Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,277,142 C→A 42.3% P214Q (CCG→CAG)  kbaY → tagatose 6‑phosphate aldolase 1, kbaY subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,277,1420CA42.3% 222.8 / 242.9 222P214Q (CCG→CAG) kbaYtagatose 6‑phosphate aldolase 1, kbaY subunit
Reads supporting (aligned to +/- strand):  ref base C (68/60);  new base A (50/44);  total (118/104)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 225 total aligned reads displayed.

AACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCCGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTGAACGTTGCCACAGAATTAAAAATAGCCTTCGCTGGCGCGGTTAAAGCCTGGTTTGCGGAAAATCCGCAGGGTAATGATCCTCGTTATTATATGCGCGTCGG  >  CP009273/3276995‑3277289
                                                                                                                                                   |                                                                                                                                                   
aaCTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTcc                                                                                                                                                     <  2:3255737/148‑1 (MQ=255)
  ctgactgGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCCgg                                                                                                                                                   >  1:382403/1‑148 (MQ=255)
   tgactgGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCCGGa                                                                                                                                                  >  2:2020349/1‑148 (MQ=255)
      ctgGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCCGGATGa                                                                                                                                               <  1:732140/148‑1 (MQ=255)
       tgGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCAGGATGaa                                                                                                                                              <  1:1797141/148‑1 (MQ=255)
       tgGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCAGGATGaa                                                                                                                                              <  2:2200531/148‑1 (MQ=255)
        ggCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCCGGATGAAt                                                                                                                                             <  2:3160596/148‑1 (MQ=255)
        ggCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCCGGATGAAt                                                                                                                                             <  1:2054616/148‑1 (MQ=255)
           gTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCCGGATGAATTTg                                                                                                                                          <  2:1408135/148‑1 (MQ=255)
            tCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCCGGATGAATTTGt                                                                                                                                         <  2:1124242/148‑1 (MQ=255)
            tCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCAGGATGAATTTGt                                                                                                                                         <  1:879628/148‑1 (MQ=255)
               aCAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCCGGATGAATTTGTCCg                                                                                                                                      <  2:2195038/148‑1 (MQ=255)
                  tccTGGAGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCAGGATGAATTTGTCCGTCg                                                                                                                                   >  2:938557/2‑148 (MQ=255)
                   ccTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCAGGATGAATTTGTCCGTCGc                                                                                                                                  >  1:795506/1‑148 (MQ=255)
                    cTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCAGGATGAATTTGTCCGTCGCa                                                                                                                                 >  1:2470653/1‑148 (MQ=255)
                     tGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCAGGATGAATTTGTCCGTCGCAc                                                                                                                                >  2:3074660/1‑148 (MQ=255)
                     tGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGAGGTTCAGGATGAATTTGTCCGTCGCAc                                                                                                                                >  2:235616/1‑148 (MQ=255)
                         ggTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTTCATGGTGCCAGCGATGTTCAGGATGAATTTGTCCGTCGCACTAtt                                                                                                                            <  1:2281176/148‑1 (MQ=255)
                          gTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCCGGATGAATTTGTCCGTCGCACTATTg                                                                                                                           <  1:927256/148‑1 (MQ=255)
                           tAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCCGGATGAATTTGTCCGTCGCACTATTGa                                                                                                                          >  1:3255768/1‑148 (MQ=255)
                                 ttGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCAGGATGAATTTGTCCGTCGCACTATTGAACTTgg                                                                                                                    <  1:91754/148‑1 (MQ=255)
