Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F434 I0 R1	1130	215.9	7031662	97.1%	6827743	147.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,800,129	C→T	100%	W71* (TGG→TAG)	ydiY ←	acid‑inducible putative outer membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,800,129	0	C	T	97.5%	657.0 / 5.4	205	W71* (TGG→TAG)	ydiY	acid‑inducible putative outer membrane protein
Reads supporting (aligned to +/- strand): ref base C (3/2); new base T (87/112); total (91/114)
Fisher's exact test for biased strand distribution p-value = 6.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 205 total aligned reads displayed.

CTTGCCTGACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAG  >  CP009273/1799985‑1800270

cTTGCCTGACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAgg                                                                                                                                            >  1:656605/1‑148 (MQ=255)

  tGCCTGACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCCGTGGTGTGTCCATACTAGGTc                                                                                                                                          >  1:224311/1‑148 (MQ=255)

  tGCCTGACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTc                                                                                                                                          >  1:926335/1‑148 (MQ=255)

      tGACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCAtgg                                                                                                                                      <  2:1683875/148‑1 (MQ=255)

          cAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATggtggt                                                                                                                                  <  1:2007182/148‑1 (MQ=255)

            aataaataGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGgtgt                                                                                                                                >  2:3125253/1‑148 (MQ=255)

             ataaataGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTc                                                                                                                               >  2:3229426/1‑148 (MQ=255)

                aataGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGc                                                                                                                            >  2:1679403/1‑148 (MQ=255)

                aataGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGc                                                                                                                            >  2:2437346/1‑148 (MQ=255)

                 atcgTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGcc                                                                                                                           <  2:715603/145‑1 (MQ=255)

                 ataGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGcc                                                                                                                           >  1:835362/1‑148 (MQ=255)

                   aGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGt                                                                                                                         >  1:612794/1‑148 (MQ=255)

                    gTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTa                                                                                                                        <  1:1997386/148‑1 (MQ=255)

                     tCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTaa                                                                                                                       >  1:1640860/1‑148 (MQ=255)

                         aaTCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTga                                                                                                                   <  1:1827354/148‑1 (MQ=255)

                         aaTCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTga                                                                                                                   <  1:1827418/148‑1 (MQ=255)

                          aTCAGTTAAATTAACACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTgag                                                                                                                  <  2:2030110/148‑1 (MQ=255)

                          aTCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTgag                                                                                                                  >  1:735443/1‑148 (MQ=255)

                           tCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTgagg                                                                                                                 >  1:2706137/1‑148 (MQ=255)

                            cAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTgagga                                                                                                                >  2:3407742/1‑148 (MQ=255)

                              gTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGc                                                                                                              <  2:1579358/148‑1 (MQ=255)

                              gTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGc                                                                                                              <  1:820965/148‑1 (MQ=255)

                                 aaattaaaACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCttt                                                                                                           >  1:1266477/1‑148 (MQ=255)

                                  aattaaaACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCtttt                                                                                                          >  2:1907577/1‑148 (MQ=255)

                                   attaaaACGGCTACGCCCGCCCGCCGAAAATTTTTCTGAAGAACCCTCATCATAAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTc                                                                                                         >  1:1132759/1‑148 (MQ=255)

                                     taaaACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGt                                                                                                       <  1:3335305/148‑1 (MQ=255)

                                     taaaACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGt                                                                                                       <  1:973332/148‑1 (MQ=255)

                                      aaaaCGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTa                                                                                                      <  2:408809/148‑1 (MQ=255)

                                       aaaCGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTAt                                                                                                     <  2:2975311/148‑1 (MQ=255)

                                        aaCGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTAtt                                                                                                    >  2:2075709/1‑148 (MQ=255)

                                         aCGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTg                                                                                                   >  2:1583901/1‑148 (MQ=255)

                                            gCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCt                                                                                                <  1:2798836/148‑1 (MQ=255)

                                             cTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTg                                                                                               <  1:110481/148‑1 (MQ=255)

                                             cTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTg                                                                                               >  1:3504349/1‑148 (MQ=255)

