Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,837,721 C→A 100% D131Y (GAC→TAC)  hycF ← formate hydrogenlyase complex iron‑sulfur protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,837,7210CA97.2% 554.2 / 6.2 178D131Y (GAC→TAC) hycFformate hydrogenlyase complex iron‑sulfur protein
Reads supporting (aligned to +/- strand):  ref base C (1/4);  new base A (72/101);  total (73/105)
Fisher's exact test for biased strand distribution p-value = 6.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 180 total aligned reads displayed.

GCCTCTTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCCGTCGGGCAGACTTC  >  CP009273/2837576‑2837865
                                                                                                                                                 |                                                                                                                                                
gCCTCTTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGa                                                                                                                                                <  1:520831/148‑1 (MQ=255)
 ccTCTTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTACCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGAt                                                                                                                                               >  1:2592929/1‑148 (MQ=255)
   tctTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGAtct                                                                                                                                             <  2:1630985/148‑1 (MQ=255)
   tctGTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGAtct                                                                                                                                             <  2:1818896/148‑1 (MQ=255)
     tttCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATctct                                                                                                                                           <  2:2156929/148‑1 (MQ=255)
     tttCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATctct                                                                                                                                           <  1:324287/148‑1 (MQ=255)
       tCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCttt                                                                                                                                         <  1:1130887/148‑1 (MQ=255)
       tCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCttt                                                                                                                                         <  2:2629355/148‑1 (MQ=255)
           atGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTgg                                                                                                                                     >  2:1492228/1‑148 (MQ=255)
            tGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGa                                                                                                                                    >  2:3274173/1‑148 (MQ=255)
            tGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGa                                                                                                                                    <  2:2571823/148‑1 (MQ=255)
            tGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGa                                                                                                                                    <  2:1973251/148‑1 (MQ=255)
            tGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGa                                                                                                                                    <  1:1950049/148‑1 (MQ=255)
               cGAGTCAGTTCAATACGGTCGGACGGCACCAGGAATTTCTGGCGCTTACAATCCTGGCAAGACTCAAAGCATTCGCGCTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCATCGCAATGGCGAAGTAGATCTCTTTCTGGACgg                                                                                                                                 >  2:1019875/1‑148 (MQ=255)
                  gTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGa                                                                                                                              >  1:2251181/1‑148 (MQ=255)
                       ttCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGa                                                                                                                         >  1:2193512/1‑148 (MQ=255)
                       ttCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGa                                                                                                                         >  1:2162029/1‑148 (MQ=255)
                         cAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACg                                                                                                                       <  2:2953390/148‑1 (MQ=255)
                         cAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACg                                                                                                                       <  2:687808/148‑1 (MQ=255)
                           aTACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGAt                                                                                                                     <  2:2187169/148‑1 (MQ=255)
                               ggTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCa                                                                                                                 >  2:883246/1‑148 (MQ=255)
                                          aCCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGt                                                                                                      <  2:144642/148‑1 (MQ=255)
                                            cAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTg                                                                                                    >  2:3479109/1‑148 (MQ=255)
                                            cAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTg                                                                                                    <  2:2162029/148‑1 (MQ=255)
                                            cAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGGAGGAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTg                                                                                                    >  1:344797/1‑148 (MQ=255)
                                               gCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGcac                                                                                                 >  2:93668/1‑148 (MQ=255)
                                               gCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGcac                                                                                                 >  2:1892345/1‑148 (MQ=255)
                                               gCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGcac                                                                                                 <  2:350958/148‑1 (MQ=255)
                                               gCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGcac                                                                                                 >  2:2876338/1‑148 (MQ=255)
                                                cATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGcaca                                                                                                >  1:1779094/1‑148 (MQ=255)
                                                 aTTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAg                                                                                               <  1:1047608/148‑1 (MQ=255)
                                                 aTTTCTGGCGCTTACATTCCGGGCAAGTCTAAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAg                                                                                               <  1:1920430/148‑1 (MQ=255)
                                                   ttCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAgcg                                                                                             <  1:1528310/148‑1 (MQ=255)
                                                   ttCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAgcg                                                                                             >  2:820220/1‑148 (MQ=255)
                                                     cTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGcgcg                                                                                           <  2:2316265/148‑1 (MQ=255)
                                                      tGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGa                                                                                          <  2:2533879/148‑1 (MQ=255)
                                                            ttACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCggg                                                                                    <  2:917234/148‑1 (MQ=255)
                                                            ttACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCggg                                                                                    <  1:3018956/148‑1 (MQ=255)
