Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,134,528 C→A 100% D40Y (GAT→TAT)  hybD ← maturation protease for hydrogenase 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,134,5280CA98.7% 521.1 / ‑1.6 170D40Y (GAT→TAT) hybDmaturation protease for hydrogenase 2
Reads supporting (aligned to +/- strand):  ref base C (2/1);  new base A (70/97);  total (72/98)
Fisher's exact test for biased strand distribution p-value = 5.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 175 total aligned reads displayed.

TTGGTAAACAACGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTC  >  CP009273/3134383‑3134671
                                                                                                                                                 |                                                                                                                                               
ttgGTAAACAACGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGa                                                                                                                                               >  1:1368057/1‑148 (MQ=255)
 tgGTAAACAACGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATagag                                                                                                                                              >  2:1761102/1‑148 (MQ=255)
 tgGTAAACAACGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATagag                                                                                                                                              >  2:3526657/1‑148 (MQ=255)
 tgGTAAACAACGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATagag                                                                                                                                              >  2:1291590/1‑148 (MQ=255)
 tgGTAAACAACGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATagag                                                                                                                                              >  2:1761039/1‑148 (MQ=255)
   gTAAACAACGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGa                                                                                                                                            <  1:3559774/148‑1 (MQ=255)
   gTAAACAACGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGa                                                                                                                                            <  2:138971/148‑1 (MQ=255)
   gTAAACAACGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGa                                                                                                                                            <  1:609657/148‑1 (MQ=255)
    tAAACAACGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGAt                                                                                                                                           >  2:442592/1‑148 (MQ=255)
    tAAACAACGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGAt                                                                                                                                           <  1:1831230/148‑1 (MQ=255)
     aaaCACCGCCGGAACGTCGCCATCCCGCAGGATCATCATCGGTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATACTCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGAtc                                                                                                                                          <  2:3394381/148‑1 (MQ=255)
      aacaacGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGAtct                                                                                                                                         <  2:1872546/148‑1 (MQ=255)
        caacGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCa                                                                                                                                       >  2:3257653/1‑148 (MQ=255)
             ccGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATa                                                                                                                                  <  1:1271388/148‑1 (MQ=255)
             ccGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATa                                                                                                                                  <  1:1814582/148‑1 (MQ=255)
                gAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATc                                                                                                                               >  2:3124458/1‑148 (MQ=255)
                  aCTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTGTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCg                                                                                                                             <  2:2968109/148‑1 (MQ=255)
                      cttcATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAg                                                                                                                         >  2:60399/1‑148 (MQ=255)
                      cttcATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAg                                                                                                                         >  1:2710854/1‑148 (MQ=255)
                       ttcATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGa                                                                                                                        <  1:155327/148‑1 (MQ=255)
                        tcATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGaa                                                                                                                       <  2:2251215/148‑1 (MQ=255)
                          aTCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATg                                                                                                                     <  1:800601/148‑1 (MQ=255)
                           tCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGt                                                                                                                    <  2:1920985/148‑1 (MQ=255)
                            cccGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTa                                                                                                                   >  2:371432/1‑148 (MQ=255)
                             ccGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTAt                                                                                                                  <  2:2989714/148‑1 (MQ=255)
                               gCAGGATCATCATCGGTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCg                                                                                                                <  2:1785532/148‑1 (MQ=255)
                                    atcatcatcGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGct                                                                                                           <  1:926113/148‑1 (MQ=255)
                                      catcatcGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGctct                                                                                                         >  1:2389572/1‑148 (MQ=255)
                                          atcGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACg                                                                                                     <  1:2969543/148‑1 (MQ=255)
                                           tcGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGc                                                                                                    <  2:2478202/148‑1 (MQ=255)
                                            cGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCt                                                                                                   <  2:3220951/148‑1 (MQ=255)
                                            cGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCt                                                                                                   >  2:1487323/1‑148 (MQ=255)
                                            cGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCt                                                                                                   <  2:3220406/148‑1 (MQ=255)
                                            cGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCAGAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCt                                                                                                   >  1:3424418/1‑148 (MQ=255)
                                             gTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCtt                                                                                                  <  1:174124/148‑1 (MQ=255)
                                              ttCCCGGCGCGTTCTTTTTCGAGACAATGGCATACGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCCCCGTGCCGCCCTAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTc                                                                                                 >  1:1054960/1‑148 (MQ=255)
                                                cccGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATGTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGa                                                                                               <  1:1155779/148‑1 (MQ=255)
                                                  cGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACa                                                                                             <  1:1305796/148‑1 (MQ=255)
