Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,058,769 C→T 100% I203I (ATC→ATT)  scpC → propionyl‑CoA:succinate CoA transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,058,7690CT97.8% 738.2 / 4.9 227I203I (ATC→ATT) scpCpropionyl‑CoA:succinate CoA transferase
Reads supporting (aligned to +/- strand):  ref base C (2/3);  new base T (111/111);  total (113/114)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 227 total aligned reads displayed.

AGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCG  >  CP009273/3058624‑3058913
                                                                                                                                                 |                                                                                                                                                
agaaAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGa                                                                                                                                                >  1:2368387/1‑148 (MQ=255)
 gaaAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCGATGTGCAAATTGAt                                                                                                                                               >  2:3236776/1‑148 (MQ=255)
  aaaGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATc                                                                                                                                              >  2:1677937/1‑148 (MQ=255)
      tGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGaa                                                                                                                                          >  1:2031451/1‑148 (MQ=255)
      tGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGaa                                                                                                                                          >  1:2031537/1‑148 (MQ=255)
         tCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGaaaaa                                                                                                                                       <  1:3332091/148‑1 (MQ=255)
          cATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAg                                                                                                                                      <  2:2727293/148‑1 (MQ=255)
           aTTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGa                                                                                                                                     <  2:3436352/148‑1 (MQ=255)
            ttGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGAt                                                                                                                                    <  1:521823/148‑1 (MQ=255)
            ttGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGAt                                                                                                                                    <  1:1342154/148‑1 (MQ=255)
             tGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGAtt                                                                                                                                   >  1:2395927/1‑148 (MQ=255)
             tGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGAtt                                                                                                                                   <  2:895325/148‑1 (MQ=255)
              gAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTg                                                                                                                                  <  1:3113700/148‑1 (MQ=255)
                aCTCAATAACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGAGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTc                                                                                                                                >  2:1840735/1‑148 (MQ=255)
                 cTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCg                                                                                                                               >  1:683962/1‑148 (MQ=255)
                     aTCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCcgt                                                                                                                           >  2:107228/1‑148 (MQ=255)
                      tCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCcgtc                                                                                                                          <  2:1485593/148‑1 (MQ=255)
                      tCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCcgtc                                                                                                                          >  1:122699/1‑148 (MQ=255)
                       cACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCcgtcg                                                                                                                         <  2:3516403/148‑1 (MQ=255)
                       cACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCcgtcg                                                                                                                         <  2:2623617/148‑1 (MQ=255)
                       cACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCcgtcg                                                                                                                         <  2:617054/148‑1 (MQ=255)
                        aCTATCACGATCCGCGCGTTGCAGAACAGGCGGATATTGTGATTACTGGCGCGCAACCGCGGCGCAATAGCGTGACGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCcgtcgt                                                                                                                        >  2:539412/1‑148 (MQ=255)
                            tCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGaa                                                                                                                    >  2:1778853/1‑148 (MQ=255)
                             cACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGaaa                                                                                                                   >  1:2575215/1‑148 (MQ=255)
                             cACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGaaa                                                                                                                   >  1:938927/1‑148 (MQ=255)
                             cACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGaaa                                                                                                                   >  1:2575006/1‑148 (MQ=255)
                              aCGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAAc                                                                                                                  <  2:1928314/148‑1 (MQ=255)
                                  tCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAAc                                                                                                              >  2:1536918/1‑148 (MQ=255)
                                  tCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAAc                                                                                                              >  1:1138013/1‑148 (MQ=255)
                                  tCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAAc                                                                                                              <  1:431704/148‑1 (MQ=255)
                                   ccGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACt                                                                                                             >  1:788261/1‑148 (MQ=255)
                                   ccGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACt                                                                                                             <  2:824159/148‑1 (MQ=255)
                                       gcgGTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGcc                                                                                                         <  2:205133/148‑1 (MQ=255)
                                         gTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTCCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCg                                                                                                       <  1:2514330/148‑1 (MQ=255)
                                           tGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGAc                                                                                                     >  1:2815423/1‑148 (MQ=255)
                                           tGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGAc                                                                                                     >  2:339733/1‑148 (MQ=255)
                                            gCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACg                                                                                                    <  2:540203/148‑1 (MQ=255)
                                               gAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCg                                                                                                 <  1:2437210/148‑1 (MQ=255)
                                               gAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCg                                                                                                 <  1:650070/148‑1 (MQ=255)
                                                aaCTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCgg                                                                                                >  1:610866/1‑148 (MQ=255)
                                                 aCTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGt                                                                                               >  1:825097/1‑148 (MQ=255)
