Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F361 I0 R1 1353 250.7 8230166 96.0% 7900959 148.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,984,747 G→T 100% D41Y (GAT→TAT)  ygeG → SycD‑like chaperone family TPR‑repeat‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,984,7470GT99.5% 694.1 / ‑4.3 208D41Y (GAT→TAT) ygeGSycD‑like chaperone family TPR‑repeat‑containing protein
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (96/111);  total (97/111)
Fisher's exact test for biased strand distribution p-value = 4.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 209 total aligned reads displayed.

CACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTGATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAGTATGTCAGCCCAAAAAGAA  >  CP009273/2984601‑2984892
                                                                                                                                                  |                                                                                                                                                 
cACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTAt                                                                                                                                                 <  1:482637/149‑1 (MQ=255)
   gagaCACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGa                                                                                                                                              <  2:401852/149‑1 (MQ=255)
   gagaCACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGa                                                                                                                                              >  1:3369489/1‑149 (MQ=255)
    agaCACCTCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGaa                                                                                                                                             <  2:3680133/149‑1 (MQ=255)
    agaCACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGaa                                                                                                                                             >  1:3682053/1‑149 (MQ=255)
     gaCACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAat                                                                                                                                            <  2:3947101/149‑1 (MQ=255)
        acaTCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAatata                                                                                                                                         >  2:1217756/1‑149 (MQ=255)
         caTCACAGAGGAATATGATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAatatat                                                                                                                                        >  2:187851/1‑149 (MQ=255)
          aTCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATatatg                                                                                                                                       <  2:3789946/149‑1 (MQ=255)
           tCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATatatgc                                                                                                                                      <  1:3630404/149‑1 (MQ=255)
              caGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATatatgcata                                                                                                                                   <  2:2261675/149‑1 (MQ=255)
                 agGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATatatgcatatgc                                                                                                                                >  1:1033512/1‑149 (MQ=255)
                 agGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATatatgcatatgc                                                                                                                                >  1:2925328/1‑149 (MQ=255)
                 agGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATatatgcatatgc                                                                                                                                <  1:2455898/149‑1 (MQ=255)
                   gAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATatgcatatgcat                                                                                                                              <  1:1009947/149‑1 (MQ=255)
                    aaTATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCAtt                                                                                                                             <  1:3042503/149‑1 (MQ=255)
                     atatTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTc                                                                                                                            <  2:1335099/149‑1 (MQ=255)
                       attatGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGa                                                                                                                          <  1:946714/149‑1 (MQ=255)
                        ttatGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGAc                                                                                                                         <  1:3635878/149‑1 (MQ=255)
                        ttatGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGAc                                                                                                                         <  1:1638063/149‑1 (MQ=255)
                         tatGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACt                                                                                                                        <  1:1909137/149‑1 (MQ=255)
                                 caGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGaa                                                                                                                >  1:2715284/1‑149 (MQ=255)
                                     aaCAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAgg                                                                                                            <  2:2379516/149‑1 (MQ=255)
                                          ttGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAat                                                                                                       >  1:303483/1‑149 (MQ=255)
                                           tGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAata                                                                                                      <  2:262613/149‑1 (MQ=255)
                                           tGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAata                                                                                                      >  1:1103349/1‑149 (MQ=255)
                                              aaTATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATaa                                                                                                   >  2:1556434/1‑149 (MQ=255)
                                              aaTATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATaa                                                                                                   <  1:2032313/149‑1 (MQ=255)
                                              aaTATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATaa                                                                                                   >  2:1788173/1‑149 (MQ=255)
                                                  ttCAATAATAGTGATGAATGGTCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGa                                                                                               <  2:2938122/149‑1 (MQ=255)
                                                   tCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGAc                                                                                              <  2:2095427/149‑1 (MQ=255)
                                                     aataatAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGc                                                                                            >  2:3765863/1‑149 (MQ=255)
                                                     aataatAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGc                                                                                            >  1:1143710/1‑149 (MQ=255)
                                                     aataatAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGc                                                                                            <  1:2375756/149‑1 (MQ=255)
                                                     aataatAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGc                                                                                            <  2:2453327/149‑1 (MQ=255)
