Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F204 I0 R1 1155 255.8 8356456 95.7% 7997128 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,330,502 C→A 100% D800Y (GAT→TAT)  gyrA ← DNA gyrase (type II topoisomerase), subunit A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,330,5020CA96.0% 793.8 / 22.8 249D800Y (GAT→TAT) gyrADNA gyrase (type II topoisomerase), subunit A
Reads supporting (aligned to +/- strand):  ref base C (8/2);  new base A (121/118);  total (129/120)
Fisher's exact test for biased strand distribution p-value = 1.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.54e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 249 total aligned reads displayed.

CCAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAAC  >  CP009273/2330355‑2330648
                                                                                                                                                   |                                                                                                                                                  
ccAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAgg                                                                                                                                                  >  1:2102799/1‑150 (MQ=255)
ccAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAgg                                                                                                                                                  >  1:923228/1‑150 (MQ=255)
 cAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGt                                                                                                                                                 >  2:552267/1‑150 (MQ=255)
  aGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTg                                                                                                                                                >  1:2883817/1‑150 (MQ=255)
  aGATCTTCCTCGTAAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTg                                                                                                                                                <  1:4166198/150‑1 (MQ=255)
     tCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGatc                                                                                                                                             >  2:847592/1‑150 (MQ=255)
          cTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATcatcat                                                                                                                                        <  1:566505/150‑1 (MQ=255)
           tCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATg                                                                                                                                       <  1:2737793/150‑1 (MQ=255)
             gTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGAt                                                                                                                                     <  2:1280223/150‑1 (MQ=255)
              tCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATc                                                                                                                                    <  2:4107405/150‑1 (MQ=255)
               cAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCt                                                                                                                                   <  2:721727/150‑1 (MQ=255)
               cAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCt                                                                                                                                   <  1:3874248/150‑1 (MQ=255)
                aaCCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTg                                                                                                                                  <  1:1722801/150‑1 (MQ=255)
                   cGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTACGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTc                                                                                                                               <  1:3568121/150‑1 (MQ=255)
                    ggTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCg                                                                                                                              <  2:1748181/150‑1 (MQ=255)
                    ggTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCg                                                                                                                              <  2:1748282/150‑1 (MQ=255)
                     gTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGc                                                                                                                             >  2:58599/1‑150 (MQ=255)
                      ttCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCa                                                                                                                            <  2:4084642/150‑1 (MQ=255)
                       tCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAg                                                                                                                           >  2:3089639/1‑150 (MQ=255)
                        cAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGt                                                                                                                          <  2:3988526/150‑1 (MQ=255)
                         aGCAACTCGTTGCAGACCCAATACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACTATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATAATCATGATCTGGTCGCAGTc                                                                                                                         <  1:337226/150‑1 (MQ=255)
                         aGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTc                                                                                                                         >  2:4042231/1‑150 (MQ=255)
                            aaCACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTCCGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATc                                                                                                                      >  2:1929177/1‑150 (MQ=255)
                             acacGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCt                                                                                                                     >  1:501437/1‑150 (MQ=255)
                             acacGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCt                                                                                                                     <  1:2141571/150‑1 (MQ=255)
                              cacGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTa                                                                                                                    <  1:3602116/150‑1 (MQ=255)
                              cacGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTa                                                                                                                    <  1:1392954/150‑1 (MQ=255)
                               acGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTAc                                                                                                                   <  2:4162298/150‑1 (MQ=255)
                                 gTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCt                                                                                                                 <  1:3336930/150‑1 (MQ=255)
                                      aGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTAcc                                                                                                            >  1:3814725/1‑150 (MQ=255)
                                      aGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTAcc                                                                                                            >  1:1192486/1‑150 (MQ=255)
                                      aGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTAcc                                                                                                            <  1:1393586/150‑1 (MQ=255)
                                       gACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACcg                                                                                                           >  2:1337067/1‑150 (MQ=255)
                                          ccACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCgcgc                                                                                                        >  1:647955/1‑150 (MQ=255)
                                            aCTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCca                                                                                                      <  2:3866587/150‑1 (MQ=255)
                                            aCTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCca                                                                                                      <  2:3866346/150‑1 (MQ=255)
                                              tACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCcaac                                                                                                    >  1:1229483/1‑150 (MQ=255)
                                                cGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCcaacaa                                                                                                  >  1:4038693/1‑150 (MQ=255)
