Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,872,054 A→T 100% E28V (GAG→GTG)  yeaP → diguanylate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,872,0540AT96.6% 733.8 / 11.7 232E28V (GAG→GTG) yeaPdiguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base A (3/5);  new base T (110/114);  total (113/119)
Fisher's exact test for biased strand distribution p-value = 7.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 235 total aligned reads displayed.

GTTTTATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTACTCGTT  >  CP009273/1871909‑1872198
                                                                                                                                                 |                                                                                                                                                
  tttATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATg                                                                                                                                              >  1:339025/1‑148 (MQ=255)
      tCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTgg                                                                                                                                          >  2:176754/1‑148 (MQ=255)
       cATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGa                                                                                                                                         >  1:859074/1‑148 (MQ=255)
       cATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGa                                                                                                                                         >  1:543110/1‑148 (MQ=255)
        aTGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGaa                                                                                                                                        <  1:237703/148‑1 (MQ=255)
          gCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAAt                                                                                                                                      <  2:2045228/148‑1 (MQ=255)
           ccTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATg                                                                                                                                     >  2:3445878/1‑148 (MQ=255)
           ccTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATg                                                                                                                                     <  2:2119411/148‑1 (MQ=255)
             tGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGt                                                                                                                                   <  2:3533347/148‑1 (MQ=255)
               cAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCa                                                                                                                                 <  1:3543216/148‑1 (MQ=255)
               cAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCa                                                                                                                                 <  1:3324608/148‑1 (MQ=255)
                aaaGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCAc                                                                                                                                >  1:2930425/1‑148 (MQ=255)
                 aaGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACt                                                                                                                               >  1:2216150/1‑148 (MQ=255)
                  aGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTg                                                                                                                              >  1:3430323/1‑148 (MQ=255)
                  aGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTg                                                                                                                              >  2:1729888/1‑148 (MQ=255)
                  aGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTg                                                                                                                              <  1:2501205/148‑1 (MQ=255)
                   gATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGa                                                                                                                             <  2:335619/148‑1 (MQ=255)
                    aTTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGat                                                                                                                            <  1:2593271/148‑1 (MQ=255)
                         ataatCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGa                                                                                                                       >  1:3222651/1‑148 (MQ=255)
                          taatCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGaa                                                                                                                      <  2:683653/148‑1 (MQ=255)
                           aatCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAAt                                                                                                                     <  1:1250551/148‑1 (MQ=255)
                            atCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATc                                                                                                                    >  1:916875/1‑148 (MQ=255)
                             tCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCa                                                                                                                   >  2:319176/1‑148 (MQ=255)
                              cAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCaa                                                                                                                  <  1:1525034/148‑1 (MQ=255)
                               aGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAac                                                                                                                 <  2:3147201/148‑1 (MQ=255)
                               aGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAac                                                                                                                 <  1:2888282/148‑1 (MQ=255)
                                 cATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAacct                                                                                                               >  1:2466556/1‑148 (MQ=255)
                                 cATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAacct                                                                                                               >  1:2679542/1‑148 (MQ=255)
                                   tttACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAacctac                                                                                                             >  2:3339990/1‑148 (MQ=255)
                                    ttACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAacctacc                                                                                                            >  2:516130/1‑148 (MQ=255)
                                      aCCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAGCCTACCTg                                                                                                          <  2:1288073/148‑1 (MQ=255)
                                      aCCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTg                                                                                                          <  1:2525003/148‑1 (MQ=255)
                                            cgTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCaaa                                                                                                    >  2:1118927/1‑148 (MQ=255)
                                             gTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAg                                                                                                   <  2:482188/148‑1 (MQ=255)
                                              tATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGt                                                                                                  <  1:1289459/148‑1 (MQ=255)
                                               aTCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTg                                                                                                 >  2:2376556/1‑148 (MQ=255)
                                                tCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTgg                                                                                                <  2:3262059/148‑1 (MQ=255)
