Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,820,170 C→A 20.9% I140I (ATC→ATA)  srlE → glucitol/sorbitol‑specific enzyme IIB component of PTS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,820,1700CA20.9% 497.3 / 83.6 186I140I (ATC→ATA) srlEglucitol/sorbitol‑specific enzyme IIB component of PTS
Reads supporting (aligned to +/- strand):  ref base C (74/73);  new base A (13/26);  total (87/99)
Fisher's exact test for biased strand distribution p-value = 7.14e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 187 total aligned reads displayed.

CGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTATTAAAAACCATTCTGCCGTTTATGGCATTCGTCTCGGCGCTCATTGGC  >  CP009273/2820025‑2820317
                                                                                                                                                 |                                                                                                                                                   
cGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCAc                                                                                                                                                   <  2:932187/148‑1 (MQ=255)
cGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCAc                                                                                                                                                   >  2:2591926/1‑148 (MQ=255)
      gCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACa                                                                                                                                             <  1:3600687/148‑1 (MQ=255)
       cAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACaa                                                                                                                                            <  2:3306805/148‑1 (MQ=255)
        aaGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATAACCGAACaaa                                                                                                                                           <  2:730298/148‑1 (MQ=255)
         aGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAg                                                                                                                                          >  2:1982625/1‑148 (MQ=255)
         aGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATAACCGAACAAAg                                                                                                                                          >  1:1914414/1‑148 (MQ=255)
               gtccgCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATgg                                                                                                                                    <  2:41232/148‑1 (MQ=255)
               gtccgCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATgg                                                                                                                                    <  2:41185/148‑1 (MQ=255)
               gtccgCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATgg                                                                                                                                    <  1:2537917/148‑1 (MQ=255)
                   gCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATAACCGAACAAAGCGATGGTTTa                                                                                                                                <  1:2185352/148‑1 (MQ=255)
                   gCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATAACCGAACAAAGCGATGGTTTa                                                                                                                                <  1:3187034/148‑1 (MQ=255)
                   gCTGGCGCAGTACATTGTGGAAGATATTTATGACTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTa                                                                                                                                <  1:2917311/148‑1 (MQ=255)
                    cTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTAc                                                                                                                               <  1:3379168/148‑1 (MQ=255)
                      ggCTCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTg                                                                                                                             <  2:239739/148‑1 (MQ=255)
                      ggCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTg                                                                                                                             <  2:2426713/148‑1 (MQ=255)
                       gcgcAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATAACCGAACAAAGCGATGGTTTACTgg                                                                                                                            >  1:2763632/1‑148 (MQ=255)
                            gTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAg                                                                                                                       >  1:4965/1‑148 (MQ=255)
                            gTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATAACCGAACAAAGCGATGGTTTACTGGCGAAg                                                                                                                       <  1:1130502/148‑1 (MQ=255)
                              aCATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGt                                                                                                                     <  1:597427/148‑1 (MQ=255)
                               cATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTg                                                                                                                    <  2:2520732/148‑1 (MQ=255)
                                aTTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTgg                                                                                                                   >  1:403962/1‑148 (MQ=255)
                                 ttGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTggg                                                                                                                  >  2:2263655/1‑148 (MQ=255)
                                  tgtgGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGa                                                                                                                 <  2:2301528/148‑1 (MQ=255)
                                  tgtgGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATAACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGa                                                                                                                 >  1:2263609/1‑148 (MQ=255)
                                   gtgGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATAACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGaa                                                                                                                <  2:900753/148‑1 (MQ=255)
                                      taatatatTTATTTCTCTGGAGTAAAAGAAGAAAACATCACTGTAGTAGGTGATTCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATAAACGAACAAAGCGATGGTTTACTGGCGAAGGTGGTAATgg                                                                                                             <  2:67780/144‑1 (MQ=255)
                                      gAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATgg                                                                                                             >  2:2383425/1‑148 (MQ=255)
                                      gAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATAACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATgg                                                                                                             <  2:1728169/148‑1 (MQ=255)
                                       aaGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATggg                                                                                                            >  1:82014/1‑148 (MQ=255)
                                        aGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATAACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGc                                                                                                           >  2:3792711/1‑148 (MQ=255)
                                         gATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCa                                                                                                          <  1:308040/148‑1 (MQ=255)
