Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,058,769 C→T 100% I203I (ATC→ATT)  scpC → propionyl‑CoA:succinate CoA transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,058,7690CT99.2% 780.4 / ‑2.3 239I203I (ATC→ATT) scpCpropionyl‑CoA:succinate CoA transferase
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (118/119);  total (119/120)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 239 total aligned reads displayed.

GAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCG  >  CP009273/3058625‑3058913
                                                                                                                                                |                                                                                                                                                
gaaAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGAt                                                                                                                                               <  2:1082164/148‑1 (MQ=255)
 aaaGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATc                                                                                                                                              <  1:368563/148‑1 (MQ=255)
 aaaGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATc                                                                                                                                              >  2:2163702/1‑148 (MQ=255)
   aGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCg                                                                                                                                            >  1:870533/1‑148 (MQ=255)
       aTCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGaaaa                                                                                                                                        >  1:2540060/1‑148 (MQ=255)
       aTCATTGAACTCAATCACTATCACGATCCGCGAGTTTCAGAACTGGCGGATATTGTGATTCCTGGCGCGCAACCTCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGATATGTGCAAATTGATCCGaaaa                                                                                                                                        <  2:546744/148‑1 (MQ=255)
         cATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAg                                                                                                                                      <  1:1863272/148‑1 (MQ=255)
            tGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGAtt                                                                                                                                   <  1:284576/148‑1 (MQ=255)
            tGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGAtt                                                                                                                                   >  2:3066768/1‑148 (MQ=255)
            tGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGAtt                                                                                                                                   <  1:2002174/148‑1 (MQ=255)
             gAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTg                                                                                                                                  <  1:3202576/148‑1 (MQ=255)
             gAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTg                                                                                                                                  <  1:2456317/148‑1 (MQ=255)
             gAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTg                                                                                                                                  <  1:1005574/148‑1 (MQ=255)
                 tCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCAGAAAAAGATTGTCGc                                                                                                                              >  2:93700/1‑148 (MQ=255)
                  cAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGcc                                                                                                                             >  2:1485299/1‑148 (MQ=255)
                   aaTCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCcg                                                                                                                            <  2:345181/148‑1 (MQ=255)
                    aTCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCcgt                                                                                                                           >  2:1156806/1‑148 (MQ=255)
                     tCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCcgtc                                                                                                                          <  2:412321/148‑1 (MQ=255)
                       aCTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCcgtcgt                                                                                                                        >  2:1265286/1‑148 (MQ=255)
                        cTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTg                                                                                                                       >  1:23808/1‑148 (MQ=255)
                        cTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTg                                                                                                                       >  1:1801421/1‑148 (MQ=255)
                         tATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTgg                                                                                                                      <  1:132202/148‑1 (MQ=255)
                            cACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGAGTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGaaa                                                                                                                   <  1:3343395/148‑1 (MQ=255)
                              cGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCCCCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAAcc                                                                                                                 >  1:430689/1‑148 (MQ=255)
                              cGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAAcc                                                                                                                 >  1:2829225/1‑148 (MQ=255)
                               gATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCa                                                                                                                <  1:1485299/148‑1 (MQ=255)
                                aTCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCaa                                                                                                               <  1:3492998/148‑1 (MQ=255)
                                  ccGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACt                                                                                                             <  2:2176201/148‑1 (MQ=255)
                                   cgcgcgTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACtt                                                                                                            >  1:635355/1‑148 (MQ=255)
                                    gcgcgTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTg                                                                                                           <  1:2048321/148‑1 (MQ=255)
                                     cgcgTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGc                                                                                                          >  1:804141/1‑148 (MQ=255)
                                      gcgTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGcc                                                                                                         <  1:257529/148‑1 (MQ=255)
                                       cgTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGtcc                                                                                                        >  1:1243590/1‑148 (MQ=255)
                                             aGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGaa                                                                                                                    <  1:2401425/130‑1 (MQ=255)
                                               aaCTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCgg                                                                                                <  1:2761839/148‑1 (MQ=255)
                                                aCTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGt                                                                                               <  2:1636748/148‑1 (MQ=255)
                                                aCTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGt                                                                                               >  2:1356139/1‑148 (MQ=255)
                                                   ggCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAat                                                                                            >  2:798983/1‑148 (MQ=255)
