Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,837,721 C→A 100% D131Y (GAC→TAC)  hycF ← formate hydrogenlyase complex iron‑sulfur protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,837,7210CA96.0% 553.7 / 12.7 185D131Y (GAC→TAC) hycFformate hydrogenlyase complex iron‑sulfur protein
Reads supporting (aligned to +/- strand):  ref base C (6/2);  new base A (82/95);  total (88/97)
Fisher's exact test for biased strand distribution p-value = 1.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 186 total aligned reads displayed.

GCCTCTTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCCGTCGGGCAGACTTC  >  CP009273/2837576‑2837865
                                                                                                                                                 |                                                                                                                                                
   tctTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGAtct                                                                                                                                             >  2:1063622/1‑148 (MQ=255)
    ctTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGAtctc                                                                                                                                            <  1:2696736/148‑1 (MQ=255)
       tCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAATGCTTTCGCGGTGGTTTTCCGCGCGTCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTATATCTCttt                                                                                                                                         <  1:3420430/148‑1 (MQ=255)
       tCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCttt                                                                                                                                         >  2:741598/1‑148 (MQ=255)
       tCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCttt                                                                                                                                         >  1:2250555/1‑148 (MQ=255)
          tatGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTg                                                                                                                                      >  2:2456449/1‑148 (MQ=255)
                gAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGc                                                                                                                                <  2:1395325/148‑1 (MQ=255)
                gAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGc                                                                                                                                <  2:3169492/148‑1 (MQ=255)
                  gTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGa                                                                                                                              >  1:3074045/1‑148 (MQ=255)
                    cAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGaaa                                                                                                                            <  1:233032/148‑1 (MQ=255)
                     aGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAg                                                                                                                           <  1:313487/148‑1 (MQ=255)
                      gTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAgg                                                                                                                          <  1:2389949/148‑1 (MQ=255)
                       ttCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGa                                                                                                                         >  2:2970578/1‑148 (MQ=255)
                         cAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACg                                                                                                                       <  1:1423841/148‑1 (MQ=255)
                              cGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGc                                                                                                                  <  2:2586611/148‑1 (MQ=255)
                              cGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGc                                                                                                                  <  2:1804755/148‑1 (MQ=255)
                               ggTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCa                                                                                                                 <  1:2828759/148‑1 (MQ=255)
                                 tCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGa                                                                                                               >  1:1351647/1‑148 (MQ=255)
                                 tCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGa                                                                                                               <  2:1983486/148‑1 (MQ=255)
                                     aCGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAATCAGCGCAATGGCGTAGTCGATATCTTTATTGAAGGCGAAAGGACGATTGCAGAcgcg                                                                                                           >  1:14158/1‑148 (MQ=255)
                                     aCGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCGGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGATATCTTTATGGTCGGCGAAAGGTCGATTGCAGAcgcg                                                                                                           >  1:14467/1‑148 (MQ=255)
                                      cGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCgg                                                                                                          <  2:3063892/148‑1 (MQ=255)
                                         cACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAg                                                                                                       <  1:3515923/148‑1 (MQ=255)
                                         cACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAAATGCGTAGTAGATATCTTTCTGGACGGCGAAAGGACGATTTCAGACGCGGCAg                                                                                                       >  1:2688158/1‑148 (MQ=255)
                                                   ttCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAgcg                                                                                             >  2:3335168/1‑148 (MQ=255)
                                                        gcgcTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAg                                                                                        <  2:2308873/148‑1 (MQ=255)
                                                          gcTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCg                                                                                      <  1:1329715/148‑1 (MQ=255)
                                                           cTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCgg                                                                                     >  1:99120/1‑148 (MQ=255)
                                                              aCATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGAc                                                                                  >  2:54696/1‑148 (MQ=255)
                                                                 ttCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGt                                                                               <  1:1824119/148‑1 (MQ=255)
                                                                  tCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGtt                                                                              >  1:178940/1‑148 (MQ=255)
                                                                     gggCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCa                                                                           <  2:3098661/148‑1 (MQ=255)
                                                                     gggCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCa                                                                           <  1:2441072/148‑1 (MQ=255)
                                                                      ggCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAg                                                                          >  2:1558735/1‑148 (MQ=255)
                                                                          aGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACCGCGCGACGCGGGACTGTTGCAGGAAg                                                                      >  2:2537283/1‑148 (MQ=255)
                                                                          aGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAg                                                                      >  2:2537300/1‑148 (MQ=255)
                                                                           gTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGt                                                                     <  2:1154810/148‑1 (MQ=255)
                                                                            tctcAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGtc                                                                    >  2:2531048/1‑148 (MQ=255)
