Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F159 I0 R1 879 305.9 9588012 96.6% 9262019 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,525,950 C→T 34.7% intergenic (‑152/‑227) yhgE ← / → pck DUF4153 family putative inner membrane protein/phosphoenolpyruvate carboxykinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,525,9500CT34.7% 207.8 / 167.5 162intergenic (‑152/‑227)yhgE/pckDUF4153 family putative inner membrane protein/phosphoenolpyruvate carboxykinase
Reads supporting (aligned to +/- strand):  ref base C (57/48);  new base T (32/24);  total (90/72)
Fisher's exact test for biased strand distribution p-value = 7.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 162 total aligned reads displayed.

GGTTATCCAGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGTTTTTTGTCAAATATGAATTTCTCCAGATACGTAA  >  CP009273/3525802‑3526096
                                                                                                                                                    |                                                                                                                                                  
ggTTATCCAGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACt                                                                                                                                                   <  1:1505928/150‑1 (MQ=255)
    aTCCAGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTcaca                                                                                                                                               >  2:4482739/1‑150 (MQ=255)
     tCCAGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCATTCACaa                                                                                                                                              <  2:646444/150‑1 (MQ=255)
     tCCAGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACaa                                                                                                                                              <  1:4613664/150‑1 (MQ=255)
     tCCAGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATATTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACaa                                                                                                                                              >  1:2071785/1‑150 (MQ=255)
        aGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAatat                                                                                                                                           <  1:1089658/150‑1 (MQ=255)
        aGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAatat                                                                                                                                           <  1:1089674/150‑1 (MQ=255)
         gAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCATTCACAATAtt                                                                                                                                          <  1:4623005/150‑1 (MQ=255)
            tCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCTGGAATGCGATTCCACTCACAATATTccc                                                                                                                                       >  1:1324957/1‑150 (MQ=255)
             cAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCATTCACAATATTCCCg                                                                                                                                      >  2:1057559/1‑150 (MQ=255)
             cAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCATTCACAATATTCCCg                                                                                                                                      >  1:2943231/1‑150 (MQ=255)
             cAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCATTCACAATATTCCCg                                                                                                                                      >  1:3641064/1‑150 (MQ=255)
              aaaaGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTTATTCAGGAATGCGATTCCACTCACAATATTCCCGc                                                                                                                                     >  1:2189689/1‑150 (MQ=255)
                aaGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCATTCACAATATTCCCGCCa                                                                                                                                   >  2:2928769/1‑150 (MQ=255)
                 aGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCat                                                                                                                                  >  2:701517/1‑150 (MQ=255)
                   gTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCatat                                                                                                                                <  2:3473553/150‑1 (MQ=255)
                   gTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCatat                                                                                                                                <  1:3642540/150‑1 (MQ=255)
                   gTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCatat                                                                                                                                <  2:2190371/150‑1 (MQ=255)
                    tGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCATTCACAATATTCCCGCCAtata                                                                                                                               <  2:2773393/150‑1 (MQ=255)
                       gTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAAc                                                                                                                            <  2:1911664/150‑1 (MQ=255)
                         tAATTATCGCATCCGGTCAGTAGTATTTTGCTTTTTTCATAAAATAATAAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCa                                                                                                                          <  2:4458368/150‑1 (MQ=255)
                           aTTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAg                                                                                                                        <  1:1793534/150‑1 (MQ=255)
                              aTCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGAtt                                                                                                                     <  2:4296951/150‑1 (MQ=255)
                               tCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGAttt                                                                                                                    <  1:123781/150‑1 (MQ=255)
                                cGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTa                                                                                                                   <  1:3007355/150‑1 (MQ=255)
                                cGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTa                                                                                                                   <  1:3007358/150‑1 (MQ=255)
                                 gCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTaa                                                                                                                  >  2:4173405/1‑150 (MQ=255)
                                 gCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTCTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTaa                                                                                                                  >  2:4172922/1‑150 (MQ=255)
                                      cGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCATTCACAATATTCCCGCCATATAAACCAAGATTTAACCttt                                                                                                             <  1:1713009/150‑1 (MQ=255)
                                       gggCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCATTCACAATATTCCCGCCATATAAACCAAGATTTAACCtttt                                                                                                            <  2:1811122/150‑1 (MQ=255)
                                       gggCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCATTCACAATATTCCCGCCATATAAACCAAGATTTAACCtttt                                                                                                            <  1:2140227/150‑1 (MQ=255)
                                        ggCAGTAGTATTTTGCTTTTTTCATAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCTGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTg                                                                                                           <  1:2848263/150‑1 (MQ=255)
