Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,872,054 A→T 100% E28V (GAG→GTG)  yeaP → diguanylate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,872,0540AT99.5% 639.5 / ‑4.6 193E28V (GAG→GTG) yeaPdiguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base T (88/104);  total (89/104)
Fisher's exact test for biased strand distribution p-value = 4.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 196 total aligned reads displayed.

GTTTTATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTACTCG  >  CP009273/1871909‑1872196
                                                                                                                                                 |                                                                                                                                              
gTTTTATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGgtga                                                                                                                                              <  1:551260/148‑1 (MQ=255)
 ttttATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGACTGGTCCGACAGCTTCTGGTGAt                                                                                                                                             >  2:1486148/1‑148 (MQ=255)
   ttATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGc                                                                                                                                           >  1:1648960/1‑148 (MQ=255)
    tATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCt                                                                                                                                          <  2:658274/148‑1 (MQ=255)
        aTGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGac                                                                                                                                      >  1:2770486/1‑147 (MQ=255)
         tGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGaaa                                                                                                                                     <  2:576339/148‑1 (MQ=255)
             tGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGt                                                                                                                                 <  2:1778860/148‑1 (MQ=255)
             tGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGt                                                                                                                                 >  1:1777471/1‑148 (MQ=255)
               cAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCa                                                                                                                               <  2:3520397/148‑1 (MQ=255)
                 aaGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACt                                                                                                                             >  2:2071377/1‑148 (MQ=255)
                 aaGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACt                                                                                                                             >  2:2071381/1‑148 (MQ=255)
                 aaGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACt                                                                                                                             >  2:2186456/1‑148 (MQ=255)
                  aGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTg                                                                                                                            >  2:1793908/1‑148 (MQ=255)
                    aTTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGat                                                                                                                          >  2:3487630/1‑148 (MQ=255)
                     ttAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAAACCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAAAGGTCACTGata                                                                                                                         >  1:954510/1‑148 (MQ=255)
                         ataatCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGa                                                                                                                     >  1:1793311/1‑148 (MQ=255)
                         ataatCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGa                                                                                                                     >  1:2165109/1‑148 (MQ=255)
                              cAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGACCAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCaa                                                                                                                >  2:3117972/1‑148 (MQ=255)
                              cAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCaa                                                                                                                <  1:135320/148‑1 (MQ=255)
                              cAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCaa                                                                                                                <  1:1932929/148‑1 (MQ=255)
                               aGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAac                                                                                                               <  2:764585/148‑1 (MQ=255)
                                    ttACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAacctacc                                                                                                          <  1:2740610/148‑1 (MQ=255)
                                    ttACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAacctacc                                                                                                          >  1:1019660/1‑148 (MQ=255)
                                       cccGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGa                                                                                                       <  1:2233884/148‑1 (MQ=255)
                                        ccgccgTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGAc                                                                                                      <  1:1602927/148‑1 (MQ=255)
                                           ccgTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCaa                                                                                                   >  2:1099881/1‑148 (MQ=255)
                                              tATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGt                                                                                                >  1:1831004/1‑148 (MQ=255)
                                              tATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGt                                                                                                <  1:2684319/148‑1 (MQ=255)
                                              tATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGt                                                                                                <  1:2687512/148‑1 (MQ=255)
                                               aTCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTg                                                                                               >  1:308038/1‑148 (MQ=255)
                                                 ccTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGa                                                                                             >  1:3258146/1‑148 (MQ=255)
                                                 ccTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGa                                                                                             >  1:3179428/1‑148 (MQ=255)
                                                 ccTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGa                                                                                             >  1:1409524/1‑148 (MQ=255)
                                                 ccTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCACGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGa                                                                                             <  1:1882622/148‑1 (MQ=255)
                                                  cTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGAt                                                                                            <  1:1272300/148‑1 (MQ=255)
                                                   tGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATg                                                                                           >  1:1169944/1‑148 (MQ=255)
                                                     gAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATAAACCTACCTGACCAAAGTGGATGTc                                                                                         >  1:2452115/1‑148 (MQ=255)
                                                        ttgttCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGaa                                                                                      >  1:633572/1‑148 (MQ=255)
                                                        ttgttCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGaa                                                                                      >  2:122453/1‑148 (MQ=255)
                                                        ttgttCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGaa                                                                                      <  1:1915490/148‑1 (MQ=255)
                                                           ttCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAgcg                                                                                   <  1:2798056/148‑1 (MQ=255)
