Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,664,138 A→C 63.6% E130D (GAA→GAC)  hcaF → 3‑phenylpropionate dioxygenase, small (beta) subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,664,1380AC63.6% 300.6 / 188.3 203E130D (GAA→GAC) hcaF3‑phenylpropionate dioxygenase, small (beta) subunit
Reads supporting (aligned to +/- strand):  ref base A (36/38);  new base C (71/58);  total (107/96)
Fisher's exact test for biased strand distribution p-value = 3.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 207 total aligned reads displayed.

AAACGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATCGAATTTATGCG  >  CP009273/2663993‑2664284
                                                                                                                                                 |                                                                                                                                                  
aaaCGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGacac                                                                                                                                                  <  2:1539471/148‑1 (MQ=255)
aaaCGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAAc                                                                                                                                                  <  2:2056726/148‑1 (MQ=255)
  aCGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACAt                                                                                                                                                >  1:3258484/1‑148 (MQ=255)
   cGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACAtt                                                                                                                                               <  1:1950607/148‑1 (MQ=255)
      gCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTa                                                                                                                                            <  1:47169/148‑1 (MQ=255)
          ggCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGtt                                                                                                                                        >  1:954003/1‑148 (MQ=255)
             cTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGa                                                                                                                                     <  1:228281/148‑1 (MQ=255)
                ggCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATAGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAAcg                                                                                                                                  <  2:2223856/148‑1 (MQ=255)
                  cAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAAcgcg                                                                                                                                <  2:2209675/148‑1 (MQ=255)
                    gaagaGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGtt                                                                                                                              <  2:176515/148‑1 (MQ=255)
                    gaagaGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGtt                                                                                                                              >  2:483623/1‑148 (MQ=255)
                       gagCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCg                                                                                                                           >  2:1075047/1‑148 (MQ=255)
                             ccgTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCACACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAg                                                                                                                     <  2:3151364/148‑1 (MQ=255)
                             ccgTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAg                                                                                                                     <  1:3063361/148‑1 (MQ=255)
                             ccgTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAg                                                                                                                     <  2:2859909/148‑1 (MQ=255)
                                  aCGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGc                                                                                                                <  1:2799472/148‑1 (MQ=255)
                                  aCGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGc                                                                                                                <  1:1223103/148‑1 (MQ=255)
                                   cGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGcc                                                                                                               <  2:698555/148‑1 (MQ=255)
                                     cACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGt                                                                                                             <  2:653815/148‑1 (MQ=255)
                                      aCCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTc                                                                                                            <  2:568973/148‑1 (MQ=255)
                                      aCCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTc                                                                                                            <  2:2354508/148‑1 (MQ=255)
                                       cccGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCt                                                                                                           <  2:2268739/148‑1 (MQ=255)
                                          gTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGa                                                                                                        <  2:3022794/148‑1 (MQ=255)
                                           tCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGaa                                                                                                       >  2:852191/1‑148 (MQ=255)
                                            cACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAg                                                                                                      >  1:3225096/1‑148 (MQ=255)
                                              cTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGAt                                                                                                    <  1:2755597/148‑1 (MQ=255)
                                              cTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGAt                                                                                                    >  2:1038626/1‑148 (MQ=255)
                                              cTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGAt                                                                                                    <  1:1692410/148‑1 (MQ=255)
                                               ttAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATg                                                                                                   >  2:124011/1‑148 (MQ=255)
                                               ttAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATg                                                                                                   >  2:59982/1‑148 (MQ=255)
                                                  aTCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACa                                                                                                >  1:457665/1‑148 (MQ=255)
                                                  aTCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACa                                                                                                >  2:831470/1‑148 (MQ=255)
                                                   tCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACaa                                                                                               >  1:1534758/1‑148 (MQ=255)
                                                   tCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACaa                                                                                               >  1:908113/1‑148 (MQ=255)
                                                   tCAGCAACTGCCAGAGAAGCGAAACCGACATCCCACACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACaa                                                                                               <  2:2854772/148‑1 (MQ=255)
