Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,645,592 C→A 94.9% D19Y (GAC→TAC)  yfhM ← bacterial alpha2‑macroglobulin colonization factor ECAM; anti‑host protease defense factor; periplasmic inner membrane‑anchored lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,645,5920CA94.9% 625.5 / 23.5 214D19Y (GAC→TAC) yfhMbacterial alpha2‑macroglobulin colonization factor ECAM; anti‑host protease defense factor; periplasmic inner membrane‑anchored lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (5/6);  new base A (93/110);  total (98/116)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01

Only 100 of 214 total aligned reads displayed.

AGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCATA  >  CP009273/2645447‑2645736
                                                                                                                                                 |                                                                                                                                                
 gCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCa                                                                                                                                               >  2:1263126/1‑148 (MQ=255)
      ttACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTg                                                                                                                                          >  1:606954/1‑148 (MQ=255)
        aCCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTgcc                                                                                                                                        >  2:3429564/1‑148 (MQ=255)
               ctctGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTgccagcgcca                                                                                                                                 >  2:2976614/1‑148 (MQ=255)
                tctGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTgccagcgccag                                                                                                                                >  1:1523850/1‑148 (MQ=255)
                 ctGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTgccagcgccagc                                                                                                                               <  1:2459310/148‑1 (MQ=255)
                 ctGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTgccagcgccagc                                                                                                                               <  1:3092240/148‑1 (MQ=255)
                  tGTTGGGCCAGTTTTCGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCa                                                                                                                              <  1:2961989/148‑1 (MQ=255)
                    ttGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCAtt                                                                                                                            >  1:1962263/1‑148 (MQ=255)
                         ccAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCAt                                                                                                                       >  1:319399/1‑148 (MQ=255)
                         ccAGTTTGTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCAt                                                                                                                       <  1:3024271/148‑1 (MQ=255)
                             tttttGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAg                                                                                                                   <  1:312162/148‑1 (MQ=255)
                             tttttGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAg                                                                                                                   <  2:1272102/148‑1 (MQ=255)
                             tttttGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAg                                                                                                                   <  2:1962242/148‑1 (MQ=255)
                              ttttGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAgg                                                                                                                  <  2:2666292/148‑1 (MQ=255)
                                 tGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAggcgg                                                                                                               <  2:2929867/148‑1 (MQ=255)
                                    ctctcCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTa                                                                                                            <  2:482079/148‑1 (MQ=255)
                                         cGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGt                                                                                                       <  2:2550302/148‑1 (MQ=255)
                                         cGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAAATTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGt                                                                                                       <  1:690892/148‑1 (MQ=255)
                                           gCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTaa                                                                                                     <  2:771629/148‑1 (MQ=255)
                                           gCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTaa                                                                                                     <  1:486860/148‑1 (MQ=255)
                                               ggAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACttt                                                                                                 >  1:2474753/1‑148 (MQ=255)
                                                  gagTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACtttttc                                                                                              >  2:1723107/1‑148 (MQ=255)
                                                   agTTTTTCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCATCATTAGCATGCAGGCGGCTACGCGTAACtttttca                                                                                             <  1:44166/148‑1 (MQ=255)
                                                   agTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACtttttca                                                                                             >  2:37902/1‑148 (MQ=255)
                                                   agTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACtttttca                                                                                             <  1:1864038/148‑1 (MQ=255)
                                                   agTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACtttttca                                                                                             >  2:1838544/1‑148 (MQ=255)
                                                   agTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACtttttca                                                                                             <  1:2260059/148‑1 (MQ=255)
                                                    gTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACtttttcat                                                                                            <  1:1373499/148‑1 (MQ=255)
                                                    gTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACtttttcat                                                                                            <  1:1779057/148‑1 (MQ=255)
                                                    gTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACtttttcat                                                                                            <  2:155939/148‑1 (MQ=255)
                                                     ttttGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACtttttcatt                                                                                           >  2:1682322/1‑148 (MQ=255)
                                                     ttttGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACtttttcatt                                                                                           >  2:1681383/1‑148 (MQ=255)
                                                      tttGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACtttttcattt                                                                                          <  1:1256537/148‑1 (MQ=255)
                                                         gCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACtttttcatttttc                                                                                       <  1:1906612/148‑1 (MQ=255)
                                                         gCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACtttttcatttttc                                                                                       <  1:1843457/148‑1 (MQ=255)
                                                           tGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTttttcatttttcat                                                                                     <  1:2330871/148‑1 (MQ=255)
                                                             aaCTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATcc                                                                                   >  1:3502803/1‑148 (MQ=255)
                                                             aaCTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATcc                                                                                   <  1:372318/148‑1 (MQ=255)
