Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,454,297 T→G 100% E39D (GAA→GAC)  yfcZ ← UPF0381 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,454,2970TG99.6% 856.4 / ‑5.2 209E39D (GAA→GAC) yfcZUPF0381 family protein
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base G (101/107);  total (102/107)
Fisher's exact test for biased strand distribution p-value = 4.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 213 total aligned reads displayed.

CAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCTGTATGTGCACCCA  >  CP009273/2454153‑2454440
                                                                                                                                                |                                                                                                                                               
cAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCt                                                                                                                                              <  1:935700/148‑1 (MQ=255)
cAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCt                                                                                                                                              >  2:3391804/1‑148 (MQ=255)
cAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCt                                                                                                                                              >  1:2899391/1‑148 (MQ=255)
   cATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCtctgcgtctgcg                                                                                                                                           >  1:2419203/1‑148 (MQ=255)
   cATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCtctgcgtctgcg                                                                                                                                           >  1:2729515/1‑148 (MQ=255)
   cATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCtctgcgtctgcg                                                                                                                                           <  1:1767169/148‑1 (MQ=255)
     tttCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGcgcg                                                                                                                                         >  2:62109/1‑148 (MQ=255)
     tttCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGcgcg                                                                                                                                         >  2:2649120/1‑148 (MQ=255)
      ttCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCgg                                                                                                                                        >  1:2275442/1‑148 (MQ=255)
      ttCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCgg                                                                                                                                        <  1:141144/148‑1 (MQ=255)
      ttCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCgg                                                                                                                                        >  2:1318281/1‑148 (MQ=255)
         aGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGttt                                                                                                                                     >  2:407288/1‑148 (MQ=255)
           cTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGc                                                                                                                                   >  1:2549643/1‑148 (MQ=255)
           cTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGc                                                                                                                                   >  1:3021710/1‑148 (MQ=255)
               gCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAac                                                                                                                               <  1:2323850/148‑1 (MQ=255)
               gCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAac                                                                                                                               <  1:2817436/148‑1 (MQ=255)
                  ggCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACAcg                                                                                                                            <  1:2198454/148‑1 (MQ=255)
                  ggCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACAcg                                                                                                                            <  2:1299085/148‑1 (MQ=255)
                    cAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACAcgcg                                                                                                                          >  1:1258754/1‑148 (MQ=255)
                     aaaaGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCgg                                                                                                                         <  1:3244436/148‑1 (MQ=255)
                         gTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGt                                                                                                                     >  1:3528073/1‑148 (MQ=255)
                         gTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGt                                                                                                                     <  1:514830/148‑1 (MQ=255)
                          tGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTa                                                                                                                    >  1:542104/1‑148 (MQ=255)
                            aaaTCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAg                                                                                                                  <  2:1677839/148‑1 (MQ=255)
                            aaaTCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAg                                                                                                                  >  2:1111646/1‑148 (MQ=255)
                            aaaTCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAg                                                                                                                  <  1:1126895/148‑1 (MQ=255)
                             aaTCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGa                                                                                                                 >  1:2010862/1‑148 (MQ=255)
                                    atCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTg                                                                                                          >  1:2643204/1‑148 (MQ=255)
                                     tCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGc                                                                                                         >  1:2248661/1‑148 (MQ=255)
                                     tCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGc                                                                                                         >  2:1757816/1‑148 (MQ=255)
                                      ccAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCa                                                                                                        <  1:1483278/148‑1 (MQ=255)
                                          gcgcACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCg                                                                                                    >  2:2975159/1‑148 (MQ=255)
                                             cacaCCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAg                                                                                                 >  2:1238449/1‑148 (MQ=255)
                                                  cGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTc                                                                                            >  1:3085267/1‑148 (MQ=255)
                                                   gTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTcc                                                                                           <  2:2445133/148‑1 (MQ=255)
                                                   gTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGAGTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTcc                                                                                           <  2:2087538/148‑1 (MQ=255)
                                                   gTCAGACTCTTCAGTGAAAGTCGGGGTGATTGTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTcc                                                                                           <  2:2863551/148‑1 (MQ=255)
