Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F309 I0 R1 1697 197.2 6627756 95.7% 6342762 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,543,959 G→T 100% A625E (GCG→GAG)  malP ← maltodextrin phosphorylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,543,9590GT99.4% 492.0 / ‑5.8 164A625E (GCG→GAG) malPmaltodextrin phosphorylase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  major base T (80/81);  minor base A (2/0);  total (83/81)
Fisher's exact test for biased strand distribution p-value = 4.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 174 total aligned reads displayed.

ATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCGCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCC  >  CP009273/3543814‑3544101
                                                                                                                                                 |                                                                                                                                              
atTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGNGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATccgcctcc                                                                                                                                              >  1:1676637/1‑148 (MQ=255)
atTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATccgcctcc                                                                                                                                              >  1:1103366/1‑148 (MQ=255)
atTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATccgcctcc                                                                                                                                              >  1:1580486/1‑148 (MQ=255)
atTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATacgcctcc                                                                                                                                              >  1:2083347/1‑148 (MQ=255)
 ttttCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATccgcctccg                                                                                                                                             >  2:294391/1‑148 (MQ=255)
 ttttCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATccgcctccg                                                                                                                                             <  2:1102088/148‑1 (MQ=255)
    tcttcACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGa                                                                                                                                          >  1:2539653/1‑148 (MQ=255)
    tcttcACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGa                                                                                                                                          >  1:2539662/1‑148 (MQ=255)
      ttcACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATc                                                                                                                                        >  2:3104823/1‑148 (MQ=255)
      ttcACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATc                                                                                                                                        >  2:3104798/1‑148 (MQ=255)
       tcACCGACTTTCTCGGCGATTTCATCGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCATTACCTGCAGTAGAAATTTGTACGGAGATAACCGCCTCCGGGATCa                                                                                                                                       >  1:819392/1‑148 (MQ=255)
       tcACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTTCCTGCAGTCGAAATTTGTTCGGAGATATACGCCTCCGGGATCa                                                                                                                                       >  1:844716/1‑148 (MQ=255)
       tcACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGCAATTTGTTCGGAGATATCCGCCTCCGGGATCa                                                                                                                                       >  2:1546776/1‑148 (MQ=255)
       tcACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGAACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTATTTACCTGCATTCGAAATTTGTTCGGAGATATACGCCTCCGGGATCa                                                                                                                                       >  2:850064/1‑148 (MQ=255)
            gACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGtttt                                                                                                                                  <  1:40874/148‑1 (MQ=255)
            gACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGtttt                                                                                                                                  <  1:446894/148‑1 (MQ=255)
             aCTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGttttt                                                                                                                                 <  1:754537/148‑1 (MQ=255)
             aCTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGttttt                                                                                                                                 >  2:659610/1‑148 (MQ=255)
             aCTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGttttt                                                                                                                                 >  1:785872/1‑148 (MQ=255)
             aCTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGttttt                                                                                                                                 >  1:785823/1‑148 (MQ=255)
                 tctcGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTccgc                                                                                                                             <  2:1469358/148‑1 (MQ=255)
                 tctcGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTccgc                                                                                                                             <  1:1369629/148‑1 (MQ=255)
                    cGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTTCCTGCAGTCGAAATTTTTTCGGAGATATACGCCGCCGGGATCAGTTTTTacgccgc                                                                                                                          >  1:2780638/1‑148 (MQ=255)
                     ggCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTccgccgcc                                                                                                                         >  1:2859321/1‑148 (MQ=255)
                     ggCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATACGCCTCCGGGATCAGTTTTTccgccgcc                                                                                                                         >  1:596015/1‑148 (MQ=255)
                        gATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGaa                                                                                                                      <  1:688366/148‑1 (MQ=255)
                        gATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGaa                                                                                                                      <  1:1007064/148‑1 (MQ=255)
                        gATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGaa                                                                                                                      <  1:2349621/148‑1 (MQ=255)
                          tttCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAAc                                                                                                                    >  2:1013051/1‑148 (MQ=255)
                          tttCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAAc                                                                                                                    >  2:1190060/1‑148 (MQ=255)