                                                    gCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAata                                                                                           >  1:2914244/1‑148 (MQ=255)
                                                       gATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGc                                                                                        >  1:2601356/1‑148 (MQ=255)
                                                        atatTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTAGATCTACCATGCAATCGATCGCGTCGGTACCCGCAATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGACACCAACTTGCCCGACGCCGGTAATATGCt                                                                                       >  2:3309119/1‑148 (MQ=255)
                                                          atTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTgg                                                                                     <  2:2515234/148‑1 (MQ=255)
                                                          atTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTgg                                                                                     >  1:122360/1‑148 (MQ=255)
                                                          atTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTgg                                                                                     >  1:121958/1‑148 (MQ=255)
                                                            tgtgATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGAt                                                                                   >  1:1101624/1‑148 (MQ=255)
                                                            tgtgATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGAt                                                                                   >  1:344916/1‑148 (MQ=255)
                                                             gtgATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATa                                                                                  <  1:2622311/148‑1 (MQ=255)
                                                             gtgATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATa                                                                                  <  1:2621492/148‑1 (MQ=255)
                                                              tgATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATaa                                                                                 <  2:1890794/148‑1 (MQ=255)
                                                                aTTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGc                                                                               >  2:536887/1‑148 (MQ=255)
                                                                aTTCCTGGAGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATTCAATTGATCGCGTCGGTACCCGCTATTTGCAAATTGATCCGAAAAAGATTGTCGCCTTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGc                                                                               <  2:1392820/148‑1 (MQ=255)
                                                                  tCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCaa                                                                             >  2:2897559/1‑148 (MQ=255)
                                                                  tCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCaa                                                                             >  1:455123/1‑148 (MQ=255)
                                                                   ccTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCaaa                                                                            <  1:1153106/148‑1 (MQ=255)
                                                                   ccTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTGGCCCGACGCCGGTAATATGCTGGATAAGCaaa                                                                            <  2:2137120/148‑1 (MQ=255)
                                                                      ggCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATc                                                                         >  1:176991/1‑148 (MQ=255)
                                                                          cgcCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCAt                                                                     >  1:467065/1‑148 (MQ=255)
                                                                          cgcCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCAt                                                                     >  1:466996/1‑148 (MQ=255)
                                                                           gcCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCAtg                                                                    <  1:1240097/148‑1 (MQ=255)
                                                                              aCCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGAATAAGCAAAATCCCATGTGc                                                                 >  2:2839794/1‑148 (MQ=255)
                                                                               ccGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGcc                                                                >  1:1427657/1‑148 (MQ=255)
                                                                               ccGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGcc                                                                <  2:1545241/148‑1 (MQ=255)
                                                                                cgcgGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCca                                                               >  2:3461245/1‑148 (MQ=255)
                                                                                 gcggcgCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCcag                                                              <  2:1666345/148‑1 (MQ=255)
                                                                                      gcAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGAt                                                         <  1:474022/148‑1 (MQ=255)
                                                                                        aaTAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTg                                                       >  1:3341984/1‑148 (MQ=255)
                                                                                         aTAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGc                                                      <  2:102120/148‑1 (MQ=255)
                                                                                         aTAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGc                                                      >  1:2075630/1‑148 (MQ=255)
                                                                                          tAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGcc                                                     >  2:163754/1‑148 (MQ=255)
                                                                                          tAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGcc                                                     >  2:597552/1‑148 (MQ=255)
                                                                                           aGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCg                                                    <  1:2167156/148‑1 (MQ=255)
                                                                                           aGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCg                                                    <  1:666300/148‑1 (MQ=255)
                                                                                                 tCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACg                                              >  1:1684636/1‑148 (MQ=255)
                                                                                                  cGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGt                                             >  1:3497538/1‑148 (MQ=255)
                                                                                                   gATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTg                                            <  2:550229/148‑1 (MQ=255)
                                                                                                       ttCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCa                                        >  2:767904/1‑148 (MQ=255)
                                                                                                       ttCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCa                                        >  2:53860/1‑148 (MQ=255)
                                                                                                         ccATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACg                                      >  1:732579/1‑148 (MQ=255)
                                                                                                          cATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGt                                     >  2:3172262/1‑148 (MQ=255)