                                              tACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGa                                                                                              >  1:1357611/1‑148 (MQ=255)

                                                       ccgccgCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAg                                                                                     <  1:2617241/148‑1 (MQ=255)

                                                        cgccgcATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGa                                                                                    <  1:1967963/148‑1 (MQ=255)

                                                        cgccgcATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGa                                                                                    <  1:53178/148‑1 (MQ=255)

                                                         gccgcATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTNGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGat                                                                                   <  1:1779858/148‑1 (MQ=255)

                                                         gccgcATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGat                                                                                   <  1:2275420/148‑1 (MQ=255)

                                                         gccgcATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGat                                                                                   <  1:694026/148‑1 (MQ=255)

                                                           cgcATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGatat                                                                                 <  1:40607/148‑1 (MQ=255)

                                                           cgcATATTTTTCTGAAGAACGATCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGatat                                                                                 <  2:3469560/148‑1 (MQ=255)

                                                             cATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCCAGATATcc                                                                               >  1:20123/1‑148 (MQ=255)

                                                             cATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATcc                                                                               >  1:2231630/1‑148 (MQ=255)

                                                              atatTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCt                                                                              <  2:2323166/148‑1 (MQ=255)

                                                              atatTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCt                                                                              <  2:25410/148‑1 (MQ=255)

                                                              atatTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCt                                                                              <  2:558614/148‑1 (MQ=255)

                                                               tatTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTg                                                                             >  1:2942622/1‑148 (MQ=255)

                                                                  ttttCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGt                                                                          <  2:1540942/148‑1 (MQ=255)

                                                                  ttttCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGt                                                                          <  2:1125973/148‑1 (MQ=255)

                                                                   tttCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGtt                                                                         <  1:2082179/148‑1 (MQ=255)

                                                                    ttCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTc                                                                        <  1:1807753/148‑1 (MQ=255)

                                                                    ttCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTc                                                                        <  1:27678/148‑1 (MQ=255)

                                                                      cTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAg                                                                      <  2:247381/148‑1 (MQ=255)

                                                                      cTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAg                                                                      <  1:1009847/148‑1 (MQ=255)

                                                                        gaagaaCGCTCATCAGTAGAAGAAGTATTACTGGCGTTCCCCCCCAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGAt                                                                    <  2:487680/148‑1 (MQ=255)

                                                                            aaCGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTAcc                                                                >  2:1267098/1‑148 (MQ=255)

                                                                            aaCGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTAcc                                                                >  1:401949/1‑148 (MQ=255)

                                                                            aaCGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTAcc                                                                >  1:2169984/1‑148 (MQ=255)

                                                                              cGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCtt                                                              <  1:3400412/148‑1 (MQ=255)

                                                                                   atcatTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGaaa                                                         >  2:1607603/1‑148 (MQ=255)

                                                                                     catTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAgg                                                       >  2:3261923/1‑148 (MQ=255)

                                                                                      atTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGt                                                      >  1:2588688/1‑148 (MQ=255)

                                                                                      atTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGt                                                      <  1:2454943/148‑1 (MQ=255)

                                                                                       ttAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGtt                                                     <  1:273859/148‑1 (MQ=255)

                                                                                       ttAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGtt                                                     >  2:3337426/1‑148 (MQ=255)

                                                                                        tAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGttt                                                    >  2:3185627/1‑148 (MQ=255)

                                                                                        tAGAAGAAGTATTACTGGCGGTCCCCCACAGCGACCAGGCAGTGGGGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTGCGAAAGGttt                                                    >  2:3183930/1‑148 (MQ=255)

                                                                                          gaagaaGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGttttt                                                  >  2:1187757/1‑148 (MQ=255)

                                                                                          gaagaaGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGttttt                                                  <  1:2650098/148‑1 (MQ=255)

                                                                                           aagaagTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGtttttt                                                 <  1:231224/148‑1 (MQ=255)

                                                                                            agaagTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTa                                                <  1:3125253/148‑1 (MQ=255)

                                                                                            agaagTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTa                                                <  2:636450/148‑1 (MQ=255)

