Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,796,101 C→A 100% D243Y (GAC→TAC)  thrS ← threonyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,796,1010CA95.4% 588.4 / 15.4 208D243Y (GAC→TAC) thrSthreonyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base C (6/4);  new base A (104/94);  total (110/98)
Fisher's exact test for biased strand distribution p-value = 7.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 212 total aligned reads displayed.

TGATACTGGTACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTCGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTTCATTAGTTTGAAATGA  >  CP009273/1795956‑1796246
                                                                                                                                                 |                                                                                                                                                 
tGATACTGGTACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTagc                                                                                                                                                 >  2:3327158/1‑148 (MQ=255)
tGATACTGGTACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTagc                                                                                                                                                 >  2:1892619/1‑148 (MQ=255)
 gATACTGGTACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTagcg                                                                                                                                                >  1:1051799/1‑148 (MQ=255)
  aTACTGGTACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTCGCGt                                                                                                                                               >  2:731914/1‑148 (MQ=255)
  aTACTGGTACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGt                                                                                                                                               <  1:1159721/148‑1 (MQ=255)
  aTACTGGTACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGGAGCGt                                                                                                                                               >  1:1179499/1‑148 (MQ=255)
      tGGTACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTcg                                                                                                                                           >  2:353556/1‑148 (MQ=255)
        gTACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTcgcg                                                                                                                                         <  2:1134614/148‑1 (MQ=255)
          aCTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTCGCGTTTCGCGGc                                                                                                                                       <  2:640694/148‑1 (MQ=255)
                tCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCttcttc                                                                                                                                 >  2:2651338/1‑148 (MQ=255)
                 cAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTcc                                                                                                                                <  2:1745557/148‑1 (MQ=255)
                     ttAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGgc                                                                                                                            >  1:1342057/1‑148 (MQ=255)
                     ttAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGgc                                                                                                                            >  2:1254490/1‑148 (MQ=255)
                      tAGAACGAACAAACACGTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGgcg                                                                                                                           <  1:3009383/148‑1 (MQ=255)
                       aGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGgcgc                                                                                                                          <  2:640843/148‑1 (MQ=255)
                         aacgaacAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTg                                                                                                                        >  1:1079841/1‑148 (MQ=255)
                         aacgaacAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTg                                                                                                                        >  2:3168942/1‑148 (MQ=255)
                         aacgaacAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTAAAGGTCGAGCGGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTg                                                                                                                        >  1:2159276/1‑148 (MQ=255)
                          acgaacAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGc                                                                                                                       >  1:848676/1‑148 (MQ=255)
                          acgaacAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGc                                                                                                                       >  1:848659/1‑148 (MQ=255)
                           cgaacAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCa                                                                                                                      >  1:2455537/1‑148 (MQ=255)
                              acaaacaCTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGt                                                                                                                   <  1:1936907/148‑1 (MQ=255)
                               caaacaCTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTa                                                                                                                  <  2:2931502/148‑1 (MQ=255)
                               caaacaCTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTa                                                                                                                  <  1:2643516/148‑1 (MQ=255)
                                  acacTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGc                                                                                                               >  1:415179/1‑148 (MQ=255)
                                     cTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTAAGGTGGTAGCGTTTCGCGGCTTCTTCAAGGCGCTGCAGGTAAGCGtt                                                                                                            >  1:2371640/1‑148 (MQ=255)
                                      ttCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTAACGAGTTTACGGGGGTAGCGTTTAGCGGCGTCTTCCAGGCGCTGCCGGTAAGCGTTa                                                                                                           >  1:690967/1‑148 (MQ=255)
                                        ccAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAg                                                                                                         >  2:3261206/1‑148 (MQ=255)
                                        ccAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAg                                                                                                         <  2:1775348/148‑1 (MQ=255)
