Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 477,267 G→T 100% Q865K (CAG→AAG)  acrB ← multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273477,2670GT98.8% 563.9 / ‑1.8 173Q865K (CAG→AAG) acrBmultidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (94/77);  total (95/78)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 174 total aligned reads displayed.

ACGCACCGATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTC  >  CP009273/477123‑477411
                                                                                                                                                |                                                                                                                                                
aCGCACCGATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTtata                                                                                                                                               >  1:1299451/1‑148 (MQ=255)
 cGCACCGATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAg                                                                                                                                              >  1:86399/1‑148 (MQ=255)
 cGCACCGATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAg                                                                                                                                              <  2:2073459/148‑1 (MQ=255)
 cGCACCGATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAg                                                                                                                                              >  2:340436/1‑148 (MQ=255)
 cGCACCGATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAg                                                                                                                                              >  2:340523/1‑148 (MQ=255)
    aCCGATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGAc                                                                                                                                           >  2:2298477/1‑148 (MQ=255)
      cGATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACAt                                                                                                                                         <  2:277397/148‑1 (MQ=255)
       gATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGACTCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATc                                                                                                                                        <  2:2734341/148‑1 (MQ=255)
       gATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGACTCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATc                                                                                                                                        <  2:2175262/148‑1 (MQ=255)
        aTAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATcc                                                                                                                                       >  2:1744862/1‑148 (MQ=255)
        aTAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATcc                                                                                                                                       >  2:3039525/1‑148 (MQ=255)
         tAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATccc                                                                                                                                      <  1:220407/148‑1 (MQ=255)
                 aGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGt                                                                                                                              <  2:1033168/148‑1 (MQ=255)
                        cGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGcc                                                                                                                       >  2:1923226/1‑148 (MQ=255)
                        cGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGcc                                                                                                                       >  2:3069546/1‑148 (MQ=255)
                        cGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGcc                                                                                                                       >  2:1923314/1‑148 (MQ=255)
                          aCCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCaa                                                                                                                     <  1:3299206/148‑1 (MQ=255)
                          aCCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCaa                                                                                                                     >  2:34597/1‑148 (MQ=255)
                          aCCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCaa                                                                                                                     <  1:1889297/148‑1 (MQ=255)
                          aCCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCaa                                                                                                                     <  1:3298946/148‑1 (MQ=255)
                           ccAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAac                                                                                                                    >  1:188299/1‑148 (MQ=255)
                             aGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAacac                                                                                                                  <  1:93933/148‑1 (MQ=255)
                                aTAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCgg                                                                                                               >  1:1600210/1‑148 (MQ=255)
                                  aaCGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTa                                                                                                             >  2:1868801/1‑148 (MQ=255)
                                   aCGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAg                                                                                                            >  1:1085348/1‑148 (MQ=255)
                                    ccgagaACGGAATCGACAAGCTCTCGTACAGCTCCTCCACACACAGGAACACGACAAACAAAGAAATCGCGAACAGTGAAGGTGCCTGGTTCCCTGAGAGACGATCCTTATAGTACAACCCCGTCCAGTCATAGCCAACACCCGTAgg                                                                                                           >  2:2383495/3‑148 (MQ=255)
                                    cGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAgg                                                                                                           >  2:350592/1‑148 (MQ=255)
                                       agaACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAg                                                                                                        <  1:2240822/148‑1 (MQ=255)
                                        gaACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGt                                                                                                       <  2:670414/148‑1 (MQ=255)
                                        gaACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGt                                                                                                       <  2:1444910/148‑1 (MQ=255)
                                         aaCGGACTCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCTGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGtt                                                                                                      <  1:932120/148‑1 (MQ=255)
                                         aaCGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGtt                                                                                                      <  2:90535/148‑1 (MQ=255)
                                           cGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTg                                                                                                    >  2:2887736/1‑148 (MQ=255)
                                             gAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCt                                                                                                  >  1:3063678/1‑148 (MQ=255)
                                              aaTCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTc                                                                                                 >  2:1106561/1‑148 (MQ=255)
