Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 555,927 G→T 26.3% D259Y (GAT→TAT)  sfmD → putative outer membrane export usher protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273555,9270GT26.3% 789.0 / 223.8 357D259Y (GAT→TAT) sfmDputative outer membrane export usher protein
Reads supporting (aligned to +/- strand):  ref base G (128/135);  new base T (44/50);  total (172/185)
Fisher's exact test for biased strand distribution p-value = 8.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 359 total aligned reads displayed.

CGTGGCGTTTACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTAACGATTCGGCAAAATGGTTTTATTATC  >  CP009273/555778‑556076
                                                                                                                                                     |                                                                                                                                                     
  tGGCGTTTACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTTat                                                                                                                                                     <  2:3644426/150‑1 (MQ=255)
   ggCGTTTACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGata                                                                                                                                                    <  2:4518234/150‑1 (MQ=255)
      gTTTACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGTAATATTATTGGCAACTGGGTACAGCGCGCCATTATTCAGCTGAAAAGTGAACTGGTATTGGGAGACGGCAATACAGGAAGTGATAttt                                                                                                                                                 <  2:3684363/150‑1 (MQ=255)
      gTTTACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATAttt                                                                                                                                                 <  1:818331/150‑1 (MQ=255)
        ttACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTTATATTTTc                                                                                                                                               >  2:2694442/1‑150 (MQ=255)
              aacaaTGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTTATATTTTCGATGGc                                                                                                                                         <  2:4458285/150‑1 (MQ=255)
               acaaTGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTTATATTTTCGATGGCg                                                                                                                                        <  1:3247276/150‑1 (MQ=255)
                 aaTGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGACGTGATATTTTCGATGGCGtt                                                                                                                                      >  1:4066787/1‑150 (MQ=255)
                  aTGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTg                                                                                                                                     <  2:1769424/150‑1 (MQ=255)
                   tGGTTCCTGGAACTATGTTAGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTgg                                                                                                                                    <  1:1188058/150‑1 (MQ=255)
                     ggtCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGAt                                                                                                                                  <  1:3764407/148‑1 (MQ=255)
                     gTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATATTCGATGGCGTTGGAt                                                                                                                                  >  2:592375/1‑150 (MQ=255)
                      ttCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGAtt                                                                                                                                 <  1:1467925/150‑1 (MQ=255)
                           ggAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTTATATTTTCGATGGCGTTGGATTTCgtg                                                                                                                            >  2:5293596/1‑150 (MQ=255)
                           ggAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTTATATTTTCGATGGCGTTGGATTTCgtg                                                                                                                            >  2:1430190/1‑150 (MQ=255)
                           ggAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTTATATTTTCGATGGCGTTGGATTTCgtg                                                                                                                            >  2:3104566/1‑150 (MQ=255)
                           ggAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCgtg                                                                                                                            <  1:1061151/150‑1 (MQ=255)
                            gAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCgtgg                                                                                                                           >  2:3204615/1‑150 (MQ=255)
                             aaCTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTTATATTTTCGATGGCGTTGGATTTCgtggt                                                                                                                          >  2:4293228/1‑150 (MQ=255)
                             aaCTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTTATATTTTCGATGGCGTTGGATTTCgtggt                                                                                                                          >  1:1961209/1‑150 (MQ=255)
                              aCTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTTATATTTTCGATGGCGTTGGATTTCgtggtg                                                                                                                         >  1:1666199/1‑150 (MQ=255)
                               cTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGgtgt                                                                                                                        >  1:976059/1‑150 (MQ=255)
                               cTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGgtgt                                                                                                                        >  1:1408858/1‑150 (MQ=255)
                                gaTTGTCGCGGAAATGGATATCATTCAGAACAGTGGAATACTATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGTCGGCAATACAGGAAGTGATCTTTTCGATGGCGTTGGATTTCGTGGTGTa                                                                                                                       <  2:2520055/149‑1 (MQ=255)
                                 aTTTTCGCTGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTAc                                                                                                                      <  2:740993/150‑1 (MQ=255)
                                 aTTTTCGCGGAACTGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATAATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTAc                                                                                                                      >  1:443492/1‑150 (MQ=255)
                                 aTTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTAc                                                                                                                      >  1:3716339/1‑150 (MQ=255)
                                   tttCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACgg                                                                                                                    >  2:1561756/1‑150 (MQ=255)
                                      cgcgGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTTATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCtt                                                                                                                 <  1:2863561/150‑1 (MQ=255)
                                       gcgGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTTATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCttt                                                                                                                <  1:296898/150‑1 (MQ=255)
                                        cgGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTa                                                                                                               <  2:1858649/150‑1 (MQ=255)