                                                   ggCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACaa                                                                                            <  1:3257653/148‑1 (MQ=255)
                                                     cgcgTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATc                                                                                          >  2:2693151/1‑148 (MQ=255)
                                                     cgcgTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATc                                                                                          >  2:913009/1‑148 (MQ=255)
                                                         ttCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGca                                                                                      >  1:3405977/1‑148 (MQ=255)
                                                         ttCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCAGTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGca                                                                                      <  2:2829253/148‑1 (MQ=255)
                                                          tCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGcac                                                                                     >  1:3500043/1‑148 (MQ=255)
                                                          tCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGcac                                                                                     >  1:2020182/1‑148 (MQ=255)
                                                           cTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGcaca                                                                                    >  2:2856016/1‑148 (MQ=255)
                                                              tttCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCg                                                                                 <  2:1702330/148‑1 (MQ=255)
                                                               ttCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGa                                                                                <  1:2696895/148‑1 (MQ=255)
                                                                 cGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCACCATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATg                                                                              <  1:3408307/148‑1 (MQ=255)
                                                                  gagaCAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATgg                                                                             <  2:801591/148‑1 (MQ=255)
                                                                           gCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCgg                                                                    >  1:921027/1‑148 (MQ=255)
                                                                           gCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCgg                                                                    >  1:504593/1‑148 (MQ=255)
                                                                               ccGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAg                                                                >  1:1645771/1‑148 (MQ=255)
                                                                                 gCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCa                                                              <  1:60399/148‑1 (MQ=255)
                                                                                  cAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCaa                                                             >  1:3217039/1‑148 (MQ=255)
                                                                                  cAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCaa                                                             >  1:1444457/1‑148 (MQ=255)
                                                                                   aataatCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCaaa                                                            <  1:2924782/148‑1 (MQ=255)
                                                                                    ataatCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCaaaa                                                           <  1:637286/148‑1 (MQ=255)
                                                                                    ataatCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCaaaa                                                           <  2:280683/148‑1 (MQ=255)
                                                                                    ataatCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCaaaa                                                           <  1:637098/148‑1 (MQ=255)
                                                                                    ataatCAAATGATCGCGATGTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCCACATCCTCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGTCTTGATCGGTCAGCaaaa                                                           <  1:637051/148‑1 (MQ=255)
                                                                                     taatCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAat                                                          <  1:3107692/148‑1 (MQ=255)
                                                                                      aatCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAata                                                         >  1:2693928/1‑148 (MQ=255)
                                                                                       atCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAatat                                                        >  2:568717/1‑148 (MQ=255)
                                                                                         cAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTg                                                      >  1:2348199/1‑148 (MQ=255)
                                                                                         cAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTg                                                      <  2:13436/148‑1 (MQ=255)
                                                                                          aaaTGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGc                                                     <  2:2465534/148‑1 (MQ=255)
                                                                                                   cgATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCACAATATTGCCGACCCCTa                                            >  2:2380250/1‑148 (MQ=255)
                                                                                                   cgATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTa                                            <  1:593796/148‑1 (MQ=255)
                                                                                                   cgATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTa                                            >  2:2380174/1‑148 (MQ=255)
                                                                                                     aTTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAg                                          >  2:2126869/1‑148 (MQ=255)
                                                                                                     aTTGGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATA‑TGCCGACCCCTAAGa                                         <  1:2912818/148‑1 (MQ=255)
                                                                                                      tttGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGa                                         >  2:2126577/1‑148 (MQ=255)
                                                                                                      tttGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGa                                         <  2:2462232/148‑1 (MQ=255)
                                                                                                       ttGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGAc                                        >  1:3167246/1‑148 (MQ=255)
                                                                                                         gCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTa                                      <  2:293054/148‑1 (MQ=255)
                                                                                                           cATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTaaa                                    <  1:2808895/148‑1 (MQ=255)
                                                                                                              gTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATa                                 <  1:3311341/148‑1 (MQ=255)
                                                                                                              gTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATAGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATa                                 >  1:1480986/1‑148 (MQ=255)
                                                                                                                cGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACg                               >  2:1528828/1‑148 (MQ=255)