                                                                                             gaagTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAg                                               >  1:1903488/1‑148 (MQ=255)

                                                                                             gaagTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAg                                               >  1:2520322/1‑148 (MQ=255)

                                                                                                    tACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGc                                        <  1:3014610/148‑1 (MQ=255)

                                                                                                    tACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGc                                        <  1:1550490/148‑1 (MQ=255)

                                                                                                       tGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAgg                                     <  2:1360407/148‑1 (MQ=255)

                                                                                                            ttCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGatcat                                >  1:2855140/1‑148 (MQ=255)

                                                                                                             tCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAgg                                     <  2:2260076/142‑1 (MQ=255)

                                                                                                              cccccACATCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTTATTGTGCATTATATCCTGCGTTCAGATTACCTTCGTTAGGTTTTTTAGCGCTGGCAGGTTCATcc                              <  2:1980420/148‑1 (MQ=255)

                                                                                                              cccccACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATcc                              >  1:2426034/1‑148 (MQ=255)

                                                                                                                cccACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCAt                            >  2:1288025/1‑148 (MQ=255)

                                                                                                                 ccACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATg                           >  1:952093/1‑148 (MQ=255)

                                                                                                                 ccACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATg                           >  2:285279/1‑148 (MQ=255)

                                                                                                                 ccACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATg                           >  2:2162000/1‑148 (MQ=255)

                                                                                                                   acaGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGAc                         >  1:1585845/1‑148 (MQ=255)

                                                                                                                    caGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACa                        <  1:596844/148‑1 (MQ=255)

                                                                                                                    caGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACa                        <  2:109420/148‑1 (MQ=255)

                                                                                                                     cgCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAg                       <  2:2846171/147‑1 (MQ=255)

                                                                                                                     aGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAg                       <  1:3330767/148‑1 (MQ=255)

                                                                                                                          ccAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTaaaa                  <  2:2894040/148‑1 (MQ=255)

                                                                                                                             ggCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACg               >  2:703716/1‑148 (MQ=255)

                                                                                                                               cAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGa             <  1:2200350/148‑1 (MQ=255)

                                                                                                                                aGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGaa            <  1:1969555/148‑1 (MQ=255)

                                                                                                                                  tggtgTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAatc          <  2:2771959/148‑1 (MQ=255)

                                                                                                                                  tggtgTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAatc          >  2:1969402/1‑148 (MQ=255)

                                                                                                                                     tgtgtCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAatcatc       <  2:2991658/148‑1 (MQ=255)

                                                                                                                                      gtgtCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCg      >  1:1533202/1‑148 (MQ=255)

CTTGCCTGACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAG  >  CP009273/1799985‑1800270

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GCCAGCTTGCCTGACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCT  >  CP009273/1799980‑1800272

GCCAGCTTGCCTGACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACT                                                                                                                                                 <  SRR19973065.497395/150‑1 (MQ=60)

GCCAGCTTGCCCGACCAAATACATAGTCATAATCAGTTAAATTAACACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACT                                                                                                                                                 <  SRR19973065.2274885/150‑1 (MQ=60)

   AGCTTGCCTGACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGG                                                                                                                                              >  SRR19973065.660934/1‑150 (MQ=60)

     CTTGCCTGACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCCGTGGTGTGTCCATACTAGGTC                                                                                                                                            >  SRR19973065.225968/1‑150 (MQ=60)

     CTTGCCTGACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTC                                                                                                                                            >  SRR19973065.932418/1‑150 (MQ=60)

          CTGACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGT                                                                                                                                       >  SRR19973065.2911716/1‑150 (MQ=60)

          CTGACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGT                                                                                                                                       >  SRR19973065.3061765/1‑150 (MQ=60)

               CAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGT                                                                                                                                  >  SRR19973065.1090119/1‑150 (MQ=60)

               CAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGT                                                                                                                                  <  SRR19973065.2020146/150‑1 (MQ=60)

               CAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGT                                                                                                                                  <  SRR19973065.2020430/150‑1 (MQ=60)