                                               aTCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCg                                                                                                <  2:1188868/148‑1 (MQ=255)
                                                   aCCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAg                                                                                            >  2:1220179/1‑148 (MQ=255)
                                                       gCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAgttgt                                                                                        <  2:124652/148‑1 (MQ=255)
                                                       gCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAgttgt                                                                                        <  2:2894214/148‑1 (MQ=255)
                                                        ctctCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAgttgtt                                                                                       >  1:677349/1‑148 (MQ=255)
                                                            cGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCAt                                                                                   <  1:836224/148‑1 (MQ=255)
                                                            cGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCAt                                                                                   >  1:370234/1‑148 (MQ=255)
                                                              tACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCa                                                                                 >  1:776201/1‑148 (MQ=255)
                                                                 aGCGCCGCCAGACACAGGAACACGACAATCAACGACATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCt                                                                              <  2:996054/148‑1 (MQ=255)
                                                                   cgccgcCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTcc                                                                            <  1:1218373/148‑1 (MQ=255)
                                                                   cgccgcCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTcc                                                                            >  2:1975783/1‑148 (MQ=255)
                                                                   cgccgcCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTcc                                                                            >  2:3235599/1‑148 (MQ=255)
                                                                   cgccgcCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGGAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTcc                                                                            >  2:1941177/1‑148 (MQ=255)
                                                                     ccgccAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCAt                                                                          >  1:1662085/1‑148 (MQ=255)
                                                                      cgccAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCAtt                                                                         >  1:37867/1‑148 (MQ=255)
                                                                        ccAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGc                                                                       >  2:2634581/1‑148 (MQ=255)
                                                                        ccAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGc                                                                       >  2:853430/1‑148 (MQ=255)
                                                                          aGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCtt                                                                     <  2:2714114/148‑1 (MQ=255)
                                                                               caGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCg                                                                <  1:2608631/148‑1 (MQ=255)
                                                                                   aaCACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTAc                                                            <  2:702576/148‑1 (MQ=255)
                                                                                        gACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTa                                                       <  2:949588/148‑1 (MQ=255)
                                                                                            aTCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCg                                                   >  2:3382762/1‑148 (MQ=255)
                                                                                             tCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCgg                                                  >  2:1340724/1‑148 (MQ=255)
                                                                                             tCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCgg                                                  >  2:1156691/1‑148 (MQ=255)
                                                                                              cAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGt                                                 >  2:737030/1‑148 (MQ=255)
                                                                                                  gAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTgccg                                             >  2:2959149/1‑148 (MQ=255)
                                                                                                  gAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTgccg                                             >  1:2466706/1‑148 (MQ=255)
                                                                                                   aaaTCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTgccgc                                            <  1:3415403/148‑1 (MQ=255)
                                                                                                   aaaTCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTgccgc                                            <  1:2862557/148‑1 (MQ=255)
                                                                                                   aaaTCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTgccgc                                            >  1:2780798/1‑148 (MQ=255)
                                                                                                   aaaTCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTgccgc                                            <  1:2235001/148‑1 (MQ=255)
                                                                                                    aaTCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTgccgcc                                           <  2:912605/148‑1 (MQ=255)
                                                                                                     aTCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCt                                          >  1:1307362/1‑148 (MQ=255)
                                                                                                     aTCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCt                                          >  1:2959516/1‑148 (MQ=255)
                                                                                                     aTCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCt                                          <  1:1669517/148‑1 (MQ=255)
                                                                                                     aTCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCt                                          <  1:737030/148‑1 (MQ=255)
                                                                                                        gcgTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGc                                       <  1:58385/148‑1 (MQ=255)
                                                                                                        gcgTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGc                                       >  2:865/1‑148 (MQ=255)
                                                                                                           tACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTAGCTCGCCAGTTGTTCCATCAGCTCCATTGCATCACCGGAACTTTTACCCGGAGCAGCCTGGCCTa                                    >  2:3538193/1‑148 (MQ=255)
