Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,984,747 G→T 92.7% D41Y (GAT→TAT)  ygeG → SycD‑like chaperone family TPR‑repeat‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,984,7470GT92.7% 728.6 / 46.2 260D41Y (GAT→TAT) ygeGSycD‑like chaperone family TPR‑repeat‑containing protein
Reads supporting (aligned to +/- strand):  ref base G (10/9);  new base T (113/128);  total (123/137)
Fisher's exact test for biased strand distribution p-value = 6.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 262 total aligned reads displayed.

CACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTGATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAGTATGTCAGCCCAAAAAGAAA  >  CP009273/2984601‑2984893
                                                                                                                                                  |                                                                                                                                                  
cACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTGa                                                                                                                                                   >  1:941580/1‑148 (MQ=255)
cACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTGa                                                                                                                                                   >  1:1411073/1‑148 (MQ=255)
 aCGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTAt                                                                                                                                                  >  1:2803626/1‑148 (MQ=255)
  cGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTtata                                                                                                                                                 >  1:3147900/1‑148 (MQ=255)
    agaCACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGa                                                                                                                                               <  1:323212/148‑1 (MQ=255)
            cacaGAGGAATATGATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATatatgc                                                                                                                                       >  1:1753540/1‑148 (MQ=255)
              caGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATatatgcat                                                                                                                                     <  2:2457804/148‑1 (MQ=255)
              caGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATatatgcat                                                                                                                                     <  1:662981/148‑1 (MQ=255)
              caGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATatatgcat                                                                                                                                     <  1:1980593/148‑1 (MQ=255)
               agagGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATatatgcata                                                                                                                                    >  2:2607372/1‑148 (MQ=255)
                   gAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATAtatgcatatgca                                                                                                                                >  2:2642496/1‑148 (MQ=255)
                   gAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATAtatgcatatgca                                                                                                                                <  2:2852903/148‑1 (MQ=255)
                    aaTATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATatgcatatgcat                                                                                                                               <  1:2631480/148‑1 (MQ=255)
                    aaTATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATatgcatatgcat                                                                                                                               <  2:3483329/148‑1 (MQ=255)
                     atatTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCAtt                                                                                                                              <  2:289457/148‑1 (MQ=255)
                      tattatGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTc                                                                                                                             >  1:2167744/1‑148 (MQ=255)
                        ttatGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGa                                                                                                                           <  2:3218402/148‑1 (MQ=255)
                         tatGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGAc                                                                                                                          >  1:1545641/1‑148 (MQ=255)
                          atGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACt                                                                                                                         >  2:3383991/1‑148 (MQ=255)
                             aGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTAcc                                                                                                                      >  2:3021192/1‑148 (MQ=255)
                             aGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTAcc                                                                                                                      >  2:1249766/1‑148 (MQ=255)
                              gCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTGATAGAATATATGCATATGCATTCGACTACCa                                                                                                                     <  1:215514/148‑1 (MQ=255)
                                 caGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTGATAGAATATATGCATATGCATTCGACTACCATGa                                                                                                                  >  2:2955540/1‑148 (MQ=255)
                                  aGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGaa                                                                                                                 >  1:566174/1‑148 (MQ=255)
                                    aaaCAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGaaaa                                                                                                               <  1:1418632/148‑1 (MQ=255)
                                      aCAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAgg                                                                                                             >  2:2317014/1‑148 (MQ=255)
                                       cAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAggg                                                                                                            >  1:1461918/1‑148 (MQ=255)
                                        aaTTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGt                                                                                                           <  1:3350613/148‑1 (MQ=255)
                                         aTTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTa                                                                                                          >  2:936064/1‑148 (MQ=255)
                                         aTTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTa                                                                                                          <  2:2006380/148‑1 (MQ=255)
                                          ttGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTaa                                                                                                         >  2:765912/1‑148 (MQ=255)
                                           tGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAat                                                                                                        <  2:2095923/148‑1 (MQ=255)
                                            gAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAata                                                                                                       <  2:485724/148‑1 (MQ=255)
                                              aaTATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAAtata                                                                                                     >  1:1743410/1‑148 (MQ=255)
                                               atatTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGACTATATGCATATGCATTCGACTACCATGAAAAGGGTAATATaa                                                                                                    >  1:632144/1‑148 (MQ=255)
                                                 atTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACg                                                                                                  >  2:3232853/1‑148 (MQ=255)
                                                  ttCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACgg                                                                                                 <  2:756435/148‑1 (MQ=255)
