Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,201,209 C→A 100% F160L (TTC→TTA)  ttdB → L‑tartrate dehydratase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,201,2090CA97.6% 500.9 / 4.0 167F160L (TTC→TTA) ttdBL‑tartrate dehydratase, beta subunit
Reads supporting (aligned to +/- strand):  ref base C (4/0);  new base A (91/71);  total (95/72)
Fisher's exact test for biased strand distribution p-value = 1.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 167 total aligned reads displayed.

TGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTCAC  >  CP009273/3201066‑3201354
                                                                                                                                               |                                                                                                                                                 
tGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGcc                                                                                                                                               <  1:625613/148‑1 (MQ=255)
  ccAGAAATTCACGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCg                                                                                                                                             <  2:2135783/148‑1 (MQ=255)
        aTTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGAtt                                                                                                                                       <  2:2994465/148‑1 (MQ=255)
        aTTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGAtt                                                                                                                                       >  1:1770944/1‑148 (MQ=255)
          tCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGt                                                                                                                                     <  1:3166752/148‑1 (MQ=255)
          tCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGt                                                                                                                                     >  2:3337944/1‑148 (MQ=255)
            aaGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGtct                                                                                                                                   <  1:509446/148‑1 (MQ=255)
              ggCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTctct                                                                                                                                 <  2:1944239/148‑1 (MQ=255)
              ggCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTctct                                                                                                                                 <  2:1943613/148‑1 (MQ=255)
                cgcTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTAt                                                                                                                               >  2:587453/1‑148 (MQ=255)
                 gcTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTAtt                                                                                                                              <  2:3022451/148‑1 (MQ=255)
                   tACATGTGATGTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGa                                                                                                                            <  1:2419729/148‑1 (MQ=255)
                    aCATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGAt                                                                                                                           >  2:601553/1‑148 (MQ=255)
                     cATGTGATTTTCCCGGCTGGCTGCGCGGTTCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATa                                                                                                                          <  1:2717679/148‑1 (MQ=255)
                      aTGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATAc                                                                                                                         >  2:2071252/1‑148 (MQ=255)
                       tgtgATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATAcc                                                                                                                        <  1:1272785/148‑1 (MQ=255)
                       tgtgATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATAcc                                                                                                                        >  1:3086279/1‑148 (MQ=255)
                       tgtgATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATAcc                                                                                                                        >  1:3011543/1‑148 (MQ=255)
                        gtgATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATAccc                                                                                                                       >  2:817516/1‑148 (MQ=255)
                         tgATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCa                                                                                                                      >  1:1003733/1‑148 (MQ=255)
                         tgATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCa                                                                                                                      >  1:2780257/1‑148 (MQ=255)
                             tttCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGc                                                                                                                  >  1:2387563/1‑148 (MQ=255)
                              ttCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGca                                                                                                                 <  2:949039/148‑1 (MQ=255)
                                cccGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGcaac                                                                                                               >  1:3434393/1‑148 (MQ=255)
                                cccGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGcaac                                                                                                               >  1:3434269/1‑148 (MQ=255)
                                    gCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAAcc                                                                                                           >  2:2580121/1‑148 (MQ=255)
                                    gCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAAcc                                                                                                           >  2:3027631/1‑148 (MQ=255)
                                    gCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAAcc                                                                                                           >  2:3027633/1‑148 (MQ=255)
                                       ggCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGa                                                                                                        >  2:292023/1‑148 (MQ=255)
                                        gCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGAt                                                                                                       >  2:909484/1‑148 (MQ=255)
                                        gCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGAt                                                                                                       >  1:1734035/1‑148 (MQ=255)
                                        gCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGAt                                                                                                       >  1:432242/1‑148 (MQ=255)
                                        gCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGAt                                                                                                       >  1:3359682/1‑148 (MQ=255)
                                              cgGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGa                                                                                                 <  2:2079522/148‑1 (MQ=255)
                                                 tGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGaaaa                                                                                              <  2:802081/148‑1 (MQ=255)
                                                   cTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACa                                                                                            <  2:3038818/148‑1 (MQ=255)
                                                     ggcggcAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGGTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACaaa                                                                                          >  1:1429160/1‑148 (MQ=255)
                                                      gcggcAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACaaaa                                                                                         <  2:1392512/148‑1 (MQ=255)