                                        ccAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAg                                                                                                         >  1:3215740/1‑148 (MQ=255)
                                        ccAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAg                                                                                                         >  1:3214808/1‑148 (MQ=255)
                                          aGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTg                                                                                                       <  2:1365675/148‑1 (MQ=255)
                                            ttCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCGGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGGTAAGTGCt                                                                                                     >  2:1614317/1‑148 (MQ=255)
                                             tCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATTGTACAGGTCGAGCTGTTTACCGATTTTAAGGTGGTAGCGTTTCGCGGCTTATTACAGGCGCTGCAGGTAAGCGGTTAGTGCtt                                                                                                    >  1:3438093/1‑148 (MQ=255)
                                               aCGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCtttt                                                                                                  >  2:3137610/1‑148 (MQ=255)
                                               aCGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCGTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCtttt                                                                                                  <  1:1411990/148‑1 (MQ=255)
                                                   aaGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGt                                                                                              <  1:305338/148‑1 (MQ=255)
                                                   aaGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGt                                                                                              <  2:2986532/148‑1 (MQ=255)
                                                    aGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCAAATGGTACAGGTCGAGCTGTTTAACGCATTTAAGGTGGTAGCGTTTAGCGGCGTCTTCCAGGCGCTGCAGGTAAGCGGTTAGTGCTTTTTTGTc                                                                                             >  1:2411345/1‑148 (MQ=255)
                                                      aTGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTg                                                                                           >  2:297700/1‑148 (MQ=255)
                                                              gCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTTCGGGGGTAGCGTTTAGCGGCGTGTTACAGGCGCGGCAGGTAAGCGGTAAGTGCTTTTTTTTCTGCCCACGcc                                                                                   >  1:97181/1‑148 (MQ=255)
                                                              gCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGcc                                                                                   >  2:2815099/1‑148 (MQ=255)
                                                              gCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGcc                                                                                   >  2:3073009/1‑148 (MQ=255)
                                                               ccGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGccg                                                                                  >  2:1958177/1‑148 (MQ=255)
                                                               ccGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGccg                                                                                  <  2:3285911/148‑1 (MQ=255)
                                                               ccGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGccg                                                                                  <  1:1598279/148‑1 (MQ=255)
                                                               ccGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGccg                                                                                  >  2:1242033/1‑148 (MQ=255)
                                                               ccGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTACTGCATAAGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGGTTAGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTTTCTGCCCccgccg                                                                                  >  1:773125/1‑148 (MQ=255)
                                                               ccGTCGTTGTGCCAGAATACCATACCCGGCGCGTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTCGCGGTTCGCGGCGTCTTCCAGGCGCTGCAGGGAAGCGTTAAGTGCTTTTTTTTCTGCCCACCccg                                                                                  >  1:3077631/1‑148 (MQ=255)
                                                                  tcgtTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGccgtac                                                                               >  2:1041448/1‑148 (MQ=255)
                                                                  tcgtTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGccgtac                                                                               >  2:1923240/1‑148 (MQ=255)
                                                                  tcgtTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGccgtac                                                                               >  2:125051/1‑148 (MQ=255)
                                                                      tgtgCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCGGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTACAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGccgtaccgta                                                                           >  2:1617162/1‑148 (MQ=255)
                                                                       gtgCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTaa                                                                          <  2:1203790/148‑1 (MQ=255)
                                                                       gtgCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTaa                                                                          <  2:3097999/148‑1 (MQ=255)
                                                                       gtgCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTaa                                                                          <  2:2975475/148‑1 (MQ=255)
                                                                        tgCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTaaa                                                                         >  2:832656/1‑148 (MQ=255)
                                                                        tgCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTaaa                                                                         <  1:3327412/148‑1 (MQ=255)
                                                                         gCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAAt                                                                        <  2:1678732/148‑1 (MQ=255)
                                                                             gAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGt                                                                    <  2:1793314/148‑1 (MQ=255)