                                                                                                                cGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGACGACCCCTAAGACTAAAATACg                               >  1:3360641/1‑148 (MQ=255)
                                                                                                                  ccAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCa                             <  2:2867659/148‑1 (MQ=255)
                                                                                                                   cAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCAt                            <  1:1019872/148‑1 (MQ=255)
                                                                                                                   cAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCAt                            <  1:2260015/148‑1 (MQ=255)
                                                                                                                    aaGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCAtt                           <  1:3483785/148‑1 (MQ=255)
                                                                                                                     agcagcTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTa                          >  1:707409/1‑148 (MQ=255)
                                                                                                                     agcagcTCCAGTCCCTCCGTGCCGCCATAGATGATCTCTACATAATCCGGCAGTATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATTGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTa                          <  2:2148474/148‑1 (MQ=255)
                                                                                                                      gcagcTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTAc                         >  1:185406/1‑148 (MQ=255)
                                                                                                                      gcagcTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTAc                         >  1:1698453/1‑148 (MQ=255)
                                                                                                                      gcagcTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTAc                         >  2:1862269/1‑148 (MQ=255)
                                                                                                                         gcTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTACGACTAAAATACGCATTACAGa                      >  1:2169765/1‑148 (MQ=255)
                                                                                                                         gcTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGa                      >  1:1752991/1‑148 (MQ=255)
                                                                                                                          cTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGaa                     >  2:2169818/1‑148 (MQ=255)
                                                                                                                          cTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGaa                     >  2:391725/1‑148 (MQ=255)
                                                                                                                          cTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGaa                     <  1:1209751/148‑1 (MQ=255)
                                                                                                                            ccATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAAcc                   <  1:313461/148‑1 (MQ=255)
                                                                                                                             cATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCt                  <  2:3146041/148‑1 (MQ=255)
                                                                                                                              aTTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCtt                 >  1:1239425/1‑148 (MQ=255)
                                                                                                                                            gccATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACtt   <  2:2007111/148‑1 (MQ=255)
                                                                                                                                             ccATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTc  >  2:2396582/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
TTGGTAAACAACGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTC  >  CP009273/3134383‑3134671

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTGTTGGTAAACAACGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCC  >  CP009273/3134380‑3134677
                                                                                                                                                    |                                                                                                                                                     
TTGTTGGTAAACAACGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAG                                                                                                                                                      <  SRR19973066.1388233/150‑1 (MQ=60)
 TGTTGGTAAACAACGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGA                                                                                                                                                     >  SRR19973066.1377954/1‑150 (MQ=60)
      GTAAACAACGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATC                                                                                                                                                >  SRR19973066.318712/1‑150 (MQ=60)
      GTAAACAACGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATC                                                                                                                                                <  SRR19973066.613903/150‑1 (MQ=60)
      GTAAACAACGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATC                                                                                                                                                <  SRR19973066.613491/150‑1 (MQ=60)
      GTAAACAACGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATC                                                                                                                                                <  SRR19973066.3583382/150‑1 (MQ=60)
       TAAACAACGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCT                                                                                                                                               <  SRR19973066.1844306/150‑1 (MQ=60)
         AACAACGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCA                                                                                                                                             >  SRR19973066.1337695/1‑150 (MQ=60)
            AACGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACA                                                                                                                                          >  SRR19973066.2886897/1‑150 (MQ=60)
                CCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAAT                                                                                                                                      <  SRR19973066.1827503/150‑1 (MQ=60)
                CCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAAT                                                                                                                                      <  SRR19973066.1280571/150‑1 (MQ=60)
                       TTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAG                                                                                                                               >  SRR19973066.2729051/1‑150 (MQ=60)
                          TTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAAT                                                                                                                            <  SRR19973066.156767/150‑1 (MQ=60)
                          TTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAAT                                                                                                                            <  SRR19973066.2939395/150‑1 (MQ=60)
                          TTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAAT                                                                                                                            <  SRR19973066.1222957/150‑1 (MQ=60)
                           TCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATG                                                                                                                           <  SRR19973066.47164/150‑1 (MQ=60)
                            CATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGT                                                                                                                          >  SRR19973066.708717/1‑150 (MQ=60)
                             ATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTA                                                                                                                         <  SRR19973066.806486/150‑1 (MQ=60)
                                     GGATCATCATCGTTCCCGGCGCGTTCTTTCTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCT                                                                                                                 <  SRR19973066.1401154/150‑1 (MQ=60)