                          tttCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTAGGACATATCCGCCTCCGGGATCAGTTTTTCCCCCGCCGaaaa                                                                                                                    >  1:826948/1‑147 (MQ=255)
                           ttCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGCTCAGTTTTTCCGCCGCCGAAACg                                                                                                                   >  1:678345/1‑148 (MQ=255)
                           ttCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACg                                                                                                                   >  1:1301723/1‑148 (MQ=255)
                             cAACGTTCGCCCCATCCAGAGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCa                                                                                                                 <  1:2101086/148‑1 (MQ=255)
                              aaCGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCaa                                                                                                                <  2:560800/148‑1 (MQ=255)
                               aCGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTATTTACCTGCAGTCGAAATTTGTTCGGAGATATACGCCTCCGGGGTCAGTTTTTACGCCGCCGCAACGCaat                                                                                                               >  1:319961/1‑148 (MQ=255)
                                  ttCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCaataat                                                                                                            <  1:1085144/148‑1 (MQ=255)
                                   tCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCACTACTc                                                                                                           >  2:2858179/1‑148 (MQ=255)
                                   tCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTAGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCACTAATc                                                                                                           >  2:2771864/1‑148 (MQ=255)
                                    cGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATcc                                                                                                          <  2:2544296/148‑1 (MQ=255)
                                        ccATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCa                                                                                                      >  2:2993622/1‑148 (MQ=255)
                                         cATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAg                                                                                                     <  2:1442625/148‑1 (MQ=255)
                                          aTCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAgg                                                                                                    >  1:2975442/1‑148 (MQ=255)
                                           tCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCACTCGAAATTTGATGGGAGATATCCGACTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAAAACGGCAGGa                                                                                                   >  2:2057997/1‑148 (MQ=255)
                                            ccAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGaa                                                                                                  >  2:1380281/1‑148 (MQ=255)
                                             cAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAac                                                                                                 <  2:1690675/148‑1 (MQ=255)
                                              aGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAaca                                                                                                <  1:1387168/148‑1 (MQ=255)
                                                  tACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAAcaccac                                                                                            >  2:1782327/1‑148 (MQ=255)
                                                  tACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAAcaccac                                                                                            <  1:1272827/148‑1 (MQ=255)
                                                   aCCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACaccacc                                                                                           <  1:887209/148‑1 (MQ=255)
                                                    ccGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCt                                                                                          >  1:924201/1‑148 (MQ=255)
                                                        cAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCaa                                                                                      <  2:793005/148‑1 (MQ=255)
                                                        cAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCGTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCACCGGGATCAGTTTTTCCGCCGCCGCAACGCAATAATCCGGCAGGAACACCACCATCaa                                                                                      >  1:22776/1‑148 (MQ=255)
                                                           tAAGCGCACCATTGAGCGCCAGTTTCATATTGACGGTACCGGAAGCTTCTTTAACTGCAGTCGAAATTTTTTCGGAGATATACGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAAAAATACAGCAGGAACAAAACCTTCAAcat                                                                                   >  1:2064764/1‑146 (MQ=255)
                                                              gcgcACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATc                                                                                >  1:3017170/1‑148 (MQ=255)
                                                              gcgcACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATc                                                                                >  2:1871727/1‑148 (MQ=255)
                                                                       tGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAg                                                                       <  2:1581220/148‑1 (MQ=255)
                                                                       tGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAg                                                                       <  1:1013051/148‑1 (MQ=255)
                                                                           cgcCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGa                                                                   <  2:1314807/148‑1 (MQ=255)
                                                                                 tttCATAGTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCgttg                                                             <  2:2855978/148‑1 (MQ=255)
                                                                                     atttttccGGTACCGGAAGCTTCTTTACCTTCAGTCGAAATTTGTTCGGAGATTTCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAAATTATCGCCAACCAGCGGATCGTTGTTGa                                                         <  1:1481270/142‑1 (MQ=255)
                                                                                      tatTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGAt                                                        <  2:2260090/148‑1 (MQ=255)
                                                                                       atTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATc                                                       >  2:1536496/1‑148 (MQ=255)
                                                                                         tGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGCACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCAc                                                     >  2:2230776/1‑148 (MQ=255)