                                                    tCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCa                                                                                          <  2:1078453/148‑1 (MQ=255)
                                                       gACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATaa                                                                                       <  1:1295876/148‑1 (MQ=255)
                                                        aCTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAAt                                                                                      >  1:2877762/1‑148 (MQ=255)
                                                        aCTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAAt                                                                                      >  1:1472953/1‑148 (MQ=255)
                                                         ctctTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATg                                                                                     <  2:1467032/148‑1 (MQ=255)
                                                           ctTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGt                                                                                   <  2:2006617/148‑1 (MQ=255)
                                                            ttCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTg                                                                                  <  1:2032912/148‑1 (MQ=255)
                                                             tCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGc                                                                                 <  2:2533156/148‑1 (MQ=255)
                                                              cAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGcc                                                                                <  1:1916176/148‑1 (MQ=255)
                                                               aGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCa                                                                               <  1:2437863/148‑1 (MQ=255)
                                                                 tGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAAc                                                                             >  1:304552/1‑148 (MQ=255)
                                                                 tGAAAGTCGGGGTGATTGTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAAc                                                                             <  2:2851429/148‑1 (MQ=255)
                                                                   aaaGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACAt                                                                           <  2:3417190/148‑1 (MQ=255)
                                                                          gggTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATAca                                                                    >  1:2365070/1‑148 (MQ=255)
                                                                           ggTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATAcag                                                                   <  1:591096/148‑1 (MQ=255)
                                                                                 tttGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCaa                                                             >  2:1975628/1‑148 (MQ=255)
                                                                                 tttGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCaa                                                             >  2:1164153/1‑148 (MQ=255)
                                                                                  ttGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCaaa                                                            <  2:1818012/148‑1 (MQ=255)
                                                                                      aaGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCgg                                                        <  1:2529302/148‑1 (MQ=255)
                                                                                       aGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCggg                                                       <  2:1730864/148‑1 (MQ=255)
                                                                                        ggTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGACGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCgggg                                                      >  2:965671/1‑148 (MQ=255)
                                                                                          ttCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGtt                                                    <  2:2010819/148‑1 (MQ=255)
                                                                                          ttCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGtt                                                    >  1:2626467/1‑148 (MQ=255)
                                                                                               agtCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTtcatc                                               <  2:2173606/146‑1 (MQ=255)
                                                                                               aTTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTtcatc                                               >  1:2174398/1‑148 (MQ=255)
                                                                                               aTTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTtcatc                                               >  2:2259439/1‑148 (MQ=255)
                                                                                               aTTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTtcatc                                               <  2:2173653/148‑1 (MQ=255)
                                                                                                ttCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTtcatca                                              >  2:3288070/1‑148 (MQ=255)
                                                                                                 tCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAg                                             <  1:1484193/148‑1 (MQ=255)
                                                                                                   cACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCa                                           <  2:3225421/148‑1 (MQ=255)
                                                                                                   cACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCa                                           <  1:907610/148‑1 (MQ=255)
                                                                                                   cACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCa                                           <  1:2685642/148‑1 (MQ=255)
                                                                                                    aCGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCAc                                          >  2:466535/1‑148 (MQ=255)
                                                                                                    aCGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCAc                                          <  2:817202/148‑1 (MQ=255)
                                                                                                        tACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACa                                      <  2:1362012/148‑1 (MQ=255)
                                                                                                         aCGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACAt                                     <  2:3194904/148‑1 (MQ=255)
                                                                                                         aCGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACAt                                     >  1:2547125/1‑148 (MQ=255)
                                                                                                           gggCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACAttt                                   <  1:1017711/148‑1 (MQ=255)
                                                                                                           gggCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACAttt                                   <  2:523655/148‑1 (MQ=255)
                                                                                                            ggCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTa                                  >  2:192946/1‑148 (MQ=255)