                                  tGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCAGGATGAATTTGTCCGTCGCACTATTGAACTTGGc                                                                                                                   >  1:516031/1‑148 (MQ=255)
                                  tGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCAGGATGAATTTGTCCGTCGCACTATTGAACTTGGc                                                                                                                   >  1:2668330/1‑148 (MQ=255)
                                    gTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCCGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGt                                                                                                                 <  1:1408869/148‑1 (MQ=255)
                                    gTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCAGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGt                                                                                                                 >  2:2873122/1‑148 (MQ=255)
                                          cgcACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCCGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACaaa                                                                                                           <  2:3100872/148‑1 (MQ=255)
                                             aCGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCCGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGt                                                                                                        <  2:2695049/148‑1 (MQ=255)
                                              cGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCCGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTg                                                                                                       <  2:1626651/148‑1 (MQ=255)
                                              cGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCAGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTg                                                                                                       <  2:473840/148‑1 (MQ=255)
                                              cGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCAGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTg                                                                                                       >  2:2753435/1‑148 (MQ=255)
                                               ggCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCCGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTGa                                                                                                      >  2:1008351/1‑148 (MQ=255)
                                               ggCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCCGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTGa                                                                                                      <  2:823414/148‑1 (MQ=255)
                                               ggCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCCGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTGa                                                                                                      <  2:1001677/148‑1 (MQ=255)
                                                  ttATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCAGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTGAACg                                                                                                   <  2:2552687/148‑1 (MQ=255)
                                                  ttATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCAGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTGAACg                                                                                                   <  1:304447/148‑1 (MQ=255)
                                                   tataCAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCCGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTGAACGt                                                                                                  <  2:2891434/148‑1 (MQ=255)
                                                   tataCAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCCGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTGAACGt                                                                                                  <  2:83552/148‑1 (MQ=255)
                                                    ataCAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCCGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTGAACGtt                                                                                                 >  2:665004/1‑148 (MQ=255)
                                                     taCAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCCGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTGAACGTTg                                                                                                <  1:2547435/148‑1 (MQ=255)
                                                     taCAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCAGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTGAACGTTg                                                                                                >  2:2440785/1‑148 (MQ=255)
                                                       cAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCCGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTGAACGTTGcc                                                                                              >  2:2804791/1‑148 (MQ=255)
                                                       cAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCCGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTGAACGTTGcc                                                                                              >  1:3165029/1‑148 (MQ=255)
                                                        aGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCCGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTGAACGTTGCca                                                                                             >  2:867531/1‑148 (MQ=255)
