Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,330,502 C→A 100% D800Y (GAT→TAT)  gyrA ← DNA gyrase (type II topoisomerase), subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,330,5020CA99.5% 665.6 / ‑4.3 207D800Y (GAT→TAT) gyrADNA gyrase (type II topoisomerase), subunit A
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base A (103/102);  total (104/103)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 207 total aligned reads displayed.

TCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTA  >  CP009273/2330360‑2330646
                                                                                                                                              |                                                                                                                                                
tCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGa                                                                                                                                             <  1:1468056/148‑1 (MQ=255)
tCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGa                                                                                                                                             <  1:3234995/148‑1 (MQ=255)
 cTTCCTCGTCAACCAGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGat                                                                                                                                            >  2:2493568/1‑148 (MQ=255)
  ttCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGatc                                                                                                                                           <  1:2626589/148‑1 (MQ=255)
    ccTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGatcat                                                                                                                                         <  1:2655685/148‑1 (MQ=255)
      tCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGAtcatca                                                                                                                                       >  2:170765/1‑148 (MQ=255)
      tCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGAtcatca                                                                                                                                       >  1:2251397/1‑148 (MQ=255)
         tCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGa                                                                                                                                    <  2:2944633/148‑1 (MQ=255)
          cAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGAt                                                                                                                                   >  1:2354537/1‑148 (MQ=255)
          cAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGAt                                                                                                                                   >  2:1193596/1‑148 (MQ=255)
           aaCCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATc                                                                                                                                  >  1:1763315/1‑148 (MQ=255)
             ccGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTg                                                                                                                                >  2:1665141/1‑148 (MQ=255)
             ccGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTg                                                                                                                                >  2:161361/1‑148 (MQ=255)
             ccGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTg                                                                                                                                >  2:2664487/1‑148 (MQ=255)
              cGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTgg                                                                                                                               <  1:2556037/148‑1 (MQ=255)
              cGGTTCAGCAACACGTTGCAGACCAACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCAGAAACGCGAGTACTTACCAGCGTACCGGCATAGGTGATCATCATCATCTgg                                                                                                                               >  2:3316791/1‑148 (MQ=255)
                gTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTc                                                                                                                             >  1:2894366/1‑148 (MQ=255)
                gTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTc                                                                                                                             >  1:480681/1‑148 (MQ=255)
                 ttCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCg                                                                                                                            <  1:2161080/148‑1 (MQ=255)
                   cAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCa                                                                                                                          <  1:1390403/148‑1 (MQ=255)
                   cAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCa                                                                                                                          >  2:343379/1‑148 (MQ=255)
                    aGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAg                                                                                                                         <  1:845833/148‑1 (MQ=255)
                    aGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAg                                                                                                                         <  1:2466036/148‑1 (MQ=255)
                     gCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGt                                                                                                                        >  1:1848164/1‑148 (MQ=255)
                     gCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGt                                                                                                                        >  1:2608539/1‑148 (MQ=255)
                     gCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGt                                                                                                                        >  2:722487/1‑148 (MQ=255)
                      cAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTc                                                                                                                       <  2:34075/148‑1 (MQ=255)
                          acGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCt                                                                                                                   <  2:3063004/148‑1 (MQ=255)
                              tGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCt                                                                                                               >  2:2398793/1‑148 (MQ=255)
                                 aGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTa                                                                                                            <  1:465054/148‑1 (MQ=255)
                                   aCCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTAcc                                                                                                          >  2:1437354/1‑148 (MQ=255)
                                   aCCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTAcc                                                                                                          >  2:601379/1‑148 (MQ=255)
                                       aCTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCgcgc                                                                                                      >  1:449295/1‑148 (MQ=255)
                                          aCGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCcaa                                                                                                   >  2:2267794/1‑148 (MQ=255)
                                            gTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCcaaca                                                                                                 <  1:3227661/148‑1 (MQ=255)
                                             ttttCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCcaacaa                                                                                                >  1:3177329/1‑148 (MQ=255)