                                                                                                            ggCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTa                                  <  1:104507/148‑1 (MQ=255)
                                                                                                            ggCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTa                                  >  2:2225400/1‑148 (MQ=255)
                                                                                                            ggCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTa                                  <  1:104059/148‑1 (MQ=255)
                                                                                                            ggCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTa                                  <  1:1588556/148‑1 (MQ=255)
                                                                                                             gCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTAc                                 >  1:3423517/1‑148 (MQ=255)
                                                                                                                ttttCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCa                              >  2:578121/1‑148 (MQ=255)
                                                                                                                   tcagtcagCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCg                           <  2:2275442/148‑1 (MQ=255)
                                                                                                                   tcagtcagCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCg                           <  1:640967/148‑1 (MQ=255)
                                                                                                                      gtcagCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTg                        >  1:1834564/1‑148 (MQ=255)
                                                                                                                      gtcagCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTg                        >  1:1193182/1‑148 (MQ=255)
                                                                                                                       tcagCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGa                       <  2:2019737/148‑1 (MQ=255)
                                                                                                                        cagCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGAt                      <  1:2982807/148‑1 (MQ=255)
                                                                                                                          gcgcCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGAttt                    >  2:3082167/1‑148 (MQ=255)
                                                                                                                          gcgcCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGAttt                    <  1:1085447/148‑1 (MQ=255)
                                                                                                                             ccgccAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCct                 >  1:772312/1‑148 (MQ=255)
                                                                                                                              cgccAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCctc                <  1:332791/148‑1 (MQ=255)
                                                                                                                               gccAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCctct               <  1:1851927/148‑1 (MQ=255)
                                                                                                                                ccAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCTg              >  1:1822986/1‑148 (MQ=255)
                                                                                                                                 cAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCTGt             <  2:2566645/148‑1 (MQ=255)
                                                                                                                                 cAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCTGt             >  1:3159310/1‑148 (MQ=255)
                                                                                                                                    cGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCTGTAtg          >  1:3081769/1‑148 (MQ=255)
                                                                                                                                      tttGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCTGTAtgtg        <  2:1717226/148‑1 (MQ=255)
                                                                                                                                          ctctGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCTGTATGTGCAcc    <  1:2813341/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                               
CAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCTGTATGTGCACCCA  >  CP009273/2454153‑2454440

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCTGTATGTGCACCCAAGGTG  >  CP009273/2454148‑2454445
                                                                                                                                                     |                                                                                                                                                    
AAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCG                                                                                                                                                      >  SRR19973067.2583404/1‑150 (MQ=60)
   ATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCT                                                                                                                                                   >  SRR19973067.2917888/1‑150 (MQ=60)
   ATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCT                                                                                                                                                   >  SRR19973067.1323235/1‑150 (MQ=60)
     CAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGC                                                                                                                                                 <  SRR19973067.942052/150‑1 (MQ=60)
      AGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCG                                                                                                                                                >  SRR19973067.2747127/1‑150 (MQ=60)
      AGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCG                                                                                                                                                >  SRR19973067.2436320/1‑150 (MQ=60)
      AGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCG                                                                                                                                                >  SRR19973067.2435055/1‑150 (MQ=60)
        CATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCG                                                                                                                                              <  SRR19973067.1779173/150‑1 (MQ=60)
         ATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGG                                                                                                                                             >  SRR19973067.2290501/1‑150 (MQ=60)
         ATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGG                                                                                                                                             >  SRR19973067.2290468/1‑150 (MQ=60)
           TTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTT                                                                                                                                           <  SRR19973067.142391/150‑1 (MQ=60)
              AGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGC                                                                                                                                        >  SRR19973067.2566322/1‑150 (MQ=60)
              AGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGC                                                                                                                                        >  SRR19973067.3040950/1‑150 (MQ=60)
                  TCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAAC                                                                                                                                    <  SRR19973067.947463/150‑1 (MQ=60)