                                                 aCTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGt                                                                                               >  1:3014060/1‑148 (MQ=255)
                                                   tGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTaa                                                                                             <  2:2345189/148‑1 (MQ=255)
                                                    ggCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAat                                                                                            <  2:646119/148‑1 (MQ=255)
                                                     gCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAata                                                                                           >  2:2464243/1‑148 (MQ=255)
                                                       ggATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATg                                                                                         <  2:1112737/148‑1 (MQ=255)
                                                          tatTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTg                                                                                      >  1:328625/1‑148 (MQ=255)
                                                          tatTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTg                                                                                      >  2:3189023/1‑148 (MQ=255)
                                                             tgtgATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGAt                                                                                   >  1:3137012/1‑148 (MQ=255)
                                                               tgATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATaa                                                                                 <  2:2491263/148‑1 (MQ=255)
                                                               tgATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATaa                                                                                 <  2:2823308/148‑1 (MQ=255)
                                                                    ccTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCaaa                                                                            <  1:1097827/148‑1 (MQ=255)
                                                                    ccTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCaaa                                                                            <  1:952762/148‑1 (MQ=255)
                                                                    ccTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCaaa                                                                            >  1:1336763/1‑148 (MQ=255)
                                                                      tGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAAt                                                                          >  1:2352528/1‑148 (MQ=255)
                                                                         cgcgcCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATccc                                                                       <  1:3190670/148‑1 (MQ=255)
                                                                         cgcacCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGAAAAGCAAAATccc                                                                       <  2:2353569/148‑1 (MQ=255)
                                                                               aCCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGc                                                                 <  1:3475783/148‑1 (MQ=255)
                                                                                ccGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGcc                                                                <  2:909738/148‑1 (MQ=255)
                                                                                         aaTAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTg                                                       <  1:640136/148‑1 (MQ=255)
                                                                                             gCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGa                                                   <  2:689809/148‑1 (MQ=255)
                                                                                             gCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGa                                                   <  2:2162540/148‑1 (MQ=255)
                                                                                             gCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGa                                                   <  2:2162338/148‑1 (MQ=255)
                                                                                             gCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGa                                                   <  2:1572775/148‑1 (MQ=255)
                                                                                               gtgtCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATa                                                 <  1:2112369/148‑1 (MQ=255)
                                                                                                tgtCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATaa                                                <  1:1840735/148‑1 (MQ=255)
                                                                                                 gtCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAAc                                               >  1:1197926/1‑148 (MQ=255)
                                                                                                   cGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACg                                              <  1:2632966/147‑1 (MQ=255)
                                                                                                    gATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTg                                            <  2:2395927/148‑1 (MQ=255)
                                                                                                      tCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGt                                          >  2:1918231/1‑148 (MQ=255)
                                                                                                       cTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTc                                         >  1:1069602/1‑148 (MQ=255)
                                                                                                            aTGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGtt                                    <  2:2010834/148‑1 (MQ=255)
                                                                                                             tGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTc                                   >  1:711615/1‑148 (MQ=255)
                                                                                                              gCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCt                                  >  1:6357/1‑148 (MQ=255)
                                                                                                               cAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCtt                                 <  2:1341190/148‑1 (MQ=255)
                                                                                                                aaTGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTa                                <  2:1873341/148‑1 (MQ=255)
                                                                                                                 aTGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTAt                               >  2:2237552/1‑148 (MQ=255)
                                                                                                                 aTGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTAt                               >  1:2187529/1‑148 (MQ=255)
                                                                                                                  tGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTAtt                              >  2:70979/1‑148 (MQ=255)
                                                                                                                  tGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTAtt                              >  1:1177574/1‑148 (MQ=255)
                                                                                                                    gATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGc                            <  2:2957478/148‑1 (MQ=255)
                                                                                                                     aTCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCa                           >  2:1193674/1‑148 (MQ=255)
                                                                                                                     aTCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCa                           >  2:1193753/1‑148 (MQ=255)
                                                                                                                     aTCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCa                           >  2:2418219/1‑148 (MQ=255)
                                                                                                                      tCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAg                          >  2:3209102/1‑148 (MQ=255)
                                                                                                                       cgcgTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAgg                         >  2:189524/1‑148 (MQ=255)