                                                            ttACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCggg                                                                                    <  1:160706/148‑1 (MQ=255)
                                                             tACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGa                                                                                   <  1:3428215/148‑1 (MQ=255)
                                                             tACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGa                                                                                   <  1:3428161/148‑1 (MQ=255)
                                                             tACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGa                                                                                   <  2:426441/148‑1 (MQ=255)
                                                                aTTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGAAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTg                                                                                <  1:3356392/148‑1 (MQ=255)
                                                                 ttCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGt                                                                               <  1:3360187/148‑1 (MQ=255)
                                                                 ttCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGt                                                                               <  1:1739952/148‑1 (MQ=255)
                                                                 ttCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGt                                                                               <  1:1740642/148‑1 (MQ=255)
                                                                 ttCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGt                                                                               >  1:3133600/1‑148 (MQ=255)
                                                                 ttCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTGACCGCGCGGCTGGCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGt                                                                               <  2:1819862/148‑1 (MQ=255)
                                                                       gCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAgg                                                                         >  2:1744800/1‑148 (MQ=255)
                                                                       gCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAgg                                                                         >  1:1374523/1‑148 (MQ=255)
                                                                          aGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAg                                                                      <  2:1439534/148‑1 (MQ=255)
                                                                          aGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAg                                                                      <  2:1437706/148‑1 (MQ=255)
                                                                            tctcAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGtc                                                                    <  1:1452403/148‑1 (MQ=255)
                                                                             ctcAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGtct                                                                   >  1:1674921/1‑148 (MQ=255)
                                                                              tcAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGtctt                                                                  <  2:92319/148‑1 (MQ=255)
                                                                               cAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGtcttc                                                                 <  2:375335/148‑1 (MQ=255)
                                                                               cAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGtcttc                                                                 >  1:522141/1‑148 (MQ=255)
                                                                               cAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGtcttc                                                                 <  2:375296/148‑1 (MQ=255)
                                                                                aaaGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGtcttct                                                                >  2:2137939/1‑148 (MQ=255)
                                                                                  aGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCttcttt                                                              <  2:955867/148‑1 (MQ=255)
                                                                                  aGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCttcttt                                                              >  1:271295/1‑148 (MQ=255)
                                                                                    cTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCttctttct                                                            <  2:1047865/148‑1 (MQ=255)
                                                                                    cTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCttctttct                                                            <  1:2929331/148‑1 (MQ=255)
                                                                                      ttCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTttcttc                                                          <  1:301656/148‑1 (MQ=255)
                                                                                      ttCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTttcttc                                                          >  1:289226/1‑148 (MQ=255)
                                                                                      ttCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTttcttc                                                          <  1:2052566/148‑1 (MQ=255)
                                                                                      ttCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTttcttc                                                          <  1:2052283/148‑1 (MQ=255)
                                                                                      ttCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTttcttc                                                          <  2:1413611/148‑1 (MQ=255)
                                                                                         gcgGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCcac                                                       <  1:594494/148‑1 (MQ=255)
                                                                                             tggtTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCg                                                   >  2:1427885/1‑148 (MQ=255)
                                                                                             tggtTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCg                                                   >  1:123128/1‑148 (MQ=255)
                                                                                              ggtTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGc                                                  <  1:2817870/148‑1 (MQ=255)
                                                                                                ttttCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCa                                                <  2:2774393/148‑1 (MQ=255)
                                                                                                 tttCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAg                                               <  1:2525181/148‑1 (MQ=255)
                                                                                                  ttCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGt                                              <  1:2467479/148‑1 (MQ=255)
                                                                                                         cgGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTAct                                       >  2:2046007/1‑148 (MQ=255)
                                                                                                          ggCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTActc                                      <  2:2592929/148‑1 (MQ=255)
                                                                                                           gCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTActct                                     <  1:1492228/148‑1 (MQ=255)
                                                                                                                  ccGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACa                              >  1:1830461/1‑148 (MQ=255)
                                                                                                                  ccGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACAACGCCAGTTCGTACTCTTGCGACa                              >  2:2649686/1‑148 (MQ=255)
