Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,543,959 G→T 100% A625E (GCG→GAG)  malP ← maltodextrin phosphorylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,543,9590GT100.0% 691.7 / ‑6.7 227A625E (GCG→GAG) malPmaltodextrin phosphorylase
Reads supporting (aligned to +/- strand):  ref base G (4/0);  new base T (121/101);  total (126/101)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 234 total aligned reads displayed.

TTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCGCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCC  >  CP009273/3543816‑3544101
                                                                                                                                               |                                                                                                                                              
 ttcttcACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACAGGAAGCTTCTGTACCTGCAGTCGAAATTGGTTCGGAGATATCCGCCTCCggg                                                                                                                                           <  1:2975645/148‑1 (MQ=255)
  tcttcACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTGACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGa                                                                                                                                          <  2:2700940/148‑1 (MQ=255)
   cttcACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGAt                                                                                                                                         <  1:2980254/148‑1 (MQ=255)
   cttcACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGAt                                                                                                                                         <  1:2979951/148‑1 (MQ=255)
     tcACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCa                                                                                                                                       >  1:2071294/1‑148 (MQ=255)
     tcACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCa                                                                                                                                       <  2:2910042/148‑1 (MQ=255)
     tcACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCa                                                                                                                                       >  1:1862869/1‑148 (MQ=255)
     tcACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCACCGGGATCa                                                                                                                                       >  2:1765977/1‑148 (MQ=255)
       aCCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGt                                                                                                                                     >  1:1258962/1‑148 (MQ=255)
       aCCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGt                                                                                                                                     >  1:1259430/1‑148 (MQ=255)
       aCCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGt                                                                                                                                     <  1:803246/148‑1 (MQ=255)
         cGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGttt                                                                                                                                   <  2:973071/148‑1 (MQ=255)
         cGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGttt                                                                                                                                   <  1:3597521/148‑1 (MQ=255)
         cGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGttt                                                                                                                                   <  2:3003782/148‑1 (MQ=255)
         cGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATAGCCGGTACCGGAAGCTTATTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGttt                                                                                                                                   >  2:3695642/1‑148 (MQ=255)
           aCTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGttttt                                                                                                                                 >  1:2901253/1‑148 (MQ=255)
           aCTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGAAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGttttt                                                                                                                                 >  2:1858373/1‑148 (MQ=255)
           aCTATCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTAACTTCAGTCGAAAATTGTTATGAGATATACGCCTACGGGCTCAGatttt                                                                                                                                 >  1:2901226/1‑148 (MQ=255)
            cTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCCTTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTc                                                                                                                                >  2:2939990/1‑148 (MQ=255)
            cTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTc                                                                                                                                <  2:2717202/148‑1 (MQ=255)
             tttCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTcc                                                                                                                               >  1:3809241/1‑148 (MQ=255)
              ttCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTccg                                                                                                                              >  1:3761823/1‑148 (MQ=255)
                       aTTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGaaa                                                                                                                     >  2:3062211/1‑148 (MQ=255)
                       aTTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGaaa                                                                                                                     >  2:544917/1‑148 (MQ=255)
                        tttCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAAc                                                                                                                    >  2:1575830/1‑148 (MQ=255)
                           cAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCa                                                                                                                 >  2:2849098/1‑148 (MQ=255)
                             aCGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCaat                                                                                                               >  1:2147489/1‑148 (MQ=255)
                              cGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCaata                                                                                                              >  2:3448302/1‑148 (MQ=255)
                                 tCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATc                                                                                                           <  1:1445915/148‑1 (MQ=255)
                                    ccccATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTGCCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGCTCAGTTTTTCCGCCGCCGAAACGCAATAATCCgg                                                                                                        >  1:2829593/1‑148 (MQ=255)
                                         tCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGa                                                                                                   >  2:770542/1‑148 (MQ=255)
                                          ccAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGaa                                                                                                  >  2:1280608/1‑148 (MQ=255)
                                          ccAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGaa                                                                                                  >  1:10504/1‑148 (MQ=255)
                                           cAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAac                                                                                                 <  1:3530915/148‑1 (MQ=255)
                                                   cGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCtt                                                                                         >  1:3165104/1‑148 (MQ=255)
                                                      cAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCaa                                                                                      <  2:610120/148‑1 (MQ=255)