                                         ggAAATGGATATCACTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTAt                                                                                                              <  2:2934268/150‑1 (MQ=255)
                                            aaTGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTAttct                                                                                                           <  2:3751199/150‑1 (MQ=255)
                                               ggATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATtcttct                                                                                                        <  1:2496268/150‑1 (MQ=255)
                                                gATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGGTGGATTTCGTGGTGTACGGCTTTATTCTTCTg                                                                                                       >  2:396319/1‑150 (MQ=255)
                                                 atatCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGa                                                                                                      >  1:2342902/1‑150 (MQ=255)
                                                 atatCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGa                                                                                                      >  2:1271763/1‑150 (MQ=255)
                                                  tataATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGat                                                                                                     <  1:2544853/150‑1 (MQ=255)
                                                  tatCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGat                                                                                                     >  1:5312641/1‑150 (MQ=255)
                                                    tCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGataa                                                                                                   >  2:1289075/1‑150 (MQ=255)
                                                     cATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGataat                                                                                                  >  2:3900791/1‑150 (MQ=255)
                                                     cATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGataat                                                                                                  <  2:2577331/150‑1 (MQ=255)
                                                       ttCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTTATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAatat                                                                                                >  1:3840929/1‑150 (MQ=255)
                                                        tCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATg                                                                                               <  2:2276727/150‑1 (MQ=255)
                                                         cAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGt                                                                                              >  2:3529177/1‑150 (MQ=255)
                                                         cAGAACAGTGGAATAATATTGGCACCTGGGTAACGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTTATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGt                                                                                              >  1:355938/1‑150 (MQ=255)
                                                           gAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTAt                                                                                            <  2:5135947/150‑1 (MQ=255)
                                                             aCAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATcc                                                                                          >  1:4981063/1‑150 (MQ=255)
                                                              cAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTTATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCt                                                                                         <  2:1142020/150‑1 (MQ=255)
                                                              cAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTTATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCt                                                                                         <  2:5105001/150‑1 (MQ=255)
                                                              cAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCt                                                                                         <  1:3194517/150‑1 (MQ=255)
                                                              cAGTGGAATAATATTGGCACCGGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTTATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCt                                                                                         <  2:4798849/150‑1 (MQ=255)
                                                               aGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTg                                                                                        >  1:655190/1‑150 (MQ=255)
                                                               aGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTg                                                                                        >  1:1770756/1‑150 (MQ=255)
                                                                 tGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGGTGGATTTCGGGGTGTACGGCTTTCTTCTTCTGATAATATGTATCCTGAt                                                                                      >  2:5046950/1‑150 (MQ=255)
                                                                  ggAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTTATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATa                                                                                     <  1:592025/150‑1 (MQ=255)
                                                                   gAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAg                                                                                    >  2:2057437/1‑150 (MQ=255)
                                                                   gAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAg                                                                                    >  2:1610893/1‑150 (MQ=255)
                                                                    aataatATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGc                                                                                   >  2:4717652/1‑150 (MQ=255)
                                                                    aataatATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGc                                                                                   >  2:4382624/1‑150 (MQ=255)
                                                                      taataTTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTTATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCa                                                                                 >  2:4198957/1‑150 (MQ=255)
                                                                          atTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCaa                                                                             >  1:3400575/1‑150 (MQ=255)
                                                                           ttGGCACCTGGGTACAGCGCGCCATTATTCCTCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTAGAGGTGTACGGCTTTATTCTTCTGATAAAATGTATCATGATAGCCAGAAAg                                                                            >  1:3144117/1‑150 (MQ=255)
                                                                             ggCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAggg                                                                          <  2:189793/150‑1 (MQ=255)
                                                                                  cTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTTATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGc                                                                     <  1:3305204/150‑1 (MQ=255)
                                                                                      gTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCa                                                                 >  2:3399614/1‑150 (MQ=255)