                                                                                                           aTGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGtt                                    <  2:2009125/148‑1 (MQ=255)
                                                                                                             gCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCt                                  <  2:3401056/148‑1 (MQ=255)
                                                                                                               aaTGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTa                                <  2:756792/148‑1 (MQ=255)
                                                                                                               aaTGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTa                                <  2:2517724/148‑1 (MQ=255)
                                                                                                                    aTCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCa                           <  1:2152130/148‑1 (MQ=255)
                                                                                                                    aTCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCa                           >  2:1771827/1‑148 (MQ=255)
                                                                                                                     tCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAg                          >  2:1159580/1‑148 (MQ=255)
                                                                                                                      cgcgTCGGTACCCGCTATGTGCAAATTGATCAGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTTCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATAGCAGATAACGTGGTCACGTTCTTATTGCAgg                         >  2:1826185/1‑148 (MQ=255)
                                                                                                                        cgTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGaa                       <  2:1135121/148‑1 (MQ=255)
                                                                                                                         gTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGaaa                      <  2:875900/148‑1 (MQ=255)
                                                                                                                          tCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAAt                     <  2:2334435/148‑1 (MQ=255)
                                                                                                                          tCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAAt                     <  2:344916/148‑1 (MQ=255)
                                                                                                                                cccGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCa               <  2:2776031/148‑1 (MQ=255)
                                                                                                                                 ccGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTGGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCAt              <  2:1168700/148‑1 (MQ=255)
                                                                                                                                  cGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATg             <  2:2947046/148‑1 (MQ=255)
                                                                                                                                    cTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATggg           <  2:1695755/148‑1 (MQ=255)
                                                                                                                                       tgtgCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGt        >  1:3266693/1‑148 (MQ=255)
                                                                                                                                       tgtgCAAATTGATCCGAAAAAGAGTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGt        <  1:2998330/148‑1 (MQ=255)
                                                                                                                                         tgCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTAt      <  1:1631064/148‑1 (MQ=255)
                                                                                                                                          gCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTAtt     <  1:1069575/148‑1 (MQ=255)
                                                                                                                                             aaTTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGCACTGGCGCATGGGCGTCTTccg  >  2:499340/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
GAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCG  >  CP009273/3058625‑3058913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCG  >  CP009273/3058620‑3058916
                                                                                                                                                     |                                                                                                                                                   
GCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATT                                                                                                                                                     <  SRR19973065.1473550/150‑1 (MQ=60)
 CGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTG                                                                                                                                                    <  SRR19973065.1435244/150‑1 (MQ=60)
     GAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCCCCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCC                                                                                                                                                >  SRR19973065.2182574/1‑150 (MQ=60)
      AAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCG                                                                                                                                               <  SRR19973065.371146/150‑1 (MQ=60)
      AAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCG                                                                                                                                               <  SRR19973065.371165/150‑1 (MQ=60)
      AAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCG                                                                                                                                               >  SRR19973065.535730/1‑150 (MQ=60)
      AAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCG                                                                                                                                               >  SRR19973065.876109/1‑150 (MQ=60)
      AAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCG                                                                                                                                               >  SRR19973065.955152/1‑150 (MQ=60)
       AAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGA                                                                                                                                              >  SRR19973065.1143161/1‑150 (MQ=60)
       AAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGA                                                                                                                                              >  SRR19973065.1142479/1‑150 (MQ=60)
          TGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAA                                                                                                                                           >  SRR19973065.2555979/1‑150 (MQ=60)
          TGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAA                                                                                                                                           >  SRR19973065.2557036/1‑150 (MQ=60)
            ATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAG                                                                                                                                         >  SRR19973065.2637829/1‑150 (MQ=60)
            ATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAG                                                                                                                                         <  SRR19973065.13017/150‑1 (MQ=60)
             TCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGA                                                                                                                                        <  SRR19973065.2896349/150‑1 (MQ=60)
              CATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGAT                                                                                                                                       <  SRR19973065.1875535/150‑1 (MQ=60)
                 TGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGT                                                                                                                                    <  SRR19973065.2015127/150‑1 (MQ=60)