                                                            tCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAgcgc                                                                                  >  2:3156579/1‑148 (MQ=255)
                                                              cGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAgcgcgc                                                                                <  2:1335387/148‑1 (MQ=255)
                                                              cGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAgcgcgc                                                                                <  1:3157742/148‑1 (MQ=255)
                                                              cGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAgcgcgc                                                                                <  1:1593905/148‑1 (MQ=255)
                                                              cGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAgcgcgc                                                                                <  2:1992531/148‑1 (MQ=255)
                                                              cGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAgcgcgc                                                                                <  2:2648846/148‑1 (MQ=255)
                                                                 gtCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTg                                                                             >  1:711797/1‑148 (MQ=255)
                                                                 gtCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTg                                                                             >  1:711804/1‑148 (MQ=255)
                                                                 gtCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTg                                                                             <  1:1523888/148‑1 (MQ=255)
                                                                   cagatcagatTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTgca                                                                           >  1:1973970/1‑148 (MQ=255)
                                                                    agatcagatTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTgcag                                                                          <  1:936252/148‑1 (MQ=255)
                                                                          gatTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCAtatt                                                                    <  2:3246882/148‑1 (MQ=255)
                                                                          gatTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCAtatt                                                                    <  1:1915936/148‑1 (MQ=255)
                                                                          gatTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCAtatt                                                                    <  1:1488965/148‑1 (MQ=255)
                                                                                  cccGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGc                                                            >  2:3277395/1‑148 (MQ=255)
                                                                                   ccGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGcc                                                           >  2:2643826/1‑148 (MQ=255)
                                                                                       gTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTa                                                       <  1:586149/148‑1 (MQ=255)
                                                                                        tctcGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTaa                                                      >  2:1569881/1‑148 (MQ=255)
                                                                                        tctcGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTaa                                                      <  1:2419606/148‑1 (MQ=255)
                                                                                         ctcGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAAc                                                     <  2:3064627/148‑1 (MQ=255)
                                                                                            gCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGc                                                  <  1:444585/148‑1 (MQ=255)
                                                                                              aaTCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCa                                                >  1:2078095/1‑148 (MQ=255)
                                                                                               aTCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAg                                               >  1:1200022/1‑148 (MQ=255)
                                                                                                tCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGa                                              >  1:105986/1‑148 (MQ=255)
                                                                                                tCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGa                                              <  1:2944982/148‑1 (MQ=255)
                                                                                                  ccTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGaaa                                            <  1:2898114/148‑1 (MQ=255)
                                                                                                   cTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGaaaa                                           <  2:2518149/148‑1 (MQ=255)
                                                                                                   cTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGaaaa                                           >  2:1798203/1‑148 (MQ=255)
                                                                                                    ttGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAAt                                          >  2:1408085/1‑148 (MQ=255)
                                                                                                     tGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATg                                         <  2:3402930/148‑1 (MQ=255)
                                                                                                       ccAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTa                                       <  1:566305/148‑1 (MQ=255)
                                                                                                       ccAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTa                                       >  1:3507621/1‑148 (MQ=255)
                                                                                                        cAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTAc                                      >  2:42477/1‑148 (MQ=255)
                                                                                                           aGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTGTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATc                                   <  2:2817223/148‑1 (MQ=255)
                                                                                                           aGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATc                                   >  2:2744588/1‑148 (MQ=255)
                                                                                                           aGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATc                                   <  2:1979818/148‑1 (MQ=255)
                                                                                                                    aCTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAAt                          >  1:1813081/1‑148 (MQ=255)
                                                                                                                     cTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAAtt                         <  2:1820106/148‑1 (MQ=255)
                                                                                                                       ggAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAAtttt                       <  2:903946/148‑1 (MQ=255)
                                                                                                                        gAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTa                      <  1:1086413/148‑1 (MQ=255)
                                                                                                                        gAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTa                      <  2:2584305/148‑1 (MQ=255)
                                                                                                                        gAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTa                      <  1:1400395/148‑1 (MQ=255)
                                                                                                                          aaGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTAcc                    >  2:290409/1‑148 (MQ=255)
                                                                                                                                ggTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCg              <  1:2608621/148‑1 (MQ=255)
                                                                                                                                ggTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCg              <  2:1500544/148‑1 (MQ=255)
                                                                                                                                  tCCGACAGCTTCTGGTGATGCTGTAAATGGTCACTGATATTGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTTACAGCCAGAAAATGTACATCCCTGAGAATTTTACCGTCTCGTg            <  2:1758137/148‑1 (MQ=255)