                  TCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAAC                                                                                                                                    <  SRR19973067.1475361/150‑1 (MQ=60)
                   CGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACA                                                                                                                                   <  SRR19973067.2357471/150‑1 (MQ=60)
                    GCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACAC                                                                                                                                  <  SRR19973067.2835579/150‑1 (MQ=60)
                    GCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACAC                                                                                                                                  <  SRR19973067.2339119/150‑1 (MQ=60)
                    GCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACAC                                                                                                                                  <  SRR19973067.539539/150‑1 (MQ=60)
                       GGCAAAGGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCG                                                                                                                               >  SRR19973067.3048868/1‑150 (MQ=60)
                       GGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCG                                                                                                                               <  SRR19973067.2213124/150‑1 (MQ=60)
                       GGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCG                                                                                                                               >  SRR19973067.1267279/1‑150 (MQ=60)
                          AAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCT                                                                                                                            <  SRR19973067.3264760/150‑1 (MQ=60)
                            AAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGT                                                                                                                          >  SRR19973067.3550383/1‑150 (MQ=60)
                            AAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGT                                                                                                                          >  SRR19973067.1778906/1‑150 (MQ=60)
                             AGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTA                                                                                                                         >  SRR19973067.545707/1‑150 (MQ=60)
                              GTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAA                                                                                                                        <  SRR19973067.518326/150‑1 (MQ=60)
                                GAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGA                                                                                                                      >  SRR19973067.2199587/1‑150 (MQ=60)
                                GAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGA                                                                                                                      >  SRR19973067.2024394/1‑150 (MQ=60)
                                GAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGA                                                                                                                      >  SRR19973067.2024351/1‑150 (MQ=60)
                                 AAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGAC                                                                                                                     <  SRR19973067.1134505/150‑1 (MQ=60)
                                 AAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGAC                                                                                                                     >  SRR19973067.2103038/1‑150 (MQ=60)
                                   ATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGC                                                                                                                   >  SRR19973067.1118600/1‑150 (MQ=60)
                                     CGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGG                                                                                                                 >  SRR19973067.3245682/1‑150 (MQ=60)
                                     CGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGG                                                                                                                 >  SRR19973067.2926073/1‑150 (MQ=60)
                                      GATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGT                                                                                                                >  SRR19973067.41508/1‑150 (MQ=60)
                                       ATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTG                                                                                                               >  SRR19973067.2660277/1‑150 (MQ=60)
                                        TATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGC                                                                                                              >  SRR19973067.2263598/1‑150 (MQ=60)
                                        TATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGC                                                                                                              >  SRR19973067.2263515/1‑150 (MQ=60)
                                           CCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGT                                                                                                           <  SRR19973067.1493113/150‑1 (MQ=60)
                                           CCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGT                                                                                                           <  SRR19973067.1493569/150‑1 (MQ=60)
                                                CGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAG                                                                                                      <  SRR19973067.27617/150‑1 (MQ=60)
                                                CGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAG                                                                                                      <  SRR19973067.1075382/150‑1 (MQ=60)
                                                  CACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTT                                                                                                    <  SRR19973067.1298312/150‑1 (MQ=60)
                                                   ACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTG                                                                                                   >  SRR19973067.1490160/1‑150 (MQ=60)
                                                   ACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTG                                                                                                   >  SRR19973067.3269966/1‑150 (MQ=60)
                                                    CACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGT                                                                                                  >  SRR19973067.1546668/1‑150 (MQ=60)
                                                     ACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTC                                                                                                 >  SRR19973067.3104897/1‑150 (MQ=60)
                                                          CAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAA                                                                                            >  SRR19973067.2626152/1‑150 (MQ=60)
                                                           AGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAAT                                                                                           <  SRR19973067.1922350/150‑1 (MQ=60)
                                                           AGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAAT                                                                                           <  SRR19973067.2868003/150‑1 (MQ=60)
                                                           AGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAAT                                                                                           >  SRR19973067.2896171/1‑150 (MQ=60)
