Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,525,950 C→T 8.5% intergenic (‑152/‑227) yhgE ← / → pck DUF4153 family putative inner membrane protein/phosphoenolpyruvate carboxykinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,525,9500CT 8.5% 761.6 / 40.7 223intergenic (‑152/‑227)yhgE/pckDUF4153 family putative inner membrane protein/phosphoenolpyruvate carboxykinase
Reads supporting (aligned to +/- strand):  ref base C (101/103);  new base T (11/8);  total (112/111)
Fisher's exact test for biased strand distribution p-value = 6.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

Only 100 of 225 total aligned reads displayed.

TTATCCAGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATC‑AAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGTTTTTTGTCAAATATGAATTTCTCCAGATACGTAAA  >  CP009273/3525804‑3526097
                                                                                                                                                   |                                                                                                                                                   
ttATCCAGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATC‑AAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACt                                                                                                                                                    >  2:2938230/1‑148 (MQ=255)
ttATCCAGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATC‑AAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACt                                                                                                                                                    >  1:230206/1‑148 (MQ=255)
   tCCAGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATC‑AAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTcac                                                                                                                                                 >  2:2090858/1‑148 (MQ=255)
    ccAGAATCAAGAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATC‑AAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTcaca                                                                                                                                                >  1:466539/1‑148 (MQ=255)
    ccAGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATC‑AAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTcaca                                                                                                                                                <  2:1628509/148‑1 (MQ=255)
    ccAGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATC‑AAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTcaca                                                                                                                                                >  1:2143257/1‑148 (MQ=255)
      aGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATC‑AAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAat                                                                                                                                              >  1:2549960/1‑148 (MQ=255)
           cAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATC‑AAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCATTCACAATATTcc                                                                                                                                         >  1:992678/1‑148 (MQ=255)
           cAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATC‑AAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTcc                                                                                                                                         <  2:3237202/148‑1 (MQ=255)
              aaGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATC‑AAAAAAAGTTAGCGTGGTGAATCGATGCTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGc                                                                                                                                      >  2:1008682/1‑148 (MQ=255)
                ggTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATC‑AAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCa                                                                                                                                    <  1:1114342/148‑1 (MQ=255)
                ggTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATC‑AAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCa                                                                                                                                    <  2:267853/148‑1 (MQ=255)
                   gggTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATC‑AAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCatat                                                                                                                                 <  1:2288650/148‑1 (MQ=255)
                     gTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATC‑AAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATaa                                                                                                                               <  1:2921486/148‑1 (MQ=255)
                      ttAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATC‑AAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATaaa                                                                                                                              <  1:2759907/148‑1 (MQ=255)
                       tAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAAAAAAGTAAGGTTGGTGAAAGTTAACTTTACCGGTTAATTTTGAAAAAATTTCATTATGCAATCCGATACCATAAAAAATATACCCCCAAAATaaa                                                                                                                              >  2:348545/1‑148 (MQ=255)
                       tAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATC‑AAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAAc                                                                                                                             >  2:2492425/1‑148 (MQ=255)
                             tCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATC‑AAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGAt                                                                                                                       <  2:1184781/148‑1 (MQ=255)
                             tCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATC‑AAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGAt                                                                                                                       <  1:1626207/148‑1 (MQ=255)
                                   ccGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATC‑AAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAAcc                                                                                                                 <  1:2029229/148‑1 (MQ=255)
                                   ccGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATC‑AAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAAcc                                                                                                                 <  2:1728162/148‑1 (MQ=255)
                                      ggCAGTAGTATTTTGCTTTTTTCAGAAAATAATC‑AAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCttt                                                                                                              <  1:597184/148‑1 (MQ=255)
                                      ggCAGTAGTATTTTGCTTTTTTCAGAAAATAATC‑AAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCttt                                                                                                              <  1:3040843/148‑1 (MQ=255)
                                      ggCAGTAGTATTTTGCTATTTTCAGAAAATAATC‑AAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCATTCACAATATTCCCGCCATATAAACCAAGATTTAACCttt                                                                                                              <  2:2781144/148‑1 (MQ=255)
                                       gCAGTAGTATTTTGCTTTTTTCAGAAAATAATC‑AAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCtttt                                                                                                             <  2:715753/148‑1 (MQ=255)
                                         agtagtATTTTGCTTTTTACAGAAAATAATC‑AAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATAGTCCCGCCATATAAACCAAGATTTAACCTTTTga                                                                                                           <  2:870077/148‑1 (MQ=255)
                                            agtaTTTTGCTTTTTTCAGAAAATAATC‑AAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGaa                                                                                                        >  2:2562206/1‑148 (MQ=255)
                                            agtaTTTTGCTTTTTTCAGAAAATAATC‑AAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATCCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGaa                                                                                                        <  2:3108693/148‑1 (MQ=255)