                 TGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGT                                                                                                                                    <  SRR19973065.286451/150‑1 (MQ=60)
                  GAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTC                                                                                                                                   <  SRR19973065.3221857/150‑1 (MQ=60)
                  GAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTC                                                                                                                                   <  SRR19973065.1012251/150‑1 (MQ=60)
                  GAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTC                                                                                                                                   <  SRR19973065.2471796/150‑1 (MQ=60)
                        AATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTC                                                                                                                             >  SRR19973065.2957550/1‑150 (MQ=60)
                         ATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCG                                                                                                                            >  SRR19973065.2547545/1‑150 (MQ=60)
                         ATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCG                                                                                                                            >  SRR19973065.2547640/1‑150 (MQ=60)
                         ATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCG                                                                                                                            >  SRR19973065.2793261/1‑150 (MQ=60)
                           CACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTG                                                                                                                          >  SRR19973065.849750/1‑150 (MQ=60)
                           CACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTG                                                                                                                          >  SRR19973065.24127/1‑150 (MQ=60)
                           CACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTG                                                                                                                          >  SRR19973065.1813250/1‑150 (MQ=60)
                              TATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAA                                                                                                                       <  SRR19973065.133338/150‑1 (MQ=60)
                               ATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAA                                                                                                                      >  SRR19973065.996967/1‑150 (MQ=60)
                                tgtgctcttccgatcTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAAC                                                                                                                     <  SRR19973065.2416523/135‑1 (MQ=60)
                                 CACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCCCCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACC                                                                                                                    >  SRR19973065.433682/1‑150 (MQ=60)
                                 CACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACC                                                                                                                    >  SRR19973065.2846687/1‑150 (MQ=60)
                                 CACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACC                                                                                                                    <  SRR19973065.1615453/150‑1 (MQ=60)
                                 CACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACC                                                                                                                    >  SRR19973065.2846681/1‑150 (MQ=60)
                                 CACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACC                                                                                                                    <  SRR19973065.3363815/150‑1 (MQ=60)
                                 CACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGAGTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACC                                                                                                                    <  SRR19973065.3363821/150‑1 (MQ=60)
                                 CACGATCAGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCTCGCCAACGCGGCGCAATAGCTTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACC                                                                                                                    >  SRR19973065.2846744/1‑150 (MQ=60)
                                    GATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAAC                                                                                                                 >  SRR19973065.3083605/1‑150 (MQ=60)
                                    GATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAAC                                                                                                                 <  SRR19973065.1494773/150‑1 (MQ=60)
                                     ATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACT                                                                                                                <  SRR19973065.3514302/150‑1 (MQ=60)
                                     ATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACT                                                                                                                <  SRR19973065.3514234/150‑1 (MQ=60)
                                      TCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTT                                                                                                               >  SRR19973065.639505/1‑150 (MQ=60)
                                        CGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGC                                                                                                             >  SRR19973065.809457/1‑150 (MQ=60)
                                         GCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCC                                                                                                            <  SRR19973065.2061413/150‑1 (MQ=60)
                                          CGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGTCC                                                                                                           >  SRR19973065.1251769/1‑150 (MQ=60)
                                          CGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGTCC                                                                                                           >  SRR19973065.1251182/1‑150 (MQ=60)
                                          CGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCC                                                                                                           <  SRR19973065.1552340/150‑1 (MQ=60)
                                           GCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCG                                                                                                          <  SRR19973065.2647334/150‑1 (MQ=60)
                                           GCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCG                                                                                                          <  SRR19973065.317069/150‑1 (MQ=60)
                                           GCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCG                                                                                                          <  SRR19973065.259317/150‑1 (MQ=60)
                                                GCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCC                                                                                                     <  SRR19973065.1273624/150‑1 (MQ=60)
                                                   GAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGT                                                                                                  >  SRR19973065.2634419/1‑150 (MQ=60)
                                                    AACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTA                                                                                                 <  SRR19973065.2778988/150‑1 (MQ=60)
                                                    AACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTA                                                                                                 <  SRR19973065.2779001/150‑1 (MQ=60)
                                                       TGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATA                                                                                              >  SRR19973065.2931972/1‑150 (MQ=60)
