Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,125,407 C→A 100% H549N (CAT→AAT)  katG → catalase‑peroxidase HPI, heme b‑containing

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,125,4070CA97.5% 642.7 / 6.1 203H549N (CAT→AAT) katGcatalase‑peroxidase HPI, heme b‑containing
Reads supporting (aligned to +/- strand):  ref base C (1/4);  new base A (95/103);  total (96/107)
Fisher's exact test for biased strand distribution p-value = 3.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 203 total aligned reads displayed.

GGATGTGAACGCCGCAGCCGTTCGTGCTCTGCCTGTTCTGGAGAAAATCCAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTCATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGTTTCCACCACCGAGTCACTGCTGATC  >  CP009273/4125262‑4125550
                                                                                                                                                 |                                                                                                                                               
   tgtgAACGCCGCAGCCGTTCGTGCTCTGCCTGTTCTGGAGAAAATCCAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTa                                                                                                                                            <  2:624012/148‑1 (MQ=255)
    gtgAACGCCGCAGCAGTTCGTGGTCTGCCTGTTCTGGAGAAAATCCAGTAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTAc                                                                                                                                           <  2:1070501/148‑1 (MQ=255)
      gAACGCCGCAGCCGTTCGTGCTCTGCCTGTTCTGGAGAAAATCCAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCg                                                                                                                                         <  1:2178641/148‑1 (MQ=255)
      gAACGCCGCAGCCGTTCGTGCTCTGCCTGTTCTGGAGAAAATCCAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCg                                                                                                                                         >  1:620072/1‑148 (MQ=255)
        aCGCCGCAGCCGTTCGTGCTCTGCCTGTTCTGGAGAAAATCCAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGtt                                                                                                                                       <  1:3096209/148‑1 (MQ=255)
              cAGCCGTTCGTGCTCTGCCTGTTCTGGAGAAAATCCAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGcc                                                                                                                                 >  1:3120467/1‑148 (MQ=255)
              cAGCCGTTCGTGCTCTGCCTGTTCTGGAGAAAATCCAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGcc                                                                                                                                 <  1:813471/148‑1 (MQ=255)
               aGCCGTTCGTGCTCTGCCTGTTCTGGAGAAAATCCAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCg                                                                                                                                <  2:218399/148‑1 (MQ=255)
                 ccGTTCGTGCTCTGCCTGTTCTGGAGAAAATCCAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCggg                                                                                                                              >  2:1132441/1‑148 (MQ=255)
                   gTTCGTGCTCTGCCTGTTCTGGAGAAAATCCAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTc                                                                                                                            >  2:1799061/1‑148 (MQ=255)
                     tCGTGCTCTGCCTGTTCTGGAGAAAATCCAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTcgc                                                                                                                          <  1:527195/148‑1 (MQ=255)
                         gCTCTGCCTGTTCTGGAGAAAATCCAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTCATGTACCGTTTGCGCCGGGTCGCGTTg                                                                                                                      <  2:3080271/148‑1 (MQ=255)
                            ctGCCTGTTCTGGAGAAAATCCAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATg                                                                                                                   >  2:434302/1‑148 (MQ=255)
                                cTGTTCTGGAGAAAATCCAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGcgcg                                                                                                               <  2:2830134/148‑1 (MQ=255)
                                cTGTTCTGGAGAAAATCCAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGcgcg                                                                                                               <  1:536639/148‑1 (MQ=255)
                                    tCTGGAGAAAATCCAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAg                                                                                                           >  2:2696626/1‑148 (MQ=255)
                                    tCTGGAGAAAATCCAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAg                                                                                                           <  1:1925986/148‑1 (MQ=255)
                                    tCTGGAGAAAATCCAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAg                                                                                                           >  2:2696708/1‑148 (MQ=255)
                                    tCTGGAGAAAATCCAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAg                                                                                                           >  2:444022/1‑148 (MQ=255)
                                    tCTGGAGAAAATCCAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAg                                                                                                           >  2:3084546/1‑148 (MQ=255)
                                     cTGGAGAAAATCCAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAgg                                                                                                          <  2:148955/148‑1 (MQ=255)
                                        gagaAAATCCAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATc                                                                                                       >  2:2426258/1‑148 (MQ=255)
                                        gagaAAATCCAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATc                                                                                                       >  2:2259241/1‑148 (MQ=255)
                                        gagaAAATCCAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATc                                                                                                       >  1:3230747/1‑148 (MQ=255)
                                           aaaaTCCAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGa                                                                                                    <  2:1301919/148‑1 (MQ=255)
                                                ccAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGAc                                                                                               <  2:2056295/148‑1 (MQ=255)
                                                 cAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACa                                                                                              <  1:3557899/148‑1 (MQ=255)
                                                 cAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACa                                                                                              <  1:327016/148‑1 (MQ=255)
                                                  aGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACAt                                                                                             >  1:3013119/1‑148 (MQ=255)
                                                  aGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACAt                                                                                             <  2:350883/148‑1 (MQ=255)