                                                             aaCTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTGTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATcc                                                                                   <  2:3350974/148‑1 (MQ=255)
                                                              aCTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATccc                                                                                  <  1:1841201/148‑1 (MQ=255)
                                                                   cgTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCca                                                                             >  1:2421852/1‑148 (MQ=255)
                                                                     ttttCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCcaca                                                                           <  2:2943551/148‑1 (MQ=255)
                                                                     ttttCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCcaca                                                                           >  2:820909/1‑148 (MQ=255)
                                                                     ttttCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCACTGCcaca                                                                           >  1:1442682/1‑148 (MQ=255)
                                                                        tCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATg                                                                        <  1:424913/148‑1 (MQ=255)
                                                                          tGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGc                                                                      <  1:812633/148‑1 (MQ=255)
                                                                           gAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGcc                                                                     <  2:2878048/148‑1 (MQ=255)
                                                                            aaGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGccc                                                                    >  1:2689743/1‑148 (MQ=255)
                                                                             agagGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCg                                                                   <  1:1408002/148‑1 (MQ=255)
                                                                             agagGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCg                                                                   >  1:3208920/1‑148 (MQ=255)
                                                                              gagGCCGCTTTAGTAACTTCAGAAGGCGCATC‑TTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGtt                                                                 >  2:1055795/1‑148 (MQ=255)
                                                                                ggCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACTATGGCCCGTTa                                                                <  1:1575918/148‑1 (MQ=255)
                                                                                  ccGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGc                                                              <  2:866774/148‑1 (MQ=255)
                                                                                   cGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCa                                                             <  1:1640211/148‑1 (MQ=255)
                                                                                   cGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCa                                                             <  1:2711638/148‑1 (MQ=255)
                                                                                       ttAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGt                                                         >  2:1318875/1‑148 (MQ=255)
                                                                                       ttAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGt                                                         >  2:1977029/1‑148 (MQ=255)
                                                                                          gTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGa                                                      <  2:2288269/148‑1 (MQ=255)
                                                                                          gTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGa                                                      >  1:1252529/1‑148 (MQ=255)
                                                                                           tAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGaa                                                     >  1:2924727/1‑148 (MQ=255)
                                                                                            aaCTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGaat                                                    <  2:826968/148‑1 (MQ=255)
                                                                                            aaCTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGaat                                                    >  2:2701211/1‑148 (MQ=255)
                                                                                             aCTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGaata                                                   >  2:3379043/1‑148 (MQ=255)
                                                                                             aCTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCAAAATGGCCCGTTAGCAACGTCGaata                                                   <  1:2140081/148‑1 (MQ=255)
                                                                                                 cAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAatta                                               <  2:1300029/148‑1 (MQ=255)
                                                                                                 cAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAatta                                               <  2:3070348/148‑1 (MQ=255)
                                                                                                 cAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAatta                                               <  1:6555/148‑1 (MQ=255)
                                                                                                   gAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAattatt                                             <  1:2055607/148‑1 (MQ=255)
                                                                                                   gAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAattatt                                             <  2:898456/148‑1 (MQ=255)
                                                                                                    aaGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAAttatta                                            >  2:3352322/1‑148 (MQ=255)
                                                                                                    aaGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAAttatta                                            >  2:1000855/1‑148 (MQ=255)
                                                                                                    aaGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGGAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAAttatta                                            <  2:713868/148‑1 (MQ=255)
                                                                                                      ggCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATg                                          <  1:1055572/148‑1 (MQ=255)
                                                                                                      ggCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATg                                          >  2:148196/1‑148 (MQ=255)
                                                                                                       gcgcATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGc                                         <  2:2503034/148‑1 (MQ=255)
                                                                                                       gcgcATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGc                                         >  1:1735951/1‑148 (MQ=255)
                                                                                                          cATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTg                                      >  1:1321050/1‑148 (MQ=255)
                                                                                                          cATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCACGTTAGCAACGTCGAATAATTATTATGCGTg                                      >  2:1380463/1‑148 (MQ=255)
                                                                                                            tCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTgag                                    >  2:115058/1‑148 (MQ=255)
                                                                                                             cTTTTTTCACCTCTGTTGGCGCTTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGAAGGCGGCTACGAGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCTTTAGCAACGTAGAATAATTATTATGCGTgaga                                   <  2:2529194/148‑1 (MQ=255)
                                                                                                               tttttCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAAt                                 >  1:1682446/1‑148 (MQ=255)
