Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,796,101 C→A 94.5% D243Y (GAC→TAC)  thrS ← threonyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,796,1010CA94.5% 537.3 / 17.5 191D243Y (GAC→TAC) thrSthreonyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base C (4/7);  new base A (89/91);  total (93/98)
Fisher's exact test for biased strand distribution p-value = 5.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 194 total aligned reads displayed.

TGATACTGGTACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTCGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTTCATTAGTTTGAAATG  >  CP009273/1795956‑1796245
                                                                                                                                                 |                                                                                                                                                
tGATACTGGTACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTagc                                                                                                                                                >  2:3199119/1‑148 (MQ=255)
 gATACTGGTACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTagcg                                                                                                                                               >  1:217302/1‑148 (MQ=255)
    aCTGGTACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGttt                                                                                                                                            <  2:1016116/148‑1 (MQ=255)
      tGGTACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTcg                                                                                                                                          <  2:1579293/148‑1 (MQ=255)
       ggTACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTcgc                                                                                                                                         >  1:1481677/1‑148 (MQ=255)
         tACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCgg                                                                                                                                       >  2:3429514/1‑148 (MQ=255)
         tACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCgg                                                                                                                                       >  1:3534956/1‑148 (MQ=255)
            tctTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGctt                                                                                                                                    <  2:1828300/148‑1 (MQ=255)
            tctTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCAAATGGTACAGGTCGAGCTGTTTAACGATTTTACGGTGGTAGCGTTTAGCGGctt                                                                                                                                    >  1:2992556/1‑148 (MQ=255)
             ctTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGcttc                                                                                                                                   >  1:554404/1‑148 (MQ=255)
                tCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTCGCGTTTCGCGGCttcttc                                                                                                                                <  1:2469020/148‑1 (MQ=255)
                   gTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAg                                                                                                                             >  2:2129374/1‑148 (MQ=255)
                     ttAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTAACGAATTTACTGTGGTAGCGTTTCGCGGCTTATTACAGgc                                                                                                                           >  2:1985674/1‑148 (MQ=255)
                     ttAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCGTCTTCCTGGATATGGTAAAGGTCGAGCTGTTTAACCATTTTAAGGTGGTAGCGTTTGGCGGCTTGTTCCAGgc                                                                                                                           >  1:3316682/1‑148 (MQ=255)
                      tAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGgcg                                                                                                                          >  2:1010902/1‑148 (MQ=255)
                         aacgaacAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTCCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTg                                                                                                                       >  1:1948175/1‑148 (MQ=255)
                           cgaacAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCa                                                                                                                     <  2:2176135/148‑1 (MQ=255)
                              acaaacaCTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGt                                                                                                                  <  2:597752/148‑1 (MQ=255)
                              acaaacaCTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTAACGATTTTAAGGTGGTATCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGt                                                                                                                  >  1:420373/1‑148 (MQ=255)
                               caaacaCTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCCTATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTa                                                                                                                 >  2:139126/1‑148 (MQ=255)
                                  acacTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGGTGCAGGTAAGc                                                                                                              >  2:2397949/1‑148 (MQ=255)
                                   cacTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTCGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCg                                                                                                             <  2:3007728/148‑1 (MQ=255)
                                   cacTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTCGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCg                                                                                                             <  2:1231411/148‑1 (MQ=255)
                                   cacTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCg                                                                                                             >  1:763965/1‑148 (MQ=255)
                                    acTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGt                                                                                                            <  2:3137617/148‑1 (MQ=255)
                                    acTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGATGCAGGTAAGCGt                                                                                                            <  1:986015/148‑1 (MQ=255)
                                     cTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTGTTCCTTGATATTGTACAGGTCGAGCTGTTTAACGATTTTAAGGTGGTCGCGTTTCGCGGCGTCGTCCCTGCGCTGCAGGGAAGCggt                                                                                                           >  1:190547/1‑146 (MQ=255)
                                     cTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTTCCGATTTTAAGGTGGTAGCGTTTAGCGGCTTCTTCCAGGCGCGGCAGGTAAGCggg                                                                                                           >  1:3280544/1‑146 (MQ=255)