                                              taTTTTGCTTTTTTCAGAAAATAATC‑AAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACa                                                                                                      <  2:1402963/148‑1 (MQ=255)
                                              taTTTTGCTTTTTTCAGAAAATAATC‑AAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACa                                                                                                      >  1:847111/1‑148 (MQ=255)
                                               aTTTTGCTTTTTTCAGAAAATAATC‑AAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACAt                                                                                                     >  2:2356219/1‑148 (MQ=255)
                                               aTTTTGCTTTTTTCAGAAAATAATC‑AAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACAt                                                                                                     <  2:2930671/148‑1 (MQ=255)
                                                ttttGCTTTTTTCAGAAAATAATC‑AAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACAtt                                                                                                    <  1:2837497/148‑1 (MQ=255)
                                                   tGCTATTTTCAGAAAATAATC‑AAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTc                                                                                                 <  2:43615/148‑1 (MQ=255)
                                                    gCTTTTTTCAGAAAATAATC‑AAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTcc                                                                                                >  2:3034428/1‑148 (MQ=255)
                                                    gCTTTTTTCAGAAAATAATC‑AAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTcc                                                                                                >  1:780379/1‑148 (MQ=255)
                                                       tttttCAGAAAATAATC‑AAAAAAAGTTAGAGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCcaca                                                                                             <  2:8488/148‑1 (MQ=255)
                                                       ttttaCAGAAAATAATC‑ACAAAAAGTTAGAGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCcaca                                                                                             <  2:1910385/148‑1 (MQ=255)
                                                             aGAAAATAATC‑AAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTaaa                                                                                       <  2:1837263/148‑1 (MQ=255)
                                                               aaaaTAATC‑AAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAATATTTAACCTTTTGAGAACATTTTCCACACCTAAAAt                                                                                     >  1:183245/1‑148 (MQ=255)
                                                               aaaaTAATC‑AAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAAt                                                                                     >  1:2742915/1‑148 (MQ=255)
                                                               aaaaTAATC‑AAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAAt                                                                                     >  1:149920/1‑148 (MQ=255)
                                                               aaaaTAATC‑AAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAAt                                                                                     >  2:300000/1‑148 (MQ=255)
                                                               aaaaTAATC‑AAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACGCACAATATTCCCGCCATATAAACCAAGATTTAAGCTTGTGAGAACAGTTTCCACACCGCAAAt                                                                                     >  2:2063568/1‑148 (MQ=255)
                                                                aaaTAATC‑AAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATg                                                                                    <  2:2870550/148‑1 (MQ=255)
                                                                 aataatC‑AAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCATTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGc                                                                                   <  2:2436137/148‑1 (MQ=255)
                                                                 aataatC‑AAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGc                                                                                   >  1:398497/1‑148 (MQ=255)
                                                                 aataatC‑AAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATCCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGc                                                                                   >  1:1076186/1‑148 (MQ=255)
                                                                   taatC‑AAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTa                                                                                 <  2:1307026/148‑1 (MQ=255)
                                                                    aatC‑AAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTAt                                                                                >  1:1493598/1‑148 (MQ=255)
                                                                    aatC‑AAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTAt                                                                                <  2:1672733/148‑1 (MQ=255)
                                                                     atC‑AAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTAtt                                                                               >  1:1992760/1‑148 (MQ=255)
                                                                     atC‑AAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTAtt                                                                               >  1:2819796/1‑148 (MQ=255)
                                                                     atC‑AAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATATTCCACACCTAAAATGCTAtt                                                                               >  1:862730/1‑148 (MQ=255)
                                                                      tC‑AAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTAttt                                                                              <  1:937667/148‑1 (MQ=255)
                                                                          aaaaaaGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTg                                                                           <  2:1750287/148‑1 (MQ=255)
                                                                          aaaaaaGTGAGCGTGTTGAATAGATACTGTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATACTCCTTGATTTTACCTTTTGAGAACATTTTCCCCACCTAAAATGCTATTTCTg                                                                           <  2:763512/148‑1 (MQ=255)
                                                                           aaaaaGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGc                                                                          >  2:1545034/1‑148 (MQ=255)
                                                                              aaGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGat                                                                       <  2:2828059/148‑1 (MQ=255)
                                                                              aaGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTATGCGat                                                                       >  2:1192006/1‑148 (MQ=255)
                                                                               aGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGata                                                                      <  1:3173610/148‑1 (MQ=255)
                                                                                gTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGataa                                                                     <  2:1973285/148‑1 (MQ=255)
                                                                                  tAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGataata                                                                   >  2:2099674/1‑148 (MQ=255)
                                                                                         gtgAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCg                                                            >  1:2786507/1‑148 (MQ=255)
