Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 426,157 G→T 93.8% E33* (GAG→TAG)  yajD → HNH nuclease family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273426,1570GT93.8% 507.4 / 16.0 212E33* (GAG→TAG) yajDHNH nuclease family protein
Reads supporting (aligned to +/- strand):  ref base G (14/2);  new base T (97/98);  total (112/100)
Fisher's exact test for biased strand distribution p-value = 3.60e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 212 total aligned reads displayed.

ATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGCGGAT  >  CP009273/426013‑426300
                                                                                                                                                |                                                                                                                                               
aTCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAgt                                                                                                                                              <  1:2941039/148‑1 (MQ=255)
 tCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAggt                                                                                                                                             >  2:513876/1‑146 (MQ=255)
   ttGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAgtttg                                                                                                                                           <  1:1613437/148‑1 (MQ=255)
    tGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAgtttgt                                                                                                                                          <  2:3427317/148‑1 (MQ=255)
    tGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAgtttgt                                                                                                                                          >  2:1027093/1‑148 (MQ=255)
     gggAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAgtttgtt                                                                                                                                         <  1:3231003/148‑1 (MQ=255)
      ggAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAgtttgttt                                                                                                                                        >  1:1090627/1‑148 (MQ=255)
      ggAAACTGAGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAACCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAttttgttt                                                                                                                                        <  1:78190/148‑1 (MQ=255)
            tGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCa                                                                                                                                  >  2:3163434/1‑148 (MQ=255)
                cgTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCt                                                                                                                              <  2:3095522/148‑1 (MQ=255)
                     cccTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTg                                                                                                                         >  1:1133002/1‑148 (MQ=255)
                     cccTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTACAACCTGCGTg                                                                                                                         >  1:408026/1‑148 (MQ=255)
                       cTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGaa                                                                                                                       >  2:961327/1‑148 (MQ=255)
                       cTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGaa                                                                                                                       >  2:1353800/1‑148 (MQ=255)
                       cTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGaa                                                                                                                       >  2:1077579/1‑148 (MQ=255)
                          cATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACtt                                                                                                                    >  1:974934/1‑148 (MQ=255)
                          cATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAGTTTGTTTATTCCAACCTGCGTGAACtt                                                                                                                    >  1:2373535/1‑148 (MQ=255)
                           aTTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTa                                                                                                                   <  1:1642815/148‑1 (MQ=255)
                           aTTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTa                                                                                                                   <  1:812508/148‑1 (MQ=255)
                              tCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCg                                                                                                                <  1:3253654/148‑1 (MQ=255)
                                aTCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGtt                                                                                                              >  2:1038555/1‑148 (MQ=255)
                                 tCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGGTAGTTTGTTTTTTCCAACCTGCGTGAACTTACCGTTc                                                                                                             >  1:114878/1‑148 (MQ=255)
                                 tCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTc                                                                                                             <  2:2313490/148‑1 (MQ=255)
                                    aGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTcacc                                                                                                          >  2:2600126/1‑148 (MQ=255)
                                     ggTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTcacca                                                                                                         >  1:1631328/1‑148 (MQ=255)
                                       tAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACcaca                                                                                                       >  2:1335991/1‑148 (MQ=255)
                                        aaCTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACAt                                                                                                      <  1:434107/148‑1 (MQ=255)
                                            tttCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTACAACCTGCGTGAACTTACCGTTCACCACATTGAt                                                                                                  >  1:2430948/1‑148 (MQ=255)
                                             ttCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATc                                                                                                 <  2:134186/148‑1 (MQ=255)
                                                 tGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGa                                                                                             >  2:3202970/1‑148 (MQ=255)
