Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 477,267 G→T 100% Q865K (CAG→AAG)  acrB ← multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273477,2670GT95.8% 568.0 / 15.5 190Q865K (CAG→AAG) acrBmultidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base G (2/6);  new base T (90/92);  total (92/98)
Fisher's exact test for biased strand distribution p-value = 2.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 192 total aligned reads displayed.

ACGCACCGATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTC  >  CP009273/477123‑477411
                                                                                                                                                |                                                                                                                                                
aCGCACCGATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTtata                                                                                                                                               <  2:2191763/148‑1 (MQ=255)
 cGCACCGAAACCCCCCAGCGGAACGACCAGAAAAAGGGCGAACGGACTCGACCAGCTCTCGTACAGCGCCGCCANACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAg                                                                                                                                              <  1:1809880/148‑1 (MQ=255)
    aCCGATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGAc                                                                                                                                           <  1:2327031/148‑1 (MQ=255)
     ccGATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACa                                                                                                                                          <  1:131558/148‑1 (MQ=255)
            cccccAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGt                                                                                                                                   <  1:873005/148‑1 (MQ=255)
            cccccAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGt                                                                                                                                   >  1:2353362/1‑148 (MQ=255)
                 aGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGt                                                                                                                              <  2:767342/148‑1 (MQ=255)
                  gCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTc                                                                                                                             <  1:876559/148‑1 (MQ=255)
                   cGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCa                                                                                                                            <  1:363183/148‑1 (MQ=255)
                   cGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCa                                                                                                                            >  2:1479705/1‑148 (MQ=255)
                     gAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGACATCCCCGTCCAGTCATa                                                                                                                          >  2:3387580/1‑148 (MQ=255)
                        cGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGcc                                                                                                                       >  2:973450/1‑148 (MQ=255)
                          aCCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCaa                                                                                                                     >  2:169621/1‑148 (MQ=255)
                            cAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAaca                                                                                                                   <  1:690056/148‑1 (MQ=255)
                            cAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAaca                                                                                                                   >  2:2220788/1‑148 (MQ=255)
                                 tAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGt                                                                                                              <  1:82122/148‑1 (MQ=255)
                                 tAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGt                                                                                                              >  1:2915713/1‑148 (MQ=255)
                                  accGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTa                                                                                                             <  1:399612/146‑1 (MQ=255)
                                  aaCGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTa                                                                                                             >  1:1569620/1‑148 (MQ=255)
                                    cGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAgg                                                                                                           >  2:904853/1‑148 (MQ=255)
                                    cGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAgg                                                                                                           <  2:517905/148‑1 (MQ=255)
                                     ggAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGc                                                                                                          <  1:2200393/148‑1 (MQ=255)
                                     ggAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGc                                                                                                          >  1:2266926/1‑148 (MQ=255)
                                       agaACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAg                                                                                                        <  2:685269/148‑1 (MQ=255)
                                        taaCGGAATCGACCATCTCTCGTACAGCGCCGCCATACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGt                                                                                                       <  2:3242911/147‑1 (MQ=255)
                                        gaACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGt                                                                                                       <  1:3108964/148‑1 (MQ=255)
                                         aaCGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGtt                                                                                                      >  2:1713389/1‑148 (MQ=255)
                                         aaCGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGtt                                                                                                      >  1:495240/1‑148 (MQ=255)
                                          aCGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGttt                                                                                                     >  2:2409711/1‑148 (MQ=255)
                                             gAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCt                                                                                                  <  1:2079933/148‑1 (MQ=255)
                                                tCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGc                                                                                               <  1:3303010/148‑1 (MQ=255)
                                                 cGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGcc                                                                                              >  2:3489912/1‑148 (MQ=255)
                                                 cGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGcc                                                                                              <  1:3009517/148‑1 (MQ=255)
                                                 cGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGcc                                                                                              >  2:3489542/1‑148 (MQ=255)
                                                   aCCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAg                                                                                            >  1:108446/1‑148 (MQ=255)