                                       ATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCT                                                                                                               >  SRR19973066.2405822/1‑150 (MQ=60)
                                       ATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCT                                                                                                               >  SRR19973066.2405659/1‑150 (MQ=60)
                                       ATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCT                                                                                                               <  SRR19973066.1830887/150‑1 (MQ=60)
                                       ATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCT                                                                                                               <  SRR19973066.932812/150‑1 (MQ=60)
                                        TCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTA                                                                                                              >  SRR19973066.3141975/1‑150 (MQ=60)
                                           TCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACG                                                                                                           >  SRR19973066.1072202/1‑150 (MQ=60)
                                             ATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCT                                                                                                         <  SRR19973066.2989177/150‑1 (MQ=60)
                                             ATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCT                                                                                                         >  SRR19973066.3446586/1‑150 (MQ=60)
                                             ATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCAGAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCT                                                                                                         >  SRR19973066.3447321/1‑150 (MQ=60)
                                               CGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATACGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCCCCGTGCCGCCCTAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTC                                                                                                       >  SRR19973066.1062718/1‑150 (MQ=60)
                                                GTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCG                                                                                                      <  SRR19973066.175669/150‑1 (MQ=60)
                                                 TTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGA                                                                                                     <  SRR19973066.3064664/150‑1 (MQ=60)
                                                   CCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATGTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACA                                                                                                   <  SRR19973066.1163997/150‑1 (MQ=60)
                                                     CGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAAT                                                                                                 <  SRR19973066.1315264/150‑1 (MQ=60)
                                                      GGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATC                                                                                                <  SRR19973066.3279085/150‑1 (MQ=60)
                                                          CGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCA                                                                                            >  SRR19973066.3428717/1‑150 (MQ=60)
                                                           GTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCAC                                                                                           >  SRR19973066.2034316/1‑150 (MQ=60)
                                                           GTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCAC                                                                                           >  SRR19973066.3523380/1‑150 (MQ=60)
                                                           GTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCAC                                                                                           <  SRR19973066.63602/150‑1 (MQ=60)
                                                                  TTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATG                                                                                    <  SRR19973066.2714922/150‑1 (MQ=60)
                                                                  ATCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATGTGCCAGGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATG                                                                                    <  SRR19973066.2714895/150‑1 (MQ=60)
                                                                    CGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATGTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGC                                                                                  <  SRR19973066.3430358/150‑1 (MQ=60)
                                                                    CGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCACCATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGC                                                                                  <  SRR19973066.3431083/150‑1 (MQ=60)
                                                                     GAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCT                                                                                 >  SRR19973066.2230909/1‑150 (MQ=60)
                                                                            TGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGG                                                                          >  SRR19973066.508344/1‑150 (MQ=60)
                                                                            TGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGG                                                                          >  SRR19973066.927659/1‑150 (MQ=60)
                                                                                ATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAG                                                                      >  SRR19973066.1657755/1‑150 (MQ=60)
                                                                                   CGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAA                                                                   >  SRR19973066.1454732/1‑150 (MQ=60)
                                                                                   CGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAA                                                                   >  SRR19973066.3238337/1‑150 (MQ=60)
                                                                                    GCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAA                                                                  <  SRR19973066.61146/150‑1 (MQ=60)
                                                                                      AATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAAT                                                                <  SRR19973066.2944266/150‑1 (MQ=60)
                                                                                       ATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATA                                                               <  SRR19973066.641880/150‑1 (MQ=60)
                                                                                       ATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATA                                                               <  SRR19973066.641691/150‑1 (MQ=60)
                                                                                       ATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATA                                                               <  SRR19973066.641677/150‑1 (MQ=60)
                                                                                       ATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATA                                                               >  SRR19973066.2711927/1‑150 (MQ=60)
                                                                                       ATAATCAAATGATCGCGATGTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCCACATCCTCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGTCTTGATCGGTCAGCAAAATA                                                               <  SRR19973066.641644/150‑1 (MQ=60)
                                                                                        TAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATAT                                                              <  SRR19973066.3128446/150‑1 (MQ=60)
                                                                                          ATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTG                                                            >  SRR19973066.2364164/1‑150 (MQ=60)
