Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 517,392 G→T 100% A507A (GCG→GCT)  ybbP → putative ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273517,3920GT97.3% 464.5 / 3.8 150A507A (GCG→GCT) ybbPputative ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base G (1/3);  new base T (76/70);  total (77/73)
Fisher's exact test for biased strand distribution p-value = 3.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 151 total aligned reads displayed.

CATTTTCGCTCTCCTTTATGCTGCTGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCGTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGCAAAATACGCGG  >  CP009273/517247‑517536
                                                                                                                                                 |                                                                                                                                                
cATTTTCGCTCTCCTTTATGCTGCTGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCttt                                                                                                                                                <  2:2617125/148‑1 (MQ=255)
  ttttCGCTCTCCTTTATGCTGCTGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTcc                                                                                                                                              >  1:1679133/1‑148 (MQ=255)
  ttttCGCTCTCCTTTATGCTGCTGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTcc                                                                                                                                              >  2:3043885/1‑148 (MQ=255)
   tttCGCTCTCCTTTATGCTGCTGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCt                                                                                                                                             >  1:1100922/1‑148 (MQ=255)
   tttCGCTCTCCTTTATGCTGCTGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCt                                                                                                                                             >  1:834799/1‑148 (MQ=255)
          ctcCTTTATGCTGCTGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGaa                                                                                                                                      >  1:2376546/1‑148 (MQ=255)
          ctcCTTTATGCTGCTGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGaa                                                                                                                                      >  2:2273406/1‑148 (MQ=255)
          ctcCTTTATGCTGCTGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGaa                                                                                                                                      >  2:481442/1‑148 (MQ=255)
           tcCTTTATGCTGCTGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAAc                                                                                                                                     >  2:2543924/1‑148 (MQ=255)
           tcCTTTATGCTGCTGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAAc                                                                                                                                     >  1:2849076/1‑148 (MQ=255)
            ccTTTATGCTGCTGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACa                                                                                                                                    >  2:868878/1‑148 (MQ=255)
            ccTTTATGCTGCTGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACa                                                                                                                                    >  1:115742/1‑148 (MQ=255)
             cTTTATGCTGCTGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACAt                                                                                                                                   <  1:2909474/148‑1 (MQ=255)
              tttATGCTGCTGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCTCTAAAAGCTTTCCTCGCGGAACATc                                                                                                                                  <  1:1457902/148‑1 (MQ=255)
                tATGCTGCTGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAg                                                                                                                                <  2:309930/148‑1 (MQ=255)
                 aTGCTGCTGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGa                                                                                                                               <  1:881773/148‑1 (MQ=255)
                   gctgctGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATa                                                                                                                             >  2:1257410/1‑148 (MQ=255)
                   gctgctGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATa                                                                                                                             >  2:1257393/1‑148 (MQ=255)
                    ctgctgGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAg                                                                                                                            <  1:1421636/148‑1 (MQ=255)
                          gCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCgg                                                                                                                      >  1:1918576/1‑148 (MQ=255)
                            aCTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGaa                                                                                                                    <  1:992663/148‑1 (MQ=255)
                             ctgctgctgGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAAt                                                                                                                   >  2:1651781/1‑148 (MQ=255)
                             ctgctgctgGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAAt                                                                                                                   >  1:617158/1‑148 (MQ=255)
                               gctgctgGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCg                                                                                                                 <  1:1748787/148‑1 (MQ=255)
                                 tgctgGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGtt                                                                                                               >  2:1497141/1‑148 (MQ=255)
                                   ctgGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGtttt                                                                                                             >  2:908202/1‑148 (MQ=255)
                                   ctgGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGtttt                                                                                                             <  1:2381253/148‑1 (MQ=255)
                                   ctgGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGtttt                                                                                                             <  1:1772873/148‑1 (MQ=255)
                                     ggTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTAt                                                                                                           >  2:914015/1‑148 (MQ=255)
                                     ggTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTAt                                                                                                           <  1:1168185/148‑1 (MQ=255)
                                      gtgtTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATc                                                                                                          <  1:394272/148‑1 (MQ=255)
                                          tGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCggt                                                                                                      <  2:765581/148‑1 (MQ=255)
                                          tGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCggt                                                                                                      <  2:2980080/148‑1 (MQ=255)
