Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,203,599 A→T 42.9% W102R (TGG→AGG)  tsaD ← tRNA(ANN) t(6)A37 threonylcarbamoyladenosine modification protein; glycation binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,203,5990AT42.9% 143.9 / inf 249W102R (TGG→AGG) tsaDtRNA(ANN) t(6)A37 threonylcarbamoyladenosine modification protein; glycation binding protein
Reads supporting (aligned to +/- strand):  ref base A (76/66);  new base T (50/57);  total (126/123)
Fisher's exact test for biased strand distribution p-value = 3.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 249 total aligned reads displayed.

TACTGACCAATGCCAGTCACGCTGATTAACTGCGTATGACCGCCGGAAACAAGCAGCGCAACAAACGGAAATTCCGGCGGGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCAGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCTGCGGTATAGGCCACAGCATCAATGTCTTTTGCCGTTAAACCAGACTCCTTTAGCGCCGCCTGGATCAACGGTACGGTTTTACG  >  CP009273/3203451‑3203746
                                                                                                                                                    |                                                                                                                                                   
tACTGACCAATGCCAGTCACGCTGATTAACTGCGTATGACCGCCGGAAACAAGCAGCGCAACAAACGGAAATTCCGGCGGGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCAg                                                                                                                                                    >  2:2986339/1‑150 (MQ=255)
 aCTGACCAATGCCAGTCACGCTGATTAACTGCGTATGACCGCCGGAAACAAGCAGCGCAACAAACGGAAATTCCGGCGGGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCTgg                                                                                                                                                   <  2:3580502/150‑1 (MQ=255)
      ccAATGCCAGTCACGCTGATTAACTGCGTATGACCGCCGGAAACAAGCAGCGCAACAAACGGAAATTCCGGCGGGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCAGGCAAAc                                                                                                                                              <  1:3783711/150‑1 (MQ=255)
        aaTGCCAGTCACGCTGATTAACTGCGTATGACCGCCGGAAACAAGCAGCGCAACAAACGGAAATTCCGGCGGGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCTGGCAAACGc                                                                                                                                            >  1:4096955/1‑150 (MQ=255)
          tGCCAGTCACGCTGATTAACTGCGTATGACCGCCGGAAACAAGCAGCGCAACAAACGGAAATTCCGGCGGGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCTGGCAAACGCCa                                                                                                                                          >  1:1970306/1‑150 (MQ=255)
            ccAGTCACGCTGATTAACTGCGTATGACCGCCGGAAACAAGCAGCGCAACAAACGGAAATTCCGGCGGGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCTGGCAAACGCCaga                                                                                                                                        >  1:582227/1‑150 (MQ=255)
            ccAGTCACGCTGATTAACTGCGTATGACCGCCGGAAACAAGCAGCGCAACAAACGGAAATTCCGGCGGGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCTGGCAAACGCCaga                                                                                                                                        >  1:582231/1‑150 (MQ=255)
             cAGTCACGCTGATTAACTGCGTATGACCGCCGGAAACAAGCAGCGCAACAAACGGAAATTCCGGCGGGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCTGGCAAACGCCagag                                                                                                                                       >  2:3838740/1‑150 (MQ=255)
             cAGTCACGCTGATTAACTGCGTATGACCGCCGGAAACAAGCAGCGCAACAAACGGAAATTCCGGCGGGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCTGGCAAACGCCagag                                                                                                                                       <  2:518602/150‑1 (MQ=255)
             cAGTCACGCTGATTAACTGCGTATGACCGCCGGAAACAAGCAGCGCAACAAACGGAAATTCCGGCGGGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCAGGCAAACGCCagag                                                                                                                                       >  1:966642/1‑150 (MQ=255)
               gTCACGCTGATTAACTGCGTATGACCGCCGGAAACAAGCAGCGCAACAAACGGAAATTCCGGCGGGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCAGGCAAACGCCAGAGaa                                                                                                                                     >  2:2648723/1‑150 (MQ=255)
                tCACGCTGATTAACTGCGTATGACCGCCGGAAACAAGCAGCGCAACAAACGGAAATTCCGGCGGGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCTGGCAAACGCCAGAGAAc                                                                                                                                    <  1:799451/150‑1 (MQ=255)
                tCACGCTGATTAACTGCGTATGACCGCCGGAAACAAGCAGCGCAACAAACGGAAATTCCGGCGGGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCAGGCAAACGCCAGAGAAc                                                                                                                                    >  1:3905360/1‑150 (MQ=255)
                tCACGCTGATTAACTGCGTATGACCGCCGGAAACAAGCAGCGCAACAAACGGAAATTCCGGCGGGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCAGGCAAACGCCAGAGAAc                                                                                                                                    >  1:3905254/1‑150 (MQ=255)
                 cACGCTGATTAACTGCGTATGACCGCCGGAAACAAGCAGCGCAACAAACGGAAATTCCGGCGGGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCAGGCAAACGCCAGAGAACg                                                                                                                                   >  2:3549081/1‑150 (MQ=255)
                  aCGCTGATTAACTGCGTATGACCGCCGGAAACAAGCAGCGCAACAAACGGAAATTCCGGCGGGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCTGGCAAACGCCAGAGAACGc                                                                                                                                  >  1:1734256/1‑150 (MQ=255)
                    gCTGATTAACTGCGTATGACCGCCGGAAACAAGCAGCGCAACAAACGGAAATTCCGGCGGGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCTGGCAAACGCCAGAGAACGccc                                                                                                                                <  1:3108707/150‑1 (MQ=255)