                                                      gcggcAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACaaaa                                                                                         <  2:1392335/148‑1 (MQ=255)
                                                          cAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCt                                                                                     >  2:2550863/1‑148 (MQ=255)
                                                          cAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCt                                                                                     <  2:1160495/148‑1 (MQ=255)
                                                            aCCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGt                                                                                   <  1:1512380/148‑1 (MQ=255)
                                                            aCCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGt                                                                                   <  1:1512463/148‑1 (MQ=255)
                                                            aCCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGt                                                                                   >  2:2554665/1‑148 (MQ=255)
                                                             cccAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGTCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGtt                                                                                  <  1:1155590/148‑1 (MQ=255)
                                                             cccAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGtt                                                                                  >  1:979294/1‑148 (MQ=255)
                                                               cAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCg                                                                                >  1:2173810/1‑148 (MQ=255)
                                                                 ggTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGcc                                                                              <  1:604253/148‑1 (MQ=255)
                                                                  gTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCg                                                                             <  2:515543/148‑1 (MQ=255)
                                                                   tGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGa                                                                            <  1:1221270/148‑1 (MQ=255)
                                                                    ggAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGaa                                                                           <  1:2019404/148‑1 (MQ=255)
                                                                     gAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAAc                                                                          >  2:2610684/1‑148 (MQ=255)
                                                                         agaTTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAAcgccg                                                                      <  1:88338/148‑1 (MQ=255)
                                                                         agaTTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAAcgccg                                                                      >  2:175662/1‑148 (MQ=255)
                                                                          gaTTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAAcgccgc                                                                     >  1:568013/1‑148 (MQ=255)
                                                                             tGAAGAAGTGCACGGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGAt                                                                  <  2:1613871/148‑1 (MQ=255)
                                                                                 gaagTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCa                                                              >  2:81316/1‑148 (MQ=255)
                                                                                         cTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGaa                                                      >  1:1891212/1‑148 (MQ=255)
                                                                                             aCAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAgaga                                                  <  1:76444/148‑1 (MQ=255)
                                                                                             aCAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAgaga                                                  <  1:962295/148‑1 (MQ=255)
                                                                                                gagCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCt                                               >  2:33691/1‑148 (MQ=255)
                                                                                                   cTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCg                                            >  1:1881761/1‑148 (MQ=255)
                                                                                                   cTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCg                                            <  1:417085/148‑1 (MQ=255)
                                                                                                     cGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAg                                          >  2:498918/1‑148 (MQ=255)
                                                                                                      ggtaTGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGc                                         <  2:2985652/145‑1 (MQ=255)
                                                                                                      ggAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGc                                         >  1:434933/1‑148 (MQ=255)
                                                                                                        aaTGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCAc                                       >  2:479278/1‑148 (MQ=255)
                                                                                                        aaTGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCAc                                       >  1:263116/1‑148 (MQ=255)
                                                                                                        aaTGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCAc                                       >  2:6539/1‑148 (MQ=255)
                                                                                                        aaTGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCAc                                       >  1:263167/1‑148 (MQ=255)
                                                                                                          tGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGt                                     >  1:3086842/1‑148 (MQ=255)
                                                                                                           gCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTc                                    <  2:1946992/148‑1 (MQ=255)
                                                                                                              ggAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCAt                                 <  1:2021002/148‑1 (MQ=255)
                                                                                                               gAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCAtt                                >  2:887167/1‑148 (MQ=255)
                                                                                                                aGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTa                               >  1:1066321/1‑148 (MQ=255)
                                                                                                                aGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTa                               >  2:3230574/1‑148 (MQ=255)
                                                                                                                aGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTa                               >  2:3229908/1‑148 (MQ=255)
                                                                                                                 gTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTAc                              <  1:173803/148‑1 (MQ=255)
                                                                                                                   cACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACAt                            <  2:3413920/148‑1 (MQ=255)
                                                                                                                     cTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCa                          >  2:2780528/1‑148 (MQ=255)