                    AAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCC                                                                                                                             >  SRR19973065.840811/1‑150 (MQ=60)

                      ATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGT                                                                                                                           >  SRR19973065.616708/1‑150 (MQ=60)

                        AGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAA                                                                                                                         >  SRR19973065.1651880/1‑150 (MQ=60)

                         GTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAG                                                                                                                        <  SRR19973065.2010323/150‑1 (MQ=60)

                         GTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAG                                                                                                                        <  SRR19973065.2003499/150‑1 (MQ=60)

                             TAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAG                                                                                                                    >  SRR19973065.740359/1‑150 (MQ=60)

                             TAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAG                                                                                                                    >  SRR19973065.2511376/1‑150 (MQ=60)

                             TAATCAGTTAAATAAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAG                                                                                                                    <  SRR19973065.745232/150‑1 (MQ=60)

                              AATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGG                                                                                                                   >  SRR19973065.2722961/1‑150 (MQ=60)

                              AATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGG                                                                                                                   <  SRR19973065.1839394/150‑1 (MQ=60)

                              AATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGG                                                                                                                   <  SRR19973065.1839449/150‑1 (MQ=60)

                              AATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGG                                                                                                                   <  SRR19973065.1839458/150‑1 (MQ=60)

                                 CAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGC                                                                                                                >  SRR19973065.640610/1‑150 (MQ=60)

                                   GTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTT                                                                                                              <  SRR19973065.826357/150‑1 (MQ=60)

                                   GTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTT                                                                                                              <  SRR19973065.826422/150‑1 (MQ=60)

                                    TTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTT                                                                                                             >  SRR19973065.1274825/1‑150 (MQ=60)

                                      AAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTC                                                                                                           <  SRR19973065.2871480/150‑1 (MQ=60)

                                      AAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTC                                                                                                           >  SRR19973065.1140130/1‑150 (MQ=60)

                                      AAATTAAAACGGCTACGCCCGCCCGCCGAAAATTTTTCTGAAGAACCCTCATCATAAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTC                                                                                                           >  SRR19973065.1140109/1‑150 (MQ=60)

                                      AAATTAAAACGGCTACGCCCGCCCGCAGCATATTTTTCTGAAGAACGATCATCATTAGAAGTAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTC                                                                                                           <  SRR19973065.2196866/150‑1 (MQ=60)

                                          TAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTAT                                                                                                       <  SRR19973065.3355665/150‑1 (MQ=60)

                                          TAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTAT                                                                                                       <  SRR19973065.981814/150‑1 (MQ=60)

                                          TAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTAT                                                                                                       <  SRR19973065.979793/150‑1 (MQ=60)

                                             AACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGC                                                                                                    >  SRR19973065.1829287/1‑150 (MQ=60)

                                                GGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACAAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTG                                                                                                 >  SRR19973065.3525628/1‑150 (MQ=60)

                                                 GCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGA                                                                                                <  SRR19973065.2816024/150‑1 (MQ=60)

                                                 GCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGA                                                                                                <  SRR19973065.2816176/150‑1 (MQ=60)

                                                 GCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGA                                                                                                >  SRR19973065.1366606/1‑150 (MQ=60)

                                                  CTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGAT                                                                                               <  SRR19973065.111480/150‑1 (MQ=60)

                                                  CTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGAT                                                                                               <  SRR19973065.112078/150‑1 (MQ=60)

                                                   TACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATT                                                                                              <  SRR19973065.2010860/150‑1 (MQ=60)

                                                            CCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGAT                                                                                     <  SRR19973065.2633453/150‑1 (MQ=60)

                                                            CCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGAT                                                                                     <  SRR19973065.3346639/150‑1 (MQ=60)

                                                             CGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATA                                                                                    <  SRR19973065.1980800/150‑1 (MQ=60)

                                                             CGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATA                                                                                    <  SRR19973065.1980829/150‑1 (MQ=60)

                                                             CGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATA                                                                                    <  SRR19973065.53763/150‑1 (MQ=60)

                                                             CGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATA                                                                                    <  SRR19973065.1720191/150‑1 (MQ=60)