                                                        GGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATAT                                                                                             <  SRR19973065.1239471/150‑1 (MQ=60)
                                                          CGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGC                                                                                           >  SRR19973065.2617512/1‑150 (MQ=60)
                                                            GATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTG                                                                                         >  SRR19973065.43774/1‑150 (MQ=60)
                                                            GATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTG                                                                                         <  SRR19973065.3506439/150‑1 (MQ=60)
                                                             ATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGG                                                                                        >  SRR19973065.123023/1‑150 (MQ=60)
                                                             ATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGG                                                                                        >  SRR19973065.123440/1‑150 (MQ=60)
                                                             ATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGG                                                                                        >  SRR19973065.123427/1‑150 (MQ=60)
                                                             ATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGG                                                                                        >  SRR19973065.123028/1‑150 (MQ=60)
                                                               ATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGAT                                                                                      >  SRR19973065.347329/1‑150 (MQ=60)
                                                               ATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGAT                                                                                      >  SRR19973065.1108861/1‑150 (MQ=60)
                                                                  GTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAG                                                                                   <  SRR19973065.2637719/150‑1 (MQ=60)
                                                                  GTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAG                                                                                   <  SRR19973065.2638541/150‑1 (MQ=60)
                                                                     ATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAA                                                                                >  SRR19973065.1369306/1‑150 (MQ=60)
                                                                     ATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAA                                                                                >  SRR19973065.458271/1‑150 (MQ=60)
                                                                     ATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAA                                                                                >  SRR19973065.458327/1‑150 (MQ=60)
                                                                        CCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAAT                                                                             <  SRR19973065.3241457/150‑1 (MQ=60)
                                                                        CCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAAT                                                                             <  SRR19973065.1160520/150‑1 (MQ=60)
                                                                        CCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAAT                                                                             <  SRR19973065.1160531/150‑1 (MQ=60)
                                                                         CTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATC                                                                            >  SRR19973065.178393/1‑150 (MQ=60)
                                                                          TGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCC                                                                           >  SRR19973065.3432383/1‑150 (MQ=60)
                                                                             CGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCAT                                                                        >  SRR19973065.470210/1‑150 (MQ=60)
                                                                             CGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCAT                                                                        >  SRR19973065.470276/1‑150 (MQ=60)
                                                                             CGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCAT                                                                        <  SRR19973065.3524906/150‑1 (MQ=60)
                                                                             CGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCAT                                                                        >  SRR19973065.470280/1‑150 (MQ=60)
                                                                               CGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGT                                                                      <  SRR19973065.2082586/150‑1 (MQ=60)
                                                                                GCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTG                                                                     <  SRR19973065.1248245/150‑1 (MQ=60)
                                                                                  CACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCC                                                                   >  SRR19973065.1436953/1‑150 (MQ=60)
                                                                                  CACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCC                                                                   >  SRR19973065.1707072/1‑150 (MQ=60)
                                                                                      GCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCACATGTGCCAGCA                                                               <  SRR19973065.464669/150‑1 (MQ=60)
                                                                                        GGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGA                                                             >  SRR19973065.1983563/1‑150 (MQ=60)
                                                                                          CGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATT                                                           <  SRR19973065.539596/150‑1 (MQ=60)
                                                                                          CGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATT                                                           <  SRR19973065.1950346/150‑1 (MQ=60)
                                                                                          CGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATT                                                           <  SRR19973065.1734397/150‑1 (MQ=60)
                                                                                          CGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATT                                                           <  SRR19973065.3480231/150‑1 (MQ=60)
                                                                                           GCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTG                                                          <  SRR19973065.477260/150‑1 (MQ=60)
                                                                                           GCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTG                                                          >  SRR19973065.3362360/1‑150 (MQ=60)
                                                                                           GCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTG                                                          >  SRR19973065.3362403/1‑150 (MQ=60)
                                                                                            CAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGC                                                         >  SRR19973065.2088896/1‑150 (MQ=60)
                                                                                            CAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGC                                                         >  SRR19973065.2088897/1‑150 (MQ=60)
                                                                                                AGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGAT                                                     <  SRR19973065.670735/150‑1 (MQ=60)