                                                gCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACACAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCg                                                                                                >  1:249495/1‑148 (MQ=255)
                                                   aTCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGgc                                                                                             >  1:552128/1‑148 (MQ=255)
                                                    tCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGgcg                                                                                            >  1:817215/1‑148 (MQ=255)
                                                       gCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCgg                                                                                         <  2:1807312/148‑1 (MQ=255)
                                                        cTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGc                                                                                        >  1:222391/1‑148 (MQ=255)
                                                         tCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCt                                                                                       <  1:1817205/148‑1 (MQ=255)
                                                          cGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTg                                                                                      >  2:2454169/1‑148 (MQ=255)
                                                            aCCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGAc                                                                                    >  2:317288/1‑148 (MQ=255)
                                                                 tGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGa                                                                               <  2:115742/148‑1 (MQ=255)
                                                                    cagcagcagCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTa                                                                            <  2:713530/148‑1 (MQ=255)
                                                                    cagcagcagCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTa                                                                            <  2:713388/148‑1 (MQ=255)
                                                                      gcagcagcTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAAc                                                                          >  1:2757793/1‑148 (MQ=255)
                                                                       cagcagcTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACg                                                                         <  1:1402909/148‑1 (MQ=255)
                                                                       cagcagcTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACg                                                                         >  1:2977495/1‑148 (MQ=255)
                                                                       cagcagcTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACg                                                                         >  1:2973266/1‑148 (MQ=255)
                                                                       cagcagcTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACg                                                                         <  1:2234368/148‑1 (MQ=255)
                                                                          cagcTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATa                                                                      >  2:2093524/1‑148 (MQ=255)
                                                                          cagcTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATa                                                                      >  1:3076883/1‑148 (MQ=255)
                                                                            gcTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAg                                                                    <  2:1391470/148‑1 (MQ=255)
                                                                            gcTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAg                                                                    <  2:2646860/148‑1 (MQ=255)
                                                                                ccTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGa                                                                <  2:193207/148‑1 (MQ=255)
                                                                                  tCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACa                                                              <  1:2756838/148‑1 (MQ=255)
                                                                                    cAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGa                                                            >  2:1996278/1‑148 (MQ=255)
                                                                                     aGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGaa                                                           >  2:2612358/1‑148 (MQ=255)
                                                                                         aGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTa                                                       >  2:2472490/1‑148 (MQ=255)
                                                                                         aGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTa                                                       <  2:2771648/148‑1 (MQ=255)
                                                                                         aGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTa                                                       <  1:2433252/148‑1 (MQ=255)
                                                                                          gCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTaa                                                      >  1:2340141/1‑148 (MQ=255)
                                                                                           cccGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCGTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAAt                                                     <  2:1586143/148‑1 (MQ=255)
                                                                                             cGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGACTCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGa                                                   >  2:3009560/1‑148 (MQ=255)
                                                                                                actactTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGa                                                <  1:2480233/148‑1 (MQ=255)
                                                                                                actactTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGa                                                >  1:2471906/1‑148 (MQ=255)
                                                                                                actactTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGa                                                >  1:166076/1‑148 (MQ=255)
                                                                                                actactTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTGTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGa                                                >  1:2341503/1‑148 (MQ=255)
                                                                                                 ctactTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGAt                                               >  2:2099919/1‑148 (MQ=255)
                                                                                                  tactTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATg                                              >  1:1484898/1‑148 (MQ=255)
                                                                                                  tactTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATg                                              >  2:2538776/1‑148 (MQ=255)
                                                                                                   actTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGa                                             <  2:281473/148‑1 (MQ=255)
