Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F534 I1 R1	1235	230.2	7611948	95.1%	7238962	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	2,645,592	C→A	D19Y (GAC→TAC)	yfhM ←	bacterial alpha2‑macroglobulin colonization factor ECAM; anti‑host protease defense factor; periplasmic inner membrane‑anchored lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,645,592	0	C	A	99.5%	690.7 / NA	209	D19Y (GAC→TAC)	yfhM	bacterial alpha2‑macroglobulin colonization factor ECAM; anti‑host protease defense factor; periplasmic inner membrane‑anchored lipoprotein
Reads supporting (aligned to +/- strand): ref base C (0/1); new base A (89/119); total (89/120)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 209 total aligned reads displayed.

TCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCA  >  CP009273/2645445‑2645734

 cAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCa                                                                                                                                             >  2:3324723/1‑150 (MQ=255)

   gCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCAcc                                                                                                                                           >  1:2872999/1‑150 (MQ=255)

     aCCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCt                                                                                                                                         >  2:2276944/1‑150 (MQ=255)

     aCCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCt                                                                                                                                         >  1:3680031/1‑150 (MQ=255)

       cTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTgc                                                                                                                                       <  1:90448/150‑1 (MQ=255)

       cTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTgc                                                                                                                                       >  1:1960686/1‑150 (MQ=255)

         tACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTgcca                                                                                                                                     <  1:2247091/150‑1 (MQ=255)

            cGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTgccagcg                                                                                                                                  >  1:2627167/1‑150 (MQ=255)

               cACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTgccagcgcca                                                                                                                               <  2:758675/150‑1 (MQ=255)

               cACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTgccagcgcca                                                                                                                               <  1:186787/150‑1 (MQ=255)

                aCTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTgccagcgccag                                                                                                                              <  2:2624288/150‑1 (MQ=255)

                   ctGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCAt                                                                                                                           <  1:2904500/150‑1 (MQ=255)

                      ttGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAg                                                                                                                        <  1:3162638/150‑1 (MQ=255)

                      ttGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAg                                                                                                                        <  1:3196804/150‑1 (MQ=255)

                       tGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGc                                                                                                                       >  2:3597124/1‑150 (MQ=255)

                        gggCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCa                                                                                                                      >  1:3154161/1‑150 (MQ=255)

                           ccAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGc                                                                                                                   >  1:2106611/1‑150 (MQ=255)

                           ccAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGc                                                                                                                   >  2:560116/1‑150 (MQ=255)

                             aGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAg                                                                                                                 >  1:2276864/1‑150 (MQ=255)

                             aGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCA‑TAGCATGCAgg                                                                                                                >  1:3181093/1‑150 (MQ=255)

                                ttttGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAggcg                                                                                                              >  1:1611978/1‑150 (MQ=255)

                                   tGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCt                                                                                                           >  1:1570596/1‑150 (MQ=255)

                                     tctctcCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTAc                                                                                                         >  1:3018584/1‑150 (MQ=255)

                                      ctctcCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTAcg                                                                                                        <  2:151228/150‑1 (MQ=255)

                                      ctctcCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTAcg                                                                                                        >  2:3224172/1‑150 (MQ=255)

                                       tctcCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTCcgc                                                                                                       >  1:2763690/1‑150 (MQ=255)

                                       tctcCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTAcgc                                                                                                       >  1:3544842/1‑150 (MQ=255)

                                         tcCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGt                                                                                                     <  1:1439869/150‑1 (MQ=255)

                                          ccGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTa                                                                                                    <  1:3361383/150‑1 (MQ=255)

                                           cGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTaa                                                                                                   <  1:3091/150‑1 (MQ=255)

                                           cGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTaa                                                                                                   >  1:2213476/1‑150 (MQ=255)

                                                cGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACtttt                                                                                              <  2:3278394/150‑1 (MQ=255)

                                                 ggAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACttttt                                                                                             >  1:354098/1‑150 (MQ=255)