                                      ttCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTa                                                                                                          >  2:169012/1‑148 (MQ=255)
                                      ttCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCGGCATATGGTACAGGTCGAGCTGTTTAACGATTTTAAGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTa                                                                                                          >  2:965658/1‑148 (MQ=255)
                                        ccAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAg                                                                                                        >  2:1158988/1‑148 (MQ=255)
                                          aGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTg                                                                                                      <  2:76004/148‑1 (MQ=255)
                                            ttCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCt                                                                                                    <  2:240852/148‑1 (MQ=255)
                                             tCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCtt                                                                                                   <  2:1798964/148‑1 (MQ=255)
                                               aCGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCtttt                                                                                                 <  1:352834/148‑1 (MQ=255)
                                                cGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCttttt                                                                                                >  2:845785/1‑148 (MQ=255)
                                                 ggAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCCTATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTCGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGGTGAGTGCtttttt                                                                                               >  1:2819984/1‑148 (MQ=255)
                                                 ggAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCtttttt                                                                                               <  1:284288/148‑1 (MQ=255)
                                                  gAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTg                                                                                              <  2:3123952/148‑1 (MQ=255)
                                                  gAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCGGTTTAACGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTTCttttttt                                                                                              >  1:3112194/1‑147 (MQ=255)
                                                   aaGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCttttt                                                                                                >  2:3419126/1‑145 (MQ=255)
                                                   aaGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGt                                                                                             <  2:2727529/148‑1 (MQ=255)
                                                   aaGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGt                                                                                             <  2:2903095/148‑1 (MQ=255)
                                                    aGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTc                                                                                            <  2:989719/148‑1 (MQ=255)
                                                    aGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTc                                                                                            <  2:2009284/148‑1 (MQ=255)
                                                    aGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCAGCATATGGTACAGGTCGAGCTGTTTACAGATTTTAATGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTc                                                                                            >  1:2319416/1‑148 (MQ=255)
                                                     gATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCGTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCt                                                                                           <  2:1104143/148‑1 (MQ=255)
                                                      aTGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTg                                                                                          >  2:1284401/1‑148 (MQ=255)
                                                                     ttGTGCCAGAATACCATACCCGGCGCTTCTTCCGGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGccgtaccgt                                                                           >  2:602997/1‑148 (MQ=255)
                                                                             gAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGt                                                                   <  1:3429514/148‑1 (MQ=255)
                                                                                    ataccCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTCGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACa                                                            <  1:1019954/148‑1 (MQ=255)
                                                                                     taccCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACAt                                                           <  2:2676696/148‑1 (MQ=255)
                                                                                      accCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACAtt                                                          <  2:1781642/148‑1 (MQ=255)
                                                                                      accCGGCGCGTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACAtt                                                          <  1:1911985/148‑1 (MQ=255)
                                                                                            cgcTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTCGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTttgttg                                                    <  2:190547/148‑1 (MQ=255)
                                                                                            cgcTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTttgttg                                                    >  2:3468084/1‑148 (MQ=255)
                                                                                             gcTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTttgttgt                                                   >  2:351798/1‑148 (MQ=255)
                                                                                              cttcttCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTttgttgtt                                                  >  2:2221270/1‑148 (MQ=255)
                                                                                               ttcttcCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTttgttgttg                                                 >  2:1513080/1‑148 (MQ=255)
                                                                                               ttcttcCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTttgttgttg                                                 >  2:1513212/1‑148 (MQ=255)