                                                                                                AGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGAT                                                     <  SRR19973065.2454672/150‑1 (MQ=60)
                                                                                                AGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGAT                                                     <  SRR19973065.2181031/150‑1 (MQ=60)
                                                                                                AGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGAT                                                     <  SRR19973065.2181079/150‑1 (MQ=60)
                                                                                                  CGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAA                                                   >  SRR19973065.405872/1‑150 (MQ=60)
                                                                                                    TGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACG                                                 >  SRR19973065.1695865/1‑150 (MQ=60)
                                                                                                    TGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACG                                                 >  SRR19973065.1695898/1‑150 (MQ=60)
                                                                                                    TGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACG                                                 <  SRR19973065.475613/150‑1 (MQ=60)
                                                                                                     GTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGT                                                >  SRR19973065.3518789/1‑150 (MQ=60)
                                                                                                        GATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGT                                             >  SRR19973065.652826/1‑150 (MQ=60)
                                                                                                        GATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGT                                             >  SRR19973065.2611274/1‑150 (MQ=60)
                                                                                                        GATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGT                                             <  SRR19973065.2288136/150‑1 (MQ=60)
                                                                                                          TCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAANCCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCA                                           <  SRR19973065.1791662/150‑1 (MQ=60)
                                                                                                           CTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCAC                                          >  SRR19973065.2042847/1‑150 (MQ=60)
                                                                                                            TTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACG                                         >  SRR19973065.737480/1‑150 (MQ=60)
                                                                                                                   CAATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAATAGATTGTCGACGTCGTGGAAACCAACGTGCCCGACGCCGGTAATATGATGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTAT                                  <  SRR19973065.3329286/150‑1 (MQ=60)
                                                                                                                     ATGGATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTG                                >  SRR19973065.1090472/1‑150 (MQ=60)
                                                                                                                         ATCGCGTCGGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGG                            <  SRR19973065.2165968/150‑1 (MQ=60)
                                                                                                                                 GGTACCCGCTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCG                    <  SRR19973065.1058508/150‑1 (MQ=60)
                                                                                                                                         CTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCG            <  SRR19973065.704762/150‑1 (MQ=60)
                                                                                                                                         CTATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCG            <  SRR19973065.701125/150‑1 (MQ=60)
                                                                                                                                          TATGTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGT           >  SRR19973065.3286499/1‑150 (MQ=60)
                                                                                                                                            TGTGCAAATTGATCCGAAAAAGAGTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTAT         <  SRR19973065.3016696/150‑1 (MQ=60)
                                                                                                                                             GTGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATT        <  SRR19973065.2145042/150‑1 (MQ=60)
                                                                                                                                              TGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTC       <  SRR19973065.1642013/150‑1 (MQ=60)
                                                                                                                                              TGCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTC       >  SRR19973065.2606729/1‑150 (MQ=60)
                                                                                                                                               GCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCC      <  SRR19973065.1832969/150‑1 (MQ=60)
                                                                                                                                               GCAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCC      <  SRR19973065.1076656/150‑1 (MQ=60)
                                                                                                                                                CAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCG     >  SRR19973065.2407350/1‑150 (MQ=60)
                                                                                                                                                CAAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCG     <  SRR19973065.1481633/150‑1 (MQ=60)
                                                                                                                                                 AAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGC    >  SRR19973065.2039511/1‑150 (MQ=60)
                                                                                                                                                 AAATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGC    >  SRR19973065.2039136/1‑150 (MQ=60)
                                                                                                                                                  AATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCC   >  SRR19973065.647964/1‑150 (MQ=60)
                                                                                                                                                  AATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCC   <  SRR19973065.75096/150‑1 (MQ=60)
                                                                                                                                                   ATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCG  >  SRR19973065.2975937/1‑150 (MQ=60)
                                                                                                                                                   ATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCG  >  SRR19973065.2975945/1‑150 (MQ=60)
                                                                                                                                                   ATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCG  >  SRR19973065.1517651/1‑150 (MQ=60)
                                                                                                                                                   ATTGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCG  >  SRR19973065.3082543/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                   
GCGAAGAAAGTGATCATTGAACTCAATCACTATCACGATCCGCGCGTTGCAGAACTGGCGGATATTGTGATTCCTGGCGCGCCACCGCGGCGCAATAGCGTGTCGATCTTCCATGCAATGGATCGCGTCGGTACCCGCTATGTGCAAATCGATCCGAAAAAGATTGTCGCCGTCGTGGAAACCAACTTGCCCGACGCCGGTAATATGCTGGATAAGCAAAATCCCATGTGCCAGCAGATTGCCGATAACGTGGTCACGTTCTTATTGCAGGAAATGGCGCATGGGCGTATTCCGCCG  >  CP009273/3058620‑3058916

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