Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F534 I1 R1 1235 230.2 7611948 95.1% 7238962 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 2,664,138 A→C E130D (GAA→GAC)  hcaF → 3‑phenylpropionate dioxygenase, small (beta) subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,664,1380AC99.5% 755.6 / NA 191E130D (GAA→GAC) hcaF3‑phenylpropionate dioxygenase, small (beta) subunit
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base C (87/103);  total (87/104)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 195 total aligned reads displayed.

GGAAACGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATCGAATTTATGC  >  CP009273/2663991‑2664283
                                                                                                                                                   |                                                                                                                                                 
ggAAACGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGacac                                                                                                                                                 >  2:968810/1‑150 (MQ=255)
 gAAACGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAcaca                                                                                                                                                <  2:3789593/150‑1 (MQ=255)
  aaaCGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCTGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACAt                                                                                                                                               >  1:2539829/1‑150 (MQ=255)
  aaaCGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACAt                                                                                                                                               <  1:130253/150‑1 (MQ=255)
  aaaCGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACAt                                                                                                                                               >  1:2363786/1‑150 (MQ=255)
  aaaCGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACAt                                                                                                                                               >  1:694299/1‑150 (MQ=255)
  aaaCGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACAt                                                                                                                                               <  1:3574268/150‑1 (MQ=255)
   aaCGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACAta                                                                                                                                              <  1:3100229/150‑2 (MQ=255)
    aCGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTc                                                                                                                                             <  2:2938535/150‑1 (MQ=255)
     cGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCt                                                                                                                                            <  1:1599675/150‑1 (MQ=255)
           tGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTg                                                                                                                                      <  2:706964/150‑1 (MQ=255)
               cTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAAc                                                                                                                                  >  2:2730469/1‑150 (MQ=255)
               cTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAAc                                                                                                                                  >  1:1757194/1‑150 (MQ=255)
               cTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAAc                                                                                                                                  >  2:826133/1‑150 (MQ=255)
                  ggCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAAcgcg                                                                                                                               <  1:767582/150‑1 (MQ=255)
                  ggCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAAcgcg                                                                                                                               <  1:2283155/150‑1 (MQ=255)
                    cAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGtt                                                                                                                             <  1:317679/150‑1 (MQ=255)
                     agaagaGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCCCAAAAAGAGCGCGATGACACATTCTAGGTTGGAACGCGttt                                                                                                                            >  2:1250640/1‑150 (MQ=255)
                     agaagaGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGttt                                                                                                                            >  2:296999/1‑150 (MQ=255)
                     agaagaGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGttt                                                                                                                            >  2:914098/1‑150 (MQ=255)
                      gaagaGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTc                                                                                                                           <  1:3538327/150‑1 (MQ=255)
                       aagaGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCg                                                                                                                          <  1:301818/150‑1 (MQ=255)
                          agCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACa                                                                                                                       >  1:1030109/1‑150 (MQ=255)
                            ccgccgTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACaaa                                                                                                                     <  1:1008176/150‑1 (MQ=255)
                                cgTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTc                                                                                                                 <  2:1404148/150‑1 (MQ=255)
                                 gTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCg                                                                                                                <  2:3620506/150‑1 (MQ=255)
                                   cACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGcc                                                                                                              <  2:3208580/150‑1 (MQ=255)
                                    aatcACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTAATATCTGTGCTGGTAAATTATCTGCTATAACGGGCACAAACAGAGCGCGATGACACATTCTATGTAGGAACGCGTTTCGACAAAGTTAGCCg                                                                                                             >  2:1972017/4‑150 (MQ=255)
                                      gCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTc                                                                                                           >  1:682887/1‑150 (MQ=255)
                                      gCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTc                                                                                                           >  1:2446125/1‑150 (MQ=255)
                                       cACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCt                                                                                                          <  2:3443772/150‑1 (MQ=255)
                                        aCCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTg                                                                                                         <  1:1378733/150‑1 (MQ=255)
                                        aCCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTg                                                                                                         >  1:1827554/1‑150 (MQ=255)