                                                                               ataccataccCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTg                                                                  <  1:3549394/148‑1 (MQ=255)
                                                                                  ccataccCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTAGCGGCTTATTCCAGGCGCAGCAGGTAATCGTTAAGTGCTTTTTTTTCTGCCCACGCCGTACCGTAAATAAGTTGCaa                                                               >  1:532562/1‑148 (MQ=255)
                                                                                    ataccCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTAACGATTTTACGGTGGTAGCGTTTAGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTAACGTAAATAAGTTGCAACa                                                             >  2:300228/1‑148 (MQ=255)
                                                                                     taccCGGCGCTTCTTCCTGCAAATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACAt                                                            >  2:1290012/1‑148 (MQ=255)
                                                                                      accCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACAtt                                                           <  2:1079841/148‑1 (MQ=255)
                                                                                         cGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTttg                                                        <  2:1117059/148‑1 (MQ=255)
                                                                                              cttcttCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTttgttgtt                                                   >  1:951923/1‑148 (MQ=255)
                                                                                              cttcttCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTttgttgtt                                                   >  1:1284202/1‑148 (MQ=255)
                                                                                              cttcttCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTttgttgtt                                                   >  2:590754/1‑148 (MQ=255)
                                                                                                tcttcCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGc                                                 >  2:2281970/1‑148 (MQ=255)
                                                                                                 cttcCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCt                                                >  1:1655619/1‑148 (MQ=255)
                                                                                                 cttcCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCt                                                >  1:1946993/1‑148 (MQ=255)
                                                                                                  ttcCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTg                                               <  1:2543140/148‑1 (MQ=255)
                                                                                                   tcCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGt                                              <  1:2979302/148‑1 (MQ=255)
                                                                                                     cTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTcg                                            <  1:2530878/148‑1 (MQ=255)
                                                                                                      tGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTcgc                                           >  2:3431567/1‑148 (MQ=255)
                                                                                                           atGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTcgccacgc                                      >  2:2577496/1‑148 (MQ=255)
                                                                                                           atGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTcgccacgc                                      >  2:382295/1‑148 (MQ=255)
                                                                                                           atGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTcgccacgc                                      <  1:3116193/148‑1 (MQ=255)
                                                                                                             ggTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTcgccacgcca                                    <  1:2030712/148‑1 (MQ=255)
                                                                                                             ggTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTcgccacgcca                                    >  1:3129940/1‑148 (MQ=255)
                                                                                                              gTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAg                                   <  2:2455537/148‑1 (MQ=255)
                                                                                                               tACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGt                                  <  2:2720046/148‑1 (MQ=255)
                                                                                                               tACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGt                                  >  1:15610/1‑148 (MQ=255)
                                                                                                               tACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGt                                  >  1:2233323/1‑148 (MQ=255)
                                                                                                                    gTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGcc                             >  1:3310464/1‑148 (MQ=255)
                                                                                                                      cGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGcccc                           >  1:1515618/1‑148 (MQ=255)
                                                                                                                        aGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTg                         >  1:2732706/1‑148 (MQ=255)
                                                                                                                         gCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGc                        <  1:353556/148‑1 (MQ=255)
                                                                                                                          cTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGcc                       <  1:1242033/148‑1 (MQ=255)
                                                                                                                            gTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGt                     >  1:2991916/1‑148 (MQ=255)
                                                                                                                             tttACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGtg                    >  1:537263/1‑147 (MQ=255)
                                                                                                                              ttACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGttt                   <  2:566889/148‑1 (MQ=255)