                                                                            tctcAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGtc                                                                    >  2:3434576/1‑148 (MQ=255)
                                                                             ctcAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGtct                                                                   >  2:754411/1‑148 (MQ=255)
                                                                               cAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGc                                                                                       >  2:1468624/1‑126 (MQ=255)
                                                                               cAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGc                                                                                       <  1:1468624/126‑1 (MQ=255)
                                                                               cAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGtcttc                                                                 <  1:1947945/148‑1 (MQ=255)
                                                                               cAAAGCTGTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGCCGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGtcttc                                                                 <  2:2633024/148‑1 (MQ=255)
                                                                                 aaGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTcttctt                                                               <  2:109086/148‑1 (MQ=255)
                                                                                 aaGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTcttctt                                                               <  2:484053/148‑1 (MQ=255)
                                                                                  aGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCttcttt                                                              >  2:275756/1‑148 (MQ=255)
                                                                                  aGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCttcttt                                                              >  1:3324440/1‑148 (MQ=255)
                                                                                   gCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCttctttc                                                             <  2:448218/148‑1 (MQ=255)
                                                                                   gCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCttctttc                                                             <  1:1755546/148‑1 (MQ=255)
                                                                                    cTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCttctttct                                                            >  2:2364118/1‑148 (MQ=255)
                                                                                      ttCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTttcttc                                                          <  2:2210718/148‑1 (MQ=255)
                                                                                      ttCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTttcttc                                                          <  1:3245404/148‑1 (MQ=255)
                                                                                      ttCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTttcttc                                                          >  2:1439038/1‑148 (MQ=255)
                                                                                      ttCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTttcttc                                                          >  1:152382/1‑148 (MQ=255)
                                                                                      ttCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAAAGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTttcttc                                                          >  1:318435/1‑148 (MQ=255)
                                                                                       tCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTcc                                                         <  1:54696/148‑1 (MQ=255)
                                                                                          cgGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCcaca                                                      <  1:299132/148‑1 (MQ=255)
                                                                                             tggtTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCg                                                   >  2:113673/1‑148 (MQ=255)
                                                                                             tggtTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCg                                                   >  2:3007804/1‑148 (MQ=255)
                                                                                             gggtTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATGTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCGTCGTTCTTCCACACCg                                                   >  1:620991/2‑148 (MQ=255)
                                                                                              ggtTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGc                                                  >  1:2896626/1‑148 (MQ=255)
                                                                                              ggtTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGc                                                  <  2:1866877/148‑1 (MQ=255)
                                                                                              ggtTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGc                                                  <  2:63215/148‑1 (MQ=255)
                                                                                              ggtTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGc                                                  >  1:1117770/1‑148 (MQ=255)
                                                                                              ggtTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGc                                                  >  1:358948/1‑148 (MQ=255)
                                                                                              ggtTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTCTCTTCCACACCGc                                                  <  2:1117295/148‑1 (MQ=255)
                                                                                               gtTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGcc                                                 <  1:265866/148‑1 (MQ=255)
                                                                                                 tttCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAg                                               >  2:3472380/1‑148 (MQ=255)
                                                                                                       cgcgGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTa                                         <  2:2243046/148‑1 (MQ=255)
                                                                                                       cgcgGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTa                                         <  2:2807881/148‑1 (MQ=255)
                                                                                                         cgGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTAct                                       <  2:317262/148‑1 (MQ=255)
                                                                                                         cgGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTAct                                       <  2:317232/148‑1 (MQ=255)
                                                                                                         cgGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTAct                                       <  1:413185/148‑1 (MQ=255)
                                                                                                           gCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTActct                                     <  2:361254/148‑1 (MQ=255)
                                                                                                           gCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTActct                                     <  2:651471/148‑1 (MQ=255)
                                                                                                           gCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTActct                                     <  2:3153267/148‑1 (MQ=255)
                                                                                                             tGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTg                                   >  1:1036355/1‑148 (MQ=255)
                                                                                                                  ccGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACa                              <  2:2429377/148‑1 (MQ=255)
                                                                                                                     ttGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGtt                           >  1:2875035/1‑148 (MQ=255)
