Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F204 I0 R1 1155 255.8 8356456 95.7% 7997128 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,454,297 T→G 100% E39D (GAA→GAC)  yfcZ ← UPF0381 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,454,2970TG99.6% 952.9 / ‑5.0 231E39D (GAA→GAC) yfcZUPF0381 family protein
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base G (113/117);  total (113/118)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 232 total aligned reads displayed.

AATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCTGTATGTGCACCCAAGG  >  CP009273/2454150‑2454443
                                                                                                                                                   |                                                                                                                                                  
aaTCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTc                                                                                                                                                  >  2:2671859/1‑150 (MQ=255)
 aTCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCt                                                                                                                                                 <  2:639940/150‑1 (MQ=255)
  tCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTg                                                                                                                                                <  1:4157494/150‑1 (MQ=255)
  tCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTg                                                                                                                                                <  1:3025527/150‑1 (MQ=255)
       aTTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCgg                                                                                                                                           >  1:770031/1‑150 (MQ=255)
        tttCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGt                                                                                                                                          >  2:2954224/1‑150 (MQ=255)
         ttCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGtt                                                                                                                                         >  2:2538842/1‑150 (MQ=255)
            aGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGc                                                                                                                                      >  1:357563/1‑150 (MQ=255)
            aGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGc                                                                                                                                      >  2:404924/1‑150 (MQ=255)
                tCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAac                                                                                                                                  <  1:1211732/150‑1 (MQ=255)
                  gCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCTTCTGCGCGGTTTGCAAacac                                                                                                                                <  1:1854999/150‑1 (MQ=255)
                    aGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACAcgc                                                                                                                              <  1:2817941/150‑1 (MQ=255)
                     ggCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACAcgcg                                                                                                                             <  2:3158551/150‑1 (MQ=255)
                     ggCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACAcgcg                                                                                                                             <  2:3885268/150‑1 (MQ=255)
                     ggCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACAcgcg                                                                                                                             <  2:203798/150‑1 (MQ=255)
                       cAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGc                                                                                                                           <  1:1212411/150‑1 (MQ=255)
                       cAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGc                                                                                                                           >  2:1343877/1‑150 (MQ=255)
                        aaaaGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCt                                                                                                                          >  1:994851/1‑150 (MQ=255)
                         aaaGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTg                                                                                                                         >  2:2244488/1‑150 (MQ=255)
                          aaGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGt                                                                                                                        >  1:1874765/1‑150 (MQ=255)
                           aGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTa                                                                                                                       <  2:1954126/150‑1 (MQ=255)
                            gTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTaa                                                                                                                      <  2:3229365/150‑1 (MQ=255)
                             tGAAATCGATATCCAGGCGCACACCGTCAGACTCGTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAg                                                                                                                     <  2:2630834/150‑1 (MQ=255)
                               aaaTCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGAc                                                                                                                   >  2:1406316/1‑150 (MQ=255)
                               aaaTCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGAc                                                                                                                   >  2:2872663/1‑150 (MQ=255)
                               aaaTCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGAc                                                                                                                   >  2:452571/1‑150 (MQ=255)
                                 aTCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGAcgc                                                                                                                 >  1:1495972/1‑150 (MQ=255)
                                    gATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGt                                                                                                              <  1:2215565/150‑1 (MQ=255)
                                    gATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGt                                                                                                              >  2:1011833/1‑150 (MQ=255)
                                    gATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGt                                                                                                              <  1:1990375/150‑1 (MQ=255)
                                    gATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGt                                                                                                              <  1:1769807/150‑1 (MQ=255)
                                     atatCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTg                                                                                                             >  2:207609/1‑150 (MQ=255)
                                      tatCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGc                                                                                                            <  1:717228/150‑1 (MQ=255)
                                          cAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTc                                                                                                        <  1:2229811/150‑1 (MQ=255)
                                           aGGCGCACACCGTCAGACTCTTCAGTGAAANTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGGTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCg                                                                                                       >  1:3854610/1‑150 (MQ=255)
                                           aGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCg                                                                                                       <  1:3328868/150‑1 (MQ=255)