                                                     aataatAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGc                                                                                            <  2:384431/149‑1 (MQ=255)
                                                      ataataGTTATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCa                                                                                           <  1:4048230/149‑1 (MQ=255)
                                                       taataGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAg                                                                                          <  2:3170086/149‑1 (MQ=255)
                                                        aataGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGa                                                                                         >  2:1413425/1‑149 (MQ=255)
                                                         ataGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGaa                                                                                        >  1:1902563/1‑149 (MQ=255)
                                                          taGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGaaa                                                                                       >  2:3480687/1‑149 (MQ=255)
                                                           aGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAAt                                                                                      <  1:857164/149‑1 (MQ=255)
                                                           aGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTGATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAAt                                                                                      >  2:2805731/1‑149 (MQ=255)
                                                               atgaATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATttat                                                                                  <  1:681060/149‑1 (MQ=255)
                                                                tgaATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATttatt                                                                                 >  2:2597705/1‑149 (MQ=255)
                                                                      ggCAAATCAACTAAAACACTCATTATCTAAAGGAGAAAATCTGGAATTACTACATTGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCTACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAAt                                                                           <  1:1429433/149‑1 (MQ=255)
                                                                          aaTCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCtt                                                                       >  2:455129/1‑149 (MQ=255)
                                                                               aCTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCa                                                                  <  1:3238153/149‑1 (MQ=255)
                                                                               aCTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCa                                                                  <  2:1129331/149‑1 (MQ=255)
                                                                                  aaaaCACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCAttt                                                               >  2:1812349/1‑149 (MQ=255)
                                                                                     acacGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATg                                                            >  2:1665876/1‑149 (MQ=255)
                                                                                     acacGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATg                                                            <  2:2206472/149‑1 (MQ=255)
                                                                                     acacGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATg                                                            >  2:2318405/1‑149 (MQ=255)
                                                                                      cacGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGc                                                           >  1:573447/1‑149 (MQ=255)
                                                                                      cacGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGc                                                           >  1:229213/1‑149 (MQ=255)
                                                                                      cacGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGc                                                           <  1:154027/149‑1 (MQ=255)
                                                                                      cacGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGc                                                           <  1:1831663/149‑1 (MQ=255)
                                                                                        cGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGt                                                         >  2:1494951/1‑149 (MQ=255)
                                                                                         gCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGtt                                                        >  1:245709/1‑149 (MQ=255)
                                                                                         gCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGtt                                                        <  1:1771372/149‑1 (MQ=255)
                                                                                          cATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTc                                                       <  1:1284914/149‑1 (MQ=255)
                                                                                           aTTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCg                                                      <  1:3614068/149‑1 (MQ=255)
                                                                                           aTTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCg                                                      <  1:2041477/149‑1 (MQ=255)
                                                                                           aTTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCg                                                      >  1:3397095/1‑149 (MQ=255)
                                                                                             tATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGaa                                                    >  2:2683186/1‑149 (MQ=255)
                                                                                              aTCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGaaa                                                   <  1:3426462/149‑1 (MQ=255)
                                                                                               tctaaaGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATAGGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGaaaa                                                  <  2:2111489/146‑1 (MQ=255)
                                                                                               tCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGaaaa                                                  <  2:1918807/149‑1 (MQ=255)
                                                                                               tCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGaaaa                                                  >  2:686366/1‑149 (MQ=255)
                                                                                                 gAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATc                                                <  2:3463052/149‑1 (MQ=255)
                                                                                                  aaaGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCa                                               >  1:1800241/1‑149 (MQ=255)
                                                                                                    atgagaAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATg                                             <  2:1515843/147‑1 (MQ=255)
                                                                                                       agaAAATCTGGCATTACTACATGGTTTAACTCCTTATATACTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGTTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGt                                          <  1:3038608/149‑1 (MQ=255)