                                                                                               ttcttcCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTttgttgttg                                                 >  1:2484237/1‑148 (MQ=255)
                                                                                                tcttcCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGc                                                >  2:2825690/1‑148 (MQ=255)
                                                                                                tcttcCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGc                                                >  1:2361325/1‑148 (MQ=255)
                                                                                                   tcCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGt                                             <  1:2657382/148‑1 (MQ=255)
                                                                                                   tcCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGt                                             <  2:3316682/148‑1 (MQ=255)
                                                                                                      tGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTcgc                                          <  1:513328/148‑1 (MQ=255)
                                                                                                      tGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTcgc                                          >  2:649418/1‑148 (MQ=255)
                                                                                                      tGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTcgc                                          >  2:1117405/1‑148 (MQ=255)
                                                                                                      tGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTcgc                                          >  2:1118332/1‑148 (MQ=255)
                                                                                                      tGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGGAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTcgc                                          <  2:2296963/148‑1 (MQ=255)
                                                                                                       gCATATGGTACAGGTCGAGCTGTTTACCGATTATACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTcgcc                                         >  2:3266485/1‑148 (MQ=255)
                                                                                                        cATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTcgcca                                        >  1:3060949/1‑148 (MQ=255)
                                                                                                          tatGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTcgccacg                                      <  1:3162229/148‑1 (MQ=255)
                                                                                                              gTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAg                                  >  2:488740/1‑148 (MQ=255)
                                                                                                               tACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGTTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGt                                 <  1:2397949/148‑1 (MQ=255)
                                                                                                                     tCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGccc                           <  1:2635122/148‑1 (MQ=255)
                                                                                                                     tCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGccc                           <  1:1224191/148‑1 (MQ=255)
                                                                                                                     tCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGccc                           <  1:1114416/148‑1 (MQ=255)
                                                                                                                     tCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGccc                           >  1:267275/1‑148 (MQ=255)
                                                                                                                        aGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTg                        <  1:2166531/148‑1 (MQ=255)
                                                                                                                         gCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGc                       >  1:1707440/1‑148 (MQ=255)
                                                                                                                              ttACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGttt                  >  2:1058509/1‑148 (MQ=255)
                                                                                                                               tACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGtttt                 >  2:1557463/1‑148 (MQ=255)
                                                                                                                                aCCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTTc                <  1:1616661/148‑1 (MQ=255)
                                                                                                                                aCCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTTc                <  2:542543/148‑1 (MQ=255)
                                                                                                                                aCCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGAGTTAAGTGCTTTTTTGTCAGACCACGCCGTACCGTAAATACGATGCAACATTTTGTTGTTGATGTCGCCACGCCAGTAAGCCCATGCCGTTTTc                >  2:2508867/1‑148 (MQ=255)
                                                                                                                                   gATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTTCAtt             >  1:2730392/1‑148 (MQ=255)
                                                                                                                                     ttttACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTTCATTAg           >  2:1868003/1‑148 (MQ=255)
                                                                                                                                     ttttACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTTCATTAg           <  1:847094/148‑1 (MQ=255)
                                                                                                                                      tttACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTTGATTAGt          >  1:3391702/1‑148 (MQ=255)
                                                                                                                                       ttACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTTCATTAGtt         <  2:532520/148‑1 (MQ=255)
                                                                                                                                         aCGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTTCATTAGTTTg       <  2:792521/148‑1 (MQ=255)
                                                                                                                                          cGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTTCATTAGTTTGa      <  1:1284401/148‑1 (MQ=255)
                                                                                                                                          cGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTGTTGATTAGTTTgt      >  1:2138215/1‑147 (MQ=255)