                                              tttCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCaacaac                                                                                               <  1:2358747/148‑1 (MQ=255)
                                              tttCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCaacaac                                                                                               >  1:1874715/1‑148 (MQ=255)
                                               ttCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACt                                                                                              >  1:1032377/1‑148 (MQ=255)
                                                  aTCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTaaa                                                                                           <  1:2583041/148‑1 (MQ=255)
                                                  aTCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTaaa                                                                                           <  2:2306408/148‑1 (MQ=255)
                                                       ccGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGtt                                                                                      <  2:676546/148‑1 (MQ=255)
                                                         gCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCACCAACTAAACCGTTAc                                                                                    >  2:2189790/1‑148 (MQ=255)
                                                         gCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTAc                                                                                    >  2:621232/1‑148 (MQ=255)
                                                              aCGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCg                                                                               >  1:2013184/1‑148 (MQ=255)
                                                                 gATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTa                                                                            >  1:542103/1‑148 (MQ=255)
                                                                  aTGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTaa                                                                           <  1:1326783/148‑1 (MQ=255)
                                                                    gAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAAcc                                                                         >  1:2910235/1‑148 (MQ=255)
                                                                     aGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCt                                                                        <  1:2181485/148‑1 (MQ=255)
                                                                       gATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTg                                                                      <  1:2851908/148‑1 (MQ=255)
                                                                       gATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTg                                                                      <  2:3488198/148‑1 (MQ=255)
                                                                           aCGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATgg                                                                  <  2:751117/148‑1 (MQ=255)
                                                                             gCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGgag                                                                <  2:1227331/148‑1 (MQ=255)
                                                                              cccTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGgaga                                                               <  2:3511520/148‑1 (MQ=255)
                                                                               ccTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGAt                                                              <  1:1107536/148‑1 (MQ=255)
                                                                                cTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGTTCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCTCCAACAACTAAACCGTTTCGTTCGGTAACCTTGATGGAGATa                                                             <  2:1667642/148‑1 (MQ=255)
                                                                                cTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGGGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATa                                                             <  2:3156474/148‑1 (MQ=255)
                                                                                   ggTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAAcc                                                          <  1:722487/148‑1 (MQ=255)
                                                                                      gtTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCt                                                       <  2:477103/148‑1 (MQ=255)
                                                                                      gtTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCt                                                       <  2:3238601/148‑1 (MQ=255)
                                                                                          cGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTtcg                                                   <  1:3311557/148‑1 (MQ=255)
                                                                                           ggCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTtcgt                                                  <  2:703667/148‑1 (MQ=255)
                                                                                            gCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTtcgtc                                                 >  2:1582663/1‑148 (MQ=255)
                                                                                               cACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAACCCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCa                                              <  2:3144703/148‑1 (MQ=255)
                                                                                                  gATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCAcgc                                           >  2:3084916/1‑148 (MQ=255)
                                                                                                   aTGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCAcgcg                                          <  2:2481352/148‑1 (MQ=255)
                                                                                                   aTGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCAcgcg                                          >  1:711363/1‑148 (MQ=255)
                                                                                                   aTGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCAcgcg                                          <  1:2698491/148‑1 (MQ=255)
                                                                                                   aTGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCAcgcg                                          <  1:914513/148‑1 (MQ=255)
                                                                                                    tGCTTATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGa                                         >  2:2609273/1‑148 (MQ=255)
                                                                                                    tGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGa                                         <  1:161361/148‑1 (MQ=255)
                                                                                                        gATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACttg                                     >  1:1274556/1‑148 (MQ=255)
                                                                                                         aTTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACttgg                                    <  2:1387/148‑1 (MQ=255)
                                                                                                          tttCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACttggt                                   <  1:1084843/148‑1 (MQ=255)
                                                                                                           ttCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACttggtt                                  >  1:3022625/1‑148 (MQ=255)