                                                                                                                    gTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGt                            <  2:499352/148‑1 (MQ=255)
                                                                                                                     ttGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGtt                           >  1:2537686/1‑148 (MQ=255)
                                                                                                                     ttGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGtt                           >  2:983582/1‑148 (MQ=255)
                                                                                                                      tgtgCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGttt                          >  1:2765767/1‑148 (MQ=255)
                                                                                                                      tgtgCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGttt                          >  1:2843427/1‑148 (MQ=255)
                                                                                                                        tgCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGa                        <  2:1203726/148‑1 (MQ=255)
                                                                                                                            tAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATcgc                    >  2:3223482/1‑148 (MQ=255)
                                                                                                                              agcagcGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATcgccg                  >  1:2659435/1‑148 (MQ=255)
                                                                                                                                cagcGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCgccgcc                >  2:310015/1‑148 (MQ=255)
                                                                                                                                cagcGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCgccgcc                >  2:3510849/1‑148 (MQ=255)
                                                                                                                                 agcGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGccgccg               >  2:2223136/1‑148 (MQ=255)
                                                                                                                                   cgcAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCCGTc             >  1:517148/1‑148 (MQ=255)
                                                                                                                                    gcAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCCGTCg            <  1:93668/148‑1 (MQ=255)
                                                                                                                                     cAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCCGTCgg           >  1:811095/1‑148 (MQ=255)
                                                                                                                                     cAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCCGTCgg           >  2:1206860/1‑148 (MQ=255)
                                                                                                                                      aaTGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCCGTCggg          <  1:3304364/148‑1 (MQ=255)
                                                                                                                                       aTGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCCGTCGGGc         <  1:1865361/148‑1 (MQ=255)
                                                                                                                                           cGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCCGTCGGGCAGAc     <  1:2775493/148‑1 (MQ=255)
                                                                                                                                             tagtagATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCCGTCGGGCAGActt   <  2:1632835/148‑1 (MQ=255)
                                                                                                                                              aGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCCGTCGGGCAGActtc  <  1:1309971/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GCCTCTTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCCGTCGGGCAGACTTC  >  CP009273/2837576‑2837865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GATGGCCTCTTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCCGTCGGGCAGACTTCTTCGC  >  CP009273/2837572‑2837870
                                                                                                                                                     |                                                                                                                                                     
GATGGCCTCTTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTA                                                                                                                                                       <  SRR19973067.468233/150‑1 (MQ=60)
  TGGCCTCTTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGA                                                                                                                                                     <  SRR19973067.1985524/150‑1 (MQ=60)
   GGCCTCTTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTACCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGAT                                                                                                                                                    >  SRR19973067.2609808/1‑150 (MQ=60)
    GCCTCTTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATC                                                                                                                                                   <  SRR19973067.524291/150‑1 (MQ=60)
    GCCTCTTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATC                                                                                                                                                   <  SRR19973067.524352/150‑1 (MQ=60)
         TTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTT                                                                                                                                              <  SRR19973067.1773592/150‑1 (MQ=60)
         TTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTT                                                                                                                                              <  SRR19973067.326498/150‑1 (MQ=60)
           TCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCT                                                                                                                                            <  SRR19973067.1138516/150‑1 (MQ=60)
           TCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCT                                                                                                                                            <  SRR19973067.787181/150‑1 (MQ=60)
           TCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCT                                                                                                                                            <  SRR19973067.2499630/150‑1 (MQ=60)
           TCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCT                                                                                                                                            <  SRR19973067.1138561/150‑1 (MQ=60)
              TATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGA                                                                                                                                         >  SRR19973067.1644068/1‑150 (MQ=60)
                TGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACG                                                                                                                                       <  SRR19973067.1963328/150‑1 (MQ=60)
                  GCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGC                                                                                                                                     <  SRR19973067.2554663/150‑1 (MQ=60)
                    GAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGA                                                                                                                                   >  SRR19973067.1527202/1‑150 (MQ=60)
                    GAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGA                                                                                                                                   >  SRR19973067.2266127/1‑150 (MQ=60)
                         AGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGA                                                                                                                              >  SRR19973067.2208147/1‑150 (MQ=60)
                         AGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGA                                                                                                                              >  SRR19973067.2207642/1‑150 (MQ=60)
                         AGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGA                                                                                                                              >  SRR19973067.2176483/1‑150 (MQ=60)
