Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 517,392 G→T 100% A507A (GCG→GCT)  ybbP → putative ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273517,3920GT96.2% 504.7 / 10.0 167A507A (GCG→GCT) ybbPputative ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (1/6);  new base T (75/85);  total (76/91)
Fisher's exact test for biased strand distribution p-value = 1.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 169 total aligned reads displayed.

CATTTTCGCTCTCCTTTATGCTGCTGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCGTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGCAAAATACGCGG  >  CP009273/517247‑517536
                                                                                                                                                 |                                                                                                                                                
cATTTTCGCTCTCCTTTATGCTGCTGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCttt                                                                                                                                                <  1:1698509/148‑1 (MQ=255)
   tttCGCTCTCCTTTATGCTGCTGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCt                                                                                                                                             <  1:293568/148‑1 (MQ=255)
   tttCGCTCTCCTTTATGCTGCTGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCt                                                                                                                                             >  1:820539/1‑148 (MQ=255)
   tttCGCTCTCCTTTATGCTGCTGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCt                                                                                                                                             <  1:1463767/148‑1 (MQ=255)
    ttCGCTCTCCTTTATGCTGCTGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTc                                                                                                                                            >  1:2887785/1‑148 (MQ=255)
        ctctCCTTTATGCTGCTGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCgg                                                                                                                                        >  1:3385556/1‑148 (MQ=255)
        ctctCCTTTATGCTGCTGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCgg                                                                                                                                        >  1:2789979/1‑148 (MQ=255)
        ctctCCTTTATGCTGCTGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCgg                                                                                                                                        >  1:1272815/1‑148 (MQ=255)
         tctcCTTTATGCTGCTGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTCAAAGCTTTCCTCGCGGa                                                                                                                                       >  1:723348/1‑148 (MQ=255)
              tttATGCTGCTGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATc                                                                                                                                  <  1:2643095/148‑1 (MQ=255)
                 aTGCTGCTGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGa                                                                                                                               <  2:188035/148‑1 (MQ=255)
                    ctgctgGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAg                                                                                                                            >  2:2548010/1‑148 (MQ=255)
                     tgctgGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGt                                                                                                                           <  2:1430786/148‑1 (MQ=255)
                      gctgGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTc                                                                                                                          <  2:1461360/148‑1 (MQ=255)
                         ggCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCg                                                                                                                       >  2:3365841/1‑148 (MQ=255)
                          gCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCgg                                                                                                                      >  2:3460362/1‑148 (MQ=255)
                          gCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCgg                                                                                                                      >  2:1137253/1‑148 (MQ=255)
                           cACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGa                                                                                                                     <  1:12857/148‑1 (MQ=255)
                            aCTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGaa                                                                                                                    <  2:734093/148‑1 (MQ=255)
                              tgctgctgGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATc                                                                                                                  >  2:1520008/1‑148 (MQ=255)
                               gctgctgGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCg                                                                                                                 <  2:164775/148‑1 (MQ=255)
                                ctgctgGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGt                                                                                                                <  1:2288958/148‑1 (MQ=255)
                                  gctgGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGttt                                                                                                              <  2:110309/148‑1 (MQ=255)
                                  gctgGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGttt                                                                                                              >  1:2736240/1‑148 (MQ=255)
                                    tgGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTa                                                                                                            <  2:725589/148‑1 (MQ=255)
                                      gtgtTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATc                                                                                                          <  1:1387076/148‑1 (MQ=255)
                                       tgtTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATcc                                                                                                         >  1:1060028/1‑148 (MQ=255)
                                         ttGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCgg                                                                                                       <  2:3012397/148‑1 (MQ=255)
                                          tGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCggt                                                                                                      <  1:1158219/148‑1 (MQ=255)
                                          tGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCggt                                                                                                      >  2:237652/1‑148 (MQ=255)
                                          tGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCggt                                                                                                      >  2:525845/1‑148 (MQ=255)
                                          tGCGTGGCGATCTGCTCGACCGCTGGCAGAAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCAACAGAACAGTTACCAACGCTAAAAGCTTTCCTCGCGCAACATCAGATAGTCCCGCAATCGTTTTATCCggt                                                                                                      >  2:525956/1‑148 (MQ=255)
                                           gCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCggtg                                                                                                     <  2:417904/148‑1 (MQ=255)
                                            cGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCggtgg                                                                                                    <  1:79111/148‑1 (MQ=255)
                                                  gATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCggg                                                                                              <  2:2863409/148‑1 (MQ=255)
                                                      tGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGcgcg                                                                                          <  2:3444718/148‑1 (MQ=255)
                                                       gCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCgg                                                                                         <  2:3301471/148‑1 (MQ=255)