                                                  aGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACAt                                                                                             >  2:3345557/1‑148 (MQ=255)
                                                  aGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACAt                                                                                             <  2:514703/148‑1 (MQ=255)
                                                  aGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACAt                                                                                             >  1:1938243/1‑148 (MQ=255)
                                                    aaaGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTg                                                                                           <  1:370554/148‑1 (MQ=255)
                                                      agagTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTgag                                                                                         <  2:2405604/148‑1 (MQ=255)
                                                      agagTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTgag                                                                                         <  2:2405688/148‑1 (MQ=255)
                                                       gagTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTgaga                                                                                        >  2:586323/1‑148 (MQ=255)
                                                       gagTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTgaga                                                                                        >  2:408987/1‑148 (MQ=255)
                                                        agTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGAt                                                                                       >  2:2313237/1‑148 (MQ=255)
                                                         gTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATg                                                                                      <  2:1496926/148‑1 (MQ=255)
                                                          tCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGt                                                                                     <  2:3416835/148‑1 (MQ=255)
                                                           cTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGtt                                                                                    <  2:3013119/148‑1 (MQ=255)
                                                                  aGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAgctg                                                                             <  2:457028/148‑1 (MQ=255)
                                                                  aGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAgctg                                                                             >  1:969793/1‑148 (MQ=255)
                                                                     cTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGctgctg                                                                          >  2:2192081/1‑148 (MQ=255)
                                                                      tCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTgg                                                                         >  2:1363072/1‑148 (MQ=255)
                                                                      tCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTgg                                                                         >  2:1550168/1‑148 (MQ=255)
                                                                      tCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATNAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTgg                                                                         <  1:1801238/148‑1 (MQ=255)
                                                                       cGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGa                                                                        >  2:1371684/1‑148 (MQ=255)
                                                                            gCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCaa                                                                   <  2:488945/148‑1 (MQ=255)
                                                                             cGGATATCATTGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAAt                                                                  <  2:907762/148‑1 (MQ=255)
                                                                               gATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTg                                                                >  2:1721446/1‑148 (MQ=255)
                                                                               gATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTg                                                                <  2:2086440/148‑1 (MQ=255)
                                                                                atatCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGc                                                               <  2:2049769/148‑1 (MQ=255)
                                                                                atatCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGc                                                               >  2:3257389/1‑148 (MQ=255)
                                                                                    cATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGAc                                                           <  1:2882009/148‑1 (MQ=255)
                                                                                      tAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACgg                                                         >  1:1881060/1‑148 (MQ=255)
                                                                                      tAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACgg                                                         <  1:533550/148‑1 (MQ=255)
                                                                                       aGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGt                                                        <  1:1753352/148‑1 (MQ=255)
                                                                                        gTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGtt                                                       >  1:1710406/1‑148 (MQ=255)
                                                                                         tGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGttt                                                      <  1:3544459/148‑1 (MQ=255)
                                                                                               ctggTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTaa                                                >  1:3524970/1‑148 (MQ=255)
                                                                                                  gtgtGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTa                                             <  1:1721446/148‑1 (MQ=255)
                                                                                                   tgtgGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTAt                                            <  1:1967836/148‑1 (MQ=255)
                                                                                                    gtgGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATc                                           <  2:2608081/148‑1 (MQ=255)
                                                                                                      ggttggtGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATcgc                                         >  1:3552828/1‑148 (MQ=255)
                                                                                                        ttggtGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCgcgc                                       <  1:2780828/148‑1 (MQ=255)