                                                        gTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACt                                                                                    >  2:241880/1‑148 (MQ=255)
                                                         tAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACtt                                                                                   <  1:2773649/148‑1 (MQ=255)
                                                           aGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTAt                                                                                 <  2:917964/148‑1 (MQ=255)
                                                               cACCATTGAGCGCCAGATTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATACGGCAGGAACACCACCTTCAAATTAACGcc                                                                             >  2:3505167/1‑148 (MQ=255)
                                                                aCCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCa                                                                            >  2:172201/1‑148 (MQ=255)
                                                                   aTTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAAcc                                                                         <  2:742497/148‑1 (MQ=255)
                                                                    ttGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCa                                                                        >  2:1871684/1‑148 (MQ=255)
                                                                         cgcCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTAGGAGATATACGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATAGCCAACCAGCGGa                                                                   >  1:1797073/1‑148 (MQ=255)
                                                                         cgcCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAAATTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCCCCGAAACGCAATAATCCGGCAGGAACACCACCATCAACTTATCGCCAACCACCGGa                                                                   >  1:3689698/1‑148 (MQ=255)
                                                                         cgcCAGTTTCATATTGCCGGTACCGGAAGCGTCTTTACCTGCAGTCGAAATTTGTTCGGAGTTATCCGCCGCCGGGCTCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACATATCGCCCACCAGCGGa                                                                   >  1:1123948/1‑148 (MQ=255)
                                                                              gTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCgtt                                                              <  2:917410/148‑1 (MQ=255)
                                                                               tttCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCgttg                                                             >  2:3646852/1‑148 (MQ=255)
                                                                               tttCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCgttg                                                             <  1:1505691/148‑1 (MQ=255)
                                                                                ttCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCgttgt                                                            >  1:503820/1‑148 (MQ=255)
                                                                                   atatTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAACTTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGa                                                         <  2:394599/148‑1 (MQ=255)
                                                                                   atatTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGa                                                         >  2:2407969/1‑148 (MQ=255)
                                                                                    tatTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGAt                                                        <  1:737220/148‑1 (MQ=255)
                                                                                      ttGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGCTCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCa                                                      >  2:662117/1‑148 (MQ=255)
                                                                                      ttGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCa                                                      >  2:3540386/1‑148 (MQ=255)
                                                                                             tACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGc                                               <  1:797048/148‑1 (MQ=255)
                                                                                                 ggAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACt                                           <  2:2810965/148‑1 (MQ=255)
                                                                                                  gAAGCTTCTTGACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACtt                                          <  2:847844/148‑1 (MQ=255)
                                                                                                   aaGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACttt                                         >  1:563571/1‑148 (MQ=255)
                                                                                                   aaGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACttt                                         >  2:2029745/1‑148 (MQ=255)
                                                                                                    aGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAAGAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTttg                                        <  2:814237/148‑1 (MQ=255)
                                                                                                     gCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTttgt                                       >  1:882621/1‑148 (MQ=255)
                                                                                                       ttcttTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTttgttt                                     >  2:2917779/1‑147 (MQ=255)
                                                                                                       ttcttTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTttgttg                                     <  2:309355/148‑1 (MQ=255)
                                                                                                       ttcttTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTttgttg                                     <  2:1509419/148‑1 (MQ=255)
                                                                                                       ttcttTACCTGCAGTCGAAATTTGTTCGGAGATATCCACCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTttgttg                                     >  1:2149962/1‑148 (MQ=255)
                                                                                                       ttcttTACCTGCAGTCGAAATTTGTTCGGAGATATCCACCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTttgttg                                     >  1:2149230/1‑148 (MQ=255)
                                                                                                        tcttGACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGa                                    <  1:2412947/148‑1 (MQ=255)
                                                                                                           ttACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCg                                 >  2:3659206/1‑148 (MQ=255)
                                                                                                              ccTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCaa                              >  2:1146899/1‑148 (MQ=255)
                                                                                                              ccTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCaa                              <  1:2535238/148‑1 (MQ=255)
                                                                                                              ccTGCAGTCGAAATGTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAAGCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCaa                              <  1:1657827/148‑1 (MQ=255)
                                                                                                                  cAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGat                          >  1:2210766/1‑148 (MQ=255)
                                                                                                                    gTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGataa                        >  1:3476892/1‑148 (MQ=255)