                                                              GCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTNGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATAT                                                                                   <  SRR19973065.1791481/150‑1 (MQ=60)

                                                              GCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTNGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATAT                                                                                   <  SRR19973065.1791478/150‑1 (MQ=60)

                                                              GCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATAT                                                                                   <  SRR19973065.698764/150‑1 (MQ=60)

                                                              GCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATAT                                                                                   <  SRR19973065.698649/150‑1 (MQ=60)

                                                              GCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATAT                                                                                   <  SRR19973065.2762191/150‑1 (MQ=60)

                                                              GCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATAT                                                                                   <  SRR19973065.2289778/150‑1 (MQ=60)

                                                              GCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATAT                                                                                   <  SRR19973065.2289786/150‑1 (MQ=60)

                                                               CCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATC                                                                                  <  SRR19973065.697408/150‑1 (MQ=60)

                                                                CGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCCAGATATCC                                                                                 >  SRR19973065.20404/1‑150 (MQ=60)

                                                                CGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCC                                                                                 <  SRR19973065.2341514/150‑1 (MQ=60)

                                                                CGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCC                                                                                 <  SRR19973065.39181/150‑1 (MQ=60)

                                                                CGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCC                                                                                 <  SRR19973065.41101/150‑1 (MQ=60)

                                                                CGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCC                                                                                 <  SRR19973065.41103/150‑1 (MQ=60)

                                                                CGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCC                                                                                 >  SRR19973065.2245843/1‑150 (MQ=60)

                                                                CGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCC                                                                                 >  SRR19973065.2245829/1‑150 (MQ=60)

                                                                  CATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTG                                                                               >  SRR19973065.2960492/1‑150 (MQ=60)

                                                                       TTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTC                                                                          >  SRR19973065.953699/1‑150 (MQ=60)

                                                                        TTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCA                                                                         <  SRR19973065.2095522/150‑1 (MQ=60)

                                                                         TTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAG                                                                        <  SRR19973065.1819647/150‑1 (MQ=60)

                                                                         TTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAG                                                                        <  SRR19973065.28039/150‑1 (MQ=60)

                                                                           CTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGAT                                                                      <  SRR19973065.1016543/150‑1 (MQ=60)

                                                                            TGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATT                                                                     >  SRR19973065.613571/1‑150 (MQ=60)

                                                                             GAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTA                                                                    <  SRR19973065.578566/150‑1 (MQ=60)

                                                                               AGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACC                                                                  >  SRR19973065.404768/1‑150 (MQ=60)

                                                                               AGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACC                                                                  <  SRR19973065.2160477/150‑1 (MQ=60)

                                                                               AGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACC                                                                  >  SRR19973065.2183916/1‑150 (MQ=60)

                                                                                  ACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTC                                                               <  SRR19973065.2989478/150‑1 (MQ=60)

                                                                                   CGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCG                                                              <  SRR19973065.3420635/150‑1 (MQ=60)

                                                                                   CGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCG                                                              <  SRR19973065.3421196/150‑1 (MQ=60)

                                                                                   CGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCG                                                              <  SRR19973065.3421202/150‑1 (MQ=60)

                                                                                     CTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAA                                                            <  SRR19973065.1953990/150‑1 (MQ=60)

                                                                                         TCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGT                                                        >  SRR19973065.2604803/1‑150 (MQ=60)

                                                                                           ATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTT                                                      >  SRR19973065.2172704/1‑150 (MQ=60)

                                                                                           ATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTT                                                      <  SRR19973065.2470413/150‑1 (MQ=60)

                                                                                            TTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGTGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTT                                                     <  SRR19973065.2060127/150‑1 (MQ=60)

                                                                                            TTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTT                                                     <  SRR19973065.275700/150‑1 (MQ=60)

                                                                                             TAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACAAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTT                                                    >  SRR19973065.1863159/1‑150 (MQ=60)

                                                                                               GAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTA                                                  <  SRR19973065.2666439/150‑1 (MQ=60)

                                                                                               GAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTA                                                  <  SRR19973065.2669287/150‑1 (MQ=60)

                                                                                                AAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAG                                                 <  SRR19973065.232906/150‑1 (MQ=60)