                                                                                                                   tCACCGCTGTTGGTGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCAAAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTAc                             <  2:2105863/148‑1 (MQ=255)
                                                                                                                    cACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACg                            <  2:2754073/148‑1 (MQ=255)
                                                                                                                     aCCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGt                           <  2:2456737/148‑1 (MQ=255)
                                                                                                                     aCCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGt                           <  2:672653/148‑1 (MQ=255)
                                                                                                                     aCCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGt                           <  1:1682308/148‑1 (MQ=255)
                                                                                                                      ccGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGtt                          >  1:3305051/1‑148 (MQ=255)
                                                                                                                      ccGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGtt                          >  1:3287034/1‑148 (MQ=255)
                                                                                                                      ccGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGtt                          <  1:2608837/148‑1 (MQ=255)
                                                                                                                      ccGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGtt                          >  2:399700/1‑148 (MQ=255)
                                                                                                                          tGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGt                      <  1:1639111/148‑1 (MQ=255)
                                                                                                                               gcgcGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTc                 >  1:1020819/1‑148 (MQ=255)
                                                                                                                                  cgTTATCGTTGTTGTCGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGt              >  2:2072494/1‑148 (MQ=255)
                                                                                                                                   gTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTa             >  2:3179833/1‑148 (MQ=255)
                                                                                                                                        cGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACg        >  2:2194490/1‑148 (MQ=255)
                                                                                                                                          ttgttgTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGcc      >  2:1702133/1‑148 (MQ=255)
                                                                                                                                          ttgttgTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGcc      >  2:2261864/1‑148 (MQ=255)
                                                                                                                                             ttgtAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCAt   <  1:2164604/148‑1 (MQ=255)
                                                                                                                                              tgtAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCATa  <  2:2005136/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
AGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCATA  >  CP009273/2645447‑2645736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCATAC  >  CP009273/2645449‑2645737
                                                                                                                                               |                                                                                                                                                 
CACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCC                                                                                                                                             >  SRR19973067.2275662/1‑150 (MQ=60)
  cgtttCCGGCACTCTGTTTGGCGATTTTTTGTCTCTTCGGCTCGGAGAGTTTTTCTGTACTCGCGTTTTCTGAAGAGGCGGATTTTGTAAATTCAGAAGTCGCATCTTTTTTCAACGCTGTTGGCGCGTTATCGTTGTTGTAGCACCATG                                                                                                                                           <  SRR19973067.1770356/145‑1 (MQ=60)
  CCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTG                                                                                                                                           >  SRR19973067.610845/1‑150 (MQ=60)
       CCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTGCCAGC                                                                                                                                      >  SRR19973067.2472892/1‑150 (MQ=60)
       CCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTGCCAGC                                                                                                                                      >  SRR19973067.677261/1‑150 (MQ=60)
            ACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAG                                                                                                                                 >  SRR19973067.1534086/1‑150 (MQ=60)
               CTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCAT                                                                                                                              <  SRR19973067.3111909/150‑1 (MQ=60)
               CTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCAT                                                                                                                              <  SRR19973067.2475478/150‑1 (MQ=60)
                TGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATT                                                                                                                             >  SRR19973067.1975595/1‑150 (MQ=60)
                TGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATT                                                                                                                             >  SRR19973067.1975574/1‑150 (MQ=60)
                TGTTGGGCCAGTTTTCGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATT                                                                                                                             <  SRR19973067.2980700/150‑1 (MQ=60)
                     GGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCAT                                                                                                                        >  SRR19973067.321557/1‑150 (MQ=60)
                       CCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGC                                                                                                                      <  SRR19973067.1262224/150‑1 (MQ=60)
                       CCAGTTTGTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGC                                                                                                                      <  SRR19973067.3043524/150‑1 (MQ=60)
                        CAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCA                                                                                                                     <  SRR19973067.272815/150‑1 (MQ=60)
                           TTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGC                                                                                                                  <  SRR19973067.314225/150‑1 (MQ=60)
                           TTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGC                                                                                                                  <  SRR19973067.314220/150‑1 (MQ=60)
                                 TCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTAC                                                                                                            >  SRR19973067.174432/1‑150 (MQ=60)
                                     TCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGT                                                                                                        >  SRR19973067.658051/1‑150 (MQ=60)
                                     TCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGT                                                                                                        >  SRR19973067.3129622/1‑150 (MQ=60)
                                       CGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAAATTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAA                                                                                                      <  SRR19973067.695653/150‑1 (MQ=60)
                                        GGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAAC                                                                                                     <  SRR19973067.1475098/150‑1 (MQ=60)
                                        GGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAAC                                                                                                     >  SRR19973067.579946/1‑150 (MQ=60)