                                                    cAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAAc                                                                                              <  2:351354/148‑1 (MQ=255)
                                                     aGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACt                                                                                             >  1:876738/1‑148 (MQ=255)
                                                     aGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACt                                                                                             >  1:1081783/1‑148 (MQ=255)
                                                       cAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTgg                                                                                           >  1:1558602/1‑148 (MQ=255)
                                                         aCTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGgcg                                                                                         <  1:1327385/148‑1 (MQ=255)
                                                          cTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGgcgc                                                                                        >  2:2730440/1‑148 (MQ=255)
                                                          cTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGgcgc                                                                                        >  1:1587445/1‑148 (MQ=255)
                                                          cTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGgcgc                                                                                        >  2:706093/1‑148 (MQ=255)
                                                               aGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCt                                                                                   <  1:2526002/148‑1 (MQ=255)
                                                               aGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCt                                                                                   <  2:2806931/148‑1 (MQ=255)
                                                               aGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCt                                                                                   <  1:65726/148‑1 (MQ=255)
                                                                gATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTg                                                                                  >  2:189596/1‑148 (MQ=255)
                                                                gATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTg                                                                                  >  2:2072948/1‑148 (MQ=255)
                                                                  tAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGa                                                                                >  1:3437552/1‑148 (MQ=255)
                                                                  tAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGa                                                                                >  2:74467/1‑148 (MQ=255)
                                                                      cGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACgg                                                                            >  2:1477849/1‑148 (MQ=255)
                                                                        aaaCCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGa                                                                          >  1:776349/1‑148 (MQ=255)
                                                                         aaCCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGat                                                                         >  2:1432163/1‑148 (MQ=255)
                                                                         aaCCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGat                                                                         <  1:293304/148‑1 (MQ=255)
                                                                           ccGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGatat                                                                       >  2:2658885/1‑148 (MQ=255)
                                                                            cGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATc                                                                      >  1:896791/1‑148 (MQ=255)
                                                                               cATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATAGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTc                                                                   >  1:509260/1‑148 (MQ=255)
                                                                               cATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATAGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTc                                                                   <  1:837426/148‑1 (MQ=255)
                                                                                aTCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTcc                                                                  <  1:1276658/148‑1 (MQ=255)
                                                                                 tCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCt                                                                 >  1:2348827/1‑148 (MQ=255)
                                                                                 tCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGACACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCt                                                                 >  1:2931243/1‑148 (MQ=255)
                                                                                      aaCGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATc                                                            >  2:1362748/1‑148 (MQ=255)
                                                                                      aaCGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATc                                                            <  1:119319/148‑1 (MQ=255)
                                                                                       aCGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCa                                                           <  2:3258484/148‑1 (MQ=255)
                                                                                        cGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCaa                                                          >  2:1692474/1‑148 (MQ=255)
                                                                                        cGTATTTGCTGTGCGGGTAAATTATCTGCTTTATAGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCaa                                                          >  2:1826733/1‑148 (MQ=255)
                                                                                           aTTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCGTGCTGGAACGGGATATCGTCCTGGATCAAGCg                                                       <  1:1107978/148‑1 (MQ=255)
                                                                                               gCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTaa                                                   <  2:1074839/148‑1 (MQ=255)
                                                                                                 tgtgCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATc                                                 <  2:2342900/148‑1 (MQ=255)
                                                                                                  gtgCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCa                                                <  1:3428381/148‑1 (MQ=255)
                                                                                                        gTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTccc                                          >  2:1817405/1‑148 (MQ=255)
                                                                                                              tATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAAcc                                    >  1:2008803/1‑148 (MQ=255)
                                                                                                                 cTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGa                                 >  1:3502776/1‑148 (MQ=255)