                                                                                                                      tgtgCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGttt                          >  1:2336701/1‑148 (MQ=255)
                                                                                                                      tgtgCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGttt                          >  1:2113986/1‑148 (MQ=255)
                                                                                                                        tgCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGa                        <  2:2988038/148‑1 (MQ=255)
                                                                                                                        tgCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGa                        >  1:3511181/1‑148 (MQ=255)
                                                                                                                         gCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGAt                       <  2:1309141/148‑1 (MQ=255)
                                                                                                                           ttAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATcg                     >  2:1278590/1‑148 (MQ=255)
                                                                                                                            tAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATcgc                    <  1:2196162/148‑1 (MQ=255)
                                                                                                                            tAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATcgc                    <  1:3443478/148‑1 (MQ=255)
                                                                                                                              agcagcGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATcgccg                  <  1:305221/148‑1 (MQ=255)
                                                                                                                              agcagcGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATcgccg                  <  2:3222504/148‑1 (MQ=255)
                                                                                                                                cagcGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCgccgcc                <  1:1891165/148‑1 (MQ=255)
                                                                                                                                 agcGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGccgccg               <  1:3182900/148‑1 (MQ=255)
                                                                                                                                 agcGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGccgccg               <  1:3183445/148‑1 (MQ=255)
                                                                                                                                  gcgcAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCCGt              >  2:715917/1‑148 (MQ=255)
                                                                                                                                    gcAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCCGTCg            <  1:1285492/148‑1 (MQ=255)
                                                                                                                                     cAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCCGTCgg           >  1:2939152/1‑148 (MQ=255)
                                                                                                                                      aaTGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCCGTCggg          <  2:178940/148‑1 (MQ=255)
                                                                                                                                         ggCGTAGTCGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCCGTCGGGCAg       >  1:1034680/1‑148 (MQ=255)
                                                                                                                                         ggCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCCGTCGGGCAg       >  1:1667537/1‑148 (MQ=255)
                                                                                                                                             tagtagATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCCGTCGGGCAGActt   <  2:269222/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GCCTCTTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCCGTCGGGCAGACTTC  >  CP009273/2837576‑2837865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GATGGCCTCTTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCCGTCGGGCAGACTTCTTC  >  CP009273/2837572‑2837868
                                                                                                                                                     |                                                                                                                                                   
GATGGCCTCTTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTA                                                                                                                                                     <  SRR19973066.2767307/150‑1 (MQ=60)
  TGGCCTCTTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGA                                                                                                                                                   <  SRR19973066.1173087/150‑1 (MQ=60)
    GCCTCTTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATC                                                                                                                                                 <  SRR19973066.3444744/150‑1 (MQ=60)
     CCTCTTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCT                                                                                                                                                >  SRR19973066.115538/1‑150 (MQ=60)
        CTTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTT                                                                                                                                             <  SRR19973066.2714762/150‑1 (MQ=60)
         TTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTT                                                                                                                                            >  SRR19973066.2266072/1‑150 (MQ=60)
          TTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTC                                                                                                                                           <  SRR19973066.2953746/150‑1 (MQ=60)
           TCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAATGCTTTCGCGGTGGTTTTCCGCGCGTCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTATATCTCTTTCT                                                                                                                                          <  SRR19973066.3443306/150‑1 (MQ=60)
           TCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCT                                                                                                                                          <  SRR19973066.1666041/150‑1 (MQ=60)
           TCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCT                                                                                                                                          <  SRR19973066.2284540/150‑1 (MQ=60)
             ATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGG                                                                                                                                        <  SRR19973066.3064382/150‑1 (MQ=60)
              TATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGA                                                                                                                                       <  SRR19973066.171260/150‑1 (MQ=60)
                    GAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGA                                                                                                                                 >  SRR19973066.3094589/1‑150 (MQ=60)
                        CAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGG                                                                                                                             <  SRR19973066.234877/150‑1 (MQ=60)
                         AGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGA                                                                                                                            >  SRR19973066.1318639/1‑150 (MQ=60)
                         AGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGA                                                                                                                            <  SRR19973066.315803/150‑1 (MQ=60)
                          GTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGAC                                                                                                                           <  SRR19973066.2406036/150‑1 (MQ=60)
                           TTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACG                                                                                                                          <  SRR19973066.409961/150‑1 (MQ=60)