                                                                                                                               tACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGtttt                  <  2:13437/148‑1 (MQ=255)
                                                                                                                                aCCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACTCCAGTAAGCCCCTGCCGTATTc                 >  2:2631542/1‑148 (MQ=255)
                                                                                                                                 ccGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTGTTCa                >  1:2875634/1‑148 (MQ=255)
                                                                                                                                     ttttACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTTCATTAg            <  1:3168262/148‑1 (MQ=255)
                                                                                                                                      tttACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTTCATTAGt           <  1:1851804/148‑1 (MQ=255)
                                                                                                                                           ggtggtAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTTCATTAGTTTGaa      <  2:3469450/148‑1 (MQ=255)
                                                                                                                                              ggtAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTTAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTTCATTAGTTTGAAatg   >  2:2414741/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
TGATACTGGTACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTCGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTTCATTAGTTTGAAATGA  >  CP009273/1795956‑1796246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCTGATACTGGTACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTCGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTTCATTAGTTTGAAATGATG  >  CP009273/1795954‑1796248
                                                                                                                                                   |                                                                                                                                                   
CCTGATACTGGTACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGC                                                                                                                                                   <  SRR19973067.897093/150‑1 (MQ=60)
CCTGATACTGGTACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGC                                                                                                                                                   <  SRR19973067.897774/150‑1 (MQ=60)
 CTGATACTGGTACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCG                                                                                                                                                  >  SRR19973067.1059013/1‑150 (MQ=60)
  TGATACTGGTACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGGAGCGT                                                                                                                                                 >  SRR19973067.1187292/1‑150 (MQ=60)
  TGATACTGGTACTCGTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGT                                                                                                                                                 >  SRR19973067.1186488/1‑150 (MQ=60)
   GATACTGGTACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTCGCGTT                                                                                                                                                >  SRR19973067.1167103/1‑150 (MQ=60)
    ATACTGGTACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATTGTACAGGTCGAGCTGTTTAACGATTTTAACGTGGTAGCGTTT                                                                                                                                               >  SRR19973067.2225402/1‑150 (MQ=60)
    ATACTGGTACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTT                                                                                                                                               <  SRR19973067.1167446/150‑1 (MQ=60)
    ATACTGGTACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTAAGGTGGTAGCGTTT                                                                                                                                               >  SRR19973067.1957622/1‑150 (MQ=60)
      ACTGGTACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCG                                                                                                                                             <  SRR19973067.2435747/150‑1 (MQ=60)
          GTACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTAGCGGC                                                                                                                                         >  SRR19973067.3201134/1‑150 (MQ=60)
                TTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTC                                                                                                                                   >  SRR19973067.1490844/1‑150 (MQ=60)
                  TCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCA                                                                                                                                 <  SRR19973067.1545713/150‑1 (MQ=60)
                     GTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGC                                                                                                                              >  SRR19973067.1351234/1‑150 (MQ=60)
                     GTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGC                                                                                                                              <  SRR19973067.2981557/150‑1 (MQ=60)
                     GTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTAACGAATTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGC                                                                                                                              >  SRR19973067.597374/1‑150 (MQ=60)
                        TAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTGCAGGCGCT                                                                                                                           >  SRR19973067.3147450/1‑150 (MQ=60)
                        TAGAACGAACAAACACGTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCT                                                                                                                           <  SRR19973067.3028523/150‑1 (MQ=60)
                         AGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCCTATGGTACAGGTCGAGCTGTTTAACGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTG                                                                                                                          >  SRR19973067.1086772/1‑150 (MQ=60)
                         AGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATTGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTAGCGGCTTCTTCCAGGCGCTG                                                                                                                          >  SRR19973067.960766/1‑150 (MQ=60)