                                                                                          tgAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCATTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGt                                                           <  1:1184305/148‑1 (MQ=255)
                                                                                             aTCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATACTAGCAACCGTTTc                                                        >  1:2498288/1‑148 (MQ=255)
                                                                                             aTCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTc                                                        >  2:679923/1‑148 (MQ=255)
                                                                                                gATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTg                                                     <  1:2848222/148‑1 (MQ=255)
                                                                                                gATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTg                                                     <  2:766729/148‑1 (MQ=255)
                                                                                                 aTACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGa                                                    <  1:2621673/148‑1 (MQ=255)
                                                                                                 aTACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTATCGTGa                                                    >  1:2647399/1‑148 (MQ=255)
                                                                                                   aCTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACa                                                  >  2:3304335/1‑148 (MQ=255)
                                                                                                    cTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAg                                                 >  2:657007/1‑148 (MQ=255)
                                                                                                        aCCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAAt                                             <  1:2742207/148‑1 (MQ=255)
                                                                                                            gTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCATTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACg                                         >  1:1637890/1‑148 (MQ=255)
                                                                                                            gTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACg                                         >  2:1292404/1‑148 (MQ=255)
                                                                                                             ttGAATTTGCATCAATTTCATTCAGGAATGCGATTCCATTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACgg                                        <  2:3176403/148‑1 (MQ=255)
                                                                                                               gAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAg                                      <  2:1177672/148‑1 (MQ=255)
                                                                                                                aaTTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGt                                     <  1:1757182/148‑1 (MQ=255)
                                                                                                                   ttGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGtttt                                  <  1:3223693/148‑1 (MQ=255)
                                                                                                                       aTCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGTTTTTTGt                              <  1:2962699/148‑1 (MQ=255)
                                                                                                                        tCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGTTTTTTGTc                             >  1:1274775/1‑148 (MQ=255)
                                                                                                                        tCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGTTTTTTGTc                             >  2:1800265/1‑148 (MQ=255)
                                                                                                                          aaTTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGTTTTTTGTCaa                           >  1:754297/1‑148 (MQ=255)
                                                                                                                            tttCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGTTTTTTGTCAAat                         <  2:2143257/148‑1 (MQ=255)
                                                                                                                             ttcattcaGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGTTTTTTGTCAAata                        >  1:2975175/1‑148 (MQ=255)
                                                                                                                               cattcaGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGTTTTTTGTCAAATATg                      >  1:876845/1‑148 (MQ=255)
                                                                                                                               cattcaGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGTTTTTTGTCAAATATg                      <  2:121009/148‑1 (MQ=255)
                                                                                                                                attcaGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGTTTTTTGTCAAATATGa                     <  2:1245554/148‑1 (MQ=255)
                                                                                                                                 ttcaGGAATGCGATTCTATTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGTTTTTTGTCAAATATGaa                    <  1:2257783/148‑1 (MQ=255)
                                                                                                                                             aTTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGTTTTTTGTCAAATATGAATTTCTCCAGATa        <  1:1360486/148‑1 (MQ=255)
                                                                                                                                             aTTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGTTTTTTGTCAAATATGAATTTCTCCAGATa        <  2:1533588/148‑1 (MQ=255)
                                                                                                                                              ttCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGTTTTTTGTCAAATATGAATTTCTCCAGATAc       >  1:1721049/1‑148 (MQ=255)
                                                                                                                                              ttCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGTTTTTTGTCAAATATGAATTTCTCCAGATAc       >  2:3586/1‑148 (MQ=255)
                                                                                                                                              ttCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGTTTTTTGTCAAATATGAATTTCTCCAGATAc       >  2:153489/1‑148 (MQ=255)
                                                                                                                                               tCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGTTTTTTGTCAAATATGAATTTCTCCAGATACg      >  1:112169/1‑148 (MQ=255)
                                                                                                                                                ccACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGTTTTTTGTCAAATATGAATTTCTCCAGATACGt     <  2:2549960/148‑1 (MQ=255)
                                                                                                                                                  aCTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGTTTTTTGTCAAATATGAATTTCTCCAGATACGTaa   <  2:3212757/148‑1 (MQ=255)
                                                                                                                                                  aCTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGTTTTTTGTCAAATATGAATTTCTCCAGATACGTaa   <  1:1544533/148‑1 (MQ=255)
                                                                                                                                                   cTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGTTTTTTGTCAAATATGAATTTCTCCAGATACGTaaa  <  2:295719/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TTATCCAGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATC‑AAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGTTTTTTGTCAAATATGAATTTCTCCAGATACGTAAA  >  CP009273/3525804‑3526097

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A