                                        aCCAGTCACTTAATCAGCAACTGCCAGAAAAGCGAAACAGACATCCCAAACGTATTTGCAGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCGATGTTGTAACTAGTATCTACAAAGTTCGCCTTCTg                                                                                                         >  2:2358177/1‑150 (MQ=255)
                                             tCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGa                                                                                                    <  1:1317598/150‑1 (MQ=255)
                                              cACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGAt                                                                                                   >  1:1587147/1‑150 (MQ=255)
                                                cTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGa                                                                                                 <  1:3420752/150‑1 (MQ=255)
                                                      cAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTg                                                                                           >  1:2510632/1‑150 (MQ=255)
                                                      cAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTg                                                                                           >  1:2510399/1‑150 (MQ=255)
                                                        gCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGgc                                                                                         >  2:524403/1‑150 (MQ=255)
                                                        gCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGgc                                                                                         >  1:3257275/1‑150 (MQ=255)
                                                         cAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGgcg                                                                                        >  2:2565771/1‑150 (MQ=255)
                                                             tGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTg                                                                                    >  1:1081994/1‑150 (MQ=255)
                                                               ccAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCt                                                                                  >  2:1288886/1‑150 (MQ=255)
                                                                 aGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTgg                                                                                >  2:2185253/1‑150 (MQ=255)
                                                                 aGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTgg                                                                                >  1:2614200/1‑150 (MQ=255)
                                                                   aTAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGaa                                                                              <  1:1836489/150‑1 (MQ=255)
                                                                      aGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACgg                                                                           >  2:2109365/1‑150 (MQ=255)
                                                                         gAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGat                                                                        >  2:103814/1‑150 (MQ=255)
                                                                          aaaCCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGata                                                                       >  1:205237/1‑150 (MQ=255)
                                                                           aaCCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGatat                                                                      <  1:968810/150‑1 (MQ=255)
                                                                            aCCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATc                                                                     <  2:2395628/150‑1 (MQ=255)
                                                                             ccGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCAGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCg                                                                    >  1:388339/1‑150 (MQ=255)
                                                                                aCATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTcc                                                                 <  2:3323061/150‑1 (MQ=255)
                                                                                 cNTCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCt                                                                >  1:494621/1‑150 (MQ=255)
                                                                                 cATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCt                                                                >  1:92513/1‑150 (MQ=255)
                                                                                  aTCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTg                                                               <  1:1708627/150‑1 (MQ=255)
                                                                                  aTCCCAAACGTATGTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAACAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTg                                                               <  2:3353147/150‑1 (MQ=255)
                                                                                   tCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTgg                                                              <  2:408385/150‑1 (MQ=255)
                                                                                   tCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTgg                                                              <  2:2698002/150‑1 (MQ=255)
                                                                                   tCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTgg                                                              <  2:2536299/150‑1 (MQ=255)
                                                                                   tCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTgg                                                              <  2:1655566/150‑1 (MQ=255)
                                                                                    cccAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGa                                                             <  1:3239042/150‑1 (MQ=255)
                                                                                     ccAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGAt                                                            >  2:3758268/1‑150 (MQ=255)
                                                                                         aCGTATTTGCTGTGCGGGTAAATTATCTGCTTTATTGGGAACAATAAGAGCGCGTTGAAACATTCTATGTTGGAACGCGTTTCGATTATGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGTAACGGGATATCGTCCTGGATCAAg                                                        <  1:272316/150‑1 (MQ=255)
                                                                                            tataTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCgg                                                     <  1:759884/150‑1 (MQ=255)
                                                                                              tttGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTa                                                   <  1:620616/150‑1 (MQ=255)
                                                                                                tGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAAt                                                 <  2:462888/150‑1 (MQ=255)
                                                                                                   tgtgCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCAc                                              >  2:3111301/1‑150 (MQ=255)