                         AGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTAACGATTTTACGGGGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTG                                                                                                                          >  SRR19973067.1087235/1‑150 (MQ=60)
                         AGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTAAAGGTCGAGCGGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTG                                                                                                                          >  SRR19973067.2173712/1‑150 (MQ=60)
                          GAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGC                                                                                                                         >  SRR19973067.854402/1‑150 (MQ=60)
                          GAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGC                                                                                                                         >  SRR19973067.854412/1‑150 (MQ=60)
                          GAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGC                                                                                                                         >  SRR19973067.854419/1‑150 (MQ=60)
                           AACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTAACGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCA                                                                                                                        >  SRR19973067.2471680/1‑150 (MQ=60)
                             CGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGG                                                                                                                      <  SRR19973067.390835/150‑1 (MQ=60)
                                ACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAA                                                                                                                   <  SRR19973067.1950116/150‑1 (MQ=60)
                                 CAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAG                                                                                                                  <  SRR19973067.2660649/150‑1 (MQ=60)
                                 CAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAG                                                                                                                  <  SRR19973067.2660589/150‑1 (MQ=60)
                                  AAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGC                                                                                                                 >  SRR19973067.418061/1‑150 (MQ=60)
                                     CACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTAAGGTGGTAGCGTTTCGCGGCTTCTTCAAGGCGCTGCAGGTAAGCGTT                                                                                                              >  SRR19973067.2387065/1‑150 (MQ=60)
                                     CACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTAAGGTGGTAGCGGTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTT                                                                                                              >  SRR19973067.985782/1‑150 (MQ=60)
                                     CACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCGTCCAGCAAATGGTAAAGGTCGCGCTGTTTACCGATTTTAGGGTGGTAGCGGTTAGCGGCTTATTCCAGGagctgacggtaaagggt                                                                                                              >  SRR19973067.2776245/1‑133 (MQ=60)
                                      ACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTAACGAGTTTACGGGGGTAGCGTTTAGCGGCGTCTTCCAGGCGCTGCCGGTAAGCGTTA                                                                                                             >  SRR19973067.695728/1‑150 (MQ=60)
                                        TTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAG                                                                                                           >  SRR19973067.2297853/1‑150 (MQ=60)
                                        TTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAG                                                                                                           >  SRR19973067.3235977/1‑150 (MQ=60)
                                        TTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAG                                                                                                           >  SRR19973067.3235042/1‑150 (MQ=60)
                                        TTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCGTCTTCCAGGCGCTGCAGGTAAGCGTTAAG                                                                                                           >  SRR19973067.3235970/1‑150 (MQ=60)
                                        TTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTAACGCTTTTACGGGGGTAGCGTTTCGCGGCTTCTTACAGGCGCTGCAGGTAAGCGgttag                                                                                                           >  SRR19973067.1636449/1‑145 (MQ=60)
                                            AGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGGATATGGTACAGGTCGAGCTGTTTTACCATTTTAAGGTGGTAGCGTTTGGCGGCTTATTCCAGGCGCTGGAGGTAAGCGTTAAGTGCT                                                                                                       >  SRR19973067.772313/1‑150 (MQ=60)
                                            AGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCAGCATATGGTACAGGTCGAGCTGTTTAACGAATTTACGGTGGTAGCGTTTAGCGGCTTCTTCCAGGCGCTGCAGGTAACCGGTAAGTGCT                                                                                                       >  SRR19973067.2565880/1‑150 (MQ=60)
                                             GTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATTGTACAGGTCGAGCTGTTTACCGATTTTAAGGTGGTAGCGTTTCGCGGCTTATTACAGGCGCTGCAGGTAAGCGGTTAGTGCTT                                                                                                      >  SRR19973067.3459983/1‑150 (MQ=60)
                                                 ACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCGTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTT                                                                                                  <  SRR19973067.1421550/150‑1 (MQ=60)
                                                   GGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTGTTGCTGCATATGGTACAGGTCGAGCTGTTTAACGATTTTTAGGTGGTATCGTTTAGCGGCGTCTTCCAGGCGCTGCAGGTAAGCGgttatttctttttttt                                                                                                >  SRR19973067.321087/1‑134 (MQ=60)
                                                    GAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCAAATGGTACAGGTCGAGCTGTTTAACGCATTTAAGGTGGTAGCGTTTAGCGGCGTCTTCCAGGCGCTGCAGGTAAGCGGTTAGTGCTTTTTTGTC                                                                                               >  SRR19973067.2427117/1‑150 (MQ=60)
                                                     AAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCT                                                                                              <  SRR19973067.307310/150‑1 (MQ=60)
                                                              CAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTTCGGGGGTAGCGTTTAGCGGCGTGTTACAGGCGCGGCAGGTAAGCGGTAAGTGCTTTTTTTTCTGCCCACGCC                                                                                     >  SRR19973067.98185/1‑150 (MQ=60)