                                                   tCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGa                                                                                                <  2:1122504/148‑1 (MQ=255)
                                                     aataatAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACg                                                                                              <  2:2774736/148‑1 (MQ=255)
                                                        aataGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAg                                                                                           >  2:657565/1‑148 (MQ=255)
                                                         ataGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGa                                                                                          <  2:1743410/148‑1 (MQ=255)
                                                         ataGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGa                                                                                          >  1:2270699/1‑148 (MQ=255)
                                                          taGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGaa                                                                                         >  2:541648/1‑148 (MQ=255)
                                                           aGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGaaa                                                                                        <  1:2986765/148‑1 (MQ=255)
                                                              gatgaATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAAttt                                                                                     <  1:833351/148‑1 (MQ=255)
                                                                tgaATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATttat                                                                                   <  1:1637622/148‑1 (MQ=255)
                                                                 gaATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATttatt                                                                                  >  1:1288870/1‑148 (MQ=255)
                                                                   aTGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTtattat                                                                                <  1:170812/148‑1 (MQ=255)
                                                                        cAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAAtt                                                                           <  2:2311288/148‑1 (MQ=255)
                                                                        cAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAAtt                                                                           <  1:2797139/148‑1 (MQ=255)
                                                                        cAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAAtt                                                                           <  2:1671376/148‑1 (MQ=255)
                                                                         aaaTCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTc                                                                          <  2:1912284/148‑1 (MQ=255)
                                                                          aaTCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCt                                                                         <  2:1098304/148‑1 (MQ=255)
                                                                            tCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTtg                                                                       <  1:2066081/148‑1 (MQ=255)
                                                                            tCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTtg                                                                       >  2:1584980/1‑148 (MQ=255)
                                                                               aCTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGc                                                                    <  2:1224873/148‑1 (MQ=255)
                                                                               aCTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGc                                                                    <  2:19050/148‑1 (MQ=255)
                                                                                   aaaCACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCAttt                                                                <  1:2342449/148‑1 (MQ=255)
                                                                                     acacGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTAt                                                              >  1:1549639/1‑148 (MQ=255)
                                                                                     acacGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTAt                                                              >  1:1018730/1‑148 (MQ=255)
                                                                                     acacGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTAt                                                              <  1:2452618/148‑1 (MQ=255)
                                                                                      cacGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATg                                                             >  1:1947795/1‑148 (MQ=255)
                                                                                      cacGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATg                                                             >  2:2813921/1‑148 (MQ=255)
                                                                                        cGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCg                                                           <  1:2657257/148‑1 (MQ=255)
                                                                                         gCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGt                                                          >  1:1593862/1‑148 (MQ=255)
                                                                                          cATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGtt                                                         <  1:3100039/148‑1 (MQ=255)
                                                                                           aTTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTc                                                        <  2:2675443/148‑1 (MQ=255)
                                                                                           aTTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTc                                                        <  1:515136/148‑1 (MQ=255)
                                                                                            ttATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCg                                                       >  1:1822887/1‑148 (MQ=255)
                                                                                             tATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGa                                                      >  1:732022/1‑148 (MQ=255)
                                                                                                cGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGaaaa                                                   >  1:3305758/1‑148 (MQ=255)
                                                                                                  aaaGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATc                                                 >  2:3181410/1‑148 (MQ=255)
                                                                                                  aaaGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTGATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATc                                                 <  2:1477195/148‑1 (MQ=255)
                                                                                                    aGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCAt                                               >  2:1178722/1‑148 (MQ=255)
                                                                                                     ggAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATg                                              <  2:2530893/148‑1 (MQ=255)
                                                                                                       agaAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAg                                            >  2:624659/1‑148 (MQ=255)
                                                                                                           aaTCTGTCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATc                                        <  2:2865771/148‑1 (MQ=255)
                                                                                                           aaTCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATc                                        >  2:1304583/1‑148 (MQ=255)