                                                  ttttCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACt                                                                                                >  2:1288988/1‑150 (MQ=255)
                                                  ttttCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACt                                                                                                <  2:2009604/150‑1 (MQ=255)
                                                   tttCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTa                                                                                               >  1:3419648/1‑150 (MQ=255)
                                                    ttCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTaa                                                                                              <  1:798104/150‑1 (MQ=255)
                                                    ttCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTaa                                                                                              >  2:2421315/1‑150 (MQ=255)
                                                      cATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAAc                                                                                            >  1:1073843/1‑150 (MQ=255)
                                                       aTCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAAcc                                                                                           <  2:2637880/150‑1 (MQ=255)
                                                         cTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGt                                                                                         >  1:3951156/1‑150 (MQ=255)
                                                            ccGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTAc                                                                                      <  1:4096870/150‑1 (MQ=255)
                                                            ccGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTAc                                                                                      <  2:3758490/150‑1 (MQ=255)
                                                              gCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGt                                                                                    >  1:3110710/1‑150 (MQ=255)
                                                               cAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGtt                                                                                   <  1:3236061/150‑1 (MQ=255)
                                                                aGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTc                                                                                  <  2:3590488/150‑1 (MQ=255)
                                                                   aCGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGt                                                                               >  1:1014874/1‑150 (MQ=255)
                                                                      gATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAAc                                                                            <  2:3912768/150‑1 (MQ=255)
                                                                      gATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAAc                                                                            >  1:72633/1‑150 (MQ=255)
                                                                      gATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAAc                                                                            <  1:2696310/150‑1 (MQ=255)
                                                                       aTGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAAcc                                                                           >  1:3571720/1‑150 (MQ=255)
                                                                       aTGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAAcc                                                                           >  1:228336/1‑150 (MQ=255)
                                                                        tGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGAACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTACCCt                                                                          >  2:3802435/1‑150 (MQ=255)
                                                                         gAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCtt                                                                         <  1:2077349/150‑1 (MQ=255)
                                                                          aGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTg                                                                        <  1:2122143/150‑1 (MQ=255)
                                                                              tCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATgg                                                                    <  2:3933940/150‑1 (MQ=255)
                                                                              tCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATgg                                                                    >  1:1622702/1‑150 (MQ=255)
                                                                               cACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGga                                                                   >  2:288013/1‑150 (MQ=255)
                                                                                  gCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGAt                                                                <  1:1093201/150‑1 (MQ=255)
                                                                                  gCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGAt                                                                <  2:3444339/150‑1 (MQ=255)
                                                                                    ccTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATaa                                                              <  1:2872169/150‑1 (MQ=255)
                                                                                      tGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACACCTAAACCGTTACGTTCGGTAACCTTGATGGAGATAAcc                                                            <  2:3912006/150‑1 (MQ=255)
                                                                                       gggTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAAccc                                                           <  1:1575039/150‑1 (MQ=255)
                                                                                        ggTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAAcccc                                                          >  1:17497/1‑150 (MQ=255)
                                                                                         gagtTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCt                                                         <  1:2135416/148‑1 (MQ=255)
                                                                                          tgtTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCtt                                                        >  1:2543630/1‑150 (MQ=255)
                                                                                          tgtTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCtt                                                        <  1:3597945/150‑1 (MQ=255)
                                                                                               cGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTtcgtc                                                   <  2:2083704/150‑1 (MQ=255)
                                                                                                ggCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTtcgtcg                                                  <  1:2610716/150‑1 (MQ=255)
                                                                                                    cACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCAcg                                              >  1:3790231/1‑150 (MQ=255)
                                                                                                     aCGATGCTGATTTACGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCAcgc                                             >  2:2121730/1‑150 (MQ=255)
                                                                                                      cGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCAcgcg                                            >  1:3953607/1‑150 (MQ=255)
                                                                                                           cTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACttg                                       >  1:2668165/1‑150 (MQ=255)
                                                                                                            tGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACttgg                                      >  2:863918/1‑150 (MQ=255)
                                                                                                              aTTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACttggtt                                    >  1:1795915/1‑150 (MQ=255)
                                                                                                               tttCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACttggttg                                   <  2:1583212/150‑1 (MQ=255)
