Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,820,170 C→A 35.7% I140I (ATC→ATA)  srlE → glucitol/sorbitol‑specific enzyme IIB component of PTS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,820,1700CA35.7% 311.8 / 183.7 204I140I (ATC→ATA) srlEglucitol/sorbitol‑specific enzyme IIB component of PTS
Reads supporting (aligned to +/- strand):  ref base C (48/83);  new base A (44/29);  total (92/112)
Fisher's exact test for biased strand distribution p-value = 1.29e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 207 total aligned reads displayed.

CTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTATTAAAAACCATTCTGCCGTTTATGGCATTCGTCTCGGCGCTCATTGGC  >  CP009273/2820023‑2820317
                                                                                                                                                   |                                                                                                                                                   
cTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATc                                                                                                                                                     >  1:3223797/1‑148 (MQ=255)
  cGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCAc                                                                                                                                                   <  2:704971/148‑1 (MQ=255)
  cGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATAAc                                                                                                                                                   <  2:503559/148‑1 (MQ=255)
     cGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATAACCGa                                                                                                                                                <  2:2727666/148‑1 (MQ=255)
      gggCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGaa                                                                                                                                               <  1:122279/148‑1 (MQ=255)
       ggCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAAc                                                                                                                                              <  2:1063088/148‑1 (MQ=255)
       ggCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAAc                                                                                                                                              <  2:2836968/148‑1 (MQ=255)
       ggCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAAc                                                                                                                                              <  2:2837112/148‑1 (MQ=255)
         cAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATAACCGAACaa                                                                                                                                            >  2:2981085/1‑148 (MQ=255)
          aaGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACaaa                                                                                                                                           <  2:2002776/148‑1 (MQ=255)
           aGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAg                                                                                                                                          <  1:1146977/148‑1 (MQ=255)
           aGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATAACCGAACAAAg                                                                                                                                          <  2:2556458/148‑1 (MQ=255)
            gtccggtccgCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGc                                                                                                                                         >  1:3270312/1‑148 (MQ=255)
            gtccggtccgCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATAACCGAACAAAGc                                                                                                                                         >  1:2217397/1‑148 (MQ=255)
             tccggtccgCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCg                                                                                                                                        <  2:2224256/148‑1 (MQ=255)
             tccggtccgCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATAACCGAACAAAGCg                                                                                                                                        <  2:1108576/148‑1 (MQ=255)
              ccggtccgCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATAACCGAACAAAGCGa                                                                                                                                       >  2:1652050/1‑148 (MQ=255)
                 gtccgCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATgg                                                                                                                                    <  2:2898794/148‑1 (MQ=255)
                  tccgCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGt                                                                                                                                   <  1:2079976/148‑1 (MQ=255)
                  tccgCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGt                                                                                                                                   >  1:1333155/1‑148 (MQ=255)
                  tccgCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATAACCGAACAAAGCGATGGt                                                                                                                                   >  1:1934451/1‑148 (MQ=255)
                      cTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATAACCGAACAAAGCGATGGTTTAc                                                                                                                               <  2:2939330/148‑1 (MQ=255)
                         gcgcAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATAACCGAACAAAGCGATGGTTTACTgg                                                                                                                            >  1:3342681/1‑148 (MQ=255)
                            cAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATAACCGAACAAAGCGATGGTTTACTGGCGa                                                                                                                         >  2:228472/1‑148 (MQ=255)
                                       ggAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATAACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATg                                                                                                              >  1:937697/1‑148 (MQ=255)
                                        gAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATgg                                                                                                             >  2:727901/1‑148 (MQ=255)
                                        gAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATgg                                                                                                             <  2:1158783/148‑1 (MQ=255)
                                         aaGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGACGGTGGGAATggg                                                                                                            >  2:348988/1‑148 (MQ=255)
                                              atTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATAACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATgg                                                                                                       >  1:3503817/1‑148 (MQ=255)
                                               tttATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATAACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATggg                                                                                                      <  2:1731171/148‑1 (MQ=255)
                                                  aTGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTc                                                                                                   <  1:680562/148‑1 (MQ=255)
                                                        ctGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGt                                                                                             >  2:360717/1‑148 (MQ=255)
                                                        ctGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATAACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGt                                                                                             >  1:3406698/1‑148 (MQ=255)