                                                                                                            tgtTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGt                                   <  2:1581916/148‑1 (MQ=255)
                                                                                                             gtTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTc                                  >  1:1549719/1‑148 (MQ=255)
                                                                                                               tGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGGTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTg                                >  1:1273318/1‑148 (MQ=255)
                                                                                                                gagaAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTgg                               <  1:586323/148‑1 (MQ=255)
                                                                                                                 agaAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGa                              <  1:3345557/148‑1 (MQ=255)
                                                                                                                 agaAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGa                              >  1:2762991/1‑148 (MQ=255)
                                                                                                                 agaAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATGGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGa                              >  1:2763371/1‑148 (MQ=255)
                                                                                                                    aaGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGt                           <  1:2696659/148‑1 (MQ=255)
                                                                                                                     aGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGtt                          <  1:2426258/148‑1 (MQ=255)
                                                                                                                     aGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGtt                          >  2:1440216/1‑148 (MQ=255)
                                                                                                                     aGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGtt                          >  2:1653571/1‑148 (MQ=255)
                                                                                                                     aGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGtt                          >  2:1653514/1‑148 (MQ=255)
                                                                                                                      gCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGttt                         >  1:3002554/1‑148 (MQ=255)
                                                                                                                        cGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGTTTcc                       <  2:455785/148‑1 (MQ=255)
                                                                                                                         gCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGTTTcca                      <  1:2521630/148‑1 (MQ=255)
                                                                                                                         gCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGTTTcca                      <  1:1349886/148‑1 (MQ=255)
                                                                                                                          cAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGTTTccac                     <  2:951967/148‑1 (MQ=255)
                                                                                                                          cAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGTTTccac                     >  1:2025308/1‑148 (MQ=255)
                                                                                                                          cAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGTTTccac                     >  1:1134417/1‑148 (MQ=255)
                                                                                                                             gcgcCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGTTTCcaccac                  >  1:1178242/1‑148 (MQ=255)
                                                                                                                                ccgcAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGTTTCCACCACCGa               >  2:316640/1‑148 (MQ=255)
                                                                                                                                    aGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGTTTCCACCACCGAGTCa           <  2:3274257/148‑1 (MQ=255)
                                                                                                                                    aGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGTTTCCACCACCGAGTCa           <  1:777729/148‑1 (MQ=255)
                                                                                                                                    aGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGTTTCCACCACCGAGTCa           >  1:1118671/1‑148 (MQ=255)
                                                                                                                                     ggTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGTTTCCACCACCGAGTCAc          >  2:3375831/1‑148 (MQ=255)
                                                                                                                                      gTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGTTTCCACCACCGAGTCAct         >  2:2937025/1‑148 (MQ=255)
                                                                                                                                        ttGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGTTTCCACCACCGAGTCActgc       >  2:1958343/1‑148 (MQ=255)
                                                                                                                                          gAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGTTTCCACCACCGAGTCActgctg     <  1:449392/148‑1 (MQ=255)
                                                                                                                                          gAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGTTTCCACCACCGAGTCActgctg     <  1:2192081/148‑1 (MQ=255)
                                                                                                                                          gAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGTTTCCACCACCGAGTCActgctg     <  1:1334804/148‑1 (MQ=255)
                                                                                                                                           aGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGTTTCCACCACCGAGTCACTGCTGa    <  1:1453182/148‑1 (MQ=255)
                                                                                                                                             cATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGTTTCCACCACCGAGTCACTGCTGATc  >  2:1888112/1‑148 (MQ=255)
                                                                                                                                             cATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGTTTCCACCACCGAGTCACTGCTGATc  >  1:713237/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
GGATGTGAACGCCGCAGCCGTTCGTGCTCTGCCTGTTCTGGAGAAAATCCAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTCATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGTTTCCACCACCGAGTCACTGCTGATC  >  CP009273/4125262‑4125550

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TGGGATGTGAACGCCGCAGCCGTTCGTGCTCTGCCTGTTCTGGAGAAAATCCAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTCATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGTTTCCACCACCGAGTCACTGCTGATCGACA  >  CP009273/4125260‑4125554
                                                                                                                                                   |                                                                                                                                                   