                                                                                                       agaAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGt                                          <  1:3488446/149‑1 (MQ=255)
                                                                                                       agaAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGt                                          >  2:2244690/1‑149 (MQ=255)
                                                                                                       agaAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGt                                          >  1:510895/1‑149 (MQ=255)
                                                                                                        gaAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTa                                         <  2:1932420/149‑1 (MQ=255)
                                                                                                          aaaTCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATc                                       <  1:2425817/149‑1 (MQ=255)
                                                                                                           aaTCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCt                                      <  1:2131126/149‑1 (MQ=255)
                                                                                                             tCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTaa                                    >  2:317919/1‑149 (MQ=255)
                                                                                                               tGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTaaaa                                  >  1:2309591/1‑149 (MQ=255)
                                                                                                                   aTTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGAtt                              <  1:4019176/149‑1 (MQ=255)
                                                                                                                    ttACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGAttt                             <  1:742554/149‑1 (MQ=255)
                                                                                                                    ttACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGAttt                             >  1:1263985/1‑149 (MQ=255)
                                                                                                                    ttACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGAttt                             <  1:3428589/149‑1 (MQ=255)
                                                                                                                    ttACTACATGGTTTAACTCCTGATATCATTTTTAGAATATATGCATATGCATTCGACTACCATGAAAAGTGTAATATAACGTACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGATAATCATGAGTATCTAAAAGAttt                             <  1:64035/149‑1 (MQ=255)
                                                                                                                      actacATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTg                           <  1:1829170/149‑1 (MQ=255)
                                                                                                                       ctacATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGc                          <  1:3413841/149‑1 (MQ=255)
                                                                                                                       ctacATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGc                          <  1:1363171/149‑1 (MQ=255)
                                                                                                                            tGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAg                     >  1:2693069/1‑149 (MQ=255)
                                                                                                                             ggTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAGt                    <  2:2481732/149‑1 (MQ=255)
                                                                                                                              gTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAGTa                   >  1:1314269/1‑149 (MQ=255)
                                                                                                                                ttAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAGTATg                 <  1:1217756/149‑1 (MQ=255)
                                                                                                                                  aaCTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAGTATGTc               <  2:1143710/149‑1 (MQ=255)
                                                                                                                                   aCTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATTAGTATGTCa              <  2:429178/149‑1 (MQ=255)
                                                                                                                                     tCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAGTATGTCAGc            <  2:29812/149‑1 (MQ=255)
                                                                                                                                             tCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAGTATGTCAGCCCAAAAAg    >  1:3880940/1‑149 (MQ=255)
                                                                                                                                             tCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAGTATGTCAGCCCAAAAAg    >  1:2614263/1‑149 (MQ=255)
                                                                                                                                             tCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGAATCAGTATGTAAGCCCAAAAAg    >  2:3583739/1‑149 (MQ=255)
                                                                                                                                              ccTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAGTATGTCAGCCCAAAAAGa   <  2:1351244/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTGATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAGTATGTCAGCCCAAAAAGAA  >  CP009273/2984601‑2984892

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTGATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAGTATGTCAGCCCAAAAAGAAAT  >  CP009273/2984599‑2984894
                                                                                                                                                    |                                                                                                                                                   
ATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTA                                                                                                                                                    >  SRR19973068.2109065/1‑150 (MQ=60)
 TCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTAT                                                                                                                                                   <  SRR19973068.1043389/150‑1 (MQ=60)
  CACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATA                                                                                                                                                  <  SRR19973068.486031/150‑1 (MQ=60)
  CACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATA                                                                                                                                                  <  SRR19973068.486139/150‑1 (MQ=60)
    CGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGA                                                                                                                                                >  SRR19973068.1327122/1‑150 (MQ=60)
    CGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGA                                                                                                                                                >  SRR19973068.30222/1‑150 (MQ=60)
    CGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGA                                                                                                                                                >  SRR19973068.3389961/1‑150 (MQ=60)
    CGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGA                                                                                                                                                >  SRR19973068.736331/1‑150 (MQ=60)
     GAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAA                                                                                                                                               >  SRR19973068.3703780/1‑150 (MQ=60)
     GAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAA                                                                                                                                               >  SRR19973068.3704274/1‑150 (MQ=60)
          ACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATAT                                                                                                                                          >  SRR19973068.4064952/1‑150 (MQ=60)
             TCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCA                                                                                                                                       <  SRR19973068.3652409/150‑1 (MQ=60)
                  GAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGC                                                                                                                                  >  SRR19973068.1040161/1‑150 (MQ=60)
                  GAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGC                                                                                                                                  >  SRR19973068.2943500/1‑150 (MQ=60)
                   AGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCA                                                                                                                                 <  SRR19973068.2471560/150‑1 (MQ=60)
                    GGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCAT                                                                                                                                <  SRR19973068.95121/150‑1 (MQ=60)
                     GAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATT                                                                                                                               <  SRR19973068.2472690/150‑1 (MQ=60)
                     GAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATT                                                                                                                               <  SRR19973068.1553190/150‑1 (MQ=60)
                     GAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATT                                                                                                                               <  SRR19973068.1016522/150‑1 (MQ=60)
                     GAATATTATGAGCACAGAAACAATTGAAATATCCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATT                                                                                                                               <  SRR19973068.2473564/150‑1 (MQ=60)
                      AATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTC                                                                                                                              <  SRR19973068.3061162/150‑1 (MQ=60)
                         ATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGAC                                                                                                                           <  SRR19973068.953054/150‑1 (MQ=60)
                         ATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGAC                                                                                                                           <  SRR19973068.2838757/150‑1 (MQ=60)
                          TTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACT                                                                                                                          <  SRR19973068.1648726/150‑1 (MQ=60)
                          TTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACT                                                                                                                          <  SRR19973068.3657910/150‑1 (MQ=60)
                          TTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACT                                                                                                                          <  SRR19973068.3656779/150‑1 (MQ=60)
                           TATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTA                                                                                                                         <  SRR19973068.3446761/150‑1 (MQ=60)
                           TATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTA                                                                                                                         <  SRR19973068.1921812/150‑1 (MQ=60)
                           TATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTA                                                                                                                         <  SRR19973068.1921750/150‑1 (MQ=60)
                                 CACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGA                                                                                                                   <  SRR19973068.522101/150‑1 (MQ=60)
                                  ACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAA                                                                                                                  >  SRR19973068.2732092/1‑150 (MQ=60)
                                           ATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAAT                                                                                                         >  SRR19973068.305501/1‑150 (MQ=60)
                                            TTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATA                                                                                                        >  SRR19973068.1110694/1‑150 (MQ=60)
                                            TTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATA                                                                                                        >  SRR19973068.1114203/1‑150 (MQ=60)
                                               AAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAA                                                                                                     <  SRR19973068.4033319/150‑1 (MQ=60)
                                                AATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAAC                                                                                                    <  SRR19973068.2045617/150‑1 (MQ=60)
                                                AATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAAC                                                                                                    <  SRR19973068.2045720/150‑1 (MQ=60)
                                                 ATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACG                                                                                                   >  SRR19973068.3109564/1‑150 (MQ=60)
                                                 ATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACG                                                                                                   <  SRR19973068.2677655/150‑1 (MQ=60)
                                                      CAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGC                                                                                              >  SRR19973068.1151362/1‑150 (MQ=60)
                                                       AATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCA                                                                                             <  SRR19973068.2391141/150‑1 (MQ=60)
                                                       AATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCA                                                                                             <  SRR19973068.2390932/150‑1 (MQ=60)
                                                        ATAATAGTTATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAG                                                                                            <  SRR19973068.4072600/150‑1 (MQ=60)
                                                        ATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAG                                                                                            >  SRR19973068.3416723/1‑150 (MQ=60)
                                                          AATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAA                                                                                          >  SRR19973068.1915202/1‑150 (MQ=60)
                                                            TAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAAT                                                                                        >  SRR19973068.4096552/1‑150 (MQ=60)
                                                             AGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATT                                                                                       <  SRR19973068.862985/150‑1 (MQ=60)