                                                    gagTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACtttttcat                                                                                          <  1:1901346/150‑1 (MQ=255)

                                                    gagTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACtttttcat                                                                                          <  2:3608634/150‑1 (MQ=255)

                                                     agTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACtttttcatt                                                                                         >  2:57944/1‑150 (MQ=255)

                                                       ttttGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACtttttcatttt                                                                                       <  2:2360298/150‑1 (MQ=255)

                                                        tttGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACtttttcattttt                                                                                      <  1:3273099/150‑1 (MQ=255)

                                                        tttGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACtttttcattttt                                                                                      <  1:665092/150‑1 (MQ=255)

                                                            cTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATc                                                                                  >  2:562019/1‑150 (MQ=255)

                                                            cTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATc                                                                                  <  2:634321/150‑1 (MQ=255)

                                                              gAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATccc                                                                                <  2:1151504/150‑1 (MQ=255)

                                                               aaCTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCt                                                                               <  1:3324723/150‑1 (MQ=255)

                                                                 cTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGc                                                                             >  2:370484/1‑150 (MQ=255)

                                                                     cgTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCcaca                                                                         >  2:3287416/1‑150 (MQ=255)

                                                                     cgTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCcaca                                                                         <  1:1215600/150‑1 (MQ=255)

                                                                         ttCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATgg                                                                     <  2:290760/150‑1 (MQ=255)

                                                                           cTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGcc                                                                   <  1:3049640/150‑1 (MQ=255)

                                                                              aaGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGt                                                                >  2:1192222/1‑150 (MQ=255)

                                                                               agagGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGtt                                                               <  1:1026974/150‑1 (MQ=255)

                                                                                 agGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAg                                                             <  2:3680031/150‑1 (MQ=255)

                                                                                    ccGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCaa                                                          <  2:306853/150‑1 (MQ=255)

                                                                                    ccGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCaa                                                          <  2:1502053/150‑1 (MQ=255)

                                                                                    ccGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCaa                                                          <  2:200938/150‑1 (MQ=255)

                                                                                     cGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAAc                                                         <  2:434709/150‑1 (MQ=255)

                                                                                     cGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAAc                                                         >  1:2114642/1‑150 (MQ=255)

                                                                                      gCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACg                                                        >  1:1184203/1‑150 (MQ=255)

                                                                                        tttAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTc                                                      >  2:3800231/1‑150 (MQ=255)

                                                                                        tttAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTc                                                      <  2:2114642/150‑1 (MQ=255)

                                                                                         ttAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCg                                                     <  1:2827607/150‑1 (MQ=255)

                                                                                          tAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGa                                                    >  1:829634/1‑150 (MQ=255)

                                                                                           aGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGaa                                                   >  2:1781526/1‑150 (MQ=255)

                                                                                           aGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGaa                                                   <  2:3103196/150‑1 (MQ=255)

                                                                                              aaCTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGaataa                                                <  1:1716715/150‑1 (MQ=255)

                                                                                              aaCTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGaataa                                                <  2:2085080/150‑1 (MQ=255)

                                                                                              aaCTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGaataa                                                >  1:3553680/1‑150 (MQ=255)

                                                                                               aCTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGaataat                                               <  2:2868614/150‑1 (MQ=255)

                                                                                                cTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAatt                                              <  2:70770/150‑1 (MQ=255)

                                                                                                 ttCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAatta                                             <  2:2988710/150‑1 (MQ=255)

                                                                                                   cAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAattatt                                           <  1:1795492/150‑1 (MQ=255)

                                                                                                   cAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAattatt                                           <  2:3154187/150‑1 (MQ=255)

                                                                                                   cAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAattatt                                           <  1:3506978/150‑1 (MQ=255)

                                                                                                       aGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGc                                       <  1:2161394/150‑1 (MQ=255)

                                                                                                       aGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGc                                       <  2:2703819/150‑1 (MQ=255)