                                                 tGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGa                                                                                             <  1:2904089/148‑1 (MQ=255)
                                                 tGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGa                                                                                             >  1:3216749/1‑148 (MQ=255)
                                                    cTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCa                                                                                          >  2:3136355/1‑148 (MQ=255)
                                                    cTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCa                                                                                          <  1:2659436/148‑1 (MQ=255)
                                                     tATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCAt                                                                                         >  2:1880501/1‑148 (MQ=255)
                                                      atcatcCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATa                                                                                        >  2:1759779/1‑148 (MQ=255)
                                                      atcatcCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATa                                                                                        <  1:2007287/148‑1 (MQ=255)
                                                      atcatcCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTACAACCTGCGTGAACTTACCGTTAACCACATTGATCACGACCATa                                                                                        >  1:1656618/1‑148 (MQ=255)
                                                       tcatcCCAAAAAACTATGCGCGGTTAGAAAGCGGCTCTCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATAc                                                                                       >  2:2153188/1‑148 (MQ=255)
                                                       tcatcCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATAc                                                                                       >  2:2804765/1‑148 (MQ=255)
                                                           cccAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTACAACCTGCGTGAACTTACCGTTAACCACAATGATCACGaacaaaacaaa                                                                                   >  1:970473/1‑147 (MQ=255)
                                                            ccAAAAAACTATGCGCGGTTATAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATa                                                                                  <  2:1374115/148‑1 (MQ=255)
                                                             cAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATaa                                                                                 <  1:2323407/148‑1 (MQ=255)
                                                                   aCTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGa                                                                           <  2:529416/148‑1 (MQ=255)
                                                                   aCTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGa                                                                           <  1:641450/148‑1 (MQ=255)
                                                                   aCTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACAATGATCACGACCATAACAATAAACCGGa                                                                           >  1:1029499/1‑148 (MQ=255)
                                                                     tATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAg                                                                         <  1:1198273/148‑1 (MQ=255)
                                                                       tGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTTAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTACAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGAt                                                                       <  2:3297850/148‑1 (MQ=255)
                                                                       tGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACGTACCGGTCACCACATTGATCACGACCATACCCATAAACCGGAACAt                                                                       >  1:2793275/1‑148 (MQ=255)
                                                                         cgcgGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATgg                                                                     <  1:1749767/148‑1 (MQ=255)
                                                                         cgcgGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATgg                                                                     <  2:1090627/148‑1 (MQ=255)
                                                                         cgcgGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGAACATACCAATAACCCGGAAGATgg                                                                     <  2:2654038/148‑1 (MQ=255)
                                                                          gcgGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGc                                                                    <  1:3027468/148‑1 (MQ=255)
                                                                           cgGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCa                                                                   >  2:924631/1‑148 (MQ=255)
                                                                           cgGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCa                                                                   >  2:2724037/1‑148 (MQ=255)
                                                                              ttAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTa                                                                >  2:2231818/1‑148 (MQ=255)
                                                                               tAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTaa                                                               <  1:1061865/148‑1 (MQ=255)
                                                                                aGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAAc                                                              <  1:3564917/148‑1 (MQ=255)
                                                                                 gAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACt                                                             >  2:588720/1‑148 (MQ=255)
                                                                                    aGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTggg                                                          >  2:3028338/1‑148 (MQ=255)
                                                                                    aGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACAATGATCACGACCATACCAATAACCCGGAAGATGGCAGTTACgggg                                                          >  1:3149977/1‑148 (MQ=255)
                                                                                    aGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCAGCGTGAACATACCGTTAACCACATTGATAAAGACCATACCAATAAACCCGAAGATGGCAGTAACTggg                                                          >  1:1368859/1‑148 (MQ=255)