                                                               TGATGAATGGTCAAATCAACTAAAACACGCATTATCGAAAGGATAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCTACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTA                                                                                     <  SRR19973068.118688/150‑1 (MQ=60)
                                                               TGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTA                                                                                     >  SRR19973068.1689252/1‑150 (MQ=60)
                                                                 ATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATT                                                                                   <  SRR19973068.685381/150‑1 (MQ=60)
                                                                 ATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATT                                                                                   >  SRR19973068.3824911/1‑150 (MQ=60)
                                                                 ATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATT                                                                                   <  SRR19973068.996736/150‑1 (MQ=60)
                                                                 ATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATT                                                                                   <  SRR19973068.1038684/150‑1 (MQ=60)
                                                                   GAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTAT                                                                                 <  SRR19973068.1480101/150‑1 (MQ=60)
                                                                        GGCAAATCAACTAAAACACTCATTATCTAAAGGAGAAAATCTGGAATTACTACATTGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCTACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATT                                                                            <  SRR19973068.1438771/150‑1 (MQ=60)
                                                                        GGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATT                                                                            <  SRR19973068.66717/150‑1 (MQ=60)
                                                                          CAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCT                                                                          <  SRR19973068.310443/150‑1 (MQ=60)
                                                                          CAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCT                                                                          <  SRR19973068.209453/150‑1 (MQ=60)
                                                                             ATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGT                                                                       >  SRR19973068.624610/1‑150 (MQ=60)
                                                                                AACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCA                                                                    >  SRR19973068.1837048/1‑150 (MQ=60)
                                                                                 ACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCAT                                                                   <  SRR19973068.3258056/150‑1 (MQ=60)
                                                                                  CTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATT                                                                  >  SRR19973068.3716613/1‑150 (MQ=60)
                                                                                   TAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTT                                                                 >  SRR19973068.1841631/1‑150 (MQ=60)
                                                                                     AAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTAT                                                               >  SRR19973068.3561729/1‑150 (MQ=60)
                                                                                       ACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGC                                                             >  SRR19973068.577373/1‑150 (MQ=60)
                                                                                       ACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGC                                                             >  SRR19973068.230913/1‑150 (MQ=60)
                                                                                       ACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGAATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGC                                                             >  SRR19973068.841921/1‑150 (MQ=60)
                                                                                        CACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCG                                                            <  SRR19973068.155330/150‑1 (MQ=60)
                                                                                        CACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCG                                                            <  SRR19973068.1843742/150‑1 (MQ=60)
                                                                                          CGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTT                                                          >  SRR19973068.247497/1‑150 (MQ=60)
                                                                                           GCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTC                                                         <  SRR19973068.1782857/150‑1 (MQ=60)
                                                                                            CATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCG                                                        <  SRR19973068.1293354/150‑1 (MQ=60)
                                                                                            CATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCG                                                        >  SRR19973068.3417662/1‑150 (MQ=60)
                                                                                            CATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCG                                                        <  SRR19973068.1293361/150‑1 (MQ=60)
                                                                                             ATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGA                                                       <  SRR19973068.3637327/150‑1 (MQ=60)
                                                                                             ATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGA                                                       <  SRR19973068.3636009/150‑1 (MQ=60)
                                                                                             ATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGA                                                       <  SRR19973068.2054807/150‑1 (MQ=60)
                                                                                                ATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAA                                                    <  SRR19973068.1911422/150‑1 (MQ=60)
                                                                                                ATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAA                                                    <  SRR19973068.3447113/150‑1 (MQ=60)
                                                                                                  CGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATC                                                  >  SRR19973068.1978619/1‑150 (MQ=60)
                                                                                                   GAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCA                                                 >  SRR19973068.1730823/1‑150 (MQ=60)
                                                                                                   GAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCA                                                 >  SRR19973068.1812033/1‑150 (MQ=60)
                                                                                                   GAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCA                                                 >  SRR19973068.1812020/1‑150 (MQ=60)
                                                                                                      AGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGA                                              >  SRR19973068.3499753/1‑150 (MQ=60)
