Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 555,927 G→T 9.1% D259Y (GAT→TAT)  sfmD → putative outer membrane export usher protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273555,9270GT 9.1% 697.7 / 28.7 199D259Y (GAT→TAT) sfmDputative outer membrane export usher protein
Reads supporting (aligned to +/- strand):  ref base G (83/97);  new base T (10/9);  total (93/106)
Fisher's exact test for biased strand distribution p-value = 6.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 204 total aligned reads displayed.

TGGCGTTTACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTAACGATTCGGCAAAATGGTTTTATTA  >  CP009273/555780‑556074
                                                                                                                                                   |                                                                                                                                                   
  gCGTTTACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGat                                                                                                                                                   >  1:1561380/1‑148 (MQ=255)
    gTTTACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGatat                                                                                                                                                 <  2:1555382/148‑1 (MQ=255)
    gTTTACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGatat                                                                                                                                                 <  2:426474/148‑1 (MQ=255)
     tttACGCAACAATGGTTCCTGGTACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATAtt                                                                                                                                                <  1:3047131/148‑1 (MQ=255)
       tACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATAtttt                                                                                                                                              <  1:1996338/148‑1 (MQ=255)
          gCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTTATATTTTCGa                                                                                                                                           <  1:1728591/148‑1 (MQ=255)
          gCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTTATATTTTCGa                                                                                                                                           <  1:2502766/148‑1 (MQ=255)
              caaTGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGc                                                                                                                                       >  2:322136/1‑148 (MQ=255)
               aaTGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCg                                                                                                                                      >  2:2829380/1‑148 (MQ=255)
               aaTGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCg                                                                                                                                      <  1:2331924/148‑1 (MQ=255)
                aTGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGt                                                                                                                                     >  2:125689/1‑148 (MQ=255)
                  ggTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTg                                                                                                                                   >  2:1644519/1‑148 (MQ=255)
                  ggTTACTGGAAATATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGACAATACAGGAAGTGATATTTTCGATGGCGTTg                                                                                                                                   <  1:3304369/148‑1 (MQ=255)
                   gTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTgg                                                                                                                                  <  2:2746494/148‑1 (MQ=255)
                   gTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTgg                                                                                                                                  <  2:2162146/148‑1 (MQ=255)
                    ttCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTTATATTGTCGATGGAGTTGGa                                                                                                                                 >  2:25593/1‑148 (MQ=255)
                        tGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTc                                                                                                                             <  2:1603955/148‑1 (MQ=255)
                        tGGAACTATCTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTc                                                                                                                             <  1:409112/148‑1 (MQ=255)
                          gAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCgt                                                                                                                           >  1:1382386/1‑148 (MQ=255)
                          gAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCgt                                                                                                                           >  2:2064891/1‑148 (MQ=255)
                            aCTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCgtgg                                                                                                                         >  2:2792253/1‑148 (MQ=255)
                            aCTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAAGACAGGAAGTGATATTTTCGATGGCGTTGGATTTCgtgg                                                                                                                         <  1:1468643/148‑1 (MQ=255)
                               aTTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGACGTGATATTTTCGATGGCGTTGGATTTCGTGgtgt                                                                                                                      >  2:760068/1‑148 (MQ=255)
                               aTTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGgtgt                                                                                                                      >  2:884317/1‑148 (MQ=255)
                                   tCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACgg                                                                                                                  <  2:2990272/148‑1 (MQ=255)
                                    cgcgGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGc                                                                                                                 <  2:2479110/148‑1 (MQ=255)
                                     gcgGAAATGGATATCATTCAGAACAGTTGAATATTATTGTCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGAAGGCAATACAGGAAGTTATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCt                                                                                                                <  2:1831103/148‑1 (MQ=255)
                                     gcgGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCt                                                                                                                <  1:3121240/148‑1 (MQ=255)
                                       ggAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCttt                                                                                                              <  1:3445960/148‑1 (MQ=255)
                                        gAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTa                                                                                                             <  2:2023953/148‑1 (MQ=255)
                                         aaaTGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTAt                                                                                                            >  2:1336264/1‑148 (MQ=255)
                                          aaTGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTAtt                                                                                                           >  2:3082412/1‑148 (MQ=255)
                                            tGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTAttct                                                                                                         >  1:1323846/1‑148 (MQ=255)
                                               atatCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATtcttct                                                                                                      >  2:3328694/1‑148 (MQ=255)
                                                  tCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGat                                                                                                   >  1:1061661/1‑148 (MQ=255)
                                                     ttCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGataat                                                                                                >  1:2083920/1‑148 (MQ=255)
                                                       cAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAatat                                                                                              <  1:1700398/148‑1 (MQ=255)
                                                       cAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAatat                                                                                              <  1:1899036/148‑1 (MQ=255)
                                                         gAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGt                                                                                            <  1:2214888/148‑1 (MQ=255)
                                                          aaCAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTa                                                                                           >  2:1854059/1‑148 (MQ=255)
                                                          aaCAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGCTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGTCGTTGGATTTCGTGGTGTAAGGCTTTATTATTCTGATAATATGTa                                                                                           >  1:183534/1‑148 (MQ=255)
                                                            cAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATc                                                                                         <  1:2961834/148‑1 (MQ=255)
                                                            cAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATc                                                                                         >  1:2636081/1‑148 (MQ=255)
                                                            cAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATc                                                                                         <  1:848379/148‑1 (MQ=255)
                                                             aGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATcc                                                                                        <  1:125689/148‑1 (MQ=255)
                                                             aGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATcc                                                                                        <  1:270817/148‑1 (MQ=255)
                                                                ggAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGa                                                                                     >  1:1834997/1‑148 (MQ=255)
                                                                    taataTTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTTATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGc                                                                                 <  1:1616489/148‑1 (MQ=255)
                                                                    taataTTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGc                                                                                 <  1:1000825/148‑1 (MQ=255)