                                                                                                                                  tCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTg            <  2:1931945/148‑1 (MQ=255)
                                                                                                                                    cGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTggg          <  2:94793/148‑1 (MQ=255)
                                                                                                                                    cGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTggg          <  2:1892874/148‑1 (MQ=255)
                                                                                                                                    cGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTggg          >  1:2649970/1‑148 (MQ=255)
                                                                                                                                     gACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGa         <  2:179179/148‑1 (MQ=255)
                                                                                                                                      aCAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGAt        >  1:2000299/1‑148 (MQ=255)
                                                                                                                                      aCAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGAt        <  2:1349403/148‑1 (MQ=255)
                                                                                                                                      aCAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGAt        <  2:1019660/148‑1 (MQ=255)
                                                                                                                                       cAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGAtt       >  2:3333252/1‑148 (MQ=255)
                                                                                                                                       cAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGAtt       >  2:1860266/1‑148 (MQ=255)
                                                                                                                                       cAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGAtt       >  2:1361697/1‑148 (MQ=255)
                                                                                                                                       cAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGAtt       <  1:1001365/148‑1 (MQ=255)
                                                                                                                                          cTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTACt    <  1:2474674/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                              
GTTTTATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTACTCG  >  CP009273/1871909‑1872196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGATGTTTTATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTACTCGTTATG  >  CP009273/1871905‑1872201
                                                                                                                                                     |                                                                                                                                                   
TGATGTTTTATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGT                                                                                                                                                     >  SRR19973067.2934939/1‑150 (MQ=60)
    GTTTTATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATG                                                                                                                                                 <  SRR19973067.554891/150‑1 (MQ=60)
    GTTTTATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATG                                                                                                                                                 <  SRR19973067.554893/150‑1 (MQ=60)
     TTTTATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGC                                                                                                                                                >  SRR19973067.1660318/1‑150 (MQ=60)
          TCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAC                                                                                                                                           >  SRR19973067.2788383/1‑150 (MQ=60)
          TCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAA                                                                                                                                           >  SRR19973067.2788401/1‑150 (MQ=60)
            ATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAAT                                                                                                                                         >  SRR19973067.843307/1‑150 (MQ=60)
             TGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATG                                                                                                                                        <  SRR19973067.2450382/150‑1 (MQ=60)
               CCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGT                                                                                                                                      >  SRR19973067.1789505/1‑150 (MQ=60)
               CCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGT                                                                                                                                      <  SRR19973067.3002780/150‑1 (MQ=60)
                CTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGTCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTC                                                                                                                                     >  SRR19973067.203381/1‑150 (MQ=60)
                CTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTC                                                                                                                                     >  SRR19973067.1124268/1‑150 (MQ=60)
                   CAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACT                                                                                                                                  >  SRR19973067.2702100/1‑150 (MQ=60)
                    AAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTG                                                                                                                                 >  SRR19973067.95783/1‑150 (MQ=60)
                    AAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTG                                                                                                                                 >  SRR19973067.1971270/1‑150 (MQ=60)
                    AAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTG                                                                                                                                 >  SRR19973067.1905795/1‑150 (MQ=60)
                       GATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAAACCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAAAGGTCACTGATA                                                                                                                              >  SRR19973067.961047/1‑150 (MQ=60)
                         TTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATG                                                                                                                            <  SRR19973067.511271/150‑1 (MQ=60)
                         TTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATG                                                                                                                            >  SRR19973067.1835907/1‑150 (MQ=60)
                         TTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATG                                                                                                                            <  SRR19973067.612579/150‑1 (MQ=60)
                           AAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGA                                                                                                                          >  SRR19973067.2179584/1‑150 (MQ=60)
                           AAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGA                                                                                                                          >  SRR19973067.1805387/1‑150 (MQ=60)
                           AAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGA                                                                                                                          >  SRR19973067.1805378/1‑150 (MQ=60)
                             ATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAAT                                                                                                                        <  SRR19973067.2222803/150‑1 (MQ=60)
                               AATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCA                                                                                                                      >  SRR19973067.1482511/1‑150 (MQ=60)
                               AATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCA                                                                                                                      >  SRR19973067.574135/1‑150 (MQ=60)
                                 TCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAAC                                                                                                                    <  SRR19973067.1879854/150‑1 (MQ=60)
                                  CAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACC                                                                                                                   <  SRR19973067.1945484/150‑1 (MQ=60)
