                                                  <  2:924684/148‑1 (MQ=255)
                                                                                              tCGCGATTTGCCATGTCGCAAAGCAGCTCCATTCCCGCCGTGCCGCCATAGATGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTATCGCTTCGACATTCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGAccc                                                  <  2:3004383/148‑1 (MQ=255)
                                                                                               cgcgATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACTGATGGCTTCATCGGTCAGCAAAATATTGCCGAcccc                                                 <  2:701962/148‑1 (MQ=255)
                                                                                                       gCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTa                                         >  1:2966374/1‑148 (MQ=255)
                                                                                                         cATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTaaa                                       <  2:2636649/148‑1 (MQ=255)
                                                                                                         cATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTaaa                                       >  1:2981895/1‑148 (MQ=255)
                                                                                                               gCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGc                                 <  2:29533/148‑1 (MQ=255)
                                                                                                                ccAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCa                                <  2:1764575/148‑1 (MQ=255)
                                                                                                                ccAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCa                                <  2:90352/148‑1 (MQ=255)
                                                                                                                 cAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCAt                               >  2:1232721/1‑148 (MQ=255)
                                                                                                                           cATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCt                     <  1:3260287/148‑1 (MQ=255)
                                                                                                                           cATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCt                     <  1:3260037/148‑1 (MQ=255)
                                                                                                                            aTTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCtt                    >  1:2848909/1‑148 (MQ=255)
                                                                                                                            aTTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCtt                    >  1:2691713/1‑148 (MQ=255)
                                                                                                                             ttCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTc                   >  2:1744197/1‑148 (MQ=255)
                                                                                                                             ttCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTc                   <  1:2975367/148‑1 (MQ=255)
                                                                                                                             ttCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTc                   <  1:1063929/148‑1 (MQ=255)
                                                                                                                                ccgccgTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACt                <  1:3140195/148‑1 (MQ=255)
                                                                                                                                 cgccgTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTg               <  1:2823651/148‑1 (MQ=255)
                                                                                                                                       gccgccATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCa         <  2:521595/148‑1 (MQ=255)
                                                                                                                                       gccgccATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCa         <  2:521740/148‑1 (MQ=255)
                                                                                                                                          gccATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACtt      <  2:108915/148‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                  
GGTAAACAACGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTT  >  CP009273/3134385‑3134674

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTTGGTAAACAACGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCC  >  CP009273/3134382‑3134677
                                                                                                                                                  |                                                                                                                                                     
GTTGGTAAACAACGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAG                                                                                                                                                    <  SRR19973070.1270932/150‑1 (MQ=60)
     TAAACAACGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCT                                                                                                                                               >  SRR19973070.91256/1‑150 (MQ=60)
     TAAACAACGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCT                                                                                                                                               >  SRR19973070.1776783/1‑150 (MQ=60)
      AAACAACGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTC                                                                                                                                              >  SRR19973070.2014046/1‑150 (MQ=60)
       AACAACGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCA                                                                                                                                             >  SRR19973070.1922554/1‑150 (MQ=60)
       AACAACGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCA                                                                                                                                             >  SRR19973070.2685988/1‑150 (MQ=60)
       AACAACGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCA                                                                                                                                             >  SRR19973070.2686079/1‑150 (MQ=60)
       AACAACGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTATTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGAGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCA                                                                                                                                             >  SRR19973070.2686108/1‑150 (MQ=60)
        ACAACGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAA                                                                                                                                            <  SRR19973070.3008924/150‑1 (MQ=60)
          AACGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCCTAGAGGATCTCAACA                                                                                                                                          >  SRR19973070.1138310/1‑150 (MQ=60)
          AACGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACA                                                                                                                                          >  SRR19973070.525279/1‑150 (MQ=60)
          AACGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACA                                                                                                                                          >  SRR19973070.525140/1‑150 (MQ=60)
          AACGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACA                                                                                                                                          >  SRR19973070.525286/1‑150 (MQ=60)
          AACGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACA                                                                                                                                          >  SRR19973070.525301/1‑150 (MQ=60)
               CGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATC                                                                                                                                     >  SRR19973070.2246901/1‑150 (MQ=60)
                GGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCC                                                                                                                                    >  SRR19973070.2653836/1‑150 (MQ=60)
                 GAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCG                                                                                                                                   <  SRR19973070.1776968/150‑1 (MQ=60)
                    CTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCA                                                                                                                                <  SRR19973070.1426043/150‑1 (MQ=60)
                     TTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAACTGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAG                                                                                                                               <  SRR19973070.2515012/150‑1 (MQ=60)
                       CTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAA                                                                                                                             >  SRR19973070.961953/1‑150 (MQ=60)
                       CTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAA                                                                                                                             >  SRR19973070.2468006/1‑150 (MQ=60)
                       CTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTATTTCGAGACAATGTCATCCGCACTAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGATCAATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAA                                                                                                                             >  SRR19973070.2468377/1‑150 (MQ=60)
                        TTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAAT                                                                                                                            <  SRR19973070.2764605/150‑1 (MQ=60)
                        TTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAAT                                                                                                                            <  SRR19973070.2767951/150‑1 (MQ=60)
                         TCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATG                                                                                                                           >  SRR19973070.1942973/1‑150 (MQ=60)
                            TCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTAT                                                                                                                        <  SRR19973070.2896715/150‑1 (MQ=60)
                            TCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTAT                                                                                                                        <  SRR19973070.1041944/150‑1 (MQ=60)
                              CCGCAGGATCATCCTCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTATCG                                                                                                                      <  SRR19973070.2524844/150‑1 (MQ=60)
                              CCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTATCG                                                                                                                      <  SRR19973070.2524878/150‑1 (MQ=60)
                              CCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCG                                                                                                                      <  SRR19973070.2124191/150‑1 (MQ=60)
                               CGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGT                                                                                                                     >  SRR19973070.706965/1‑150 (MQ=60)
                                     ATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCT                                                                                                               >  SRR19973070.899534/1‑150 (MQ=60)
                                      TCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTA                                                                                                              <  SRR19973070.1583103/150‑1 (MQ=60)
                                      TCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTA                                                                                                              <  SRR19973070.2473688/150‑1 (MQ=60)
                                        ATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAAC                                                                                                            <  SRR19973070.991368/150‑1 (MQ=60)
                                           ATCGTTCCCGGCGCGTTCTTTTTCGAGACAATTGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCT                                                                                                         >  SRR19973070.109925/1‑150 (MQ=60)
                                           ATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATTATAGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCT                                                                                                         >  SRR19973070.637633/1‑150 (MQ=60)
                                           ATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCT                                                                                                         >  SRR19973070.228851/1‑150 (MQ=60)
                                           ATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCT                                                                                                         <  SRR19973070.2263419/150‑1 (MQ=60)
                                              GTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCG                                                                                                      <  SRR19973070.2004637/150‑1 (MQ=60)
                                              GTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCG                                                                                                      >  SRR19973070.1096643/1‑150 (MQ=60)
                                                 CCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACA                                                                                                   <  SRR19973070.1551131/150‑1 (MQ=60)
                                                   CGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAAT                                                                                                 >  SRR19973070.772321/1‑150 (MQ=60)
                                                         GTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCAC                                                                                           >  SRR19973070.1860803/1‑150 (MQ=60)
                                                             TTTTTCGAGACAATTGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCATCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCG                                                                                       <  SRR19973070.760766/150‑1 (MQ=60)
                                                              TTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGA                                                                                      >  SRR19973070.2103961/1‑150 (MQ=60)
                                                               TTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAAGATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGAT                                                                                     <  SRR19973070.1429280/150‑1 (MQ=60)
                                                               TTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGAT                                                                                     <  SRR19973070.1989020/150‑1 (MQ=60)
                                                               TTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGAT                                                                                     <  SRR19973070.869411/150‑1 (MQ=60)
                                                                TTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATG                                                                                    >  SRR19973070.1994000/1‑150 (MQ=60)
                                                                TTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATG                                                                                    >  SRR19973070.1512378/1‑150 (MQ=60)