                                                                                                                 cTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGa                                 <  1:965256/148‑1 (MQ=255)
                                                                                                                     tttATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAgtgt                             <  1:185486/148‑1 (MQ=255)
                                                                                                                      ttATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTa                            >  2:3354865/1‑148 (MQ=255)
                                                                                                                      ttATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTa                            >  2:2961190/1‑148 (MQ=255)
                                                                                                                      ttATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTa                            <  1:2112251/148‑1 (MQ=255)
                                                                                                                        aTCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACt                          >  1:2994648/1‑148 (MQ=255)
                                                                                                                          cGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGt                        >  1:606374/1‑148 (MQ=255)
                                                                                                                           gggCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGtt                       <  1:2518187/148‑1 (MQ=255)
                                                                                                                                caAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCtgat                  >  1:943287/1‑148 (MQ=255)
                                                                                                                                caAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCtgat                  <  2:2839854/148‑1 (MQ=255)
                                                                                                                                  aaaaGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCtgatga                >  1:1497305/1‑148 (MQ=255)
                                                                                                                                  aaaaGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCtgatga                >  1:1498133/1‑148 (MQ=255)
                                                                                                                                   aaaGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGaa               >  2:3294871/1‑148 (MQ=255)
                                                                                                                                    aaGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAAt              <  2:832727/148‑1 (MQ=255)
                                                                                                                                     agagCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATc             >  1:491185/1‑148 (MQ=255)
                                                                                                                                     agagCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATc             >  1:178363/1‑148 (MQ=255)
                                                                                                                                     agagCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATAACTTCCCATAACCTGAGTGTACTGTTCTGATGAATc             >  1:2696845/1‑148 (MQ=255)
                                                                                                                                       agCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATCGa           >  2:1052707/1‑148 (MQ=255)
                                                                                                                                       agCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATCGa           >  2:1958894/1‑148 (MQ=255)
                                                                                                                                         cgcgATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATCGAAt         <  1:2426931/148‑1 (MQ=255)
                                                                                                                                         cgcgATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATCGAAt         <  1:2426802/148‑1 (MQ=255)
                                                                                                                                          gcgATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGACGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATCGAAtt        <  1:222037/148‑1 (MQ=255)
                                                                                                                                          gcgATGAAACATTCTCTGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATCGAAtt        <  2:2529501/148‑1 (MQ=255)
                                                                                                                                            gATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATCGAATTTa      >  2:3098332/1‑148 (MQ=255)
                                                                                                                                            gATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATCGAATTTa      <  1:2903523/148‑1 (MQ=255)
                                                                                                                                             aTGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATCGAATTTAt     <  1:2003983/148‑1 (MQ=255)
                                                                                                                                                aaaCATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATCGAATTTATGCg  <  1:671359/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
AAACGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATCGAATTTATGCG  >  CP009273/2663993‑2664284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGGAAACGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATCGAATTTATGCGTG  >  CP009273/2663990‑2664286
                                                                                                                                                    |                                                                                                                                                    
TGGAAACGGGCTTGGCCTGGGCAGAAGAGCCGCCGTCACTCACCCGTCACTTAATCATCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACA                                                                                                                                                     <  SRR19973067.2533105/150‑1 (MQ=60)
TGGAAACGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACA                                                                                                                                                     <  SRR19973067.235866/150‑1 (MQ=60)
TGGAAACGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACA                                                                                                                                                     <  SRR19973067.2533012/150‑1 (MQ=60)
 GGAAACGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACAC                                                                                                                                                    <  SRR19973067.2417278/150‑1 (MQ=60)
   AAACGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACAT                                                                                                                                                  <  SRR19973067.3413868/150‑1 (MQ=60)
   AAACGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACAT                                                                                                                                                  >  SRR19973067.3278846/1‑150 (MQ=60)
    AACGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATT                                                                                                                                                 <  SRR19973067.922635/150‑1 (MQ=60)
      CGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCT                                                                                                                                               <  SRR19973067.1963887/150‑1 (MQ=60)
         GCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATG                                                                                                                                            <  SRR19973067.47804/150‑1 (MQ=60)
           ATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTT                                                                                                                                          >  SRR19973067.960926/1‑150 (MQ=60)
           ATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTT                                                                                                                                          >  SRR19973067.960536/1‑150 (MQ=60)
                CTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAAC                                                                                                                                     <  SRR19973067.229923/150‑1 (MQ=60)
                        AAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCG                                                                                                                             >  SRR19973067.2476855/1‑150 (MQ=60)
                         AGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGA                                                                                                                            >  SRR19973067.461262/1‑150 (MQ=60)
                          GAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGAC                                                                                                                           >  SRR19973067.2829718/1‑150 (MQ=60)
                          GAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGAC                                                                                                                           >  SRR19973067.3067008/1‑150 (MQ=60)
                                CCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTT                                                                                                                     <  SRR19973067.3083242/150‑1 (MQ=60)
                                CCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTT                                                                                                                     <  SRR19973067.3082861/150‑1 (MQ=60)
                                 CGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTC                                                                                                                    <  SRR19973067.512375/150‑1 (MQ=60)
                                   TCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGC                                                                                                                  >  SRR19973067.1101051/1‑150 (MQ=60)
                                     ACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCG                                                                                                                <  SRR19973067.2817531/150‑1 (MQ=60)
                                     ACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCG                                                                                                                <  SRR19973067.1231251/150‑1 (MQ=60)
                                       GCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTC                                                                                                              >  SRR19973067.1467444/1‑150 (MQ=60)
                                            CGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAA                                                                                                         <  SRR19973067.1278642/150‑1 (MQ=60)
                                            CGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAA                                                                                                         <  SRR19973067.2766143/150‑1 (MQ=60)
                                             GTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAG                                                                                                        >  SRR19973067.3245357/1‑150 (MQ=60)
                                              TCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGA                                                                                                       >  SRR19973067.1858037/1‑150 (MQ=60)
                                                 CTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGA                                                                                                    <  SRR19973067.2773404/150‑1 (MQ=60)
                                                 CTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGA                                                                                                    <  SRR19973067.1704062/150‑1 (MQ=60)
                                                   TAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACA                                                                                                  >  SRR19973067.460669/1‑150 (MQ=60)
                                                   TAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACA                                                                                                  >  SRR19973067.460834/1‑150 (MQ=60)
                                                    AATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAA                                                                                                 >  SRR19973067.914129/1‑150 (MQ=60)
                                                    AATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAA                                                                                                 >  SRR19973067.1545143/1‑150 (MQ=60)
                                                    AATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAA                                                                                                 >  SRR19973067.913745/1‑150 (MQ=60)
                                                      TCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACT                                                                                               >  SRR19973067.2673942/1‑150 (MQ=60)
                                                      TCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACT                                                                                               >  SRR19973067.1089183/1‑150 (MQ=60)
                                                      TCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACT                                                                                               >  SRR19973067.882592/1‑150 (MQ=60)
                                                      TCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACT                                                                                               >  SRR19973067.882559/1‑150 (MQ=60)
                                                        AGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGG                                                                                             >  SRR19973067.1569231/1‑150 (MQ=60)
                                                          CAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCG                                                                                           <  SRR19973067.811461/150‑1 (MQ=60)
                                                           AACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGC                                                                                          >  SRR19973067.1598320/1‑150 (MQ=60)
                                                            ACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCT                                                                                         <  SRR19973067.1336442/150‑1 (MQ=60)
                                                             CTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTT                                                                                        <  SRR19973067.151285/150‑1 (MQ=60)
                                                                  AGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGG                                                                                   <  SRR19973067.325008/150‑1 (MQ=60)
                                                                  AGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGG                                                                                   >  SRR19973067.2367270/1‑150 (MQ=60)