                                                                                      cGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAGGTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTgggca                                                        >  1:2523677/1‑146 (MQ=255)
                                                                                       ggCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAAt                                                       <  2:2405184/148‑1 (MQ=255)
                                                                                         cTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTACCCCGAGTTTGTTTATTAAAAACTGCGTGAACTTACCGTTCAACAAATATATCACGACCAAACCAAAAACACAGAAGAAGGCAGGAACTGGGAAatg                                                     >  2:3304508/1‑148 (MQ=255)
                                                                                            tCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAAttgttg                                                  >  2:1546010/1‑148 (MQ=255)
                                                                                             cgcgAAAAAGCATTAAAACTCTATCCGTGGGTCTGCGGTCGCTGGTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATtgttgt                                                 <  1:546438/148‑1 (MQ=255)
                                                                                             cgcgAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATtgttgt                                                 >  2:675584/1‑148 (MQ=255)
                                                                                               cgAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTgtgt                                               >  2:1937227/1‑148 (MQ=255)
                                                                                                 aaaaaGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGtct                                             <  1:1027093/148‑1 (MQ=255)
                                                                                                 aaaaaGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGtct                                             <  1:1978523/148‑1 (MQ=255)
                                                                                                  aaaaGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGtctc                                            >  1:2572930/1‑148 (MQ=255)
                                                                                                  aaaaGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGtctc                                            <  1:1308432/148‑1 (MQ=255)
                                                                                                   aaaGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTctct                                           >  2:1150774/1‑148 (MQ=255)
                                                                                                   aaaGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGGTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTctct                                           <  2:1192828/148‑1 (MQ=255)
                                                                                                         ctAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGcc                                     >  1:579675/2‑148 (MQ=255)
                                                                                                              aaTCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTACAAACAGCGTGAACTTAACGTTCACCACATTGATAACGACCATACCAATAAAACGGAAGATTGCAGTAACTGGGAATTGTTGTGTGTCTATTTCCACGAt                                >  1:229983/1‑148 (MQ=255)
                                                                                                               aTCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATc                               >  2:948855/1‑148 (MQ=255)
                                                                                                               aTCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATc                               <  1:3439697/148‑1 (MQ=255)
                                                                                                               aTCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATc                               <  1:352362/148‑1 (MQ=255)
                                                                                                                tCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTAACCACATTTATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTTCCACGATCa                              >  2:2113820/1‑148 (MQ=255)
                                                                                                                   aTCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGa                           <  1:1353767/148‑1 (MQ=255)
                                                                                                                   aTCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGa                           <  2:452358/148‑1 (MQ=255)
                                                                                                                    tCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTACAACCAGCGTGAACATAACGTTCACCACAATGATAACGACCCAAACAAAAAACCGGAACATTGCAGTAACTGGGAATTGTTGTGTGTATATTTCCACCATCATGAg                          >  1:692792/1‑148 (MQ=255)
                                                                                                                         gggTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTc                     >  2:1378199/1‑148 (MQ=255)
                                                                                                                            tCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGaa                  <  1:3474116/148‑1 (MQ=255)
                                                                                                                                cGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAatat              <  1:2643305/148‑1 (MQ=255)
                                                                                                                                cGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAatat              <  1:2885957/148‑1 (MQ=255)
                                                                                                                                cGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAatat              <  2:1629855/148‑1 (MQ=255)
                                                                                                                                cGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAatat              <  2:1631443/148‑1 (MQ=255)
                                                                                                                                cGGTCGCTGTTCCCGCTAGTTTGTTGATTCCAACCGGCGTGAACTTACCGTTCACCACAGTGATCACGACCAGACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAatag              <  1:662359/148‑2 (MQ=255)
                                                                                                                                 ggTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAAtata             <  2:229983/148‑1 (MQ=255)
                                                                                                                                   tCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATAcc           >  2:2420629/1‑148 (MQ=255)
                                                                                                                                      cTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGaa        >  1:2495551/1‑148 (MQ=255)