                                                                                                    gtgCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGACGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACt                                             >  2:3146195/1‑150 (MQ=255)
                                                                                                         ggTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCa                                        <  2:1774929/150‑1 (MQ=255)
                                                                                                         ggTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCa                                        <  2:3669664/150‑1 (MQ=255)
                                                                                                           tAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATa                                      <  1:2554902/150‑1 (MQ=255)
                                                                                                               ttATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCt                                  <  2:3187035/150‑1 (MQ=255)
                                                                                                                tATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTg                                 <  1:1041477/150‑1 (MQ=255)
                                                                                                                tATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTg                                 >  2:3217479/1‑150 (MQ=255)
                                                                                                                 aTCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGa                                <  1:296999/150‑1 (MQ=255)
                                                                                                                 aTCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGa                                >  1:2139022/1‑150 (MQ=255)
                                                                                                                    tGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAgtg                             >  1:36491/1‑150 (MQ=255)
                                                                                                                    tGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAgtg                             >  2:1626975/1‑150 (MQ=255)
                                                                                                                     gCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAgtgt                            >  2:439111/1‑150 (MQ=255)
                                                                                                                     gCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAgtgt                            >  2:612192/1‑150 (MQ=255)
                                                                                                                     gCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAgtgt                            <  2:1203190/150‑1 (MQ=255)
                                                                                                                      cTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTa                           <  1:2730469/150‑1 (MQ=255)
                                                                                                                      cTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTa                           <  1:826133/150‑1 (MQ=255)
                                                                                                                       tttATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGAGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTAc                          >  1:3087262/1‑150 (MQ=255)
                                                                                                                        ttATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACt                         >  2:3390280/1‑150 (MQ=255)
                                                                                                                            cGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTc                     <  1:1408163/150‑1 (MQ=255)
                                                                                                                                cacaAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCtgat                 >  1:2635775/1‑150 (MQ=255)
                                                                                                                                 acaAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCtgatg                <  2:73526/150‑1 (MQ=255)
                                                                                                                                  caAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCtgatga               <  2:3109620/150‑1 (MQ=255)
                                                                                                                                     aaaGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATc            >  1:2865229/1‑150 (MQ=255)
                                                                                                                                     aaaGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATc            >  1:649272/1‑150 (MQ=255)
                                                                                                                                     aaaGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATc            <  1:1613355/150‑1 (MQ=255)
                                                                                                                                     aaaGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATc            <  1:3278849/150‑1 (MQ=255)
                                                                                                                                      aaGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATCg           <  2:2151132/150‑1 (MQ=255)
                                                                                                                                        gagCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATCGaa         >  1:285455/1‑150 (MQ=255)
                                                                                                                                          gcgcGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATCGAAtt       <  2:682887/150‑1 (MQ=255)
                                                                                                                                           cgcgATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATCGAAttc      >  2:1316553/1‑149 (MQ=255)
                                                                                                                                             cgATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATCGAATTTAt    >  2:2707409/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
GGAAACGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATCGAATTTATGC  >  CP009273/2663991‑2664283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTGGAAACGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATCGAATTTATGCGTGT  >  CP009273/2663989‑2664287
                                                                                                                                                     |                                                                                                                                                     
CTGGAAACGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAC                                                                                                                                                       >  SRR19973061.1195326/1‑150 (MQ=60)
 TGGAAACGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACA                                                                                                                                                      >  SRR19973061.2861299/1‑150 (MQ=60)
 TGGAAACGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACA                                                                                                                                                      <  SRR19973061.587129/150‑1 (MQ=60)
  GGAAACGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACAC                                                                                                                                                     <  SRR19973061.3703677/150‑1 (MQ=60)
   GAAACGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACA                                                                                                                                                    >  SRR19973061.3199815/1‑150 (MQ=60)
    AAACGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCTGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACAT                                                                                                                                                   >  SRR19973061.2546293/1‑150 (MQ=60)
    AAACGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACAT                                                                                                                                                   <  SRR19973061.3583428/150‑1 (MQ=60)
    AAACGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACAT                                                                                                                                                   <  SRR19973061.130670/150‑1 (MQ=60)
    AAACGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACAT                                                                                                                                                   >  SRR19973061.2369796/1‑150 (MQ=60)
    AAACGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACAT                                                                                                                                                   >  SRR19973061.696104/1‑150 (MQ=60)
     AACGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATA                                                                                                                                                  <  SRR19973061.3108360/150‑1 (MQ=60)
     AACGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATA                                                                                                                                                  <  SRR19973061.3108217/150‑1 (MQ=60)
     AACGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATA                                                                                                                                                  <  SRR19973061.3104143/150‑1 (MQ=60)
       CGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCT                                                                                                                                                <  SRR19973061.1603649/150‑1 (MQ=60)
               GCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGA                                                                                                                                        <  SRR19973061.1311598/150‑1 (MQ=60)
               GCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGA                                                                                                                                        <  SRR19973061.989308/150‑1 (MQ=60)
                 CTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAAC                                                                                                                                      >  SRR19973061.1761596/1‑150 (MQ=60)
                 CTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAAC                                                                                                                                      >  SRR19973061.3251084/1‑150 (MQ=60)
                    GGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCG                                                                                                                                   <  SRR19973061.2288926/150‑1 (MQ=60)
                    GGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCG                                                                                                                                   <  SRR19973061.2288934/150‑1 (MQ=60)
                    GGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCG                                                                                                                                   <  SRR19973061.769597/150‑1 (MQ=60)
                      CAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTT                                                                                                                                 <  SRR19973061.318508/150‑1 (MQ=60)
                       AGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTT                                                                                                                                <  SRR19973061.286778/150‑1 (MQ=60)
                       AGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTT                                                                                                                                >  SRR19973061.464147/1‑150 (MQ=60)
                        GAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTC                                                                                                                               <  SRR19973061.3547390/150‑1 (MQ=60)
                         AAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCG                                                                                                                              <  SRR19973061.302617/150‑1 (MQ=60)
                           GAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTACATAATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGAC                                                                                                                            >  SRR19973061.2979517/1‑150 (MQ=60)
                            AGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACA                                                                                                                           >  SRR19973061.1032748/1‑150 (MQ=60)
                              CCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAA                                                                                                                         <  SRR19973061.1010744/150‑1 (MQ=60)
                                        GCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTC                                                                                                               >  SRR19973061.684658/1‑150 (MQ=60)
                                        GCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTC                                                                                                               >  SRR19973061.2452362/1‑150 (MQ=60)
                                         CACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCT                                                                                                              <  SRR19973061.663390/150‑1 (MQ=60)
                                          ACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTG                                                                                                             >  SRR19973061.3047187/1‑150 (MQ=60)
                                          ACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTG                                                                                                             <  SRR19973061.1382219/150‑1 (MQ=60)
                                          ACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTG                                                                                                             >  SRR19973061.1832135/1‑150 (MQ=60)
                                               TCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGA                                                                                                        <  SRR19973061.1320908/150‑1 (MQ=60)
                                                CACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGAT                                                                                                       >  SRR19973061.1591086/1‑150 (MQ=60)
                                                  CTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGA                                                                                                     <  SRR19973061.3429531/150‑1 (MQ=60)
                                                    TAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACA                                                                                                   >  SRR19973061.2541143/1‑150 (MQ=60)