                                                                                                AAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAG                                                 >  SRR19973065.1915996/1‑150 (MQ=60)

                                                                                                AAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAG                                                 >  SRR19973065.2536146/1‑150 (MQ=60)

                                                                                                 AGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGC                                                <  SRR19973065.3144613/150‑1 (MQ=60)

                                                                                                 AGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGC                                                <  SRR19973065.3144284/150‑1 (MQ=60)

                                                                                                         TACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAG                                        <  SRR19973065.1275554/150‑1 (MQ=60)

                                                                                                         TACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAG                                        <  SRR19973065.1560752/150‑1 (MQ=60)

                                                                                                         TACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAG                                        <  SRR19973065.3033072/150‑1 (MQ=60)

                                                                                                             GGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATC                                    <  SRR19973065.894334/150‑1 (MQ=60)

                                                                                                               CGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCAT                                  >  SRR19973065.2872688/1‑150 (MQ=60)

                                                                                                                GTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATagat                                 >  SRR19973065.2274369/1‑146 (MQ=60)

                                                                                                                 TTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCC                                >  SRR19973065.2441342/1‑150 (MQ=60)

                                                                                                                 TTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCC                                >  SRR19973065.2441475/1‑150 (MQ=60)

                                                                                                                   CCCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCAT                              <  SRR19973065.2649148/150‑1 (MQ=60)

                                                                                                                    CCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATG                             >  SRR19973065.958421/1‑150 (MQ=60)

                                                                                                                    CCCCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATG                             >  SRR19973065.958387/1‑150 (MQ=60)

                                                                                                                      CCACAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGAC                           >  SRR19973065.1596420/1‑150 (MQ=60)

                                                                                                                         CAGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGT                        <  SRR19973065.600551/150‑1 (MQ=60)

                                                                                                                          AGCGACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTA                       <  SRR19973065.3351100/150‑1 (MQ=60)

                                                                                                                             GACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAA                    >  SRR19973065.2602826/1‑150 (MQ=60)

                                                                                                                              ACCAGGCAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAA                   >  SRR19973065.1622709/1‑150 (MQ=60)

                                                                                                                                    CAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAAT             <  SRR19973065.1594463/150‑1 (MQ=60)

                                                                                                                                    CAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAAT             <  SRR19973065.2214436/150‑1 (MQ=60)

                                                                                                                                    CAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAAT             <  SRR19973065.2214416/150‑1 (MQ=60)

                                                                                                                                    CAGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCACCCATGACAGTAAAAACGGAAT             <  SRR19973065.2214442/150‑1 (MQ=60)

                                                                                                                                     AGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATC            <  SRR19973065.1982399/150‑1 (MQ=60)

                                                                                                                                     AGTGGTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATC            >  SRR19973065.140243/1‑150 (MQ=60)

                                                                                                                                         GTGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCG        <  SRR19973065.424395/150‑1 (MQ=60)

                                                                                                                                         GTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCG        >  SRR19973065.1543311/1‑150 (MQ=60)

                                                                                                                                         GTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCG        >  SRR19973065.1543343/1‑150 (MQ=60)

                                                                                                                                          TGTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGG       <  SRR19973065.2726628/150‑1 (MQ=60)

                                                                                                                                           GTGTCCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGC      >  SRR19973065.3353868/1‑150 (MQ=60)

                                                                                                                                               CCATACTAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCT  <  SRR19973065.2621467/150‑1 (MQ=60)

GCCAGCTTGCCTGACCAAATAAATAGTCATAATCAGTTAAATTAAAACGGCTACGCCCGCCCGCCGCATATTTTTCTGAAGAACGCTCATCATTAGAAGAAGTATTACTGGCGTTCCCCCACAGCGACCAGGCAGTGGTGTGTCCATACCAGGTCATGGTGGTGTCAGCCGTAAGTGAGGAGCTTTTCGTATTGCCTGATTGTGCAAGATATCCTGCGTTCAGATTACCTTCGAAAGGTTTTTTAGCGCTGGCAGGATCATCCATGACAGTAAAAACGGAATCATCGGCAGCT  >  CP009273/1799980‑1800272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`