                                                     cAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAgtt                                                                                          >  2:2090701/1‑148 (MQ=255)
                                                     cAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAgtt                                                                                          >  2:291886/1‑148 (MQ=255)
                                                      aGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAgttg                                                                                         <  1:2910953/148‑1 (MQ=255)
                                                       gCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAgttgt                                                                                        <  2:1732286/148‑1 (MQ=255)
                                                        ctctCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAgttgtt                                                                                       >  1:555299/1‑148 (MQ=255)
                                                        ctctCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAgttgtt                                                                                       >  1:1910850/1‑148 (MQ=255)
                                                        ctctCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAgttgtt                                                                                       >  1:1910475/1‑148 (MQ=255)
                                                         actcGGACAGCCCCCCCAGACACACGACCCCGACCCTCCACGACATCGCGTACAGTGACGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTc                                                                                      <  2:1829047/147‑1 (MQ=255)
                                                           tcGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCa                                                                                    <  1:58403/148‑1 (MQ=255)
                                                           tcGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCa                                                                                    >  1:38114/1‑148 (MQ=255)
                                                           tcGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGCTTGCTCGCCAGTTGTTCCa                                                                                    <  1:1634049/148‑1 (MQ=255)
                                                             gTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATc                                                                                  <  2:1490086/148‑1 (MQ=255)
                                                             gTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATc                                                                                  <  2:232638/148‑1 (MQ=255)
                                                             gTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATc                                                                                  <  1:689271/148‑1 (MQ=255)
                                                                cAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGc                                                                               <  2:286755/148‑1 (MQ=255)
                                                                 aGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCt                                                                              <  1:926465/148‑1 (MQ=255)
                                                                  gcgcCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTc                                                                             <  1:1996293/148‑1 (MQ=255)
                                                                  gcgcCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTc                                                                             <  1:3085165/148‑1 (MQ=255)
                                                                   cgccgcCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTcc                                                                            >  1:460529/1‑148 (MQ=255)
                                                                   cgccgcCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTcc                                                                            >  2:2223648/1‑148 (MQ=255)
                                                                    gccgccAGACACAGGAACACGACACTCAACGAAAGCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCa                                                                           <  2:2575819/148‑1 (MQ=255)
                                                                    gccgccAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCa                                                                           <  2:2222055/148‑1 (MQ=255)
                                                                     ccgccAGACACAGGAACACGCCAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCAt                                                                          <  2:5508/148‑1 (MQ=255)
                                                                       gccAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTg                                                                        <  1:3392925/148‑1 (MQ=255)
                                                                          aGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCtt                                                                     <  1:1225675/148‑1 (MQ=255)
                                                                           gACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTc                                                                    <  1:507549/148‑1 (MQ=255)
                                                                           gACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTc                                                                    >  1:2200942/1‑148 (MQ=255)
                                                                              acaGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCAcc                                                                 >  2:426229/1‑148 (MQ=255)
                                                                               caGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCg                                                                >  1:3235614/1‑148 (MQ=255)
                                                                               caGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCg                                                                <  1:2361526/148‑1 (MQ=255)
                                                                                 ggAACACGACGATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGt                                                              >  1:2532868/1‑148 (MQ=255)
                                                                                  gAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTa                                                             >  1:3065540/1‑148 (MQ=255)
                                                                                    acacGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACt                                                           <  1:1829192/148‑1 (MQ=255)
                                                                                     cacGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACtt                                                          <  1:633479/148‑1 (MQ=255)
                                                                                       cGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACtttt                                                        <  1:1156524/148‑1 (MQ=255)
                                                                                            aTCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCg                                                   >  2:1268649/1‑148 (MQ=255)
                                                                                             tCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCgg                                                  <  1:2223648/148‑1 (MQ=255)
                                                                                             tCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCgg                                                  >  1:298519/1‑148 (MQ=255)
                                                                                             tCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCgg                                                  <  2:2818451/148‑1 (MQ=255)