                                                                                                           ttCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACttggtt                                  >  1:703516/1‑148 (MQ=255)
                                                                                                                  aCGCGAGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTAtt                           <  1:3500315/148‑1 (MQ=255)
                                                                                                                  aCGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTAtt                           <  2:1874715/148‑1 (MQ=255)
                                                                                                                  aCGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTAtt                           <  2:617085/148‑1 (MQ=255)
                                                                                                                   cgcgAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTc                          <  1:286857/148‑1 (MQ=255)
                                                                                                                              acCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCgg               <  2:1032377/148‑1 (MQ=255)
                                                                                                                                cAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTa             <  2:1816696/148‑1 (MQ=255)
                                                                                                                                  gCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACg           >  1:1102486/1‑148 (MQ=255)
                                                                                                                                  gCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACg           <  1:1437344/148‑1 (MQ=255)
                                                                                                                                  gCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACg           >  1:3069237/1‑148 (MQ=255)
                                                                                                                                  gCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACg           >  1:2033827/1‑148 (MQ=255)
                                                                                                                                   cGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGt          >  2:2371460/1‑148 (MQ=255)
                                                                                                                                   cGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGt          >  1:746792/1‑148 (MQ=255)
                                                                                                                                   agTACCGGCATAGGTGATCATCATGATATGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGTTAACATTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGGTGGGTATTCCGCCACTGCGGTACGt          >  1:280218/2‑148 (MQ=255)
                                                                                                                                    gTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGtt         <  2:2721797/148‑1 (MQ=255)
                                                                                                                                     tACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGttt        <  1:612697/148‑1 (MQ=255)
                                                                                                                                     tACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGttt        <  1:2398793/148‑1 (MQ=255)
                                                                                                                                     tACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTGGGGTATTCCGCCACTGCGGTACGttt        >  1:2330775/1‑148 (MQ=255)
                                                                                                                                      aCCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTa       >  2:2811475/1‑148 (MQ=255)
                                                                                                                                      aCCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTa       <  2:1848164/148‑1 (MQ=255)
                                                                                                                                        cGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTAcc     >  1:3395663/1‑148 (MQ=255)
                                                                                                                                         ggCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCg    <  2:107065/148‑1 (MQ=255)
                                                                                                                                           cATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTa  >  2:3068036/1‑148 (MQ=255)
                                                                                                                                           cATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTa  <  1:576271/148‑1 (MQ=255)
                                                                                                                                           cATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTa  <  1:1036347/148‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                
TCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTA  >  CP009273/2330360‑2330646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATCCAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACCGT  >  CP009273/2330353‑2330651
                                                                                                                                                     |                                                                                                                                                     
ATCCAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATA                                                                                                                                                       <  SRR19973067.1838727/150‑1 (MQ=60)
ATCCAGATCGTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATA                                                                                                                                                       <  SRR19973067.1840621/150‑1 (MQ=60)
 TCCAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGTTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAG                                                                                                                                                      <  SRR19973067.1074965/150‑1 (MQ=60)
   CAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGT                                                                                                                                                    <  SRR19973067.107894/150‑1 (MQ=60)
       TCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATC                                                                                                                                                <  SRR19973067.1477846/150‑1 (MQ=60)
       TCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATC                                                                                                                                                <  SRR19973067.3255246/150‑1 (MQ=60)
       TCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATC                                                                                                                                                <  SRR19973067.3255287/150‑1 (MQ=60)
       TCGTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATC                                                                                                                                                <  SRR19973067.2193275/150‑1 (MQ=60)
         TTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCAT                                                                                                                                              <  SRR19973067.2582144/150‑1 (MQ=60)
         TTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCAT                                                                                                                                              <  SRR19973067.2643614/150‑1 (MQ=60)
           CCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCA                                                                                                                                            >  SRR19973067.2266344/1‑150 (MQ=60)
           CCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCA                                                                                                                                            <  SRR19973067.2672788/150‑1 (MQ=60)
              CGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGA                                                                                                                                         >  SRR19973067.108119/1‑150 (MQ=60)
               GTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGAT                                                                                                                                        <  SRR19973067.2883489/150‑1 (MQ=60)
               GTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGAT                                                                                                                                        <  SRR19973067.1097832/150‑1 (MQ=60)
               GTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGAT                                                                                                                                        >  SRR19973067.2369899/1‑150 (MQ=60)
               GTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGAT                                                                                                                                        <  SRR19973067.604876/150‑1 (MQ=60)
               GTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGAT                                                                                                                                        >  SRR19973067.571763/1‑150 (MQ=60)
                TCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATC                                                                                                                                       >  SRR19973067.1775310/1‑150 (MQ=60)
                     CGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTC                                                                                                                                  >  SRR19973067.483997/1‑150 (MQ=60)
                     CGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTC                                                                                                                                  <  SRR19973067.2572748/150‑1 (MQ=60)
                     CGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTC                                                                                                                                  >  SRR19973067.1420003/1‑150 (MQ=60)
                     CGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTC                                                                                                                                  >  SRR19973067.2912847/1‑150 (MQ=60)
                      GGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCG                                                                                                                                 <  SRR19973067.2078501/150‑1 (MQ=60)
                        TTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCA                                                                                                                               <  SRR19973067.2175525/150‑1 (MQ=60)
                          CAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGT                                                                                                                             <  SRR19973067.1399852/150‑1 (MQ=60)
                          CAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGT                                                                                                                             >  SRR19973067.1860760/1‑150 (MQ=60)
                          CAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGT                                                                                                                             >  SRR19973067.2625482/1‑150 (MQ=60)
                           AGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTC                                                                                                                            >  SRR19973067.2566187/1‑150 (MQ=60)
                           AGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTC                                                                                                                            <  SRR19973067.2482255/150‑1 (MQ=60)
                           AGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTC                                                                                                                            <  SRR19973067.2643932/150‑1 (MQ=60)
                           AGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTC                                                                                                                            <  SRR19973067.851562/150‑1 (MQ=60)
                            GCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCA                                                                                                                           <  SRR19973067.309225/150‑1 (MQ=60)
                                        AGACCCACTACGTTTTCATCTTCCGCAGTACGTATGACGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACAAGCGAACCGGCATAGGTGATAAGAATGATCTGGTCGCAGTCAGCTACCTGTAAC                                                                                                               >  SRR19973067.306664/1‑150 (MQ=60)
                                        AGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACC                                                                                                               <  SRR19973067.468269/150‑1 (MQ=60)
                                            CCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGC                                                                                                           >  SRR19973067.2253440/1‑150 (MQ=60)
                                            CCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGC                                                                                                           >  SRR19973067.452415/1‑150 (MQ=60)
                                             CACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCC                                                                                                          >  SRR19973067.738706/1‑150 (MQ=60)
                                             CACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCC                                                                                                          >  SRR19973067.2400047/1‑150 (MQ=60)
                                                 ACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACA                                                                                                      >  SRR19973067.73023/1‑150 (MQ=60)
                                                 ACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACA                                                                                                      >  SRR19973067.69720/1‑150 (MQ=60)
                                                  CGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAA                                                                                                     >  SRR19973067.3197434/1‑150 (MQ=60)
                                                  CGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAA                                                                                                     >  SRR19973067.802773/1‑150 (MQ=60)
                                                   GTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAAC                                                                                                    <  SRR19973067.3247926/150‑1 (MQ=60)
                                                   GTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTAACGCGCCAACAAC                                                                                                    >  SRR19973067.1887500/1‑150 (MQ=60)
                                                    TTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACT                                                                                                   >  SRR19973067.1039517/1‑150 (MQ=60)
                                                    TTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACT                                                                                                   >  SRR19973067.1039487/1‑150 (MQ=60)
                                                    TTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACT                                                                                                   >  SRR19973067.4605/1‑150 (MQ=60)