                                                                                                       aGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGc                                       <  2:1901869/150‑1 (MQ=255)

                                                                                                         gcgcATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGt                                     <  2:938290/150‑1 (MQ=255)

                                                                                                             aTCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTgaga                                 >  2:1104275/1‑150 (MQ=255)

                                                                                                             aTCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGGAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGATACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTgaga                                 <  1:866512/150‑1 (MQ=255)

                                                                                                               cTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAAt                               <  2:1709357/150‑1 (MQ=255)

                                                                                                                  ttttCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTa                            >  2:2464539/1‑150 (MQ=255)

                                                                                                                  ttttCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTa                            <  2:2257524/150‑1 (MQ=255)

                                                                                                                      cACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGtt                        >  1:323506/1‑150 (MQ=255)

                                                                                                                      cACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGtt                        >  2:3599520/1‑150 (MQ=255)

                                                                                                                      cACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGtt                        <  1:560138/150‑1 (MQ=255)

                                                                                                                       aCCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTa                       <  2:1375659/150‑1 (MQ=255)

                                                                                                                        ccGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTAt                      <  1:3385920/150‑1 (MQ=255)

                                                                                                                           cTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTa                   <  1:1142296/150‑1 (MQ=255)

                                                                                                                             gTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAAt                 <  1:3711558/150‑1 (MQ=255)

                                                                                                                                 gcgcGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAg             >  1:2740042/1‑150 (MQ=255)

                                                                                                                                   gcgTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTa           <  1:3467382/150‑1 (MQ=255)

                                                                                                                                    cgTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGACTTTACGTTATGTCATTCAGTAt          >  1:2939590/1‑150 (MQ=255)

                                                                                                                                    cgTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTAt          <  1:2421287/150‑1 (MQ=255)

                                                                                                                                      ttATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCa        >  2:3139612/1‑150 (MQ=255)

                                                                                                                                      ttATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCa        <  1:733454/150‑1 (MQ=255)

                                                                                                                                      ttATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCa        >  1:117571/1‑150 (MQ=255)

                                                                                                                                       tATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCAc       <  1:616084/150‑1 (MQ=255)

                                                                                                                                          cGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGcc    <  1:2461214/150‑1 (MQ=255)

                                                                                                                                           gttgttGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGccc   >  2:2467655/1‑150 (MQ=255)

                                                                                                                                           gttgttGTAGCACCCTGCCAGCGCCAGCATGAGCATGCAGGCGGCTACGCGTAACTTTTTCATTGTTCCTCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGccc   <  2:2786911/150‑1 (MQ=255)

                                                                                                                                            ttgttgTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCa  <  2:1265615/150‑1 (MQ=255)

TCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCA  >  CP009273/2645445‑2645734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCA  >  CP009273/2645443‑2645734

TGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTA                                                                                                                                                >  SRR19973061.435897/1‑150 (MQ=60)

 GTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAG                                                                                                                                               >  SRR19973061.1268740/1‑150 (MQ=60)

     GCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACC                                                                                                                                           >  SRR19973061.2880365/1‑150 (MQ=60)

       ACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCT                                                                                                                                         >  SRR19973061.2255314/1‑150 (MQ=60)

       ACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCT                                                                                                                                         >  SRR19973061.3689437/1‑150 (MQ=60)

        CCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTG                                                                                                                                        >  SRR19973061.3111191/1‑150 (MQ=60)

         CTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTCACTTCAGAAAGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGC                                                                                                                                       >  SRR19973061.1965445/1‑150 (MQ=60)

         CTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGC                                                                                                                                       >  SRR19973061.1965589/1‑150 (MQ=60)

         CTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGC                                                                                                                                       <  SRR19973061.90858/150‑1 (MQ=60)

         CTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGC                                                                                                                                       <  SRR19973061.90756/150‑1 (MQ=60)

         CTTACCGGCACTCTGTTGGGCCAGGTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGC                                                                                                                                       >  SRR19973061.2794077/1‑150 (MQ=60)

           TACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCA                                                                                                                                     <  SRR19973061.2252741/150‑1 (MQ=60)

              CGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCG                                                                                                                                  >  SRR19973061.2633919/1‑150 (MQ=60)

                 CACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCA                                                                                                                               <  SRR19973061.187353/150‑1 (MQ=60)

                     CTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCAT                                                                                                                           <  SRR19973061.2911177/150‑1 (MQ=60)

                     CTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCAT                                                                                                                           <  SRR19973061.2911949/150‑1 (MQ=60)

                        TTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAG                                                                                                                        <  SRR19973061.3205007/150‑1 (MQ=60)

                        TTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAG                                                                                                                        <  SRR19973061.3170842/150‑1 (MQ=60)

                        TTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAG                                                                                                                        <  SRR19973061.3170760/150‑1 (MQ=60)

                          GGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCA                                                                                                                      >  SRR19973061.3162293/1‑150 (MQ=60)

                          GGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCA                                                                                                                      >  SRR19973061.3162267/1‑150 (MQ=60)

                            GCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATG                                                                                                                    >  SRR19973061.876124/1‑150 (MQ=60)

                            GCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATG                                                                                                                    >  SRR19973061.3287707/1‑150 (MQ=60)

                             CCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGC                                                                                                                   >  SRR19973061.2111897/1‑150 (MQ=60)

                               AGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAGGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCCGCATTAGCATGCAG                                                                                                                 >  SRR19973061.3189255/1‑150 (MQ=60)

                               AGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAGGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAG                                                                                                                 >  SRR19973061.3196219/1‑150 (MQ=60)

                               AGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAG                                                                                                                 >  SRR19973061.2282609/1‑150 (MQ=60)

                               AGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCA‑TAGCATGCAGG                                                                                                                >  SRR19973061.3189254/1‑150 (MQ=60)

                                  TTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCG                                                                                                              >  SRR19973061.1615992/1‑150 (MQ=60)

                                   TTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGG                                                                                                             >  SRR19973061.3594160/1‑150 (MQ=60)

                                   TTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATCAGCATGCAGGCGG                                                                                                             >  SRR19973061.3372231/1‑150 (MQ=60)

                                     TGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCT                                                                                                           >  SRR19973061.1574496/1‑150 (MQ=60)

                                      GTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTA                                                                                                          >  SRR19973061.3274826/1‑150 (MQ=60)

                                       TCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTAC                                                                                                         >  SRR19973061.3026376/1‑150 (MQ=60)

                                       TCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTGTCTGAAGAGGCCGCTTGAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTAC                                                                                                         <  SRR19973061.2116171/150‑1 (MQ=60)

                                         TCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTCCGC                                                                                                       >  SRR19973061.2770806/1‑150 (MQ=60)

                                         TCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGC                                                                                                       >  SRR19973061.3553918/1‑150 (MQ=60)

                                         TCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGC                                                                                                       >  SRR19973061.3552873/1‑150 (MQ=60)

                                          CTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCG                                                                                                      <  SRR19973061.1916720/150‑1 (MQ=60)

                                           TCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGT                                                                                                     <  SRR19973061.1443501/150‑1 (MQ=60)

                                            CCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTA                                                                                                    <  SRR19973061.3370026/150‑1 (MQ=60)

                                             CGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAA                                                                                                   <  SRR19973061.2486518/150‑1 (MQ=60)

                                             CGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAA                                                                                                   <  SRR19973061.577004/150‑1 (MQ=60)

                                             CGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAA                                                                                                   <  SRR19973061.3098/150‑1 (MQ=60)

                                             CGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAA                                                                                                   <  SRR19973061.3344647/150‑1 (MQ=60)

                                             CGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAA                                                                                                   >  SRR19973061.817390/1‑150 (MQ=60)

                                             CGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAA                                                                                                   >  SRR19973061.2219078/1‑150 (MQ=60)