                                  CAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACC                                                                                                                   <  SRR19973067.136527/150‑1 (MQ=60)
                                  CAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACC                                                                                                                   <  SRR19973067.1946114/150‑1 (MQ=60)
                                      ATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACC                                                                                                               >  SRR19973067.1026661/1‑150 (MQ=60)
                                        TTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTG                                                                                                             <  SRR19973067.2758317/150‑1 (MQ=60)
                                        TTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTG                                                                                                             <  SRR19973067.2758303/150‑1 (MQ=60)
                                           CCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACC                                                                                                          <  SRR19973067.2248746/150‑1 (MQ=60)
                                           CCCGCAGTATCCTGGAGTTGTTCAGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGATATGGTCACTGATATGGAATCATCCTACCTGACC                                                                                                          <  SRR19973067.446939/150‑1 (MQ=60)
                                            CCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCA                                                                                                         <  SRR19973067.1613942/150‑1 (MQ=60)
                                            CCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCA                                                                                                         <  SRR19973067.1613931/150‑1 (MQ=60)
                                                CGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGT                                                                                                     >  SRR19973067.1843453/1‑150 (MQ=60)
                                                 GTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTG                                                                                                    >  SRR19973067.1056379/1‑150 (MQ=60)
                                                 GTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTG                                                                                                    >  SRR19973067.310043/1‑150 (MQ=60)
                                                  TATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGG                                                                                                   <  SRR19973067.2704758/150‑1 (MQ=60)
                                                  TATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGG                                                                                                   <  SRR19973067.2701529/150‑1 (MQ=60)
                                                   ATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGA                                                                                                  >  SRR19973067.3278506/1‑150 (MQ=60)
                                                   ATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGA                                                                                                  >  SRR19973067.1419069/1‑150 (MQ=60)
                                                   ATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGA                                                                                                  >  SRR19973067.3278490/1‑150 (MQ=60)
                                                   ATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGA                                                                                                  >  SRR19973067.3199543/1‑150 (MQ=60)
                                                    TCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGANAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGAT                                                                                                 <  SRR19973067.1813504/150‑1 (MQ=60)
                                                     CCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATG                                                                                                >  SRR19973067.1177703/1‑150 (MQ=60)
                                                     CCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCACGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATG                                                                                                <  SRR19973067.1895472/150‑1 (MQ=60)
                                                      CTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGT                                                                                               <  SRR19973067.1280955/150‑1 (MQ=60)
                                                       TGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATAAACCTACCTGACCAAAGTGGATGTC                                                                                              >  SRR19973067.2468233/1‑150 (MQ=60)
                                                          AGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAA                                                                                           >  SRR19973067.637819/1‑150 (MQ=60)
                                                            TTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGC                                                                                         <  SRR19973067.1928564/150‑1 (MQ=60)
                                                            TTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGC                                                                                         >  SRR19973067.3153646/1‑150 (MQ=60)
                                                               TTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCG                                                                                      <  SRR19973067.2816104/150‑1 (MQ=60)
                                                                  CGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCT                                                                                   <  SRR19973067.3177773/150‑1 (MQ=60)
                                                                  CGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCT                                                                                   <  SRR19973067.3177754/150‑1 (MQ=60)
                                                                  CGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCT                                                                                   <  SRR19973067.1604831/150‑1 (MQ=60)
                                                                  CGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCT                                                                                   <  SRR19973067.1604755/150‑1 (MQ=60)
                                                                   GTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTG                                                                                  >  SRR19973067.716726/1‑150 (MQ=60)
                                                                   GTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTG                                                                                  >  SRR19973067.716733/1‑150 (MQ=60)
                                                                     GTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCA                                                                                <  SRR19973067.1390242/150‑1 (MQ=60)
                                                                     GTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCA                                                                                >  SRR19973067.1987348/1‑150 (MQ=60)
                                                                     GTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCA                                                                                <  SRR19973067.1534124/150‑1 (MQ=60)
                                                                     GTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCA                                                                                >  SRR19973067.178071/1‑150 (MQ=60)
                                                                        agatcagcggagCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCA                                                                             <  SRR19973067.1718178/138‑1 (MQ=60)
                                                                        AGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCA                                                                             <  SRR19973067.942609/150‑1 (MQ=60)
                                                                              GATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTAT                                                                       <  SRR19973067.119149/150‑1 (MQ=60)
                                                                              GATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTAT                                                                       <  SRR19973067.1929012/150‑1 (MQ=60)
                                                                              GATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTAT                                                                       <  SRR19973067.1929001/150‑1 (MQ=60)