                                                       gCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCgg                                                                                         <  2:135161/148‑1 (MQ=255)
                                                          cGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTg                                                                                      <  1:359021/148‑1 (MQ=255)
                                                                cTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCggtggt                                                                                                   >  2:1900082/1‑129 (MQ=255)
                                                                cTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCggtggt                                                                                                   >  2:1899616/1‑129 (MQ=255)
                                                                cTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCggtggt                                                                                                   <  1:1899616/129‑1 (MQ=255)
                                                                 tGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGa                                                                               >  1:2452369/1‑148 (MQ=255)
                                                                 tGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCGTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGa                                                                               <  1:463013/148‑1 (MQ=255)
                                                                    cagcagcagCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTa                                                                            <  1:1038823/148‑1 (MQ=255)
                                                                    cagcagcagCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTa                                                                            <  2:126564/148‑1 (MQ=255)
                                                                     agcagcagcTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTaa                                                                           <  1:591707/148‑1 (MQ=255)
                                                                     agcagcagcTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTaa                                                                           <  1:1978578/148‑1 (MQ=255)
                                                                       cagcagcTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACg                                                                         <  2:1218120/148‑1 (MQ=255)
                                                                         gcagcTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGAt                                                                       <  1:1520008/148‑1 (MQ=255)
                                                                          cagcTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATa                                                                      >  2:2413341/1‑148 (MQ=255)
                                                                          cagcTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATa                                                                      <  1:1237088/148‑1 (MQ=255)
                                                                               aCCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCg                                                                 >  1:293838/1‑148 (MQ=255)
                                                                               aCCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCg                                                                 <  1:2166847/148‑1 (MQ=255)
                                                                                 cTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGAc                                                               <  1:1884597/148‑1 (MQ=255)
                                                                                    cAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTCGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGTGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGa                                                            <  2:891555/148‑1 (MQ=255)
                                                                                     aGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGaa                                                           >  1:2866958/1‑148 (MQ=255)
                                                                                        aaGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGt                                                        <  1:1996082/148‑1 (MQ=255)
                                                                                         aGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTa                                                       >  2:765572/1‑148 (MQ=255)
                                                                                          gCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTaa                                                      <  2:1272815/148‑1 (MQ=255)
                                                                                          gCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTaa                                                      >  1:2868188/1‑148 (MQ=255)
                                                                                          gCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTaa                                                      >  2:2289263/1‑148 (MQ=255)
                                                                                           cccGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAAt                                                     >  1:1195807/1‑148 (MQ=255)
                                                                                           cccGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCGTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAAt                                                     <  1:1550824/148‑1 (MQ=255)
                                                                                            ccGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATg                                                    <  1:3058995/148‑1 (MQ=255)
                                                                                              gAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGaa                                                  >  1:1226551/1‑148 (MQ=255)
                                                                                                actactTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGa                                                >  2:1185836/1‑148 (MQ=255)
                                                                                                 ctactTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGAt                                               >  1:668435/1‑148 (MQ=255)
                                                                                                  tactTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATg                                              >  2:2183457/1‑148 (MQ=255)
                                                                                                   actTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGa                                             <  2:1600814/148‑1 (MQ=255)
                                                                                                   actTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGa                                             <  2:2664909/148‑1 (MQ=255)
                                                                                                     tttttAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAgg                                           <  1:2591959/148‑1 (MQ=255)
                                                                                                        ttaattaaCATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGgcgc                                        <  2:342068/148‑1 (MQ=255)
                                                                                                           attaaCATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTa                                     <  1:3363581/148‑1 (MQ=255)
                                                                                                           attaaCATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTa                                     <  1:3364768/148‑1 (MQ=255)
                                                                                                             taaCATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAAc                                   <  2:2650253/148‑1 (MQ=255)
                                                                                                                cATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACcgc                                >  2:2927815/1‑148 (MQ=255)
                                                                                                                 aTCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACcgcg                               >  2:2805240/1‑148 (MQ=255)