                                                                                                 cGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTAcc                                                     <  1:2781115/141‑1 (MQ=255)
                                                                                                 cGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTAcc                                                     >  2:2781115/1‑141 (MQ=255)
                                                                                                   aaaTCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTgccgc                                            <  1:169621/148‑1 (MQ=255)
                                                                                                     aTCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCt                                          >  1:257236/1‑148 (MQ=255)
                                                                                                      tCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTg                                         >  1:318763/1‑148 (MQ=255)
                                                                                                      tCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTg                                         >  1:2652255/1‑148 (MQ=255)
                                                                                                       cgcgTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTgg                                        >  2:352210/1‑148 (MQ=255)
                                                                                                         cgTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGcc                                      >  1:1352616/1‑148 (MQ=255)
                                                                                                         cgTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGcc                                      >  1:3495446/1‑148 (MQ=255)
                                                                                                              aGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGa                                 >  1:1073129/1‑148 (MQ=255)
                                                                                                                 gAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGAttt                              <  2:1848459/148‑1 (MQ=255)
                                                                                                                  aaGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTc                             >  1:89039/1‑148 (MQ=255)
                                                                                                                    ggTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCa                           >  2:1783982/1‑148 (MQ=255)
                                                                                                                     gTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCat                          >  1:2672349/1‑148 (MQ=255)
                                                                                                                         cTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCatggat                      >  1:3275127/1‑148 (MQ=255)
                                                                                                                           ggTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCatggatgg                    >  1:3218709/1‑148 (MQ=255)
                                                                                                                           ggTTGCCGGAGAGACGGTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCatggatgg                    <  2:2031995/148‑1 (MQ=255)
                                                                                                                            gTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGc                   >  2:1373294/1‑148 (MQ=255)
                                                                                                                                  ggAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCg             <  2:318763/148‑1 (MQ=255)
                                                                                                                                  ggAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCg             <  2:472804/148‑1 (MQ=255)
                                                                                                                                  ggAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCg             <  2:1889967/148‑1 (MQ=255)
                                                                                                                                   gagagaCGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGt            <  1:2220788/148‑1 (MQ=255)
                                                                                                                                    agagaCGTTCCTGATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGtt           <  1:1073404/148‑1 (MQ=255)
                                                                                                                                       gaCGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTa        >  2:599849/1‑148 (MQ=255)
                                                                                                                                       gaCGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTa        >  2:358521/1‑148 (MQ=255)
                                                                                                                                        aCGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTaa       <  2:108446/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
ACGCACCGATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTC  >  CP009273/477123‑477411

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAGCAACGCACCGATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCA  >  CP009273/477118‑477413
                                                                                                                                                     |                                                                                                                                                  
CAGCAACGCACCGATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTT                                                                                                                                                    <  SRR19973066.645642/150‑1 (MQ=60)
  GCAACGCACCGATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTAT                                                                                                                                                  <  SRR19973066.2300892/150‑1 (MQ=60)
      CGCACCGATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGA                                                                                                                                              >  SRR19973066.2983643/1‑150 (MQ=60)
      CGCACCGAAACCCCCCAGCGGAACGACCAGAAAAAGGGCGAACGGACTCGACCAGCTCTCGTACAGCGCCGCCANACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGA                                                                                                                                              <  SRR19973066.1822740/150‑1 (MQ=60)
        CACCGATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACA                                                                                                                                            <  SRR19973066.320252/150‑1 (MQ=60)
         ACCGATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACAT                                                                                                                                           <  SRR19973066.2342916/150‑1 (MQ=60)
          CCGATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATC                                                                                                                                          <  SRR19973066.132843/150‑1 (MQ=60)
          CCGATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATC                                                                                                                                          <  SRR19973066.133762/150‑1 (MQ=60)
               AACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGT                                                                                                                                     >  SRR19973066.3087820/1‑150 (MQ=60)
               AACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGT                                                                                                                                     >  SRR19973066.2369346/1‑150 (MQ=60)
                 CCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCC                                                                                                                                   <  SRR19973066.879930/150‑1 (MQ=60)
                 CCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCC                                                                                                                                   <  SRR19973066.879249/150‑1 (MQ=60)
                 CCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCC                                                                                                                                   <  SRR19973066.2583680/150‑1 (MQ=60)
                   CCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAG                                                                                                                                 >  SRR19973066.3264301/1‑150 (MQ=60)
                   CCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAG                                                                                                                                 >  SRR19973066.205346/1‑150 (MQ=60)
                       GCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCAT                                                                                                                             <  SRR19973066.882812/150‑1 (MQ=60)
                        CGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATA                                                                                                                            <  SRR19973066.366093/150‑1 (MQ=60)
                        CGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATA                                                                                                                            <  SRR19973066.366032/150‑1 (MQ=60)
                         GGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAG                                                                                                                           <  SRR19973066.385541/150‑1 (MQ=60)
                         GGAACGACCAGCATAACGGAGAACGGAATAGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAG                                                                                                                           >  SRR19973066.440361/1‑150 (MQ=60)
                         GGAACGACCAGCATAACGGAGAACGGAATAGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAG                                                                                                                           >  SRR19973066.1230415/1‑150 (MQ=60)
                               ACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACA                                                                                                                     >  SRR19973066.1587878/1‑150 (MQ=60)
                                 CAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACC                                                                                                                   <  SRR19973066.695132/150‑1 (MQ=60)
                                    CATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGT                                                                                                                >  SRR19973066.2935166/1‑150 (MQ=60)
                                     ATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTA                                                                                                               >  SRR19973066.1580939/1‑150 (MQ=60)
                                      TAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAG                                                                                                              <  SRR19973066.183388/150‑1 (MQ=60)
                                      TAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAG                                                                                                              <  SRR19973066.181481/150‑1 (MQ=60)
                                      TAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAG                                                                                                              <  SRR19973066.83051/150‑1 (MQ=60)
                                       ACCGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGG                                                                                                             <  SRR19973066.402759/150‑1 (MQ=60)
                                        ACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGC                                                                                                            >  SRR19973066.2282561/1‑150 (MQ=60)
                                        ACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGC                                                                                                            >  SRR19973066.2282518/1‑150 (MQ=60)
                                          GGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAG                                                                                                          <  SRR19973066.2215651/150‑1 (MQ=60)
                                          GGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAG                                                                                                          <  SRR19973066.2216455/150‑1 (MQ=60)
                                            AGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTT                                                                                                        >  SRR19973066.498933/1‑150 (MQ=60)
                                             GAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTT                                                                                                       <  SRR19973066.1885455/150‑1 (MQ=60)
                                             GAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTT                                                                                                       <  SRR19973066.3128720/150‑1 (MQ=60)
                                             GAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTT                                                                                                       <  SRR19973066.3129732/150‑1 (MQ=60)
                                              AACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTG                                                                                                      >  SRR19973066.2783534/1‑150 (MQ=60)
                                                  GAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCG                                                                                                  <  SRR19973066.2094296/150‑1 (MQ=60)
                                                     TCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCA                                                                                               <  SRR19973066.3324860/150‑1 (MQ=60)
                                                     TCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCA                                                                                               <  SRR19973066.3324717/150‑1 (MQ=60)
                                                      CGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAG                                                                                              <  SRR19973066.3029551/150‑1 (MQ=60)
                                                      CGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAG                                                                                              >  SRR19973066.109557/1‑150 (MQ=60)
                                                      CGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAG                                                                                              >  SRR19973066.1924713/1‑150 (MQ=60)
                                                          CAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGT                                                                                          >  SRR19973066.2343583/1‑150 (MQ=60)
                                                          CAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGT                                                                                          >  SRR19973066.476387/1‑150 (MQ=60)