                                                tCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTgg                                                                                                >  1:1674811/1‑148 (MQ=255)
                                                 ccTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGa                                                                                               >  1:3055485/1‑148 (MQ=255)
                                                 ccTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGa                                                                                               >  2:1366858/1‑148 (MQ=255)
                                                 ccTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGa                                                                                               >  1:435056/1‑148 (MQ=255)
                                                    ggAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGt                                                                                            >  2:911585/1‑148 (MQ=255)
                                                     gAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTc                                                                                           >  2:1594765/1‑148 (MQ=255)
                                                      aGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCg                                                                                          >  1:72785/1‑148 (MQ=255)
                                                        ttgttCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGaa                                                                                        >  1:968876/1‑148 (MQ=255)
                                                        ttgttCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGaa                                                                                        >  1:941752/1‑148 (MQ=255)
                                                         tgttCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAg                                                                                       <  1:1308763/148‑1 (MQ=255)
                                                         tgttCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAg                                                                                       <  1:596550/148‑1 (MQ=255)
                                                                tgtCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCt                                                                                >  1:1172579/1‑148 (MQ=255)
                                                                      atcagatTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTgcagca                                                                          <  2:933093/148‑1 (MQ=255)
                                                                        cagatTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCata                                                                        <  2:1155175/148‑1 (MQ=255)
                                                                          gatTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCAtatt                                                                      <  2:273301/148‑1 (MQ=255)
                                                                           atTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCAtatta                                                                     >  2:1248316/1‑148 (MQ=255)
                                                                           atTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCAtatta                                                                     >  2:1249724/1‑148 (MQ=255)
                                                                                  cccGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGc                                                              <  1:1596576/148‑1 (MQ=255)
                                                                                      cgTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGt                                                          >  2:526604/1‑148 (MQ=255)
                                                                                        tctcGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTaa                                                        <  1:3244100/148‑1 (MQ=255)
                                                                                         ctcGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCTAAGCGCGCCTGCAGCAGATTATGTTTGCCCGTAAc                                                       >  2:2322272/1‑148 (MQ=255)
                                                                                         ctcGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAAc                                                       <  2:2355909/148‑1 (MQ=255)
                                                                                         ctcGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAAc                                                       >  1:242894/1‑148 (MQ=255)
                                                                                             cAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGcc                                                   <  2:296841/148‑1 (MQ=255)
                                                                                             cAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGca                                                   <  1:1366858/148‑2 (MQ=255)
                                                                                               aTCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAg                                                 >  1:2884034/1‑148 (MQ=255)
                                                                                                tCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGa                                                >  1:705879/1‑148 (MQ=255)
                                                                                                 cccTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGaa                                               <  2:72785/148‑1 (MQ=255)
                                                                                                   cTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGaaaa                                             <  1:1058555/148‑1 (MQ=255)
                                                                                                     tGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATg                                           <  2:1545792/148‑1 (MQ=255)
                                                                                                      gCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGt                                          <  2:1674811/148‑1 (MQ=255)
                                                                                                      gCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGt                                          >  2:596007/1‑148 (MQ=255)
                                                                                                      gCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGt                                          <  1:2041820/148‑1 (MQ=255)
                                                                                                          aaGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACAt                                      <  2:69227/148‑1 (MQ=255)
                                                                                                          aaGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACAt                                      >  2:717775/1‑148 (MQ=255)
                                                                                                          aaGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACAt                                      >  2:2680754/1‑148 (MQ=255)
                                                                                                          aaGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACAt                                      >  1:2407775/1‑148 (MQ=255)
                                                                                                          aaGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACAt                                      <  1:1482215/148‑1 (MQ=255)
                                                                                                           aGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATc                                     >  2:668375/1‑148 (MQ=255)
                                                                                                           aGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATc                                     <  1:1690966/148‑1 (MQ=255)