                                                            aaaCGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCAGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTGAACGTTGCCACAGa                                                                                         <  1:1488759/148‑1 (MQ=255)
                                                             aaCGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCCGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTGAACGTTGCCACAGaa                                                                                        >  2:2850670/1‑148 (MQ=255)
                                                             aaCGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCCGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTGAACGTTGCCACAGaa                                                                                        <  1:3029983/148‑1 (MQ=255)
                                                              aCGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCCGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTGAACGTTGCCACAGAAt                                                                                       >  1:498216/1‑148 (MQ=255)
                                                              aCGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCCGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTGAACGTTGCCACAGAAt                                                                                       >  2:1348441/1‑148 (MQ=255)
                                                               cGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCCGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTGAACGTTGCCACAGAAtt                                                                                      >  1:3243731/1‑148 (MQ=255)
                                                               cGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCAGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTGAACGTTGCCACAGAAtt                                                                                      <  2:2305878/148‑1 (MQ=255)
                                                                  cGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCAGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTGAACGTTGCCACAGAATTaaa                                                                                   >  2:3061505/1‑148 (MQ=255)
                                                                   gAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCAGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTGAACGTTGCCACAGAATTaaaa                                                                                  >  2:2124369/1‑148 (MQ=255)
                                                                    aaGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCCGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTGAACGTTGCCACAGACTTaaaaa                                                                                 >  2:1531017/1‑148 (MQ=255)
                                                                      gattgattTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCAGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTGAACGTTGCCACAGAATTAAAAATa                                                                               <  1:2843625/148‑1 (MQ=255)
                                                                      gattgattTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCAGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTGAACGTTGCCACAGAATTAAAAATa                                                                               >  1:1463251/1‑148 (MQ=255)
                                                                        ttgattTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCCGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTGAACGTTGCCACAGAATTAAAAATAGc                                                                             >  1:1414461/1‑148 (MQ=255)
                                                                        ttgattTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCAGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTGAACGTTGCCACAGAATTAAAAATAGc                                                                             >  1:3368392/1‑148 (MQ=255)
                                                                        ttgattTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCAGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTGAACGTTGCCACAGAATTAAAAATAGc                                                                             <  1:2157948/148‑1 (MQ=255)
                                                                             ttCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCCGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTGAACGTTGCCACAGAATTAAAAATAGCCTTCg                                                                        >  1:1174714/1‑148 (MQ=255)
                                                                             ttCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCAGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTGAACGTTGCCACAGAATTAAAAATAGCCTTCg                                                                        >  2:1780529/1‑148 (MQ=255)
                                                                                 aGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCCGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTGAACGTTGCCACAGAATTAAAAATAGCCTTCGCTgg                                                                    <  1:304027/148‑1 (MQ=255)
                                                                                  gCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCCGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTGAACGTTGCCACAGAATTAAAAATAGCCTTCGCTGgc                                                                   >  2:755384/1‑148 (MQ=255)
                                                                                   cGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCCGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTGAACGTTGCCACAGAATTAAAAATAGCCTTCGCTGgcg                                                                  >  2:2774401/1‑148 (MQ=255)
                                                                                     gCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCCGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTGAACGTTGCCACAGAATTAAAAATAGCCTTCGCTGGcgcg                                                                >  2:1494535/1‑148 (MQ=255)