                                                              CAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTAGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGGTAAGTGCTTTTTTGTCTGCCCACGCC                                                                                     >  SRR19973067.1784207/1‑150 (MQ=60)
                                                               AGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTACTGCATAAGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGGTTAGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTTTCTGCCCCCGCCG                                                                                    >  SRR19973067.778483/1‑150 (MQ=60)
                                                               AGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCATCCTGCATATGGTACAGGTCGAGCTGTTTAACGATTTTAAGGTGGTCGCGGTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGGTAAGTGCTTTTTTTTCTGCCCACcccc                                                                                    >  SRR19973067.3097227/1‑146 (MQ=60)
                                                               AGCCGTCGTTGTGCCAGAATACCATACCCGGCGCGTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTCGCGGTTCGCGGCGTCTTCCAGGCGCTGCAGGGAAGCGTTAAGTGCTTTTTTTTCTGCCCACCCCG                                                                                    >  SRR19973067.3097217/1‑150 (MQ=60)
                                                                 CCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTA                                                                                  <  SRR19973067.1609252/150‑1 (MQ=60)
                                                                         GTGCCAGAATACCATACCCGGCGCTTCGTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAAT                                                                          <  SRR19973067.1987253/150‑1 (MQ=60)
                                                                          TGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATA                                                                         <  SRR19973067.3348461/150‑1 (MQ=60)
                                                                          TGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATA                                                                         <  SRR19973067.3348205/150‑1 (MQ=60)
                                                                                AATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTCGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGGTAAGTGCTTTTTTTTCTGCCCACGCCGTACCGTAAATACGTTGC                                                                   >  SRR19973067.523467/1‑150 (MQ=60)
                                                                                 ATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCA                                                                  <  SRR19973067.3571781/150‑1 (MQ=60)
                                                                                 ATACCAGACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCA                                                                  <  SRR19973067.2880846/150‑1 (MQ=60)
                                                                                  TACCATACCCGGCGCTTCTTCCTGCCTATTGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGGTTCGCGGCTTCTTCCAGGCGCAGCAGGTAAGCGGTAAGTGCTTTTTTGTATGCCCACGCCCTACCCTAAATACCTTGCAA                                                                 >  SRR19973067.536254/1‑150 (MQ=60)
                                                                                  TACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTAGCGGCTTATTCCAGGCGCAGCAGGTAATCGTTAAGTGCTTTTTTTTCTGCCCACGCCGTACCGTAAATAAGTTGCAA                                                                 >  SRR19973067.536128/1‑150 (MQ=60)
                                                                                           CGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTT                                                        <  SRR19973067.86522/150‑1 (MQ=60)
                                                                                           CGGCGCTTCGTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTT                                                        <  SRR19973067.83062/150‑1 (MQ=60)
                                                                                            GGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTG                                                       <  SRR19973067.1580775/150‑1 (MQ=60)
                                                                                            GGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTG                                                       <  SRR19973067.534961/150‑1 (MQ=60)
                                                                                              CGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTT                                                     >  SRR19973067.1292952/1‑150 (MQ=60)
                                                                                              CGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATATTGTTGTT                                                     >  SRR19973067.958437/1‑150 (MQ=60)
                                                                                               GCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTG                                                    <  SRR19973067.1309268/150‑1 (MQ=60)
                                                                                               GCGTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTG                                                    <  SRR19973067.3089450/150‑1 (MQ=60)
                                                                                                 TTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCT                                                  >  SRR19973067.1667038/1‑150 (MQ=60)
                                                                                                 TTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCT                                                  >  SRR19973067.1960264/1‑150 (MQ=60)
                                                                                                    TTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTC                                               <  SRR19973067.2559786/150‑1 (MQ=60)
                                                                                                    TTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTC                                               <  SRR19973067.2559773/150‑1 (MQ=60)
                                                                                                     TCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCG                                              <  SRR19973067.2998128/150‑1 (MQ=60)
                                                                                                     TCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCG                                              <  SRR19973067.2998116/150‑1 (MQ=60)
                                                                                                       CTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCC                                            <  SRR19973067.2547458/150‑1 (MQ=60)
                                                                                                          CATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACG                                         <  SRR19973067.1411772/150‑1 (MQ=60)
                                                                                                          CATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACG                                         >  SRR19973067.2425831/1‑150 (MQ=60)