                                                             gTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCgg                                                                                        <  2:635766/148‑1 (MQ=255)
                                                             gTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATAACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCgg                                                                                        <  2:3081633/148‑1 (MQ=255)
                                                                aaaGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGc                                                                                     >  1:162109/1‑148 (MQ=255)
                                                                aaaGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATAACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGc                                                                                     >  2:310660/1‑148 (MQ=255)
                                                                   gaagaaAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGt                                                                                  <  2:545674/148‑1 (MQ=255)
                                                                   gaagaaAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATAACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGt                                                                                  >  2:1601592/1‑148 (MQ=255)
                                                                   gaagaaAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATAACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGt                                                                                  <  2:58052/148‑1 (MQ=255)
                                                                     agaaAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGttt                                                                                >  1:1449630/1‑148 (MQ=255)
                                                                      gaaAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTc                                                                               >  2:1803713/1‑148 (MQ=255)
                                                                      gaaAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATAACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTc                                                                               >  1:1073405/1‑148 (MQ=255)
                                                                       aaaaCATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCa                                                                              <  1:2133230/148‑1 (MQ=255)
                                                                           cATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGACTGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCt                                                                          >  2:1001762/1‑148 (MQ=255)
                                                                            aTCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTg                                                                         >  1:1096451/1‑148 (MQ=255)
                                                                            aTCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATAACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTg                                                                         <  2:1196883/148‑1 (MQ=255)
                                                                             tCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTgg                                                                        >  2:3045105/1‑148 (MQ=255)
                                                                               aCTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTc                                                                      <  2:2220273/148‑1 (MQ=255)
                                                                               aCTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTc                                                                      <  2:3332427/148‑1 (MQ=255)
                                                                                cTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCg                                                                     >  1:1496423/1‑148 (MQ=255)
                                                                                   tagtagGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATAACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGa                                                                  >  2:3223791/1‑148 (MQ=255)
                                                                                     gtagGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGaca                                                                <  2:3122386/148‑1 (MQ=255)
                                                                                     gtagGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATAACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGaca                                                                >  2:2193454/1‑148 (MQ=255)
                                                                                       agGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATAACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACAcc                                                              >  1:371153/1‑148 (MQ=255)
                                                                                        ggTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCa                                                             <  2:736312/148‑1 (MQ=255)
                                                                                            aTGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGa                                                         <  1:3444805/148‑1 (MQ=255)
                                                                                             tGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATAACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGac                                                        <  1:808482/148‑1 (MQ=255)
                                                                                               cGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGacac                                                      <  1:3131555/148‑1 (MQ=255)
                                                                                                 acacCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTg                                                    >  2:1816557/1‑148 (MQ=255)
                                                                                                 acacCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATAACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTg                                                    >  1:1943561/1‑148 (MQ=255)
                                                                                                    ccacAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTAt                                                 >  1:2702307/1‑148 (MQ=255)
                                                                                                     cacaACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTAtt                                                >  1:56150/1‑148 (MQ=255)
                                                                                                     cacaACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATAACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTAtt                                                >  2:2405230/1‑148 (MQ=255)
                                                                                                      acaACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTATTa                                               <  1:1722395/148‑1 (MQ=255)
                                                                                                             tctTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATAACCGAACACAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTATTAAAAACCa                                        <  1:2719645/148‑1 (MQ=255)
                                                                                                              ctTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTATTAAAAACCAt                                       <  2:3508004/148‑1 (MQ=255)
                                                                                                              ctTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTATTAAAAACCAt                                       <  1:3539825/148‑1 (MQ=255)