                                                                                        ggCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCCGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTGAACGTTGCCACAGAATTAAAAATAGCCTTCGCTGGCGCGGtt                                                             >  1:2281960/1‑148 (MQ=255)
                                                                                        ggCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCAGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTGAACGTTGCCACAGAATTAAAAATAGCCTTCGCTGGCGCGGtt                                                             >  2:1945449/1‑148 (MQ=255)
                                                                                         gCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCCGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTGAACGTTGCCACAGAATTAAAAATAGCCTTCGCTGGCGCGGTTa                                                            >  1:1878347/1‑148 (MQ=255)
                                                                                         gCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCCGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTGAACGTTGCCACAGAATTAAAAATAGCCTTCGCTGGCGCGGTTa                                                            >  1:3473309/1‑148 (MQ=255)
                                                                                            gAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCCGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTGAACGTTGCCACAGAATTAAAAATAGCCTTCGCTGGCGCGGTTAAAg                                                         <  2:3005339/148‑1 (MQ=255)
                                                                                              aaTTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCAGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTGAACGTTGCCACAGAATTAAAAATAGCCTTCGCTGGCGCGGTTAAAGcc                                                       <  2:2252912/148‑1 (MQ=255)
                                                                                               aTTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCAGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTGAACGTTGCCACAGAATTAAAAATAGCCTTCGCTGGCGCGGTTAAAGCCt                                                      >  1:2337106/1‑148 (MQ=255)
                                                                                               aTTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCAGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTGAACGTTGCCACAGAATTAAAAATAGCCTTCGCTGGCGCGGTTAAAGCCt                                                      >  2:2745765/1‑148 (MQ=255)
                                                                                                ttCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCCGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTGAACGTTGCCACAGAATTAAAAATAGCCTTCGCTGGCGCGGTTAAAGCCTg                                                     >  1:3175992/1‑148 (MQ=255)
                                                                                                ttCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCAGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTGAACGTTGCCACAGAATTAAAAATAGCCTTCGCTGGCGCGGTTAAAGCCTg                                                     <  2:516031/148‑1 (MQ=255)
                                                                                                ttCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCAGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTGAACGTTGCCACAGAATTAAAAATAGCCTTCGCTGGCGCGGTTAAAGCCTg                                                     >  1:310975/1‑148 (MQ=255)
                                                                                                ttCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCAGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTGAACGTTGCCACAGAATTAAAAATAGCCTTCGCTGGCGCGGTTAAAGCCTg                                                     <  2:373126/148‑1 (MQ=255)
                                                                                                 tCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCCGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTGAACGTTGCCACAGAATTAAAAATAGCCTTCGCTGGCGCGGTTAAAGCCTgg                                                    >  2:2707547/1‑148 (MQ=255)
                                                                                                  cGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCCGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTGAACGTTGCCACAGAATTAAAAATAGCCTTCGCTGGCGCGGTTAAAGCCTGGt                                                   <  2:1065154/148‑1 (MQ=255)
                                                                                                      aaGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCAGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTGAACGTTGCCACAGAATTAAAAATAGCCTTCGCTGGCGCGGTTAAAGCCTGGTTTGc                                               >  1:1826962/1‑148 (MQ=255)
                                                                                                        gtggtgGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCAGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTGAACGTTGCCACAGAATTAAAAATAGCCTTCGCTGGCGCGGTTAAAGCCTGGTTTGCgg                                             >  1:2967783/1‑148 (MQ=255)
                                                                                                        gtggtgGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCAGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTGAACGTTGCCACAGAATTAAAAATAGCCTTCGCTGGCGCGGTTAAAGCCTGGTTTGCgg                                             <  1:3026393/148‑1 (MQ=255)
                                                                                                            tggATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCAGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTGAACGTTGCCACAGAATTAAAAATAGCCTTCGCTGGCGCGGTTAAAGCCTGGTTTGCGGaaaa                                         <  1:2810901/148‑1 (MQ=255)
