Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,800,129 C→T 100% W71* (TGG→TAG)  ydiY ← acid‑inducible putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,800,1290CT98.4% 819.0 / 2.2 251W71* (TGG→TAG) ydiYacid‑inducible putative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base C (2/2);  new base T (110/137);  total (112/139)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 251 total aligned reads displayed.

GCTTGCCTGACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTG  >  CP009273/1799984‑1800273
                                                                                                                                                 |                                                                                                                                                
gcTTGCCTTACAAAATAAATCTTATTAATCCTTTAAATAAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACCGCGACCAGGCAGTGGTGTGTCCATACTAg                                                                                                                                                <  1:2081701/148‑1 (MQ=255)
   tGCCTGACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTc                                                                                                                                             >  1:2290653/1‑148 (MQ=255)
     ccTGACCAAATAAATAGTCATAATCAGTTAAATTAACACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCAt                                                                                                                                           <  1:3499669/148‑1 (MQ=255)
         aCCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCAtggtg                                                                                                                                       >  2:3426937/1‑148 (MQ=255)
         aCCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCAtggtg                                                                                                                                       <  1:1726057/148‑1 (MQ=255)
         aCCAAATAAATACTCATAATCAGTTTAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTTAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCAtggtg                                                                                                                                       <  1:1427642/148‑1 (MQ=255)
          ccAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCAtggtgg                                                                                                                                      >  1:3224543/1‑148 (MQ=255)
          ccAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCAtggtgg                                                                                                                                      >  2:857819/1‑148 (MQ=255)
           cAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATggtggt                                                                                                                                     >  1:2747350/1‑148 (MQ=255)
            aaataaatAGTCATAATCAGTTAAATTAACACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGgtggtg                                                                                                                                    <  1:2493024/148‑1 (MQ=255)
             aataaataGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGgtgt                                                                                                                                   <  1:570464/148‑1 (MQ=255)
             aataaataGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGgtgt                                                                                                                                   >  2:2869296/1‑148 (MQ=255)
              ataaataGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTc                                                                                                                                  <  1:684173/148‑1 (MQ=255)
              ataaataGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTc                                                                                                                                  >  2:169427/1‑148 (MQ=255)
              ataaagaGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTc                                                                                                                                  <  2:2244246/148‑1 (MQ=255)
                aaataGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAg                                                                                                                                >  1:271578/1‑148 (MQ=255)
                 aataGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGc                                                                                                                               >  2:2760540/1‑148 (MQ=255)
                   taGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCg                                                                                                                             >  2:45730/1‑148 (MQ=255)
                   taGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCAGCATTAGAAGAAGTAGTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCg                                                                                                                             <  2:791193/148‑1 (MQ=255)
                    aGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGt                                                                                                                            >  1:2591015/1‑148 (MQ=255)
                    aGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGt                                                                                                                            >  1:264000/1‑148 (MQ=255)
                     gTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTa                                                                                                                           <  2:172665/148‑1 (MQ=255)
                       cATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGGTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAg                                                                                                                         <  2:3035872/148‑1 (MQ=255)
                         tAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTg                                                                                                                       <  2:135322/148‑1 (MQ=255)
                         tAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTg                                                                                                                       >  2:2527032/1‑148 (MQ=255)
                          aaTCAGTTAAATTAACACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTga                                                                                                                      <  1:948620/148‑1 (MQ=255)
                          aaTCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTga                                                                                                                      <  1:2533096/148‑1 (MQ=255)
                          aaTCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTga                                                                                                                      >  1:685482/1‑148 (MQ=255)
                            tCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTgagg                                                                                                                    >  1:1651419/1‑148 (MQ=255)
                             cAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTgagga                                                                                                                   >  2:2218097/1‑148 (MQ=255)
                               gtgaaattacaACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGc                                                                                                                 <  2:945338/145‑1 (MQ=255)
                               gTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGc                                                                                                                 <  2:643758/148‑1 (MQ=255)
                               gTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGc                                                                                                                 <  2:1406760/148‑1 (MQ=255)
                                ttaaattaaaACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCt                                                                                                                >  1:2867454/1‑148 (MQ=255)
                                  aaatgaaaACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCttt                                                                                                              <  2:2311753/148‑1 (MQ=255)