                     cTGATTAACTGCGTATGACCGCCGGAAACAAGCAGCGCAACAAACGGAAATTCCGGCGGGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCTGGCAAACGCCAGAGAACGcccc                                                                                                                               >  1:2270930/1‑150 (MQ=255)
                       gATTAACTGCGTATGACCGCCGGAAACAAGCAGCGCAACAAACGGAAATTCCGGCGGGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCAGGCAAACGCCAGAGAACGCCCCAc                                                                                                                             >  2:2690503/1‑150 (MQ=255)
                        aTTAACTGCGTATGACCGCCGGAAACAAGCAGCGCAACAAACGGAAATTCCGGCGGGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCTGGCAAACGCCAGAGAACGCCCCACg                                                                                                                            >  2:1324118/1‑150 (MQ=255)
                         ttAACTGCGTATGACCGCCGGAAACAAGCAGCGCAACAAACGGAAATTCCGGCGGGATATCTACCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGAACAGGGATCTCCGGAACGACCCTGGCAAACGCCAGAGAACGCCCCACgg                                                                                                                           >  1:433253/1‑150 (MQ=255)
                            aCTGCGTATGACCGCCGGAAACAATCAGCGCAACAAACGGAAATTCCGGCGGGTTATCTGCCAGCATCGGCGCGAACATATGCCCTTCCCAATGGTGCAAAGGGATCGCCGGATCGTCCCTGGCAAACGCCAGAGAACGCCCCACGGTcg                                                                                                                        <  1:2345034/150‑1 (MQ=255)
                                cGTATGACCGCCGGAAACAAGCAGCGCAACAAACGGAAATTCCGGCGGGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCAGGCAAACGCCAGAGAACGCCCCACGGTCGCGcc                                                                                                                    >  1:2650805/1‑150 (MQ=255)
                                   aTGACCGCCGGAAACAAGCAGCGCAACAAACGGAAATTCCGGCGGGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCAGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAAc                                                                                                                 >  1:913978/1‑150 (MQ=255)
                                     gACCGCCGGAAACAAGCAGCGCAACAAACGGAAATTCCGGCGGGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCTGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCa                                                                                                               >  1:2212151/1‑150 (MQ=255)
                                           cgGAAACAAGCAGCGCAACAAACGGAAATTCCGGCGGGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCTGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAgcg                                                                                                         >  1:3458745/1‑150 (MQ=255)
                                           cgGAAACAAGCAGCGCAACAAACGGAAATTCCGGCGGGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCTGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAgcg                                                                                                         >  2:3091688/1‑150 (MQ=255)
                                             gAAACAAGCAGCGCAACAAACGGAAATTCCGGCGGGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCTGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAgcgcg                                                                                                       >  2:1948053/1‑150 (MQ=255)
                                                  aaGCAGCGCAACAAACGGAAATTCCGGCGGGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCTGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGAc                                                                                                  >  1:2028789/1‑150 (MQ=255)
                                                   agcagcGCAACACACGGAAATTCCGGCGGGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCAGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACt                                                                                                 <  2:3946556/150‑1 (MQ=255)
                                                   agcagcGCAACAAACGGAAATTCCGGCGGGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCTGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACt                                                                                                 >  2:3669699/1‑150 (MQ=255)
                                                   agcagcGCAACAAACGGAAATTCCGGCGGGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCAGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACt                                                                                                 >  2:1013896/1‑150 (MQ=255)
                                                      agcGCAACAAACGGAAATTCCGGCGGGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCAGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAAt                                                                                              >  2:2409950/1‑150 (MQ=255)
                                                      agcGCAACAAACGGAAATTCCGGCGGGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCAGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAAt                                                                                              <  1:4163112/150‑1 (MQ=255)
                                                      agcGCAACAAACGGAAATTCCGGCGGGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCAGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAAt                                                                                              <  2:1798949/150‑1 (MQ=255)
                                                        cgcAACAAACGGAAATTCCGGCGGGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCTGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATcc                                                                                            <  2:1130517/150‑1 (MQ=255)
                                                        cgcAACAAACGGAAATTCCGGCGGGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCTGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATcc                                                                                            <  1:3747428/150‑1 (MQ=255)
                                                        cgcAACAAACGGAAATTCCGGCGGGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCAGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATcc                                                                                            >  2:2208239/1‑150 (MQ=255)