                                                                                                                                           ggtggtAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTTCATTAGTTTGaa     <  2:3511178/148‑1 (MQ=255)
                                                                                                                                            gtggtAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTTCATTAGTTTGaaa    <  2:2899667/148‑1 (MQ=255)
                                                                                                                                            gtggtAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTTCATTAGTTTGaaa    <  2:1917615/148‑1 (MQ=255)
                                                                                                                                             tggtAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTTCATTAGTTTGAAat   >  2:2651769/1‑148 (MQ=255)
                                                                                                                                              ggtAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTTCATTAGTTTGAAatg  <  2:555716/148‑1 (MQ=255)
                                                                                                                                              ggtAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTTCATTAGTTTGAAatg  <  2:3386589/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TGATACTGGTACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTCGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTTCATTAGTTTGAAATG  >  CP009273/1795956‑1796245

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCTGATACTGGTACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTCGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTTCATTAGTTTGAAATGAT  >  CP009273/1795954‑1796247
                                                                                                                                                   |                                                                                                                                                  
CCTGATACTGGTACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGC                                                                                                                                                  >  SRR19973066.3156866/1‑150 (MQ=60)
 CTGATACTGGTACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCG                                                                                                                                                 >  SRR19973066.219086/1‑150 (MQ=60)
     TACTGGTACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTC                                                                                                                                             <  SRR19973066.2446852/150‑1 (MQ=60)
      ACTGGTACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCG                                                                                                                                            <  SRR19973066.3332830/150‑1 (MQ=60)
      ACTGGTACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCG                                                                                                                                            <  SRR19973066.704646/150‑1 (MQ=60)
       CTGGTACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGC                                                                                                                                           >  SRR19973066.1492087/1‑150 (MQ=60)
         GGTACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGG                                                                                                                                         >  SRR19973066.3558462/1‑150 (MQ=60)
            ACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCAAATGGTACAGGTCGAGCTGTTTAACGATTTTACGGTGGTAGCGTTTAGCGGCTT                                                                                                                                      >  SRR19973066.3012387/1‑150 (MQ=60)
             CTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTC                                                                                                                                     >  SRR19973066.558381/1‑150 (MQ=60)
             CTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTC                                                                                                                                     >  SRR19973066.1476658/1‑150 (MQ=60)
                  TCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTCGCGTTTCGCGGCTTCTTCCA                                                                                                                                <  SRR19973066.2485857/150‑1 (MQ=60)
                     GTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTAACGATTTTAAGGTGGTAGCGTTTAGCGGCTTATTACAGGC                                                                                                                             >  SRR19973066.1277396/1‑150 (MQ=60)
                     GTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCAGCATATGGTACATGTCGAGGTGTTTTCCGATTTTTCGGTGGTAGCGTTTAGCGGCTTCTTACAGGC                                                                                                                             >  SRR19973066.1277391/1‑150 (MQ=60)
                     GTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCGTCTTCCTGGATATGGTAAAGGTCGAGCTGTTTAACCATTTTAAGGTGGTAGCGTTTGGCGGCTTGTTCCAGGC                                                                                                                             >  SRR19973066.3338702/1‑150 (MQ=60)
                         AGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTCCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTG                                                                                                                         >  SRR19973066.1961975/1‑150 (MQ=60)
                              GAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTAAGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGT                                                                                                                    >  SRR19973066.2962875/1‑150 (MQ=60)
                              GAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTAACGATTTTAAGGTGGTATCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGT                                                                                                                    >  SRR19973066.423645/1‑150 (MQ=60)
                                 CAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTATCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAG                                                                                                                 >  SRR19973066.2182572/1‑150 (MQ=60)
                                   AACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCGTCTTCCAGGCGCTGCAGGTAAGCG                                                                                                               >  SRR19973066.546471/1‑150 (MQ=60)
                                   AACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTAGCGGCTTCTTCCAGGCGCTGCAGGTAAGCG                                                                                                               >  SRR19973066.769630/1‑150 (MQ=60)
                                     CACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTGTTCCTTGATATTGTACAGGTCGAGCTGTTTAACGATTTTAAGGTGGTCGCGTTTCGCGGCGTCGTCCCTGCGCTGCAGGGAAGCGGT                                                                                                             >  SRR19973066.192192/1‑150 (MQ=60)
                                     CACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTTCCGATTTTAAGGTGGTAGCGTTTAGCGGCTTCTTCCAGGCGCGGCAGGTAAGCGgg                                                                                                             >  SRR19973066.3302103/1‑148 (MQ=60)