                                                                  AGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGG                                                                                   <  SRR19973067.2541035/150‑1 (MQ=60)
                                                                  AGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGG                                                                                   <  SRR19973067.2542558/150‑1 (MQ=60)
                                                                  AGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGG                                                                                   <  SRR19973067.66508/150‑1 (MQ=60)
                                                                   GATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGA                                                                                  >  SRR19973067.3459440/1‑150 (MQ=60)
                                                                   GATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGA                                                                                  >  SRR19973067.229914/1‑150 (MQ=60)
                                                                         CGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGA                                                                            >  SRR19973067.781730/1‑150 (MQ=60)
                                                                            AACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATAT                                                                         <  SRR19973067.295207/150‑1 (MQ=60)
                                                                            AACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATAT                                                                         <  SRR19973067.295188/150‑1 (MQ=60)
                                                                             ACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATC                                                                        >  SRR19973067.902711/1‑150 (MQ=60)
                                                                             ACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATC                                                                        >  SRR19973067.902710/1‑150 (MQ=60)
                                                                              CCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCG                                                                       <  SRR19973067.1556639/150‑1 (MQ=60)
                                                                               CGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGT                                                                      >  SRR19973067.647629/1‑150 (MQ=60)
                                                                                GACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATAGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTC                                                                     >  SRR19973067.512731/1‑150 (MQ=60)
                                                                                  CATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCT                                                                   >  SRR19973067.2364165/1‑150 (MQ=60)
                                                                                  CATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGACACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCT                                                                   >  SRR19973067.2949859/1‑150 (MQ=60)
                                                                                  CATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGACACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCT                                                                   >  SRR19973067.2949887/1‑150 (MQ=60)
                                                                                  CATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATAGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCT                                                                   <  SRR19973067.843116/150‑1 (MQ=60)
                                                                                   ATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTG                                                                  <  SRR19973067.1285350/150‑1 (MQ=60)
                                                                                         AACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAA                                                            <  SRR19973067.120449/150‑1 (MQ=60)
                                                                                           CGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGC                                                          <  SRR19973067.1579813/150‑1 (MQ=60)
                                                                                              ATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGT                                                       <  SRR19973067.1714928/150‑1 (MQ=60)
                                                                                              ATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCGTGCTGGAACGGGATATCGTCCTGGATCAAGCGGT                                                       <  SRR19973067.1115502/150‑1 (MQ=60)
                                                                                                     GTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGGTGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACT                                                <  SRR19973067.280423/150‑1 (MQ=60)
                                                                                                     GTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACT                                                <  SRR19973067.3450197/150‑1 (MQ=60)
                                                                                                     GTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACT                                                >  SRR19973067.727011/1‑150 (MQ=60)
                                                                                                        CGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCC                                             >  SRR19973067.3457686/1‑150 (MQ=60)
                                                                                                          GGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCA                                           <  SRR19973067.1163679/150‑1 (MQ=60)
                                                                                                          GGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCA                                           <  SRR19973067.2948055/150‑1 (MQ=60)
                                                                                                             AAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAA                                        >  SRR19973067.2357600/1‑150 (MQ=60)
                                                                                                               ATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACC                                      >  SRR19973067.2022150/1‑150 (MQ=60)
                                                                                                               ATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACC                                      >  SRR19973067.2022328/1‑150 (MQ=60)
                                                                                                                 TATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTG                                    >  SRR19973067.61870/1‑150 (MQ=60)
                                                                                                                 TATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTG                                    >  SRR19973067.1688228/1‑150 (MQ=60)
                                                                                                                  ATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGA                                   >  SRR19973067.3524967/1‑150 (MQ=60)
                                                                                                                  ATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGA                                   >  SRR19973067.3524977/1‑150 (MQ=60)
                                                                                                                    CTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGT                                 <  SRR19973067.971870/150‑1 (MQ=60)
                                                                                                                     TGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTG                                >  SRR19973067.2151844/1‑150 (MQ=60)