                                                                                                                  ccGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGt                                <  2:1176617/150‑1 (MQ=255)
                                                                                                                       aCGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTcc                           <  2:1104442/150‑1 (MQ=255)
                                                                                                                           gAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCa                       <  2:647888/150‑1 (MQ=255)
                                                                                                                            aGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCAc                      >  1:202063/1‑150 (MQ=255)
                                                                                                                            aGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGAGAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCAc                      >  2:437774/1‑150 (MQ=255)
                                                                                                                             gtacgtacCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACt                     <  1:1326883/150‑1 (MQ=255)
                                                                                                                                cgtacCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACGTGGTTGGGTATTCCGCCACTGCg                  >  1:3660150/1‑150 (MQ=255)
                                                                                                                                 gtacCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCgg                 >  2:1546259/1‑150 (MQ=255)
                                                                                                                                     cAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACg             >  1:1319820/1‑150 (MQ=255)
                                                                                                                                     cAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACg             >  1:3280688/1‑150 (MQ=255)
                                                                                                                                     cAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACg             >  1:2325123/1‑150 (MQ=255)
                                                                                                                                      aGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGt            >  1:2672825/1‑150 (MQ=255)
                                                                                                                                      aGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGt            >  2:740953/1‑150 (MQ=255)
                                                                                                                                      aGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGt            >  2:861284/1‑150 (MQ=255)
                                                                                                                                        cGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGttt          <  1:1885756/150‑1 (MQ=255)
                                                                                                                                          tACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTAc        >  2:2935297/1‑150 (MQ=255)
                                                                                                                                            ccGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACACCTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCg      <  2:519475/150‑1 (MQ=255)
                                                                                                                                            ccGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCg      >  2:3337500/1‑150 (MQ=255)
                                                                                                                                             cGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGt     >  2:1584053/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CCAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAAC  >  CP009273/2330355‑2330648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCCAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACCGT  >  CP009273/2330354‑2330651
                                                                                                                                                    |                                                                                                                                                     
TCCAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAG                                                                                                                                                      >  SRR19973074.550686/1‑150 (MQ=60)
 CCAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGG                                                                                                                                                     >  SRR19973074.924987/1‑150 (MQ=60)
 CCAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGG                                                                                                                                                     >  SRR19973074.3936907/1‑150 (MQ=60)
 CCAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGG                                                                                                                                                     >  SRR19973074.2106744/1‑150 (MQ=60)
 CCAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGG                                                                                                                                                     >  SRR19973074.2106739/1‑150 (MQ=60)
 CCAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGAATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGG                                                                                                                                                     >  SRR19973074.924933/1‑150 (MQ=60)
   AGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTG                                                                                                                                                   <  SRR19973074.2264517/150‑1 (MQ=60)
   AGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTG                                                                                                                                                   >  SRR19973074.2889169/1‑150 (MQ=60)
   AGATCTTCCTCGTAAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTG                                                                                                                                                   <  SRR19973074.4173859/150‑1 (MQ=60)
    GATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGA                                                                                                                                                  <  SRR19973074.696155/150‑1 (MQ=60)
           CTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCAT                                                                                                                                           <  SRR19973074.2654632/150‑1 (MQ=60)
           CTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCAT                                                                                                                                           <  SRR19973074.567619/150‑1 (MQ=60)
            TCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATG                                                                                                                                          <  SRR19973074.2742843/150‑1 (MQ=60)
            TCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATG                                                                                                                                          <  SRR19973074.1732367/150‑1 (MQ=60)
              GTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGAT                                                                                                                                        >  SRR19973074.2089440/1‑150 (MQ=60)
               TCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATC                                                                                                                                       <  SRR19973074.1915760/150‑1 (MQ=60)
                CAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCT                                                                                                                                      <  SRR19973074.3881339/150‑1 (MQ=60)
                 AACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTG                                                                                                                                     <  SRR19973074.3022373/150‑1 (MQ=60)
                 AACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTG                                                                                                                                     <  SRR19973074.1725999/150‑1 (MQ=60)
                 AACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTG                                                                                                                                     >  SRR19973074.257637/1‑150 (MQ=60)