                                                                                                                                        gTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGc      >  2:1526855/1‑148 (MQ=255)
                                                                                                                                         ttCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGCg     >  2:3400750/1‑148 (MQ=255)
                                                                                                                                          tCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGCgg    >  1:481221/1‑148 (MQ=255)
                                                                                                                                           cccGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGCGGa   <  2:2077708/148‑1 (MQ=255)
                                                                                                                                            ccGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGCGGAt  <  2:3216797/148‑1 (MQ=255)
                                                                                                                                            ccGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGCGGAt  <  2:3216749/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                               
ATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGCGGAT  >  CP009273/426013‑426300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGCGGATCAGT  >  CP009273/426013‑426304
                                                                                                                                                |                                                                                                                                                   
ATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTT                                                                                                                                                <  SRR19973066.2960586/150‑1 (MQ=60)
   TTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTT                                                                                                                                             <  SRR19973066.1625138/150‑1 (MQ=60)
   TTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTT                                                                                                                                             <  SRR19973066.1846673/150‑1 (MQ=60)
    TGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAACAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTT                                                                                                                                            <  SRR19973066.636023/150‑1 (MQ=60)
    TGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTT                                                                                                                                            >  SRR19973066.1098574/1‑150 (MQ=60)
    TGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTT                                                                                                                                            >  SRR19973066.2671843/1‑150 (MQ=60)
     GGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTA                                                                                                                                           <  SRR19973066.3252347/150‑1 (MQ=60)
     GGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTA                                                                                                                                           <  SRR19973066.787435/150‑1 (MQ=60)
     GGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAGTTTGTTTA                                                                                                                                           >  SRR19973066.977734/1‑150 (MQ=60)
      GGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGGTTGTTTTT                                                                                                                                          >  SRR19973066.2706014/1‑150 (MQ=60)
      GGAAACTGAGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAACCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTATTTTGTTTAT                                                                                                                                          <  SRR19973066.79100/150‑1 (MQ=60)
            TGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAAC                                                                                                                                    <  SRR19973066.2932215/150‑1 (MQ=60)
                   AACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTG                                                                                                                             >  SRR19973066.1141325/1‑150 (MQ=60)
                   AACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTG                                                                                                                             >  SRR19973066.1141124/1‑150 (MQ=60)
                   AACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTACAACCTGCGTG                                                                                                                             >  SRR19973066.411233/1‑150 (MQ=60)
                      CCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAAC                                                                                                                          <  SRR19973066.2020787/150‑1 (MQ=60)
                        TACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCAGCGTGAACTT                                                                                                                        >  SRR19973066.982107/1‑150 (MQ=60)
                        TACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAGTTTGTTTATTCCAACCTGCGTGAACTT                                                                                                                        >  SRR19973066.2389571/1‑150 (MQ=60)
                           ATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACC                                                                                                                     <  SRR19973066.818465/150‑1 (MQ=60)
                           ATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACC                                                                                                                     <  SRR19973066.1654769/150‑1 (MQ=60)
                            TTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCG                                                                                                                    <  SRR19973066.3486311/150‑1 (MQ=60)
                              TCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTT                                                                                                                  <  SRR19973066.3275077/150‑1 (MQ=60)
                               CATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGGTAGTTTGTTTTTTCCAACCTGCGTGAACTTACCGTTC                                                                                                                 >  SRR19973066.116048/1‑150 (MQ=60)
                               CATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTC                                                                                                                 <  SRR19973066.833274/150‑1 (MQ=60)