                                   aattaaaACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCtttt                                                                                                             <  1:2567823/148‑1 (MQ=255)
                                     ttaaaACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCg                                                                                                           >  2:1951757/1‑148 (MQ=255)
                                      taaaACGGCTACGCCAGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGt                                                                                                          >  2:2556433/1‑148 (MQ=255)
                                       aacaCGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTa                                                                                                         <  1:771094/145‑1 (MQ=255)
                                        aaaCGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTAATGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTAt                                                                                                        <  1:3148086/148‑1 (MQ=255)
                                         aaCGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTAtt                                                                                                       >  1:1083792/1‑148 (MQ=255)
                                           cGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGc                                                                                                     <  2:2935643/148‑1 (MQ=255)
                                            ggCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGcc                                                                                                    <  1:2947709/148‑1 (MQ=255)
                                              cTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTg                                                                                                  <  2:3402601/148‑1 (MQ=255)
                                                 cgcccgcccgccGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGAtt                                                                                               >  2:3265806/1‑148 (MQ=255)
                                                   cccgcccgccGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATtgt                                                                                             >  2:1515784/1‑148 (MQ=255)
                                                         cgccgcATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGa                                                                                       <  1:2519133/148‑1 (MQ=255)
                                                          gccgcATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGat                                                                                      <  2:2462048/148‑1 (MQ=255)
                                                            cgcATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGatat                                                                                    <  1:332807/148‑1 (MQ=255)
                                                             gcATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATc                                                                                   <  2:2221606/148‑1 (MQ=255)
                                                             gcATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATc                                                                                   >  2:83527/1‑148 (MQ=255)
                                                             gcATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATc                                                                                   <  1:202928/148‑1 (MQ=255)
                                                              cATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATcc                                                                                  <  1:1534295/148‑1 (MQ=255)
                                                                  tttttCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCg                                                                              >  2:1966133/1‑148 (MQ=255)
                                                                      tCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCa                                                                          <  2:1252372/148‑1 (MQ=255)
                                                                        tGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGa                                                                        <  2:137406/148‑1 (MQ=255)
                                                                            gaaCGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTAc                                                                    >  2:248530/1‑148 (MQ=255)
                                                                             aaCGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTAcc                                                                   <  1:3272418/148‑1 (MQ=255)
                                                                             aaCGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTAcc                                                                   <  1:1265163/148‑1 (MQ=255)
                                                                             aaCGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTAcc                                                                   <  2:3113635/148‑1 (MQ=255)
                                                                              aCGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCt                                                                  <  2:156852/148‑1 (MQ=255)
                                                                                gCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTc                                                                >  2:2974847/1‑148 (MQ=255)
                                                                                   catcatTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGaa                                                             >  1:1302372/1‑148 (MQ=255)
                                                                                    atcatTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGaaa                                                            <  2:1317062/148‑1 (MQ=255)
                                                                                     tcatTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAg                                                           <  1:1918670/148‑1 (MQ=255)
                                                                                      catTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAgg                                                          >  2:1952379/1‑148 (MQ=255)
                                                                                      catTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAgg                                                          >  2:622755/1‑148 (MQ=255)
                                                                                          agaagaAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGtttt                                                      <  1:965412/148‑1 (MQ=255)
                                                                                                 gTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAgcgc                                               <  2:712608/148‑1 (MQ=255)
                                                                                                  tATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCt                                              <  2:2078490/148‑1 (MQ=255)
                                                                                                    ttACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTgg                                            >  1:2457543/1‑148 (MQ=255)
                                                                                                     tACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGc                                           >  1:2317887/1‑148 (MQ=255)
                                                                                                       cTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAg                                         >  1:929080/1‑148 (MQ=255)
                                                                                                         ggCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGa                                       <  1:3161636/148‑1 (MQ=255)
                                                                                                          gCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGat                                      <  1:2799483/148‑1 (MQ=255)
                                                                                                             ttCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGatcat                                   >  1:1138473/1‑148 (MQ=255)