                                                                                                                     tCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGataat                       >  2:92726/1‑148 (MQ=255)
                                                                                                                     tCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGataat                       <  1:247633/148‑1 (MQ=255)
                                                                                                                     gcGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGataat                       <  2:772774/147‑1 (MQ=255)
                                                                                                                      cGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGataata                      <  2:1105175/148‑1 (MQ=255)
                                                                                                                      cGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGataata                      <  2:220478/148‑1 (MQ=255)
                                                                                                                        aaaTTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATAtt                    <  2:1256633/148‑1 (MQ=255)
                                                                                                                         actttGTTCGGAGATATCCCCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATAttc                   <  2:2526083/146‑1 (MQ=255)
                                                                                                                          aTTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATAttct                  >  1:1595577/1‑148 (MQ=255)
                                                                                                                           tttGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATAttctt                 >  1:3408240/1‑148 (MQ=255)
                                                                                                                            ttGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATAttcttc                >  2:2826949/1‑148 (MQ=255)
                                                                                                                             tGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCg               >  1:1284259/1‑148 (MQ=255)
                                                                                                                                tCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCa            >  2:221073/1‑148 (MQ=255)
                                                                                                                                tCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCa            <  1:1009506/148‑1 (MQ=255)
                                                                                                                                   gagaTATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGgt         >  2:1459885/1‑148 (MQ=255)
                                                                                                                                   gagaTATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGgt         >  2:2608371/1‑148 (MQ=255)
                                                                                                                                   gagaTATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGgt         <  1:921097/148‑1 (MQ=255)
                                                                                                                                   gagaTATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGgt         >  1:1947712/1‑148 (MQ=255)
                                                                                                                                    agaTATCCGCCTCCGGGGTCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGgta        >  2:321436/1‑148 (MQ=255)
                                                                                                                                     gaTATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGgtag       <  1:58487/148‑1 (MQ=255)
                                                                                                                                     gaTATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGgtag       <  1:3099051/148‑1 (MQ=255)
                                                                                                                                      atatCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGgtagt      >  2:2102585/1‑148 (MQ=255)
                                                                                                                                        atCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGtagtag    >  2:672192/1‑148 (MQ=255)
                                                                                                                                         tCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGc   >  2:2469211/1‑148 (MQ=255)
                                                                                                                                          ccgcctccgGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGcc  >  1:2328512/1‑148 (MQ=255)
                                                                                                                                          ccgcctccgGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGcc  >  1:1555940/1‑148 (MQ=255)
                                                                                                                                          ccgcctccgGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGcc  <  2:2720387/148‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                              
TTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCGCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCC  >  CP009273/3543816‑3544101

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCGCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCC  >  CP009273/3543811‑3544108
                                                                                                                                                    |                                                                                                                                                     
GATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTC                                                                                                                                                      >  SRR19973069.747755/1‑150 (MQ=60)
GATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTC                                                                                                                                                      >  SRR19973069.1346095/1‑150 (MQ=60)
GATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCGC                                                                                                                                                      >  SRR19973069.747817/1‑150 (MQ=60)
      TTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACAGGAAGCTTCTGTACCTGCAGTCGAAATTGGTTCGGAGATATCCGCCTCCGGGAT                                                                                                                                                <  SRR19973069.2995409/150‑1 (MQ=60)
        CTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGATAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCA                                                                                                                                              <  SRR19973069.3000041/150‑1 (MQ=60)
        CTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCA                                                                                                                                              >  SRR19973069.2085647/1‑150 (MQ=60)
        CTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCA                                                                                                                                              >  SRR19973069.1875863/1‑150 (MQ=60)
        CTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCA                                                                                                                                              <  SRR19973069.2999734/150‑1 (MQ=60)
        CTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCA                                                                                                                                              <  SRR19973069.3000034/150‑1 (MQ=60)
        CTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCA                                                                                                                                              <  SRR19973069.3000039/150‑1 (MQ=60)
          TCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGT                                                                                                                                            >  SRR19973069.1267457/1‑150 (MQ=60)
          TCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGT                                                                                                                                            >  SRR19973069.1267731/1‑150 (MQ=60)
          TCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGT                                                                                                                                            >  SRR19973069.1267927/1‑150 (MQ=60)
          TCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGT                                                                                                                                            >  SRR19973069.3780907/1‑150 (MQ=60)
           CACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTT                                                                                                                                           <  SRR19973069.2542162/150‑1 (MQ=60)
            ACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTT                                                                                                                                          <  SRR19973069.808943/150‑1 (MQ=60)
              CGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTTTTAGGAGATATACGCCTCCGGGATCAGTTTTT                                                                                                                                        >  SRR19973069.2920414/1‑150 (MQ=60)
              CGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTT                                                                                                                                        <  SRR19973069.3621005/150‑1 (MQ=60)
              CGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTT                                                                                                                                        >  SRR19973069.2920416/1‑150 (MQ=60)
              CGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTAACTGCAGTCGAAATTTGTTAGGAGATATCCGCCTCCGGGCTCAGTTTTT                                                                                                                                        >  SRR19973069.2920578/1‑150 (MQ=60)
               GACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTC                                                                                                                                       <  SRR19973069.1194748/150‑1 (MQ=60)
               GACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATACGCCTCCGGGATCAGTTTTTA                                                                                                                                       >  SRR19973069.2795195/1‑150 (MQ=60)
                ACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCC                                                                                                                                      >  SRR19973069.3833901/1‑150 (MQ=60)
                 CTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTGTTTACCTGCAGTCGAAATTTGTTTGGAGATATACGCCTCCGGGATCAGTTTTTGCG                                                                                                                                     >  SRR19973069.1626337/1‑150 (MQ=60)
                 CTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTTTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCG                                                                                                                                     >  SRR19973069.358132/1‑150 (MQ=60)
                 CTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCG                                                                                                                                     >  SRR19973069.3786278/1‑150 (MQ=60)
                                CAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAAT                                                                                                                      >  SRR19973069.2162211/1‑150 (MQ=60)
                                CAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAAT                                                                                                                      <  SRR19973069.3828914/150‑1 (MQ=60)
                                CAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAAT                                                                                                                      >  SRR19973069.220177/1‑150 (MQ=60)
                                   CGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAAT                                                                                                                   <  SRR19973069.2918716/150‑1 (MQ=60)
                                   CGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTAGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAAT                                                                                                                   <  SRR19973069.2137696/150‑1 (MQ=60)
                                      TCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCG                                                                                                                <  SRR19973069.1456004/150‑1 (MQ=60)
                                       CGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTGCCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGCTCAGTTTTTCCGCCGCCGAAACGCAATAATCCGG                                                                                                               >  SRR19973069.2848291/1‑150 (MQ=60)
                                       CGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTGGGAGATATACGCCGCCGGGGTCAGTTTTTGCGCCGCCGAAAAGCAATAATACGG                                                                                                               >  SRR19973069.982343/1‑150 (MQ=60)
                                       CGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGG                                                                                                               >  SRR19973069.2847165/1‑150 (MQ=60)
                                          CCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTTTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCAGGCAG                                                                                                            >  SRR19973069.673525/1‑150 (MQ=60)
                                             ATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGGTCAGTTTTTCCGCCGCCGAAACGCAATAATACGGCAGGAA                                                                                                         >  SRR19973069.10660/1‑150 (MQ=60)
                                              TCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAAC                                                                                                        <  SRR19973069.501285/150‑1 (MQ=60)
                                                CAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACAC                                                                                                      <  SRR19973069.3553775/150‑1 (MQ=60)
                                                CAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACAC                                                                                                      <  SRR19973069.58881/150‑1 (MQ=60)
                                                CAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACAC                                                                                                      >  SRR19973069.868574/1‑150 (MQ=60)
                                                CAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTAACTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACAC                                                                                                      >  SRR19973069.868400/1‑150 (MQ=60)
                                                   CGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAAATTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATACGGCAGGAACACCAC                                                                                                   >  SRR19973069.1765089/1‑150 (MQ=60)
                                                      ACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTT                                                                                                >  SRR19973069.3185739/1‑150 (MQ=60)
                                                          ACAGTAAGCGCACCATTGAGCGACAGTTTAATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCATGAACACCACCTTCAAC                                                                                            <  SRR19973069.1112921/150‑1 (MQ=60)
                                                              TAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTAT                                                                                        <  SRR19973069.2792123/150‑1 (MQ=60)
                                                              TAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTAT                                                                                        <  SRR19973069.1463074/150‑1 (MQ=60)