                                                           AGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTT                                                                                         >  SRR19973066.1924079/1‑150 (MQ=60)
                                                           AGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTT                                                                                         >  SRR19973066.1924456/1‑150 (MQ=60)
                                                           AGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTT                                                                                         >  SRR19973066.559593/1‑150 (MQ=60)
                                                           AGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTT                                                                                         >  SRR19973066.559282/1‑150 (MQ=60)
                                                           AGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTT                                                                                         <  SRR19973066.2930767/150‑1 (MQ=60)
                                                           AGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTT                                                                                         <  SRR19973066.2930388/150‑1 (MQ=60)
                                                              TCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCA                                                                                      >  SRR19973066.38621/1‑150 (MQ=60)
                                                              TCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCA                                                                                      <  SRR19973066.1266479/150‑1 (MQ=60)
                                                              TCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCA                                                                                      <  SRR19973066.3054025/150‑1 (MQ=60)
                                                                TCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATC                                                                                    <  SRR19973066.3447474/150‑1 (MQ=60)
                                                                TCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATC                                                                                    <  SRR19973066.59129/150‑1 (MQ=60)
                                                                TCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGCTTGCTCGCCAGTTGTTCCATC                                                                                    <  SRR19973066.1645929/150‑1 (MQ=60)
                                                                 agtgagtgatccccaatgtggcacaacgatcttctatCGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCA                                                                                   <  SRR19973066.1086654/113‑1 (MQ=60)
                                                                  GTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAG                                                                                  <  SRR19973066.694343/150‑1 (MQ=60)
                                                                  GTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAG                                                                                  <  SRR19973066.694801/150‑1 (MQ=60)
                                                                      AGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCC                                                                              >  SRR19973066.464052/1‑150 (MQ=60)
                                                                      AGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCC                                                                              <  SRR19973066.933168/150‑1 (MQ=60)
                                                                      AGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCC                                                                              <  SRR19973066.933614/150‑1 (MQ=60)
                                                                      AGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCC                                                                              >  SRR19973066.2268837/1‑150 (MQ=60)
                                                                       GCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCA                                                                             <  SRR19973066.2010333/150‑1 (MQ=60)
                                                                       GCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCA                                                                             <  SRR19973066.3105780/150‑1 (MQ=60)
                                                                         GCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATT                                                                           <  SRR19973066.690553/150‑1 (MQ=60)
                                                                            GCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCT                                                                        <  SRR19973066.3415567/150‑1 (MQ=60)
                                                                              CAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTC                                                                      >  SRR19973066.2216198/1‑150 (MQ=60)
                                                                              CAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTC                                                                      >  SRR19973066.2216203/1‑150 (MQ=60)
                                                                              CAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTC                                                                      >  SRR19973066.716146/1‑150 (MQ=60)
                                                                               AGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCA                                                                     <  SRR19973066.1234354/150‑1 (MQ=60)
                                                                                GACACAGGAACACGACAATCAACGAAATCGCGTACAGTGTAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCAC                                                                    <  SRR19973066.511290/150‑1 (MQ=60)
                                                                                GACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCAC                                                                    <  SRR19973066.511314/150‑1 (MQ=60)
                                                                                  CACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCG                                                                  >  SRR19973066.3256977/1‑150 (MQ=60)
                                                                                  CACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCG                                                                  <  SRR19973066.2377732/150‑1 (MQ=60)
                                                                                    CAGGAACACGACGATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGT                                                                >  SRR19973066.2550088/1‑150 (MQ=60)
                                                                                    CAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGT                                                                >  SRR19973066.3027538/1‑150 (MQ=60)
                                                                                    CAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGT                                                                <  SRR19973066.2377532/150‑1 (MQ=60)
                                                                                     AGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTA                                                               >  SRR19973066.3086042/1‑150 (MQ=60)
                                                                                         ACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTT                                                           <  SRR19973066.1842252/150‑1 (MQ=60)
                                                                                          CACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTT                                                          <  SRR19973066.638038/150‑1 (MQ=60)