                                                                                                                        caGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGCTAAGCCGACAGACGGTCATGAAGAGGAGGCGCTGACCCGCGACCTcta                        >  2:3393641/1‑146 (MQ=255)
                                                                                                                         aGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAAt                       >  1:3139122/1‑148 (MQ=255)
                                                                                                                           aaCAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCt                     >  2:2077472/1‑148 (MQ=255)
                                                                                                                           aaCAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCt                     >  2:619906/1‑148 (MQ=255)
                                                                                                                           aaCAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCt                     <  2:3284899/148‑1 (MQ=255)
                                                                                                                            aCAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCtt                    <  1:2413341/148‑1 (MQ=255)
                                                                                                                             cAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTa                   <  2:2156222/148‑1 (MQ=255)
                                                                                                                             cAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTa                   <  2:820539/148‑1 (MQ=255)
                                                                                                                               ggTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTAcc                 <  1:385198/148‑1 (MQ=255)
                                                                                                                                 tAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTg               >  2:276342/1‑148 (MQ=255)
                                                                                                                                    cACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGCa            >  2:3552211/1‑148 (MQ=255)
                                                                                                                                     aCCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGCaa           >  1:996185/1‑148 (MQ=255)
                                                                                                                                      ccGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGCaaa          <  2:3479060/148‑1 (MQ=255)
                                                                                                                                        gCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGCAAAAt        <  1:2761318/148‑1 (MQ=255)
                                                                                                                                        gCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGCAAAAt        <  1:3158525/148‑1 (MQ=255)
                                                                                                                                          tAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGCAAAATAc      >  2:3123391/1‑148 (MQ=255)
                                                                                                                                           aaaaGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAAAGATAAGACGACAGAAGGTAAAGAAGATGAGTCGCTTAACCGCGAACTCAATCTTACCTGGCAAAATAcg     >  1:3518411/1‑148 (MQ=255)
                                                                                                                                            aaaGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGCAAAATAcgc    >  1:1625746/1‑148 (MQ=255)
                                                                                                                                            aaaGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGCAAAATAcgc    <  2:2452369/148‑1 (MQ=255)
                                                                                                                                             aaGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGCAAAATAcgcg   >  1:2238464/1‑148 (MQ=255)
                                                                                                                                             aaGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGCAAAATAcgcg   >  1:1999198/1‑148 (MQ=255)
                                                                                                                                              aGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGCAAAATACGCgg  >  1:2961494/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CATTTTCGCTCTCCTTTATGCTGCTGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCGTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGCAAAATACGCGG  >  CP009273/517247‑517536

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCGGCATTTTCGCTCTCCTTTATGCTGCTGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCGTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGCAAAATACGCGGCCCGA  >  CP009273/517243‑517541
                                                                                                                                                     |                                                                                                                                                     
TCGGCATTTTCGCTCTCCTTTATGCTGCTGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCT                                                                                                                                                       >  SRR19973067.3194532/1‑150 (MQ=60)
TCGGCATTTTCGCTCTCCTTTATGCTGCTGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCT                                                                                                                                                       >  SRR19973067.127729/1‑150 (MQ=60)
   GCATTTTCGCTCTCCTTTATGCTGCTGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTC                                                                                                                                                    >  SRR19973067.2651884/1‑150 (MQ=60)
   GCATTTTCGCTCTCCTTTATGCTGCTGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTC                                                                                                                                                    <  SRR19973067.3088659/150‑1 (MQ=60)
    CATTTTCGCTCTCCTTTATGCTGCTGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCC                                                                                                                                                   <  SRR19973067.3481935/150‑1 (MQ=60)
    CATTTTCGCTCTCCTTTATGCTGCTGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCC                                                                                                                                                   <  SRR19973067.1710188/150‑1 (MQ=60)
     ATTTTCGCTCTCCTTTATGCTGCTGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCT                                                                                                                                                  >  SRR19973067.826154/1‑150 (MQ=60)
     ATTTTCGCTCTCCTTTATGCTGCTGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCT                                                                                                                                                  >  SRR19973067.2170643/1‑150 (MQ=60)
      TTTTCGCTCTCCTTTATGCTGCTGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTC                                                                                                                                                 >  SRR19973067.2906243/1‑150 (MQ=60)
       TTTCGCTCTCCTTTATGCTGCTGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCG                                                                                                                                                <  SRR19973067.295452/150‑1 (MQ=60)
       TTTCGCTCTCCTTTATGCTGCTGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCG                                                                                                                                                <  SRR19973067.1473543/150‑1 (MQ=60)
          CGCTCTCCTTTATGCTGCTGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGG                                                                                                                                             >  SRR19973067.2807979/1‑150 (MQ=60)
          CGCTCTCCTTTATGCTGCTGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGG                                                                                                                                             >  SRR19973067.1281354/1‑150 (MQ=60)
          CGCTCTCCTTTATGCTGCTGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGG                                                                                                                                             >  SRR19973067.1281473/1‑150 (MQ=60)
          CGCTCTCCTTTATGCTGCTGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGG                                                                                                                                             >  SRR19973067.3407069/1‑150 (MQ=60)
           GCTCTCCTTTATGCTGCTGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTCAAAGCTTTCCTCGCGGA                                                                                                                                            >  SRR19973067.728373/1‑150 (MQ=60)
            CTCTCCTTTATGCTGCTGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAA                                                                                                                                           >  SRR19973067.2897329/1‑150 (MQ=60)
             TCTCCTTTATGCTGCTGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAAC                                                                                                                                          <  SRR19973067.1450166/150‑1 (MQ=60)
                  TTTATGCTGCTGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAG                                                                                                                                     <  SRR19973067.2660167/150‑1 (MQ=60)
                           CTGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCG                                                                                                                            <  SRR19973067.2790514/150‑1 (MQ=60)
                            TGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGG                                                                                                                           >  SRR19973067.1087913/1‑150 (MQ=60)
                               CACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAAT                                                                                                                        <  SRR19973067.13059/150‑1 (MQ=60)
                                    CTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTT                                                                                                                   <  SRR19973067.1549342/150‑1 (MQ=60)
                                    CTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTT                                                                                                                   >  SRR19973067.2753906/1‑150 (MQ=60)
                                    CTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTT                                                                                                                   >  SRR19973067.2754450/1‑150 (MQ=60)
                                    CTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTT                                                                                                                   <  SRR19973067.2304085/150‑1 (MQ=60)
                                         GGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGACTCGTGTTATCC                                                                                                              >  SRR19973067.2853556/1‑150 (MQ=60)
                                         GGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCC                                                                                                              >  SRR19973067.1067296/1‑150 (MQ=60)
                                          GTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCG                                                                                                             <  SRR19973067.1396512/150‑1 (MQ=60)
                                          GTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCG                                                                                                             <  SRR19973067.1395920/150‑1 (MQ=60)
                                             TTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTG                                                                                                          <  SRR19973067.376321/150‑1 (MQ=60)
                                              TGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGG                                                                                                         <  SRR19973067.2948932/150‑1 (MQ=60)
                                              TGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGG                                                                                                         <  SRR19973067.1165937/150‑1 (MQ=60)
                                                CGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTG                                                                                                       <  SRR19973067.79990/150‑1 (MQ=60)
                                                CGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTG                                                                                                       <  SRR19973067.79977/150‑1 (MQ=60)
                                                 gtgtgctcttccgatctCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGC                                                                                                      <  SRR19973067.1913041/133‑1 (MQ=60)
                                                 gtgtgctcttccgatctCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGC                                                                                                      <  SRR19973067.1912573/133‑1 (MQ=60)
                                                 GTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGC                                                                                                      >  SRR19973067.2372513/1‑150 (MQ=60)
                                                           GCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCT                                                                                            <  SRR19973067.702163/150‑1 (MQ=60)
                                                              CGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGAC                                                                                         <  SRR19973067.361513/150‑1 (MQ=60)
                                                                   GCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGA                                                                                    >  SRR19973067.2468489/1‑150 (MQ=60)
                                                                     TGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCGTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATT                                                                                  <  SRR19973067.466217/150‑1 (MQ=60)
                                                                     TGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCGTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATT                                                                                  <  SRR19973067.465968/150‑1 (MQ=60)
                                                                        CAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAAC                                                                               <  SRR19973067.1045970/150‑1 (MQ=60)
                                                                         AGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACG                                                                              <  SRR19973067.3001667/150‑1 (MQ=60)
                                                                         AGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACG                                                                              <  SRR19973067.1991978/150‑1 (MQ=60)
                                                                         AGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACG                                                                              <  SRR19973067.595470/150‑1 (MQ=60)
                                                                             GCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAA                                                                          <  SRR19973067.3300948/150‑1 (MQ=60)
                                                                             GCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAA                                                                          <  SRR19973067.1530173/150‑1 (MQ=60)
                                                                              CAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAG                                                                         <  SRR19973067.1245391/150‑1 (MQ=60)
                                                                              CAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAG                                                                         <  SRR19973067.1244237/150‑1 (MQ=60)
                                                                                 CTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCG                                                                      >  SRR19973067.295722/1‑150 (MQ=60)
                                                                                 CTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCG                                                                      >  SRR19973067.297748/1‑150 (MQ=60)
                                                                                   ACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGAC                                                                    <  SRR19973067.2181337/150‑1 (MQ=60)