                                                           aacaaACGGAAATTCCGGCGGGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCTGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAgg                                                                                         <  1:2305527/150‑1 (MQ=255)
                                                              aaaCGGAAATTCCGGCGGGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCAGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGcc                                                                                      >  2:2952026/1‑150 (MQ=255)
                                                               aaCGGAAATTCCGGCGGGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCTGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCt                                                                                     <  1:4220871/150‑1 (MQ=255)
                                                               aaCGGAAATTCCGGCGGGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCTGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCt                                                                                     <  1:2597529/150‑1 (MQ=255)
                                                               aaCGGAAATTCCGGCGGGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCTGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCt                                                                                     <  1:2597044/150‑1 (MQ=255)
                                                                aCGGAAATTCCGGCGGGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCAGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCTg                                                                                    <  1:4381193/150‑1 (MQ=255)
                                                                   gAAATTCCGGCGGGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCTGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCTGCgg                                                                                 <  2:3907810/150‑1 (MQ=255)
                                                                   gAAATTCCGGCGGGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCTGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCTGCgg                                                                                 <  2:1584276/150‑1 (MQ=255)
                                                                     aaTTCCGGCGGGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCTGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCTGCGGta                                                                               >  2:3421808/1‑150 (MQ=255)
                                                                     aaTTCCGGCGGGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCTGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCTGCGGta                                                                               >  2:2779135/1‑150 (MQ=255)
                                                                      aTTCCGGCGGGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCTGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCTGCGGtat                                                                              >  1:3666436/1‑150 (MQ=255)
                                                                      aTTCCGGCGGGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCAGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCTGCGGtat                                                                              <  2:3099656/150‑1 (MQ=255)
                                                                       ttCCGGCGGGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCAGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCTGCGGtata                                                                             <  2:532163/150‑1 (MQ=255)
                                                                         ccGGCGGGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCAGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCTGCGGTATAgg                                                                           >  1:2490097/1‑150 (MQ=255)
                                                                          cggcggGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCTGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCTGCGGTATAGGc                                                                          >  2:1580170/1‑150 (MQ=255)
                                                                          cggcggGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCAGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCTGCGGTATAGGc                                                                          >  2:4566893/1‑150 (MQ=255)
                                                                            gcggGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCAGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCTGCGGTATAGGCca                                                                        >  2:3880370/1‑150 (MQ=255)
                                                                             cggGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCTGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCTGCGGTATAGGCcac                                                                       <  2:3755707/150‑1 (MQ=255)
                                                                              gggTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCTGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCTGCGGTATAGGCcaca                                                                      >  1:2110827/1‑150 (MQ=255)
                                                                              gggTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCAGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCTGCGGTATAGGCcaca                                                                      >  2:689855/1‑150 (MQ=255)
                                                                                 ttATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCTGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCTGCGGTATAGGCCACAGCa                                                                   >  2:1556839/1‑150 (MQ=255)
                                                                                 ttATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCTGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCTGCGGTATAGGCCACAGCa                                                                   <  2:1874511/150‑1 (MQ=255)