                                                                   GAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCT                                                                                 >  SRR19973070.1076292/1‑150 (MQ=60)
                                                                    AGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTT                                                                                <  SRR19973070.11045/150‑1 (MQ=60)
                                                                       CAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCAT                                                                             <  SRR19973070.2022308/150‑1 (MQ=60)
                                                                         ATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCG                                                                           <  SRR19973070.235754/150‑1 (MQ=60)
                                                                         ATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCG                                                                           <  SRR19973070.594967/150‑1 (MQ=60)
                                                                          TGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGG                                                                          >  SRR19973070.753560/1‑150 (MQ=60)
                                                                             CATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCA                                                                       <  SRR19973070.2973660/150‑1 (MQ=60)
                                                                               TCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGC                                                                     >  SRR19973070.1357727/1‑150 (MQ=60)
                                                                                       AATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATT                                                             >  SRR19973070.1667252/1‑150 (MQ=60)
                                                                                        ATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTG                                                            <  SRR19973070.346430/150‑1 (MQ=60)
                                                                                           AAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCG                                                         >  SRR19973070.1881462/1‑150 (MQ=60)
                                                                                           AAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCG                                                         >  SRR19973070.1881464/1‑150 (MQ=60)
                                                                                                        TTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTA                                            >  SRR19973070.2985440/1‑150 (MQ=60)
                                                                                                        TTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTA                                            >  SRR19973070.2985441/1‑150 (MQ=60)
                                                                                                          GCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAA                                          >  SRR19973070.3001028/1‑150 (MQ=60)
                                                                                                                  GCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCAT                                  <  SRR19973070.2879649/150‑1 (MQ=60)
                                                                                                                   CCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAATACTAAAATACGCATT                                 <  SRR19973070.2997037/150‑1 (MQ=60)
                                                                                                                   CCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATT                                 <  SRR19973070.2082684/150‑1 (MQ=60)
                                                                                                                     AAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTAC                               >  SRR19973070.2789818/1‑150 (MQ=60)
                                                                                                                           CTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACC                         >  SRR19973070.2567382/1‑150 (MQ=60)
                                                                                                                             CCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTT                       >  SRR19973070.2867359/1‑150 (MQ=60)
                                                                                                                             CCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTT                       >  SRR19973070.2709149/1‑150 (MQ=60)
                                                                                                                              CATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTC                      <  SRR19973070.3281279/150‑1 (MQ=60)
                                                                                                                              CATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTC                      <  SRR19973070.3281029/150‑1 (MQ=60)
                                                                                                                                TTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCAC                    <  SRR19973070.2994469/150‑1 (MQ=60)
                                                                                                                                TTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCAC                    <  SRR19973070.1071387/150‑1 (MQ=60)
                                                                                                                                   CCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGA                 <  SRR19973070.1509370/150‑1 (MQ=60)
                                                                                                                                   CCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGA                 <  SRR19973070.3160484/150‑1 (MQ=60)
                                                                                                                                    CGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAA                <  SRR19973070.2841915/150‑1 (MQ=60)
                                                                                                                                       CGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACC             >  SRR19973070.230541/1‑150 (MQ=60)
                                                                                                                                          GCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACT          <  SRR19973070.2377119/150‑1 (MQ=60)
                                                                                                                                          GCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACT          <  SRR19973070.1516848/150‑1 (MQ=60)
                                                                                                                                               CATCGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTT     >  SRR19973070.3217735/1‑150 (MQ=60)
                                                                                                                                                  AGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCC  >  SRR19973070.1677381/1‑150 (MQ=60)
                                                                                                                                                  AGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCC  >  SRR19973070.1677398/1‑150 (MQ=60)
                                                                                                                                                  AGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCC  >  SRR19973070.2676266/1‑150 (MQ=60)
                                                                                                                                                  |                                                                                                                                                     
GTTGGTAAACAACGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCC  >  CP009273/3134382‑3134677

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