                                                                                            CGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTAC                                                        <  SRR19973066.1164747/150‑1 (MQ=60)
                                                                                               gatctAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCG                                                     <  SRR19973066.2799842/145‑1 (MQ=60)
                                                                                               CAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCG                                                     <  SRR19973066.523813/150‑1 (MQ=60)
                                                                                                AATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGG                                                    >  SRR19973066.300629/1‑150 (MQ=60)
                                                                                                AATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGGACTTTTACCCGG                                                    >  SRR19973066.2111233/1‑150 (MQ=60)
                                                                                                  TCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTG                                                  <  SRR19973066.2239018/150‑1 (MQ=60)
                                                                                                     ACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCG                                               <  SRR19973066.1497057/150‑1 (MQ=60)
                                                                                                        AAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCT                                            >  SRR19973066.259183/1‑150 (MQ=60)
                                                                                                        AAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCT                                            <  SRR19973066.171142/150‑1 (MQ=60)
                                                                                                        AAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCT                                            >  SRR19973066.3567764/1‑150 (MQ=60)
                                                                                                         AATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTG                                           >  SRR19973066.321153/1‑150 (MQ=60)
                                                                                                         AATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTG                                           >  SRR19973066.871474/1‑150 (MQ=60)
                                                                                                         AATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTG                                           >  SRR19973066.2671134/1‑150 (MQ=60)
                                                                                                         AATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTG                                           >  SRR19973066.2670055/1‑150 (MQ=60)
                                                                                                            CGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCC                                        >  SRR19973066.1362413/1‑150 (MQ=60)
                                                                                                            CGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCC                                        >  SRR19973066.3518751/1‑150 (MQ=60)
                                                                                                              CGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTA                                      >  SRR19973066.884317/1‑150 (MQ=60)
                                                                                                                 ACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGA                                   >  SRR19973066.1080991/1‑150 (MQ=60)
                                                                                                                   AGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATT                                 >  SRR19973066.3229909/1‑150 (MQ=60)
                                                                                                                     TGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTC                               >  SRR19973066.90009/1‑150 (MQ=60)
                                                                                                                        AGGTGCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCAT                            >  SRR19973066.2690205/1‑150 (MQ=60)
                                                                                                                            GCCTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGAT                        >  SRR19973066.3296618/1‑150 (MQ=60)
                                                                                                                              CTGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGG                      >  SRR19973066.3240009/1‑150 (MQ=60)
                                                                                                                               TGGTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGC                     >  SRR19973066.168805/1‑150 (MQ=60)
                                                                                                                                 GTTGCCGGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAG                   >  SRR19973066.927072/1‑150 (MQ=60)
                                                                                                                                     CCGGAGAGACGTTCCTTATAGGACATCCCCGTACAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATAAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCG               >  SRR19973066.2877129/1‑150 (MQ=60)
                                                                                                                                       GGAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTT             <  SRR19973066.1129673/150‑1 (MQ=60)
                                                                                                                                        GAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTG            <  SRR19973066.2236369/150‑1 (MQ=60)
                                                                                                                                        GAGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTG            <  SRR19973066.2236149/150‑1 (MQ=60)
                                                                                                                                         AGAGACGTTCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGT           >  SRR19973066.2375233/1‑150 (MQ=60)
                                                                                                                                         AGAGACGTTCCTGATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGT           <  SRR19973066.1081316/150‑1 (MQ=60)
                                                                                                                                         AGAGACGTTCCTGATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGT           <  SRR19973066.1081266/150‑1 (MQ=60)
                                                                                                                                                 TCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCC   >  SRR19973066.2892557/1‑150 (MQ=60)
                                                                                                                                                 TCCTTATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCC   >  SRR19973066.2892560/1‑150 (MQ=60)
                                                                                                                                                  CCTGATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCA  <  SRR19973066.3410186/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                  
CAGCAACGCACCGATAACCCCCAGCGGAACGACCAGCATAACGGAGAACGGAATCGACCAGCTCTCGTACAGCGCCGCCAGACACAGGAACACGACAATCAACGAAATCGCGTACAGTGAAGGTGCCTGGTTGCCGGAGAGACGTTCCTGATAGGACATCCCCGTCCAGTCATAGCCAACACCGGTAGGCAGTTTGCTCGCCAGTTGTTCCATCAGCTCCATTGCTTCACCGGTACTTTTACCCGGTGCCGCCTGGCCTAAGATTTCCATGGATGGCAGGCCGTTGTAACGTTCCA  >  CP009273/477118‑477413

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