                                             CGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAA                                                                                                   >  SRR19973061.2219037/1‑150 (MQ=60)

                                                   GGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTT                                                                                             >  SRR19973061.354994/1‑150 (MQ=60)

                                                   GGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTT                                                                                             >  SRR19973061.355053/1‑150 (MQ=60)

                                                   GGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTT                                                                                             >  SRR19973061.187708/1‑150 (MQ=60)

                                                    GAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTC                                                                                            >  SRR19973061.1311926/1‑150 (MQ=60)

                                                      GAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCAT                                                                                          <  SRR19973061.1906056/150‑1 (MQ=60)

                                                       AGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATT                                                                                         <  SRR19973061.340989/150‑1 (MQ=60)

                                                       AGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATT                                                                                         >  SRR19973061.2919644/1‑150 (MQ=60)

                                                         TTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTT                                                                                       <  SRR19973061.3561799/150‑1 (MQ=60)

                                                          TTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTT                                                                                      <  SRR19973061.3281529/150‑1 (MQ=60)

                                                          TTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTT                                                                                      <  SRR19973061.184093/150‑1 (MQ=60)

                                                          TTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTT                                                                                      <  SRR19973061.665691/150‑1 (MQ=60)

                                                          TTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTT                                                                                      <  SRR19973061.666803/150‑1 (MQ=60)

                                                              CTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATC                                                                                  >  SRR19973061.2721219/1‑150 (MQ=60)

                                                                 AACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCT                                                                               <  SRR19973061.3333293/150‑1 (MQ=60)

                                                                       CGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACA                                                                         <  SRR19973061.1218642/150‑1 (MQ=60)

                                                                       CGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACA                                                                         <  SRR19973061.1218608/150‑1 (MQ=60)

                                                                           TTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGG                                                                     <  SRR19973061.1159965/150‑1 (MQ=60)

                                                                             CTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCC                                                                   <  SRR19973061.3057524/150‑1 (MQ=60)

                                                                                 AGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTT                                                               <  SRR19973061.1029603/150‑1 (MQ=60)

                                                                                  GAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTA                                                              <  SRR19973061.2282689/150‑1 (MQ=60)

                                                                                      CCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAA                                                          >  SRR19973061.1679159/1‑150 (MQ=60)

                                                                                       CGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAAC                                                         >  SRR19973061.2119955/1‑150 (MQ=60)

                                                                                       CGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAAC                                                         <  SRR19973061.1087753/150‑1 (MQ=60)

                                                                                       CGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATGAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAAC                                                         <  SRR19973061.3215689/150‑1 (MQ=60)

                                                                                        GCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACG                                                        >  SRR19973061.1187194/1‑150 (MQ=60)

                                                                                        GCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACG                                                        >  SRR19973061.1185276/1‑150 (MQ=60)

                                                                                         CTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGT                                                       >  SRR19973061.706916/1‑150 (MQ=60)

                                                                                          TTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTC                                                      <  SRR19973061.2429897/150‑1 (MQ=60)

                                                                                          TTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTC                                                      <  SRR19973061.2133687/150‑1 (MQ=60)

                                                                                           TTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCG                                                     <  SRR19973061.2834852/150‑1 (MQ=60)

                                                                                            TAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGA                                                    >  SRR19973061.831797/1‑150 (MQ=60)

                                                                                              GTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCagggatgaaaaatgtaaa                                                  >  SRR19973061.1934482/1‑132 (MQ=60)

                                                                                                AACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAA                                                <  SRR19973061.1721018/150‑1 (MQ=60)

                                                                                                AACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAA                                                >  SRR19973061.3562773/1‑150 (MQ=60)

                                                                                                     CAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATT                                           <  SRR19973061.1799983/150‑1 (MQ=60)

                                                                                                     CAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATT                                           <  SRR19973061.3490768/150‑1 (MQ=60)

                                                                                                     CAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATT                                           <  SRR19973061.3515955/150‑1 (MQ=60)

                                                                                                        AAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATG                                        >  SRR19973061.2750680/1‑150 (MQ=60)