                              AATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATT                                                                                                                         >  SRR19973067.3454461/1‑150 (MQ=60)
                                TACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGC                                                                                                                       >  SRR19973067.1864648/1‑150 (MQ=60)
                                     TCGGACGGCACCAGGCATTTCTGGCGATTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTTCTTAAGCATCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACG                                                                                                                  <  SRR19973067.1709718/150‑1 (MQ=60)
                                         ACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGC                                                                                                              <  SRR19973067.2723015/150‑1 (MQ=60)
                                             CACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTT                                                                                                          <  SRR19973067.509025/150‑1 (MQ=60)
                                              ACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTG                                                                                                         >  SRR19973067.1965811/1‑150 (MQ=60)
                                              ACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGGAGGAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTG                                                                                                         >  SRR19973067.347174/1‑150 (MQ=60)
                                                  GGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACA                                                                                                     >  SRR19973067.1791133/1‑150 (MQ=60)
                                                  GGCATTTCTGGCGCTTACATTCCGGGCAAGTATCAAAGCATTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCATAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGAAGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACA                                                                                                     >  SRR19973067.3252897/1‑150 (MQ=60)
                                                     ATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCG                                                                                                  <  SRR19973067.1054801/150‑1 (MQ=60)
                                                     ATTTCTGGCGCTTACATTCCGGGCAAGTCTAAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCG                                                                                                  <  SRR19973067.1933533/150‑1 (MQ=60)
                                                       TTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCG                                                                                                <  SRR19973067.1538608/150‑1 (MQ=60)
                                                             CGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGG                                                                                          >  SRR19973067.2947185/1‑150 (MQ=60)
                                                              GCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGG                                                                                         <  SRR19973067.2877013/150‑1 (MQ=60)
                                                                TTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGAC                                                                                       <  SRR19973067.3038175/150‑1 (MQ=60)
                                                                TTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGAC                                                                                       <  SRR19973067.3038737/150‑1 (MQ=60)
                                                                TTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGAC                                                                                       <  SRR19973067.162035/150‑1 (MQ=60)
                                                                 TACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACT                                                                                      <  SRR19973067.3449976/150‑1 (MQ=60)
                                                                 TACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACT                                                                                      <  SRR19973067.3450030/150‑1 (MQ=60)
                                                                 TACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACT                                                                                      <  SRR19973067.3449978/150‑1 (MQ=60)
                                                                   CATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGT                                                                                    >  SRR19973067.3153507/1‑150 (MQ=60)
                                                                    ATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTT                                                                                   >  SRR19973067.2169677/1‑150 (MQ=60)
                                                                    ATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGAAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTT                                                                                   <  SRR19973067.3377695/150‑1 (MQ=60)
                                                                     TTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTG                                                                                  <  SRR19973067.1751844/150‑1 (MQ=60)
                                                                     TTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTG                                                                                  <  SRR19973067.1752538/150‑1 (MQ=60)
                                                                     TTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTG                                                                                  <  SRR19973067.3381521/150‑1 (MQ=60)
                                                                         GGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGG                                                                              >  SRR19973067.943833/1‑150 (MQ=60)
                                                                         GGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGG                                                                              >  SRR19973067.1383905/1‑150 (MQ=60)
                                                                         GGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGG                                                                              >  SRR19973067.3289764/1‑150 (MQ=60)
                                                                               GTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCT                                                                        >  SRR19973067.1686469/1‑150 (MQ=60)
                                                                                TCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTT                                                                       <  SRR19973067.1616678/150‑1 (MQ=60)
                                                                                TCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTT                                                                       <  SRR19973067.1462077/150‑1 (MQ=60)
                                                                                TCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTT                                                                       <  SRR19973067.1462130/150‑1 (MQ=60)
                                                                                 CTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTC                                                                      >  SRR19973067.525665/1‑150 (MQ=60)
                                                                                    AAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTT                                                                   >  SRR19973067.273130/1‑150 (MQ=60)
                                                                                    AAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTT                                                                   >  SRR19973067.273110/1‑150 (MQ=60)
                                                                                     AAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTC                                                                  <  SRR19973067.2798986/150‑1 (MQ=60)
                                                                                        CTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTC                                                               >  SRR19973067.291099/1‑150 (MQ=60)
                                                                                        CTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTC                                                               <  SRR19973067.2947937/150‑1 (MQ=60)