                                           aGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCg                                                                                                       >  1:2404655/1‑150 (MQ=255)
                                             gcgcACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGa                                                                                                     <  2:365156/150‑1 (MQ=255)
                                               gcACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGt                                                                                                   >  1:3054160/1‑150 (MQ=255)
                                                cacaCCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGtt                                                                                                  <  1:696729/150‑1 (MQ=255)
                                                cacaCCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGtt                                                                                                  >  2:3449510/1‑150 (MQ=255)
                                                      gTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCAt                                                                                            <  1:136408/150‑1 (MQ=255)
                                                        cAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATaa                                                                                          >  2:3006454/1‑150 (MQ=255)
                                                            ctctTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGt                                                                                      <  2:3207593/150‑1 (MQ=255)
                                                            ctctTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGt                                                                                      >  1:58657/1‑150 (MQ=255)
                                                                tCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCa                                                                                  >  2:730882/1‑150 (MQ=255)
                                                                 cAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCaa                                                                                 <  1:2529621/150‑1 (MQ=255)
                                                                     gAAACTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATc                                                                             >  1:257623/1‑150 (MQ=255)
                                                                        aGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCAt                                                                          <  2:496409/150‑1 (MQ=255)
                                                                        aGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCAt                                                                          <  1:784939/150‑1 (MQ=255)
                                                                        aGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCGACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCAt                                                                          <  2:1632598/150‑1 (MQ=255)
                                                                          tCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATAc                                                                        >  2:1947189/1‑150 (MQ=255)
                                                                           cGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATAca                                                                       <  1:344456/150‑1 (MQ=255)
                                                                            ggggTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATAcag                                                                      >  1:1636387/1‑150 (MQ=255)
                                                                               gTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATAcagcag                                                                   >  1:3777737/1‑150 (MQ=255)
                                                                                tGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACagcagc                                                                  <  1:1618969/150‑1 (MQ=255)
                                                                                  aTTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCaa                                                                <  1:1411274/150‑1 (MQ=255)
                                                                                      tGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCg                                                            <  2:1888641/150‑1 (MQ=255)
                                                                                       gCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCgg                                                           <  1:398289/150‑1 (MQ=255)
                                                                                           ggTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGtt                                                       <  1:3545692/150‑1 (MQ=255)
                                                                                           ggTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGtt                                                       <  2:2652507/150‑1 (MQ=255)
                                                                                            gTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGttt                                                      >  2:3801059/1‑150 (MQ=255)
                                                                                            gTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGttt                                                      <  2:167397/150‑1 (MQ=255)
                                                                                              tCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTtca                                                    >  2:2382551/1‑150 (MQ=255)
                                                                                                aGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTtcatc                                                  >  1:471723/1‑150 (MQ=255)
                                                                                                aGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTtcatc                                                  >  1:471656/1‑150 (MQ=255)
                                                                                                 gATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTtcatca                                                 <  1:2011339/150‑1 (MQ=255)
                                                                                                      cACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACt                                            >  1:2079891/1‑150 (MQ=255)
                                                                                                       aCGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGACAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTa                                           >  1:892598/1‑150 (MQ=255)
                                                                                                         gCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACa                                         >  1:190324/1‑150 (MQ=255)
                                                                                                            aCGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACAttt                                      <  1:1607147/150‑1 (MQ=255)
                                                                                                              gggCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTAc                                    <  2:2371131/150‑1 (MQ=255)
                                                                                                                  tttttCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCAt                                <  1:3410352/150‑1 (MQ=255)
                                                                                                                    tttCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCg                              >  1:3691563/1‑150 (MQ=255)
                                                                                                                      tcagtcagCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGtt                            <  2:1197954/150‑1 (MQ=255)
                                                                                                                       cagtcagCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTg                           >  1:1060224/1‑150 (MQ=255)