                                                                                                             ggATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCCGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTGAACGTTGCCACAGAATTAAAAATAGCCTTCGCTGGCGCGGTTAAAGCCTGGTTTGCGGAAAAt                                        >  1:1936355/1‑148 (MQ=255)
                                                                                                                tGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCCGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTGAACGTTGCCACAGAATTAAAAATAGCCTTCGCTGGCGCGGTTAAAGCCTGGTTTGCGGAAAATCCg                                     >  1:454862/1‑148 (MQ=255)
                                                                                                                      tctGGTGCTGCATGGTGCCAGCGATGTTCAGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTGAACGTTGCCACAGAATTAAAAATAGCCTTCGCTGGCGCGGTTAAAGCCTGGTTTGCGGAAAATCCGCAGGGt                               >  1:3542870/1‑148 (MQ=255)
                                                                                                                        tGGTGCTGCATGGTGCCAGCGATGTTCCGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTGAACGTTGCCACAGAATTAAAAATAGCCTTCGCTGGCGCGGTTAAAGCCTGGTTTGCGGAAAATCCGCAGGGTaa                             <  1:1663025/148‑1 (MQ=255)
                                                                                                                           tgctgcATGGTGCCAGCGATGTTCCGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTGAACGTTGCCACAGAATTAAAAATAGCCTTCGCTGGCGCGGTTAAAGCCTGGTTTGCGGAAAATCCGCAGGGTAATGa                          >  1:1318187/1‑148 (MQ=255)
                                                                                                                               gcATGGTGCCAGCGATGTTCCGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTGAACGTTGCCACAGAATTAAAAATAGCCTTCGCTGGCGCGGTTAAAGCCTGGTTTGCGGAAAATCCGCAGGGTAATGATCCt                      <  1:665004/148‑1 (MQ=255)
                                                                                                                               gcATGGTGCCAGCGATGTTCCGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTGAACGTTGCCACAGAATTAAAAATAGCCTTCGCTGGCGCGGTTAAAGCCTGGTTTGCGGAAAATCCGCAGGGTAATGATCCt                      <  1:3434888/148‑1 (MQ=255)
                                                                                                                                  tGGTGCCAGCGATGTTCCGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTGAACGTTGCCACAGAATTAAAAATAGCCTTCGCTGGCGCGGTTAAAGCCTGGTTTGCGGAAAATCCGCAGGGTAATGATCCTCGt                   >  2:3093194/1‑148 (MQ=255)
                                                                                                                                  tGGTGCCAGCGATGTTCCGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTGAACGTTGCCACAGAATTAAAAATAGCCTTCGCTGGCGCGGTTAAAGCCTGGTTTGCGGAAAATCCGCAGGGTAATGATCCTCGt                   >  1:110191/1‑148 (MQ=255)
                                                                                                                                  tGGTGCCAGCGATGTTCAGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTGAACGTTGCCACAGAATTAAAAATAGCCTTCGCTGGCGCGGTTAAAGCCTGGTTTGCGGAAAATCCGCAGGGTAATGATCCTCGt                   >  2:2657576/1‑148 (MQ=255)
                                                                                                                                      gCCAGCGATGTTCAGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTGAACGTTGCCACAGAATTAAAAATAGCCTTCGCTGGCGCGGTTAAAGCCTGGTTTGCGGAAAATCCGCAGGGTAATGATCCTCGttatt               <  1:3336562/148‑1 (MQ=255)
                                                                                                                                        cAGCGATGTTCCGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTGAACGTTGCCACAGAATTAAAAATAGCCTTCGCTGGCGCGGTTAAAGCCTGGTTTGCGGAAAATCCGCAGGGTAATGATCCTCGTtattat             >  1:1694408/1‑148 (MQ=255)
                                                                                                                                          gcgcggTTCAGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACTACAGTGAACGTTGCCACAGAATTTTAAATAGACTTCGCTGGCGCGGTTAAAGCCTGGTTTGCGGAAAATCCGCAGGGTAATGATCCTCGTTATTatat           <  2:1897787/143‑1 (MQ=255)
                                                                                                                                          gCGATGTTCAGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACACAAGTGAACGGTGCCACAGAATTAAAAATAGCCTTCGCTGGCGCGGTTAAAGCCGGGTTTGCGGAAAATCCGCAGGGTAATGATCCTCGTTATTatat           <  2:2820451/148‑1 (MQ=255)
                                                                                                                                             aTGTTCCGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTGAACGTTGCCACAGAATTAAAAATAGCCTTCGCTGGCGCGGTTAAAGCCTGGTTTGCGGAAAATCCGCAGGGTAATGATCCTCGTTATTATATgcg        >  1:113462/1‑148 (MQ=255)
                                                                                                                                              tGTTCCGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTGAACGTTGCCACAGAATTAAAAATAGCCTTCGCTGGCGCGGTTAAAGCCTGGTTTGCGGAAAATCCGCAGGGTAATGATCCTCGTTATTATATgcgc       >  2:1235294/1‑148 (MQ=255)
                                                                                                                                                ttCAGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTGAACGTTGCCACAGAATTAAAAATAGCCTTCGCTGGCGCGGTTAAAGCCTGGTTTGCGGAAAATCCGCAGGGTAATGATCCTCGTTATTATATGCGCGt     >  2:2094060/1‑148 (MQ=255)
                                                                                                                                                   cGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTGAACGTTGCCACAGAATTAAAAATAGCCTTCGCTGGCGCGGTTAAAGCCTGGTTTGCGGAAAATCCGCAGGGTAATGATCCTCGTTATTATATGCGCGTCgg  <  1:10391/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
AACTGACTGGCGTCGACAGCCTGGCGGTAGCGATTGGTACGGCGCACGGCTTATACAGCAAAACGCCGAAGATTGATTTCCAGCGGCTGGCGGAAATTCGTGAAGTGGTGGATGTTCCTCTGGTGCTGCATGGTGCCAGCGATGTTCCGGATGAATTTGTCCGTCGCACTATTGAACTTGGCGTCACAAAAGTGAACGTTGCCACAGAATTAAAAATAGCCTTCGCTGGCGCGGTTAAAGCCTGGTTTGCGGAAAATCCGCAGGGTAATGATCCTCGTTATTATATGCGCGTCGG  >  CP009273/3276995‑3277289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A