                               CATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTC                                                                                                                 >  SRR19973066.533281/1‑150 (MQ=60)
                               CATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCAGCGTGAACTTACCGGTC                                                                                                                 >  SRR19973066.533285/1‑150 (MQ=60)
                                   CAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTACAACCTGCGTGAACTTACCGTTCACCA                                                                                                             >  SRR19973066.1643181/1‑150 (MQ=60)
                                   CAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTACAACCAGCGTGAACTTACCGTTCACCA                                                                                                             >  SRR19973066.1641683/1‑150 (MQ=60)
                                   CAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCAAGTTTGTTTATTACAACCGGCGTGAACATAACGTTCACCA                                                                                                             >  SRR19973066.1643298/1‑150 (MQ=60)
                                     GGTAACTATTCATGGCTATCATCCCAAAAAACTATGCGCGGTTATAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGACTTTGTTTATTCCAACCTGCGTGACCTTACAGTTCACCACA                                                                                                           >  SRR19973066.1718125/1‑150 (MQ=60)
                                      GTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTACAACCTGCGTGAACTTACCGTTCACCACAT                                                                                                          >  SRR19973066.3404861/1‑150 (MQ=60)
                                        AACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTG                                                                                                        <  SRR19973066.437552/150‑1 (MQ=60)
                                        AACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTG                                                                                                        <  SRR19973066.437470/150‑1 (MQ=60)
                                        AACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTG                                                                                                        <  SRR19973066.437135/150‑1 (MQ=60)
                                         ACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGA                                                                                                       >  SRR19973066.3568939/1‑150 (MQ=60)
                                         ACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAGTTTGTTTATTCCAACCAGCGTGAACTTACCGTTCACCACATTGA                                                                                                       >  SRR19973066.1056030/1‑150 (MQ=60)
                                          CTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTACAACCTGCGTGAACTTACCGTTCACCACATTGAT                                                                                                      >  SRR19973066.2447492/1‑150 (MQ=60)
                                           TTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATC                                                                                                     <  SRR19973066.1300736/150‑1 (MQ=60)
                                              TCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACG                                                                                                  >  SRR19973066.3258449/1‑150 (MQ=60)
                                               CATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGA                                                                                                 >  SRR19973066.3238044/1‑150 (MQ=60)
                                               CATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACAATGATCACGA                                                                                                 >  SRR19973066.3238086/1‑150 (MQ=60)
                                               CATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGATGTTACACCTAGTTTTTTTATTCAAACCAGCGTGAACTTACacaacaccaaattgtaaacga                                                                                                 >  SRR19973066.3238094/1‑129 (MQ=60)
                                                 TGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACC                                                                                               <  SRR19973066.2204114/150‑1 (MQ=60)
                                                 TGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACC                                                                                               <  SRR19973066.2923488/150‑1 (MQ=60)
                                                    CTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATA                                                                                            <  SRR19973066.2677262/150‑1 (MQ=60)
                                                    CTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTAACGTTCACCACAATGATAACGACCATA                                                                                            >  SRR19973066.1668289/1‑150 (MQ=60)
                                                    CTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTACAACCTGCGTGAACTTACCGTTAACCACATTGATCACGACCATA                                                                                            >  SRR19973066.1668686/1‑150 (MQ=60)
                                                      ATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACC                                                                                          <  SRR19973066.2021369/150‑1 (MQ=60)
                                                         ATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTACAACCTGCGTGAACTTACCGTTAACCACAATGATCACGAacaaaacaaa                                                                                       >  SRR19973066.977614/1‑140 (MQ=60)
                                                         ATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTACAACCTGCGTGAAATTAACGTTCACCACATTGATAACGACCATACCAAA                                                                                       >  SRR19973066.977756/1‑150 (MQ=60)
                                                             CAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACC                                                                                   <  SRR19973066.2339275/150‑1 (MQ=60)
                                                               AAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACGTACCGGTCACCACATTGATCACGACCATACCAATAACCCG                                                                                 >  SRR19973066.252347/1‑150 (MQ=60)
                                                                 AAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGA                                                                               >  SRR19973066.3253168/1‑150 (MQ=60)
                                                                 AAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACAATGATCACGACCATAACAATAAACCGGA                                                                               >  SRR19973066.1037083/1‑150 (MQ=60)
                                                                   ACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAG                                                                             <  SRR19973066.646089/150‑1 (MQ=60)