                                                                                                             tCTGGCATTACTACATGGTTTAACTCCTGATATCCTTGATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTa                                      >  2:1425130/1‑148 (MQ=255)
                                                                                                               tGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTaaa                                    <  1:2868275/148‑1 (MQ=255)
                                                                                                                ggCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTaaaa                                   >  1:2236671/1‑148 (MQ=255)
                                                                                                                 gCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAg                                  <  2:249747/148‑1 (MQ=255)
                                                                                                                 gCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAg                                  >  1:1533771/1‑148 (MQ=255)
                                                                                                                 gCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAg                                  <  1:764237/148‑1 (MQ=255)
                                                                                                                  cATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGa                                 >  2:1981136/1‑148 (MQ=255)
                                                                                                                             ggTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAg                      >  1:2943451/1‑148 (MQ=255)
                                                                                                                              gTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAGt                     <  1:1280980/148‑1 (MQ=255)
                                                                                                                                   aCTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAGTATGTc                >  2:1261479/1‑148 (MQ=255)
                                                                                                                                   aCTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACGACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAGTATGTc                <  2:3147900/148‑1 (MQ=255)
                                                                                                                                     tCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAGTATGTCAg              >  1:1013014/1‑148 (MQ=255)
                                                                                                                                     tCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAGTATGTCAg              <  2:1288870/148‑1 (MQ=255)
                                                                                                                                     tCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAGTATGTCAg              >  1:1342051/1‑148 (MQ=255)
                                                                                                                                      ccTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAGTATGTCAGc             <  2:343907/148‑1 (MQ=255)
                                                                                                                                      ccTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAGTATGTCAGc             <  1:1392132/148‑1 (MQ=255)
                                                                                                                                       cTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAGTATGTCAGcc            >  2:2736754/1‑148 (MQ=255)
                                                                                                                                        tGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAGTATGTCAGccc           >  2:877896/1‑148 (MQ=255)
                                                                                                                                          atatCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAGTATGTCAGCCCaa         <  1:3468026/148‑1 (MQ=255)
                                                                                                                                            atCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAGTATGTCAGCCCaaaa       <  2:3332076/148‑1 (MQ=255)
                                                                                                                                             tCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAGTATGTCAGCCCaaaaa      >  1:332159/1‑148 (MQ=255)
                                                                                                                                                 tGATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAGTATGTCAGCCCAAAAAGaaa  >  1:2537541/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTGATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAGTATGTCAGCCCAAAAAGAAA  >  CP009273/2984601‑2984893

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTGATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAGTATGTCAGCCCAAAAAGAAATAT  >  CP009273/2984598‑2984896
                                                                                                                                                     |                                                                                                                                                     
CATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTT                                                                                                                                                       <  SRR19973067.1997255/150‑1 (MQ=60)
 ATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTGA                                                                                                                                                      >  SRR19973067.947996/1‑150 (MQ=60)
 ATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTGA                                                                                                                                                      >  SRR19973067.1420625/1‑150 (MQ=60)
 ATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTGA                                                                                                                                                      >  SRR19973067.1420623/1‑150 (MQ=60)
  TCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTAT                                                                                                                                                     >  SRR19973067.2821704/1‑150 (MQ=60)
  TCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTAT                                                                                                                                                     >  SRR19973067.2821587/1‑150 (MQ=60)
   CACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATA                                                                                                                                                    >  SRR19973067.3167874/1‑150 (MQ=60)
       AGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAAT                                                                                                                                                <  SRR19973067.325410/150‑1 (MQ=60)
       AGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAAT                                                                                                                                                >  SRR19973067.1745609/1‑150 (MQ=60)
       AGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAAT                                                                                                                                                <  SRR19973067.2817770/150‑1 (MQ=60)
         ACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATAT                                                                                                                                              <  SRR19973067.2988991/150‑1 (MQ=60)
          CACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTGATAGAATATA                                                                                                                                             >  SRR19973067.890483/1‑150 (MQ=60)
             ATCACAGAGGAATATGATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGC                                                                                                                                          >  SRR19973067.1765486/1‑150 (MQ=60)
             ATCACAGAGGAATATGATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGC                                                                                                                                          >  SRR19973067.1764703/1‑150 (MQ=60)
              TCACAGAGGAATATGATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCA                                                                                                                                         >  SRR19973067.2678427/1‑150 (MQ=60)
                ACAGAGGAATATGATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATA                                                                                                                                       >  SRR19973067.1801180/1‑150 (MQ=60)