                                                                                                                       cgcgTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAgg                         >  1:506779/1‑148 (MQ=255)
                                                                                                                        gcgTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGa                        <  2:1422480/148‑1 (MQ=255)
                                                                                                                            cGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATg                    <  2:2357005/148‑1 (MQ=255)
                                                                                                                               tACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGgcg                 <  2:3240580/148‑1 (MQ=255)
                                                                                                                               tACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGgcg                 <  1:345294/148‑1 (MQ=255)
                                                                                                                                aCCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGgcgc                >  2:2522196/1‑148 (MQ=255)
                                                                                                                                 cccGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCa               >  1:2378925/1‑148 (MQ=255)
                                                                                                                                 cccGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCa               <  1:1568234/148‑1 (MQ=255)
                                                                                                                                       aTGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCg         >  2:493748/1‑148 (MQ=255)
                                                                                                                                        tgttCAAATTGATCCGAAAAAGATTGTCGCCGTAGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTTGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGt        <  2:1028076/148‑1 (MQ=255)
                                                                                                                                        tgtgCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGt        <  2:2148144/148‑1 (MQ=255)
                                                                                                                                         gtgCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTa       <  1:1667778/148‑1 (MQ=255)
                                                                                                                                         gtgCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTa       >  2:995063/1‑148 (MQ=255)
                                                                                                                                              aaTTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTccg  >  1:1756010/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
AGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCG  >  CP009273/3058624‑3058913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCG  >  CP009273/3058621‑3058916
                                                                                                                                                    |                                                                                                                                                   
CGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTG                                                                                                                                                    >  SRR19973067.2360535/1‑150 (MQ=60)
CGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTG                                                                                                                                                    >  SRR19973067.2360525/1‑150 (MQ=60)
CGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTG                                                                                                                                                    >  SRR19973067.2360519/1‑150 (MQ=60)
 GAAGAAAGTGATCATTGAACTCACTCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGA                                                                                                                                                   <  SRR19973067.3547411/150‑1 (MQ=60)
 GAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGA                                                                                                                                                   <  SRR19973067.3281596/150‑1 (MQ=60)
 GAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGA                                                                                                                                                   <  SRR19973067.3281617/150‑1 (MQ=60)
 GAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGA                                                                                                                                                   >  SRR19973067.2383802/1‑150 (MQ=60)
     AAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCG                                                                                                                                               >  SRR19973067.360354/1‑150 (MQ=60)
     AAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCG                                                                                                                                               >  SRR19973067.2164056/1‑150 (MQ=60)
       AGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAA                                                                                                                                             >  SRR19973067.2045070/1‑150 (MQ=60)
       AGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAA                                                                                                                                             >  SRR19973067.2045090/1‑150 (MQ=60)
       AGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAA                                                                                                                                             >  SRR19973067.2045156/1‑150 (MQ=60)
            TCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGA                                                                                                                                        <  SRR19973067.3353175/150‑1 (MQ=60)
              ATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATT                                                                                                                                      <  SRR19973067.741613/150‑1 (MQ=60)
              ATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATT                                                                                                                                      <  SRR19973067.1214546/150‑1 (MQ=60)
              ATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATT                                                                                                                                      >  SRR19973067.2411536/1‑150 (MQ=60)
              ATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATT                                                                                                                                      >  SRR19973067.2411532/1‑150 (MQ=60)
               TTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTG                                                                                                                                     <  SRR19973067.1351331/150‑1 (MQ=60)
               TTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTG                                                                                                                                     <  SRR19973067.525347/150‑1 (MQ=60)
                TGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGT                                                                                                                                    >  SRR19973067.3427875/1‑150 (MQ=60)
                 GAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTC                                                                                                                                   <  SRR19973067.3133491/150‑1 (MQ=60)
                  AACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCG                                                                                                                                  >  SRR19973067.1350363/1‑150 (MQ=60)
                  AACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCG                                                                                                                                  >  SRR19973067.1566995/1‑150 (MQ=60)
                  AACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCG                                                                                                                                  >  SRR19973067.688657/1‑150 (MQ=60)
                  AACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCG                                                                                                                                  >  SRR19973067.688630/1‑150 (MQ=60)
                    CTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTTCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCC                                                                                                                                <  SRR19973067.1451338/150‑1 (MQ=60)
                    CTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCC                                                                                                                                >  SRR19973067.3296981/1‑150 (MQ=60)
                       AATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTC                                                                                                                             >  SRR19973067.123846/1‑150 (MQ=60)