                                                                                     CTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAG                                                                  <  SRR19973067.1897464/150‑1 (MQ=60)
                                                                                       CCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAA                                                                >  SRR19973067.2885320/1‑150 (MQ=60)
                                                                                            AAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAA                                                           >  SRR19973067.2886553/1‑150 (MQ=60)
                                                                                            AAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAA                                                           <  SRR19973067.2009561/150‑1 (MQ=60)
                                                                                             AGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAAT                                                          >  SRR19973067.1203646/1‑150 (MQ=60)
                                                                                               CCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCGTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGA                                                        <  SRR19973067.1561385/150‑1 (MQ=60)
                                                                                                CCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAA                                                       >  SRR19973067.1234747/1‑150 (MQ=60)
                                                                                                CCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAA                                                       <  SRR19973067.3078470/150‑1 (MQ=60)
                                                                                                   AACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGAT                                                    >  SRR19973067.673011/1‑150 (MQ=60)
                                                                                                       ACTTTTTAATTAACCTCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGG                                                <  SRR19973067.187654/150‑1 (MQ=60)
                                                                                                         TTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCG                                              >  SRR19973067.247851/1‑150 (MQ=60)
                                                                                                         TTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCG                                              <  SRR19973067.2608829/150‑1 (MQ=60)
                                                                                                           TTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCGTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCT                                            <  SRR19973067.536433/150‑1 (MQ=60)
                                                                                                               ATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAAC                                        <  SRR19973067.3386125/150‑1 (MQ=60)
                                                                                                               ATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAAC                                        <  SRR19973067.3384931/150‑1 (MQ=60)
                                                                                                                       CGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACT                                <  SRR19973067.3387206/150‑1 (MQ=60)
                                                                                                                           ACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAAT                            <  SRR19973067.1604032/150‑1 (MQ=60)
                                                                                                                           ACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAAT                            >  SRR19973067.3159100/1‑150 (MQ=60)
                                                                                                                           ACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAAT                            >  SRR19973067.3159056/1‑150 (MQ=60)
                                                                                                                           ACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAAT                            <  SRR19973067.2264142/150‑1 (MQ=60)
                                                                                                                             AGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCT                          <  SRR19973067.1911895/150‑1 (MQ=60)
                                                                                                                                ACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTAC                       <  SRR19973067.2429138/150‑1 (MQ=60)
                                                                                                                                   GGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTG                    >  SRR19973067.1182066/1‑150 (MQ=60)
                                                                                                                                   GGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTG                    <  SRR19973067.387884/150‑1 (MQ=60)
                                                                                                                                       GCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGCAA                >  SRR19973067.1003040/1‑150 (MQ=60)
                                                                                                                                            GCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGCAAAATAC           <  SRR19973067.3178540/150‑1 (MQ=60)
                                                                                                                                            GCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGCAAAATAC           <  SRR19973067.2779162/150‑1 (MQ=60)
                                                                                                                                             CTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGCAAAATACG          >  SRR19973067.901744/1‑150 (MQ=60)
                                                                                                                                             CTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAAAGATAAGACGACAGAAGGTAAAGAAGATGAGTCGCTTAACCGCGAACTCAATCTTACCTGGCAAAATACG          >  SRR19973067.3540676/1‑150 (MQ=60)
                                                                                                                                              TAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTAAATCTTACCTGGCAAAATACGC         >  SRR19973067.1636897/1‑150 (MQ=60)
                                                                                                                                               AAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGCAAAATACGCG        >  SRR19973067.2012507/1‑150 (MQ=60)
                                                                                                                                               AAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGCAAAATACGCG        >  SRR19973067.2012689/1‑150 (MQ=60)
                                                                                                                                               AAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGCAAAATACGCG        >  SRR19973067.2253355/1‑150 (MQ=60)
                                                                                                                                                AAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGCAAAATACGCGG       >  SRR19973067.2980201/1‑150 (MQ=60)
                                                                                                                                                    CTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAATCCGACAGAAGGTAATTAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGCAAAATACGCGGCCCG   >  SRR19973067.2946255/1‑150 (MQ=60)
                                                                                                                                                     TTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGCAAAATACGCGGCCCGA  >  SRR19973067.3023235/1‑150 (MQ=60)
                                                                                                                                                     TTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTAACCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGCAAAAAACGCGGCCCGA  >  SRR19973067.3023198/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
TCGGCATTTTCGCTCTCCTTTATGCTGCTGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCGTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGCAAAATACGCGGCCCGA  >  CP009273/517243‑517541

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