                 CAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATAT                                                                                                                                      <  SRR19973067.1994001/150‑1 (MQ=60)
                 CAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATAT                                                                                                                                      <  SRR19973067.667508/150‑1 (MQ=60)
                 CAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATAT                                                                                                                                      <  SRR19973067.3083701/150‑1 (MQ=60)
                    attAATATTATGATCACAGAAACAATTGAAATATTAAATAATAGTGATGTATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCA                                                                                                                                   <  SRR19973067.2808027/147‑1 (MQ=60)
                    AGTAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGTCAAATCAACTAAAACACTCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCA                                                                                                                                   <  SRR19973067.29776/150‑1 (MQ=60)
                     GGATTATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCAT                                                                                                                                  <  SRR19973067.1342668/150‑1 (MQ=60)
                      GAATATTATGATCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATT                                                                                                                                 <  SRR19973067.613878/150‑1 (MQ=60)
                       AATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTC                                                                                                                                <  SRR19973067.1638801/150‑1 (MQ=60)
                       AATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTC                                                                                                                                >  SRR19973067.2182240/1‑150 (MQ=60)
                       AATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTC                                                                                                                                <  SRR19973067.2648518/150‑1 (MQ=60)
                         TATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGA                                                                                                                              <  SRR19973067.324032/150‑1 (MQ=60)
                          ATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGAC                                                                                                                             >  SRR19973067.1556141/1‑150 (MQ=60)
                             ATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTAC                                                                                                                          <  SRR19973067.2014907/150‑1 (MQ=60)
                                 GCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATG                                                                                                                      <  SRR19973067.2257139/150‑1 (MQ=60)
                                 GCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTGATAGAATATATGCATATGCATTCGACTACCATG                                                                                                                      <  SRR19973067.217288/150‑1 (MQ=60)
                                 GCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTGATAGAATATATGCATATGCATTCGACTACCATG                                                                                                                      <  SRR19973067.217108/150‑1 (MQ=60)
                                   ACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAA                                                                                                                    >  SRR19973067.569838/1‑150 (MQ=60)
                                   ACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAA                                                                                                                    >  SRR19973067.569861/1‑150 (MQ=60)
                                      GAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAG                                                                                                                 >  SRR19973067.350289/1‑150 (MQ=60)
                                       AAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGG                                                                                                                <  SRR19973067.1428222/150‑1 (MQ=60)
                                        AACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGG                                                                                                               >  SRR19973067.2331327/1‑150 (MQ=60)
                                        AACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGG                                                                                                               >  SRR19973067.1471683/1‑150 (MQ=60)
                                         ACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGT                                                                                                              >  SRR19973067.668524/1‑150 (MQ=60)
                                         ACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGT                                                                                                              >  SRR19973067.667978/1‑150 (MQ=60)
                                           AATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAA                                                                                                            <  SRR19973067.3371857/150‑1 (MQ=60)
                                           AATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAA                                                                                                            <  SRR19973067.3371859/150‑1 (MQ=60)
                                               GAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATA                                                                                                        >  SRR19973067.1755314/1‑150 (MQ=60)
                                               GAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATA                                                                                                        >  SRR19973067.1755293/1‑150 (MQ=60)
                                                AAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGACTATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAA                                                                                                       >  SRR19973067.636377/1‑150 (MQ=60)
                                                AAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAA                                                                                                       >  SRR19973067.636513/1‑150 (MQ=60)
                                                    ATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGA                                                                                                   <  SRR19973067.854038/150‑1 (MQ=60)
                                                     TTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGAC                                                                                                  <  SRR19973067.1772956/150‑1 (MQ=60)
                                                     TTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGAC                                                                                                  <  SRR19973067.2097522/150‑1 (MQ=60)
                                                         ATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAG                                                                                              >  SRR19973067.2219058/1‑150 (MQ=60)
                                                          TAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGA                                                                                             >  SRR19973067.2287019/1‑150 (MQ=60)
                                                          TAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGA                                                                                             >  SRR19973067.2285741/1‑150 (MQ=60)
                                                              AGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATT                                                                                         <  SRR19973067.3005657/150‑1 (MQ=60)
                                                              AGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATT                                                                                         >  SRR19973067.3455847/1‑150 (MQ=60)
                                                                TGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTGATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTA                                                                                       <  SRR19973067.1665602/150‑1 (MQ=60)