                                                                                          gCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACg                                                    <  1:2747099/148‑1 (MQ=255)
                                                                                                   ggAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACt                                           >  2:3195992/1‑148 (MQ=255)
                                                                                                      aGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTttg                                        <  2:603105/148‑1 (MQ=255)
                                                                                                       gCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTttgt                                       <  2:821867/148‑1 (MQ=255)
                                                                                                       gCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTttgt                                       <  1:1931196/148‑1 (MQ=255)
                                                                                                       gCTTCTTGACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTttgt                                       <  2:2072883/148‑1 (MQ=255)
                                                                                                       gCTTCTGTACGTGCAGTCGAAATTTGTTCGGAGTTATCAGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACGTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTttgt                                       <  2:819392/148‑1 (MQ=255)
                                                                                                          tcttTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGa                                    <  2:1974298/148‑1 (MQ=255)
                                                                                                           cttTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGTATCAGTTTTTCCGCCGCCGTATCGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGACACTTTGTTGAt                                   <  1:2057997/148‑1 (MQ=255)
                                                                                                           cttTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGAt                                   <  2:2486476/148‑1 (MQ=255)
                                                                                                           cttTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGAt                                   <  1:1538588/148‑1 (MQ=255)
                                                                                                                 cTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCaca                             >  2:2654666/1‑146 (MQ=255)
                                                                                                                     aGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGata                         <  1:1380281/148‑1 (MQ=255)
                                                                                                                      gTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGataa                        <  2:1425928/148‑1 (MQ=255)
                                                                                                                       tCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGataat                       <  2:2083347/148‑1 (MQ=255)
                                                                                                                          aaaTTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATAtt                    <  1:1782327/148‑1 (MQ=255)
                                                                                                                          aaaTTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATAtt                    <  2:345038/148‑1 (MQ=255)
                                                                                                                            aTTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATACTAttct                  >  2:901877/1‑148 (MQ=255)
                                                                                                                            aTTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATAttct                  >  2:2594823/1‑148 (MQ=255)
                                                                                                                            aTTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATAttct                  >  2:2594831/1‑148 (MQ=255)
                                                                                                                            aTTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATAttct                  <  2:1933395/148‑1 (MQ=255)
                                                                                                                            aTTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATAttct                  <  2:2064826/148‑1 (MQ=255)
                                                                                                                             tttGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATAttctt                 >  2:1290463/1‑148 (MQ=255)
                                                                                                                             tttGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATAttctt                 >  1:1737121/1‑148 (MQ=255)
                                                                                                                              ttGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATAttcttc                >  1:391377/1‑148 (MQ=255)
                                                                                                                              ttGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATAttcttc                >  2:1069695/1‑148 (MQ=255)
                                                                                                                                 ttCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGcc             <  1:2664351/148‑1 (MQ=255)
                                                                                                                                 ttCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGcc             >  1:3070392/1‑148 (MQ=255)
                                                                                                                                     gagaTATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGgt         >  1:1665177/1‑148 (MQ=255)
                                                                                                                                      agaTATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGgta        >  1:2716905/1‑148 (MQ=255)
                                                                                                                                      agaTATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGgta        >  1:2271661/1‑148 (MQ=255)
                                                                                                                                       gaTATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGgtag       <  2:1617080/148‑1 (MQ=255)
                                                                                                                                       gaTATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGgtag       <  2:1617076/148‑1 (MQ=255)
                                                                                                                                        atatCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGgtagt      >  1:1059528/1‑148 (MQ=255)
                                                                                                                                         tatCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGgtagta     <  1:2014397/148‑1 (MQ=255)
                                                                                                                                            ccgcctccgGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGcc  <  1:3228153/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                              
ATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCGCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCC  >  CP009273/3543814‑3544101

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCGCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGT  >  CP009273/3543811‑3544105
                                                                                                                                                    |                                                                                                                                                  
GATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTTTTCGGAGATATCCGCCTC                                                                                                                                                   >  SRR19973070.3422/1‑150 (MQ=60)
GATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTC                                                                                                                                                   <  SRR19973070.2408162/150‑1 (MQ=60)
GATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTAGGAGATATCCGCCTC                                                                                                                                                   >  SRR19973070.3467/1‑150 (MQ=60)
 ATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGNGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCC                                                                                                                                                  >  SRR19973070.1688111/1‑150 (MQ=60)
 ATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCC                                                                                                                                                  >  SRR19973070.1591568/1‑150 (MQ=60)
 ATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCC                                                                                                                                                  >  SRR19973070.1110937/1‑150 (MQ=60)
 ATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCGCC                                                                                                                                                  >  SRR19973070.2640928/1‑150 (MQ=60)
 ATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATACGCCTCC                                                                                                                                                  >  SRR19973070.2097364/1‑150 (MQ=60)
  TATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCCGTGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCG                                                                                                                                                 <  SRR19973070.401377/150‑1 (MQ=60)
  TATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCG                                                                                                                                                 <  SRR19973070.2485575/150‑1 (MQ=60)
   ATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGG                                                                                                                                                <  SRR19973070.1081779/150‑1 (MQ=60)
     TTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGA                                                                                                                                              >  SRR19973070.2556265/1‑150 (MQ=60)
     TTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGA                                                                                                                                              >  SRR19973070.2556256/1‑150 (MQ=60)
       TCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATC                                                                                                                                            <  SRR19973070.1036394/150‑1 (MQ=60)
       TCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATC                                                                                                                                            <  SRR19973070.2699230/150‑1 (MQ=60)
        CTTCACCGACTTTCTCGGCGATTTCATCGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCATTACCTGCAGTAGAAATTTGTACGGAGATAACCGCCTCCGGGATCA                                                                                                                                           >  SRR19973070.824986/1‑150 (MQ=60)
        CTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTTCCTGCAGTCGAAATTTGTTCGGAGATATACGCCTCCGGGATCA                                                                                                                                           >  SRR19973070.850466/1‑150 (MQ=60)
        CTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCA                                                                                                                                           >  SRR19973070.827467/1‑150 (MQ=60)
              CGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTT                                                                                                                                     >  SRR19973070.791289/1‑150 (MQ=60)
              CGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTT                                                                                                                                     >  SRR19973070.791328/1‑150 (MQ=60)
              CGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTT                                                                                                                                     >  SRR19973070.2052299/1‑150 (MQ=60)
              CGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTT                                                                                                                                     >  SRR19973070.791338/1‑150 (MQ=60)
               GACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTC                                                                                                                                    <  SRR19973070.41398/150‑1 (MQ=60)
               GACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTC                                                                                                                                    <  SRR19973070.450116/150‑1 (MQ=60)
                ACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCC                                                                                                                                   <  SRR19973070.2431429/150‑1 (MQ=60)
                ACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCC                                                                                                                                   <  SRR19973070.760058/150‑1 (MQ=60)
                ACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCC                                                                                                                                   <  SRR19973070.759863/150‑1 (MQ=60)
                    TCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCG                                                                                                                               <  SRR19973070.1378947/150‑1 (MQ=60)
                     CTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTTCCTGCAGTCGAAATTTTTTCGGAGATATACGCCGCCGGGATCAGTTTTTACGCCGC                                                                                                                              >  SRR19973070.2798507/1‑150 (MQ=60)
                      TCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCC                                                                                                                             >  SRR19973070.2877852/1‑150 (MQ=60)
                      TCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATACGCCTCCGGGATCAGTTTTTCCGCCGCC                                                                                                                             >  SRR19973070.600153/1‑150 (MQ=60)