                                                                                        CTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTC                                                               <  SRR19973067.2948009/150‑1 (MQ=60)
                                                                                          TTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCA                                                             <  SRR19973067.2066262/150‑1 (MQ=60)
                                                                                          TTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCA                                                             <  SRR19973067.2065979/150‑1 (MQ=60)
                                                                                          TTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCA                                                             <  SRR19973067.2066011/150‑1 (MQ=60)
                                                                                          TTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCA                                                             <  SRR19973067.303583/150‑1 (MQ=60)
                                                                                             GCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACAC                                                          <  SRR19973067.598270/150‑1 (MQ=60)
                                                                                               GGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCG                                                        >  SRR19973067.124275/1‑150 (MQ=60)
                                                                                                  GGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCA                                                     <  SRR19973067.2836014/150‑1 (MQ=60)
                                                                                                     TTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTT                                                  <  SRR19973067.2541729/150‑1 (MQ=60)
                                                                                                      TTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTC                                                 <  SRR19973067.2483703/150‑1 (MQ=60)
                                                                                                               GCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTG                                        <  SRR19973067.1502092/150‑1 (MQ=60)
                                                                                                                    CGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACA                                   >  SRR19973067.1842764/1‑150 (MQ=60)
                                                                                                                    CGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACA                                   >  SRR19973067.1842904/1‑150 (MQ=60)
                                                                                                                       CGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTT                                >  SRR19973067.2553636/1‑150 (MQ=60)
                                                                                                                       CGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTT                                >  SRR19973067.2554301/1‑150 (MQ=60)
                                                                                                                       CGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTT                                >  SRR19973067.128775/1‑150 (MQ=60)
                                                                                                                        GTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTT                               >  SRR19973067.2861680/1‑150 (MQ=60)
                                                                                                                        GTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTT                               >  SRR19973067.2783639/1‑150 (MQ=60)
                                                                                                                                TAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCG                       <  SRR19973067.2353901/150‑1 (MQ=60)
                                                                                                                                TAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCG                       >  SRR19973067.2676548/1‑150 (MQ=60)
                                                                                                                                     AGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCCGTC                  >  SRR19973067.580871/1‑150 (MQ=60)
                                                                                                                                     AGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCCGTC                  >  SRR19973067.520651/1‑150 (MQ=60)
                                                                                                                                       CGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCCGTCGG                >  SRR19973067.816662/1‑150 (MQ=60)
                                                                                                                                       CGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCCGTCGG                >  SRR19973067.816668/1‑150 (MQ=60)
                                                                                                                                        GCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCCGTCGGG               <  SRR19973067.94651/150‑1 (MQ=60)
                                                                                                                                        GCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCCGTCGGG               <  SRR19973067.1905261/150‑1 (MQ=60)
                                                                                                                                          AATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCCGTCGGGCA             <  SRR19973067.3325426/150‑1 (MQ=60)
                                                                                                                                          AATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCCGTCGGGCA             <  SRR19973067.3325206/150‑1 (MQ=60)
                                                                                                                                           ATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCCGTCGGGCAG            <  SRR19973067.1878090/150‑1 (MQ=60)
                                                                                                                                               CGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCCGTCGGGCAGACTT        <  SRR19973067.2793421/150‑1 (MQ=60)
                                                                                                                                               CGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCCGTCGGGCAGACTT        <  SRR19973067.2792517/150‑1 (MQ=60)
                                                                                                                                                 TAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCCGTCGGGCAGACTTCT      <  SRR19973067.3380192/150‑1 (MQ=60)
                                                                                                                                                  AGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCCGTCGGGCAGACTTCTT     <  SRR19973067.1318913/150‑1 (MQ=60)
                                                                                                                                                  AGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCCGTCGGGCAGACTTCTT     <  SRR19973067.1318912/150‑1 (MQ=60)
                                                                                                                                                   GTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCCGTCGGGCAGACTTCTTC    <  SRR19973067.2638852/150‑1 (MQ=60)
                                                                                                                                                   GTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCCGTCGGGCAGACTTCTTC    <  SRR19973067.847965/150‑1 (MQ=60)
                                                                                                                                                    TAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCCGTCGGGCAGACTTCTTCG   >  SRR19973067.338249/1‑150 (MQ=60)
                                                                                                                                                    TAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCCGTCGGGCAGACTTCTTCG   >  SRR19973067.338067/1‑150 (MQ=60)
                                                                                                                                                     AGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCCGTCGGGCAGACTTCTTCGC  >  SRR19973067.1614762/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
GATGGCCTCTTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCCGTCGGGCAGACTTCTTCGC  >  CP009273/2837572‑2837870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