                                                                                                                      tgtgGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCaa                         >  1:319443/1‑148 (MQ=255)
                                                                                                                      tgtgGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCaa                         <  1:2516316/148‑1 (MQ=255)
                                                                                                                         gggTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATa                      <  2:2561146/148‑1 (MQ=255)
                                                                                                                          ggTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATaa                     >  1:1332965/1‑148 (MQ=255)
                                                                                                                            tCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAAcc                   <  1:2155984/148‑1 (MQ=255)
                                                                                                                            tCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAAcc                   >  1:2340394/1‑148 (MQ=255)
                                                                                                                             cTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAAccc                  >  1:1001515/1‑148 (MQ=255)
                                                                                                                             cTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGAAACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTAGCCGAACGCCGCGATCCCATCGTGGAAGAGAACTGCGAGCACGTCCATTACATCAAATAAccc                  >  1:1053379/1‑148 (MQ=255)
                                                                                                                               gCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTc                >  2:1310538/1‑148 (MQ=255)
                                                                                                                                ccGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTcc               <  2:2526874/148‑1 (MQ=255)
                                                                                                                                   tgTCAAAGTGTTTGGCCCGCTGATTGTATCTTTTGATACCCACGTCAACAACCTGATAGACGAAAAAAAAAATCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCgg            <  1:909484/147‑1 (MQ=255)
                                                                                                                                   ggTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCAGTACATCAAATAACCCTCCCgg            >  2:899947/1‑148 (MQ=255)
                                                                                                                                      cAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGgaga         <  1:3337916/148‑1 (MQ=255)
                                                                                                                                       aaaGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGagag        <  2:3532581/148‑1 (MQ=255)
                                                                                                                                       aaaGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGagag        <  2:1323668/148‑1 (MQ=255)
                                                                                                                                        aaGAGTTATGCCCGATGATAGTCTCTATTGATACCCACTGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGACGAACGCAGCGATCACATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAgg       >  2:183142/1‑148 (MQ=255)
                                                                                                                                         agagTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGc      <  2:1052675/148‑1 (MQ=255)
                                                                                                                                           agTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTc    >  2:1692508/1‑148 (MQ=255)
                                                                                                                                           agTTAGGCCCGCTGATTGTCTCGATTGATACCCACGTCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCAGTACATCAAATAACCCTCCCGGAGAGGCTc    <  2:2780257/148‑1 (MQ=255)
                                                                                                                                             ttCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTCAc  >  1:1527809/1‑148 (MQ=255)
                                                                                                                                               |                                                                                                                                                 
TGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTCAC  >  CP009273/3201066‑3201354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTCACCCCT  >  CP009273/3201060‑3201358
                                                                                                                                                     |                                                                                                                                                     
GAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTA                                                                                                                                                       <  SRR19973067.1880695/150‑1 (MQ=60)
GAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTA                                                                                                                                                       <  SRR19973067.3431260/150‑1 (MQ=60)
  AGTCTGGCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGATGAGATTGATGAAGTGCACTGGACAGCGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGG                                                                                                                                                     <  SRR19973067.596936/150‑1 (MQ=60)
  AGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGG                                                                                                                                                     <  SRR19973067.2306285/150‑1 (MQ=60)
      TGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCG                                                                                                                                                 <  SRR19973067.629755/150‑1 (MQ=60)
            AAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATT                                                                                                                                           >  SRR19973067.1782963/1‑150 (MQ=60)
                TCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCT                                                                                                                                       <  SRR19973067.3186803/150‑1 (MQ=60)
                TCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCT                                                                                                                                       <  SRR19973067.3188174/150‑1 (MQ=60)
                TCAAGGCGCTACATGTGATTGTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCT                                                                                                                                       <  SRR19973067.2700073/150‑1 (MQ=60)
                  AAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCT                                                                                                                                     <  SRR19973067.512917/150‑1 (MQ=60)
                   AGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTA                                                                                                                                    >  SRR19973067.1761991/1‑150 (MQ=60)
                    GGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTAT                                                                                                                                   <  SRR19973067.899491/150‑1 (MQ=60)
                         TACATGTGATGTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATA                                                                                                                              <  SRR19973067.2435584/150‑1 (MQ=60)
                           CATGTGATTTTCCCGGCTGGCTGCGCGGTTCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACC                                                                                                                            <  SRR19973067.2735196/150‑1 (MQ=60)
                           CATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACC                                                                                                                            >  SRR19973067.3030704/1‑150 (MQ=60)
                           CATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACC                                                                                                                            >  SRR19973067.3105914/1‑150 (MQ=60)