                                                                     TATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGAT                                                                           <  SRR19973066.1206451/150‑1 (MQ=60)
                                                                     TATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGAT                                                                           <  SRR19973066.1206635/150‑1 (MQ=60)
                                                                     TATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACGTACCGGTCACCACATTGATCACGACCATACCCATAAACCGGAACAT                                                                           >  SRR19973066.2812072/1‑150 (MQ=60)
                                                                      ATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATG                                                                          <  SRR19973066.651277/150‑1 (MQ=60)
                                                                         CGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCA                                                                       <  SRR19973066.1762356/150‑1 (MQ=60)
                                                                          GCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAG                                                                      <  SRR19973066.3047659/150‑1 (MQ=60)
                                                                          GCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAG                                                                      <  SRR19973066.3047686/150‑1 (MQ=60)
                                                                           CGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGT                                                                     <  SRR19973066.2828487/150‑1 (MQ=60)
                                                                               TAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACT                                                                 <  SRR19973066.1069652/150‑1 (MQ=60)
                                                                                AGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTG                                                                <  SRR19973066.3588553/150‑1 (MQ=60)
                                                                                  AAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACAATGATCACGACCATACCAATAACCCGGAAGATGGCAGTTACGGGG                                                              >  SRR19973066.3170980/1‑150 (MQ=60)
                                                                                  AAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCAGCGTGAACATACCGTTAACCACATTGATAAAGACCATACCAATAAACCCGAAGATGGCAGTAACTGGG                                                              >  SRR19973066.1378762/1‑150 (MQ=60)
                                                                                   AAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGA                                                             <  SRR19973066.671245/150‑1 (MQ=60)
                                                                                    AGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAGGTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGCA                                                            >  SRR19973066.2540842/1‑150 (MQ=60)
                                                                                         CTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTT                                                       <  SRR19973066.35511/150‑1 (MQ=60)
                                                                                         CTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTT                                                       <  SRR19973066.2320780/150‑1 (MQ=60)
                                                                                           ATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGT                                                     <  SRR19973066.3204005/150‑1 (MQ=60)
                                                                                           ATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAAAAAACCGGAAGATGGCAGTAACTGGGAATTGTTTT                                                     >  SRR19973066.2563953/1‑150 (MQ=60)
                                                                                           ATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTAACGTTCACCACATTTATCACGACCATAACAAAAACCCGGAAGATGGCAGTAACTGGGGATTGTTTT                                                     >  SRR19973066.1343730/1‑150 (MQ=60)
                                                                                           ATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTACAACCAGCGTGAACTTAACGTTAACCACATTTATCACGACCATAACAAAAACCCCGAACATGGCAGTAAATTGGAATTGTTTT                                                     >  SRR19973066.82042/1‑150 (MQ=60)
                                                                                             CGCGAAAAAGCATTAAAACTCTATCCGTGGGTCTGCGGTCGCTGGTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGT                                                   <  SRR19973066.550377/150‑1 (MQ=60)
                                                                                             CGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGT                                                   <  SRR19973066.550391/150‑1 (MQ=60)
                                                                                              GCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAGTTTGTTTATTCCAACCAGCGTGAACTTACCGTTAACCACATTGATAACGACCATACCAATAAACCGGAAGATGGCCGTAACTGGGAATTGTTGTGTC                                                  >  SRR19973066.1100701/1‑150 (MQ=60)
                                                                                                GAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTC                                                >  SRR19973066.2590385/1‑150 (MQ=60)
                                                                                                 AAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCT                                               <  SRR19973066.1035234/150‑1 (MQ=60)
                                                                                                 AAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCT                                               <  SRR19973066.1034652/150‑1 (MQ=60)
                                                                                                 AAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCT                                               <  SRR19973066.1992497/150‑1 (MQ=60)
                                                                                                  AAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTA                                              <  SRR19973066.2288702/150‑1 (MQ=60)
                                                                                                  AAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTA                                              <  SRR19973066.1317924/150‑1 (MQ=60)
                                                                                                       CACTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCC                                         >  SRR19973066.583743/1‑150 (MQ=60)
                                                                                                        ATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTGCCGCGAGTTTGTTTTTTTCAACCTGCGTGTACTTACCGTTCACCCCAATGATAACGACCAAACCAAAAACCCGGAAGATGGGAGTAACTGGGCATTGTTttgtgtgtatttcca                                        >  SRR19973066.2711576/1‑135 (MQ=60)