                                                                                                                         gtcagCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGAt                         >  2:2782304/1‑150 (MQ=255)
                                                                                                                         gtcagCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGAt                         <  1:1241529/150‑1 (MQ=255)
                                                                                                                         gtcagCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTGTCATCAGCACTACATTTACTCATCGTTGAt                         <  1:2139443/150‑1 (MQ=255)
                                                                                                                          tcagCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGAtt                        <  1:3201381/150‑1 (MQ=255)
                                                                                                                          tcagCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGAtt                        <  1:1920628/150‑1 (MQ=255)
                                                                                                                          tcagCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGAtt                        <  2:4003393/150‑1 (MQ=255)
                                                                                                                               gccgccAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCctc                   <  2:2462244/150‑1 (MQ=255)
                                                                                                                               gccgccAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCctc                   <  2:2462293/150‑1 (MQ=255)
                                                                                                                                ccgccAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCctct                  <  1:2899332/150‑1 (MQ=255)
                                                                                                                                ccgccAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCctct                  <  2:4120789/150‑1 (MQ=255)
                                                                                                                                ccgccAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCctct                  >  1:2667399/1‑150 (MQ=255)
                                                                                                                                   ccAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCTGTa               >  2:1982683/1‑150 (MQ=255)
                                                                                                                                    cAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCTGTAt              <  2:4034915/150‑1 (MQ=255)
                                                                                                                                     aGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCTTTAtg             >  1:3870159/1‑150 (MQ=255)
                                                                                                                                     aGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCTGTAtg             >  1:2679698/1‑150 (MQ=255)
                                                                                                                                      gCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCTGTAtgt            <  1:634542/150‑1 (MQ=255)
                                                                                                                                          ttGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCTGTATGTGCAc        >  1:3975795/1‑150 (MQ=255)
                                                                                                                                          ttGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCTGTATGTGCAc        >  1:3975872/1‑150 (MQ=255)
                                                                                                                                           tGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCTGTATGTGCAcc       >  2:3897805/1‑150 (MQ=255)
                                                                                                                                            gCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCTGTATGTGCAccc      <  2:3554428/150‑1 (MQ=255)
                                                                                                                                              tctgcgtctgcgCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCTGTATGTGCACCCaa    >  1:2608813/1‑150 (MQ=255)
                                                                                                                                               ctGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCTGTATGTGCACCCAAg   >  2:1293878/1‑150 (MQ=255)
                                                                                                                                               ctGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCTGTATGTGCACCCAAg   >  2:1511738/1‑150 (MQ=255)
                                                                                                                                                tGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCTGTATGTGCACCCAAgg  >  2:457234/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCTGTATGTGCACCCAAGG  >  CP009273/2454150‑2454443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCTGTATGTGCACCCAA  >  CP009273/2454152‑2454441
                                                                                                                                                 |                                                                                                                                                
TCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTG                                                                                                                                              <  SRR19973074.4165144/150‑1 (MQ=60)
TCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTG                                                                                                                                              <  SRR19973074.3031124/150‑1 (MQ=60)
     ATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGG                                                                                                                                         >  SRR19973074.771508/1‑150 (MQ=60)
     ATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGG                                                                                                                                         >  SRR19973074.771492/1‑150 (MQ=60)
          AGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGC                                                                                                                                    >  SRR19973074.358218/1‑150 (MQ=60)
          AGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGC                                                                                                                                    >  SRR19973074.358251/1‑150 (MQ=60)
           GCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCA                                                                                                                                   <  SRR19973074.2970677/150‑1 (MQ=60)
              TCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAAC                                                                                                                                <  SRR19973074.1214056/150‑1 (MQ=60)
                GCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCTTCTGCGCGGTTTGCAAACAC                                                                                                                              <  SRR19973074.1858539/150‑1 (MQ=60)
                  AGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGC                                                                                                                            >  SRR19973074.3073628/1‑150 (MQ=60)
                  AGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGC                                                                                                                            <  SRR19973074.2823159/150‑1 (MQ=60)
                  AGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGC                                                                                                                            <  SRR19973074.2823150/150‑1 (MQ=60)
                    GCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGG                                                                                                                          <  SRR19973074.645302/150‑1 (MQ=60)