                                                                                                              tCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGatcatc                                  >  2:3487326/1‑148 (MQ=255)
                                                                                                              tCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGatcatc                                  >  2:1428856/1‑148 (MQ=255)
                                                                                                               cccccACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATcc                                 <  2:2682793/148‑1 (MQ=255)
                                                                                                                ccccACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCa                                >  1:1417591/1‑148 (MQ=255)
                                                                                                                    acaGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGaa                            >  2:2113864/1‑147 (MQ=255)
                                                                                                                    acaGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGAc                            <  2:2056424/148‑1 (MQ=255)
                                                                                                                    acaGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTGTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGAc                            <  2:1043372/148‑1 (MQ=255)
                                                                                                                     caGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACa                           <  1:3393189/148‑1 (MQ=255)
                                                                                                                         gACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTaa                       <  1:2970805/148‑1 (MQ=255)
                                                                                                                          aCCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTaaa                      >  2:2501699/1‑148 (MQ=255)
                                                                                                                              ggCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACg                  <  2:239861/148‑1 (MQ=255)
                                                                                                                               gCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACgg                 >  1:2428717/1‑148 (MQ=255)
                                                                                                                                cAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGa                >  1:385636/1‑148 (MQ=255)
                                                                                                                                cAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGa                <  1:2652030/148‑1 (MQ=255)
                                                                                                                                 aGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGaa               <  1:3086633/148‑1 (MQ=255)
                                                                                                                                 aGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGaa               <  2:1842990/148‑1 (MQ=255)
                                                                                                                                  gtggtgTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAat              <  2:3224543/148‑1 (MQ=255)
                                                                                                                                  gtggtgTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAat              <  1:1952375/148‑1 (MQ=255)
                                                                                                                                   tggtgTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAatc             <  1:80247/148‑1 (MQ=255)
                                                                                                                                   tggtgTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAatc             <  1:169438/148‑1 (MQ=255)
                                                                                                                                    ggtgTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAatca            <  2:2943582/148‑1 (MQ=255)
                                                                                                                                           ccATTCTAGTTCATGGTGGTGTCAGCCGTATGTGTGGAGCTTTTCTTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTAACTTCGTATGGTTTTTTAGCGCTGGCAGGATCTTCCATGAGAGTAAAAACGGAATCATCGGCAg     <  2:1704438/148‑1 (MQ=255)
                                                                                                                                           ccATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAg     >  1:2422526/1‑148 (MQ=255)
                                                                                                                                             aTACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCt   >  1:820175/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GCTTGCCTGACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTG  >  CP009273/1799984‑1800273

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCAGCTTGCCTGACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTC  >  CP009273/1799981‑1800278
                                                                                                                                                    |                                                                                                                                                     
CCAGCTTGCCTGACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTA                                                                                                                                                      >  SRR19973067.1291893/1‑150 (MQ=60)
 CAGCTTGCCTGACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAG                                                                                                                                                     >  SRR19973067.810899/1‑150 (MQ=60)
   gcttgccttacaaaataaatcttattaatcctTTAAATAAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACCGCGACCAGGCAGTGGTGTGTCCATACTAGGT                                                                                                                                                   <  SRR19973067.2095496/118‑1 (MQ=60)
   GCTTGCCTGACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGT                                                                                                                                                   <  SRR19973067.2276790/150‑1 (MQ=60)
    CTTGCCTGACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTC                                                                                                                                                  >  SRR19973067.2306338/1‑150 (MQ=60)
    CTTGCCTGACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTC                                                                                                                                                  >  SRR19973067.2305788/1‑150 (MQ=60)
     TTGCCTGACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCA                                                                                                                                                 >  SRR19973067.1694562/1‑150 (MQ=60)
       GCCTGACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATG                                                                                                                                               <  SRR19973067.67356/150‑1 (MQ=60)
        CCTGACCAAATAAATAGTCATAATCAGTTAAATTAACACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGG                                                                                                                                              <  SRR19973067.3521861/150‑1 (MQ=60)
           GACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGG                                                                                                                                           >  SRR19973067.3244801/1‑150 (MQ=60)
            ACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGT                                                                                                                                          <  SRR19973067.1737885/150‑1 (MQ=60)
            ACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGT                                                                                                                                          >  SRR19973067.2765105/1‑150 (MQ=60)
            ACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGT                                                                                                                                          <  SRR19973067.1436457/150‑1 (MQ=60)
            ACCAAATAAATACTCATAATCAGTTTAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTTAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGT                                                                                                                                          <  SRR19973067.1437273/150‑1 (MQ=60)
             CCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTG                                                                                                                                         >  SRR19973067.3246124/1‑150 (MQ=60)
               AAATAAATAGTCATAATCAGTTAAATTAACACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTC                                                                                                                                       <  SRR19973067.2509402/150‑1 (MQ=60)
                AATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCA                                                                                                                                      <  SRR19973067.574161/150‑1 (MQ=60)
                 ATAAGTAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAG                                                                                                                                     <  SRR19973067.2829135/150‑1 (MQ=60)
                 ATAAATCGTAATAATCATTTAAATTAACCCGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGATGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAG                                                                                                                                     <  SRR19973067.688888/150‑1 (MQ=60)
                 ATAAATATTAATAATCATTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAG                                                                                                                                     <  SRR19973067.688869/150‑1 (MQ=60)
                 ATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAG                                                                                                                                     >  SRR19973067.273394/1‑150 (MQ=60)
                     ATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGT                                                                                                                                 >  SRR19973067.265793/1‑150 (MQ=60)
                     ATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGT                                                                                                                                 >  SRR19973067.2607911/1‑150 (MQ=60)
                     ATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGT                                                                                                                                 >  SRR19973067.2607880/1‑150 (MQ=60)
                         TCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGT                                                                                                                             >  SRR19973067.2986227/1‑150 (MQ=60)
                           ATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGA                                                                                                                           >  SRR19973067.1326054/1‑150 (MQ=60)
                           ATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGA                                                                                                                           >  SRR19973067.690194/1‑150 (MQ=60)
                             AATCAGTTAAATTAACACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGG                                                                                                                         <  SRR19973067.954977/150‑1 (MQ=60)
                             AATCAGTTAAATTAACACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGG                                                                                                                         <  SRR19973067.955110/150‑1 (MQ=60)
                             AATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGG                                                                                                                         <  SRR19973067.2549693/150‑1 (MQ=60)
                             AATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGG                                                                                                                         >  SRR19973067.2701231/1‑150 (MQ=60)
                             AATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGG                                                                                                                         >  SRR19973067.911281/1‑150 (MQ=60)
                             AATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGG                                                                                                                         >  SRR19973067.1662792/1‑150 (MQ=60)
                             AATCAGTTAAATAAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGG                                                                                                                         <  SRR19973067.955060/150‑1 (MQ=60)
                                CAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGC                                                                                                                      <  SRR19973067.1175357/150‑1 (MQ=60)
                                 AGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCT                                                                                                                     >  SRR19973067.2885818/1‑150 (MQ=60)
                                 AGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCT                                                                                                                     >  SRR19973067.1945857/1‑150 (MQ=60)
                                  GTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTT                                                                                                                    >  SRR19973067.158165/1‑150 (MQ=60)
                                      AATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCG                                                                                                                <  SRR19973067.2583087/150‑1 (MQ=60)
                                      AATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCG                                                                                                                <  SRR19973067.2584585/150‑1 (MQ=60)
                                          AACACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATT                                                                                                            <  SRR19973067.776439/150‑1 (MQ=60)
                                          AAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATT                                                                                                            <  SRR19973067.776330/150‑1 (MQ=60)
                                          AAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATT                                                                                                            >  SRR19973067.1091206/1‑150 (MQ=60)
                                           AAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTG                                                                                                           >  SRR19973067.2596962/1‑150 (MQ=60)
                                           AAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTAATGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTG                                                                                                           <  SRR19973067.3168061/150‑1 (MQ=60)
                                               GGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTG                                                                                                       <  SRR19973067.2809887/150‑1 (MQ=60)
                                               GGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTG                                                                                                       >  SRR19973067.709060/1‑150 (MQ=60)
                                               GGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTG                                                                                                       <  SRR19973067.2966366/150‑1 (MQ=60)
                                                  TACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATT                                                                                                    <  SRR19973067.1452745/150‑1 (MQ=60)