                                           tatTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATg                                                                                                        >  2:1331615/1‑148 (MQ=255)
                                           tatTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATg                                                                                                        <  1:2508343/148‑1 (MQ=255)
                                            atTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATgg                                                                                                       <  2:1010001/148‑1 (MQ=255)
                                              ttATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGACTGGGCATgggg                                                                                                     >  2:3642354/1‑148 (MQ=255)
                                              ttATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATgggg                                                                                                     <  2:2256456/148‑1 (MQ=255)
                                              ttATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATgggg                                                                                                     <  2:2907488/148‑1 (MQ=255)
                                                  gTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGACTGGGCATGGGGTCCa                                                                                                 >  2:2452955/1‑148 (MQ=255)
                                                  gTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCa                                                                                                 <  1:706640/148‑1 (MQ=255)
                                                  gTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATAACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCa                                                                                                 <  2:1678594/148‑1 (MQ=255)
                                                   tctcTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCAc                                                                                                >  2:462967/1‑148 (MQ=255)
                                                     tctGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATAACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCg                                                                                              <  1:1174185/148‑1 (MQ=255)
                                                         gCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGc                                                                                          >  2:2330555/1‑148 (MQ=255)
                                                              aaaGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGc                                                                                     >  2:3761858/1‑148 (MQ=255)
                                                              aaaGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGc                                                                                     >  2:26190/1‑148 (MQ=255)
                                                               aagaagAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCt                                                                                    >  2:1912178/1‑148 (MQ=255)
                                                                agaagaAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGACGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTg                                                                                   >  2:3804501/1‑148 (MQ=255)
                                                                 gaagaaAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGt                                                                                  >  2:980842/1‑148 (MQ=255)
                                                                 gaagaaAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGt                                                                                  <  1:3522910/148‑1 (MQ=255)
                                                                     aaaaCATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCa                                                                              >  1:2279200/1‑148 (MQ=255)
                                                                     aaaaCATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATAACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCa                                                                              >  1:2414370/1‑148 (MQ=255)
                                                                      aaaCATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCaa                                                                             >  2:3792917/1‑148 (MQ=255)
                                                                        aCATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATc                                                                           <  2:1471627/148‑1 (MQ=255)
                                                                        aCATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATc                                                                           <  2:244321/148‑1 (MQ=255)
                                                                         cATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCt                                                                          >  2:784993/1‑148 (MQ=255)
                                                                          aTCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTg                                                                         <  2:3790498/148‑1 (MQ=255)
                                                                          aTCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTg                                                                         <  1:3646739/148‑1 (MQ=255)
                                                                               tGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGt                                                                    >  1:2351569/1‑148 (MQ=255)
                                                                               tGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGt                                                                    <  1:3704940/148‑1 (MQ=255)
                                                                                 tagtagGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGa                                                                  >  1:2374936/1‑148 (MQ=255)
                                                                                  agtagGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGac                                                                 >  2:118338/1‑148 (MQ=255)
                                                                                    tagGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATAACCGAACACAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGacac                                                               <  1:1291562/148‑1 (MQ=255)
                                                                                     agGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACAcc                                                              <  1:3682655/148‑1 (MQ=255)
                                                                                       gTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATAACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCAt                                                            <  2:1915234/148‑1 (MQ=255)
                                                                                           tGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGac                                                        <  2:4965/148‑1 (MQ=255)
                                                                                            gCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGaca                                                       <  2:2832164/148‑1 (MQ=255)
                                                                                                 accacAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTa                                                  >  2:3478248/1‑148 (MQ=255)