                                                                                                             aaCAGTCACTTGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATTGAATCAAACTACCTGAACAAACTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATccc                                   <  2:2489259/148‑1 (MQ=255)
                                                                                                             aaCAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATccc                                   >  1:1902871/1‑148 (MQ=255)
                                                                                                             aaCAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATccc                                   >  1:1729885/1‑148 (MQ=255)
                                                                                                                 gTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGgag                               >  2:2642161/1‑148 (MQ=255)
                                                                                                                  tCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGgaga                              <  2:2774902/148‑1 (MQ=255)
                                                                                                                  tCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGgaga                              <  1:1564633/148‑1 (MQ=255)
                                                                                                                  tCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGgaga                              <  1:3277505/148‑1 (MQ=255)
                                                                                                                   cACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGaa                             <  2:1920289/148‑1 (MQ=255)
                                                                                                                    aCTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAAt                            <  2:1124416/148‑1 (MQ=255)
                                                                                                                        gAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAATCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTa                        >  1:2601054/1‑148 (MQ=255)
                                                                                                                           aGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCg                     >  1:3165993/1‑148 (MQ=255)
                                                                                                                            gTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGt                    <  1:180128/148‑1 (MQ=255)
                                                                                                                                ggTCCGACAGCTTCTGGTGATGCTGGAAATGGTCAATGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCg                <  2:2034470/148‑1 (MQ=255)
                                                                                                                                  tCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTg              <  1:1345225/148‑1 (MQ=255)
                                                                                                                                   ccGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTgg             <  2:334350/148‑1 (MQ=255)
                                                                                                                                       cAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGAtt         >  1:1224899/1‑148 (MQ=255)
                                                                                                                                       cAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGAtt         >  1:569220/1‑148 (MQ=255)
                                                                                                                                        aGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTa        <  2:1410099/148‑1 (MQ=255)
                                                                                                                                         gCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTAc       >  1:1019122/1‑148 (MQ=255)
                                                                                                                                           ttCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTACTc     >  2:3030221/1‑148 (MQ=255)
                                                                                                                                            tCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTACTCg    >  1:3328166/1‑148 (MQ=255)
                                                                                                                                             cTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTACTCGt   <  2:246519/148‑1 (MQ=255)
                                                                                                                                             cTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAA‑TTTACCGTCTCGTGGGATTACTCGtt  <  2:2884034/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GTTTTATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTACTCGTT  >  CP009273/1871909‑1872198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGATGTTTTATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTACTCGTTATGCA  >  CP009273/1871905‑1872203
                                                                                                                                                     |                                                                                                                                                     
TGATGTTTTATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGT                                                                                                                                                       >  SRR19973066.1163390/1‑150 (MQ=60)
    GTTTTATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATG                                                                                                                                                   >  SRR19973066.341633/1‑150 (MQ=60)
         ATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGA                                                                                                                                              >  SRR19973066.547040/1‑150 (MQ=60)
         ATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGA                                                                                                                                              >  SRR19973066.865262/1‑150 (MQ=60)
         ATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGA                                                                                                                                              >  SRR19973066.2350192/1‑150 (MQ=60)
          TCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAA                                                                                                                                             >  SRR19973066.1202962/1‑150 (MQ=60)
          TCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAA                                                                                                                                             >  SRR19973066.1202980/1‑150 (MQ=60)
            ATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAAT                                                                                                                                           <  SRR19973066.1306199/150‑1 (MQ=60)
            ATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAAT                                                                                                                                           <  SRR19973066.1306221/150‑1 (MQ=60)
            ATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAAT                                                                                                                                           <  SRR19973066.966250/150‑1 (MQ=60)
            ATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAAT                                                                                                                                           <  SRR19973066.239567/150‑1 (MQ=60)
            ATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAAT                                                                                                                                           <  SRR19973066.239696/150‑1 (MQ=60)
                CTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTC                                                                                                                                       <  SRR19973066.478065/150‑1 (MQ=60)