                                                                 GATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTAT                                                                                      <  SRR19973067.839023/150‑1 (MQ=60)
                                                                 GATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTAT                                                                                      <  SRR19973067.2237721/150‑1 (MQ=60)
                                                                  ATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATT                                                                                     >  SRR19973067.1297666/1‑150 (MQ=60)
                                                                   TGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTA                                                                                    <  SRR19973067.1648890/150‑1 (MQ=60)
                                                                     AATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATA                                                                                  <  SRR19973067.1568443/150‑1 (MQ=60)
                                                                     AATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACCCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATA                                                                                  <  SRR19973067.1568474/150‑1 (MQ=60)
                                                                      ATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAA                                                                                 <  SRR19973067.172198/150‑1 (MQ=60)
                                                                          GCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTC                                                                             <  SRR19973067.1920186/150‑1 (MQ=60)
                                                                           CAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCT                                                                            >  SRR19973067.1290581/1‑150 (MQ=60)
                                                                           CAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCT                                                                            <  SRR19973067.2815178/150‑1 (MQ=60)
                                                                               TCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTG                                                                        <  SRR19973067.2079821/150‑1 (MQ=60)
                                                                                CAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGC                                                                       >  SRR19973067.116100/1‑150 (MQ=60)
                                                                                    TAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTT                                                                   >  SRR19973067.759163/1‑150 (MQ=60)
                                                                                      AAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTAT                                                                 <  SRR19973067.2357768/150‑1 (MQ=60)
                                                                                      AAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTAT                                                                 >  SRR19973067.1025725/1‑150 (MQ=60)
                                                                                      AAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTAT                                                                 >  SRR19973067.1560044/1‑150 (MQ=60)
                                                                                      AAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTAT                                                                 >  SRR19973067.1560188/1‑150 (MQ=60)
                                                                                       AACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATG                                                                >  SRR19973067.1857960/1‑150 (MQ=60)
                                                                                       AACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATG                                                                >  SRR19973067.151700/1‑150 (MQ=60)
                                                                                       AACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATG                                                                >  SRR19973067.1961067/1‑150 (MQ=60)
                                                                                       AACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATG                                                                <  SRR19973067.1361138/150‑1 (MQ=60)
                                                                                        ACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGC                                                               <  SRR19973067.2468740/150‑1 (MQ=60)
                                                                                          ACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGT                                                             >  SRR19973067.1604786/1‑150 (MQ=60)
                                                                                           CGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTT                                                            <  SRR19973067.2674364/150‑1 (MQ=60)
                                                                                             CATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCG                                                          <  SRR19973067.1428171/150‑1 (MQ=60)
                                                                                             CATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCG                                                          <  SRR19973067.1428118/150‑1 (MQ=60)
                                                                                             CATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCG                                                          >  SRR19973067.3132814/1‑150 (MQ=60)
                                                                                             CATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCG                                                          <  SRR19973067.3119768/150‑1 (MQ=60)
                                                                                             CATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCG                                                          >  SRR19973067.1835250/1‑150 (MQ=60)
                                                                                              ATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGA                                                         >  SRR19973067.2532299/1‑150 (MQ=60)
                                                                                              ATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGA                                                         <  SRR19973067.518632/150‑1 (MQ=60)
                                                                                              ATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGA                                                         >  SRR19973067.737106/1‑150 (MQ=60)
                                                                                                 ATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAA                                                      >  SRR19973067.1315107/1‑150 (MQ=60)
                                                                                                 ATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAA                                                      >  SRR19973067.3327118/1‑150 (MQ=60)
                                                                                                 ATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAA                                                      >  SRR19973067.3326612/1‑150 (MQ=60)
                                                                                                     AAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCAT                                                  <  SRR19973067.1453740/150‑1 (MQ=60)
                                                                                                       AGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGA                                                <  SRR19973067.2465354/150‑1 (MQ=60)
                                                                                                            AAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATC                                           >  SRR19973067.2095079/1‑150 (MQ=60)
                                                                                                              AATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTA                                         >  SRR19973067.1240459/1‑150 (MQ=60)