                                                                                                   actTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGa                                             <  2:2166426/148‑1 (MQ=255)
                                                                                                   actTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGa                                             <  2:2166607/148‑1 (MQ=255)
                                                                                                      ttttAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGgc                                          >  1:1375795/1‑148 (MQ=255)
                                                                                                      ttttAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGgc                                          >  1:478667/1‑148 (MQ=255)
                                                                                                       tttAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGgcg                                         >  2:220583/1‑148 (MQ=255)
                                                                                                       tttAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGgcg                                         >  1:1028536/1‑148 (MQ=255)
                                                                                                           attaaCATCGCCACAGAACAGGTAGTACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTa                                     >  2:361070/1‑148 (MQ=255)
                                                                                                           attaaCATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTa                                     >  1:3206803/1‑148 (MQ=255)
                                                                                                           attaaCATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTa                                     >  2:3060972/1‑148 (MQ=255)
                                                                                                            ttaaCATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTaa                                    <  2:1347651/148‑1 (MQ=255)
                                                                                                             taaCATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTGCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAAc                                   >  1:3303071/1‑148 (MQ=255)
                                                                                                                cATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACcgc                                >  1:304627/1‑148 (MQ=255)
                                                                                                                cATCGCCACAGAACAGGTAGCAACGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGACGACAGAAGGTAATGAAGATGAGGCTCTTAACcga                                >  2:746164/1‑147 (MQ=255)
                                                                                                                       acaGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCa                         <  2:1152306/148‑1 (MQ=255)
                                                                                                                        caGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCTGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCaa                        <  1:356762/148‑1 (MQ=255)
                                                                                                                         aGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAAt                       <  1:481442/148‑1 (MQ=255)
                                                                                                                         aGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAAt                       <  1:1605123/148‑1 (MQ=255)
                                                                                                                         aGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAAt                       <  1:906384/148‑1 (MQ=255)
                                                                                                                         aGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAAt                       <  1:2273406/148‑1 (MQ=255)
                                                                                                                          gAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATc                      <  1:1651781/148‑1 (MQ=255)
                                                                                                                            aCAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTACCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCtt                    >  2:1981781/1‑148 (MQ=255)
                                                                                                                             cAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTa                   <  2:2734899/148‑1 (MQ=255)
                                                                                                                             cAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTa                   <  2:2734723/148‑1 (MQ=255)
                                                                                                                              aGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTAc                  <  2:1452039/148‑1 (MQ=255)
                                                                                                                               ggTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTAcc                 <  2:2661139/148‑1 (MQ=255)
                                                                                                                                gTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCt                >  1:1464530/1‑148 (MQ=255)
                                                                                                                                gTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCt                <  2:3301068/148‑1 (MQ=255)
                                                                                                                                   gCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGc             >  1:1595593/1‑148 (MQ=255)
                                                                                                                                      ccGCTAAAAGCTTTCCTCGCGGAACATCAGATAGGCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGCaaa          <  2:3496829/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CATTTTCGCTCTCCTTTATGCTGCTGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCGTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGCAAAATACGCGG  >  CP009273/517247‑517536

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCGGCATTTTCGCTCTCCTTTATGCTGCTGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCGTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGCAAAATAC  >  CP009273/517243‑517532
                                                                                                                                                     |                                                                                                                                            
TCGGCATTTTCGCTCTCCTTTATGCTGCTGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCT                                                                                                                                              <  SRR19973066.580291/150‑1 (MQ=60)
    CATTTTCGCTCTCCTTTATGCTGCTGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCC                                                                                                                                          >  SRR19973066.1691356/1‑150 (MQ=60)
     ATTTTCGCTCTCCTTTATGCTGCTGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCT                                                                                                                                         >  SRR19973066.1108918/1‑150 (MQ=60)
     ATTTTCGCTCTCCTTTATGCTGCTGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCT                                                                                                                                         >  SRR19973066.840886/1‑150 (MQ=60)
       TTTCGCTCTCCTTTATGCTGCTGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCG                                                                                                                                       <  SRR19973066.1887809/150‑1 (MQ=60)
            CTCTCCTTTATGCTGCTGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAA                                                                                                                                  >  SRR19973066.2392594/1‑150 (MQ=60)
            CTCTCCTTTATGCTGCTGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAA                                                                                                                                  >  SRR19973066.2392581/1‑150 (MQ=60)
             TCTCCTTTATGCTGCTGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAAC                                                                                                                                 >  SRR19973066.2868220/1‑150 (MQ=60)
              CTCCTTTATGCTGCTGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACA                                                                                                                                >  SRR19973066.116922/1‑150 (MQ=60)
                 CTTTATGCTGCTGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCA                                                                                                                             <  SRR19973066.2928895/150‑1 (MQ=60)
                  TTTATGCTGCTGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCTCTAAAAGCTTTCCTCGCGGAACATCAG                                                                                                                            <  SRR19973066.1468228/150‑1 (MQ=60)
                  TTTATGCTGCTGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAG                                                                                                                            <  SRR19973066.1468272/150‑1 (MQ=60)
                     ATGCTGCTGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATA                                                                                                                         <  SRR19973066.888043/150‑1 (MQ=60)
                      TGCTGCTGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAG                                                                                                                        <  SRR19973066.994687/150‑1 (MQ=60)
                      TGCTGCTGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAG                                                                                                                        <  SRR19973066.2303506/150‑1 (MQ=60)
                        CTGCTGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTC                                                                                                                      <  SRR19973066.1431811/150‑1 (MQ=60)
                            TGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGG                                                                                                                  >  SRR19973066.1932231/1‑150 (MQ=60)
                               CACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAAT                                                                                                               >  SRR19973066.621501/1‑150 (MQ=60)
                                ACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATC                                                                                                              <  SRR19973066.999974/150‑1 (MQ=60)
                                   GCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTT                                                                                                           <  SRR19973066.1761369/150‑1 (MQ=60)
                                   GCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTT                                                                                                           <  SRR19973066.1761384/150‑1 (MQ=60)
                                       CTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTAT                                                                                                       <  SRR19973066.1785560/150‑1 (MQ=60)
                                       CTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTAT                                                                                                       <  SRR19973066.2397311/150‑1 (MQ=60)
                                         GGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCC                                                                                                     <  SRR19973066.1176443/150‑1 (MQ=60)
                                          GTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCG                                                                                                    <  SRR19973066.397378/150‑1 (MQ=60)
                                             TTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTG                                                                                                 <  SRR19973066.2978889/150‑1 (MQ=60)
                                               GCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGT                                                                                               <  SRR19973066.1580944/150‑1 (MQ=60)
                                                  TGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACACAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCG                                                                                            >  SRR19973066.251415/1‑150 (MQ=60)
                                                     CGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGC                                                                                         >  SRR19973066.556092/1‑150 (MQ=60)
                                                      GATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCG                                                                                        >  SRR19973066.823196/1‑150 (MQ=60)
                                                          TGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGC                                                                                    >  SRR19973066.224199/1‑150 (MQ=60)
                                                          TGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGC                                                                                    >  SRR19973066.224235/1‑150 (MQ=60)
                                                             TCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGA                                                                                 <  SRR19973066.132709/150‑1 (MQ=60)
                                                             TCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGA                                                                                 <  SRR19973066.1830151/150‑1 (MQ=60)
                                                                        CAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAAC                                                                      >  SRR19973066.2776361/1‑150 (MQ=60)
                                                                         AGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACG                                                                     >  SRR19973066.2997172/1‑150 (MQ=60)
                                                                         AGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACG                                                                     >  SRR19973066.2992917/1‑150 (MQ=60)
                                                                           CAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGAT                                                                   <  SRR19973066.1413031/150‑1 (MQ=60)
                                                                           CAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGAT                                                                   <  SRR19973066.2249785/150‑1 (MQ=60)
                                                                           CAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGAT                                                                   <  SRR19973066.1413009/150‑1 (MQ=60)
                                                                            AGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATA                                                                  >  SRR19973066.3097435/1‑150 (MQ=60)
                                                                                GCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCC                                                              <  SRR19973066.3546155/150‑1 (MQ=60)