                                                                                                            AAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTACAAACAGCGTGAACTTAACGTTCACCACATTGATAACGACCATACCAATAAAACGGAAGATTGCAGTAACTGGGAATTGTTGTGTGTCTATTTCCACGAT                                    >  SRR19973066.231815/1‑150 (MQ=60)
                                                                                                             AAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATC                                   <  SRR19973066.3168860/150‑1 (MQ=60)
                                                                                                             AAATCTATCCGTGGGTCTGCGGTCGCTGGTGCCGCTAGGTTGTTTATTGCCAACTGCCTGTACTTACCGTTGTCCACATTTATCACGACCATACCCATAACCCGGAAGATGGCCGTAACTGGGACTTGGTGTGTGTGGATTGGCACGATC                                   >  SRR19973066.2425339/1‑150 (MQ=44)
                                                                                                               ATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCAT                                 >  SRR19973066.2670564/1‑150 (MQ=60)
                                                                                                               ATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCAT                                 <  SRR19973066.409134/150‑1 (MQ=60)
                                                                                                               ATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCAT                                 <  SRR19973066.355108/150‑1 (MQ=60)
                                                                                                               ATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCAT                                 <  SRR19973066.3462708/150‑1 (MQ=60)
                                                                                                                TCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATG                                <  SRR19973066.2616930/150‑1 (MQ=60)
                                                                                                                  TATCCGTGGGTCTGCGGTCGCTGTTGCCGCTAGTTTGTTTtatgaaaccatccttaaaaatcacttcacaccaattataacgacccaaccaaaaaaccggaaaatggcagtaaatgggaattgttttttatctattgacacgataaagat                              >  SRR19973066.3104386/1‑40 (MQ=54)
                                                                                                                  TATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTACAACCAGCGTGAACATAACGTTCACCACAATGATAACGACCCAAACAAAAAACCGGAACATTGCAGTAACTGGGAATTGTTGTGTGTATATTTCCACCATCATGAG                              >  SRR19973066.697898/1‑150 (MQ=53)
                                                                                                                   ATCCGTGGGTCTGCGGTCGCTGTTGCCGCGAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACCTTGATCACGACCCAACCAATAACCCGGAAGATGGCCGTAACTGGGACTTGTTGTGTGTCTATTTCCACGATCATGAGC                             >  SRR19973066.1371314/1‑150 (MQ=60)
                                                                                                                   ATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTGCAACCAGCGTGAACATACCGTTCACCACATTTATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGTATTGTTGTGTGTCTATTTCCACGATCATGAGC                             >  SRR19973066.1093160/1‑150 (MQ=60)
                                                                                                                   ATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGC                             <  SRR19973066.1363573/150‑1 (MQ=60)
                                                                                                                   ATCCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGC                             <  SRR19973066.1363606/150‑1 (MQ=60)
                                                                                                                     CCGTGGGTCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCAT                           <  SRR19973066.2908611/150‑1 (MQ=60)
                                                                                                                            TCTGCGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAAT                    <  SRR19973066.3497314/150‑1 (MQ=60)
                                                                                                                                CGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATAC                <  SRR19973066.2661076/150‑1 (MQ=60)
                                                                                                                                CGGTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATAC                <  SRR19973066.2905272/150‑1 (MQ=60)
                                                                                                                                CGGTCGCTGTTCCCGCTAGTTTGTTGATTCCAACCGGCGTGAACTTACCGTTCACCACAGTGATCACGACCAGACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATAGAC                <  SRR19973066.667197/150‑1 (MQ=60)
                                                                                                                                  GTCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCG              >  SRR19973066.2484887/1‑150 (MQ=60)
                                                                                                                                   TCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGA             <  SRR19973066.2430132/150‑1 (MQ=60)
                                                                                                                                   TCGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGA             >  SRR19973066.1474911/1‑150 (MQ=60)
                                                                                                                                    CGCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAA            >  SRR19973066.2512563/1‑150 (MQ=60)
                                                                                                                                     GCTGTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAG           >  SRR19973066.2380441/1‑150 (MQ=60)
                                                                                                                                        GTTCCCGCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGCGG        >  SRR19973066.484842/1‑150 (MQ=60)
                                                                                                                                              GCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGCGGATCAGT  >  SRR19973066.2838243/1‑150 (MQ=60)
                                                                                                                                              GCTAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGCGGATCAGT  >  SRR19973066.364033/1‑150 (MQ=60)
                                                                                                                                                |                                                                                                                                                   
ATCTTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCGCTGTTCCCGCGAGTTTGTTTATTCCAACCTGCGTGAACTTACCGTTCACCACATTGATCACGACCATACCAATAACCCGGAAGATGGCAGTAACTGGGAATTGTTGTGTCTCTATTGCCACGATCATGAGCATTCGAAATATACCGAAGCGGATCAGT  >  CP009273/426013‑426304

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