                                                                                                         AGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCGCCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGC                                       >  SRR19973061.3051793/1‑150 (MQ=60)

                                                                                                         AGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGC                                       <  SRR19973061.2166822/150‑1 (MQ=60)

                                                                                                          GGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCG                                      <  SRR19973061.3063544/150‑1 (MQ=60)

                                                                                                            CGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTG                                    >  SRR19973061.2841567/1‑150 (MQ=60)

                                                                                                               ATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGGAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGATACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGA                                 <  SRR19973061.868756/150‑1 (MQ=60)

                                                                                                                        CACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTT                        >  SRR19973061.324351/1‑150 (MQ=60)

                                                                                                                        CACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTT                        <  SRR19973061.561608/150‑1 (MQ=60)

                                                                                                                        CACCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTT                        <  SRR19973061.561586/150‑1 (MQ=60)

                                                                                                                          CCGCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTAT                      <  SRR19973061.3394618/150‑1 (MQ=60)

                                                                                                                            GCTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGT                    >  SRR19973061.1531474/1‑150 (MQ=60)

                                                                                                                             CTGTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTA                   <  SRR19973061.1145201/150‑1 (MQ=60)

                                                                                                                               GTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAAT                 <  SRR19973061.3606339/150‑1 (MQ=60)

                                                                                                                               GTTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAAT                 <  SRR19973061.3721048/150‑1 (MQ=60)

                                                                                                                                TTGGCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATT                >  SRR19973061.1587542/1‑150 (MQ=60)

                                                                                                                                   GCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAG             >  SRR19973061.2747091/1‑150 (MQ=60)

                                                                                                                                   GCGCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAG             >  SRR19973061.2748367/1‑150 (MQ=60)

                                                                                                                                     GCGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTA           <  SRR19973061.3476260/150‑1 (MQ=60)

                                                                                                                                      CGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGACTTTACGTTATGTCATTCAGTAT          >  SRR19973061.2947153/1‑150 (MQ=60)

                                                                                                                                      CGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTCT          >  SRR19973061.2947313/1‑150 (MQ=60)

                                                                                                                                      CGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTAT          <  SRR19973061.3709460/150‑1 (MQ=60)

                                                                                                                                      CGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTAT          <  SRR19973061.2427467/150‑1 (MQ=60)

                                                                                                                                      CGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTAT          <  SRR19973061.1785990/150‑1 (MQ=60)

                                                                                                                                      CGTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCACCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTAT          <  SRR19973061.2080382/150‑1 (MQ=60)

                                                                                                                                       GTTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGTCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATC         >  SRR19973061.849581/1‑150 (MQ=60)

                                                                                                                                        TTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCA        >  SRR19973061.117958/1‑150 (MQ=60)

                                                                                                                                        TTATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCA        <  SRR19973061.735376/150‑1 (MQ=60)

                                                                                                                                         TATCGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCAC       <  SRR19973061.617665/150‑1 (MQ=60)

                                                                                                                                            CGTTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCC    <  SRR19973061.2467481/150‑1 (MQ=60)

                                                                                                                                              TTGTTGTAGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATATACGTTATGTAATTCAGTATCACGCCCA  >  SRR19973061.2195536/1‑150 (MQ=60)

TGTCAGCACCTTACCGGCACTCTGTTGGGCCAGTTTTTGTCTCTCCGGCACGGAGAGTTTTGCTGAACTCGCGTTTTCTGAAGAGGCCGCTTTAGTAACTTCAGAAGGCGCATCTTTTTTCACCGCTGTTGGCGCGTTATCGTTGTTGTCGCACCCTGCCAGCGCCAGCATTAGCATGCAGGCGGCTACGCGTAACTTTTTCATTTTTCATCCCTGCCACAATGGCCCGTTAGCAACGTCGAATAATTATTATGCGTGAGAATTTACGTTATGTAATTCAGTATCACGCCCA  >  CP009273/2645443‑2645734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`