                  ACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGG                                                                                                                                    <  SRR19973074.3957265/150‑1 (MQ=60)
                    CGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTACGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTC                                                                                                                                  <  SRR19973074.3574702/150‑1 (MQ=60)
                      GTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGC                                                                                                                                >  SRR19973074.3580594/1‑150 (MQ=60)
                        TCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAG                                                                                                                              <  SRR19973074.3813966/150‑1 (MQ=60)
                         CAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGT                                                                                                                             >  SRR19973074.3966421/1‑150 (MQ=60)
                          AGCAACTCGTTGCAGACCCAATACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACTATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATAATCATGATCTGGTCGCAGTC                                                                                                                            <  SRR19973074.337837/150‑1 (MQ=60)
                          AGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTC                                                                                                                            <  SRR19973074.337834/150‑1 (MQ=60)
                              ACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCT                                                                                                                        <  SRR19973074.2145590/150‑1 (MQ=60)
                              ACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCT                                                                                                                        >  SRR19973074.502443/1‑150 (MQ=60)
                              ACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGCGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCT                                                                                                                        <  SRR19973074.2145581/150‑1 (MQ=60)
                               CACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTA                                                                                                                       <  SRR19973074.1395511/150‑1 (MQ=60)
                               CACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTA                                                                                                                       <  SRR19973074.3608770/150‑1 (MQ=60)
                                 CGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACC                                                                                                                     >  SRR19973074.158655/1‑150 (MQ=60)
                                  GTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTTTTACGTCCCACTATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCT                                                                                                                    <  SRR19973074.1755304/150‑1 (MQ=60)
                                  GTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCT                                                                                                                    <  SRR19973074.3343123/150‑1 (MQ=60)
                                  GTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCT                                                                                                                    <  SRR19973074.733491/150‑1 (MQ=60)
                                  GTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCT                                                                                                                    <  SRR19973074.733524/150‑1 (MQ=60)
                                   TTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTG                                                                                                                   <  SRR19973074.204053/150‑1 (MQ=60)
                                      CAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTAC                                                                                                                >  SRR19973074.606997/1‑150 (MQ=60)
                                       AGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACC                                                                                                               >  SRR19973074.3821690/1‑150 (MQ=60)
                                       AGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACC                                                                                                               <  SRR19973074.1396147/150‑1 (MQ=60)
                                       AGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACC                                                                                                               >  SRR19973074.1194760/1‑150 (MQ=60)
                                       AGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACC                                                                                                               >  SRR19973074.2288285/1‑150 (MQ=60)
                                       AGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGAGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACC                                                                                                               <  SRR19973074.1396123/150‑1 (MQ=60)
                                          CCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCG                                                                                                            >  SRR19973074.1299308/1‑150 (MQ=60)
                                           CCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGC                                                                                                           >  SRR19973074.649168/1‑150 (MQ=60)
                                             ACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGNGATCATCATNATCTGGTCNCAGTCATCTACCTGTACNGCGCCA                                                                                                         >  SRR19973074.285231/1‑150 (MQ=60)
                                               TACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAAC                                                                                                       >  SRR19973074.1231836/1‑150 (MQ=60)
                                                 CGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAA                                                                                                     >  SRR19973074.4046158/1‑150 (MQ=60)
                                                  GTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAAC                                                                                                    >  SRR19973074.2739216/1‑150 (MQ=60)
                                                    TTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTA                                                                                                  >  SRR19973074.3425960/1‑150 (MQ=60)
                                                     TTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAA                                                                                                 <  SRR19973074.799639/150‑1 (MQ=60)
                                                       CATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAAC                                                                                               >  SRR19973074.2762542/1‑150 (MQ=60)
                                                       CATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAAC                                                                                               >  SRR19973074.1075848/1‑150 (MQ=60)
                                                        ATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACC                                                                                              <  SRR19973074.112119/150‑1 (MQ=60)
                                                          CTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGT                                                                                            <  SRR19973074.3345180/150‑1 (MQ=60)
                                                          CTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGT                                                                                            >  SRR19973074.3958436/1‑150 (MQ=60)
                                                          CTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGT                                                                                            >  SRR19973074.3958667/1‑150 (MQ=60)
                                                             CCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTAC                                                                                         <  SRR19973074.4104437/150‑1 (MQ=60)
                                                               GCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGT                                                                                       >  SRR19973074.3116417/1‑150 (MQ=60)