                              ATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAA                                                                                                                      >  SRR19973067.2591796/1‑150 (MQ=60)
                              ATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAA                                                                                                                      >  SRR19973067.945313/1‑150 (MQ=60)
                              ATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAA                                                                                                                      >  SRR19973067.2591826/1‑150 (MQ=60)
                              ATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAA                                                                                                                      >  SRR19973067.2591926/1‑150 (MQ=60)
                              ATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAA                                                                                                                      >  SRR19973067.2592009/1‑150 (MQ=60)
                                   GATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAAC                                                                                                                 >  SRR19973067.1145703/1‑150 (MQ=60)
                                   GATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAAC                                                                                                                 >  SRR19973067.1145670/1‑150 (MQ=60)
                                    ATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACT                                                                                                                <  SRR19973067.685207/150‑1 (MQ=60)
                                    ATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACT                                                                                                                >  SRR19973067.2313336/1‑150 (MQ=60)
                                    ATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACT                                                                                                                >  SRR19973067.793708/1‑150 (MQ=60)
                                    ATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACT                                                                                                                >  SRR19973067.794646/1‑150 (MQ=60)
                                    ATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACT                                                                                                                >  SRR19973067.871601/1‑150 (MQ=60)
                                     TCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTT                                                                                                               <  SRR19973067.434702/150‑1 (MQ=60)
                                      CCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTG                                                                                                              >  SRR19973067.914352/1‑150 (MQ=60)
                                            GTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGAC                                                                                                        >  SRR19973067.2833556/1‑150 (MQ=60)
                                            GTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGAC                                                                                                        <  SRR19973067.2530790/150‑1 (MQ=60)
                                            GTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGAC                                                                                                        >  SRR19973067.2833667/1‑150 (MQ=60)
                                            GTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTCCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGAC                                                                                                        <  SRR19973067.2530817/150‑1 (MQ=60)
                                                 AGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGG                                                                                                   <  SRR19973067.478905/150‑1 (MQ=60)
                                                 AGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGG                                                                                                   >  SRR19973067.614822/1‑150 (MQ=60)
                                                 AGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGG                                                                                                   >  SRR19973067.649753/1‑150 (MQ=60)
                                                 AGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGG                                                                                                   <  SRR19973067.1345438/150‑1 (MQ=60)
                                                  GAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGT                                                                                                  <  SRR19973067.2453218/150‑1 (MQ=60)
                                                  GAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGT                                                                                                  <  SRR19973067.654479/150‑1 (MQ=60)
                                                  GAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGT                                                                                                  >  SRR19973067.3033269/1‑150 (MQ=60)
                                                  GAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGT                                                                                                  >  SRR19973067.3033238/1‑150 (MQ=60)
                                                  GAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGT                                                                                                  >  SRR19973067.830732/1‑150 (MQ=60)
                                                           GATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTG                                                                                         >  SRR19973067.330868/1‑150 (MQ=60)
                                                           GATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTG                                                                                         <  SRR19973067.519162/150‑1 (MQ=60)
                                                              ATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGAT                                                                                      >  SRR19973067.3156932/1‑150 (MQ=60)
                                                                    ATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAA                                                                                >  SRR19973067.1746670/1‑150 (MQ=60)
                                                                     TTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAA                                                                               >  SRR19973067.1345909/1‑150 (MQ=60)
                                                                       CCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAAT                                                                             <  SRR19973067.959286/150‑1 (MQ=60)
                                                                       CCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAAT                                                                             <  SRR19973067.958947/150‑1 (MQ=60)
                                                                       CCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAAT                                                                             >  SRR19973067.2367882/1‑150 (MQ=60)
                                                                       CCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAAT                                                                             <  SRR19973067.1105304/150‑1 (MQ=60)
                                                                         TGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCC                                                                           >  SRR19973067.318288/1‑150 (MQ=60)
                                                                            CGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCAT                                                                        <  SRR19973067.2056926/150‑1 (MQ=60)
                                                                            CGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCAT                                                                        <  SRR19973067.3210825/150‑1 (MQ=60)
                                                                               GCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTG                                                                     <  SRR19973067.2004951/150‑1 (MQ=60)
                                                                               GCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTG                                                                     <  SRR19973067.2563449/150‑1 (MQ=60)
                                                                               GCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTG                                                                     <  SRR19973067.2390387/150‑1 (MQ=60)
                                                                                  ACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCA                                                                  <  SRR19973067.3497855/150‑1 (MQ=60)