                           CATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTACCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACC                                                                                                                            >  SRR19973067.526015/1‑150 (MQ=60)
                            ATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCC                                                                                                                           >  SRR19973067.3064182/1‑150 (MQ=60)
                             TGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCA                                                                                                                          >  SRR19973067.1010630/1‑150 (MQ=60)
                             TGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCA                                                                                                                          >  SRR19973067.2798205/1‑150 (MQ=60)
                             TGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCA                                                                                                                          <  SRR19973067.1281442/150‑1 (MQ=60)
                                 ATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGC                                                                                                                      >  SRR19973067.2403073/1‑150 (MQ=60)
                                 ATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGC                                                                                                                      >  SRR19973067.2403070/1‑150 (MQ=60)
                                    TTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAAC                                                                                                                   >  SRR19973067.3456133/1‑150 (MQ=60)
                                    TTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAAC                                                                                                                   >  SRR19973067.3456258/1‑150 (MQ=60)
                                           CAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGA                                                                                                            >  SRR19973067.3178598/1‑150 (MQ=60)
                                            AGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGAT                                                                                                           >  SRR19973067.1745906/1‑150 (MQ=60)
                                            AGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGAT                                                                                                           >  SRR19973067.3381014/1‑150 (MQ=60)
                                            AGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGAT                                                                                                           >  SRR19973067.435243/1‑150 (MQ=60)
                                                       TGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACA                                                                                                <  SRR19973067.1423175/150‑1 (MQ=60)
                                                         CTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGGTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAA                                                                                              >  SRR19973067.1438800/1‑150 (MQ=60)
                                                                 AACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTT                                                                                      >  SRR19973067.986016/1‑150 (MQ=60)
                                                                  ACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTC                                                                                     <  SRR19973067.1522439/150‑1 (MQ=60)
                                                                  ACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTC                                                                                     <  SRR19973067.1522522/150‑1 (MQ=60)
                                                                   CCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGTCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCG                                                                                    <  SRR19973067.1163302/150‑1 (MQ=60)
                                                                   CCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGTCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCG                                                                                    <  SRR19973067.3435295/150‑1 (MQ=60)
                                                                   CCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCG                                                                                    >  SRR19973067.2188345/1‑150 (MQ=60)
                                                                       GGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGGTACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGA                                                                                <  SRR19973067.608697/150‑1 (MQ=60)
                                                                       GGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGA                                                                                <  SRR19973067.608097/150‑1 (MQ=60)
                                                                         TGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAAC                                                                              <  SRR19973067.1229396/150‑1 (MQ=60)
                                                                         TGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAAC                                                                              <  SRR19973067.2828607/150‑1 (MQ=60)
                                                                          GGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACG                                                                             <  SRR19973067.225247/150‑1 (MQ=60)
                                                                          GGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACG                                                                             <  SRR19973067.2033021/150‑1 (MQ=60)
                                                                          GGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACG                                                                             <  SRR19973067.2032970/150‑1 (MQ=60)
                                                                              GAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGC                                                                         >  SRR19973067.571707/1‑150 (MQ=60)
                                                                               AGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCG                                                                        <  SRR19973067.89286/150‑1 (MQ=60)
                                                                                            GCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGA                                                           <  SRR19973067.2861586/150‑1 (MQ=60)
                                                                                            GCACGGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGA                                                           <  SRR19973067.2861583/150‑1 (MQ=60)
                                                                                             CACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAA                                                          >  SRR19973067.1904118/1‑150 (MQ=60)
                                                                                                  GACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGAT                                                     >  SRR19973067.918589/1‑150 (MQ=60)
                                                                                                   ACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATC                                                    <  SRR19973067.968888/150‑1 (MQ=60)
                                                                                                   ACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATC                                                    <  SRR19973067.77305/150‑1 (MQ=60)
                                                                                                   ACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATC                                                    <  SRR19973067.77311/150‑1 (MQ=60)
                                                                                                    CAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCT                                                   >  SRR19973067.200773/1‑150 (MQ=60)