                  GCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCCAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCAC                                                                                                                                     >  SRR19973066.3431073/1‑150 (MQ=60)
                  GCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCAC                                                                                                                                     <  SRR19973066.684755/150‑1 (MQ=60)
                  GCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCAC                                                                                                                                     >  SRR19973066.2950615/1‑150 (MQ=60)
                  GCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCAC                                                                                                                                     >  SRR19973066.2949932/1‑150 (MQ=60)
                   CAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACT                                                                                                                                    >  SRR19973066.2231492/1‑150 (MQ=60)
                   CAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACT                                                                                                                                    <  SRR19973066.3346697/150‑1 (MQ=60)
                   CAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACT                                                                                                                                    <  SRR19973066.3347245/150‑1 (MQ=60)
                   CAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACT                                                                                                                                    <  SRR19973066.3566746/150‑1 (MQ=60)
                   CAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACT                                                                                                                                    <  SRR19973066.3347378/150‑1 (MQ=60)
                    AAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTG                                                                                                                                   >  SRR19973066.3453270/1‑150 (MQ=60)
                      AGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGAT                                                                                                                                 <  SRR19973066.2518244/150‑1 (MQ=60)
                      AGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGAT                                                                                                                                 <  SRR19973066.2613438/150‑1 (MQ=60)
                        ATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATAT                                                                                                                               <  SRR19973066.2499623/150‑1 (MQ=60)
                        ATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATAT                                                                                                                               <  SRR19973066.2610835/150‑1 (MQ=60)
                        ATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATAT                                                                                                                               >  SRR19973066.1556853/1‑150 (MQ=60)
                        ATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATAT                                                                                                                               >  SRR19973066.3336896/1‑150 (MQ=60)
                           AAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGA                                                                                                                            >  SRR19973066.3243965/1‑150 (MQ=60)
                           AAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGA                                                                                                                            >  SRR19973066.3243976/1‑150 (MQ=60)
                           AAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGA                                                                                                                            >  SRR19973066.3243572/1‑150 (MQ=60)
                              TAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATC                                                                                                                         >  SRR19973066.923446/1‑150 (MQ=60)
                               AATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCA                                                                                                                        <  SRR19973066.1259539/150‑1 (MQ=60)
                               AATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCA                                                                                                                        <  SRR19973066.1259305/150‑1 (MQ=60)
                               AATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCA                                                                                                                        >  SRR19973066.1396954/1‑150 (MQ=60)
                               AATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCA                                                                                                                        >  SRR19973066.1079024/1‑150 (MQ=60)
                                 TCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAAC                                                                                                                      <  SRR19973066.1679099/150‑1 (MQ=60)
                                  CAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACC                                                                                                                     <  SRR19973066.2689482/150‑1 (MQ=60)
                                  CAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACC                                                                                                                     <  SRR19973066.1535821/150‑1 (MQ=60)
                                   AGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCT                                                                                                                    >  SRR19973066.2697427/1‑150 (MQ=60)
                                   AGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCT                                                                                                                    <  SRR19973066.2907601/150‑1 (MQ=60)
                                   AGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCT                                                                                                                    >  SRR19973066.2483374/1‑150 (MQ=60)
                                      ATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATCAACCTACC                                                                                                                 <  SRR19973066.2464853/150‑1 (MQ=60)
                                         TACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGA                                                                                                              >  SRR19973066.1576508/1‑150 (MQ=60)
                                         TACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGA                                                                                                              >  SRR19973066.1576443/1‑150 (MQ=60)
                                          ACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGAC                                                                                                             <  SRR19973066.2542173/150‑1 (MQ=60)
                                          ACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGAC                                                                                                             <  SRR19973066.1710875/150‑1 (MQ=60)
                                                CGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGT                                                                                                       <  SRR19973066.454487/150‑1 (MQ=60)
                                                 GTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTG                                                                                                      >  SRR19973066.2574945/1‑150 (MQ=60)
                                                  TATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGG                                                                                                     <  SRR19973066.1296262/150‑1 (MQ=60)