                                                                                        aCAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAAc                                                               <  1:2954220/150‑1 (MQ=255)
                                                                                              cgcCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACg                                                         >  1:2409472/1‑150 (MQ=255)
                                                                                               gcCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGt                                                        >  1:5189499/1‑150 (MQ=255)
                                                                                                ccATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTg                                                       >  1:2226101/1‑150 (MQ=255)
                                                                                                ccATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTg                                                       <  2:457328/150‑1 (MQ=255)
                                                                                                     attCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGAtt                                                  >  2:197150/1‑150 (MQ=255)
                                                                                                      ttCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTTATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTg                                                 >  1:4187134/1‑150 (MQ=255)
                                                                                                          gCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTTATATTTTCGATGGCGTGGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCg                                             >  2:888527/1‑150 (MQ=255)
                                                                                                          gCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCg                                             <  1:2666738/150‑1 (MQ=255)
                                                                                                           cTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGt                                            >  1:4743353/1‑150 (MQ=255)
                                                                                                                aaGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATAGCCCGTAcggc                                       >  2:2745816/1‑150 (MQ=255)
                                                                                                                      aCTGGTAATGGGAGACGGCAATACAGGAAGTTATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCa                                 <  2:3582208/150‑1 (MQ=255)
                                                                                                                         ggTAATGGGAGACGGCAATACAGGAAGTTATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCt                              <  1:4473243/150‑1 (MQ=255)
                                                                                                                           tAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTaa                            >  2:5321957/1‑150 (MQ=255)
                                                                                                                           tAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTaa                            >  2:1655104/1‑150 (MQ=255)
                                                                                                                            aaTGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTAAc                           <  1:3139354/150‑1 (MQ=255)
                                                                                                                             aTGGGAGACGGCAATACAGGAAGTTATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTAACg                          <  2:4619426/150‑1 (MQ=255)
                                                                                                                                  agaCGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTAACGATTCg                     <  2:4914719/150‑1 (MQ=255)
                                                                                                                                  agaCGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTAACGATTCg                     >  1:2683786/1‑150 (MQ=255)
                                                                                                                                       gCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTAACGATTCGGCaaa                <  2:2791914/150‑1 (MQ=255)
                                                                                                                                        cAATACAGGAAGTTATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTAACGATTCGGCaaaa               >  1:233023/1‑150 (MQ=255)
                                                                                                                                         aaTACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTAACGATTCGGCAAAAt              <  1:3904740/150‑1 (MQ=255)
                                                                                                                                          aTACAGGAAGTTATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTAACGATTCGGCAAAATg             >  2:2392104/1‑150 (MQ=255)
                                                                                                                                          aTACAGGAAGTTATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCACCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTAACGATTCGGCAAAATg             >  1:1584257/1‑150 (MQ=255)
                                                                                                                                           tACAGGAAGTTATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTAACGATTCGGCAAAATgg            <  2:4042975/150‑1 (MQ=255)
                                                                                                                                           tACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTAACGATTCGGCAAAATgg            <  1:4887862/150‑1 (MQ=255)
                                                                                                                                              aGGAAGTTATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTAACGATTCg                     <  2:2331627/138‑1 (MQ=255)
                                                                                                                                              aGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTAACGATTCGGCAAAATGGttt         >  2:2664022/1‑150 (MQ=255)
                                                                                                                                               ggAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTAACGATTCGGCAAAATGGtttt        <  2:783201/150‑1 (MQ=255)
                                                                                                                                                gAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTAACGATTCGGCAAAATGGTTtta       >  2:3148620/1‑150 (MQ=255)
                                                                                                                                                 aaGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTAACGATTCGGCAAAATGGTTttat      <  2:3018442/150‑1 (MQ=255)
                                                                                                                                                   gTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTAACGATTCGGCAAAATGGTTttatta    <  1:4563056/150‑1 (MQ=255)
                                                                                                                                                    tGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTAACGATTCGGCAAAATGGTTTtattat   <  1:367008/150‑1 (MQ=255)
                                                                                                                                                     gATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTAACGATTCGGCAAAATGGTTTTATtatc  >  2:1382346/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGTGGCGTTTACGCAACAATGGTTCCTGGAACTATTTTCGCGGAAATGGATATCATTCAGAACAGTGGAATAATATTGGCACCTGGGTACAGCGCGCCATTATTCCGCTGAAAAGTGAACTGGTAATGGGAGACGGCAATACAGGAAGTGATATTTTCGATGGCGTTGGATTTCGTGGTGTACGGCTTTATTCTTCTGATAATATGTATCCTGATAGCCAGCAAGGGTTTGCCCCAACGGTACGTGGGATTGCCCGTACGGCGGCCCAGCTAACGATTCGGCAAAATGGTTTTATTATC  >  CP009273/555778‑556076

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A