                                                                                                             ATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCA                                      >  SRR19973067.3149830/1‑150 (MQ=60)
                                                                                                             ATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCA                                      <  SRR19973067.3136005/150‑1 (MQ=60)
                                                                                                             ATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCA                                      >  SRR19973067.3150542/1‑150 (MQ=60)
                                                                                                               GGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGT                                    <  SRR19973067.2044329/150‑1 (MQ=60)
                                                                                                               GGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGT                                    >  SRR19973067.2248089/1‑150 (MQ=60)
                                                                                                               GGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGT                                    >  SRR19973067.2248183/1‑150 (MQ=60)
                                                                                                               GGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGT                                    >  SRR19973067.15854/1‑150 (MQ=60)
                                                                                                                    AGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCC                               >  SRR19973067.3331369/1‑150 (MQ=60)
                                                                                                                      GTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCC                             >  SRR19973067.1525728/1‑150 (MQ=60)
                                                                                                                        CGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTG                           >  SRR19973067.2750342/1‑150 (MQ=60)
                                                                                                                          AGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCC                         <  SRR19973067.3547198/150‑1 (MQ=60)
                                                                                                                           GCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCG                        <  SRR19973067.356008/150‑1 (MQ=60)
                                                                                                                           GCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCG                        <  SRR19973067.356002/150‑1 (MQ=60)
                                                                                                                            CTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGT                       <  SRR19973067.1250391/150‑1 (MQ=60)
                                                                                                                            CTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGT                       >  SRR19973067.1201061/1‑150 (MQ=60)
                                                                                                                            CTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGT                       >  SRR19973067.3010871/1‑150 (MQ=60)
                                                                                                                             TGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTG                      >  SRR19973067.540849/1‑150 (MQ=60)
                                                                                                                               TTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTT                    <  SRR19973067.3247386/150‑1 (MQ=60)
                                                                                                                               TTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTT                    <  SRR19973067.2476182/150‑1 (MQ=60)
                                                                                                                                 TACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTGTTCA                  >  SRR19973067.2894032/1‑150 (MQ=60)
                                                                                                                                      ATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTTCATTAGT             <  SRR19973067.3048605/150‑1 (MQ=60)
                                                                                                                                      ATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTGTTGATTAGT             >  SRR19973067.2122413/1‑150 (MQ=60)
                                                                                                                                       TTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTTCATTAGTT            <  SRR19973067.3188321/150‑1 (MQ=60)
                                                                                                                                        TTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTTCATTAGTTT           <  SRR19973067.301271/150‑1 (MQ=60)
                                                                                                                                        TTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTTCATTAGTTT           <  SRR19973067.1864425/150‑1 (MQ=60)
                                                                                                                                           ACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTTCATTAGTTTGAA        <  SRR19973067.2892357/150‑1 (MQ=60)
                                                                                                                                              GTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTTCATTAGTTTGAAATG     <  SRR19973067.1905538/150‑1 (MQ=60)
                                                                                                                                                GGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTTGATTAGTTTGAAATGAT   >  SRR19973067.171684/1‑150 (MQ=60)
                                                                                                                                                GGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTTCATTAGTTTGTAATGAT   >  SRR19973067.901922/1‑150 (MQ=60)
                                                                                                                                                 GTCGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTTCATTAGTTTGAAATGATG  <  SRR19973067.1567957/150‑1 (MQ=60)
                                                                                                                                                   |                                                                                                                                                   
CCTGATACTGGTACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTCGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTTCATTAGTTTGAAATGATG  >  CP009273/1795954‑1796248

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