                                         GCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACT                                                                                                    <  SRR19973067.490211/150‑1 (MQ=60)
                                         GCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACT                                                                                                    <  SRR19973067.489581/150‑1 (MQ=60)
                                         GCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACT                                                                                                    <  SRR19973067.3324238/150‑1 (MQ=60)
                                           ACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTT                                                                                                  >  SRR19973067.2491016/1‑150 (MQ=60)
                                           ACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTT                                                                                                  >  SRR19973067.2491025/1‑150 (MQ=60)
                                             GGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTT                                                                                                >  SRR19973067.1850759/1‑150 (MQ=60)
                                                 AGTTTTTCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCATCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATT                                                                                            <  SRR19973067.44774/150‑1 (MQ=60)
                                                 AGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATT                                                                                            <  SRR19973067.1876757/150‑1 (MQ=60)
                                                 AGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATT                                                                                            <  SRR19973067.2275051/150‑1 (MQ=60)
                                                  GTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTT                                                                                           <  SRR19973067.1382875/150‑1 (MQ=60)
                                                  GTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTT                                                                                           <  SRR19973067.1791096/150‑1 (MQ=60)
                                                    TTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTT                                                                                         <  SRR19973067.1265042/150‑1 (MQ=60)
                                                       GCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAATGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTGGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCAT                                                                                      <  SRR19973067.3303503/150‑1 (MQ=60)
                                                       GCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCAT                                                                                      <  SRR19973067.1856029/150‑1 (MQ=60)
                                                       GCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCAT                                                                                      <  SRR19973067.2038697/150‑1 (MQ=60)
                                                       GCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCAT                                                                                      <  SRR19973067.1919611/150‑1 (MQ=60)
                                                       GCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCAT                                                                                      <  SRR19973067.1918998/150‑1 (MQ=60)
                                                       GCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCAT                                                                                      <  SRR19973067.1855862/150‑1 (MQ=60)
                                                         TGATCTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCC                                                                                    <  SRR19973067.400824/150‑1 (MQ=60)
                                                         TGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCC                                                                                    <  SRR19973067.2346166/150‑1 (MQ=60)
                                                         TGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCC                                                                                    <  SRR19973067.2346163/150‑1 (MQ=60)
                                                         TGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCC                                                                                    >  SRR19973067.3525004/1‑150 (MQ=60)
                                                           AACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCT                                                                                  <  SRR19973067.374911/150‑1 (MQ=60)
                                                            ACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTG                                                                                 <  SRR19973067.1857402/150‑1 (MQ=60)
                                                            ACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTG                                                                                 <  SRR19973067.1853761/150‑1 (MQ=60)
                                                            ACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAAATACACAAGGAGCATATTTTTTCACCGCTGTTTGTGCGTTATCGTTGTTTTATCACCCTGCCAGCGCCAGCAATAGCATTAAGGCGGCTACGCGTAAATTTTTCATTTTTCATCCCTG                                                                                 >  SRR19973067.2119843/1‑150 (MQ=60)
                                                             CTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGC                                                                                >  SRR19973067.2547208/1‑150 (MQ=60)
                                                               CGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCA                                                                              >  SRR19973067.2955009/1‑150 (MQ=60)
                                                               CGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCA                                                                              >  SRR19973067.2437733/1‑150 (MQ=60)
                                                                 CGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCACTGCCACA                                                                            >  SRR19973067.1452380/1‑150 (MQ=60)
                                                                      TCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGC                                                                       <  SRR19973067.427866/150‑1 (MQ=60)
                                                                      TCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGC                                                                       <  SRR19973067.428115/150‑1 (MQ=60)
                                                                       CTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCC                                                                      <  SRR19973067.2212711/150‑1 (MQ=60)
                                                                        TGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCC                                                                     <  SRR19973067.818209/150‑1 (MQ=60)
                                                                        TGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTCCGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCC                                                                     >  SRR19973067.2706426/1‑150 (MQ=60)
                                                                        TGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCC                                                                     >  SRR19973067.2707021/1‑150 (MQ=60)
                                                                         GAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCG                                                                    >  SRR19973067.3225265/1‑150 (MQ=60)
                                                                         GAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCG                                                                    >  SRR19973067.3229142/1‑150 (MQ=60)
                                                                           AGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTT                                                                  <  SRR19973067.1417540/150‑1 (MQ=60)
                                                                              GGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACTATGGCCCGTTAGC                                                               <  SRR19973067.1586708/150‑1 (MQ=60)
                                                                                 CGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAAC                                                            <  SRR19973067.1651507/150‑1 (MQ=60)
                                                                                 CGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAAC                                                            <  SRR19973067.2729113/150‑1 (MQ=60)