                                                                                                            aCAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTaa                                   >  1:2436177/1‑148 (MQ=255)
                                                                                                               gTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGAt                                <  2:232179/148‑1 (MQ=255)
                                                                                                               gTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGAt                                <  1:765114/148‑1 (MQ=255)
                                                                                                                tGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGAtt                               >  2:1539741/1‑148 (MQ=255)
                                                                                                                 gAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGAttt                              <  1:616984/148‑1 (MQ=255)
                                                                                                                     gTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCat                          <  1:347253/148‑1 (MQ=255)
                                                                                                                        ccTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCatgga                       <  1:1106561/148‑1 (MQ=255)
                                                                                                                         cTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCatggat                      <  2:3556982/148‑1 (MQ=255)
                                                                                                                               gCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAgg                <  2:1570095/148‑1 (MQ=255)
                                                                                                                                ccGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGc               >  2:1838709/1‑148 (MQ=255)
                                                                                                                                    agagaCGGTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGtt           <  2:2077433/148‑1 (MQ=255)
                                                                                                                                      agaCGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGt         >  1:2667897/1‑148 (MQ=255)
                                                                                                                                      agaCGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGt         >  1:2668401/1‑148 (MQ=255)
                                                                                                                                       gaCGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTa        <  2:109300/148‑1 (MQ=255)
                                                                                                                                       gaCGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATCGATGGCAGGCCGATGTa        >  2:2243890/1‑148 (MQ=255)
                                                                                                                                        aCGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTaa       >  1:2491902/1‑148 (MQ=255)
                                                                                                                                        aCGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTaa       >  1:610186/1‑148 (MQ=255)
                                                                                                                                        aCGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTaa       <  2:1625222/148‑1 (MQ=255)
                                                                                                                                        aCGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTaa       >  1:3228389/1‑148 (MQ=255)
                                                                                                                                        aCGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTaa       >  1:1423006/1‑148 (MQ=255)
                                                                                                                                         cGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAAc      >  2:618452/1‑148 (MQ=255)
                                                                                                                                          gTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACg     <  2:1410813/148‑1 (MQ=255)
                                                                                                                                          gTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACg     >  1:680277/1‑148 (MQ=255)
                                                                                                                                          gTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACg     <  1:2298477/148‑1 (MQ=255)
                                                                                                                                           ttCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGt    >  2:2866216/1‑148 (MQ=255)
                                                                                                                                             ccTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTc  >  2:1210784/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
ACGCACCGATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTC  >  CP009273/477123‑477411

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGCAACGCACCGATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCAGAC  >  CP009273/477119‑477416
                                                                                                                                                    |                                                                                                                                                     
AGCAACGCACCGATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTA                                                                                                                                                      <  SRR19973067.229631/150‑1 (MQ=60)
AGCAACGCACCGATAACCCCCACCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTA                                                                                                                                                      <  SRR19973067.229327/150‑1 (MQ=60)
 GCAACGCACCGATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTAT                                                                                                                                                     <  SRR19973067.3135652/150‑1 (MQ=60)
  CAACGCACCGATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATA                                                                                                                                                    >  SRR19973067.1308321/1‑150 (MQ=60)
   AACGCACCGATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAG                                                                                                                                                   >  SRR19973067.87335/1‑150 (MQ=60)
   AACGCACCGATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAG                                                                                                                                                   >  SRR19973067.87264/1‑150 (MQ=60)
      GCACCGATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGAC                                                                                                                                                >  SRR19973067.2091211/1‑150 (MQ=60)
           GATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCC                                                                                                                                           >  SRR19973067.2754874/1‑150 (MQ=60)
            ATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCC                                                                                                                                          <  SRR19973067.1427887/150‑1 (MQ=60)
             TAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCG                                                                                                                                         <  SRR19973067.222021/150‑1 (MQ=60)