                                                  TACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATT                                                                                                    >  SRR19973067.1855561/1‑150 (MQ=60)
                                                      CCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGC                                                                                                <  SRR19973067.3267374/150‑1 (MQ=60)
                                                         GCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAG                                                                                             <  SRR19973067.2415415/150‑1 (MQ=60)
                                                          CCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGA                                                                                            >  SRR19973067.1101018/1‑150 (MQ=60)
                                                          CCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGA                                                                                            >  SRR19973067.3554475/1‑150 (MQ=60)
                                                            CGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATA                                                                                          >  SRR19973067.1249672/1‑150 (MQ=60)
                                                            CGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATA                                                                                          <  SRR19973067.2535650/150‑1 (MQ=60)
                                                            CGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATA                                                                                          <  SRR19973067.2534911/150‑1 (MQ=60)
                                                              CCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATC                                                                                        >  SRR19973067.1734807/1‑150 (MQ=60)
                                                               CGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCC                                                                                       <  SRR19973067.335080/150‑1 (MQ=60)
                                                               CGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCC                                                                                       <  SRR19973067.332470/150‑1 (MQ=60)
                                                                GCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCT                                                                                      <  SRR19973067.204470/150‑1 (MQ=60)
                                                                 CATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTG                                                                                     <  SRR19973067.1544675/150‑1 (MQ=60)
                                                                     TTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTT                                                                                 <  SRR19973067.3012333/150‑1 (MQ=60)
                                                                       TTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCA                                                                               <  SRR19973067.2896309/150‑1 (MQ=60)
                                                                       TTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCA                                                                               <  SRR19973067.2896086/150‑1 (MQ=60)
                                                                        TTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAG                                                                              >  SRR19973067.958335/1‑150 (MQ=60)
                                                                           TGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATT                                                                           <  SRR19973067.2578683/150‑1 (MQ=60)
                                                                              AGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACC                                                                        <  SRR19973067.596452/150‑1 (MQ=60)
                                                                                AACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTT                                                                      <  SRR19973067.1273749/150‑1 (MQ=60)
                                                                                AACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTT                                                                      <  SRR19973067.3292869/150‑1 (MQ=60)
                                                                                 ACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTC                                                                     >  SRR19973067.2438602/1‑150 (MQ=60)
                                                                                 ACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTC                                                                     >  SRR19973067.249976/1‑150 (MQ=60)
                                                                                    CTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAA                                                                  >  SRR19973067.1313269/1‑150 (MQ=60)
                                                                                    CTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAA                                                                  >  SRR19973067.1311255/1‑150 (MQ=60)
                                                                                      CATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAG                                                                >  SRR19973067.1897665/1‑150 (MQ=60)
                                                                                       ATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCCGATTACCTTCGAAAGG                                                               <  SRR19973067.3413885/150‑1 (MQ=60)
                                                                                        TCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGT                                                              <  SRR19973067.1931760/150‑1 (MQ=60)
                                                                                            TAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTT                                                          <  SRR19973067.3111148/150‑1 (MQ=60)
                                                                                             AGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTT                                                         <  SRR19973067.972027/150‑1 (MQ=60)
                                                                                             AGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTT                                                         <  SRR19973067.2759220/150‑1 (MQ=60)
                                                                                              GAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTA                                                        >  SRR19973067.820656/1‑150 (MQ=60)
                                                                                               AAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAG                                                       <  SRR19973067.3448746/150‑1 (MQ=60)
                                                                                               AAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAG                                                       <  SRR19973067.2543598/150‑1 (MQ=60)
                                                                                                     TATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGG                                                 >  SRR19973067.2473700/1‑150 (MQ=60)
                                                                                                      ATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGC                                                >  SRR19973067.2333131/1‑150 (MQ=60)
                                                                                                        TACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAG                                              >  SRR19973067.935359/1‑150 (MQ=60)
                                                                                                            GGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATC                                          <  SRR19973067.3181579/150‑1 (MQ=60)