                                                    TTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACT                                                                                                   >  SRR19973067.4846/1‑150 (MQ=60)
                                                     TTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTA                                                                                                  <  SRR19973067.2374126/150‑1 (MQ=60)
                                                     TTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTA                                                                                                  >  SRR19973067.1412/1‑150 (MQ=60)
                                                      TTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAA                                                                                                 <  SRR19973067.2462418/150‑1 (MQ=60)
                                                         ATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACC                                                                                              <  SRR19973067.2599865/150‑1 (MQ=60)
                                                         ATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACC                                                                                              >  SRR19973067.2810082/1‑150 (MQ=60)
                                                         ATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACC                                                                                              >  SRR19973067.35685/1‑150 (MQ=60)
                                                         ATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACC                                                                                              >  SRR19973067.2957384/1‑150 (MQ=60)
                                                            TTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTT                                                                                           >  SRR19973067.3444631/1‑150 (MQ=60)
                                                              CCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTAC                                                                                         <  SRR19973067.3213365/150‑1 (MQ=60)
                                                               CGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACG                                                                                        >  SRR19973067.1621643/1‑150 (MQ=60)
                                                                   GTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCG                                                                                    >  SRR19973067.2027059/1‑150 (MQ=60)
                                                                   GTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCG                                                                                    >  SRR19973067.2026730/1‑150 (MQ=60)
                                                                   GTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCG                                                                                    >  SRR19973067.2026724/1‑150 (MQ=60)
                                                                     ACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGT                                                                                  <  SRR19973067.2896699/150‑1 (MQ=60)
                                                                      CGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTA                                                                                 >  SRR19973067.545706/1‑150 (MQ=60)
                                                                         ATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACC                                                                              <  SRR19973067.1335839/150‑1 (MQ=60)
                                                                         ATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACC                                                                              >  SRR19973067.2928761/1‑150 (MQ=60)
                                                                         ATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACC                                                                              >  SRR19973067.2928776/1‑150 (MQ=60)
                                                                           GAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTT                                                                            <  SRR19973067.1836986/150‑1 (MQ=60)
                                                                            AGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTG                                                                           <  SRR19973067.2196074/150‑1 (MQ=60)
                                                                            AGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTG                                                                           >  SRR19973067.873474/1‑150 (MQ=60)
                                                                              GATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGAT                                                                         <  SRR19973067.2870200/150‑1 (MQ=60)
                                                                                      CCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAA                                                                 <  SRR19973067.1115117/150‑1 (MQ=60)
                                                                                      CCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAA                                                                 <  SRR19973067.1115059/150‑1 (MQ=60)
                                                                                         GGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCC                                                              <  SRR19973067.429411/150‑1 (MQ=60)
                                                                                         GGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCC                                                              <  SRR19973067.2629705/150‑1 (MQ=60)
                                                                                          GGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCC                                                             <  SRR19973067.727510/150‑1 (MQ=60)
                                                                                              TTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTC                                                         <  SRR19973067.2601694/150‑1 (MQ=60)
                                                                                                 CGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTC                                                      >  SRR19973067.2851528/1‑150 (MQ=60)
                                                                                                 CGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTC                                                      <  SRR19973067.3332474/150‑1 (MQ=60)
                                                                                                 CGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTC                                                      <  SRR19973067.2673319/150‑1 (MQ=60)
                                                                                                        CGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCG                                               >  SRR19973067.716291/1‑150 (MQ=60)
                                                                                                        CGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCG                                               >  SRR19973067.716271/1‑150 (MQ=60)
                                                                                                          ATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGAC                                             >  SRR19973067.940116/1‑150 (MQ=60)
                                                                                                          ATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGAC                                             <  SRR19973067.920604/150‑1 (MQ=60)
                                                                                                          ATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGAC                                             >  SRR19973067.1874905/1‑150 (MQ=60)
                                                                                                          ATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGAC                                             <  SRR19973067.2715851/150‑1 (MQ=60)