                                                    TAATCAGCAACAGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACA                                                                                                   >  SRR19973061.1591303/1‑150 (MQ=60)
                                                      ATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAAC                                                                                                 >  SRR19973061.2076797/1‑150 (MQ=60)
                                                       TCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACGACT                                                                                                <  SRR19973061.2392484/150‑1 (MQ=60)
                                                       TCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACT                                                                                                >  SRR19973061.1724242/1‑150 (MQ=60)
                                                        CAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTG                                                                                               >  SRR19973061.2517026/1‑150 (MQ=60)
                                                        CAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTG                                                                                               <  SRR19973061.2622135/150‑1 (MQ=60)
                                                        CAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTG                                                                                               >  SRR19973061.2516793/1‑150 (MQ=60)
                                                          GCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGC                                                                                             >  SRR19973061.3265654/1‑150 (MQ=60)
                                                               TGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTG                                                                                        >  SRR19973061.1084767/1‑150 (MQ=60)
                                                                 CCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCT                                                                                      <  SRR19973061.1643934/150‑1 (MQ=60)
                                                                   AGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGG                                                                                    >  SRR19973061.2620912/1‑150 (MQ=60)
                                                                     ATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAA                                                                                  <  SRR19973061.1841087/150‑1 (MQ=60)
                                                                     ATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAA                                                                                  <  SRR19973061.1841071/150‑1 (MQ=60)
                                                                       AAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACG                                                                                >  SRR19973061.719285/1‑150 (MQ=60)
                                                                        AGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGG                                                                               <  SRR19973061.1576070/150‑1 (MQ=60)
                                                                        AGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGG                                                                               >  SRR19973061.2458782/1‑150 (MQ=60)
                                                                            AAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATA                                                                           >  SRR19973061.205850/1‑150 (MQ=60)
                                                                             AACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATAT                                                                          <  SRR19973061.971263/150‑1 (MQ=60)
                                                                               CCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCAGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCG                                                                        >  SRR19973061.389400/1‑150 (MQ=60)
                                                                                 GACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTC                                                                      <  SRR19973061.2693635/150‑1 (MQ=60)
                                                                                  ACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCC                                                                     >  SRR19973061.327972/1‑150 (MQ=60)
                                                                                   CNTCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCT                                                                    >  SRR19973061.495932/1‑150 (MQ=60)
                                                                                   CATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCT                                                                    >  SRR19973061.92826/1‑150 (MQ=60)
                                                                                    ATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTG                                                                   <  SRR19973061.1712918/150‑1 (MQ=60)
                                                                                      CCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGA                                                                 <  SRR19973061.3247377/150‑1 (MQ=60)
                                                                                      CCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGA                                                                 >  SRR19973061.1077285/1‑150 (MQ=60)
                                                                                           ACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATTGGGAACAATAAGAGCGCGTTGAAACATTCTATGTTGGAACGCGTTTCGATTATGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGTAACGGGATATCGTCCTGGATCAAG                                                            <  SRR19973061.273062/150‑1 (MQ=60)
                                                                                           ACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAG                                                            <  SRR19973061.273067/150‑1 (MQ=60)
                                                                                            CGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGC                                                           <  SRR19973061.2789811/150‑1 (MQ=60)
                                                                                              TATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGG                                                         <  SRR19973061.762193/150‑1 (MQ=60)
                                                                                              TATATGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGG                                                         <  SRR19973061.761878/150‑1 (MQ=60)
                                                                                                TTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTA                                                       <  SRR19973061.622200/150‑1 (MQ=60)
                                                                                                  TGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAATGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAAT                                                     <  SRR19973061.691993/150‑1 (MQ=60)
                                                                                                  TGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAAT                                                     >  SRR19973061.1259191/1‑150 (MQ=60)
                                                                                                  TGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAAT                                                     <  SRR19973061.3574170/150‑1 (MQ=60)