                                                               AAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATC                                                                                       >  SRR19973069.1252328/1‑150 (MQ=60)
                                                                          TGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCGCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCG                                                                            >  SRR19973069.2995712/1‑150 (MQ=60)
                                                                            AGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGA                                                                          >  SRR19973069.1286265/1‑150 (MQ=60)
                                                                            AGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTAGGAGATATACGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATAGCCAACCAGCGGA                                                                          >  SRR19973069.1809728/1‑150 (MQ=60)
                                                                            AGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAAATTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCCCCGAAACGCAATAATCCGGCAGGAACACCACCATCAACTTATCGCCAACCACCGGA                                                                          >  SRR19973069.3713830/1‑150 (MQ=60)
                                                                            AGCGCCAGTTTCATATTGCCGGTACCGGAAGCGTCTTTACCTGCAGTCGAAATTTGTTCGGAGTTATCCGCCGCCGGGCTCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACATATCGCCCACCAGCGGA                                                                          >  SRR19973069.1131786/1‑150 (MQ=60)
                                                                                   GTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGT                                                                   <  SRR19973069.2168673/150‑1 (MQ=60)
                                                                                   GTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGT                                                                   >  SRR19973069.507428/1‑150 (MQ=60)
                                                                                    TTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTT                                                                  <  SRR19973069.1516122/150‑1 (MQ=60)
                                                                                       CATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGAT                                                               <  SRR19973069.2154667/150‑1 (MQ=60)
                                                                                         TATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCA                                                             <  SRR19973069.742441/150‑1 (MQ=60)
                                                                                          ATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCACTAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCAC                                                            >  SRR19973069.2242955/1‑150 (MQ=60)
                                                                                          ATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCAC                                                            <  SRR19973069.3221360/150‑1 (MQ=60)
                                                                                          ATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCAC                                                            >  SRR19973069.303881/1‑150 (MQ=60)
                                                                                              CCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCA                                                        <  SRR19973069.206928/150‑1 (MQ=60)
                                                                                              CCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCA                                                        <  SRR19973069.1574614/150‑1 (MQ=60)
                                                                                                 GTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCC                                                     <  SRR19973069.1841414/150‑1 (MQ=60)
                                                                                                  TACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCA                                                    <  SRR19973069.802758/150‑1 (MQ=60)
                                                                                                  TACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCA                                                    <  SRR19973069.802699/150‑1 (MQ=60)
                                                                                                      GGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTT                                                >  SRR19973069.567498/1‑150 (MQ=60)
                                                                                                      GGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCGCCGGGATCAGTTTTTCCGCCGCCGAAACGCAAAAATACGGCAGGAACACCACCTTCAACATATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTT                                                >  SRR19973069.415243/1‑150 (MQ=60)
                                                                                                        AAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGT                                              >  SRR19973069.888734/1‑150 (MQ=60)
                                                                                                        AAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGT                                              >  SRR19973069.2478531/1‑150 (MQ=60)
                                                                                                          GCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCACCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTG                                            >  SRR19973069.2164694/1‑150 (MQ=60)
                                                                                                          GCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCACCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTG                                            >  SRR19973069.2164060/1‑150 (MQ=60)
                                                                                                          GCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCACCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTG                                            >  SRR19973069.2163946/1‑150 (MQ=60)
                                                                                                          GCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCACCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTG                                            >  SRR19973069.2163960/1‑150 (MQ=60)
                                                                                                           CTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGT                                           >  SRR19973069.1325821/1‑150 (MQ=60)
                                                                                                             TCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATC                                         <  SRR19973069.3153985/150‑1 (MQ=60)
                                                                                                             TCTTGACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATC                                         <  SRR19973069.2429261/150‑1 (MQ=60)
                                                                                                                   CCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAG                                   <  SRR19973069.2552034/150‑1 (MQ=60)
                                                                                                                   CCTGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAG                                   <  SRR19973069.2552075/150‑1 (MQ=60)
                                                                                                                   CCTGCAGTCGAAATGTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAAGCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAG                                   <  SRR19973069.1669305/150‑1 (MQ=60)
                                                                                                                     TGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGAT                                 <  SRR19973069.369130/150‑1 (MQ=60)
                                                                                                                     TGCAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGAT                                 >  SRR19973069.2225746/1‑150 (MQ=60)