                                                                                                           TGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACT                                            <  SRR19973067.162696/150‑1 (MQ=60)
                                                                                                           TGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACT                                            <  SRR19973067.1676625/150‑1 (MQ=60)
                                                                                                             CTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTG                                          >  SRR19973067.2334243/1‑150 (MQ=60)
                                                                                                             CTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTG                                          >  SRR19973067.1283229/1‑150 (MQ=60)
                                                                                                             CTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTG                                          >  SRR19973067.1286501/1‑150 (MQ=60)
                                                                                                                ATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTT                                       >  SRR19973067.3041869/1‑150 (MQ=60)
                                                                                                                ATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGGT                                       >  SRR19973067.708381/1‑150 (MQ=60)
                                                                                                                 TTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTG                                      <  SRR19973067.1092265/150‑1 (MQ=60)
                                                                                                                         ACGCGAGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCC                              <  SRR19973067.3522511/150‑1 (MQ=60)
                                                                                                                         ACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCC                              <  SRR19973067.326820/150‑1 (MQ=60)
                                                                                                                          CGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCG                             <  SRR19973067.288725/150‑1 (MQ=60)
                                                                                                                              AGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCAC                         >  SRR19973067.2711655/1‑150 (MQ=60)
                                                                                                                               GTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACT                        <  SRR19973067.236984/150‑1 (MQ=60)
                                                                                                                               GTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACT                        <  SRR19973067.2278628/150‑1 (MQ=60)
                                                                                                                                   GTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGG                    >  SRR19973067.50084/1‑150 (MQ=60)
                                                                                                                                   GTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGG                    >  SRR19973067.2025936/1‑150 (MQ=60)
                                                                                                                                       CAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACG                >  SRR19973067.2047451/1‑150 (MQ=60)
                                                                                                                                       CAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACG                >  SRR19973067.1109984/1‑150 (MQ=60)
                                                                                                                                       CAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACG                >  SRR19973067.3088775/1‑150 (MQ=60)
                                                                                                                                       CAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACG                >  SRR19973067.1109985/1‑150 (MQ=60)
                                                                                                                                        AGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGT               >  SRR19973067.751977/1‑150 (MQ=60)
                                                                                                                                        AGAGTACCGGCATAGGTGATCATCATGATATGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGTTAACATTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGGTGGGTATTCCGCCACTGCGGTACGT               >  SRR19973067.282064/1‑150 (MQ=60)
                                                                                                                                         GCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTT              <  SRR19973067.1447025/150‑1 (MQ=60)
                                                                                                                                         GCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTT              <  SRR19973067.1447015/150‑1 (MQ=60)
                                                                                                                                          CGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTGGGGTATTCCGCCACTGCGGTACGTTT             >  SRR19973067.2346067/1‑150 (MQ=60)
                                                                                                                                            TACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTAC           <  SRR19973067.2414430/150‑1 (MQ=60)
                                                                                                                                            TACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTAC           <  SRR19973067.616675/150‑1 (MQ=60)
                                                                                                                                             ACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACC          >  SRR19973067.3417267/1‑150 (MQ=60)
                                                                                                                                             ACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACC          <  SRR19973067.2370315/150‑1 (MQ=60)
                                                                                                                                             ACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACC          >  SRR19973067.3417242/1‑150 (MQ=60)
                                                                                                                                                  CATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAAC     >  SRR19973067.1105497/1‑150 (MQ=60)
                                                                                                                                                  CATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAAC     <  SRR19973067.579984/150‑1 (MQ=60)
                                                                                                                                                  CATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAAC     <  SRR19973067.1043476/150‑1 (MQ=60)
                                                                                                                                                   ATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACC    <  SRR19973067.2008268/150‑1 (MQ=60)
                                                                                                                                                     AGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACCGT  >  SRR19973067.1953059/1‑150 (MQ=60)
                                                                                                                                                     AGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACCGT  >  SRR19973067.1954506/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
ATCCAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACCGT  >  CP009273/2330353‑2330651

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