                                                                                                                 CTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAA                                      >  SRR19973067.1369391/1‑150 (MQ=60)
                                                                                                                 CTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAA                                      >  SRR19973067.2251549/1‑150 (MQ=60)
                                                                                                                  TNGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAG                                     >  SRR19973067.1544148/1‑150 (MQ=60)
                                                                                                                  TGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAG                                     <  SRR19973067.2886640/150‑1 (MQ=60)
                                                                                                                  TGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAG                                     >  SRR19973067.1544570/1‑150 (MQ=60)
                                                                                                                    GCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGAT                                   <  SRR19973067.769547/150‑1 (MQ=60)
                                                                                                                          CTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCA                             <  SRR19973067.1635625/150‑1 (MQ=60)
                                                                                                                           TACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCAT                            <  SRR19973067.1505487/150‑1 (MQ=60)
                                                                                                                           TACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCAT                            >  SRR19973067.1555644/1‑150 (MQ=60)
                                                                                                                           TACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCAT                            <  SRR19973067.2659565/150‑1 (MQ=60)
                                                                                                                              ATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAG                         >  SRR19973067.2962169/1‑150 (MQ=60)
                                                                                                                              ATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAG                         >  SRR19973067.1968133/1‑150 (MQ=60)
                                                                                                                              ATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAG                         >  SRR19973067.2962069/1‑150 (MQ=60)
                                                                                                                              ATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAG                         >  SRR19973067.2962099/1‑150 (MQ=60)
                                                                                                                              ATGGATTAACTCCTGATATCCTTTATAGAATATATGCATATGCAGTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAG                         <  SRR19973067.617275/150‑1 (MQ=60)
                                                                                                                               TGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAGT                        >  SRR19973067.3313653/1‑150 (MQ=60)
                                                                                                                               TGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAGT                        >  SRR19973067.3312985/1‑150 (MQ=60)
                                                                                                                                GGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAGTA                       >  SRR19973067.3156430/1‑150 (MQ=60)
                                                                                                                                 GTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAGTAT                      <  SRR19973067.1289707/150‑1 (MQ=60)
                                                                                                                                    TAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAGTATGTC                   <  SRR19973067.2877383/150‑1 (MQ=60)
                                                                                                                                      ACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAGTATGTCAG                 >  SRR19973067.1351228/1‑150 (MQ=60)
                                                                                                                                      ACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAGTATGTCAG                 >  SRR19973067.1019980/1‑150 (MQ=60)
                                                                                                                                      ACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAGTATGTCAG                 >  SRR19973067.1019972/1‑150 (MQ=60)
                                                                                                                                       CTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAGTATGTCAGC                <  SRR19973067.2008291/150‑1 (MQ=60)
                                                                                                                                       CTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAGTATGTCAGC                <  SRR19973067.776677/150‑1 (MQ=60)
                                                                                                                                         CCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAGTATGTCAGCCC              <  SRR19973067.1401593/150‑1 (MQ=60)
                                                                                                                                          CTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAGTATGTCAGCCCA             >  SRR19973067.2421139/1‑150 (MQ=60)
                                                                                                                                          CTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAGTATGTCAGCCCA             <  SRR19973067.3405495/150‑1 (MQ=60)
                                                                                                                                            GATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAGTATGTCAGCCCAAA           >  SRR19973067.363535/1‑150 (MQ=60)
                                                                                                                                             ATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAGTATGTCAGCCCAAAA          <  SRR19973067.3490066/150‑1 (MQ=60)
                                                                                                                                              TATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAGTATGTCAGCCCAAAAA         >  SRR19973067.334023/1‑150 (MQ=60)
                                                                                                                                              TATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAGTATGTCAGCCCAAAAA         >  SRR19973067.334429/1‑150 (MQ=60)
                                                                                                                                                TCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAGTATGTCAGCCCAAAAAGA       <  SRR19973067.76227/150‑1 (MQ=60)
                                                                                                                                                  CTTGATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAGTATGTCAGCCCAAAAAGAAA     >  SRR19973067.2556422/1‑150 (MQ=60)
                                                                                                                                                  CTTGATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAGTATGTCAGCCCAAAAAGAAA     >  SRR19973067.2554412/1‑150 (MQ=60)
                                                                                                                                                  CTTGATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAGTATGTCAGCCCAAAAAGAAA     >  SRR19973067.2554155/1‑150 (MQ=60)
                                                                                                                                                    TTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAGTATGTCAGCCCAAAAAGAAATA   <  SRR19973067.709851/150‑1 (MQ=60)
                                                                                                                                                     TATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAGTATGTCAGCCCAAAAAGAAATAT  <  SRR19973067.3288686/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
CATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTGATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAGTATGTCAGCCCAAAAAGAAATAT  >  CP009273/2984598‑2984896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