                                                                                                        GAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGT                                            >  SRR19973068.514448/1‑150 (MQ=60)
                                                                                                         AGAAAATCTGGCATTACTACATGGTTTAACTCCTTATATACTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGTTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTA                                           <  SRR19973068.3057257/150‑1 (MQ=60)
                                                                                                         AGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTA                                           <  SRR19973068.3509638/150‑1 (MQ=60)
                                                                                                         AGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTA                                           <  SRR19973068.3056219/150‑1 (MQ=60)
                                                                                                         AGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTA                                           <  SRR19973068.3057261/150‑1 (MQ=60)
                                                                                                            AAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCT                                        <  SRR19973068.2441208/150‑1 (MQ=60)
                                                                                                             AATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTA                                       <  SRR19973068.2145062/150‑1 (MQ=60)
                                                                                                                CTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAA                                    >  SRR19973068.2324600/1‑150 (MQ=60)
                                                                                                                CTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAA                                    >  SRR19973068.2324503/1‑150 (MQ=60)
                                                                                                                CTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAA                                    >  SRR19973068.2324500/1‑150 (MQ=60)
                                                                                                                CTGGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAA                                    >  SRR19973068.1066215/1‑150 (MQ=60)
                                                                                                                  GGCATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGA                                  <  SRR19973068.1315185/150‑1 (MQ=60)
                                                                                                                    CATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATT                                <  SRR19973068.23533/150‑1 (MQ=60)
                                                                                                                     ATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTT                               >  SRR19973068.1272333/1‑150 (MQ=60)
                                                                                                                     ATTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTT                               <  SRR19973068.4043433/150‑1 (MQ=60)
                                                                                                                      TTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTT                              <  SRR19973068.747505/150‑1 (MQ=60)
                                                                                                                      TTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTT                              <  SRR19973068.3449247/150‑1 (MQ=60)
                                                                                                                      TTACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTT                              <  SRR19973068.3449242/150‑1 (MQ=60)
                                                                                                                      TTACTACATGGTTTAACTCCTGATATCATTTTTAGAATATATGCATATGCATTCGACTACCATGAAAAGTGTAATATAACGTACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGATAATCATGAGTATCTAAAAGATTTT                              <  SRR19973068.64771/150‑1 (MQ=60)
                                                                                                                       TACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTG                             >  SRR19973068.2168398/1‑150 (MQ=60)
                                                                                                                        ACTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGC                            <  SRR19973068.1841225/150‑1 (MQ=60)
                                                                                                                         CTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCA                           <  SRR19973068.1371905/150‑1 (MQ=60)
                                                                                                                         CTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCA                           <  SRR19973068.3328372/150‑1 (MQ=60)
                                                                                                                         CTACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCA                           <  SRR19973068.3434453/150‑1 (MQ=60)
                                                                                                                           ACATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATC                         >  SRR19973068.1237746/1‑150 (MQ=60)
                                                                                                                             ATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAG                       >  SRR19973068.2709817/1‑150 (MQ=60)
                                                                                                                             ATGGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAG                       >  SRR19973068.2709814/1‑150 (MQ=60)
                                                                                                                               GGTTTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAGTA                     >  SRR19973068.1322858/1‑150 (MQ=60)
                                                                                                                                  TTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAGTATGT                  <  SRR19973068.1225860/150‑1 (MQ=60)
                                                                                                                                  TTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAGTATGT                  >  SRR19973068.3430262/1‑150 (MQ=60)
                                                                                                                                  TTAACTCCTGATATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAGTATGT                  <  SRR19973068.2443202/150‑1 (MQ=60)
                                                                                                                                              ATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAGTATGTCAGCCCAAAAAG      >  SRR19973068.2630729/1‑150 (MQ=60)
                                                                                                                                              ATCCTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAGTATGTCAGCCCAAAAAG      >  SRR19973068.3904385/1‑150 (MQ=60)
                                                                                                                                                 CTTTATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAGTATGTCAGCCCAAAAAGAAA   <  SRR19973068.1406992/150‑1 (MQ=60)
                                                                                                                                                  TTTATAGAATATATGCATATTCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCGTCAGTATGTCAGCCCAAAAAGAAAT  <  SRR19973068.3527759/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                   
ATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAATCAACTAAAACACGCATTATCGAAAGGAGAAAATCTGGCATTACTACATGGTTTAACTCCTGATATCCTTGATAGAATATATGCATATGCATTCGACTACCATGAAAAGGGTAATATAACGGACGCAGAAATTTATTATAAATTCTTGTGCATTTATGCGTTCGAAAATCATGAGTATCTAAAAGATTTTGCATCAGTATGTCAGCCCAAAAAGAAAT  >  CP009273/2984599‑2984894

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