                                                           AGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAAT                                                                                           <  SRR19973067.114005/150‑1 (MQ=60)
                                                           AGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAAT                                                                                           <  SRR19973067.2952054/150‑1 (MQ=60)
                                                           AGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAAT                                                                                           >  SRR19973067.1482759/1‑150 (MQ=60)
                                                            GACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATG                                                                                          <  SRR19973067.1304722/150‑1 (MQ=60)
                                                                 TTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCC                                                                                     <  SRR19973067.2046533/150‑1 (MQ=60)
                                                                   CAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAA                                                                                   <  SRR19973067.1929255/150‑1 (MQ=60)
                                                                    AGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAAC                                                                                  >  SRR19973067.306514/1‑150 (MQ=60)
                                                                    AGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAAC                                                                                  <  SRR19973067.2453872/150‑1 (MQ=60)
                                                                      TGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACAT                                                                                <  SRR19973067.2917669/150‑1 (MQ=60)
                                                                          AGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCAT                                                                            >  SRR19973067.3034377/1‑150 (MQ=60)
                                                                             CGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACA                                                                         >  SRR19973067.2380520/1‑150 (MQ=60)
                                                                             CGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACA                                                                         >  SRR19973067.2380474/1‑150 (MQ=60)
                                                                                GGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCA                                                                      <  SRR19973067.594858/150‑1 (MQ=60)
                                                                                  TGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGC                                                                    >  SRR19973067.2752610/1‑150 (MQ=60)
                                                                                  TGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGC                                                                    >  SRR19973067.2557458/1‑150 (MQ=60)
                                                                                   GATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCA                                                                   <  SRR19973067.859099/150‑1 (MQ=60)
                                                                                           AAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGG                                                           >  SRR19973067.2460528/1‑150 (MQ=60)
                                                                                           AAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGG                                                           <  SRR19973067.2545874/150‑1 (MQ=60)
                                                                                             GGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTT                                                         <  SRR19973067.3413381/150‑1 (MQ=60)
                                                                                             GGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTT                                                         >  SRR19973067.2643446/1‑150 (MQ=60)
                                                                                             GGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTT                                                         >  SRR19973067.2643490/1‑150 (MQ=60)
                                                                                              GTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTT                                                        <  SRR19973067.2649631/150‑1 (MQ=60)
                                                                                              GTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTT                                                        >  SRR19973067.568847/1‑150 (MQ=60)
                                                                                               TTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTC                                                       <  SRR19973067.1850609/150‑1 (MQ=60)
                                                                                                  AGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATC                                                    <  SRR19973067.1320014/150‑1 (MQ=60)
                                                                                                  AGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATC                                                    >  SRR19973067.2305325/1‑150 (MQ=60)
                                                                                                  AGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATC                                                    >  SRR19973067.2304854/1‑150 (MQ=60)
                                                                                                  AGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATC                                                    >  SRR19973067.2188941/1‑150 (MQ=60)
                                                                                                      TCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCA                                                <  SRR19973067.1494033/150‑1 (MQ=60)
                                                                                                       CCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCAC                                               >  SRR19973067.1049221/1‑150 (MQ=60)
                                                                                                       CCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCAC                                               >  SRR19973067.2834287/1‑150 (MQ=60)
                                                                                                        CACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACT                                              <  SRR19973067.1841234/150‑1 (MQ=60)
                                                                                                        CACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACT                                              <  SRR19973067.913620/150‑1 (MQ=60)
                                                                                                        CACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACT                                              <  SRR19973067.913902/150‑1 (MQ=60)
                                                                                                        CACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACT                                              <  SRR19973067.2702871/150‑1 (MQ=60)
                                                                                                          CGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACAAC                                            >  SRR19973067.2026437/1‑150 (MQ=60)
                                                                                                            CTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACAT                                          >  SRR19973067.2563913/1‑150 (MQ=60)
                                                                                                            CTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACAT                                          >  SRR19973067.2563788/1‑150 (MQ=60)
                                                                                                             TACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATT                                         >  SRR19973067.717845/1‑150 (MQ=60)