                     CAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGC                                                                                                                         <  SRR19973074.1214655/150‑1 (MQ=60)
                     CAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGC                                                                                                                         <  SRR19973074.1214737/150‑1 (MQ=60)
                     CAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGC                                                                                                                         <  SRR19973074.1214750/150‑1 (MQ=60)
                     CAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGC                                                                                                                         >  SRR19973074.1476365/1‑150 (MQ=60)
                      AAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCT                                                                                                                        >  SRR19973074.996707/1‑150 (MQ=60)
                      AAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCT                                                                                                                        >  SRR19973074.3733436/1‑150 (MQ=60)
                        AAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGT                                                                                                                      >  SRR19973074.1878353/1‑150 (MQ=60)
                             AAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGAC                                                                                                                 <  SRR19973074.1963567/150‑1 (MQ=60)
                             AAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGAC                                                                                                                 <  SRR19973074.4066111/150‑1 (MQ=60)
                              AATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACG                                                                                                                >  SRR19973074.888140/1‑150 (MQ=60)
                               ATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGC                                                                                                               >  SRR19973074.1498792/1‑150 (MQ=60)
                                TCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCG                                                                                                              <  SRR19973074.48419/150‑1 (MQ=60)
                                  GATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGT                                                                                                            <  SRR19973074.1994154/150‑1 (MQ=60)
                                  GATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGT                                                                                                            <  SRR19973074.1773125/150‑1 (MQ=60)
                                  GATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGT                                                                                                            <  SRR19973074.2219734/150‑1 (MQ=60)
                                    TATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGC                                                                                                          <  SRR19973074.3527276/150‑1 (MQ=60)
                                    TATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGC                                                                                                          <  SRR19973074.187300/150‑1 (MQ=60)
                                    TATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGC                                                                                                          <  SRR19973074.718568/150‑1 (MQ=60)
                                        CAGGCTCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATGCCACGCTACTGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTC                                                                                                      <  SRR19973074.2233987/150‑1 (MQ=60)
                                        CAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTC                                                                                                      <  SRR19973074.2234004/150‑1 (MQ=60)
                                         AGGCGCACACCGTCAGACTCTTCAGTGAAANTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGGTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCG                                                                                                     >  SRR19973074.3861641/1‑150 (MQ=60)
                                         AGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCG                                                                                                     <  SRR19973074.3334895/150‑1 (MQ=60)
                                         AGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCG                                                                                                     <  SRR19973074.3335049/150‑1 (MQ=60)
                                         AGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCG                                                                                                     >  SRR19973074.2409114/1‑150 (MQ=60)
                                             GCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGT                                                                                                 >  SRR19973074.3059798/1‑150 (MQ=60)
                                             GCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGT                                                                                                 >  SRR19973074.2184268/1‑150 (MQ=60)
                                             GCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGAGTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGT                                                                                                 <  SRR19973074.996963/150‑1 (MQ=60)
                                              CACACCTTCAGTCTCTTCATTGATAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTT                                                                                                <  SRR19973074.1730/150‑1 (MQ=60)
                                              CACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTT                                                                                                <  SRR19973074.698013/150‑1 (MQ=60)
                                               ACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTG                                                                                               >  SRR19973074.1412405/1‑150 (MQ=60)
                                                CACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGT                                                                                              >  SRR19973074.2205476/1‑150 (MQ=60)
                                                CACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGT                                                                                              <  SRR19973074.3575209/150‑1 (MQ=60)
                                                    GTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCAT                                                                                          <  SRR19973074.136725/150‑1 (MQ=60)
                                                      CAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAA                                                                                        >  SRR19973074.3045366/1‑150 (MQ=60)
                                                      CAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAA                                                                                        >  SRR19973074.1829422/1‑150 (MQ=60)
                                                       AGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAAT                                                                                       <  SRR19973074.3882299/150‑1 (MQ=60)
                                                       AGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAAT                                                                                       >  SRR19973074.513071/1‑150 (MQ=60)
                                                       AGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAAT                                                                                       >  SRR19973074.1204051/1‑150 (MQ=60)