                                        ggCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTg                                                                                                           <  2:788/150‑1 (MQ=255)
                                           agtagtATTTTGATTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCATTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTgaga                                                                                                        <  2:2783895/150‑1 (MQ=255)
                                             tagtaTTTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAAc                                                                                                      >  1:496441/1‑150 (MQ=255)
                                              agtaTTTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACa                                                                                                     >  2:1300425/1‑150 (MQ=255)
                                               gtaTTTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACAt                                                                                                    <  1:3544515/150‑1 (MQ=255)
                                                 aTTTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACAttt                                                                                                  >  1:2003339/1‑150 (MQ=255)
                                                 aTTTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACAttt                                                                                                  <  1:2049636/150‑1 (MQ=255)
                                                 aTTATGCTTTTAAAAGAAAATAAACAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCATTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACAttt                                                                                                  <  2:3868794/150‑1 (MQ=255)
                                                   tttGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTc                                                                                                >  1:3867703/1‑150 (MQ=255)
                                                    ttGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCATTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTcc                                                                                               >  2:2264591/1‑150 (MQ=255)
                                                    ttGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCATTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTcc                                                                                               <  2:2053/150‑1 (MQ=255)
                                                    ttGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCATTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTcc                                                                                               >  2:3647672/1‑150 (MQ=255)
                                                    ttGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTcc                                                                                               <  2:2662000/150‑1 (MQ=255)
                                                     tGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCca                                                                                              >  1:448666/1‑150 (MQ=255)
                                                     tGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCca                                                                                              >  1:800116/1‑150 (MQ=255)
                                                     tGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTGTTGAGAACATTTTCca                                                                                              >  2:4198745/1‑150 (MQ=255)
                                                       cTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCcaca                                                                                            <  2:1471135/150‑1 (MQ=255)
                                                        ttttttCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCATTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCacac                                                                                           <  1:2354974/150‑1 (MQ=255)
                                                            ttCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCATTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTaa                                                                                       <  2:4529271/150‑1 (MQ=255)
                                                              cAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTACACACCTaaaa                                                                                     >  2:4010093/1‑150 (MQ=255)
                                                               aGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCATTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAAt                                                                                    >  2:115399/1‑150 (MQ=255)
                                                               aGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAAt                                                                                    >  2:3947889/1‑150 (MQ=255)
                                                               aGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAAt                                                                                    <  1:1072357/150‑1 (MQ=255)
                                                                 aaaaTAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGc                                                                                  >  1:1016620/1‑150 (MQ=255)
                                                                   aataatCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTa                                                                                <  2:3619467/150‑1 (MQ=255)
                                                                               aaGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCATTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGataa                                                                    >  2:3142148/1‑150 (MQ=255)
                                                                               aaGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGataa                                                                    >  1:4744434/1‑150 (MQ=255)
                                                                               aaGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGataa                                                                    >  1:4661096/1‑150 (MQ=255)
                                                                                 gtgaGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCATTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGataata                                                                  <  2:3829820/147‑1 (MQ=255)
                                                                                 gTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCATTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGataata                                                                  <  2:3829788/150‑1 (MQ=255)
                                                                                 gTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCATTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGataata                                                                  <  2:484704/150‑1 (MQ=255)
                                                                                 gTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCATTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGataata                                                                  <  2:484688/150‑1 (MQ=255)
                                                                                 gTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGataata                                                                  >  2:4505906/1‑150 (MQ=255)
                                                                                    aGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCa                                                               >  1:4274344/1‑150 (MQ=255)
                                                                                        tggtgAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCATTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCg                                                           >  1:4424031/1‑150 (MQ=255)