                             CAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGAT                                                                                                                        <  SRR19973066.1434021/150‑1 (MQ=60)
                                   GGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGA                                                                                                                  <  SRR19973066.2847764/150‑1 (MQ=60)
                                   GGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGA                                                                                                                  >  SRR19973066.1361436/1‑150 (MQ=60)
                                   GGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGA                                                                                                                  <  SRR19973066.2848645/150‑1 (MQ=60)
                                       GGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAATCAGCGCAATGGCGTAGTCGATATCTTTATTGAAGGCGAAAGGACGATTGCAGACGCG                                                                                                              >  SRR19973066.14381/1‑150 (MQ=60)
                                       GGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCG                                                                                                              >  SRR19973066.3380545/1‑150 (MQ=60)
                                       GGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCGGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGATATCTTTATGGTCGGCGAAAGGTCGATTGCAGACGCG                                                                                                              >  SRR19973066.14692/1‑150 (MQ=60)
                                           GGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAAATGCGTAGTAGATATCTTTCTGGACGGCGAAAGGACGATTTCAGACGCGGCAG                                                                                                          >  SRR19973066.2706096/1‑150 (MQ=60)
                                             CACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTT                                                                                                        <  SRR19973066.3539337/150‑1 (MQ=60)
                                               CCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGC                                                                                                      >  SRR19973066.271220/1‑150 (MQ=60)
                                                CAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAAAGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCA                                                                                                     >  SRR19973066.2445901/1‑150 (MQ=60)
                                                           GGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGC                                                                                          >  SRR19973066.360659/1‑150 (MQ=60)
                                                            GCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCG                                                                                         <  SRR19973066.1826704/150‑1 (MQ=60)
                                                             gacgtgtgctcttccgatcTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGG                                                                                        <  SRR19973066.1478984/131‑1 (MQ=60)
                                                             gacgtgtgctcttccgatcTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGG                                                                                        <  SRR19973066.1478988/131‑1 (MQ=60)
                                                             CGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGG                                                                                        >  SRR19973066.100181/1‑150 (MQ=60)
                                                              GCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGG                                                                                       <  SRR19973066.1339354/150‑1 (MQ=60)
                                                               CTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGA                                                                                      <  SRR19973066.2350239/150‑1 (MQ=60)
                                                                 TACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACT                                                                                    <  SRR19973066.2732266/150‑1 (MQ=60)
                                                                    ATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTT                                                                                 >  SRR19973066.180511/1‑150 (MQ=60)
                                                                     TTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTG                                                                                <  SRR19973066.1837129/150‑1 (MQ=60)
                                                                         GGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGG                                                                            <  SRR19973066.2457699/150‑1 (MQ=60)
                                                                              AGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTC                                                                       <  SRR19973066.2701266/150‑1 (MQ=60)
                                                                               GTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCT                                                                      <  SRR19973066.3303114/150‑1 (MQ=60)
                                                                                   CAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTT                                                                  <  SRR19973066.2118474/150‑1 (MQ=60)
                                                                                   CAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTT                                                                  <  SRR19973066.1961744/150‑1 (MQ=60)
                                                                                   CAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTT                                                                  <  SRR19973066.1961083/150‑1 (MQ=60)
                                                                                    AAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTT                                                                 >  SRR19973066.3346526/1‑150 (MQ=60)
                                                                                      AGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCT                                                               >  SRR19973066.599937/1‑150 (MQ=60)
                                                                                       GCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTT                                                              <  SRR19973066.1768164/150‑1 (MQ=60)
                                                                                        CTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTC                                                             >  SRR19973066.153803/1‑150 (MQ=60)
                                                                                        CTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTC                                                             >  SRR19973066.320785/1‑150 (MQ=60)
                                                                                        CTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTC                                                             >  SRR19973066.2130541/1‑150 (MQ=60)
                                                                                        CTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAAAGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTC                                                             >  SRR19973066.320822/1‑150 (MQ=60)
                                                                                         TTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCC                                                            <  SRR19973066.3134686/150‑1 (MQ=60)
                                                                                          TTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCA                                                           <  SRR19973066.3266801/150‑1 (MQ=60)
                                                                                          TTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCA                                                           <  SRR19973066.3251996/150‑1 (MQ=60)
                                                                                           TCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCAC                                                          <  SRR19973066.55387/150‑1 (MQ=60)
                                                                                              CGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACC                                                       <  SRR19973066.2494317/150‑1 (MQ=60)
                                                                                              CGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACC                                                       <  SRR19973066.301243/150‑1 (MQ=60)