                                                                CAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTT                                                                                      >  SRR19973074.1207435/1‑150 (MQ=60)
                                                                CAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTT                                                                                      <  SRR19973074.3242049/150‑1 (MQ=60)
                                                                  GTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCG                                                                                    <  SRR19973074.4099691/150‑1 (MQ=60)
                                                                    ACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGT                                                                                  >  SRR19973074.23464/1‑150 (MQ=60)
                                                                    ACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGT                                                                                  >  SRR19973074.3502669/1‑150 (MQ=60)
                                                                    ACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGT                                                                                  >  SRR19973074.1016759/1‑150 (MQ=60)
                                                                    ACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGT                                                                                  >  SRR19973074.1016763/1‑150 (MQ=60)
                                                                      GGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAA                                                                                <  SRR19973074.1158858/150‑1 (MQ=60)
                                                                       GATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAAC                                                                               >  SRR19973074.72823/1‑150 (MQ=60)
                                                                       GATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAAC                                                                               <  SRR19973074.2700646/150‑1 (MQ=60)
                                                                       GATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAAC                                                                               <  SRR19973074.2701316/150‑1 (MQ=60)
                                                                       GATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAAC                                                                               <  SRR19973074.3125896/150‑1 (MQ=60)
                                                                        ATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACC                                                                              >  SRR19973074.3578310/1‑150 (MQ=60)
                                                                        ATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACC                                                                              <  SRR19973074.3306494/150‑1 (MQ=60)
                                                                        ATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACC                                                                              >  SRR19973074.3578273/1‑150 (MQ=60)
                                                                        ATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACC                                                                              >  SRR19973074.228808/1‑150 (MQ=60)
                                                                          GAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTT                                                                            <  SRR19973074.2081238/150‑1 (MQ=60)
                                                                           AGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTG                                                                           <  SRR19973074.2126126/150‑1 (MQ=60)
                                                                           AGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTG                                                                           <  SRR19973074.2126269/150‑1 (MQ=60)
                                                                             GATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGAT                                                                         <  SRR19973074.3380490/150‑1 (MQ=60)
                                                                              ATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGGACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATG                                                                        <  SRR19973074.1499868/150‑1 (MQ=60)
                                                                               TCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGG                                                                       >  SRR19973074.1625781/1‑150 (MQ=60)
                                                                                CACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGA                                                                      >  SRR19973074.4172888/1‑150 (MQ=60)
                                                                                   GCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGAT                                                                   <  SRR19973074.1095247/150‑1 (MQ=60)
                                                                                   GCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGAT                                                                   <  SRR19973074.2132137/150‑1 (MQ=60)
                                                                                     CCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAA                                                                 <  SRR19973074.2877497/150‑1 (MQ=60)
                                                                                      CTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAAC                                                                >  SRR19973074.3661104/1‑150 (MQ=60)
                                                                                       TGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACC                                                               >  SRR19973074.3768234/1‑150 (MQ=60)
                                                                                       TGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACC                                                               >  SRR19973074.1650241/1‑150 (MQ=60)
                                                                                        GGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCC                                                              >  SRR19973074.1969645/1‑150 (MQ=60)
                                                                                        GGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCC                                                              <  SRR19973074.1578035/150‑1 (MQ=60)
                                                                                        GGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCC                                                              >  SRR19973074.1646860/1‑150 (MQ=60)
                                                                                        GGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTACGGTAACCTTGATGGAGATAACCC                                                              >  SRR19973074.1081143/1‑150 (MQ=60)
                                                                                         GGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCC                                                             >  SRR19973074.17548/1‑150 (MQ=60)
                                                                                         GGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCC                                                             >  SRR19973074.2420651/1‑150 (MQ=60)
                                                                                          GAGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCT                                                            <  SRR19973074.2139428/150‑1 (MQ=60)
                                                                                           TGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTT                                                           <  SRR19973074.1368876/150‑1 (MQ=60)
                                                                                           TGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTT                                                           <  SRR19973074.328368/150‑1 (MQ=60)
                                                                                           TGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTT                                                           <  SRR19973074.328561/150‑1 (MQ=60)
                                                                                           TGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTT                                                           <  SRR19973074.3604601/150‑1 (MQ=60)
                                                                                           TGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTT                                                           <  SRR19973074.3604595/150‑1 (MQ=60)