                                                                                     CTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAG                                                         <  SRR19973066.653593/150‑1 (MQ=60)
                                                                                      TCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGA                                                        <  SRR19973066.2775402/150‑1 (MQ=60)
                                                                                       CCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAA                                                       >  SRR19973066.3052866/1‑150 (MQ=60)
                                                                                            AAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAA                                                  >  SRR19973066.2356078/1‑150 (MQ=60)
                                                                                             AGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAAT                                                 <  SRR19973066.2449817/150‑1 (MQ=60)
                                                                                             AGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAAT                                                 <  SRR19973066.2449524/150‑1 (MQ=60)
                                                                                                  GAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGA                                            >  SRR19973066.166569/1‑150 (MQ=60)
                                                                                                  GAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGA                                            >  SRR19973066.544809/1‑150 (MQ=60)
                                                                                                  GAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGA                                            >  SRR19973066.418277/1‑150 (MQ=60)
                                                                                                  GAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGA                                            >  SRR19973066.167577/1‑150 (MQ=60)
                                                                                                  GAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGA                                            >  SRR19973066.2488755/1‑150 (MQ=60)
                                                                                                  GAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTGTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGA                                            >  SRR19973066.2357445/1‑150 (MQ=60)
                                                                                                    ACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATG                                          >  SRR19973066.1495315/1‑150 (MQ=60)
                                                                                                    ACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATG                                          <  SRR19973066.2497142/150‑1 (MQ=60)
                                                                                                    ACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATG                                          <  SRR19973066.2497155/150‑1 (MQ=60)
                                                                                                     CTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGA                                         >  SRR19973066.1160515/1‑150 (MQ=60)
                                                                                                        CTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGC                                      >  SRR19973066.482278/1‑150 (MQ=60)
                                                                                                        CTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGC                                      >  SRR19973066.1385747/1‑150 (MQ=60)
                                                                                                         TTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCG                                     >  SRR19973066.1036108/1‑150 (MQ=60)
                                                                                                         TTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCGTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCG                                     >  SRR19973066.2730024/1‑150 (MQ=60)
                                                                                                             TAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTA                                 >  SRR19973066.3228075/1‑150 (MQ=60)
                                                                                                               ATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTGCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAAC                               >  SRR19973066.3324921/1‑150 (MQ=60)
                                                                                                                  AACATCGCCACAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGC                            >  SRR19973066.306802/1‑150 (MQ=60)
                                                                                                                            CAGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCTGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATC                  <  SRR19973066.359543/150‑1 (MQ=60)
                                                                                                                             AGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCT                 <  SRR19973066.485063/150‑1 (MQ=60)
                                                                                                                             AGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCT                 <  SRR19973066.1616772/150‑1 (MQ=60)
                                                                                                                             AGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCT                 <  SRR19973066.2289031/150‑1 (MQ=60)
                                                                                                                             AGAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCT                 <  SRR19973066.912785/150‑1 (MQ=60)
                                                                                                                              GAACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTT                <  SRR19973066.1663807/150‑1 (MQ=60)
                                                                                                                               AACAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTA               <  SRR19973066.2819675/150‑1 (MQ=60)
                                                                                                                                 CAGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACC             <  SRR19973066.1679979/150‑1 (MQ=60)
                                                                                                                                  AGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCT            >  SRR19973066.3206945/1‑150 (MQ=60)
                                                                                                                                  AGGTAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCT            >  SRR19973066.1474873/1‑150 (MQ=60)
                                                                                                                                     TAGCACCGCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGC         >  SRR19973066.1607143/1‑150 (MQ=60)
                                                                                                                                            GCTAAAAGCTTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGCAAAATAC  <  SRR19973066.875106/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                            
TCGGCATTTTCGCTCTCCTTTATGCTGCTGGCACTGCTGCTGGTGTTGCGTGGCGATCTGCTCGACCGCTGGCAGCAGCAGCTACCTCCAGAAAGCCCGAACTACTTTTTAATTAACATCGCCACAGAACAGGTAGCACCGCTAAAAGCGTTCCTCGCGGAACATCAGATAGTCCCGGAATCGTTTTATCCGGTGGTGCGGGCGCGGCTGACGGCGATTAACGATAAGCCGACAGAAGGTAATGAAGATGAGGCGCTTAACCGCGAACTCAATCTTACCTGGCAAAATAC  >  CP009273/517243‑517532

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