                                                                                                                GGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTAC                                      >  SRR19973067.3445301/1‑150 (MQ=60)
                                                                                                                GGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTAC                                      <  SRR19973067.1024718/150‑1 (MQ=60)
                                                                                                                GGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTAC                                      <  SRR19973067.1024695/150‑1 (MQ=60)
                                                                                                                 GGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACT                                     <  SRR19973067.2666209/150‑1 (MQ=60)
                                                                                                                 GGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACT                                     >  SRR19973067.3102402/1‑150 (MQ=60)
                                                                                                                 GGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACT                                     <  SRR19973067.105547/150‑1 (MQ=60)
                                                                                                                 GGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACT                                     >  SRR19973067.3105128/1‑150 (MQ=60)
                                                                                                                 GGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACT                                     <  SRR19973067.105096/150‑1 (MQ=60)
                                                                                                                 GGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACT                                     <  SRR19973067.1599442/150‑1 (MQ=60)
                                                                                                                        TCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTT                              <  SRR19973067.645282/150‑1 (MQ=60)
                                                                                                                         CAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTG                             >  SRR19973067.1847056/1‑150 (MQ=60)
                                                                                                                         CAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTG                             >  SRR19973067.1204702/1‑150 (MQ=60)
                                                                                                                         CAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTG                             >  SRR19973067.1201014/1‑150 (MQ=60)
                                                                                                                           GTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGAT                           <  SRR19973067.62861/150‑1 (MQ=60)
                                                                                                                             CAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTT                         >  SRR19973067.827909/1‑150 (MQ=60)
                                                                                                                             CAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTT                         <  SRR19973067.3001655/150‑1 (MQ=60)
                                                                                                                               GCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCC                       <  SRR19973067.1092872/150‑1 (MQ=60)
                                                                                                                                CGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCT                      >  SRR19973067.777661/1‑150 (MQ=60)
                                                                                                                                   CGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCTG                   <  SRR19973067.335064/150‑1 (MQ=60)
                                                                                                                                   CGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCTG                   >  SRR19973067.3403686/1‑150 (MQ=60)
                                                                                                                                   CGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCTG                   >  SRR19973067.1835341/1‑150 (MQ=60)
                                                                                                                                   CGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCTG                   >  SRR19973067.1835350/1‑150 (MQ=60)
                                                                                                                                    GCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCTGT                  <  SRR19973067.1864549/150‑1 (MQ=60)
                                                                                                                                    GCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCTGT                  <  SRR19973067.1864231/150‑1 (MQ=60)
                                                                                                                                    GCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCTGT                  >  SRR19973067.3469415/1‑150 (MQ=60)
                                                                                                                                    GCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCTGT                  >  SRR19973067.3179328/1‑150 (MQ=60)
                                                                                                                                       ATCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGAAAACCGGGGTTTCATCAGCACTACATTTACTCAACGTTGATTTCCTCTGTATG               >  SRR19973067.3101250/1‑150 (MQ=60)
                                                                                                                                       AGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCTGTATG               >  SRR19973067.3101379/1‑150 (MQ=60)
                                                                                                                                               CTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCTGTATGTGCACCCA       <  SRR19973067.2831466/150‑1 (MQ=60)
                                                                                                                                                    CGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCTGTATGTGCACCCAAGGTG  >  SRR19973067.1875441/1‑150 (MQ=60)
                                                                                                                                                    CGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCTGTATGTGCACCCAAGGTG  >  SRR19973067.3306269/1‑150 (MQ=60)
                                                                                                                                                    CGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCTGTATGTGCACCCAAGGTG  >  SRR19973067.227255/1‑150 (MQ=60)
                                                                                                                                                    CGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCTGTATGTGCACCCAAGGTG  >  SRR19973067.1528862/1‑150 (MQ=60)
                                                                                                                                                    CGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCTGTATGTGCACCCAAGGTG  >  SRR19973067.1528880/1‑150 (MQ=60)
                                                                                                                                                    CGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCTGTATGTGCACCCAAGGTG  >  SRR19973067.231308/1‑150 (MQ=60)
                                                                                                                                                    CGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAAAGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCTTTGATTTCCTCTGTATGTGCACCCAAGGTG  >  SRR19973067.752971/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
AAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCTGTATGTGCACCCAAGGTG  >  CP009273/2454148‑2454445

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