                                                                                                                  cGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTATTAAAAACCATTCTg                                   <  1:1421449/148‑1 (MQ=255)
                                                                                                                   gTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTATTAAAAACCATTCTGc                                  <  1:407623/148‑1 (MQ=255)
                                                                                                                   gTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTATTAAAAACCATTCTGc                                  <  1:2197171/148‑1 (MQ=255)
                                                                                                                    tGGGCCGTGACTATGACACCAGTAAGAAAATAACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTATTAAAAACCATTCTGcc                                 <  2:1461677/148‑1 (MQ=255)
                                                                                                                      ggCCGTGACTATGACACCAGTAAGAAAATAACCGAACAACGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTATTAAAAACCATTCTGCCGt                               <  1:2161266/148‑1 (MQ=255)
                                                                                                                        ccGTGACTATGACACCAGTAAGAAAATAACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTATTAAAAACCATTCTGCCGttt                             <  1:3441626/148‑1 (MQ=255)
                                                                                                                         cGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTATTAAAAACCATTCTGCCGTTTa                            <  1:2978676/148‑1 (MQ=255)
                                                                                                                           tGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTATTAAAAACCATTCTGCCGTTTATg                          <  2:461159/148‑1 (MQ=255)
                                                                                                                            gACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTATTAAAAACCATTCTGCCGTTTATgg                         >  1:1442163/1‑148 (MQ=255)
                                                                                                                            gACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTATTAAAAACCATTCTGCCGTTTATgg                         >  1:2863673/1‑148 (MQ=255)
                                                                                                                            gACTATGACACCAGTAAGAAAATAACCGAACAAAGCGATGGTTTACTGGCGAAGCTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCCTCGACACTGTATAAAAAACAAATATGCCGTGTAttg                         >  1:1575277/1‑146 (MQ=255)
                                                                                                                             aCTATGACACCAGTAAGAAAATAACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTATTAAAAACCATTCTGCCGTTTATGGc                        >  2:3390322/1‑148 (MQ=255)
                                                                                                                              cTATGACACCAGTAAGAAAATCACCGAACAACGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTATTAAAAACCATTCTGCCGTTTATGGCa                       <  2:1854148/148‑1 (MQ=255)
                                                                                                                              cTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTATTAAAAACCATTCTGCCGTTTATGGCa                       <  2:1697818/148‑1 (MQ=255)
                                                                                                                               tATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTATTAAAAACCATTCTGCCGTTTATGGCAt                      <  1:333806/148‑1 (MQ=255)
                                                                                                                                atcacacCAGTAAGAAAATAACCCAACAAAGCTATTGTTTACTGGCGATGGTGGGAATGGGCTTGGGGTCCACCGTTGCGGTGATTTTTCAATCTGGTCGTGACACAATCGACACTGTATTAAAAACCATTCTGCCGTTTATGGCAtt                     <  1:310651/145‑1 (MQ=255)
                                                                                                                                aTGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTATTAAAAACCATTCTGCCGTTTATGGCAtt                     >  2:3251895/1‑148 (MQ=255)
                                                                                                                                aTGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTATTAAAAACCATTCTGCCGTTTATGGCAtt                     >  1:42801/1‑148 (MQ=255)
                                                                                                                                   acacCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTATTAAAAACCATTCTGCCGTTTATGGCATTCGt                  >  1:965208/1‑148 (MQ=255)
                                                                                                                                     acCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTATTAAAAACCATTCTGCCGTTTATGGCATTCGtct                <  2:1706863/148‑1 (MQ=255)
                                                                                                                                        aGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTATTAAAAACCATTCTGCCGTTTATGGCATTCGTCTCgg             >  1:2398291/1‑148 (MQ=255)
                                                                                                                                         gTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTATTAAAAACCATTCTGCCGTTTATGGCATTCGTCTCGgc            >  1:3112981/1‑148 (MQ=255)
                                                                                                                                          tAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTATTAAAAACCATTCTGCCGTTTATGGCATTCGTCTCGgcg           >  1:1765773/1‑148 (MQ=255)
                                                                                                                                          tAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTATTAAAAACCATTCTGCCGTTTATGGCATTCGTCTCGgcg           >  1:2380588/1‑148 (MQ=255)
                                                                                                                                          tAAGAAAATAACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTATTAAAAACCATTCTGCCGTTTATGGCATTCGTCTCGgcg           >  2:2094299/1‑148 (MQ=255)
                                                                                                                                            aGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTATTAAAAACCATTCTGCCGTTTATGGCATTCGTCTCGGCGCt         <  2:1449630/148‑1 (MQ=255)
                                                                                                                                            aGAAAATAACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTATTAAAAACCATTCTGCCGTTTATGGCATTCGTCTCGGCGCt         >  1:1559367/1‑148 (MQ=255)
                                                                                                                                             gAAAATCACCGAACAACGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTATTAAAAACCATTCTGCCGTTTATGGCATTCGTCTCGGCGCTc        <  2:2594860/148‑1 (MQ=255)
                                                                                                                                             gAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTATTAAAAACCATTCTGCCGTTTATGGCATTCGTCTCGGCGCTc        <  1:1873172/148‑1 (MQ=255)
                                                                                                                                               aaaTAACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTATTAAAAACCATTCTGCCGTTTATGGCATTCGTCTCGGCGCTCAt      <  1:228472/148‑1 (MQ=255)
                                                                                                                                               aaaTAACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTATTAAAAACCATTCTGCCGTTTATGGCATTCGTCTCGGCGCTCAt      >  1:1117162/1‑148 (MQ=255)
                                                                                                                                                   cACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTATTAAAAACCATTCTGCCGTTTATGGCATTCGTCTCGGCGCTCATTGGc  <  1:3167783/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CTCGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTATTAAAAACCATTCTGCCGTTTATGGCATTCGTCTCGGCGCTCATTGGC  >  CP009273/2820023‑2820317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A