                                                                                  GCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACG                                                           >  SRR19973067.415413/1‑150 (MQ=60)
                                                                                  GCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCATGCCACAAAGGCCCGTTAGCAACG                                                           >  SRR19973067.989115/1‑150 (MQ=60)
                                                                                      TAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGA                                                       >  SRR19973067.1260992/1‑150 (MQ=60)
                                                                                       AGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAA                                                      >  SRR19973067.2943314/1‑150 (MQ=60)
                                                                                           ACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAAT                                                  <  SRR19973067.1040246/150‑1 (MQ=60)
                                                                                           ACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCAAAATGGCCCGTTAGCAACGTCGAATAAT                                                  <  SRR19973067.2154386/150‑1 (MQ=60)
                                                                                            CTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATT                                                 >  SRR19973067.2502699/1‑150 (MQ=60)
                                                                                            CTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATT                                                 >  SRR19973067.707532/1‑150 (MQ=60)
                                                                                            CTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATT                                                 <  SRR19973067.1145641/150‑1 (MQ=60)
                                                                                               CAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATT                                              <  SRR19973067.1568200/150‑1 (MQ=60)
                                                                                               CAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATT                                              <  SRR19973067.3451389/150‑1 (MQ=60)
                                                                                               CAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATT                                              <  SRR19973067.6652/150‑1 (MQ=60)
                                                                                                 GAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTAT                                            <  SRR19973067.2069313/150‑1 (MQ=60)
                                                                                                   AGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGC                                          >  SRR19973067.1747993/1‑150 (MQ=60)
                                                                                                   AGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGC                                          >  SRR19973067.1747826/1‑150 (MQ=60)
                                                                                                   AGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGC                                          >  SRR19973067.2758539/1‑150 (MQ=60)
                                                                                                    GGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGNG                                         <  SRR19973067.1062809/150‑1 (MQ=60)
                                                                                                      CGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTG                                       >  SRR19973067.1330042/1‑150 (MQ=60)
                                                                                                      CGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTG                                       >  SRR19973067.1330070/1‑150 (MQ=60)
                                                                                                           CTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAAT                                  >  SRR19973067.1694048/1‑150 (MQ=60)
                                                                                                            TTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATT                                 <  SRR19973067.3056140/150‑1 (MQ=60)
                                                                                                              TTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTA                               <  SRR19973067.1340099/150‑1 (MQ=60)
                                                                                                               TTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTAC                              <  SRR19973067.1460059/150‑1 (MQ=60)
                                                                                                                  CACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTT                           >  SRR19973067.3307678/1‑150 (MQ=60)
                                                                                                                  CACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTT                           >  SRR19973067.3325901/1‑150 (MQ=60)
                                                                                                                  CACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTT                           <  SRR19973067.139432/150‑1 (MQ=60)
                                                                                                                   ACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTA                          <  SRR19973067.1692973/150‑1 (MQ=60)
                                                                                                                   ACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTA                          <  SRR19973067.1693909/150‑1 (MQ=60)
                                                                                                                   ACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTA                          <  SRR19973067.1693923/150‑1 (MQ=60)
                                                                                                                    CCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTAT                         <  SRR19973067.2625782/150‑1 (MQ=60)
                                                                                                                        TGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAA                     <  SRR19973067.1650371/150‑1 (MQ=60)
                                                                                                                        TGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAA                     <  SRR19973067.1650390/150‑1 (MQ=60)
                                                                                                                        TGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAA                     <  SRR19973067.1650519/150‑1 (MQ=60)
                                                                                                                        TGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAA                     >  SRR19973067.3544173/1‑150 (MQ=60)
                                                                                                                           TGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTC                  >  SRR19973067.1027830/1‑150 (MQ=60)
                                                                                                                                CGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTAT             <  SRR19973067.2995394/150‑1 (MQ=60)
                                                                                                                                  TTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCA           >  SRR19973067.1442429/1‑150 (MQ=60)
                                                                                                                                  TTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCA           >  SRR19973067.3118679/1‑150 (MQ=60)
                                                                                                                                   TATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGACTTTACGTTATGTAATTCAGTATCAC          >  SRR19973067.943646/1‑150 (MQ=60)
                                                                                                                                           TTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCATAC  <  SRR19973067.2093650/150‑1 (MQ=60)
                                                                                                                                           TTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCATAC  <  SRR19973067.2179070/150‑1 (MQ=60)
                                                                                                                                           TTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCATAC  <  SRR19973067.2179074/150‑1 (MQ=60)
                                                                                                                                               |                                                                                                                                                 
CACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCATAC  >  CP009273/2645449‑2645737

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