TGGGATGTGAACGCCGCAGCCGTTCGTGCTCTGCCTGTTCTGGAGAAAATCCAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAAT                                                                                                                                                   >  SRR19973067.460191/1‑150 (MQ=60)
  GGATGTGAACGCCGCAGCCGTTCGTGCTCTGCCTGTTCTGGAGAAAATCCAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGT                                                                                                                                                 <  SRR19973067.1675430/150‑1 (MQ=60)
      GTGAACGCCGCAGCCGTTCGTGCTCTGCCTGTTCTGGAGAAAATCCAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGGGTGGGTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCG                                                                                                                                             >  SRR19973067.624151/1‑150 (MQ=60)
        GAACGCCGCAGCCGTTCGTGCTCTGCCTGTTCTGGAGAAAATCCAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTT                                                                                                                                           <  SRR19973067.2193213/150‑1 (MQ=60)
        GAACGCCGCAGCCGTTCGTGCTCTGCCTGTTCTGGAGAAAATCCAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTT                                                                                                                                           <  SRR19973067.2193815/150‑1 (MQ=60)
        GAACGCCGCAGCCGTTCGTGCTCTGCCTGTTCTGGAGAAAATCCAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTT                                                                                                                                           <  SRR19973067.2193209/150‑1 (MQ=60)
          CCGCCGCAGCCGTTCGTGCTCTGCCTGTTCTGGAGAAAATCCAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTG                                                                                                                                         <  SRR19973067.3121682/150‑1 (MQ=60)
          ACGCCGCAGCCGTTCGTGCTCTGCCTGTTCTGGAGAAAATCCAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTG                                                                                                                                         <  SRR19973067.3115901/150‑1 (MQ=60)
              CGCAGCCGTTCGTGCTCTGCCTGTTCTGGAGAAAATCCAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCC                                                                                                                                     >  SRR19973067.3140303/1‑150 (MQ=60)
              CGCAGCCGTTCGTGCTCTGCCTGTTCTGGAGAAAATCCAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCC                                                                                                                                     >  SRR19973067.1732005/1‑150 (MQ=60)
                CAGCCGTTCGTGCTCTGCCTGTTCTGGAGAAAATCCAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGG                                                                                                                                   <  SRR19973067.818978/150‑1 (MQ=60)
                CAGCCGTTCGTGCTCTGCCTGTTCTGGAGAAAATCCAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGG                                                                                                                                   <  SRR19973067.819050/150‑1 (MQ=60)
                       TCGTGCTCTGCCTGTTCTGGAGAAAATCCAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGT                                                                                                                            <  SRR19973067.530741/150‑1 (MQ=60)
                        CGTGCTCTGCCTGTTCTGGAGAAAATCCAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTT                                                                                                                           >  SRR19973067.883578/1‑150 (MQ=60)
                                  CTGTTCTGGAGAAAATCCAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTC                                                                                                                 <  SRR19973067.540224/150‑1 (MQ=60)
                                      TCTGGAGAAAATCCAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGA                                                                                                             <  SRR19973067.1939129/150‑1 (MQ=60)
                                      TCTGGAGAAAATCCAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGA                                                                                                             <  SRR19973067.3110788/150‑1 (MQ=60)
                                       tgctcttccgatctGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGAT                                                                                                            <  SRR19973067.1851737/136‑1 (MQ=60)
                                       CTGGAGAAAATCCAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGAT                                                                                                            <  SRR19973067.3399126/150‑1 (MQ=60)
                                        TGGAGAAAATCCAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATC                                                                                                           >  SRR19973067.3251024/1‑150 (MQ=60)
                                                  CCAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACAT                                                                                                 >  SRR19973067.3032281/1‑150 (MQ=60)
                                                  CCAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACAT                                                                                                 >  SRR19973067.3032290/1‑150 (MQ=60)
                                                  CCAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACAT                                                                                                 >  SRR19973067.1951460/1‑150 (MQ=60)
                                                   CAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCTTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATT                                                                                                >  SRR19973067.1213847/1‑150 (MQ=60)
                                                   CAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATT                                                                                                <  SRR19973067.3580312/150‑1 (MQ=60)
                                                   CAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATT                                                                                                <  SRR19973067.329248/150‑1 (MQ=60)
                                                   CAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATT                                                                                                <  SRR19973067.3579858/150‑1 (MQ=60)
                                                     GAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTCATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGA                                                                                              >  SRR19973067.753077/1‑150 (MQ=60)
                                                      AAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAG                                                                                             <  SRR19973067.373133/150‑1 (MQ=60)
                                                      AAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAG                                                                                             >  SRR19973067.1744460/1‑150 (MQ=60)
                                                      AAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATGGAG                                                                                             >  SRR19973067.2251380/1‑150 (MQ=60)
                                                       AAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGA                                                                                            >  SRR19973067.372774/1‑150 (MQ=60)