                                     CACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTCGCGTTTCGCGGCTTCTTCCAGGCGCTGCCGGTAAGCGTT                                                                                                             >  SRR19973066.192195/1‑150 (MQ=60)
                                     CACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCCGGTAAGCGTT                                                                                                             >  SRR19973066.3410391/1‑150 (MQ=60)
                                     CACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTAACGATTTTAAGGTGGTCGCGTTTCGCGGCGTCTTCCACGCGCTTCAGGTAAGCGTT                                                                                                             >  SRR19973066.192076/1‑150 (MQ=60)
                                      ACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGATGCAGGTAAGCGTTA                                                                                                            <  SRR19973066.993286/150‑1 (MQ=60)
                                           CAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTTAGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGC                                                                                                       >  SRR19973066.2562031/1‑150 (MQ=60)
                                               TCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTT                                                                                                   <  SRR19973066.1831857/150‑1 (MQ=60)
                                                 ACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCCTATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTCGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGGTGAGTGCTTTTTT                                                                                                 >  SRR19973066.2838935/1‑150 (MQ=60)
                                                 ACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTT                                                                                                 <  SRR19973066.2333327/150‑1 (MQ=60)
                                                 ACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTT                                                                                                 <  SRR19973066.355584/150‑1 (MQ=60)
                                                 ACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTT                                                                                                 <  SRR19973066.355616/150‑1 (MQ=60)
                                                  TGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTG                                                                                                <  SRR19973066.3441999/150‑1 (MQ=60)
                                                  CGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCGGTTTAACGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTTCTTTTTTT                                                                                                >  SRR19973066.3132980/1‑150 (MQ=60)
                                                   GGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATTGTACAGGTCGAGCTGTTTACCGAATTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGGTAAGTGCTTTTTTGT                                                                                               >  SRR19973066.1602922/1‑150 (MQ=60)
                                                   GGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGT                                                                                               <  SRR19973066.286343/150‑1 (MQ=60)
                                                   GGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGT                                                                                               <  SRR19973066.286337/150‑1 (MQ=60)
                                                    GAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCAGCATATGGTACAGGTCGAGCTGTTTACAGATTTTAATGTGGTAGCGTTTCGCGGCTTCTTACAGGCGCTGCAGGTAAGCGGTAAGTGCTTTTTTGTC                                                                                              >  SRR19973066.2335269/1‑150 (MQ=60)
                                                           GTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTACATCATATGGTACACGTCGAGCTGTTTACCCATTTTAAGGTGGTAGCGTTTAGCGGCTTCTTACAGGCGCTGCAGGTAAGCGTTAAGTTCTTTTTTTTGTGCCCAC                                                                                       >  SRR19973066.2745261/1‑150 (MQ=60)
                                                             CCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATTGTACAGGTCGAGCTGTTTAACGATTTTAAGGGGGTAGCGTTTAGCGGGTTCTTACAGGCGCTGCCGGTAAGCGTTAAGTGCTTTTTTGTCTTCCCACCC                                                                                     >  SRR19973066.2412871/1‑150 (MQ=60)
                                                                GCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGGTAAGTGCTTTTTTGTCTGCCCACGCCGT                                                                                  >  SRR19973066.2676986/1‑150 (MQ=60)
                                                                 CCGTCGTTGTGCCAGAATACCATACCCGGCGCTTGTTCCTGCATATGGTACAGGTCGAGCGGTTTACCGATTTTACGGTGGTAGCGTTTAGCGGCGTCTTCCAGGCGCTGCCGGTAAGCGTTAAGTGCTTTTTTTTCTGCCCACGCCGTA                                                                                 >  SRR19973066.3114125/1‑150 (MQ=60)
                                                                     CGTTGTGCCAGAATACCATACCCGGCGCGTGTTCCTGCCTATGGTACAGGTCGAGCTGTTTACCCATTTTTAGGTGGTAGCGGTTCGCGGCTTCTTCCGGGCGCTGGAGGTAAGCGTTAAGTGCTTTTTTTTCTTCCCACGCCGTACCGG                                                                             >  SRR19973066.3440447/1‑150 (MQ=60)
                                                                              AGAATACCATCCCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTT                                                                    <  SRR19973066.3027872/150‑1 (MQ=60)
                                                                              AGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTCGCGTTTCGCGGCTTCTTCCAGGCGCTGCCGGTAAGCGGGAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTT                                                                    >  SRR19973066.2812413/1‑150 (MQ=60)
                                                                               GAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTG                                                                   <  SRR19973066.3452450/150‑1 (MQ=60)
                                                                                      ATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTCGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATT                                                            <  SRR19973066.1027457/150‑1 (MQ=60)
                                                                                        ACCCGGCGCGTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTT                                                          <  SRR19973066.1925600/150‑1 (MQ=60)
                                                                                         CCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTG                                                         <  SRR19973066.911064/150‑1 (MQ=60)
                                                                                            GGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTG                                                      >  SRR19973066.466435/1‑150 (MQ=60)