                                                                                  tATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCAGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCTGCGGTATAGGCCACAGCAt                                                                  >  2:1130051/1‑150 (MQ=255)
                                                                                  tATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCAGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCTGCGGTATAGGCCACAGCAt                                                                  >  2:2376833/1‑150 (MQ=255)
                                                                                   aTCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCAGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCTGCGGTATAGGCCACAGCATc                                                                 <  2:4283422/150‑1 (MQ=255)
                                                                                    tCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCTGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCTGCGGTATAGGCCACAGCATCa                                                                <  1:3965658/150‑1 (MQ=255)
                                                                                    tCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCTGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCTGCGGTATAGGCCACAGCATCa                                                                <  1:3585476/150‑1 (MQ=255)
                                                                                      ttCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCTGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCTGCGGTATAGGCCACAGCATCAAt                                                              <  2:864566/150‑1 (MQ=255)
                                                                                      ttCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCTGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCTGCGGTATAGGCCACAGCATCAAt                                                              <  2:864265/150‑1 (MQ=255)
                                                                                       tCCAGCATCGGCGCTAACAGATGCCCTTCCAGATGGTGTACAGGGATCGCCGGAACGTCCCAGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCTGCGGTATAGGCCACAGCATCAATg                                                             >  2:829319/1‑150 (MQ=255)
                                                                                            cATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCAGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCTGCGGTATAGGCCACAGCATCAATGTCttt                                                        <  1:4489035/150‑1 (MQ=255)
                                                                                             aTCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCTGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCTGCGGTATAGGCCACAGCATCAATGTCtttt                                                       <  2:3047266/150‑1 (MQ=255)
                                                                                                 gcgcTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCAGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCTGCGGTATAGGCCACAGCATCAATGTCTTTTGCCg                                                   >  1:2851392/1‑150 (MQ=255)
                                                                                                 gcgcTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCAGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCTGCGGTATAGGCCACAGCATCAATGTCTTTTGCCg                                                   <  1:3310529/150‑1 (MQ=255)
                                                                                                   gcTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCTGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCTGCGGTATAGGCCACAGCATCAATGTCTTTTGCCGtt                                                 >  2:212665/1‑150 (MQ=255)
                                                                                                   gcTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCAGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCTGCGGTATAGGCCACAGCATCAATGTCTTTTGCCGtt                                                 <  2:639050/150‑1 (MQ=255)
                                                                                                    cTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCAGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCTGCGGTATAGGCCACAGCATCAATGTCTTTTGCCGTTa                                                >  1:2361090/1‑150 (MQ=255)
                                                                                                     tAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCAGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCTGCGGTATAGGCCACAGCATCAATGTCTTTTGCCGTTaa                                               <  2:3181284/150‑1 (MQ=255)
                                                                                                     tAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCAGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCTGCGGTATAGGCCACAGCATCAATGTCTTTTGCCGTTaa                                               >  1:333071/1‑150 (MQ=255)
                                                                                                        cAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCAGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCTGCGGTATAGGCCACAGCATCAATGTCTTTTGCCGTTAAAcc                                            <  2:2534782/150‑1 (MQ=255)
                                                                                                           aTGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCAGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCTGCGGTATAGGCCACAGCATCAATGTCTTTTGCCGTTAAACCAGa                                         <  1:4019681/150‑1 (MQ=255)
                                                                                                           aTGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCAGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCTGCGGTATAGGCCACAGCATCAATGTCTTTTGCCGTTAAACCAGa                                         >  2:4564204/1‑150 (MQ=255)
                                                                                                              cccTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCTGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCTGCGGTATAGGCCACAGCATCAATGTCTTTTGCCGTTAAACCAGACTc                                      <  2:1222933/150‑1 (MQ=255)