                                                                                           tgAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCATTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGttt                                                        >  1:505624/1‑150 (MQ=255)
                                                                                             aaTCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCg                                                      >  2:735612/1‑150 (MQ=255)
                                                                                              aTCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGt                                                     >  1:3524596/1‑150 (MQ=255)
                                                                                                      tttACCGGTTGAATTTGCATCAATTTTATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGaa                                             <  2:2189689/150‑1 (MQ=255)
                                                                                                       ttACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAAt                                            >  1:1119527/1‑150 (MQ=255)
                                                                                                        tACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTAGTGACAGGAATc                                           <  2:4274344/150‑1 (MQ=255)
                                                                                                         aCCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCATTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCa                                          >  1:2537563/1‑150 (MQ=255)
                                                                                                         aCCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCATTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCa                                          >  1:2537549/1‑150 (MQ=255)
                                                                                                             gTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCATTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGa                                      >  2:820427/1‑150 (MQ=255)
                                                                                                                  aTTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGtttt                                 >  1:2407277/1‑150 (MQ=255)
                                                                                                                  aTTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGtttt                                 >  2:4431445/1‑150 (MQ=255)
                                                                                                                   tttGCATCAATTTCATTCAGGAATGCGATTCCATTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGttttt                                <  1:115399/150‑1 (MQ=255)
                                                                                                                     tGCATCAATTTCATTCAGGAATGCGATTCCATTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGTTTTTTg                              >  1:4121986/1‑150 (MQ=255)
                                                                                                                      gCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGTTTTTTGt                             >  2:3784830/1‑150 (MQ=255)
                                                                                                                        aTCAATTTCATTCAGGAATGCGATTCCATTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGTTTTTTGTCa                           >  1:4668463/1‑150 (MQ=255)
                                                                                                                           aaTTTCATTCAGGAATGCGATTCCATTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGTTTTTTGTCAAat                        >  2:934973/1‑150 (MQ=255)
                                                                                                                             tttCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGTTTTTTGTCAAatat                      <  2:1016620/150‑1 (MQ=255)
                                                                                                                             tttCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGTTTTTTGTCAAatat                      <  2:1324957/150‑1 (MQ=255)
                                                                                                                              ttcattcaGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGTTTTTTGTCAAATATg                     >  2:4694087/1‑150 (MQ=255)
                                                                                                                               tcattcaGGAATGCGATTCCATTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGTTTTTTGTCAAATATGa                    >  1:4475829/1‑150 (MQ=255)
                                                                                                                               tcattcaGGAATGCGATTCCATTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGTTTTTTGTCAAATATGa                    >  1:1650944/1‑150 (MQ=255)
                                                                                                                                 attcaGGAATGCGATTCCATTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGTTTTTTGTCAAATATGAAt                  <  1:1575994/150‑1 (MQ=255)
                                                                                                                                   tcaGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGTTTTTTGTCAAATATGAAttt                <  2:113443/150‑1 (MQ=255)
                                                                                                                                    caGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGTTTTTTGTCAAATATGAATTtc               <  2:565549/150‑1 (MQ=255)
                                                                                                                                    caGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGTTTTTTGTCAAATATGAATTtc               >  1:1783333/1‑150 (MQ=255)
                                                                                                                                     aGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGTTTTTTGTCAAATATGAATTtct              >  1:1977877/1‑150 (MQ=255)
                                                                                                                                          tGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGTTTTTTGTCAAATATGAATTTCTCCAGa         >  2:2582104/1‑150 (MQ=255)
                                                                                                                                           gCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGTTTTTTGTCAAATATGAATTTCTCCAGAt        <  1:2588988/150‑1 (MQ=255)
                                                                                                                                            cGATTCCATTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGTTTTTTGTCAAATATGAATTTCTCCAGATa       >  1:3552752/1‑150 (MQ=255)
                                                                                                                                            cGATTCCATTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGTTTTTTGTCAAATATGAATTTCTCCAGATa       >  1:1620832/1‑150 (MQ=255)
                                                                                                                                             gATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGTTTTTTGTCAAATATGAATTTCTCCAGATAc      <  1:4482739/150‑1 (MQ=255)
                                                                                                                                               ttCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGTTTTTTGTCAAATATGAATTTCTCCAGATACGt    >  2:974837/1‑150 (MQ=255)
                                                                                                                                               ttCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACAGTTTCGTGACAGGAATCACGGAGTTTTTTGTCAAATATGAATTTCTCCAGATACGt    >  2:974963/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
GGTTATCCAGAATCAAAAGGTGGGTTAATTATCGCATCCGGGCAGTAGTATTTTGCTTTTTTCAGAAAATAATCAAAAAAAGTTAGCGTGGTGAATCGATACTTTACCGGTTGAATTTGCATCAATTTCATTCAGGAATGCGATTCCACTCACAATATTCCCGCCATATAAACCAAGATTTAACCTTTTGAGAACATTTTCCACACCTAAAATGCTATTTCTGCGATAATAGCAACCGTTTCGTGACAGGAATCACGGAGTTTTTTGTCAAATATGAATTTCTCCAGATACGTAA  >  CP009273/3525802‑3526096

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A