                                                                                        GCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGAT                                                            <  SRR19973067.3501228/150‑1 (MQ=60)
                                                                                          GCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTG                                                          <  SRR19973067.2377929/150‑1 (MQ=60)
                                                                                            AATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCC                                                        >  SRR19973067.3290453/1‑150 (MQ=60)
                                                                                            AATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCC                                                        <  SRR19973067.644443/150‑1 (MQ=60)
                                                                                            AATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCC                                                        <  SRR19973067.644048/150‑1 (MQ=60)
                                                                                             ATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCG                                                       <  SRR19973067.694841/150‑1 (MQ=60)
                                                                                                  GTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAAC                                                  <  SRR19973067.2126426/150‑1 (MQ=60)
                                                                                                  GTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAAC                                                  <  SRR19973067.1591550/150‑1 (MQ=60)
                                                                                                  GTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAAC                                                  >  SRR19973067.1205766/1‑150 (MQ=60)
                                                                                                  GTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAAC                                                  >  SRR19973067.1205772/1‑150 (MQ=60)
                                                                                                  GTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAAC                                                  <  SRR19973067.2126593/150‑1 (MQ=60)
                                                                                                   TGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACG                                                 <  SRR19973067.1853293/150‑1 (MQ=60)
                                                                                                   TGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACG                                                 >  SRR19973067.3133753/1‑150 (MQ=60)
                                                                                                     TCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTG                                               <  SRR19973067.2650005/150‑1 (MQ=60)
                                                                                                      CGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGG                                              >  SRR19973067.270318/1‑150 (MQ=60)
                                                                                                        ATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTC                                            >  SRR19973067.1077338/1‑150 (MQ=60)
                                                                                                        ATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTC                                            >  SRR19973067.1076935/1‑150 (MQ=60)
                                                                                                              CATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTC                                      >  SRR19973067.716543/1‑150 (MQ=60)
                                                                                                              CATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTC                                      >  SRR19973067.2622937/1‑150 (MQ=60)
                                                                                                               ATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCATCTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCT                                     >  SRR19973067.289085/1‑150 (MQ=60)
                                                                                                               ATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCT                                     >  SRR19973067.6451/1‑150 (MQ=60)
                                                                                                                TGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTT                                    <  SRR19973067.624162/150‑1 (MQ=60)
                                                                                                                TGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTT                                    <  SRR19973067.624581/150‑1 (MQ=60)
                                                                                                                TGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTT                                    <  SRR19973067.1185695/150‑1 (MQ=60)
                                                                                                                 GCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTA                                   <  SRR19973067.1798751/150‑1 (MQ=60)
                                                                                                                  CAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTAT                                  >  SRR19973067.2202139/1‑150 (MQ=60)
                                                                                                                   AATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATT                                 <  SRR19973067.1959244/150‑1 (MQ=60)
                                                                                                                   AATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATT                                 >  SRR19973067.1185359/1‑150 (MQ=60)
                                                                                                                        ATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGG                            >  SRR19973067.510243/1‑150 (MQ=60)
                                                                                                                               CGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGC                     >  SRR19973067.552018/1‑150 (MQ=60)
                                                                                                                                  TACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCA                  >  SRR19973067.2394457/1‑150 (MQ=60)
                                                                                                                                  TACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCA                  >  SRR19973067.2394381/1‑150 (MQ=60)
                                                                                                                                  TACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCA                  >  SRR19973067.2394379/1‑150 (MQ=60)
                                                                                                                                  TACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCA                  <  SRR19973067.347678/150‑1 (MQ=60)
                                                                                                                                  TACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCA                  <  SRR19973067.347824/150‑1 (MQ=60)
                                                                                                                                    CCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATG                <  SRR19973067.1578960/150‑1 (MQ=60)
                                                                                                                                     CCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGG               <  SRR19973067.3318119/150‑1 (MQ=60)
                                                                                                                                            GTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATT        <  SRR19973067.1679283/150‑1 (MQ=60)
                                                                                                                                             TGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTC       >  SRR19973067.1124686/1‑150 (MQ=60)
                                                                                                                                               CAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCG     >  SRR19973067.1767971/1‑150 (MQ=60)
                                                                                                                                                  ATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCG  >  SRR19973067.20868/1‑150 (MQ=60)
                                                                                                                                                  ATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCAGCCG  >  SRR19973067.1833234/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                   
CGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCG  >  CP009273/3058621‑3058916

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