                                                                                                                       CAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAA                               >  SRR19973069.3499350/1‑150 (MQ=60)
                                                                                                                       CAGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAA                               >  SRR19973069.3499366/1‑150 (MQ=60)
                                                                                                                        AGTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAAT                              >  SRR19973069.1861754/1‑150 (MQ=60)
                                                                                                                         GTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATA                             <  SRR19973069.1042092/150‑1 (MQ=60)
                                                                                                                         GTCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATA                             <  SRR19973069.747891/150‑1 (MQ=60)
                                                                                                                          TCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATAT                            <  SRR19973069.249463/150‑1 (MQ=60)
                                                                                                                          TCGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATAT                            <  SRR19973069.248685/150‑1 (MQ=60)
                                                                                                                           CGAAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATT                           <  SRR19973069.1014153/150‑1 (MQ=60)
                                                                                                                           CGAAATTTGTTAGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATTATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATT                           <  SRR19973069.3527726/150‑1 (MQ=60)
                                                                                                                             AAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCT                         >  SRR19973069.1606314/1‑150 (MQ=60)
                                                                                                                             AAATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCT                         >  SRR19973069.1606348/1‑150 (MQ=60)
                                                                                                                              AATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTT                        >  SRR19973069.3430456/1‑150 (MQ=60)
                                                                                                                              AATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTT                        >  SRR19973069.1541337/1‑150 (MQ=60)
                                                                                                                               ATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTC                       <  SRR19973069.3699576/150‑1 (MQ=60)
                                                                                                                               ATTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTC                       <  SRR19973069.2959833/150‑1 (MQ=60)
                                                                                                                                TTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCG                      >  SRR19973069.1292848/1‑150 (MQ=60)
                                                                                                                                TTTGTTCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCG                      >  SRR19973069.1293192/1‑150 (MQ=60)
                                                                                                                                     TCGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGG                 <  SRR19973069.1016458/150‑1 (MQ=60)
                                                                                                                                      CGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGT                >  SRR19973069.1961166/1‑150 (MQ=60)
                                                                                                                                      CGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGT                <  SRR19973069.548740/150‑1 (MQ=60)
                                                                                                                                      CGGAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGT                >  SRR19973069.1960843/1‑150 (MQ=60)
                                                                                                                                        GAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAG              <  SRR19973069.927370/150‑1 (MQ=60)
                                                                                                                                        GAGATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAG              <  SRR19973069.926983/150‑1 (MQ=60)
                                                                                                                                          GATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTA            <  SRR19973069.695554/150‑1 (MQ=60)
                                                                                                                                          GATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTA            <  SRR19973069.3119468/150‑1 (MQ=60)
                                                                                                                                          GATATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTA            <  SRR19973069.59193/150‑1 (MQ=60)
                                                                                                                                             ATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGTCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCC         >  SRR19973069.2349305/1‑150 (MQ=60)
                                                                                                                                             ATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCC         >  SRR19973069.3477688/1‑150 (MQ=60)
                                                                                                                                             ATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCC         >  SRR19973069.1566572/1‑150 (MQ=60)
                                                                                                                                             ATCCGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCC         >  SRR19973069.2344350/1‑150 (MQ=60)
                                                                                                                                                CGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGG      >  SRR19973069.42935/1‑150 (MQ=60)
                                                                                                                                                CGCCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGG      >  SRR19973069.40344/1‑150 (MQ=60)
                                                                                                                                                  CCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTG    <  SRR19973069.2582812/150‑1 (MQ=60)
                                                                                                                                                  CCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTG    >  SRR19973069.1476679/1‑150 (MQ=60)
                                                                                                                                                  CCTCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTG    >  SRR19973069.1452771/1‑150 (MQ=60)
                                                                                                                                                    TCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTTCC  <  SRR19973069.3719801/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
GATATTTTCTTCACCGACTTTCTCGGCGATTTCAACGTTCGCCCCATCCAGCGTACCGACAGTAAGCGCACCATTGAGCGCCAGTTTCATATTGCCGGTACCGGAAGCTTCTTTACCTGCAGTCGAAATTTGTTCGGAGATATCCGCCGCCGGGATCAGTTTTTCCGCCGCCGAAACGCAATAATCCGGCAGGAACACCACCTTCAACTTATCGCCAACCAGCGGATCGTTGTTGATCACGTCAGCCACTTTGTTGATCGCAAAGATAATATTCTTCGCCAGGTAGTAGCCCGGTGCC  >  CP009273/3543811‑3544108

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