                                                                                               GCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTG                                                   >  SRR19973066.2501185/1‑150 (MQ=60)
                                                                                                CTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGC                                                  >  SRR19973066.2377329/1‑150 (MQ=60)
                                                                                                     TCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCG                                             <  SRR19973066.2675203/150‑1 (MQ=60)
                                                                                                        TGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCA                                          >  SRR19973066.3081424/1‑150 (MQ=60)
                                                                                                        TGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCA                                          <  SRR19973066.517119/150‑1 (MQ=60)
                                                                                                          CATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACG                                        >  SRR19973066.3275614/1‑150 (MQ=60)
                                                                                                          CATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACG                                        <  SRR19973066.1138181/150‑1 (MQ=60)
                                                                                                           ATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGC                                       >  SRR19973066.1930189/1‑150 (MQ=60)
                                                                                                            TATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCC                                      <  SRR19973066.3183293/150‑1 (MQ=60)
                                                                                                             ATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCA                                     <  SRR19973066.2670745/150‑1 (MQ=60)
                                                                                                                 TACAGGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGTTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAA                                 <  SRR19973066.2414118/150‑1 (MQ=60)
                                                                                                                    AGGTCGAGCTGTTTACCGATTTGACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCC                              <  SRR19973066.2375732/150‑1 (MQ=60)
                                                                                                                     GGTCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCC                             >  SRR19973066.269267/1‑150 (MQ=60)
                                                                                                                       TCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCT                           <  SRR19973066.1232855/150‑1 (MQ=60)
                                                                                                                       TCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCT                           <  SRR19973066.1122460/150‑1 (MQ=60)
                                                                                                                       TCGAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCT                           <  SRR19973066.2652865/150‑1 (MQ=60)
                                                                                                                         GAGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGC                         >  SRR19973066.1719830/1‑150 (MQ=60)
                                                                                                                          AGCTGTTTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCC                        <  SRR19973066.2181578/150‑1 (MQ=60)
                                                                                                                                TTACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTTC                  <  SRR19973066.805017/150‑1 (MQ=60)
                                                                                                                                  ACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTTCAT                <  SRR19973066.1629572/150‑1 (MQ=60)
                                                                                                                                  ACCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTTCAT                <  SRR19973066.1628390/150‑1 (MQ=60)
                                                                                                                                   CCGATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTTAATT               >  SRR19973066.2748755/1‑150 (MQ=60)
                                                                                                                                    CGTTTTTACGGTGGTAGCGTTTCGCGGCTTCTTCGAGGCGCTGCATTTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTATATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTTCATTA              <  SRR19973066.1999673/150‑1 (MQ=60)
                                                                                                                                      ATTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTTGATTATT            >  SRR19973066.3414337/1‑150 (MQ=60)
                                                                                                                                       TTTTACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTTCATTAGTT           <  SRR19973066.853234/150‑1 (MQ=60)
                                                                                                                                          TACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTGTTGATTAGTTTGT        >  SRR19973066.2153047/1‑150 (MQ=60)
                                                                                                                                          TACGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTGTTGATTAGTTTGT        >  SRR19973066.2153842/1‑150 (MQ=60)
                                                                                                                                            CGGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTTCATTAGTTTGAAA      <  SRR19973066.1293677/150‑1 (MQ=60)
                                                                                                                                             GGTGGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTTCATTAGTTTGAAAT     <  SRR19973066.3547365/150‑1 (MQ=60)
                                                                                                                                                GGTAGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTGTTCATTAGTTTGTAAAGAT  >  SRR19973066.1695608/1‑150 (MQ=60)
                                                                                                                                                   |                                                                                                                                                  
CCTGATACTGGTACTCTTTCAGTTTAGAACGAACAAACACTTCCAGTTCACGGAAGATGGTCCAGCCGTCGTTGTGCCAGAATACCATACCCGGCGCTTCTTCCTGCATATGGTACAGGTCGAGCTGTTTACCGATTTTACGGTGGTCGCGTTTCGCGGCTTCTTCCAGGCGCTGCAGGTAAGCGTTAAGTGCTTTTTTGTCTGCCCACGCCGTACCGTAAATACGTTGCAACATTTTGTTGTTGCTGTCGCCACGCCAGTAAGCCCCTGCCGTTTTCATTAGTTTGAAATGAT  >  CP009273/1795954‑1796247

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