                                                                                           TGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTT                                                           <  SRR19973074.3604525/150‑1 (MQ=60)
                                                                                           TGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTT                                                           >  SRR19973074.2548376/1‑150 (MQ=60)
                                                                                             TTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTC                                                         <  SRR19973074.1331906/150‑1 (MQ=60)
                                                                                               ACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGT                                                       >  SRR19973074.2682059/1‑150 (MQ=60)
                                                                                                CGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTC                                                      <  SRR19973074.1877476/150‑1 (MQ=60)
                                                                                                 GGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCG                                                     <  SRR19973074.2615601/150‑1 (MQ=60)
                                                                                                    CCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCAC                                                  >  SRR19973074.3969641/1‑150 (MQ=60)
                                                                                                     CACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACG                                                 >  SRR19973074.3797174/1‑150 (MQ=60)
                                                                                                     CACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACG                                                 <  SRR19973074.2883480/150‑1 (MQ=60)
                                                                                                       CGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCG                                               <  SRR19973074.129062/150‑1 (MQ=60)
                                                                                                       CGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCG                                               >  SRR19973074.3960892/1‑150 (MQ=60)
                                                                                                           GCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTT                                           <  SRR19973074.210715/150‑1 (MQ=60)
                                                                                                           GCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTT                                           >  SRR19973074.3804204/1‑150 (MQ=60)
                                                                                                            CTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTG                                          >  SRR19973074.2673116/1‑150 (MQ=60)
                                                                                                            CTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTG                                          >  SRR19973074.2673135/1‑150 (MQ=60)
                                                                                                            CTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTG                                          >  SRR19973074.2673185/1‑150 (MQ=60)
                                                                                                             TGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGG                                         <  SRR19973074.3514864/150‑1 (MQ=60)
                                                                                                               ATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTT                                       >  SRR19973074.1799329/1‑150 (MQ=60)
                                                                                                                    CGAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTGGGGTA                                  >  SRR19973074.1757451/1‑150 (MQ=60)
                                                                                                                     GAAACGCGAGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTAT                                 <  SRR19973074.1434461/150‑1 (MQ=60)
                                                                                                                             AGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCAC                         >  SRR19973074.202488/1‑150 (MQ=60)
                                                                                                                             AGTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAACCCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCAC                         <  SRR19973074.568488/150‑1 (MQ=60)
                                                                                                                              GTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACT                        <  SRR19973074.1329368/150‑1 (MQ=60)
                                                                                                                              GTACGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACT                        <  SRR19973074.1329360/150‑1 (MQ=60)
                                                                                                                                 CGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCG                     <  SRR19973074.3830480/150‑1 (MQ=60)
                                                                                                                                 CGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCG                     <  SRR19973074.2890530/150‑1 (MQ=60)
                                                                                                                                 CGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCG                     >  SRR19973074.3666858/1‑150 (MQ=60)
                                                                                                                                 CGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGGTGGGTATTCCGCCACTGCG                     >  SRR19973074.4108763/1‑150 (MQ=60)
                                                                                                                                 CGTACCAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACGTGGTTGGGTATTCCGCCACTGCG                     >  SRR19973074.3666921/1‑150 (MQ=60)
                                                                                                                                      CAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACG                >  SRR19973074.1322296/1‑150 (MQ=60)
                                                                                                                                      CAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACG                >  SRR19973074.2329465/1‑150 (MQ=60)
                                                                                                                                      CAGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACG                >  SRR19973074.3286777/1‑150 (MQ=60)
                                                                                                                                       AGCGTACCTGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGT               >  SRR19973074.221809/1‑150 (MQ=60)
                                                                                                                                       AGCGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGT               >  SRR19973074.2677800/1‑150 (MQ=60)
                                                                                                                                         CGTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTT             <  SRR19973074.1889363/150‑1 (MQ=60)
                                                                                                                                          GTACCGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTA            >  SRR19973074.564929/1‑150 (MQ=60)
                                                                                                                                              CGGCATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGGTGGGTATTCCGCCACTGCGGTACGTTTACCGT        >  SRR19973074.441274/1‑150 (MQ=60)
                                                                                                                                                  ATAGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACC    >  SRR19973074.2174001/1‑150 (MQ=60)
                                                                                                                                                    AGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACCGT  >  SRR19973074.1100751/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
TCCAGATCTTCCTCGTCAACCGGTTCAGCAACACGTTGCAGACCCACTACGTTTTCATCTTCCGCAGTACGGATGAGGATCACGCCCTGGGTGTTACGGCCCACGATGCTGATTTCCGAAACGCGAGTACGTACCAGCGTACCGGCATCGGTGATCATCATGATCTGGTCGCAGTCATCTACCTGTACCGCGCCAACAACTAAACCGTTACGTTCGGTAACCTTGATGGAGATAACCCCTTTCGTCGCACGCGACTTGGTTGGGTATTCCGCCACTGCGGTACGTTTACCGTAACCGT  >  CP009273/2330354‑2330651

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