                                                        GACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATG                                                                                      >  SRR19973074.1261660/1‑150 (MQ=60)
                                                        GACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATG                                                                                      >  SRR19973074.1261685/1‑150 (MQ=60)
                                                         ACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGG                                                                                     <  SRR19973074.3788655/150‑1 (MQ=60)
                                                          CTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGT                                                                                    >  SRR19973074.58815/1‑150 (MQ=60)
                                                             TTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCC                                                                                 >  SRR19973074.71663/1‑150 (MQ=60)
                                                               CAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAA                                                                               <  SRR19973074.2534533/150‑1 (MQ=60)
                                                               CAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAA                                                                               <  SRR19973074.2534334/150‑1 (MQ=60)
                                                                AGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAAC                                                                              <  SRR19973074.1583412/150‑1 (MQ=60)
                                                                   GAAACTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATC                                                                           >  SRR19973074.258134/1‑150 (MQ=60)
                                                                     AAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCA                                                                         >  SRR19973074.2118857/1‑150 (MQ=60)
                                                                      AGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCAT                                                                        <  SRR19973074.786450/150‑1 (MQ=60)
                                                                      AGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCAT                                                                        <  SRR19973074.786393/150‑1 (MQ=60)
                                                                       GTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATA                                                                       <  SRR19973074.708889/150‑1 (MQ=60)
                                                                         CGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACA                                                                     <  SRR19973074.345078/150‑1 (MQ=60)
                                                                         CGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACA                                                                     >  SRR19973074.2405467/1‑150 (MQ=60)
                                                                          GGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAG                                                                    >  SRR19973074.1639458/1‑150 (MQ=60)
                                                                          GGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAG                                                                    >  SRR19973074.1639489/1‑150 (MQ=60)
                                                                            GGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCA                                                                  >  SRR19973074.1994978/1‑150 (MQ=60)
                                                                            GGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCA                                                                  <  SRR19973074.3380737/150‑1 (MQ=60)
                                                                             GTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAG                                                                 >  SRR19973074.3784671/1‑150 (MQ=60)
                                                                             GTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAG                                                                 <  SRR19973074.2629878/150‑1 (MQ=60)
                                                                              TGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGC                                                                <  SRR19973074.1621999/150‑1 (MQ=60)
                                                                              TGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGC                                                                <  SRR19973074.1622042/150‑1 (MQ=60)
                                                                               GATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCA                                                               <  SRR19973074.3773283/150‑1 (MQ=60)
                                                                               GATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCA                                                               <  SRR19973074.2926150/150‑1 (MQ=60)
                                                                               GATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCA                                                               <  SRR19973074.2454175/150‑1 (MQ=60)
                                                                                ATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAA                                                              <  SRR19973074.1413876/150‑1 (MQ=60)
                                                                                    TGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCG                                                          <  SRR19973074.1520466/150‑1 (MQ=60)
                                                                                    TGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCG                                                          <  SRR19973074.1898393/150‑1 (MQ=60)
                                                                                     GCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGG                                                         <  SRR19973074.398958/150‑1 (MQ=60)
                                                                                     GCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGG                                                         <  SRR19973074.4106073/150‑1 (MQ=60)
                                                                                     GCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGG                                                         <  SRR19973074.399054/150‑1 (MQ=60)
                                                                                         GGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTT                                                     <  SRR19973074.3552229/150‑1 (MQ=60)
                                                                                          GTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTT                                                    <  SRR19973074.2620862/150‑1 (MQ=60)
                                                                                              AGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATC                                                >  SRR19973074.472652/1‑150 (MQ=60)
                                                                                              AGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATC                                                >  SRR19973074.472643/1‑150 (MQ=60)
                                                                                              AGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATC                                                >  SRR19973074.472584/1‑150 (MQ=60)
                                                                                               GATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCA                                               <  SRR19973074.2015149/150‑1 (MQ=60)
                                                                                               GATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCA                                               <  SRR19973074.2015193/150‑1 (MQ=60)
                                                                                               GATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCA                                               <  SRR19973074.2015045/150‑1 (MQ=60)