                                                                  AAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTG                                                                                 >  SRR19973067.976437/1‑150 (MQ=60)
                                                                  AAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTG                                                                                 >  SRR19973067.1164606/1‑150 (MQ=60)
                                                                        TCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAG                                                                           <  SRR19973067.572764/150‑1 (MQ=60)
                                                                        TCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATNAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAG                                                                           <  SRR19973067.1813345/150‑1 (MQ=60)
                                                                                  ATATCATAGTGGTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTG                                                                 <  SRR19973067.606073/150‑1 (MQ=60)
                                                                                    ATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGAC                                                               >  SRR19973067.1905123/1‑150 (MQ=60)
                                                                                      CATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGG                                                             <  SRR19973067.2900438/150‑1 (MQ=60)
                                                                                      CATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGG                                                             >  SRR19973067.1893896/1‑150 (MQ=60)
                                                                                        TAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTT                                                           <  SRR19973067.536999/150‑1 (MQ=60)
                                                                                        TAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTT                                                           <  SRR19973067.537124/150‑1 (MQ=60)
                                                                                        TAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTT                                                           >  SRR19973067.1722143/1‑150 (MQ=60)
                                                                                        TAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTT                                                           <  SRR19973067.537153/150‑1 (MQ=60)
                                                                                         AGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTT                                                          <  SRR19973067.1983625/150‑1 (MQ=60)
                                                                                         AGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTT                                                          <  SRR19973067.1765298/150‑1 (MQ=60)
                                                                                         AGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTT                                                          <  SRR19973067.1766245/150‑1 (MQ=60)
                                                                                           TGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCC                                                        <  SRR19973067.3566827/150‑1 (MQ=60)
                                                                                              TGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTA                                                     >  SRR19973067.1966185/1‑150 (MQ=60)
                                                                                               GGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAA                                                    >  SRR19973067.3547271/1‑150 (MQ=60)
                                                                                                    GTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATC                                               <  SRR19973067.2480151/150‑1 (MQ=60)
                                                                                                    GTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATC                                               <  SRR19973067.1733241/150‑1 (MQ=60)
                                                                                                     TGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCG                                              <  SRR19973067.1981191/150‑1 (MQ=60)
                                                                                                      GTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGC                                             >  SRR19973067.3575229/1‑150 (MQ=60)
                                                                                                      GTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGC                                             >  SRR19973067.3575188/1‑150 (MQ=60)
                                                                                                          TTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTC                                         <  SRR19973067.1130374/150‑1 (MQ=60)
                                                                                                          TTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTC                                         <  SRR19973067.2798779/150‑1 (MQ=60)
                                                                                                             GTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTC                                      >  SRR19973067.1560270/1‑150 (MQ=60)
                                                                                                              TGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCT                                     <  SRR19973067.68286/150‑1 (MQ=60)
                                                                                                               GTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTG                                    <  SRR19973067.3193614/150‑1 (MQ=60)
                                                                                                               GTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGGTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTG                                    >  SRR19973067.1281982/1‑150 (MQ=60)
                                                                                                                 TGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGA                                  >  SRR19973067.2781202/1‑150 (MQ=60)
                                                                                                                 TGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGA                                  >  SRR19973067.2780844/1‑150 (MQ=60)
                                                                                                                 TGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATGGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGA                                  >  SRR19973067.2781620/1‑150 (MQ=60)
                                                                                                                 TGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATGGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGA                                  >  SRR19973067.2781229/1‑150 (MQ=60)
                                                                                                                  GAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGAC                                 <  SRR19973067.590068/150‑1 (MQ=60)
                                                                                                                   AGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACG                                <  SRR19973067.3366754/150‑1 (MQ=60)