                                                                                                                        TTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTAC                             <  SRR19973067.186943/150‑1 (MQ=60)
                                                                                                                         TTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACT                            >  SRR19973067.2940680/1‑150 (MQ=60)
                                                                                                                         TTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACT                            >  SRR19973067.3013629/1‑150 (MQ=60)
                                                                                                                         TTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACT                            <  SRR19973067.2126308/150‑1 (MQ=60)
                                                                                                                           ATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGT                          >  SRR19973067.610257/1‑150 (MQ=60)
                                                                                                                              GGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCT                       <  SRR19973067.2534697/150‑1 (MQ=60)
                                                                                                                               GGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTG                      <  SRR19973067.60713/150‑1 (MQ=60)
                                                                                                                               GGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTG                      <  SRR19973067.1225465/150‑1 (MQ=60)
                                                                                                                                 CACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGAT                    >  SRR19973067.1329130/1‑150 (MQ=60)
                                                                                                                                 CACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGAT                    >  SRR19973067.953044/1‑150 (MQ=60)
                                                                                                                                 CACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGAT                    >  SRR19973067.949721/1‑150 (MQ=60)
                                                                                                                                   CAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGA                  >  SRR19973067.1507177/1‑150 (MQ=60)
                                                                                                                                   CAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGA                  >  SRR19973067.1507999/1‑150 (MQ=60)
                                                                                                                                   CAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGA                  >  SRR19973067.1508007/1‑150 (MQ=60)
                                                                                                                                      AAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATC               >  SRR19973067.493157/1‑150 (MQ=60)
                                                                                                                                      AAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATC               >  SRR19973067.494562/1‑150 (MQ=60)
                                                                                                                                      AAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATC               >  SRR19973067.179787/1‑150 (MQ=60)
                                                                                                                                      AAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATAACTTCCCATAACCTGAGTGTACTGTTCTGATGAATC               >  SRR19973067.2714191/1‑150 (MQ=60)
                                                                                                                                           GCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATCGAATT          <  SRR19973067.2054818/150‑1 (MQ=60)
                                                                                                                                            CGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATCGAATTT         <  SRR19973067.2442724/150‑1 (MQ=60)
                                                                                                                                            CGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATCGAATTT         <  SRR19973067.2442747/150‑1 (MQ=60)
                                                                                                                                            CGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATCGAATTT         <  SRR19973067.2442853/150‑1 (MQ=60)
                                                                                                                                             GCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGACGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATCGAATTTA        <  SRR19973067.223657/150‑1 (MQ=60)
                                                                                                                                              CGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATCGAATTTAT       >  SRR19973067.1949647/1‑150 (MQ=60)
                                                                                                                                               GATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATCGAATTTATG      <  SRR19973067.200795/150‑1 (MQ=60)
                                                                                                                                               GATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATCGAATTTATG      <  SRR19973067.2009650/150‑1 (MQ=60)
                                                                                                                                               GATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATCGAATTTATG      <  SRR19973067.2922035/150‑1 (MQ=60)
                                                                                                                                                ATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATCGAATTTATGC     <  SRR19973067.2017483/150‑1 (MQ=60)
                                                                                                                                                  GACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATCGAATTTATGCGT   >  SRR19973067.1946772/1‑150 (MQ=60)
                                                                                                                                                  GACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATCGAATTTATGCGT   >  SRR19973067.1398516/1‑150 (MQ=60)
                                                                                                                                                  GACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATCGAATTTATGCGT   >  SRR19973067.135118/1‑150 (MQ=60)
                                                                                                                                                   ACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATCGAATTTATGCGTG  <  SRR19973067.857944/150‑1 (MQ=60)
                                                                                                                                                   ACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATCGAATTTATGCGTG  <  SRR19973067.486949/150‑1 (MQ=60)
                                                                                                                                                   AAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATCGAATTTATGCGTG  <  SRR19973067.675955/150‑1 (MQ=60)
                                                                                                                                                   AAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATCGAATTTATGCGTG  <  SRR19973067.675884/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                    
TGGAAACGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATCGAATTTATGCGTG  >  CP009273/2663990‑2664286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