                                                  TATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGG                                                                                                     <  SRR19973066.1298790/150‑1 (MQ=60)
                                                  TATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGG                                                                                                     >  SRR19973066.1687014/1‑150 (MQ=60)
                                                   ATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGA                                                                                                    >  SRR19973066.3075921/1‑150 (MQ=60)
                                                   ATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGA                                                                                                    >  SRR19973066.438424/1‑150 (MQ=60)
                                                   ATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGA                                                                                                    <  SRR19973066.743922/150‑1 (MQ=60)
                                                    TCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCAGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGAT                                                                                                   >  SRR19973066.2905445/1‑150 (MQ=60)
                                                     CCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATG                                                                                                  <  SRR19973066.3451226/150‑1 (MQ=60)
                                                     CCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATG                                                                                                  >  SRR19973066.3244865/1‑150 (MQ=60)
                                                        GGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCG                                                                                               >  SRR19973066.73626/1‑150 (MQ=60)
                                                        GGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCG                                                                                               >  SRR19973066.276432/1‑150 (MQ=60)
                                                        GGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCG                                                                                               >  SRR19973066.73649/1‑150 (MQ=60)
                                                         GAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCATTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGA                                                                                              <  SRR19973066.2017368/150‑1 (MQ=60)
                                                          AGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAA                                                                                             >  SRR19973066.948615/1‑150 (MQ=60)
                                                          AGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAA                                                                                             <  SRR19973066.637414/150‑1 (MQ=60)
                                                          AGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAA                                                                                             >  SRR19973066.976005/1‑150 (MQ=60)
                                                             TGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCG                                                                                          <  SRR19973066.600674/150‑1 (MQ=60)
                                                             TGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCG                                                                                          <  SRR19973066.1318259/150‑1 (MQ=60)
                                                               TTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCG                                                                                        <  SRR19973066.1525062/150‑1 (MQ=60)
                                                                  CGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCT                                                                                     >  SRR19973066.1180853/1‑150 (MQ=60)
                                                                             AGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTA                                                                          >  SRR19973066.3295045/1‑150 (MQ=60)
                                                                                TTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGT                                                                       >  SRR19973066.2924342/1‑150 (MQ=60)
                                                                                  ATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTT                                                                     <  SRR19973066.1907700/150‑1 (MQ=60)
                                                                                     GCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCC                                                                  >  SRR19973066.2002756/1‑150 (MQ=60)
                                                                                      CCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCC                                                                 <  SRR19973066.1608141/150‑1 (MQ=60)
                                                                                           GTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAAC                                                            >  SRR19973066.244789/1‑150 (MQ=60)
                                                                                           GTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAAC                                                            >  SRR19973066.246055/1‑150 (MQ=60)
                                                                                            TCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACA                                                           >  SRR19973066.564560/1‑150 (MQ=60)
                                                                                            TCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACA                                                           <  SRR19973066.3265494/150‑1 (MQ=60)
                                                                                                GCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCA                                                       >  SRR19973066.575210/1‑150 (MQ=60)
                                                                                                 CAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAG                                                      >  SRR19973066.2903342/1‑150 (MQ=60)
                                                                                                 CAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCAAG                                                      <  SRR19973066.1376744/150‑1 (MQ=60)
                                                                                                  AATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGA                                                     >  SRR19973066.711097/1‑150 (MQ=60)
                                                                                                   ATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAA                                                    >  SRR19973066.1369943/1‑150 (MQ=60)
                                                                                                    TCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAA                                                   <  SRR19973066.2530867/150‑1 (MQ=60)
                                                                                                      CCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAAT                                                 <  SRR19973066.1701489/150‑1 (MQ=60)
                                                                                                       CTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATG                                                <  SRR19973066.1065940/150‑1 (MQ=60)
                                                                                                       CTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATG                                                <  SRR19973066.1066333/150‑1 (MQ=60)
                                                                                                       CTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATG                                                <  SRR19973066.2315081/150‑1 (MQ=60)