                                                                                                       AGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCG                                                >  SRR19973067.1894604/1‑150 (MQ=60)
                                                                                                       AGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCG                                                >  SRR19973067.1894587/1‑150 (MQ=60)
                                                                                                         CTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAG                                              <  SRR19973067.419977/150‑1 (MQ=60)
                                                                                                         CTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAG                                              >  SRR19973067.2448089/1‑150 (MQ=60)
                                                                                                         CTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAG                                              <  SRR19973067.420855/150‑1 (MQ=60)
                                                                                                          TCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGC                                             >  SRR19973067.437955/1‑150 (MQ=60)
                                                                                                            GGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCAC                                           >  SRR19973067.264901/1‑150 (MQ=60)
                                                                                                            GGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCAC                                           >  SRR19973067.264938/1‑150 (MQ=60)
                                                                                                            GGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCAC                                           >  SRR19973067.264953/1‑150 (MQ=60)
                                                                                                            GGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCAGCGAGCAC                                           >  SRR19973067.1229504/1‑150 (MQ=60)
                                                                                                              AATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGT                                         >  SRR19973067.3106480/1‑150 (MQ=60)
                                                                                                                    GGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTA                                   >  SRR19973067.1073634/1‑150 (MQ=60)
                                                                                                                    GGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTA                                   >  SRR19973067.375197/1‑150 (MQ=60)
                                                                                                                    GGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTA                                   <  SRR19973067.2034572/150‑1 (MQ=60)
                                                                                                                    GGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTA                                   >  SRR19973067.1073624/1‑150 (MQ=60)
                                                                                                                       GTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACAT                                <  SRR19973067.1045917/150‑1 (MQ=60)
                                                                                                                       GTCACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACAT                                <  SRR19973067.175203/150‑1 (MQ=60)
                                                                                                                          ACTGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAA                             >  SRR19973067.321602/1‑150 (MQ=60)
                                                                                                                            TGTGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAAT                           <  SRR19973067.2532814/150‑1 (MQ=60)
                                                                                                                              TGGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAA                         >  SRR19973067.1342061/1‑150 (MQ=60)
                                                                                                                               GGGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAAC                        <  SRR19973067.1214598/150‑1 (MQ=60)
                                                                                                                                GGTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACC                       >  SRR19973067.2355705/1‑150 (MQ=60)
                                                                                                                                 GTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCC                      >  SRR19973067.1008398/1‑150 (MQ=60)
                                                                                                                                 GTCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGAAACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTAGCCGAACGCCGCGATCCCATCGTGGAAGAGAACTGCGAGCACGTCCATTACATCAAATAACCC                      >  SRR19973067.1060600/1‑150 (MQ=60)
                                                                                                                                  TCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCT                     <  SRR19973067.477874/150‑1 (MQ=60)
                                                                                                                                  TCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCT                     <  SRR19973067.2170586/150‑1 (MQ=60)
                                                                                                                                  TCTGCCGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCT                     <  SRR19973067.2170400/150‑1 (MQ=60)
                                                                                                                                       CGGGTCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGG                >  SRR19973067.1683272/1‑150 (MQ=60)
                                                                                                                                         TGTCAAAGTGTTTGGCCCGCTGATTGTATCTTTTGATACCCACGTCAACAACCTGATAGACGAAAAAAAAAATCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAG              <  SRR19973067.915519/150‑1 (MQ=60)
                                                                                                                                           TCAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAG            >  SRR19973067.1447735/1‑150 (MQ=60)
                                                                                                                                            CAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGG           <  SRR19973067.3359079/150‑1 (MQ=60)
                                                                                                                                            CAAAGAGTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGG           <  SRR19973067.3359051/150‑1 (MQ=60)
                                                                                                                                                 AGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTCAC      >  SRR19973067.1537961/1‑150 (MQ=60)
                                                                                                                                                 AGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTCAC      >  SRR19973067.1538098/1‑150 (MQ=60)
                                                                                                                                                  GTTAGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGTCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCGCACC     >  SRR19973067.304669/1‑150 (MQ=60)
                                                                                                                                                     AGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTCACCCCT  >  SRR19973067.1593688/1‑150 (MQ=60)
                                                                                                                                                     AGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTCACCCCT  <  SRR19973067.2157969/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
GAAGGCTGCCAGAAATTCAAGGCGCTACATGTGATTTTCCCGGCAGGCTGCGCGGTGCTGGCGGCAACCCAGGTGGAAGAGATTGAAGAAGTGCACTGGACAGAGCTCGGAATGCCGGAGTCACTGTGGGTCTGCCGGGTCAAAGAGTTCGGCCCGCTGATTGTCTCTATTGATACCCACGGCAACAACCTGATAGCCGAAAACAAAAAGCTGTTCGCCGAACGCCGCGATCCCATCGTGGAAGAGATCTGCGAGCACGTCCATTACATCAAATAACCCTCCCGGAGAGGCTCACCCCT  >  CP009273/3201060‑3201358

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