                      GCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCAT                                                                                                                                >  SRR19973067.2437880/1‑150 (MQ=60)
                             GACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAAC                                                                                                                         >  SRR19973067.189801/1‑150 (MQ=60)
                             GACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAAC                                                                                                                         >  SRR19973067.189769/1‑150 (MQ=60)
                              ACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACA                                                                                                                        <  SRR19973067.1902193/150‑1 (MQ=60)
                              ACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACA                                                                                                                        <  SRR19973067.3319718/150‑1 (MQ=60)
                              ACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACA                                                                                                                        <  SRR19973067.3319981/150‑1 (MQ=60)
                                 AGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCG                                                                                                                     <  SRR19973067.94918/150‑1 (MQ=60)
                                  GCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGG                                                                                                                    >  SRR19973067.1611199/1‑150 (MQ=60)
                                     TAACGTAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAG                                                                                                                 <  SRR19973067.1299019/150‑1 (MQ=60)
                                     TAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAG                                                                                                                 <  SRR19973067.2932552/150‑1 (MQ=60)
                                     TAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAG                                                                                                                 >  SRR19973067.1092773/1‑150 (MQ=60)
                                         GGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAG                                                                                                             >  SRR19973067.3224883/1‑150 (MQ=60)
                                          GAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGT                                                                                                            >  SRR19973067.738611/1‑150 (MQ=60)
                                           AGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTT                                                                                                           <  SRR19973067.2255716/150‑1 (MQ=60)
                                           AGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTT                                                                                                           <  SRR19973067.2255722/150‑1 (MQ=60)
                                           AGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTT                                                                                                           <  SRR19973067.1571889/150‑1 (MQ=60)
                                            GAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTT                                                                                                          <  SRR19973067.3533338/150‑1 (MQ=60)
                                             AACGGACTCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCTGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTG                                                                                                         <  SRR19973067.938436/150‑1 (MQ=60)
                                               CGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCT                                                                                                       >  SRR19973067.3083182/1‑150 (MQ=60)
                                                          AGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTT                                                                                            >  SRR19973067.682250/1‑150 (MQ=60)
                                                          AGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTT                                                                                            >  SRR19973067.681996/1‑150 (MQ=60)
                                                              CTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCAT                                                                                        >  SRR19973067.372811/1‑150 (MQ=60)
                                                                CGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCA                                                                                      >  SRR19973067.781580/1‑150 (MQ=60)
                                                                CGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCA                                                                                      <  SRR19973067.841907/150‑1 (MQ=60)
                                                                       CGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCAT                                                                               >  SRR19973067.1673550/1‑150 (MQ=60)
                                                                       CGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCAT                                                                               <  SRR19973067.1226465/150‑1 (MQ=60)
                                                                        GCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATT                                                                              >  SRR19973067.38409/1‑150 (MQ=60)
                                                                        GCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATT                                                                              <  SRR19973067.3458607/150‑1 (MQ=60)
                                                                           GCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCT                                                                           <  SRR19973067.1624686/150‑1 (MQ=60)
                                                                              AGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCA                                                                        <  SRR19973067.1180504/150‑1 (MQ=60)
                                                                                   CAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGT                                                                   <  SRR19973067.2625575/150‑1 (MQ=60)
                                                                                   CAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGT                                                                   <  SRR19973067.1794393/150‑1 (MQ=60)
                                                                                         CACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTT                                                             >  SRR19973067.805689/1‑150 (MQ=60)
                                                                                                    ACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCG                                                  >  SRR19973067.2482927/1‑150 (MQ=60)
                                                                                                     CGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGC                                                 >  SRR19973067.2585774/1‑150 (MQ=60)
                                                                                                     CGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGC                                                 >  SRR19973067.2798749/1‑150 (MQ=60)
                                                                                                     CGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGC                                                 >  SRR19973067.2798782/1‑150 (MQ=60)