                                                                                               GGGGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATGTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCGTCGTTCTTCCACACCG                                                      >  SRR19973066.625406/1‑150 (MQ=60)
                                                                                                GTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGC                                                     >  SRR19973066.2915987/1‑150 (MQ=60)
                                                                                                GTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGC                                                     >  SRR19973066.1125830/1‑150 (MQ=60)
                                                                                                GTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGC                                                     >  SRR19973066.361748/1‑150 (MQ=60)
                                                                                                   GTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAG                                                  <  SRR19973066.267855/150‑1 (MQ=60)
                                                                                                    TTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGT                                                 <  SRR19973066.1428002/150‑1 (MQ=60)
                                                                                                             CGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCT                                        <  SRR19973066.416415/150‑1 (MQ=60)
                                                                                                               GCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTG                                      >  SRR19973066.1043985/1‑150 (MQ=60)
                                                                                                               GCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTG                                      >  SRR19973066.1043980/1‑150 (MQ=60)
                                                                                                                CTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGC                                     >  SRR19973066.3176950/1‑150 (MQ=60)
                                                                                                                     GCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAG                                >  SRR19973066.421138/1‑150 (MQ=60)
                                                                                                                     GCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAG                                >  SRR19973066.2229000/1‑150 (MQ=60)
                                                                                                                       CGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTT                              >  SRR19973066.2894303/1‑150 (MQ=60)
                                                                                                                       CGTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTT                              <  SRR19973066.2982460/150‑1 (MQ=60)
                                                                                                                        GTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTT                             >  SRR19973066.2352626/1‑150 (MQ=60)
                                                                                                                        GTTGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTT                             >  SRR19973066.2128601/1‑150 (MQ=60)
                                                                                                                          TGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGA                           >  SRR19973066.3534569/1‑150 (MQ=60)
                                                                                                                          TGTGCTTAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGA                           >  SRR19973066.1794382/1‑150 (MQ=60)
                                                                                                                               TTAAGCAGCGCAATGGCGTAGTCGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCC                      <  SRR19973066.3511104/150‑1 (MQ=60)
                                                                                                                                TAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCG                     <  SRR19973066.3466517/150‑1 (MQ=60)
                                                                                                                                TAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCG                     >  SRR19973066.705457/1‑150 (MQ=60)
                                                                                                                                TAAGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCG                     <  SRR19973066.2211393/150‑1 (MQ=60)
                                                                                                                                  AGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCC                   <  SRR19973066.307465/150‑1 (MQ=60)
                                                                                                                                  AGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCC                   <  SRR19973066.307405/150‑1 (MQ=60)
                                                                                                                                  AGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCC                   >  SRR19973066.1360525/1‑150 (MQ=60)
                                                                                                                                  AGCAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCC                   >  SRR19973066.1361586/1‑150 (MQ=60)
                                                                                                                                    CAGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCCGT                 <  SRR19973066.1904647/150‑1 (MQ=60)
                                                                                                                                     AGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCCGTC                <  SRR19973066.3204073/150‑1 (MQ=60)
                                                                                                                                     AGCGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCCGTC                <  SRR19973066.3204621/150‑1 (MQ=60)
                                                                                                                                       CGCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCCGTCGG              >  SRR19973066.2958693/1‑150 (MQ=60)
                                                                                                                                        GCAATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCCGTCGGG             <  SRR19973066.1294782/150‑1 (MQ=60)
                                                                                                                                           ATGGCGTAGTCGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCCGTCGGGCAG          >  SRR19973066.1042295/1‑150 (MQ=60)
                                                                                                                                           ATGGCGTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCCGTCGGGCAG          >  SRR19973066.1679689/1‑150 (MQ=60)
                                                                                                                                                GTAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCCGTCGGGCAGACTTC     >  SRR19973066.1460393/1‑150 (MQ=60)
                                                                                                                                                 TAGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCCGTCGGGCAGACTTCT    >  SRR19973066.3549557/1‑150 (MQ=60)
                                                                                                                                                  AGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCCGTCGGGCAGACTTCTT   >  SRR19973066.1856419/1‑150 (MQ=60)
                                                                                                                                                  AGTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGGCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCCGTCGGGCAGACTTCTT   >  SRR19973066.38961/1‑150 (MQ=60)
                                                                                                                                                   GTAGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCCGTCGGGCAGACTTCTNC  <  SRR19973066.1480293/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                   
GATGGCCTCTTTCATATGGCGAGTCAGTTCAATACGGTCGGACGGCACCAGGCATTTCTGGCGCTTACATTCCGGGCAAGTCTCAAAGCTTTCGCGGTGGTTTTCCGCGCGGCTGTCGCCGTTGTGCTTAAGCAGCGCAATGGCGTAGTCGATCTCTTTCTGGACGGCGAAAGGACGATTGCAGACGCGGCAGTTGCACAGCGCGAAGCGGGACTGTTGCAGGAAGTCTTCTTTCTTCCACACCGCCAGTTCGTACTCTTGCGACAGTTTGATCGCCGCCGTCGGGCAGACTTCTTC  >  CP009273/2837572‑2837868

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