                                                                                               GATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCA                                               <  SRR19973074.3496383/150‑1 (MQ=60)
                                                                                                 TTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGC                                             >  SRR19973074.1700025/1‑150 (MQ=60)
                                                                                                    CACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACT                                          >  SRR19973074.2083783/1‑150 (MQ=60)
                                                                                                     ACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGACAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTA                                         >  SRR19973074.894303/1‑150 (MQ=60)
                                                                                                       GCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACA                                       >  SRR19973074.1923379/1‑150 (MQ=60)
                                                                                                       GCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACA                                       >  SRR19973074.190729/1‑150 (MQ=60)
                                                                                                          ACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTT                                    <  SRR19973074.1610206/150‑1 (MQ=60)
                                                                                                            GGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTAC                                  >  SRR19973074.394265/1‑150 (MQ=60)
                                                                                                            GGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTAC                                  >  SRR19973074.2528956/1‑150 (MQ=60)
                                                                                                                TTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCAT                              <  SRR19973074.3416653/150‑1 (MQ=60)
                                                                                                                  TTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCG                            >  SRR19973074.3698388/1‑150 (MQ=60)
                                                                                                                     CAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTG                         >  SRR19973074.1062182/1‑150 (MQ=60)
                                                                                                                     CAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTG                         >  SRR19973074.273131/1‑150 (MQ=60)
                                                                                                                     CAGTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTG                         >  SRR19973074.1898302/1‑150 (MQ=60)
                                                                                                                      AGTCTGCGCCGCCTGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGTAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTTCTCATCGTTGA                        <  SRR19973074.599719/150‑1 (MQ=60)
                                                                                                                       GTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGAT                       <  SRR19973074.1243908/150‑1 (MQ=60)
                                                                                                                       GTCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTGTCATCAGCACTACATTTACTCATCGTTGAT                       <  SRR19973074.2143459/150‑1 (MQ=60)
                                                                                                                        TCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATT                      <  SRR19973074.259001/150‑1 (MQ=60)
                                                                                                                        TCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATT                      <  SRR19973074.1924308/150‑1 (MQ=60)
                                                                                                                        TCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATT                      <  SRR19973074.3207308/150‑1 (MQ=60)
                                                                                                                        TCAGCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATT                      <  SRR19973074.3207285/150‑1 (MQ=60)
                                                                                                                           GCGCCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCC                   <  SRR19973074.991221/150‑1 (MQ=60)
                                                                                                                              CCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCT                <  SRR19973074.2904713/150‑1 (MQ=60)
                                                                                                                              CCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCT                >  SRR19973074.2672368/1‑150 (MQ=60)
                                                                                                                              CCGCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCT                >  SRR19973074.422563/1‑150 (MQ=60)
                                                                                                                                GCCAGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCTGT              >  SRR19973074.3433478/1‑150 (MQ=60)
                                                                                                                                   AGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCTTTATG           >  SRR19973074.3877162/1‑150 (MQ=60)
                                                                                                                                   AGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCTTTATG           >  SRR19973074.3877237/1‑150 (MQ=60)
                                                                                                                                   AGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCTGTATG           >  SRR19973074.2684682/1‑150 (MQ=60)
                                                                                                                                   AGCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCTGTATG           >  SRR19973074.2684669/1‑150 (MQ=60)
                                                                                                                                    GCGTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCTGTATGT          <  SRR19973074.635729/150‑1 (MQ=60)
                                                                                                                                      GTTTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCTGTATGTGC        >  SRR19973074.99412/1‑150 (MQ=60)
                                                                                                                                        TTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCTGTATGTGCAC      >  SRR19973074.3983125/1‑150 (MQ=60)
                                                                                                                                        TTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCTGTATGTGCAC      >  SRR19973074.3983126/1‑150 (MQ=60)
                                                                                                                                        TTGCTCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCTGTATGTGCAC      >  SRR19973074.3983202/1‑150 (MQ=60)
                                                                                                                                            TCTGCGTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCTGTATGTGCACCCAA  >  SRR19973074.2613695/1‑150 (MQ=60)
                                                                                                                                                 |                                                                                                                                                
TCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGTTCAGATTCCACGCTACGGGCTTTTTCAGTCAGCGCCGCCAGCGTTTGCTCTGCTTCTGCGCGGTTTGCAAACACGCGGCTGTAAGACGCGGTGCAGTCGGAGTTGTCCATAATGGTGCCAACATCCATACAGCAGCAAACCGGGGTTTCATCAGCACTACATTTACTCATCGTTGATTTCCTCTGTATGTGCACCCAA  >  CP009273/2454152‑2454441

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