                                                                                                            GGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATC                                          <  SRR19973067.3181663/150‑1 (MQ=60)
                                                                                                            GGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATC                                          <  SRR19973067.3181754/150‑1 (MQ=60)
                                                                                                             GCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCA                                         <  SRR19973067.2817464/150‑1 (MQ=60)
                                                                                                             GCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCA                                         <  SRR19973067.2817542/150‑1 (MQ=60)
                                                                                                              CGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCAT                                        >  SRR19973067.1146132/1‑150 (MQ=60)
                                                                                                              CGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCAT                                        <  SRR19973067.3366360/150‑1 (MQ=60)
                                                                                                                TTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCC                                      <  SRR19973067.412899/150‑1 (MQ=60)
                                                                                                                 TCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCA                                     >  SRR19973067.1427177/1‑150 (MQ=60)
                                                                                                                   CCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATG                                   <  SRR19973067.2076005/150‑1 (MQ=60)
                                                                                                                        CAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGT                              >  SRR19973067.1293306/1‑150 (MQ=60)
                                                                                                                        CAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGT                              >  SRR19973067.3077031/1‑150 (MQ=60)
                                                                                                                        CAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGT                              <  SRR19973067.3414775/150‑1 (MQ=60)
                                                                                                                         AGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTA                             <  SRR19973067.2223692/150‑1 (MQ=60)
                                                                                                                         AGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTA                             >  SRR19973067.2392308/1‑150 (MQ=60)
                                                                                                                            GACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAA                          <  SRR19973067.2989549/150‑1 (MQ=60)
                                                                                                                            GACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAA                          >  SRR19973067.2660451/1‑150 (MQ=60)
                                                                                                                                AGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGG                      >  SRR19973067.2446932/1‑150 (MQ=60)
                                                                                                                                AGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGG                      >  SRR19973067.2444664/1‑150 (MQ=60)
                                                                                                                                AGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGG                      >  SRR19973067.2444659/1‑150 (MQ=60)
                                                                                                                                 GGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGA                     >  SRR19973067.3528037/1‑150 (MQ=60)
                                                                                                                                 GGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGA                     >  SRR19973067.388327/1‑150 (MQ=60)
                                                                                                                                   CAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAAT                   <  SRR19973067.2669124/150‑1 (MQ=60)
                                                                                                                                    AGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATC                  <  SRR19973067.3106269/150‑1 (MQ=60)
                                                                                                                                    AGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATC                  >  SRR19973067.3173773/1‑150 (MQ=60)
                                                                                                                                     GTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCA                 <  SRR19973067.1965668/150‑1 (MQ=60)
                                                                                                                                     GTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCA                 <  SRR19973067.1965664/150‑1 (MQ=60)
                                                                                                                                      TGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCAT                >  SRR19973067.2515782/1‑150 (MQ=60)
                                                                                                                                      TGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCAT                <  SRR19973067.81139/150‑1 (MQ=60)
                                                                                                                                      TGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCAT                <  SRR19973067.170817/150‑1 (MQ=60)
                                                                                                                                      TGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCAT                <  SRR19973067.170806/150‑1 (MQ=60)
                                                                                                                                         TGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGG             <  SRR19973067.795482/150‑1 (MQ=60)
                                                                                                                                            GTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAG          >  SRR19973067.2438412/1‑150 (MQ=60)
                                                                                                                                              CCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCT        >  SRR19973067.2618939/1‑150 (MQ=60)
                                                                                                                                              CCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCT        <  SRR19973067.1466010/150‑1 (MQ=60)
                                                                                                                                              CCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCT        >  SRR19973067.825787/1‑150 (MQ=60)
                                                                                                                                               CATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGACTCATCGGCAGCTG       >  SRR19973067.2024040/1‑150 (MQ=60)
                                                                                                                                               CATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTG       >  SRR19973067.2680681/1‑150 (MQ=60)
                                                                                                                                                    TAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTC  >  SRR19973067.3539891/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
CCAGCTTGCCTGACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCTGCATTC  >  CP009273/1799981‑1800278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