                                                                                                          GCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTAC                                             <  SRR19973066.2056037/150‑1 (MQ=60)
                                                                                                            CAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACAT                                           >  SRR19973066.2424079/1‑150 (MQ=60)
                                                                                                            CAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACAT                                           >  SRR19973066.2424072/1‑150 (MQ=60)
                                                                                                              AAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCC                                         <  SRR19973066.1492628/150‑1 (MQ=60)
                                                                                                               AGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCC                                        >  SRR19973066.2845883/1‑150 (MQ=60)
                                                                                                               AGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCC                                        <  SRR19973066.1703267/150‑1 (MQ=60)
                                                                                                               AGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCC                                        >  SRR19973066.1916439/1‑150 (MQ=60)
                                                                                                               AGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCC                                        >  SRR19973066.1742385/1‑150 (MQ=60)
                                                                                                               AGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCC                                        >  SRR19973066.1742394/1‑150 (MQ=60)
                                                                                                               AGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCC                                        >  SRR19973066.3517535/1‑150 (MQ=60)
                                                                                                                      TCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAAT                                 <  SRR19973066.3299028/150‑1 (MQ=60)
                                                                                                                      TCACTGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAAT                                 <  SRR19973066.1575904/150‑1 (MQ=60)
                                                                                                                          TGGAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAATCGCGCCTGCAGCATATTATGTTTGACCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTA                             >  SRR19973066.2618655/1‑150 (MQ=60)
                                                                                                                            GAAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACC                           <  SRR19973066.306172/150‑1 (MQ=60)
                                                                                                                             AAAGTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCG                          >  SRR19973066.3187083/1‑150 (MQ=60)
                                                                                                                                GTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCT                       <  SRR19973066.3367116/150‑1 (MQ=60)
                                                                                                                                GTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCT                       <  SRR19973066.181705/150‑1 (MQ=60)
                                                                                                                                GTCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCT                       <  SRR19973066.1736582/150‑1 (MQ=60)
                                                                                                                                 TCTGGTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTC                      <  SRR19973066.94575/150‑1 (MQ=60)
                                                                                                                                     GTCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGG                  <  SRR19973066.146800/150‑1 (MQ=60)
                                                                                                                                      TCCGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGG                 <  SRR19973066.1354979/150‑1 (MQ=60)
                                                                                                                                        CGACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGAT               <  SRR19973066.3176721/150‑1 (MQ=60)
                                                                                                                                         GACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATT              >  SRR19973066.573247/1‑150 (MQ=60)
                                                                                                                                         GACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATT              >  SRR19973066.1233571/1‑150 (MQ=60)
                                                                                                                                         GACAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATT              >  SRR19973066.3570868/1‑150 (MQ=60)
                                                                                                                                           CAGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTAC            >  SRR19973066.1026620/1‑150 (MQ=60)
                                                                                                                                            AGCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTACT           <  SRR19973066.2606000/150‑1 (MQ=60)
                                                                                                                                             GCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTACTC          <  SRR19973066.2196544/150‑1 (MQ=60)
                                                                                                                                             GCTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTACTC          >  SRR19973066.1318495/1‑150 (MQ=60)
                                                                                                                                              CTTCTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTACTCG         >  SRR19973066.3350279/1‑150 (MQ=60)
                                                                                                                                                 CTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTACTCGTTA      <  SRR19973066.917814/150‑1 (MQ=60)
                                                                                                                                                 CTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTACTCGTTA      <  SRR19973066.917901/150‑1 (MQ=60)
                                                                                                                                                 CTGGTGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTACTCGTTA      >  SRR19973066.1105301/1‑150 (MQ=60)
                                                                                                                                                     TGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTACTCGTTATGCA  >  SRR19973066.142409/1‑150 (MQ=60)
                                                                                                                                                     TGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTACTCGTTATGCA  >  SRR19973066.1316827/1‑150 (MQ=60)
                                                                                                                                                     TGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTACTCGTTATGCA  >  SRR19973066.1316148/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
TGATGTTTTATCATGCCTGCAAAGATTAAATAATCAGCATTTACCCGCCGTATCCTGGAGTTGTTCCGTGTCAGATCAGATTATCGCCCGCGTCTCGCAATCCCTTGCCAAAGAACAGTCACTGGAAAGTCTGGTCCGACAGCTTCTGGAGATGCTGGAAATGGTCACTGATATGGAATCAACCTACCTGACCAAAGTGGATGTCGAAGCGCGCCTGCAGCATATTATGTTTGCCCGTAACAGCCAGAAAATGTACATCCCGGAGAATTTTACCGTCTCGTGGGATTACTCGTTATGCA  >  CP009273/1871905‑1872203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