                                                                                                 accacAACCCTCTTCCGTGGGCCGTGAATATGACACCAGTAAGAAAATAACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTa                                                  <  1:857010/148‑1 (MQ=255)
                                                                                                  ccacAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTAt                                                 <  1:390446/148‑1 (MQ=255)
                                                                                                    acaACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTATTa                                               >  2:661293/1‑148 (MQ=255)
                                                                                                    acaACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTATTa                                               <  2:2492717/148‑1 (MQ=255)
                                                                                                    acaACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTATTa                                               >  2:3510010/1‑148 (MQ=255)
                                                                                                     caACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTATTaa                                              <  1:3344099/148‑1 (MQ=255)
                                                                                                     caACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATAACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTATTaa                                              >  2:3404190/1‑148 (MQ=255)
                                                                                                     caACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATAACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTATTaa                                              >  2:3404210/1‑148 (MQ=255)
                                                                                                       aCCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTATTaaaa                                            <  2:2022306/148‑1 (MQ=255)
                                                                                                       aCCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTATTaaaa                                            <  2:2022404/148‑1 (MQ=255)
                                                                                                       aCCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTATTaaaa                                            >  2:1163983/1‑148 (MQ=255)
                                                                                                          ctctTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCCTGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTATTAAAAAcc                                         >  1:1814166/1‑148 (MQ=255)
                                                                                                          ctctTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTATTAAAAAcc                                         >  1:1814322/1‑148 (MQ=255)
                                                                                                           tctTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTATTAAAAACCa                                        <  1:2930280/148‑1 (MQ=255)
                                                                                                             ttCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTATTAAAAACCAtt                                      >  2:1141679/1‑148 (MQ=255)
                                                                                                                    ggCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTATTAAAAACCATTCTGCCGt                               >  1:1900171/1‑148 (MQ=255)
                                                                                                                    ggCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTATTAAAAACCATTCTGCCGt                               >  2:3151231/1‑148 (MQ=255)
                                                                                                                    ggCCGTGACTATGACACCAGTAAGAAAATAACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGGTTCAATCTGGTCGTGACACCATCGACACTGTATTAAAAACCATTCTGCCGt                               <  2:625425/148‑1 (MQ=255)
                                                                                                                      ccGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTATTAAAAACCATTCTGCCGttt                             <  1:1100416/148‑1 (MQ=255)
                                                                                                                       cGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTATTAAAAACCATTCTGCCGTTTa                            <  2:1214937/148‑1 (MQ=255)
                                                                                                                        gTGACTATGACACCAGTAAGAAAATAACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTATTAAAAACCATTCTGCCGTTTAt                           <  1:1862597/148‑1 (MQ=255)
                                                                                                                         tGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTATTAAAAACCATTCTGCCGTTTATg                          >  1:1521446/1‑148 (MQ=255)
                                                                                                                           aCTATGACACCAGTAAGAAAATAACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTATTAAAAACCATTCTGCCGTTTATGGc                        <  1:2407287/148‑1 (MQ=255)
                                                                                                                               tGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTATTAAAAACCATTCTGCCGTTTATGGCATTc                    <  1:360971/148‑1 (MQ=255)
                                                                                                                                   acCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTATTAAAAACCATTCTGCCGTTTATGGCATTCGtct                <  1:2607462/148‑1 (MQ=255)
                                                                                                                                          aGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTATTAAAAACCATTCTGCCGTTTATGGCATTCGTCTCGGCGCt         <  1:2906208/148‑1 (MQ=255)
                                                                                                                                           gAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTATTAAAAACCATTCTGCCGTTTATGGCATTCGTCTCGGCGCTc        >  2:3536447/1‑148 (MQ=255)
                                                                                                                                           gAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTATTAAAAACCATTCTGCCGTTTATGGCATTCGTCTCGGCGCTc        <  1:2849269/148‑1 (MQ=255)
                                                                                                                                            taaaTAACCGAACACTGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGTTCGTGACACCTTCGACACTGTATTAAAAACCATTCTGGCGTTTATGGCTTTCGTCTCGGCGCTCa       <  2:2723914/147‑1 (MQ=255)
                                                                                                                                            aaaaTAACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTATTAAAAACCATTCTGCCGTTTATGGCATTCGTCTCGGCGCTCa       >  1:931884/1‑148 (MQ=255)
                                                                                                                                                tCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTATTAAAAACCATTCTGCCGTTTATGGCATTCGTCTCGGCGCTCATTgg   >  2:896055/1‑148 (MQ=255)
                                                                                                                                                 cACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTATTAAAAACCATTCTGCCGTTTATGGCATTCGTCTCGGCGCTCATTGGc  >  1:3668140/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
CGACGGGCAAGTCCGGTCCGCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCACCGTTGCGGTGCTGTTTCAATCTGGTCGTGACACCATCGACACTGTATTAAAAACCATTCTGCCGTTTATGGCATTCGTCTCGGCGCTCATTGGC  >  CP009273/2820025‑2820317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A