                                                                              GATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTAT                                                                       <  SRR19973067.1498821/150‑1 (MQ=60)
                                                                                           GTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAAC                                                          <  SRR19973067.589893/150‑1 (MQ=60)
                                                                                            TCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACA                                                         <  SRR19973067.2435460/150‑1 (MQ=60)
                                                                                             CTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAG                                                        >  SRR19973067.1605643/1‑150 (MQ=60)
                                                                                              TCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGC                                                       <  SRR19973067.3509762/150‑1 (MQ=60)
                                                                                              TCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGC                                                       <  SRR19973067.28595/150‑1 (MQ=60)
                                                                                                GCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCA                                                     >  SRR19973067.2091874/1‑150 (MQ=60)
                                                                                                GCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCA                                                     <  SRR19973067.447671/150‑1 (MQ=60)
                                                                                                 CAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAG                                                    >  SRR19973067.1207942/1‑150 (MQ=60)
                                                                                                 CAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAG                                                    >  SRR19973067.1207871/1‑150 (MQ=60)
                                                                                                  AATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGA                                                   >  SRR19973067.107033/1‑150 (MQ=60)
                                                                                                    TCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAA                                                 <  SRR19973067.2963635/150‑1 (MQ=60)
                                                                                                      CCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAAT                                               <  SRR19973067.2916607/150‑1 (MQ=60)
                                                                                                         TGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTA                                            >  SRR19973067.3529835/1‑150 (MQ=60)
                                                                                                          GCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTAC                                           <  SRR19973067.660585/150‑1 (MQ=60)
                                                                                                           CCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACA                                          <  SRR19973067.569993/150‑1 (MQ=60)
                                                                                                               AGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCC                                      <  SRR19973067.2460910/150‑1 (MQ=60)
                                                                                                                GAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCG                                     <  SRR19973067.262934/150‑1 (MQ=60)
                                                                                                                  ACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGA                                   <  SRR19973067.2769091/150‑1 (MQ=60)
                                                                                                                   CAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAATCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAG                                  <  SRR19973067.1507697/150‑1 (MQ=60)
                                                                                                                    AGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGA                                 >  SRR19973067.2927338/1‑150 (MQ=60)
                                                                                                                      TCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAAT                               >  SRR19973067.1825347/1‑150 (MQ=60)
                                                                                                                            GAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACC                         <  SRR19973067.1092824/150‑1 (MQ=60)
                                                                                                                            GAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACC                         <  SRR19973067.1410270/150‑1 (MQ=60)
                                                                                                                            GAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACC                         <  SRR19973067.1409896/150‑1 (MQ=60)
                                                                                                                            GAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACC                         <  SRR19973067.1093842/150‑1 (MQ=60)
                                                                                                                             AAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCG                        >  SRR19973067.1352056/1‑150 (MQ=60)
                                                                                                                                    GGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGNG                 <  SRR19973067.2625565/150‑1 (MQ=60)
                                                                                                                                      TCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGG               >  SRR19973067.2667060/1‑150 (MQ=60)
                                                                                                                                       CCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGA              <  SRR19973067.2403119/150‑1 (MQ=60)
                                                                                                                                        CGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGAT             >  SRR19973067.2013792/1‑150 (MQ=60)
                                                                                                                                          ACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGTCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTA           <  SRR19973067.2001057/150‑1 (MQ=60)
                                                                                                                                           CAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTAC          <  SRR19973067.1008248/150‑1 (MQ=60)
                                                                                                                                              CTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTACTCG       <  SRR19973067.2490839/150‑1 (MQ=60)
                                                                                                                                              CTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTACTCG       <  SRR19973067.2490946/150‑1 (MQ=60)
                                                                                                                                              CTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTACTCG       <  SRR19973067.2490976/150‑1 (MQ=60)
                                                                                                                                              CTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTACTCG       >  SRR19973067.2668632/1‑150 (MQ=60)
                                                                                                                                                   GGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTACTCGTTATG  >  SRR19973067.214885/1‑150 (MQ=60)
                                                                                                                                                   GGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTACTCGTTATG  >  SRR19973067.215208/1‑150 (MQ=60)
                                                                                                                                                   GGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTACTCGTTATG  >  SRR19973067.214835/1‑150 (MQ=60)
                                                                                                                                                   GGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTACTCGTTATG  <  SRR19973067.2518514/150‑1 (MQ=60)
                                                                                                                                                   GGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTACTCGTTATG  >  SRR19973067.1524775/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                   
TGATGTTTTATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTACTCGTTATG  >  CP009273/1871905‑1872201

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