                                                                                                               ccTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCTGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCTGCGGTATAGGCCACAGCATCAATGTCTTTTGCCGTTAAACCAGACTcc                                     <  2:406867/150‑1 (MQ=255)
                                                                                                                cTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCAGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCTGCGGTATAGGCCACAGCATCAATGTCTTTTGCCGTTAAACCAGACTCCt                                    <  2:74855/150‑1 (MQ=255)
                                                                                                                cTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCAGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCTGCGGTATAGGCCACAGCATCAATGTCTTTTGCCGTTAAACCAGACTCCt                                    <  1:4274037/150‑1 (MQ=255)
                                                                                                                     atatGGTGTACAGGGATCGCCGGAACGTCCCAGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCTGCGGTATAGGCCACAGCATCAATGTCTTTTGCCGTTAAACCAGACTCCTTTAgc                               >  2:2573129/1‑150 (MQ=255)
                                                                                                                       atGGTGTACAGGGATCGCCGGAACGTCCCAGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCTGCGGTATAGGCCACAGCATCAATGTCTTTTGCCGTTAAACCAGACTCCTTTAgcgc                             >  1:1011008/1‑150 (MQ=255)
                                                                                                                           tgtACAGGGATCGCCGGAACGTCCCTGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCTGCGGTATAGGCCACAGCATCAATGTCTTTTGCCGTTAAACCAGACTCCTTTAGCgccgcc                         <  1:1367701/150‑1 (MQ=255)
                                                                                                                           tgtACAGGGATCGCCGGAACGTCCCAGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCTGCGGTATAGGCCACAGCATCAATGTCTTTTGCCGTTAAACCAGACTCCTTTAGCgccgcc                         <  2:4476116/150‑1 (MQ=255)
                                                                                                                                   gATCGCCGGAACGTCCCTGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCTGCGGTATAGGCCACAGCATCAATGTCTTTTGCCGTTAAACCAGACTCCTTTAGCGCCGCCTGGATCaa                 >  2:1469682/1‑150 (MQ=255)
                                                                                                                                   gATCGCCGGAACGTCCCAGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCTGCGGTATAGGCCACAGCATCAATGTCTTTTGCCGTTAAACCAGACTCCTTTAGCGCCGCCTGGATCaa                 <  2:3905254/150‑1 (MQ=255)
                                                                                                                                     tCGCCGGAACGTCCCTGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCTGCGGTATAGGCCACAGCATCAATGTCTTTTGCCGTTAAACCAGACTCCTTTAGCGCCGCCTGGATCAacg               >  2:3911276/1‑150 (MQ=255)
                                                                                                                                     tCGCCGGAACGTCCCAGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCTGCGGTATAGGCCACAGCATCAATGTCTTTTGCCGTTAAACCAGACTCCTTTAGCGCCGCCTGGATCAacg               >  2:4187475/1‑150 (MQ=255)
                                                                                                                                       gCCGGAACGTCCCAGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCTGCGGTATAGGCCACAGCATCAATGTCTTTTGCCGTTAAACCAGACTCCTTTAGCGCCGCCTGGATCAacggt             <  2:2287895/150‑1 (MQ=255)
                                                                                                                                       gCCGGAACGTCCCAGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCTGCGGTATAGGCCACAGCATCAATGTCTTTTGCCGTTAAACCAGACTCCTTTAGCGCCGCCTGGATCAacggt             <  2:1651338/150‑1 (MQ=255)
                                                                                                                                        ccGGAACGTCCCAGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCTGCGGTATAGGCCACAGCATCAATGTCTTTTGCCGTTAAACCAGACTCCTTTAGCGCCGCCTGGATCAacggta            <  1:4243637/150‑1 (MQ=255)
                                                                                                                                         cGGAACGTCCCAGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCTGCGGTATAGGCCACAGCATCAATGTCTTTTGCCGTTAAACCAGACTCCTTTAGCGCCGCCTGGATCAacggtac           <  1:2901830/150‑1 (MQ=255)
                                                                                                                                          ggAACGTCCCAGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCTGCGGTATAGGCCACAGCATCAATGTCTTTTGCCGTTAAACCAGACTCCTTTAGCGCCGCCTGGATCAacggtacg          <  1:1668585/150‑1 (MQ=255)
                                                                                                                                            aaCGTCCCAGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCTGCGGTATAGGCCACAGCATCAATGTCTTTTGCCGTTAAACCAGACTCCTTTAGCGCCGCCTGGATCAacggtacggt        >  2:3966117/1‑150 (MQ=255)
                                                                                                                                            aaCGTCCCAGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCTGCGGTATAGGCCACAGCATCAATGTCTTTTGCCGTTAAACCAGACTCCTTTAGCGCCGCCTGGATCAacggtacggt        >  1:1285230/1‑150 (MQ=255)
                                                                                                                                                  ccAGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCTGCGGTATAGGCCACAGCATCAATGTCTTTTGCCGTTAAACCAGACTCCTTTAGCGCCGCCTGGATCAACGGTACGGTTTTACg  >  2:1265828/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TACTGACCAATGCCAGTCACGCTGATTAACTGCGTATGACCGCCGGAAACAAGCAGCGCAACAAACGGAAATTCCGGCGGGTTATCTTCCAGCATCGGCGCTAACAGATGCCCTTCCATATGGTGTACAGGGATCGCCGGAACGTCCCAGGCAAACGCCAGAGAACGCCCCACGGTCGCGCCAACCAGTAGCGCGCCGACTAATCCAGGGCCTGCGGTATAGGCCACAGCATCAATGTCTTTTGCCGTTAAACCAGACTCCTTTAGCGCCGCCTGGATCAACGGTACGGTTTTACG  >  CP009273/3203451‑3203746

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A