                           GATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAAC                                                                                                                        <  SRR19973070.2365200/150‑1 (MQ=60)
                           GATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAAC                                                                                                                        <  SRR19973070.1014236/150‑1 (MQ=60)
                           GATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAAC                                                                                                                        >  SRR19973070.61885/1‑150 (MQ=60)
                           GATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAAC                                                                                                                        <  SRR19973070.693276/150‑1 (MQ=60)
                           GATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTAGGACATATCCGCCTCCGGGATCAGTTTTTCCCCCGCCGAAAA                                                                                                                        >  SRR19973070.832569/1‑150 (MQ=60)
                            ATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGCTCAGTTTTTCCGCCGCCGAAACG                                                                                                                       >  SRR19973070.683181/1‑150 (MQ=60)
                            ATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACG                                                                                                                       >  SRR19973070.2924466/1‑150 (MQ=60)
                            ATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACG                                                                                                                       >  SRR19973070.1310774/1‑150 (MQ=60)
                            ATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACG                                                                                                                       >  SRR19973070.2353258/1‑150 (MQ=60)
                                CAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTTTTCGGAGATATCCGCCTCCGGGCTCAGTTTTTACGCCGCCGAAACGCAAT                                                                                                                   >  SRR19973070.322292/1‑150 (MQ=60)
                                CAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTATTTACCTGCAGTCGAAATTTGTTCGGAGATATACGCCTCCGGGGTCAGTTTTTACGCCGCCGCAACGCAAT                                                                                                                   >  SRR19973070.322284/1‑150 (MQ=60)
                                CAACGTTCGCCCCATCCAGAGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAAT                                                                                                                   <  SRR19973070.2115186/150‑1 (MQ=60)
                                 AACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATA                                                                                                                  >  SRR19973070.1492958/1‑150 (MQ=60)
                                 AACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATA                                                                                                                  >  SRR19973070.459775/1‑150 (MQ=60)
                                     TTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCC                                                                                                              <  SRR19973070.1092672/150‑1 (MQ=60)
                                      TCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCC                                                                                                             >  SRR19973070.1976910/1‑150 (MQ=60)
                                       CGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGG                                                                                                            <  SRR19973070.1477326/150‑1 (MQ=60)
                                       CGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGG                                                                                                            >  SRR19973070.1355155/1‑150 (MQ=60)
                                           CCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGG                                                                                                        >  SRR19973070.2994544/1‑150 (MQ=60)
                                                 AGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACC                                                                                                  <  SRR19973070.1396548/150‑1 (MQ=60)
                                                 AGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACC                                                                                                  <  SRR19973070.3010948/150‑1 (MQ=60)
                                                     TACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCT                                                                                              <  SRR19973070.1281730/150‑1 (MQ=60)
                                                     TACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCT                                                                                              >  SRR19973070.930785/1‑150 (MQ=60)
                                                      ACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTT                                                                                             <  SRR19973070.893473/150‑1 (MQ=60)
                                                         GACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCGTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCACCGGGATCAGTTTTTCCGCCGCCGCAACGCAATAATCCGGCAGGAACACCACCATCAA                                                                                          >  SRR19973070.23103/1‑150 (MQ=60)
                                                            AGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCATCAACTT                                                                                       >  SRR19973070.2078753/1‑150 (MQ=60)
                                                            AGTAAGCGCACCATTGAGCGCCAGTTTCATATTGACGGTACCGGAAGCTTCTTTAACTGCAGTCGAAATTTTTTCGGAGATATACGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAAAAATACAGCAGGAACAAAACCTTCAACAT                                                                                       >  SRR19973070.2078691/1‑150 (MQ=60)
                                                              TAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTAT                                                                                     >  SRR19973070.427884/1‑150 (MQ=60)
                                                              TAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAAACCGGCAGGAACACCACCATCAACTTAT                                                                                     >  SRR19973070.2102858/1‑150 (MQ=60)
                                                               AAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATC                                                                                    >  SRR19973070.3036413/1‑150 (MQ=60)
                                                                         TTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGC                                                                          <  SRR19973070.862299/150‑1 (MQ=60)
                                                                          TGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCG                                                                         <  SRR19973070.1020249/150‑1 (MQ=60)
                                                                                  AGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTAGAAAATTGTTCGGGGAAATCCGCAACCCGGAACAGTTTTTACGACGCCGAAAAGCAATAATACCGCAGGAACACCACCAACAACTTATCGACCACCACCCGATCGTTG                                                                 >  SRR19973070.989533/1‑150 (MQ=48)