                                                                                                       AAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCT                                               >  SRR19973067.2978213/1‑150 (MQ=60)
                                                                                                       AAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCT                                               <  SRR19973067.2249869/150‑1 (MQ=60)
                                                                                                       AAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCT                                               <  SRR19973067.2880903/150‑1 (MQ=60)
                                                                                                       AAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCT                                               >  SRR19973067.1316281/1‑150 (MQ=60)
                                                                                                       AAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCT                                               <  SRR19973067.3437147/150‑1 (MQ=60)
                                                                                                         ATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGG                                             <  SRR19973067.1681032/150‑1 (MQ=60)
                                                                                                         ATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGG                                             <  SRR19973067.742148/150‑1 (MQ=60)
                                                                                                            GCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCT                                          <  SRR19973067.59029/150‑1 (MQ=60)
                                                                                                            GCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCT                                          <  SRR19973067.59107/150‑1 (MQ=60)
                                                                                                             CGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTA                                         <  SRR19973067.2646589/150‑1 (MQ=60)
                                                                                                              GTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAA                                        >  SRR19973067.2452179/1‑150 (MQ=60)
                                                                                                                   GTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTT                                   <  SRR19973067.768551/150‑1 (MQ=60)
                                                                                                                   GTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTT                                   <  SRR19973067.770430/150‑1 (MQ=60)
                                                                                                                     GAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCC                                 <  SRR19973067.621023/150‑1 (MQ=60)
                                                                                                                         GTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGG                             <  SRR19973067.349648/150‑1 (MQ=60)
                                                                                                                           GCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGAT                           <  SRR19973067.2492233/150‑1 (MQ=60)
                                                                                                                           GCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGAT                           <  SRR19973067.59331/150‑1 (MQ=60)
                                                                                                                            CCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATG                          <  SRR19973067.1114076/150‑1 (MQ=60)
                                                                                                                             CTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACAGGTATGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCAGGTGCCGCCTGGCCTAAGATTTCCATGGATGG                         <  SRR19973067.3307400/150‑1 (MQ=60)
                                                                                                                                      GGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTT                >  SRR19973067.873546/1‑150 (MQ=60)
                                                                                                                                        AGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGT              >  SRR19973067.2685539/1‑150 (MQ=60)
                                                                                                                                        AGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGT              >  SRR19973067.2685035/1‑150 (MQ=60)
                                                                                                                                          AGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAA            >  SRR19973067.614247/1‑150 (MQ=60)
                                                                                                                                          AGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAA            >  SRR19973067.614134/1‑150 (MQ=60)
                                                                                                                                          AGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAA            >  SRR19973067.3248655/1‑150 (MQ=60)
                                                                                                                                          AGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAA            >  SRR19973067.1432618/1‑150 (MQ=60)
                                                                                                                                          AGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAA            >  SRR19973067.2508277/1‑150 (MQ=60)
                                                                                                                                           GACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAAC           <  SRR19973067.1001799/150‑1 (MQ=60)
                                                                                                                                            ACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACG          >  SRR19973067.684952/1‑150 (MQ=60)
                                                                                                                                              GTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTT        >  SRR19973067.3405467/1‑150 (MQ=60)
                                                                                                                                              GTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTT        <  SRR19973067.2313637/150‑1 (MQ=60)
                                                                                                                                              GTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTT        >  SRR19973067.2279619/1‑150 (MQ=60)
                                                                                                                                                TCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCC      >  SRR19973067.443274/1‑150 (MQ=60)
                                                                                                                                                    TATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCAGAC  >  SRR19973067.783588/1‑150 (MQ=60)
                                                                                                                                                    TATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCAGAC  <  SRR19973067.535365/150‑1 (MQ=60)
                                                                                                                                                    TATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCAGAC  <  SRR19973067.2611410/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
AGCAACGCACCGATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCAGAC  >  CP009273/477119‑477416

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