                                                                                                   GCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATC                                                    <  SRR19973061.951831/150‑1 (MQ=60)
                                                                                                     TGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCAC                                                  <  SRR19973061.916439/150‑1 (MQ=60)
                                                                                                            GTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCAT                                           >  SRR19973061.2265428/1‑150 (MQ=60)
                                                                                                             TAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATA                                          <  SRR19973061.2561097/150‑1 (MQ=60)
                                                                                                             TAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATA                                          <  SRR19973061.2561413/150‑1 (MQ=60)
                                                                                                               AATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAAC                                        <  SRR19973061.1337991/150‑1 (MQ=60)
                                                                                                               AATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAAC                                        >  SRR19973061.1408478/1‑150 (MQ=60)
                                                                                                                  TATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTG                                     <  SRR19973061.1044143/150‑1 (MQ=60)
                                                                                                                  TATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTG                                     <  SRR19973061.1044078/150‑1 (MQ=60)
                                                                                                                   ATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGA                                    <  SRR19973061.297791/150‑1 (MQ=60)
                                                                                                                   ATCTGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGA                                    >  SRR19973061.2144393/1‑150 (MQ=60)
                                                                                                                      TGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTG                                 >  SRR19973061.36628/1‑150 (MQ=60)
                                                                                                                      TGCTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTG                                 >  SRR19973061.2613243/1‑150 (MQ=60)
                                                                                                                        CTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTA                               <  SRR19973061.2737490/150‑1 (MQ=60)
                                                                                                                        CTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTA                               <  SRR19973061.828253/150‑1 (MQ=60)
                                                                                                                        CTTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTA                               <  SRR19973061.828288/150‑1 (MQ=60)
                                                                                                                         TTTATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGAGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTAC                              >  SRR19973061.3095222/1‑150 (MQ=60)
                                                                                                                            ATCGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTCCTGT                           >  SRR19973061.1918470/1‑150 (MQ=60)
                                                                                                                              CGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTC                         >  SRR19973061.2948606/1‑150 (MQ=60)
                                                                                                                              CGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTC                         <  SRR19973061.3315548/150‑1 (MQ=60)
                                                                                                                              CGGGCACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTC                         <  SRR19973061.1411713/150‑1 (MQ=60)
                                                                                                                                  CACAAAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGAT                     >  SRR19973061.2642545/1‑150 (MQ=60)
                                                                                                                                      AAAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAAT                 >  SRR19973061.1828107/1‑150 (MQ=60)
                                                                                                                                       AAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATC                >  SRR19973061.650937/1‑150 (MQ=60)
                                                                                                                                       AAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATC                >  SRR19973061.650855/1‑150 (MQ=60)
                                                                                                                                       AAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATC                >  SRR19973061.2872569/1‑150 (MQ=60)
                                                                                                                                       AAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATC                <  SRR19973061.3287293/150‑1 (MQ=60)
                                                                                                                                       AAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATC                <  SRR19973061.1617371/150‑1 (MQ=60)
                                                                                                                                       AAAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATC                <  SRR19973061.1617352/150‑1 (MQ=60)
                                                                                                                                        AAGAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCACCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATCG               >  SRR19973061.3462913/1‑150 (MQ=60)
                                                                                                                                          GAGCGCGATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATCGAA             >  SRR19973061.286220/1‑150 (MQ=60)
                                                                                                                                                 ATGACACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATCGAATTTATGC      >  SRR19973061.496001/1‑150 (MQ=60)
                                                                                                                                                     CACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATCGAATTTATGCGTGT  <  SRR19973061.3789835/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
CTGGAAACGGGCATGGCCTGGGCAGAAGAGCCGCCGTCACGCACCCGTCACTTAATCAGCAACTGCCAGATAAGCGAAACCGACATCCCAAACGTATTTGCTGTGCGGGTAAATTATCTGCTTTATCGGGCACAAAAAGAGCGCGATGAAACATTCTATGTTGGAACGCGTTTCGACAAAGTTCGCCGTCTGGAAGATGACAACTGGCGCTTGCTGGAACGGGATATCGTCCTGGATCAAGCGGTAATCACTTCCCATAACCTGAGTGTACTGTTCTGATGAATCGAATTTATGCGTGT  >  CP009273/2663989‑2664287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