                                                                                      TCATATTGCCGGTACCGGAAGCTTCTTTACCTGCACTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGA                                                             >  SRR19973070.1680026/1‑150 (MQ=60)
                                                                                        ATTTTTCCGGTACCGGAAGCTTCTTTACCTTCAGTCGAAATTTGTTCGGAGATTTCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAAATTATCGCCAACCAGCGGATCGTTGTTGATC                                                           <  SRR19973070.1491641/150‑1 (MQ=60)
                                                                                        ATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATC                                                           >  SRR19973070.834927/1‑150 (MQ=60)
                                                                                        ATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATC                                                           <  SRR19973070.2229245/150‑1 (MQ=60)
                                                                                             GCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTC                                                      <  SRR19973070.2764752/150‑1 (MQ=60)
                                                                                                  TACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCA                                                 >  SRR19973070.2738697/1‑150 (MQ=60)
                                                                                                         AGCTTCTTTACCTGCTGTCGAAATTTGTTCGGAGATATCCGCCTCCTGGATCAGTTTTTCCGCCGCGGAATCGCAATACTCCGGCACGAACACCACCTTCAACTTATCTCCTACCAGCGGATCGTTGTTGATCACGTCCGCCACTTTGTT                                          <  SRR19973070.1293825/150‑1 (MQ=60)
                                                                                                          GCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTG                                         <  SRR19973070.1944199/150‑1 (MQ=60)
                                                                                                              CTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGTATCAGTTTTTCCGCCGCCGTATCGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGACACTTTGTTGATCG                                     <  SRR19973070.2071890/150‑1 (MQ=60)
                                                                                                              CTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCG                                     <  SRR19973070.1549404/150‑1 (MQ=60)
                                                                                                                   CCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAG                                >  SRR19973070.2810121/1‑150 (MQ=60)
                                                                                                                        AGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAAT                           <  SRR19973070.1389638/150‑1 (MQ=60)
                                                                                                                        AGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAAT                           <  SRR19973070.1389644/150‑1 (MQ=60)
                                                                                                                             AAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCT                      <  SRR19973070.1795016/150‑1 (MQ=60)
                                                                                                                             AAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCT                      <  SRR19973070.1794658/150‑1 (MQ=60)
                                                                                                                              AATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTT                     <  SRR19973070.126078/150‑1 (MQ=60)
                                                                                                                              AATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTT                     >  SRR19973070.1749156/1‑150 (MQ=60)
                                                                                                                              AATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTT                     >  SRR19973070.1749149/1‑150 (MQ=60)
                                                                                                                               ATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTC                    >  SRR19973070.394252/1‑150 (MQ=60)
                                                                                                                                TTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCG                   <  SRR19973070.3092779/150‑1 (MQ=60)
                                                                                                                                  TGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCC                 >  SRR19973070.3090094/1‑150 (MQ=60)
                                                                                                                                    TTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAG               <  SRR19973070.2681665/150‑1 (MQ=60)
                                                                                                                                      CGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGT             >  SRR19973070.1676512/1‑150 (MQ=60)
                                                                                                                                      CGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGT             >  SRR19973070.1676622/1‑150 (MQ=60)
                                                                                                                                      CGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGT             >  SRR19973070.744220/1‑150 (MQ=60)
                                                                                                                                       GGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTA            >  SRR19973070.2734448/1‑150 (MQ=60)
                                                                                                                                       GGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTA            >  SRR19973070.2286746/1‑150 (MQ=60)
                                                                                                                                         AGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGT          >  SRR19973070.1066962/1‑150 (MQ=60)
                                                                                                                                            TATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGC       <  SRR19973070.2028021/150‑1 (MQ=60)
                                                                                                                                               CCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCG    <  SRR19973070.3249069/150‑1 (MQ=60)
                                                                                                                                               CCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCG    <  SRR19973070.3249004/150‑1 (MQ=60)
                                                                                                                                                 GCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGT  >  SRR19973070.2929982/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                  
GATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCGCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGT  >  CP009273/3543811‑3544105

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