                                                                                           TCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGC                                                           >  SRR19973066.511301/1‑150 (MQ=60)
                                                                                                      CGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAG                                                <  SRR19973066.597908/150‑1 (MQ=60)
                                                                                                       GATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGA                                               <  SRR19973066.1680000/150‑1 (MQ=60)
                                                                                                        ATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGAC                                              >  SRR19973066.3188341/1‑150 (MQ=60)
                                                                                                        ATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGAC                                              <  SRR19973066.3524128/150‑1 (MQ=60)
                                                                                                        ATTGGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATA‑TGCCGACCCCTAAGACT                                             <  SRR19973066.2932254/150‑1 (MQ=60)
                                                                                                              CATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAAT                                        <  SRR19973066.1495804/150‑1 (MQ=60)
                                                                                                              CATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAAT                                        <  SRR19973066.2827741/150‑1 (MQ=60)
                                                                                                              CATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAAT                                        <  SRR19973066.2827794/150‑1 (MQ=60)
                                                                                                               ATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATAGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATA                                       >  SRR19973066.1491393/1‑150 (MQ=60)
                                                                                                                 GTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACG                                     <  SRR19973066.3333309/150‑1 (MQ=60)
                                                                                                                 GTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACG                                     <  SRR19973066.2097403/150‑1 (MQ=60)
                                                                                                                 GTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGACGACCCCTAAGACTAAAATACG                                     >  SRR19973066.3383048/1‑150 (MQ=60)
                                                                                                                      CAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTA                                >  SRR19973066.712645/1‑150 (MQ=60)
                                                                                                                      CAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTA                                <  SRR19973066.1027375/150‑1 (MQ=60)
                                                                                                                      CAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTA                                <  SRR19973066.2275582/150‑1 (MQ=60)
                                                                                                                       AAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTAC                               <  SRR19973066.3507026/150‑1 (MQ=60)
                                                                                                                       AAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTAC                               >  SRR19973066.187020/1‑150 (MQ=60)
                                                                                                                       AAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTAC                               <  SRR19973066.1924759/150‑1 (MQ=60)
                                                                                                                       AAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTAC                               >  SRR19973066.1710796/1‑150 (MQ=60)
                                                                                                                        AGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACA                              <  SRR19973066.716721/150‑1 (MQ=60)
                                                                                                                          CAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTACGACTAAAATACGCATTACAGA                            >  SRR19973066.2184831/1‑150 (MQ=60)
                                                                                                                          CAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGA                            >  SRR19973066.1765597/1‑150 (MQ=60)
                                                                                                                          CAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGA                            >  SRR19973066.1585355/1‑150 (MQ=60)
                                                                                                                          CAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGA                            >  SRR19973066.1765547/1‑150 (MQ=60)
                                                                                                                            GCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAAC                          <  SRR19973066.927528/150‑1 (MQ=60)
                                                                                                                            GCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAAC                          <  SRR19973066.1295889/150‑1 (MQ=60)
                                                                                                                            GCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAAC                          <  SRR19973066.2400016/150‑1 (MQ=60)
                                                                                                                            GCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAAC                          <  SRR19973066.1806326/150‑1 (MQ=60)
                                                                                                                             CTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTCAAATACGCATTACAGAACC                         >  SRR19973066.3340328/1‑150 (MQ=60)
                                                                                                                             CTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACC                         <  SRR19973066.1218213/150‑1 (MQ=60)
                                                                                                                             CTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACC                         <  SRR19973066.1218204/150‑1 (MQ=60)
                                                                                                                              TCCATTCCTGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCT                        >  SRR19973066.3490519/1‑150 (MQ=60)
                                                                                                                               CCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTT                       >  SRR19973066.1248268/1‑150 (MQ=60)
                                                                                                                               CCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTT                       <  SRR19973066.315776/150‑1 (MQ=60)
                                                                                                                               CCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTT                       <  SRR19973066.3277086/150‑1 (MQ=60)
                                                                                                                                     CCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGA                 >  SRR19973066.3293342/1‑150 (MQ=60)
                                                                                                                                                  ATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTG    >  SRR19973066.464180/1‑150 (MQ=60)
                                                                                                                                                  ATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTG    >  SRR19973066.1625679/1‑150 (MQ=60)
                                                                                                                                                    AGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCC  >  SRR19973066.1836862/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
TTGTTGGTAAACAACGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCGTTGCC  >  CP009273/3134380‑3134677

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