                                                                                                                     AAAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGTT                              >  SRR19973067.2959642/1‑150 (MQ=60)
                                                                                                                      AAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGTTT                             <  SRR19973067.2714005/150‑1 (MQ=60)
                                                                                                                      AAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGTTT                             >  SRR19973067.3021616/1‑150 (MQ=60)
                                                                                                                      AAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGTTT                             <  SRR19973067.2713972/150‑1 (MQ=60)
                                                                                                                      AAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGTTT                             <  SRR19973067.2714054/150‑1 (MQ=60)
                                                                                                                      AAGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGTTT                             >  SRR19973067.3338148/1‑150 (MQ=60)
                                                                                                                       AGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGTTTC                            <  SRR19973067.2442175/150‑1 (MQ=60)
                                                                                                                       AGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGTTTC                            <  SRR19973067.1551172/150‑1 (MQ=60)
                                                                                                                       AGCCGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGTTTC                            >  SRR19973067.1948275/1‑150 (MQ=60)
                                                                                                                          CGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGTTTCCAC                         >  SRR19973067.1142059/1‑150 (MQ=60)
                                                                                                                          CGCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGTTTCCAC                         >  SRR19973067.2038900/1‑150 (MQ=60)
                                                                                                                           GCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGTTTCCACC                        <  SRR19973067.1358074/150‑1 (MQ=60)
                                                                                                                           GCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGTTTCCACC                        <  SRR19973067.1359115/150‑1 (MQ=60)
                                                                                                                           GCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGTTTCCACC                        <  SRR19973067.1359121/150‑1 (MQ=60)
                                                                                                                           GCAAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGTTTCCACC                        <  SRR19973067.2538162/150‑1 (MQ=60)
                                                                                                                             AAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGTTTCCACCAC                      >  SRR19973067.1186028/1‑150 (MQ=60)
                                                                                                                             AAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGTTTCCACCAC                      >  SRR19973067.3563602/1‑150 (MQ=60)
                                                                                                                             AAGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGTTTCCACCAC                      >  SRR19973067.3563609/1‑150 (MQ=60)
                                                                                                                              AGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGTTTCCACCACC                     >  SRR19973067.130657/1‑150 (MQ=60)
                                                                                                                              AGCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGTTTCCACCACC                     >  SRR19973067.2877267/1‑150 (MQ=60)
                                                                                                                               GCGCCGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGTTTCCACCACCG                    <  SRR19973067.411838/150‑1 (MQ=60)
                                                                                                                                   CGCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATGGAGATGATTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGTTTCCACCACCGAGTC                <  SRR19973067.3281013/150‑1 (MQ=60)
                                                                                                                                    GCAGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGTTTCCACCACCGAGTCA               >  SRR19973067.1126244/1‑150 (MQ=60)
                                                                                                                                      AGGTTTGAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGTTTCCACCACCGAGTCACT             <  SRR19973067.783119/150‑1 (MQ=60)
                                                                                                                                            GAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGTTTCCACCACCGAGTCACTGCTGAT       <  SRR19973067.1343918/150‑1 (MQ=60)
                                                                                                                                            GAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGTTTCCACCACCGAGTCACTGCTGAT       <  SRR19973067.452512/150‑1 (MQ=60)
                                                                                                                                            GAGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGTTTCCACCACCGAGTCACTGCTGAT       <  SRR19973067.2206709/150‑1 (MQ=60)
                                                                                                                                             AGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGTTTCCACCACCGAGTCACTGCTGATC      <  SRR19973067.1462913/150‑1 (MQ=60)
                                                                                                                                             AGCATTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGTTTCCACCACCGAGTCACTGCTGATC      >  SRR19973067.718178/1‑150 (MQ=60)
                                                                                                                                                 TTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGTTTCCACCACCGAGTCACTGCTGATCGACA  <  SRR19973067.850364/150‑1 (MQ=60)
                                                                                                                                                 TTAATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGTTTCCACCACCGAGTCACTGCTGATCGACA  <  SRR19973067.3277748/150‑1 (MQ=60)
                                                                                                                                                   |                                                                                                                                                   
TGGGATGTGAACGCCGCAGCCGTTCGTGCTCTGCCTGTTCTGGAGAAAATCCAGAAAGAGTCTGGTAAAGCCTCGCTGGCGGATATCATAGTGCTGGCTGGTGTGGTTGGTGTTGAGAAAGCCGCAAGCGCCGCAGGTTTGAGCATTCATGTACCGTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGTTTCCACCACCGAGTCACTGCTGATCGACA  >  CP009273/4125260‑4125554

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