                                                                    taataTTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGc                                                                                 >  1:232438/1‑148 (MQ=255)
                                                                    taataTTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGc                                                                                 <  1:3222851/148‑1 (MQ=255)
                                                                     aataTTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTTATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGcc                                                                                >  2:2212376/1‑148 (MQ=255)
                                                                      atatTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCa                                                                               <  1:393905/148‑1 (MQ=255)
                                                                      atatTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCa                                                                               <  1:2183279/148‑1 (MQ=255)
                                                                       tatTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAg                                                                              >  1:1814963/1‑148 (MQ=255)
                                                                       tatTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAg                                                                              <  2:3053843/148‑1 (MQ=255)
                                                                       tatTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAg                                                                              >  2:305586/1‑148 (MQ=255)
                                                                         ttGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGACGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCa                                                                            >  2:3285921/1‑148 (MQ=255)
                                                                         ttGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCa                                                                            <  1:308317/148‑1 (MQ=255)
                                                                           ggCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAg                                                                          <  1:1625962/148‑1 (MQ=255)
                                                                             cACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAggg                                                                        <  1:1933477/148‑1 (MQ=255)
                                                                             cACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAggg                                                                        <  2:2143409/148‑1 (MQ=255)
                                                                               ccTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTTATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGtt                                                                      >  1:1418143/1‑148 (MQ=255)
                                                                                cTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGttt                                                                     >  1:965451/1‑148 (MQ=255)
                                                                                   ggTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGcc                                                                  >  1:2880113/1‑148 (MQ=255)
                                                                                     tACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGcccc                                                                >  1:3128940/1‑148 (MQ=255)
                                                                                      aCAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCa                                                               <  2:1743848/148‑1 (MQ=255)
                                                                                       cAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCaa                                                              >  2:1036075/1‑148 (MQ=255)
                                                                                        aGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAAc                                                             >  2:3399749/1‑148 (MQ=255)
                                                                                         gcgcgcCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACg                                                            >  1:2044401/1‑148 (MQ=255)
                                                                                         gcgcgcCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACg                                                            <  1:699569/148‑1 (MQ=255)
                                                                                          cgcgcCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACgg                                                           <  2:1503111/148‑1 (MQ=255)
                                                                                           gcgcCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGt                                                          <  1:2792292/148‑1 (MQ=255)
                                                                                              ccATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACg                                                       >  2:2693639/1‑148 (MQ=255)
                                                                                               cATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGt                                                      >  2:3437221/1‑148 (MQ=255)
                                                                                                    ttCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTTATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGAt                                                 >  1:1144912/1‑148 (MQ=255)
                                                                                                    ttCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTTATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGAt                                                 >  2:2341537/1‑148 (MQ=255)
                                                                                                       cGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGc                                              <  2:1518246/148‑1 (MQ=255)
                                                                                                              aaGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTAcg                                       >  2:709137/1‑148 (MQ=255)
                                                                                                               aGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTTATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTAcgg                                      >  2:1613413/1‑148 (MQ=255)
                                                                                                                 tGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTAcggcg                                    <  1:3504524/148‑1 (MQ=255)
                                                                                                                     cTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGccc                                >  2:844905/1‑148 (MQ=255)
                                                                                                                     cTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGccc                                <  2:1371163/148‑1 (MQ=255)
                                                                                                                          aaTGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTa                           <  2:2368414/148‑1 (MQ=255)
                                                                                                                          aaTGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTa                           <  2:630246/148‑1 (MQ=255)
                                                                                                                          aaTGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTa                           <  1:2829380/148‑1 (MQ=255)
                                                                                                                           aTGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTaa                          <  2:573433/148‑1 (MQ=255)
                                                                                                                             gggAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTAACg                        <  2:2042054/148‑1 (MQ=255)
                                                                                                                                 gaCGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTAACGATTc                    <  1:887416/148‑1 (MQ=255)
                                                                                                                                 gaCGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTAACGATTc                    >  1:3393093/1‑148 (MQ=255)
                                                                                                                                  aCGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTAACGATTCg                   <  2:1481301/148‑1 (MQ=255)
                                                                                                                                  aCGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTAACGATTCg                   <  2:1078968/148‑1 (MQ=255)
                                                                                                                                        aTACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTAACGATTCGGCaaaa             >  1:2242897/1‑148 (MQ=255)
                                                                                                                                          aCAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATCTGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTCACGATTCGGCACAATg           >  2:3372493/1‑148 (MQ=255)
                                                                                                                                            aGGAAGTTATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTAACGATTCGGCAAAATGGt         >  1:59554/1‑148 (MQ=255)
                                                                                                                                            aGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTAACGATTCGGCAAAATGGt         >  1:1672251/1‑148 (MQ=255)
                                                                                                                                             ggAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTAACGATTCGGCAAAATGGtt        >  1:2784866/1‑148 (MQ=255)
                                                                                                                                                  tGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTAACGATTCGGCAAAATGGTTttatt   <  2:1982259/148‑1 (MQ=255)
                                                                                                                                                  tGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTAACGATTCGGCAAAATGGTTttatt   <  1:2064891/148‑1 (MQ=255)
                                                                                                                                                   gATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTAACGATTCGGCAAAATGGTTttatta  <  1:3364549/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TGGCGTTTACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTAACGATTCGGCAAAATGGTTTTATTA  >  CP009273/555780‑556074

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A